##gff-version 3 #Quantitative Data #GBrowse can plot quantitative data such as alignment scores, #confidence scores from gene prediction programs, and microarray #intensity data. There is a simple format that can be placed directly #inside of a GFF3 file but does not scale to very large data sets, and #a "WIG" format designed for very high-density quantitative data such #as tiling arrays. #We first look at the simple format: ctg123 affy microarray_oligo 1 100 281 . . Name=Expt1 ctg123 affy microarray_oligo 101 200 183 . . Name=Expt1 ctg123 affy microarray_oligo 201 300 213 . . Name=Expt1 ctg123 affy microarray_oligo 301 400 191 . . Name=Expt1 ctg123 affy microarray_oligo 401 500 288 . . Name=Expt1 ctg123 affy microarray_oligo 501 600 184 . . Name=Expt1 #In this format, which can be embedded directly in the GFF3 file, each #data point is a distinct feature with a start and end point. The #features are grouped together by giving them a common experimental #name so that they can be retrieved together. We use the score field #(column 6) to represent the quantitative information #(e.g. hybridization intensity). #In contrast, when using WIG format, the quantitative data is kept #outside of the main database in a special-purpose binary file that is #kept somewhere on the file system. In this case the GFF3 file #contains a single line per experiment like this one: #ctg123 . microarray_oligo 1 50000 . . . Name=example;wigfile=/usr/data/ctg123.Expt1.wig #The .wig file is created and managed using a script called #wiggle2gff3.pl that comes with GBrowse. Instructions on how to use #this script is described in the GBrowse Administration Tutorial.