/***************************************************************************** # Copyright (C) 1994-2008 by David Gordon. # All rights reserved. # # This software is part of a beta-test version of the Consed/Autofinish # package. It should not be redistributed or # used for any commercial purpose, including commercially funded # sequencing, without written permission from the author and the # University of Washington. # # This software is provided ``AS IS'' and any express or implied # warranties, including, but not limited to, the implied warranties of # merchantability and fitness for a particular purpose, are disclaimed. # In no event shall the authors or the University of Washington be # liable for any direct, indirect, incidental, special, exemplary, or # consequential damages (including, but not limited to, procurement of # substitute goods or services; loss of use, data, or profits; or # business interruption) however caused and on any theory of liability, # whether in contract, strict liability, or tort (including negligence # or otherwise) arising in any way out of the use of this software, even # if advised of the possibility of such damage. # # Building Consed from source is error prone and not simple which is # why I provide executables. Due to time limitations I cannot # provide any assistance in building Consed. Even if you do not # modify the source, you may introduce errors due to using a # different version of the compiler, a different version of motif, # different versions of other libraries than I used, etc. For this # reason, if you discover Consed bugs, I can only offer help with # those bugs if you first reproduce those bugs with an executable # provided by me--not an executable you have built. # # Modifying Consed is also difficult. Although Consed is modular, # some modules are used by many other modules. Thus making a change # in one place can have unforeseen effects on many other features. # It may takes months for you to notice these other side-effects # which may not seen connected at all. It is not feasable for me to # provide help with modifying Consed sources because of the # potentially huge amount of time involved. # #*****************************************************************************/ #include "primerStickiestFalseMatch.h" #include "rwcstring.h" #include "primerType.h" #include "consedParameters.h" #include "contig.h" #include "abs.h" void primerStickiestFalseMatch( primerType* pPrimer, const int nPrimerNumber, RWCString& soBigOutput ) { soBigOutput = ""; soBigOutput.increaseMaxLength( (size_t) 2000 ); char szDescription[500]; int n5PrimeFalseMatch = 0; int n3PrimeFalseMatch = 0; RWCString soFalseMatchContigName; // handle case in which there is no false match at all // so pContigOfStickiestFalseMatch is NULL if ( !pPrimer->pContigOfStickiestFalseMatch_ ) { soBigOutput.increaseMaxLengthIfNecessary( 200 ); soBigOutput.appendFormat( "no false match. nScoreOfStickiestFalseMatch_ = %d\n", pPrimer->nScoreOfStickiestFalseMatch_ ); return; } if ( pPrimer->bContigOfStickiestFalseMatchIsComplemented_ ) { n5PrimeFalseMatch = pPrimer->pContigOfStickiestFalseMatch_->nComplementUnpaddedIndex( pPrimer->nUnpaddedOfStickiestFalseMatch_ ); n3PrimeFalseMatch = n5PrimeFalseMatch - consedParameters::pGetConsedParameters()->nPrimersMinimumLengthOfAPrimerToUse_ + 1; } else { n5PrimeFalseMatch = pPrimer->nUnpaddedOfStickiestFalseMatch_; n3PrimeFalseMatch = n5PrimeFalseMatch + consedParameters::pGetConsedParameters()->nPrimersMinimumLengthOfAPrimerToUse_ - 1; } soFalseMatchContigName = pPrimer->pContigOfStickiestFalseMatch_->soGetName(); for( int n = 0; n < pPrimer->nUnpaddedLength_; ++n ) soBigOutput.append( *(pPrimer->szPrimer_ + n) ); soBigOutput.increaseMaxLengthIfNecessary( 200 ); soBigOutput.appendFormat( " primer: %d match elsewhere score: %d\n", nPrimerNumber, pPrimer->nScoreOfStickiestFalseMatch_ ); // false matches are determined by examining just the suboligo of // length nPrimersMinimumLengthOfAPrimer_ // This may be less than nUnpaddedLength_ of the primer. So to // show the alignment, put spaces to start with if ( ABS( n5PrimeFalseMatch - n3PrimeFalseMatch ) + 1 < pPrimer->nUnpaddedLength_ ) { int nNumberOfSpacesToAdd = pPrimer->nUnpaddedLength_ - ABS( n5PrimeFalseMatch - n3PrimeFalseMatch ) - 1; for( int n = 0; n < nNumberOfSpacesToAdd; ++n ) soBigOutput.append( ' ' ); } if ( pPrimer->pContigOfStickiestFalseMatch_ ) { if ( pPrimer->bContigOfStickiestFalseMatchIsComplemented_ ) { for( int nUnpadded = n5PrimeFalseMatch; nUnpadded >= n3PrimeFalseMatch; --nUnpadded ) { int nConsPos =pPrimer->pContigOfStickiestFalseMatch_->nPaddedIndexFast( nUnpadded ); char c = pPrimer->pContigOfStickiestFalseMatch_->ntGetCons( nConsPos ).cGetBase(); soBigOutput.append( cComplementBase( c ) ); } } else { for( int nUnpadded = n5PrimeFalseMatch; nUnpadded <= n3PrimeFalseMatch; ++nUnpadded ) { int nConsPos = pPrimer->pContigOfStickiestFalseMatch_->nPaddedIndexFast( nUnpadded ); char c = pPrimer->pContigOfStickiestFalseMatch_->ntGetCons( nConsPos ).cGetBase(); soBigOutput.append( c ); } } } soBigOutput.increaseMaxLengthIfNecessary( 1000 ); soBigOutput.appendFormat( " false match at %s 5': %6d 3': %6d %s\n", (char*) soFalseMatchContigName.data(), n5PrimeFalseMatch, n3PrimeFalseMatch, ( pPrimer->bContigOfStickiestFalseMatchIsComplemented_ ? "Complemented" : "not complemented" ) ); }