Aligning contigs to reference... Aligning contigs to the reference Filtering alignments... Parsing coords... Loading reference... Loaded [meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa] Total Regions: 1 Total Region Length: 59999 Analyzing contigs... CONTIG: NODE_1_length_55421_cov_26.1146_ID_4744408 (54221bp) Best alignment score: 36102.0 (LEN: 36102, IDY: 100.00), Total number of alignments: 2 Looking for the best set of alignments (out of 2 total alignments) The best set is below. Score: 53970.5, number of alignments: 2, unaligned bases: 0 This contig is misassembled. Excluding internal overlap of size 1 between Alignment 1 and 2: 41879 59998 | 18120 1 | 18120 18120 --> 41880 59998 | 18119 1 | 18119 18119 Real Alignment 1: 41880 59998 | 18119 1 | 18119 18119 | 100.0 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_1_length_55421_cov_26.1146_ID_4744408 Extensive misassembly (relocation, inconsistency = 1200) between these two alignments Real Alignment 2: 4578 40679 | 54221 18120 | 36102 36102 | 100.0 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_1_length_55421_cov_26.1146_ID_4744408 CONTIG: NODE_7_length_3447_cov_44.4894_ID_2480336 (3447bp) Best alignment score: 3431.8 (LEN: 3447, IDY: 99.56), Total number of alignments: 1 One align captures most of this contig: 3 3449 | 3447 1 | 3447 3447 | 99.56 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_7_length_3447_cov_44.4894_ID_2480336 CONTIG: NODE_29_length_219_cov_25.5122_ID_8985538 (763bp) Best alignment score: 219.0 (LEN: 219, IDY: 100.00), Total number of alignments: 3 Looking for the best set of alignments (out of 3 total alignments) The best set is below. Score: 432.0, number of alignments: 3, unaligned bases: 211 This contig is misassembled. Real Alignment 1: 4394 4612 | 1 219 | 219 219 | 100.0 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Extensive misassembly (relocation, inconsistency = -1426) between these two alignments Real Alignment 2: 3395 3573 | 428 605 | 179 178 | 91.62 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Extensive misassembly (relocation, inconsistency = -1860) between these two alignments Real Alignment 3: 1717 1871 | 609 763 | 155 155 | 96.77 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Analyzing coverage... Writing SNPs into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/runs_per_reference/meta_ref_2/contigs_reports/minimap_output/meta_contigs_2.used_snps Analysis is finished! Founded SNPs were written into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/runs_per_reference/meta_ref_2/contigs_reports/minimap_output/meta_contigs_2.used_snps Results: Local Misassemblies: 0 Misassemblies: 3 Relocations: 3 Translocations: 0 Inversions: 0 Scaffold gap extensive misassemblies: 0 Scaffold gap local misassemblies: 0 Misassembled Contigs: 2 Misassembled Contig Bases: 54984 Misassemblies Inter-Contig Overlap ("Extra" Aligned Bases): 0 Unaligned Contigs: 0 + 0 part Half Unaligned Contigs with Misassemblies: 0 Unaligned Bases in Fully and Partially Unaligned Contigs: 0 Ambiguously Mapped Contigs: 0 Total Bases in Ambiguously Mapped Contigs: 0 "Extra" Aligned Bases in Ambiguously Mapped Contigs: 0 Note that --allow-ambiguity option was set to "one" and only first alignment per each of these contigs was used (there is no "extra" aligned bases). Covered Bases in Reference: 57976 Raw Aligned Bases in Assembly: 58220 Total Aligned Bases in Assembly (with "Extras"): 58220 SNPs: 34 Insertions: 0 Deletions: 1