LOCUS NZ_KB731323 3419 bp DNA linear CON 17-JAN-2015 DEFINITION Thermoanaerobacter thermohydrosulfuricus WC1 scaffold00062, whole genome shotgun sequence. ACCESSION NZ_KB731323 NZ_AMYG01000000 VERSION NZ_KB731323.1 GI:490541858 DBLINK BioProject: PRJNA224116 BioSample: SAMN02471871 KEYWORDS WGS; RefSeq. SOURCE Thermoanaerobacter thermohydrosulfuricus WC1 ORGANISM Thermoanaerobacter thermohydrosulfuricus WC1 Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Thermoanaerobacter. REFERENCE 1 (bases 1 to 3419) AUTHORS Verbeke,T.J., Zhang,X., Henrissat,B., Spicer,V., Rydzak,T., Krokhin,O.V., Fristensky,B., Levin,D.B. and Sparling,R. TITLE Genomic evaluation of Thermoanaerobacter spp. for the construction of designer co-cultures to improve lignocellulosic biofuel production JOURNAL PLoS ONE 8 (3), E59362 (2013) PUBMED 23555660 REFERENCE 2 (bases 1 to 3419) AUTHORS Verbeke,T.J., Zhang,X., Spicer,V., Rydzak,T., Krokhin,O.V., Fristensky,B., Levin,D.B. and Sparling,R. TITLE Direct Submission JOURNAL Submitted (09-OCT-2012) Department of Microbiology, University of Manitoba, 66 Chancellors Circle, Winnipeg, MB R3T 2N2, Canada COMMENT VALIDATED REFSEQ: This record has undergone validation or preliminary review. Bacteria available from Richard Sparling, Department of Microbiology, University of Manitoba, 66 Chancellors Circle, Winnipeg, MB, R3T 2N2, Canada. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Method :: Newbler v. 2.6 Assembly Name :: TthWC1 draft genome1 Long Assembly Name :: Thermoanaerobacter thermohydrosulfuricus WC1 draft genome Genome Coverage :: 56.0x Sequencing Technology :: 454; Illumina HiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 12/22/2014 21:18:45 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 2.9 (rev. 454985) Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes :: 2,587 CDS :: 2,482 Pseudo Genes :: 46 CRISPR Arrays :: 2 rRNAs :: 3 ( 5S, 16S, 23S ) tRNAs :: 55 ncRNA :: 1 Frameshifted Genes :: 39 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..3419 /organism="Thermoanaerobacter thermohydrosulfuricus WC1" /mol_type="genomic DNA" /strain="WC1" /isolation_source="woodchip compost" /db_xref="taxon:1198630" /country="Canada" /collection_date="Jul-2006" gene <1..157 /locus_tag="TTHWC1_RS12915" /old_locus_tag="TthWC1_2680" CDS <1..157 /locus_tag="TTHWC1_RS12915" /old_locus_tag="TthWC1_2680" /inference="EXISTENCE: similar to AA sequence:RefSeq:WP_003871786.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=2 /transl_table=11 /product="S-layer domain containing protein" /protein_id="WP_004399092.1" /db_xref="GI:490533920" /translation="ISEWAKNVVANAVKLGIVRGYEDNTFKPKDNATRAEAAAMLYRI LEKTGNI" gene complement(194..1087) /locus_tag="TTHWC1_RS12920" /old_locus_tag="TthWC1_2681" CDS complement(194..1087) /locus_tag="TTHWC1_RS12920" /old_locus_tag="TthWC1_2681" /inference="EXISTENCE: similar to AA sequence:RefSeq:WP_003871271.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="ATPase" /protein_id="WP_003871271.1" /db_xref="GI:489968027" /translation="MKCTVCRKPAIVKFPAHNSAFCEEHLDAFFMRQVAKTIEKYKML PPKGRVVVAISGGKDSLVTTFVLKRLGYEVLGFFIDLGIEENNFSSRSREVVENFCKE NDIPLEIVSLKEKFRKGIPDVAKHQDRICALCGVTKRHLMNEYTLSVKADALATGHTL DDMAKLLLANLFRWDLHHLSKGTPVLPEEPGFARKIKPLAFQAEEEIIAFAKLHNIKP VTAVCPYSREAKYTRYQEALDMLEENSPGIKRSFYKNYTKYAHLFAGTSARPPKINCE VCGFPSVSPVCTFCRTWVKTD" gene 1265..2107 /locus_tag="TTHWC1_RS12925" /old_locus_tag="TthWC1_2682" CDS 1265..2107 /locus_tag="TTHWC1_RS12925" /old_locus_tag="TthWC1_2682" /EC_number="4.2.1.2" /inference="EXISTENCE: similar to AA sequence:RefSeq:WP_004406979.1" /note="Catalyzes the reversible hydration of fumaric acid to yield I-malic acid; Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hydro-lyase, Fe-S type, tartrate/fumarate subfamily, alpha region" /protein_id="WP_004406979.1" /db_xref="GI:490541854" /translation="MKVIEAKKITEIVKLLCIEANYELPQDIFESLKERIKEETSPLG REILKDIVANAEIAKTQRMPICQDTGIVVVFVEIGQEVHIVNGNLEEAINEGVRLGYE EGYLRKSVVKSPILRIITGDNTPAIIHYHICEGNRFSITVMPKGAGSENMSALKMLKP SDGVEGIKNFVIETVEKAGPNACPPSIVGIGIGGDFELAPYLAKKALLRKVGERNTDE VIAQLEEELLQEINTLGVGPQGLGGSTTALDVHIETYPTHIASLPVAVNLGCHVTRHA TFVL" gene 2128..2688 /locus_tag="TTHWC1_RS12930" /old_locus_tag="TthWC1_2683" CDS 2128..2688 /locus_tag="TTHWC1_RS12930" /old_locus_tag="TthWC1_2683" /EC_number="4.2.1.2" /inference="EXISTENCE: similar to AA sequence:RefSeq:WP_006569883.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta region" /protein_id="WP_004406981.1" /db_xref="GI:490541856" /translation="MYKKVTTPLDEGIVNQLKAGDLILLSGKIYTARDEAHKRMIESL NRGEKLPFEIRNSVIYYVGPCPPKPGQVVGSCGPTTSGRMDKYTPVLIELGLKGMIGK GYRSKEVIEAMKKYKAVYFTAIGGAGALLAQKVKKAEIVAYEDLGTEAIYKFLVEDLP VIVTIDIYGNNLYEIEREKYKTNLKR" gene 2694..3254 /locus_tag="TTHWC1_RS12935" /old_locus_tag="TthWC1_2684" CDS 2694..3254 /locus_tag="TTHWC1_RS12935" /old_locus_tag="TthWC1_2684" /inference="EXISTENCE: similar to AA sequence:RefSeq:WP_003871274.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DNA polymerase" /protein_id="WP_003871274.1" /db_xref="GI:489968030" /translation="MALLPLKELYLECLHCTKCDLSKTKTNMVFGEGNLRAKVMFVGE GPGRDEDLQGRPFVGRAGQLLNKMLEEVGLKREEVYIANVVKCRPPNNRVPLQSEIDA CLPYLRNQVAIIAPKIIVCLGATAAKAIIDKNFKITTMRGQWFERKGVKIIATYHPAA ILRDPEKLEPAMEDFKKIKEELNKLL" CONTIG join(AMYG01000077.1:1..3419) //