#include #include #include #include #include #include "bwamem.h" #include "kseq.h" // for the FASTA/Q parser KSEQ_DECLARE(gzFile) int main(int argc, char *argv[]) { bwaidx_t *idx; gzFile fp; kseq_t *ks; mem_opt_t *opt; if (argc < 3) { fprintf(stderr, "Usage: bwamem-lite \n"); return 1; } idx = bwa_idx_load(argv[1], BWA_IDX_ALL); // load the BWA index if (NULL == idx) { fprintf(stderr, "Index load failed.\n"); exit(EXIT_FAILURE); } fp = strcmp(argv[2], "-")? gzopen(argv[2], "r") : gzdopen(fileno(stdin), "r"); if (NULL == fp) { fprintf(stderr, "Couldn't open %s : %s\n", strcmp(argv[2], "-") ? argv[2] : "stdin", errno ? strerror(errno) : "Out of memory"); exit(EXIT_FAILURE); } ks = kseq_init(fp); // initialize the FASTA/Q parser opt = mem_opt_init(); // initialize the BWA-MEM parameters to the default values while (kseq_read(ks) >= 0) { // read one sequence mem_alnreg_v ar; int i, k; ar = mem_align1(opt, idx->bwt, idx->bns, idx->pac, ks->seq.l, ks->seq.s); // get all the hits for (i = 0; i < ar.n; ++i) { // traverse each hit mem_aln_t a; if (ar.a[i].secondary >= 0) continue; // skip secondary alignments a = mem_reg2aln(opt, idx->bns, idx->pac, ks->seq.l, ks->seq.s, &ar.a[i]); // get forward-strand position and CIGAR // print alignment printf("%s\t%c\t%s\t%ld\t%d\t", ks->name.s, "+-"[a.is_rev], idx->bns->anns[a.rid].name, (long)a.pos, a.mapq); for (k = 0; k < a.n_cigar; ++k) // print CIGAR printf("%d%c", a.cigar[k]>>4, "MIDSH"[a.cigar[k]&0xf]); printf("\t%d\n", a.NM); // print edit distance free(a.cigar); // don't forget to deallocate CIGAR } free(ar.a); // and deallocate the hit list } free(opt); kseq_destroy(ks); gzclose(fp); bwa_idx_destroy(idx); return 0; }