/***************************************************************************** shiftBedMain.cpp (c) 2016 - David Richardson European Molecular Biology Laboratory, European Bioinformatics Institute davidr@ebi.ac.uk Licenced under the GNU General Public License 2.0 license. ******************************************************************************/ #include "shiftBed.h" #include "version.h" using namespace std; // define our program name #define PROGRAM_NAME "bedtools shift" // define our parameter checking macro #define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) // function declarations void shift_help(void); int shift_main(int argc, char* argv[]) { // our configuration variables bool showHelp = false; // input files string bedFile = "stdin"; string genomeFile; bool haveBed = true; bool haveGenome = false; bool havePlus = false; bool haveMinus = false; bool haveAll = false; float shiftPlus = 0.0; float shiftMinus = 0.0; bool fractional = false; bool printHeader = false; for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if((PARAMETER_CHECK("-h", 2, parameterLength)) || (PARAMETER_CHECK("--help", 5, parameterLength))) { showHelp = true; } } if(showHelp) shift_help(); // do some parsing (all of these parameters require 2 strings) for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if(PARAMETER_CHECK("-i", 2, parameterLength)) { if ((i+1) < argc) { bedFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-g", 2, parameterLength)) { if ((i+1) < argc) { haveGenome = true; genomeFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-p", 2, parameterLength)) { if ((i+1) < argc) { havePlus = true; shiftPlus = atof(argv[i + 1]); i++; } } else if(PARAMETER_CHECK("-m", 2, parameterLength)) { if ((i+1) < argc) { haveMinus = true; shiftMinus = atof(argv[i + 1]); i++; } } else if(PARAMETER_CHECK("-s", 2, parameterLength)) { if ((i+1) < argc) { haveAll = true; shiftPlus = atof(argv[i + 1]); shiftMinus = atof(argv[i + 1]); i++; } } else if(PARAMETER_CHECK("-pct", 4, parameterLength)) { fractional = true; } else if(PARAMETER_CHECK("-header", 7, parameterLength)) { printHeader = true; } else { cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; showHelp = true; } } // make sure we have both input files if (!haveBed || !haveGenome) { cerr << endl << "*****" << endl << "*****ERROR: Need both a BED (-i) and a genome (-g) file. " << endl << "*****" << endl; showHelp = true; } if ((haveMinus && havePlus) == haveAll) { cerr << endl << "*****" << endl << "*****ERROR: Need -m and -p together or -s alone. " << endl << "*****" << endl; showHelp = true; } if ((!havePlus && haveMinus) || (havePlus && !haveMinus)) { cerr << endl << "*****" << endl << "*****ERROR: Need both -m and -p. " << endl << "*****" << endl; showHelp = true; } if (!showHelp) { BedShift *bc = new BedShift(bedFile, genomeFile, shiftMinus, shiftPlus, fractional, printHeader); delete bc; return 0; } else { shift_help(); } return 0; } void shift_help(void) { cerr << "\nTool: bedtools shift (aka shiftBed)" << endl; cerr << "Version: " << VERSION << "\n"; cerr << "Summary: Shift each feature by requested number of base pairs." << endl << endl; cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -i -g [-s or (-p and -m)]" << endl << endl; cerr << "Options: " << endl; cerr << "\t-s\t" << "Shift the BED/GFF/VCF entry -s base pairs." << endl; cerr << "\t\t- (Integer) or (Float, e.g. 0.1) if used with -pct." << endl << endl; cerr << "\t-p\t" << "Shift features on the + strand by -p base pairs." << endl; cerr << "\t\t- (Integer) or (Float, e.g. 0.1) if used with -pct." << endl << endl; cerr << "\t-m\t" << "Shift features on the - strand by -m base pairs." << endl; cerr << "\t\t- (Integer) or (Float, e.g. 0.1) if used with -pct." << endl << endl; cerr << "\t-pct\t" << "Define -s, -m and -p as a fraction of the feature's length." << endl; cerr << "\t\tE.g. if used on a 1000bp feature, -s 0.50, " << endl; cerr << "\t\twill shift the feature 500 bp \"upstream\". Default = false." << endl << endl; cerr << "\t-header\t" << "Print the header from the input file prior to results." << endl << endl; cerr << "Notes: " << endl; cerr << "\t(1) Starts will be set to 0 if options would force it below 0." << endl; cerr << "\t(2) Ends will be set to the chromosome length if requested slop would" << endl; cerr << "\tforce it above the max chrom length." << endl; cerr << "\t(3) The genome file should tab delimited and structured as follows:" << endl; cerr << "\n\t" << endl << endl; cerr << "\tFor example, Human (hg19):" << endl; cerr << "\tchr1\t249250621" << endl; cerr << "\tchr2\t243199373" << endl; cerr << "\t..." << endl; cerr << "\tchr18_gl000207_random\t4262" << endl << endl; cerr << "Tips: " << endl; cerr << "\tOne can use the UCSC Genome Browser's MySQL database to extract" << endl; cerr << "\tchromosome sizes. For example, H. sapiens:" << endl << endl; cerr << "\tmysql --user=genome --host=genome-mysql.cse.ucsc.edu -A -e \\" << endl; cerr << "\t\"select chrom, size from hg19.chromInfo\" > hg19.genome" << endl << endl; // end the program here exit(1); }