LOCUS CVU55762 4733 bp DNA circular SYN 22-AUG-2003 DEFINITION Cloning vector pEGFP-N1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds. ACCESSION U55762 VERSION U55762.1 GI:1377911 KEYWORDS . SOURCE Cloning vector pEGFP-N1 ORGANISM Cloning vector pEGFP-N1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4733) AUTHORS Cormack,B.P., Valdivia,R.H. and Falkow,S. TITLE FACS-optimized mutants of the green fluorescent protein (GFP) JOURNAL Gene 173 (1 SPEC NO), 33-38 (1996) PUBMED 8707053 REFERENCE 2 (bases 1 to 4733) AUTHORS Kitts,P.A. TITLE pEGFP-N1 complete sequence JOURNAL Unpublished REFERENCE 3 (bases 1 to 4733) AUTHORS Kitts,P.A. TITLE Direct Submission JOURNAL Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order call (415) 424-8222 or (800) 662-2566, extension 1. International customers, please contact your local distributor. For technical information, call (415) 424-8222 or (800) 662-2566, extension 3. This sequence has been compiled from information in the sequence databases, published literature and other sources, together with partial sequences obtained by CLONTECH. If you suspect there is an error in this sequence, please contact CLONTECH's Technical Service Department at (415) 424-8222 or (800) 662-2566, extension 3 or E-mail TECH@CLONTECH.COM. FEATURES Location/Qualifiers source 1..4733 /organism="Cloning vector pEGFP-N1" /mol_type="genomic DNA" /db_xref="taxon:47945" gene 679..1398 /gene="egfp" CDS 679..1398 /gene="egfp" /codon_start=1 /transl_table=11 /product="enhanced green fluorescent protein" /protein_id="AAB02574.1" /db_xref="GI:1377912" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" CDS 2629..3423 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAB02575.1" /db_xref="GI:1377913" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 661 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 781 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 841 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 961 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 1021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 1081 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 1141 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 1201 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 1261 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 1321 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 1381 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg 1441 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 1501 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 1561 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 1621 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc 1681 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc 1741 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag 1801 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 1861 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa 1921 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 1981 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 2041 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc 2101 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 2161 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 2221 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt 2281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2341 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2401 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 2461 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 2521 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 2581 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa 2641 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg 2701 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc 2761 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca 2821 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc 2881 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca 2941 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat 3001 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca 3061 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg 3121 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc 3181 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct 3241 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct 3301 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac 3361 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc 3421 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 3481 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 3541 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg 3601 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa 3661 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt 3721 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 3781 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca 3841 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt 3901 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 3961 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 4021 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 4081 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 4141 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 4201 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 4261 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 4321 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 4381 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 4441 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 4501 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 4561 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 4621 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 4681 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat //