/***************************************************************************** slopBedMain.cpp (c) 2009 - Aaron Quinlan Hall Laboratory Department of Biochemistry and Molecular Genetics University of Virginia aaronquinlan@gmail.com Licenced under the GNU General Public License 2.0 license. ******************************************************************************/ #include "slopBed.h" #include "version.h" using namespace std; // define our program name #define PROGRAM_NAME "bedtools slop" // define our parameter checking macro #define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) // function declarations void slop_help(void); int slop_main(int argc, char* argv[]) { // our configuration variables bool showHelp = false; // input files string bedFile = "stdin"; string genomeFile; bool haveBed = true; bool haveGenome = false; bool haveLeft = false; bool haveRight = false; bool haveBoth = false; bool forceStrand = false; float leftSlop = 0.0; float rightSlop = 0.0; bool fractional = false; bool printHeader = false; for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if((PARAMETER_CHECK("-h", 2, parameterLength)) || (PARAMETER_CHECK("--help", 5, parameterLength))) { showHelp = true; } } if(showHelp) slop_help(); // do some parsing (all of these parameters require 2 strings) for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if(PARAMETER_CHECK("-i", 2, parameterLength)) { if ((i+1) < argc) { bedFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-g", 2, parameterLength)) { if ((i+1) < argc) { haveGenome = true; genomeFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-l", 2, parameterLength)) { if ((i+1) < argc) { haveLeft = true; leftSlop = atof(argv[i + 1]); i++; } } else if(PARAMETER_CHECK("-r", 2, parameterLength)) { if ((i+1) < argc) { haveRight = true; rightSlop = atof(argv[i + 1]); i++; } } else if(PARAMETER_CHECK("-b", 2, parameterLength)) { if ((i+1) < argc) { haveBoth = true; leftSlop = atof(argv[i + 1]); rightSlop = atof(argv[i + 1]); i++; } } else if(PARAMETER_CHECK("-s", 2, parameterLength)) { forceStrand = true; } else if(PARAMETER_CHECK("-pct", 4, parameterLength)) { fractional = true; } else if(PARAMETER_CHECK("-header", 7, parameterLength)) { printHeader = true; } else { cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; showHelp = true; } } // make sure we have both input files if (!haveBed || !haveGenome) { cerr << endl << "*****" << endl << "*****ERROR: Need both a BED (-i) and a genome (-g) file. " << endl << "*****" << endl; showHelp = true; } if (!haveLeft && !haveRight && !haveBoth) { cerr << endl << "*****" << endl << "*****ERROR: Need -l and -r together or -b alone. " << endl << "*****" << endl; showHelp = true; } if ((!haveLeft && haveRight) || (haveLeft && !haveRight)) { cerr << endl << "*****" << endl << "*****ERROR: Need both -l and -r. " << endl << "*****" << endl; showHelp = true; } if (forceStrand && ((!(haveLeft) || !(haveRight)) && (!haveBoth))) { cerr << endl << "*****" << endl << "*****ERROR: Must supply -l and -r or just -b with -s. " << endl << "*****" << endl; showHelp = true; } if (!showHelp) { BedSlop *bc = new BedSlop(bedFile, genomeFile, forceStrand, leftSlop, rightSlop, fractional, printHeader); delete bc; return 0; } else { slop_help(); } return 0; } void slop_help(void) { cerr << "\nTool: bedtools slop (aka slopBed)" << endl; cerr << "Version: " << VERSION << "\n"; cerr << "Summary: Add requested base pairs of \"slop\" to each feature." << endl << endl; cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -i -g [-b or (-l and -r)]" << endl << endl; cerr << "Options: " << endl; cerr << "\t-b\t" << "Increase the BED/GFF/VCF entry -b base pairs in each direction." << endl; cerr << "\t\t- (Integer) or (Float, e.g. 0.1) if used with -pct." << endl << endl; cerr << "\t-l\t" << "The number of base pairs to subtract from the start coordinate." << endl; cerr << "\t\t- (Integer) or (Float, e.g. 0.1) if used with -pct." << endl << endl; cerr << "\t-r\t" << "The number of base pairs to add to the end coordinate." << endl; cerr << "\t\t- (Integer) or (Float, e.g. 0.1) if used with -pct." << endl << endl; cerr << "\t-s\t" << "Define -l and -r based on strand." << endl; cerr << "\t\tE.g. if used, -l 500 for a negative-stranded feature, " << endl; cerr << "\t\tit will add 500 bp downstream. Default = false." << endl << endl; cerr << "\t-pct\t" << "Define -l and -r as a fraction of the feature's length." << endl; cerr << "\t\tE.g. if used on a 1000bp feature, -l 0.50, " << endl; cerr << "\t\twill add 500 bp \"upstream\". Default = false." << endl << endl; cerr << "\t-header\t" << "Print the header from the input file prior to results." << endl << endl; cerr << "Notes: " << endl; cerr << "\t(1) Starts will be set to 0 if options would force it below 0." << endl; cerr << "\t(2) Ends will be set to the chromosome length if requested slop would" << endl; cerr << "\tforce it above the max chrom length." << endl; cerr << "\t(3) The genome file should tab delimited and structured as follows:" << endl; cerr << "\n\t" << endl << endl; cerr << "\tFor example, Human (hg19):" << endl; cerr << "\tchr1\t249250621" << endl; cerr << "\tchr2\t243199373" << endl; cerr << "\t..." << endl; cerr << "\tchr18_gl000207_random\t4262" << endl << endl; cerr << "Tips: " << endl; cerr << "\tOne can use the UCSC Genome Browser's MySQL database to extract" << endl; cerr << "\tchromosome sizes. For example, H. sapiens:" << endl << endl; cerr << "\tmysql --user=genome --host=genome-mysql.cse.ucsc.edu -A -e \\" << endl; cerr << "\t\"select chrom, size from hg19.chromInfo\" > hg19.genome" << endl << endl; // end the program here exit(1); }