/***************************************************************************** annotateMain.cpp (c) 2009 - Aaron Quinlan Hall Laboratory Department of Biochemistry and Molecular Genetics University of Virginia aaronquinlan@gmail.com Licenced under the GNU General Public License 2.0 license. ******************************************************************************/ #include "annotateBed.h" #include "version.h" using namespace std; // define the version #define PROGRAM_NAME "bedtools annotate" // define our parameter checking macro #define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) // function declarations void annotate_help(void); int annotate_main(int argc, char* argv[]) { // our configuration variables bool showHelp = false; // input file string mainFile; // parm flags bool sameStrand = false; bool diffStrand = false; bool haveBed = false; bool haveFiles = false; bool haveTitles = false; bool reportCounts = false; bool reportBoth = false; // list of annotation files / names vector inputFiles; vector inputTitles; // check to see if we should print out some help if(argc <= 1) showHelp = true; for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if((PARAMETER_CHECK("-h", 2, parameterLength)) || (PARAMETER_CHECK("--help", 5, parameterLength))) { showHelp = true; } } if(showHelp) annotate_help(); // do some parsing (all of these parameters require 2 strings) for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if(PARAMETER_CHECK("-i", 2, parameterLength)) { if ((i+1) < argc) { haveBed = true; mainFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-files", 6, parameterLength)) { if ((i+1) < argc) { haveFiles = true; i = i+1; string file = argv[i]; while (file[0] != '-' && i < argc) { inputFiles.push_back(file); i++; if (i < argc) file = argv[i]; } i--; } } else if(PARAMETER_CHECK("-names", 6, parameterLength)) { if ((i+1) < argc) { haveTitles = true; i = i+1; string title = argv[i]; while (title[0] != '-' && i < argc) { inputTitles.push_back(title); i++; if (i < argc) title = argv[i]; } i--; } } else if(PARAMETER_CHECK("-counts", 7, parameterLength)) { reportCounts = true; } else if(PARAMETER_CHECK("-both", 5, parameterLength)) { reportBoth = true; } else if (PARAMETER_CHECK("-s", 2, parameterLength)) { sameStrand = true; } else if (PARAMETER_CHECK("-S", 2, parameterLength)) { diffStrand = true; } else { cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; showHelp = true; } } // make sure we have both input files if (!haveBed || !haveFiles) { cerr << endl << "*****" << endl << "*****ERROR: Need -i and -files files. " << endl << "*****" << endl; showHelp = true; } if (sameStrand && diffStrand) { cerr << endl << "*****" << endl << "*****ERROR: Request either -s OR -S, not both." << endl << "*****" << endl; showHelp = true; } if (!showHelp) { BedAnnotate *ba = new BedAnnotate(mainFile, inputFiles, inputTitles, sameStrand, diffStrand, reportCounts, reportBoth); ba->AnnotateBed(); delete ba; return 0; } else { annotate_help(); return 0; } } void annotate_help(void) { cerr << "\nTool: bedtools annotate (aka annotateBed)" << endl; cerr << "Version: " << VERSION << "\n"; cerr << "Summary: Annotates the depth & breadth of coverage of features from mult. files" << endl; cerr << "\t on the intervals in -i." << endl << endl; cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -i -files FILE1 FILE2..FILEn" << endl << endl; cerr << "Options: " << endl; cerr << "\t-names\t" << "A list of names (one / file) to describe each file in -i." << endl; cerr << "\t\tThese names will be printed as a header line." << endl << endl; cerr << "\t-counts\t" << "Report the count of features in each file that overlap -i." << endl; cerr << "\t\t- Default is to report the fraction of -i covered by each file." << endl << endl; cerr << "\t-both\t" << "Report the counts followed by the % coverage." << endl; cerr << "\t\t- Default is to report the fraction of -i covered by each file." << endl << endl; cerr << "\t-s\t" << "Require same strandedness. That is, only counts overlaps" << endl; cerr << "\t\ton the _same_ strand." << endl; cerr << "\t\t- By default, overlaps are counted without respect to strand." << endl << endl; cerr << "\t-S\t" << "Require different strandedness. That is, only count overlaps" << endl; cerr << "\t\ton the _opposite_ strand." << endl; cerr << "\t\t- By default, overlaps are counted without respect to strand." << endl << endl; exit(1); }