# tfastx3 -T 32 -Q -p -b %NUMOFSCORES% -d 20 -m 0 -z 1 -E 1.0e-5 -m "F8C bio8144906429648998462.tmp.tsv" -m "F6 bio8144906429648998462.tmp.html" bio8144906429648998462.tmp @/home/psgendb/BIRCHDEV/dat/fasta/nt.nam 3
TFASTX compares a protein to a translated DNA data bank
version 36.3.8h Aug, 2019
Please cite:
Pearson et al, Genomics (1997) 46:24-36
Query: bio8144906429648998462.tmp
1>>>SRAAFP:CDS1 - 196 aa
Library: @/home/psgendb/BIRCHDEV/dat/fasta/nt.nam
242370672807 residues in 54289499 sequences
Statistics: Expectation_n fit: rho(ln(x))= 5.1512+/-0.00014; mu= 9.5732+/- 0.009
mean_var=71.5504+/-13.032, 0's: 13 Z-trim(112.4): 141 B-trim: 1825 in 2/94
Lambda= 0.151624
statistics sampled from 60000 (117185) to 8970211 sequences
Algorithm: TFASTX (3.8 June 2014) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -12/-2, shift: -20
ktup: 3, E-join: 0.5 (0.36), E-opt: 0.1 (0.0889), width: 16
Scan time: 12525.890
The best scores are: initn opt bits E(55167125)
gi|J02593.1|SRAAFP Sea raven (Hemitri ( 874) [f] 1369 1376 313.0 5.7e-82
dbj|AB283044.1| Brachyopsis rostratus ( 507) [f] 793 821 191.4 1.3e-45
gi|EU719616.1| Siniperca chuatsi anti ( 905) [f] 526 625 148.7 1.7e-32
ref|XM_028577120.1| PREDICTED: Perca (1140) [f] 400 518 125.4 2.2e-25
gi|FJ826540.1| Perca flavescens type (1218) [f] 400 518 125.4 2.3e-25
gi|M96154.1|OSEAFP Osmerus mordax ant ( 611) [f] 364 512 123.9 3.4e-25
gi|FJ826539.1| Perca flavescens type ( 859) [f] 404 502 121.8 2e-24
ref|XM_028577118.1| PREDICTED: Perca ( 927) [f] 404 502 121.8 2.1e-24
ref|XM_028577117.1| PREDICTED: Perca ( 834) [f] 382 500 121.3 2.7e-24
gi|DQ062448.1| Osmerus mordax clone 1 ( 539) [f] 377 496 120.3 3.5e-24
gi|FJ826541.1| Perca flavescens type ( 869) [f] 374 493 119.8 8e-24
ref|XM_018680358.1| PREDICTED: Lates ( 912) [f] 281 483 117.7 3.8e-23
ref|XM_018703530.1| PREDICTED: Lates ( 652) [f] 281 479 116.7 5.3e-23
ref|XM_018703522.1| PREDICTED: Lates ( 897) [f] 281 479 116.8 6.8e-23
ref|XM_028564253.1| PREDICTED: Perca ( 708) [f] 394 476 116.0 9e-23
ref|XM_003457801.5| PREDICTED: Oreoch (1273) [f] 203 479 116.9 9e-23
ref|XM_030429850.1| PREDICTED: Sparus ( 661) [f] 410 475 115.8 9.9e-23
ref|XM_025901702.1| PREDICTED: Oreoch (1726) [f] 249 479 117.0 1.1e-22
+- 127 342 87.0 1.2e-13
ref|XM_018680359.1| PREDICTED: Lates ( 856) [f] 281 474 115.7 1.4e-22
ref|XM_030754005.1| PREDICTED: Archoc ( 702) [f] 301 471 114.9 1.9e-22
ref|XM_013265793.3| PREDICTED: Oreoch (1365) [f] 414 474 115.8 2e-22
gi|EU660936.1| Dicentrarchus labrax C ( 719) [f] 373 470 114.7 2.3e-22
ref|XM_026162507.1| PREDICTED: Astato ( 812) [f] 402 470 114.8 2.5e-22
ref|XM_015021577.1| PREDICTED: Poecil ( 717) [f] 292 468 114.3 3e-22
ref|XM_030429846.1| PREDICTED: Sparus ( 888) [f] 266 468 114.4 3.6e-22
ref|XM_030429847.1| PREDICTED: Sparus ( 900) [f] 266 468 114.4 3.6e-22
ref|XM_030429845.1| PREDICTED: Sparus ( 945) [f] 266 468 114.4 3.8e-22
ref|XM_026162508.1| PREDICTED: Astato ( 891) [f] 397 466 113.9 4.9e-22
ref|XM_015021576.1| PREDICTED: Poecil ( 705) [f] 272 463 113.2 6.4e-22
ref|XM_015024499.1| PREDICTED: Poecil ( 648) [f] 188 462 113.0 7e-22
ref|XM_023399361.1| PREDICTED: Seriol ( 837) [f] 384 463 113.3 7.3e-22
ref|XM_005755315.1| PREDICTED: Pundam (1275) [f] 299 465 113.8 7.5e-22
ref|XM_026162511.1| PREDICTED: Astato ( 804) [f] 393 462 113.0 8.3e-22
ref|XM_026162509.1| PREDICTED: Astato ( 839) [f] 397 461 112.8 9.9e-22
ref|XM_014413924.2| PREDICTED: Maylan ( 815) [f] 391 460 112.6 1.1e-21
ref|XM_024800189.1| PREDICTED: Maylan ( 837) [f] 397 459 112.4 1.3e-21
ref|XM_026353424.1| PREDICTED: Anabas ( 483) [f] 354 455 111.3 1.6e-21
ref|XM_014976232.1| PREDICTED: Poecil ( 728) [f] 220 456 111.7 1.9e-21
ref|XM_012858718.2| PREDICTED: Fundul (1324) [f] 208 459 112.5 1.9e-21
ref|XM_022751368.1| PREDICTED: Seriol (1488) [f] 231 459 112.6 2.1e-21
ref|XM_024800191.1| PREDICTED: Maylan ( 823) [f] 397 454 111.3 2.8e-21
ref|XM_030162186.1| PREDICTED: Sphaer ( 570) [f] 111 452 110.7 2.9e-21
ref|XM_024800190.1| PREDICTED: Maylan ( 847) [f] 397 454 111.3 2.9e-21
ref|XM_030754008.1| PREDICTED: Archoc ( 575) [f] 304 451 110.5 3.4e-21
ref|XM_021314307.1| PREDICTED: Fundul (1325) [f] 208 455 111.6 3.5e-21
ref|XM_003457793.5| PREDICTED: Oreoch (1327) [f] 354 453 111.2 4.8e-21
ref|XM_018703542.1| PREDICTED: Lates ( 891) [f] 343 449 110.2 6.4e-21
ref|XM_018680360.1| PREDICTED: Lates ( 915) [f] 343 449 110.2 6.6e-21
ref|XM_012858321.2| PREDICTED: Fundul ( 774) [f] 222 447 109.7 7.8e-21
ref|XM_007542869.2| PREDICTED: Poecil ( 841) [f] 193 447 109.8 8.3e-21
>>>SRAAFP:CDS1, 196 aa vs @/home/psgendb/BIRCHDEV/dat/fasta/nt.nam library
>>gi|J02593.1|SRAAFP Sea raven (Hemitripterus (874 aa)
Frame: f initn: 1369 init1: 1369 opt: 1376 Z-score: 1643.0 bits: 313.0 E(55167125): 5.7e-82
Smith-Waterman score: 1376; 100.0% identity (100.0% similar) in 196 aa overlap (1-196:10-597)
10 20 30 40 50 60
SRAAFP MQRQQADTETREDISTAGLSIIFIVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTAT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
gi|J02 MQRQQADTETREDISTAGLSIIFIVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTAT
10 40 70 100 130 160
70 80 90 100 110 120
SRAAFP EAGPVSQRAPPNCPAGWQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFI
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
gi|J02 EAGPVSQRAPPNCPAGWQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFI
190 220 250 280 310 340
130 140 150 160 170 180
SRAAFP QTLNAGVVWIGGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDD
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
gi|J02 QTLNAGVVWIGGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDD
370 400 430 460 490 520
190
SRAAFP LPCPASHKSVCAMTF*
::::::::::::::::
gi|J02 LPCPASHKSVCAMTF*
550 580
>>dbj|AB283044.1| Brachyopsis rostratus mRN (507 aa)
Frame: f initn: 793 init1: 644 opt: 821 Z-score: 990.2 bits: 191.4 E(55167125): 1.3e-45
Smith-Waterman score: 821; 68.3% identity (84.1% similar) in 164 aa overlap (33-192:1-492)
40 50 60 70 80
SRAAFP MLTVSLLVCAMMALTQANDDKILKGTATEAGPVS----QRAPPNCPAGWQPLGDRCIYYE
:::::::::::::::::. : .::::::::: :: .: ::::: :.::.: :
dbj|AB MLTVSLLVCAMMALTQADHDGVLKGTATEAGEVSPVFRSRRALVCPAGWTLHGQRCFYSE
10 40 70 100 130 160
90 100 110 120 130 140
SRAAFP TTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNAGVVWIGGSACLQAGAWTWSDGTP
.::::: :::.::.. ::::::::: ::. .:. . ::.:::::::: ::::.:.::::
dbj|AB ATAMTWDLAEANCVNKGGHLASIHSLEEQLYIKDIVAGIVWIGGSACKVAGAWSWTDGTP
190 220 250 280 310 340
150 160 170 180 190
SRAAFP MNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCA
...:.:: :::.:.:. ::::::::.:.::::::::::: :.::
dbj|AB VDYRTWCPTKPNDILSDCCMQMTAAVDKCWDDLPCPASHASICA
370 400 430 460 490
>>gi|EU719616.1| Siniperca chuatsi antifree (905 aa)
Frame: f initn: 526 init1: 411 opt: 625 Z-score: 755.0 bits: 148.7 E(55167125): 1.7e-32
Smith-Waterman score: 625; 49.7% identity (73.2% similar) in 179 aa overlap (21-196:28-564)
30 40 50 60 70
SRAAFP IIFIVCTISTTRMLTVSLLVCAMMALTQAN--DDKILKGTATEAGPVS-QRAPPNCPAGW
::: . ::::::::::::::::::::.:: .: ..::.:. .. : .:: ::
gi|EU7 IIFNIYIISTTRMLTVSLLVCAMMALTRANGEEDTSINGTVTNDTNIDVLNARNGCPPGW
40 70 100 130 160 190
80 90 100 110 120 130
SRAAFP QPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNAGVVWIGGSACLQ
.:. ::. :. .::: :: ::. .::.::: ...:...::: ... ..::::::: .
gi|EU7 EPFDGRCFKLVTSRLTWAKAEKNCQAFGGNLASTRNSEDYNFIQQMTTELTWIGGSACQE
220 250 280 310 340 370
140 150 160 170 180 190
SRAAFP AGAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCAMTF*
..:: :::::::. ::. .:: .:: ::.:.... .:::: :: ::: :
gi|EU7 TNAWFWSDGTPMDKPLWCAGQPDGALAQCCLQINTGDGKCWDDQPCRNLLPSVCIKK**
400 430 460 490 520 550
>>ref|XM_028577120.1| PREDICTED: Perca fla (1140 aa)
Frame: f initn: 400 init1: 183 opt: 518 Z-score: 627.1 bits: 125.4 E(55167125): 2.2e-25
Smith-Waterman score: 518; 42.2% identity (68.3% similar) in 180 aa overlap (24-196:33-566)
30 40 50 60 70 80
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDR
:.: ::.:.::::::::::::::. :.: . .... .. : . : :::.:. .::
ref|XM IICIISATKMLTVSLLVCAMMALATADDVDVASNNTDVTS--SYEEGPACPASWHKYNDR
60 90 120 150 180
90 100 110 120 130
SRAAFP CIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWIGGSACLQ
:. : .. :. :: ::.. :.:::.:: ::..::: : ..::::.:: .
ref|XM CFLYVPRTVDWSDAEKNCQSSKGNLASVHSIEEYQFIQMIITQQTHANPMTWIGGTACQK
210 240 270 300 330 360
140 150 160 170 180 190
SRAAFP AGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKSVCAMTF*
. : :::: :..: ::. .:... . :..:. . .::::. : . :::: . :
ref|XM HSNWFWSDGRPFSFTFWCAGEPNNAGGNQGCLRMNYGEHNCWDDIQCSDKLPSVCARNP*
390 420 450 480 510 540
>>gi|FJ826540.1| Perca flavescens type II a (1218 aa)
Frame: f initn: 400 init1: 183 opt: 518 Z-score: 626.7 bits: 125.4 E(55167125): 2.3e-25
Smith-Waterman score: 518; 42.2% identity (68.3% similar) in 180 aa overlap (24-196:34-567)
30 40 50 60 70 80
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDR
:.: ::.:.::::::::::::::. :.: . .... .. : . : :::.:. .::
gi|FJ8 IICIISATKMLTVSLLVCAMMALATADDVDVASNNTDVTS--SYEEGPACPASWHKYNDR
40 70 100 130 160 190
90 100 110 120 130
SRAAFP CIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWIGGSACLQ
:. : .. :. :: ::.. :.:::.:: ::..::: : ..::::.:: .
gi|FJ8 CFLYVPRTVDWSDAEKNCQSSKGNLASVHSIEEYQFIQMIITQQTHANPMTWIGGTACQK
220 250 280 310 340 370
140 150 160 170 180 190
SRAAFP AGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKSVCAMTF*
. : :::: :..: ::. .:... . :..:. . .::::. : . :::: . :
gi|FJ8 HSNWFWSDGRPFSFTFWCAGEPNNAGGNQGCLRMNYGEHNCWDDIQCSDKLPSVCARNP*
400 430 460 490 520 550
>>gi|M96154.1|OSEAFP Osmerus mordax antifreeze (611 aa)
Frame: f initn: 364 init1: 274 opt: 512 Z-score: 623.8 bits: 123.9 E(55167125): 3.4e-25
Smith-Waterman score: 512; 43.7% identity (71.3% similar) in 174 aa overlap (22-192:30-542)
30 40 50 60 70 80
SRAAFP IFIVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLG
.. : .::::::: ..::::::.:::.: . : :. .: : . .::. :. ..
gi|M96 VINVKVISTTRML-AALLVCAMVALTRAANGDTGK-EAVMTGS-SGKNLTECPTDWKMFN
30 60 90 120 150 180
90 100 110 120 130
SRAAFP DRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLN-AGVV--WIGGSACLQA
::. .. . :: :. .::: :..:::::: ::..:.. :. ::.. ::::: : .
gi|M96 GRCFLFNPLQLHWAHAQISCMKDGANLASIHSLEEYAFVKELTTAGLIPAWIGGSDCHVS
210 240 270 300 330 360
140 150 160 170 180 190
SRAAFP GAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCA
: : :.: :.: .::...:: .:. ::.:..... .::.: :: : ::::
gi|M96 TYWFWMDSTSMDFTDWCAAQPDFTLTECCIQINVGVGKCWNDTPCTHLHASVCA
390 420 450 480 510 540
>>gi|FJ826539.1| Perca flavescens type II a (859 aa)
Frame: f initn: 404 init1: 178 opt: 502 Z-score: 609.9 bits: 121.8 E(55167125): 2e-24
Smith-Waterman score: 502; 41.5% identity (65.0% similar) in 183 aa overlap (24-196:33-581)
30 40 50 60 70 80
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATEAGP---VSQRAPPNCPAGWQPL
:.: ::::.::::::::::::::. :.: . ....: . : : :::.:
gi|FJ8 IICIISTTKMLTVSLLVCAMMALATADDADVTSSNSTSNASNNSSSTTEGPACPASWTKY
60 90 120 150 180 210
90 100 110 120 130
SRAAFP GDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWIGGSA
.:::. . .. :. :: ::.. :.:::.:: ::..::: : ..::::.
gi|FJ8 NDRCFLFVPRGLDWVDAEKNCQSSKGNLASVHSVEEYQFIQMIIKQQTHANPMTWIGGQD
240 270 300 330 360 390
140 150 160 170 180 190
SRAAFP CLQAGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKSVCAM
:. ..: :::: :. : ::. .:.. . :..:. . .::::. : . ::::
gi|FJ8 ALKNNVWFWSDGRPFYFTFWCAGEPNNGSGNQHCIEMNFGEHNCWDDVQCSIKLPSVCAR
420 450 480 510 540 570
SRAAFP TF*
. :
gi|FJ8 NP*
>>ref|XM_028577118.1| PREDICTED: Perca fla (927 aa)
Frame: f initn: 404 init1: 178 opt: 502 Z-score: 609.4 bits: 121.8 E(55167125): 2.1e-24
Smith-Waterman score: 502; 41.5% identity (65.0% similar) in 183 aa overlap (24-196:121-669)
30 40 50 60 70 80
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATEAGP---VSQRAPPNCPAGWQPL
:.: ::::.::::::::::::::. :.: . ....: . : : :::.:
ref|XM IICIISTTKMLTVSLLVCAMMALATADDADVTSSNSTSNASNNSSSTTEGPACPASWTKY
130 160 190 220 250 280
90 100 110 120 130
SRAAFP GDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWIGGSA
.:::. . .. :. :: ::.. :.:::.:: ::..::: : ..::::.
ref|XM NDRCFLFVPRGLDWVDAEKNCQSSKGNLASVHSVEEYQFIQMIIKQQTHANPMTWIGGQD
310 340 370 400 430 460
140 150 160 170 180 190
SRAAFP CLQAGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKSVCAM
:. ..: :::: :. : ::. .:.. . :..:. . .::::. : . ::::
ref|XM ALKNNVWFWSDGRPFYFTFWCAGEPNNGSGNQHCIEMNFGEHNCWDDVQCSIKLPSVCAR
490 520 550 580 610 640
SRAAFP TF*
. :
ref|XM NP*
>>ref|XM_028577117.1| PREDICTED: Perca fla (834 aa)
Frame: f initn: 382 init1: 189 opt: 500 Z-score: 607.7 bits: 121.3 E(55167125): 2.7e-24
Smith-Waterman score: 500; 40.1% identity (64.7% similar) in 187 aa overlap (24-196:39-599)
30 40 50 60 70
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATE-------AGPVSQRAPPNCPAG
:.: ::.:.::::::::::::::. :.: . ..... .. : : :::.
ref|XM IICIISATKMLTVSLLVCAMMALATADDAYVTSSNSSSNVSNNSSSNSSSYAEGPACPAS
60 90 120 150 180 210
80 90 100 110 120 130
SRAAFP WQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWI
:. .:::. . .. :. :: ::..: :.:::.:: ::..::: : ..::
ref|XM WHKYNDRCFLFIPRTLDWSEAEKNCQSLQGNLASVHSVEEYQFIQMIIKQQTHANPITWI
240 270 300 330 360 390
140 150 160 170 180
SRAAFP GGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKS
:: . ..: :::: :..: ::. .:.. . :.::. . .::::. : . :
ref|XM GGHDSPKNNVWFWSDGRPFSFTFWCAGEPNNGYGNQDCIQMNFGEHNCWDDVQCSIKLPS
420 450 480 510 540 570
190
SRAAFP VCAMTF*
::: . :
ref|XM VCARNP*
>>gi|DQ062448.1| Osmerus mordax clone 14 typ (539 aa)
Frame: f initn: 377 init1: 274 opt: 496 Z-score: 605.6 bits: 120.3 E(55167125): 3.5e-24
Smith-Waterman score: 496; 44.0% identity (70.5% similar) in 166 aa overlap (30-192:1-489)
30 40 50 60 70 80
SRAAFP TTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRCIYYET
::::: ..::::::.:::.: . : :. .: : . .::. :. .. :: ..
gi|DQ0 TTRML-AALLVCAMVALTRAANGDTGK-EAVMTGS-SGKNLTECPTDWKMFNGRCSLFNP
10 40 70 100 130 160
90 100 110 120 130 140
SRAAFP TAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLN-AGVV--WIGGSACLQAGAWTWSDG
. :: :. .::: :..:::::: ::..:.. :. ::.. ::::: : . : : :.
gi|DQ0 LQLHWAHAQISCMKDGANLASIHSLEEYAFVKELTTAGLIPAWIGGSDCHVSTYWFWMDS
190 220 250 280 310 340
150 160 170 180 190
SRAAFP TPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCA
: :.: .::...:: .:. ::.:..... .::.: :: : ::::
gi|DQ0 TSMDFTDWCAAQPDFTLTECCIQINVGVGKCWNDTPCTHLHASVCA
370 400 430 460
>>gi|FJ826541.1| Perca flavescens type II a (869 aa)
Frame: f initn: 374 init1: 181 opt: 493 Z-score: 599.2 bits: 119.8 E(55167125): 8e-24
Smith-Waterman score: 493; 39.6% identity (64.7% similar) in 187 aa overlap (24-196:34-594)
30 40 50 60 70
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATE-------AGPVSQRAPPNCPAG
:.: ::.:.::::::::::::::. :.: . ..... .. : : :::.
gi|FJ8 IICIISATKMLTVSLLVCAMMALATADDAYVTSSNSSSNVSNNSSSNSSSYAEGPACPAS
40 70 100 130 160 190
80 90 100 110 120 130
SRAAFP WQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWI
:. .:::. . .. :. :: ::.. :.:::.:: ::..::: : . ..::
gi|FJ8 WHKYNDRCFLFIPRTLDWSEAEKNCQSSKGNLASVHSVEEYQFIQMIIKQQTHGNPITWI
220 250 280 310 340 370
140 150 160 170 180
SRAAFP GGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKS
:: . ..: :::: :..: ::. .:.. . :.::. . .::::. : . :
gi|FJ8 GGHDSPKNNVWFWSDGRPFSFTFWCAGEPNNGYGNQDCIQMNFGEHNCWDDVQCSIKLPS
400 430 460 490 520 550
190
SRAAFP VCAMTF*
::: . :
gi|FJ8 VCARNP*
580
>>ref|XM_018680358.1| PREDICTED: Lates cal (912 aa)
Frame: f initn: 281 init1: 93 opt: 483 Z-score: 587.1 bits: 117.7 E(55167125): 3.8e-23
Smith-Waterman score: 483; 39.9% identity (65.6% similar) in 183 aa overlap (21-192:18-566)
30 40 50 60 70
SRAAFP IIFIVC-TISTTRMLTVSLLVCAMMALTQAN---DDKILKGTATEAGPVSQRAPPNCPAG
: ..: .. :..:::: .:::::::::.: ..: : . . .:. .: .:
ref|XM IYELLCLSFPTVKMLTVWVLVCAMMALTRAAALPEEKAKKDDQAVETDLVKRTYYGCSSG
30 60 90 120 150 180
80 90 100 110 120 130
SRAAFP WQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTL------NAGVVWI
:. .. ::... :::: :: :: ..::.:::.::..:. :: : . ..:.
ref|XM WSRFNRRCFHFVPKPMTWAQAERNCRSMGGNLASVHSMQEYHEIQRLIMTATYEYKAAWL
210 240 270 300 330 360
140 150 160 170 180
SRAAFP GGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVL-AACCMQMTAAADQCWDDLPCPASHKS
::: . ..: :::: :... .:: .:.. ::::. ::..::::: : : :
ref|XM GGSDAQEENVWLWSDGRPFHYSNWCHGEPNNYRRQQNCMQMNHAAQKCWDDLQCNAHLPS
390 420 450 480 510 540
190
SRAAFP VCA
.::
ref|XM ICA
>>ref|XM_018703530.1| PREDICTED: Lates cal (652 aa)
Frame: f initn: 281 init1: 93 opt: 479 Z-score: 584.4 bits: 116.7 E(55167125): 5.3e-23
Smith-Waterman score: 479; 41.0% identity (66.1% similar) in 183 aa overlap (21-192:3-548)
30 40 50 60 70
SRAAFP IIFIVC-TISTTRMLTVSLLVCAMMALTQA---NDDKILKGTATEAGPVSQRAPPNCPAG
: ..: .. :..:::: .:::::::::.: ..: : .:. : .:. .: .:
ref|XM IYELLCLSFPTVKMLTVWVLVCAMMALTRAVALPEEKAKKDDQAETDLV-KRTYYGCSSG
30 60 90 120 150
80 90 100 110 120 130
SRAAFP WQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTL------NAGVVWI
:. .. ::... :::: :: :: ..::.:::.:: .:. :: : . ..:.
ref|XM WSRFNRRCFHFVPKPMTWAQAERNCRSMGGNLASVHSVQEYHEIQRLIMTATYEYKAAWL
180 210 240 270 300 330
140 150 160 170 180
SRAAFP GGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVL-AACCMQMTAAADQCWDDLPCPASHKS
::: . ..: :::: :... .:: .:.. ::::. ::..::::: : : :
ref|XM GGSDAQEENVWLWSDGRPFHYSNWCHGEPNNYRRQQNCMQMNHAAQKCWDDLQCNAHLPS
360 390 420 450 480 510
190
SRAAFP VCA
.::
ref|XM ICA
540
>>ref|XM_018703522.1| PREDICTED: Lates cal (897 aa)
Frame: f initn: 281 init1: 93 opt: 479 Z-score: 582.4 bits: 116.8 E(55167125): 6.8e-23
Smith-Waterman score: 479; 39.9% identity (65.0% similar) in 183 aa overlap (21-192:3-551)
30 40 50 60 70
SRAAFP IIFIVC-TISTTRMLTVSLLVCAMMALTQA---NDDKILKGTATEAGPVSQRAPPNCPAG
: ..: .. :..:::: .:::::::::.: ..: : . . .:. .: .:
ref|XM IYELLCLSFPTVKMLTVWVLVCAMMALTRAVALPEEKAKKDDQAVETDLVKRTYYGCSSG
30 60 90 120 150 180
80 90 100 110 120 130
SRAAFP WQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTL------NAGVVWI
:. .. ::... :::: :: :: ..::.:::.:: .:. :: : . ..:.
ref|XM WSRFNRRCFHFVPKPMTWAQAERNCRSMGGNLASVHSVQEYHEIQRLIMTATYEYKAAWL
210 240 270 300 330 360
140 150 160 170 180
SRAAFP GGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVL-AACCMQMTAAADQCWDDLPCPASHKS
::: . ..: :::: :... .:: .:.. ::::. ::..::::: : : :
ref|XM GGSDAQEENVWLWSDGRPFHYSNWCHGEPNNYRRQQNCMQMNHAAQKCWDDLQCNAHLPS
390 420 450 480 510 540
190
SRAAFP VCA
.::
ref|XM ICA
>>ref|XM_028564253.1| PREDICTED: Perca fla (708 aa)
Frame: f initn: 394 init1: 197 opt: 476 Z-score: 580.3 bits: 116.0 E(55167125): 9e-23
Smith-Waterman score: 476; 44.3% identity (62.1% similar) in 174 aa overlap (26-192:1-486)
30 40 50 60 70 80
SRAAFP CTISTTRMLTVSLLVCAMMALTQA-NDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRC
: ::: ..: ::::::::::::.: . ..:.: ... ::.:: . ::
ref|XM CIISTMKVLIVSLLVCAMMALTRAAGKSHIVKRSSS------------CPSGWTGYNGRC
10 40 70 100 130
90 100 110 120 130
SRAAFP IYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNAGVV------WIGGSACLQA
. : :::::: :: ::. ::.:::.:: ::: ::.. .. :.::: :
ref|XM FLYVPTAMTWADAEKNCLYHGGNLASVHSFEEHHVIQSMILRITHMYPLTWLGGSDAQQE
160 190 220 250 280 310
140 150 160 170 180 190
SRAAFP GAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCA
:.: ::::::. : : .::. : :. :. . .. .:: :: ::::
ref|XM GSWFWSDGTPFRFNFWSPGQPDNRGNAHCLLMNFGDQNKFDDQPCSFRMPSVCA
340 370 400 430 460
>>ref|XM_003457801.5| PREDICTED: Oreochrom (1273 aa)
Frame: f initn: 203 init1: 203 opt: 479 Z-score: 580.3 bits: 116.9 E(55167125): 9e-23
Smith-Waterman score: 479; 38.1% identity (65.6% similar) in 189 aa overlap (14-196:232-792)
20 30 40 50 60 70
SRAAFP ISTAGLSIIFIVCTISTTRMLTVSLLVCAMMALTQAND--DKILKGTATEAGPVSQRAPP
..:. :... . .: : . ::.: . ::..:::.: . : .. : . . .::
ref|XM LQTVILGLVSQILNI-TMKKLTASAIFCAIIALTMAAEPRDGAEESLAPAKSHIVKRA-T
250 280 310 340 370 400
80 90 100 110 120
SRAAFP NCPAGWQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNAGV----
.:: :: .. ::. : ..:.:: :: ::. .:.::::.:: :. :: :.:
ref|XM DCPDGWTLISGRCFRYVPAVMNWANAEINCLYMGAHLASVHSWWEYHQIQRLTAPYGYRE
430 460 490 520 550 580
130 140 150 160 170 180
SRAAFP VWIGGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASH
.:.::. :.: ::::. ...: ::: .:.... :.::. . .::::: : .
ref|XM AWLGGTDAAYEGVWFWSDGSRFDYRRWCSGEPNNMFFQHCLQMNYRGPKCWDDLWCSYHR
610 640 670 700 730 760
190
SRAAFP KSVCAMTF*
::::: .:
ref|XM PSVCAMEI*
790
>>ref|XM_030429850.1| PREDICTED: Sparus au (661 aa)
Frame: f initn: 410 init1: 273 opt: 475 Z-score: 579.6 bits: 115.8 E(55167125): 9.9e-23
Smith-Waterman score: 475; 41.4% identity (65.5% similar) in 174 aa overlap (29-196:22-543)
30 40 50 60 70 80
SRAAFP STTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRCIYYE
:.:.:::::::::::::::.: . . ... :. . :.:: :: . ::. :
ref|XM SNTKMLTVSLLVCAMMALTRAAEGETDLNSGPEVTSSIAELMPSCPPGWTSYSIRCFLYV
40 70 100 130 160 190
90 100 110 120 130 140
SRAAFP TTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTL----NAG--VVWIGGSACLQAGAWT
..:::: :: :.. ::.:::.:: .:. :::. ..: ..:.:: : :.:
ref|XM PSTMTWANAEKYCQSQGGNLASVHSFDEQHVIQTMIQRQTSGYPLAWLGGCDAAQEGTWF
220 250 280 310 340 370
150 160 170 180 190
SRAAFP WSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCAMTF*
::::::... : . .::. : :. :. . .. .:: :: . ::: .:
ref|XM WSDGTPFSYNYWATGQPDNRANANCLLMNFGDEKKFDDQPCNYIKPFVCAKKL*
400 430 460 490 520
>>ref|XM_025901702.1| PREDICTED: Oreochrom (1726 aa)
Frame: f initn: 249 init1: 203 opt: 479 Z-score: 578.4 bits: 117.0 E(55167125): 1.1e-22
Smith-Waterman score: 479; 38.1% identity (65.6% similar) in 189 aa overlap (14-196:685-1245)
20 30 40 50 60 70
SRAAFP ISTAGLSIIFIVCTISTTRMLTVSLLVCAMMALTQAND--DKILKGTATEAGPVSQRAPP
..:. :... . .: : . ::.: . ::..:::.: . : .. : . . .::
ref|XM LQTVILGLVSQILNI-TMKKLTASAIFCAIIALTMAAEPRDGAEESLAPAKSHIVKRA-T
700 730 760 790 820 850
80 90 100 110 120
SRAAFP NCPAGWQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNAGV----
.:: :: .. ::. : ..:.:: :: ::. .:.::::.:: :. :: :.:
ref|XM DCPDGWTLISGRCFRYVPAVMNWANAEINCLYMGAHLASVHSWWEYHQIQRLTAPYGYRE
880 910 940 970 1000 1030
130 140 150 160 170 180
SRAAFP VWIGGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASH
.:.::. :.: ::::. ...: ::: .:.... :.::. . .::::: : .
ref|XM AWLGGTDAAYEGVWFWSDGSRFDYRRWCSGEPNNMFFQHCLQMNYRGPKCWDDLWCSYHR
1060 1090 1120 1150 1180 1210
190
SRAAFP KSVCAMTF*
::::: .:
ref|XM PSVCAMEI*
1240
Frame: f initn: 127 init1: 82 opt: 342 Z-score: 416.5 bits: 87.0 E(55167125): 1.2e-13
Smith-Waterman score: 342; 37.3% identity (62.7% similar) in 142 aa overlap (32-172:170-586)
40 50 60 70 80 90
SRAAFP RMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRCIYYETTA
..::. : :::::.: :. .. : . : . : :: :: .:.::. :. .
ref|XM KLLTLCALFCAMMAVTTAGGESHLFNWCIYC-P-DGWNQPRCPYGWTQVGSRCFIYNQNP
170 200 230 260 290 320
100 110 120 130 140 150
SRAAFP MTWALAETNCMKLGGHLASIHSQEEHS-FIQTLNAGVVWIGGSACLQAGAWTWSDGTPMN
:.: :. .: .::..:::.:. ::. .:: .. .::::: : : :.::: ..
ref|XM MSWDSAKRHCWELGANLASVHTYWEHQRIIQLFGNWPAWIGGSKG-PKGNWWWNDGTSFS
350 380 410 440 470 500
160 170
SRAAFP FRSWCSTKPDDVLAACCMQMTA
: :: .:.. :.::..
ref|XM FS*WCWGEPSNGYYENCLQMSS
530 560
>>ref|XM_018680359.1| PREDICTED: Lates cal (856 aa)
Frame: f initn: 281 init1: 93 opt: 474 Z-score: 576.8 bits: 115.7 E(55167125): 1.4e-22
Smith-Waterman score: 474; 42.1% identity (66.7% similar) in 171 aa overlap (32-192:1-510)
40 50 60 70 80
SRAAFP RMLTVSLLVCAMMALTQAN---DDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRCIYYE
.:::: .:::::::::.: ..: : .:. : .:. .: .::. .. ::...
ref|XM KMLTVWVLVCAMMALTRAAALPEEKAKKDDQAETDLV-KRTYYGCSSGWSRFNRRCFHFV
10 40 70 100 130 160
90 100 110 120 130 140
SRAAFP TTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTL------NAGVVWIGGSACLQAGAWT
:::: :: :: ..::.:::.::..:. :: : . ..:.::: . ..:
ref|XM PKPMTWAQAERNCRSMGGNLASVHSMQEYHEIQRLIMTATYEYKAAWLGGSDAQEENVWL
190 220 250 280 310 340
150 160 170 180 190
SRAAFP WSDGTPMNFRSWCSTKPDDVL-AACCMQMTAAADQCWDDLPCPASHKSVCA
:::: :... .:: .:.. ::::. ::..::::: : : :.::
ref|XM WSDGRPFHYSNWCHGEPNNYRRQQNCMQMNHAAQKCWDDLQCNAHLPSICA
370 400 430 460 490
>>ref|XM_030754005.1| PREDICTED: Archocent (702 aa)
Frame: f initn: 301 init1: 222 opt: 471 Z-score: 574.5 bits: 114.9 E(55167125): 1.9e-22
Smith-Waterman score: 471; 41.4% identity (66.9% similar) in 169 aa overlap (30-193:43-534)
30 40 50 60 70 80
SRAAFP TTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQ-RAPPNCPAGWQPLGDRCIYYE
: ..:.:: .::.:::: : ..: . :::. .. .:: :: ...::..:
ref|XM TMKLLSVSAHLCALMALTFA-----VEGDSDAEGPVTTVKSHSGCPRGWIEFNNRCFHYV
70 100 130 160 190
90 100 110 120 130 140
SRAAFP TTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNA--GV--VWIGGSACLQAGAWTWS
:: :::: :: ::. :::.:::.:.. :. :: :.: : .::::. .: .: ::
ref|XM TTRMTWANAEKNCLLLGGNLASVHNDMEYFEIQKLTATHGYRQAWIGGTDAVQKKVWFWS
220 250 280 310 340 370
150 160 170 180 190
SRAAFP DGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCAM
::::... .:: . .. :..:. .. .::::. : ::::.
ref|XM DGTPFHYSNWCPGESSNGRNDHCLRMNYSGAKCWDDVRCAIRLPSVCAI
400 430 460 490 520
196 residues in 1 query sequences
242370672807 residues in 54289499 library sequences
Tcomplib [36.3.8h Aug, 2019] (32 proc in memory [15G])
start: Mon Apr 20 14:58:45 2020 done: Mon Apr 20 15:27:46 2020
Total Scan time: 12525.890 Total Display time: 0.870
Function used was TFASTX [36.3.8h Aug, 2019]