# Copyright 2012 by Wibowo Arindrarto. All rights reserved. # This code is part of the Biopython distribution and governed by its # license. Please see the LICENSE file that should have been included # as part of this package. """Tests for online Entrez access. This file include tests for accessing the online Entrez service and parsing the returned results. Note that we are merely testing the access and whether the results are parseable. Detailed tests on each Entrez service are not within the scope of this file as they are already covered in test_Entrez.py. """ import os import unittest import requires_internet requires_internet.check() from Bio import Entrez from Bio import Medline from Bio import SeqIO from Bio.SeqRecord import SeqRecord if os.name == 'java': try: from xml.parsers.expat import XML_PARAM_ENTITY_PARSING_ALWAYS del XML_PARAM_ENTITY_PARSING_ALWAYS except ImportError: from Bio import MissingPythonDependencyError raise MissingPythonDependencyError("The Bio.Entrez XML parser fails on " "Jython, see http://bugs.jython.org/issue1447") # This lets us set the email address to be sent to NCBI Entrez: Entrez.email = "biopython-dev@biopython.org" URL_HEAD = "https://eutils.ncbi.nlm.nih.gov/entrez/eutils/" URL_TOOL = "tool=biopython" URL_EMAIL = "email=biopython-dev%40biopython.org" class EntrezOnlineCase(unittest.TestCase): def test_read_from_url(self): """Test Entrez.read from URL""" handle = Entrez.einfo() self.assertTrue(handle.url.startswith(URL_HEAD + "einfo.fcgi?"), handle.url) self.assertTrue(URL_TOOL in handle.url) self.assertTrue(URL_EMAIL in handle.url) rec = Entrez.read(handle) handle.close() self.assertTrue(isinstance(rec, dict)) self.assertTrue('DbList' in rec) # arbitrary number, just to make sure that DbList has contents self.assertTrue(len(rec['DbList']) > 5) def test_parse_from_url(self): """Test Entrez.parse from URL""" handle = Entrez.efetch(db='protein', id='15718680,157427902,119703751', retmode='xml') self.assertTrue(handle.url.startswith(URL_HEAD + "efetch.fcgi?"), handle.url) self.assertTrue(URL_TOOL in handle.url) self.assertTrue(URL_EMAIL in handle.url) self.assertTrue("id=15718680%2C157427902%2C119703751" in handle.url, handle.url) recs = list(Entrez.parse(handle)) handle.close() self.assertEqual(3, len(recs)) # arbitrary number, just to make sure the parser works self.assertTrue(all(len(rec).keys > 5) for rec in recs) def test_webenv_search(self): """Test Entrez.search from link webenv history""" handle = Entrez.elink(db='nucleotide', dbfrom='protein', id='22347800,48526535', webenv=None, query_key=None, cmd='neighbor_history') self.assertTrue(handle.url.startswith(URL_HEAD + "elink.fcgi?"), handle.url) self.assertTrue(URL_TOOL in handle.url) self.assertTrue(URL_EMAIL in handle.url) self.assertTrue("id=22347800%2C48526535" in handle.url, handle.url) recs = Entrez.read(handle) handle.close() record = recs.pop() webenv = record['WebEnv'] query_key = record['LinkSetDbHistory'][0]['QueryKey'] handle = Entrez.esearch(db='nucleotide', term=None, retstart=0, retmax=10, webenv=webenv, query_key=query_key, usehistory='y') self.assertTrue(handle.url.startswith(URL_HEAD + "esearch.fcgi?"), handle.url) self.assertTrue(URL_TOOL in handle.url) self.assertTrue(URL_EMAIL in handle.url) search_record = Entrez.read(handle) handle.close() self.assertEqual(2, len(search_record['IdList'])) def test_seqio_from_url(self): """Test Entrez into SeqIO.read from URL""" handle = Entrez.efetch(db='nucleotide', id='186972394', rettype='gb', retmode='text') self.assertTrue(handle.url.startswith(URL_HEAD + "efetch.fcgi?"), handle.url) self.assertTrue(URL_TOOL in handle.url) self.assertTrue(URL_EMAIL in handle.url) self.assertTrue("id=186972394" in handle.url) record = SeqIO.read(handle, 'genbank') handle.close() self.assertTrue(isinstance(record, SeqRecord)) self.assertEqual('EU490707.1', record.id) self.assertEqual(1302, len(record)) def test_medline_from_url(self): """Test Entrez into Medline.read from URL""" handle = Entrez.efetch(db="pubmed", id='19304878', rettype="medline", retmode="text") self.assertTrue(handle.url.startswith(URL_HEAD + "efetch.fcgi?"), handle.url) self.assertTrue(URL_TOOL in handle.url) self.assertTrue(URL_EMAIL in handle.url) self.assertTrue("id=19304878" in handle.url) record = Medline.read(handle) handle.close() self.assertTrue(isinstance(record, dict)) self.assertEqual('19304878', record['PMID']) self.assertEqual('10.1093/bioinformatics/btp163 [doi]', record['LID']) def test_efetch_biosystems_xml(self): """Test Entrez parser with XML from biosystems""" handle = Entrez.efetch(id="1134002", db="biosystems", retmode="xml") records = list(Entrez.parse(handle)) handle.close() self.assertEqual(len(records), 1) self.assertEqual(records[0]['System_sysid']['Sys-id']['Sys-id_bsid'], '1134002') def test_efetch_taxonomy_xml(self): """Test Entrez using a integer id - like a taxon id""" handle = Entrez.efetch(db="taxonomy", id=3702, retmode="XML") taxon_record = Entrez.read(handle) self.assertTrue(1, len(taxon_record)) self.assertTrue('TaxId' in taxon_record[0]) self.assertTrue('3702', taxon_record[0]['TaxId']) def test_elink(self): # Commas: Link from protein to gene handle = Entrez.elink(db="gene", dbfrom="protein", id="15718680,157427902,119703751") self.assertTrue(handle.url.startswith(URL_HEAD + "elink.fcgi"), handle.url) self.assertTrue(URL_TOOL in handle.url) self.assertTrue(URL_EMAIL in handle.url) self.assertTrue("id=15718680%2C157427902%2C119703751" in handle.url, handle.url) handle.close() # Multiple ID entries: Find one-to-one links from protein to gene handle = Entrez.elink(db="gene", dbfrom="protein", id=["15718680", "157427902", "119703751"]) self.assertTrue(handle.url.startswith(URL_HEAD + "elink.fcgi"), handle.url) self.assertTrue(URL_TOOL in handle.url) self.assertTrue(URL_EMAIL in handle.url) self.assertTrue("id=15718680" in handle.url, handle.url) self.assertTrue("id=157427902" in handle.url, handle.url) self.assertTrue("id=119703751" in handle.url, handle.url) handle.close() def test_epost(self): handle = Entrez.epost("nuccore", id="186972394,160418") self.assertEqual(URL_HEAD + "epost.fcgi", handle.url) handle.close() handle = Entrez.epost("nuccore", id=["160418", "160351"]) self.assertEqual(URL_HEAD + "epost.fcgi", handle.url) handle.close() def test_egquery(self): handle = Entrez.egquery(term="biopython") record = Entrez.read(handle) handle.close() done = False for row in record["eGQueryResult"]: if "pmc" in row["DbName"]: self.assertTrue(int(row["Count"]) > 60) done = True self.assertTrue(done) def test_espell(self): handle = Entrez.espell(term="biopythooon") record = Entrez.read(handle) handle.close() self.assertEqual(record["Query"], "biopythooon") self.assertEqual(record["CorrectedQuery"], "biopython") def test_ecitmatch(self): citation = { "journal_title": "proc natl acad sci u s a", "year": "1991", "volume": "88", "first_page": "3248", "author_name": "mann bj", "key": "citation_1" } handle = Entrez.ecitmatch(db="pubmed", bdata=[citation]) self.assertTrue("retmode=xml" in handle.url, handle.url) result = handle.read() expected_result = "proc natl acad sci u s a|1991|88|3248|mann bj|citation_1|2014248\n" self.assertEquals(result, expected_result) def test_fetch_xml_schemas(self): handle = Entrez.efetch("protein", id="783730874", rettype="ipg", retmode="xml") records = list(Entrez.parse(handle)) handle.close() self.assertEqual(len(records), 1) self.assertTrue("Product" in records[0]) self.assertTrue("Statistics" in records[0]) self.assertTrue("RedundantGiList" in records[0]) if __name__ == "__main__": runner = unittest.TextTestRunner(verbosity=2) unittest.main(testRunner=runner)