# Copyright 2007-2016 by Peter Cock. All rights reserved. # # This file is part of the Biopython distribution and governed by your # choice of the "Biopython License Agreement" or the "BSD 3-Clause License". # Please see the LICENSE file that should have been included as part of this # package. """Bio.SeqIO support for the "genbank" and "embl" file formats. You are expected to use this module via the Bio.SeqIO functions. Note that internally this module calls Bio.GenBank to do the actual parsing of GenBank, EMBL and IMGT files. See Also: International Nucleotide Sequence Database Collaboration http://www.insdc.org/ GenBank http://www.ncbi.nlm.nih.gov/Genbank/ EMBL Nucleotide Sequence Database http://www.ebi.ac.uk/embl/ DDBJ (DNA Data Bank of Japan) http://www.ddbj.nig.ac.jp/ IMGT (use a variant of EMBL format with longer feature indents) http://imgt.cines.fr/download/LIGM-DB/userman_doc.html http://imgt.cines.fr/download/LIGM-DB/ftable_doc.html http://www.ebi.ac.uk/imgt/hla/docs/manual.html """ from __future__ import print_function import warnings from datetime import datetime from Bio import BiopythonWarning from Bio.Seq import UnknownSeq from Bio.GenBank.Scanner import GenBankScanner, EmblScanner, _ImgtScanner from Bio import Alphabet from Bio.SeqIO.Interfaces import SequentialSequenceWriter from Bio import SeqFeature from Bio._py3k import _is_int_or_long from Bio._py3k import basestring # NOTE # ==== # The "brains" for parsing GenBank, EMBL and IMGT files (and any # other flat file variants from the INSDC in future) is in # Bio.GenBank.Scanner (plus the _FeatureConsumer in Bio.GenBank) # However, all the writing code is in this file. def GenBankIterator(handle): """Break up a Genbank file into SeqRecord objects. Every section from the LOCUS line to the terminating // becomes a single SeqRecord with associated annotation and features. Note that for genomes or chromosomes, there is typically only one record. This gets called internally by Bio.SeqIO for the GenBank file format: >>> from Bio import SeqIO >>> for record in SeqIO.parse("GenBank/cor6_6.gb", "gb"): ... print(record.id) ... X55053.1 X62281.1 M81224.1 AJ237582.1 L31939.1 AF297471.1 Equivalently, >>> with open("GenBank/cor6_6.gb") as handle: ... for record in GenBankIterator(handle): ... print(record.id) ... X55053.1 X62281.1 M81224.1 AJ237582.1 L31939.1 AF297471.1 """ # This calls a generator function: return GenBankScanner(debug=0).parse_records(handle) def EmblIterator(handle): """Break up an EMBL file into SeqRecord objects. Every section from the LOCUS line to the terminating // becomes a single SeqRecord with associated annotation and features. Note that for genomes or chromosomes, there is typically only one record. This gets called internally by Bio.SeqIO for the EMBL file format: >>> from Bio import SeqIO >>> for record in SeqIO.parse("EMBL/epo_prt_selection.embl", "embl"): ... print(record.id) ... A00022.1 A00028.1 A00031.1 A00034.1 A00060.1 A00071.1 A00072.1 A00078.1 CQ797900.1 Equivalently, >>> with open("EMBL/epo_prt_selection.embl") as handle: ... for record in EmblIterator(handle): ... print(record.id) ... A00022.1 A00028.1 A00031.1 A00034.1 A00060.1 A00071.1 A00072.1 A00078.1 CQ797900.1 """ # This calls a generator function: return EmblScanner(debug=0).parse_records(handle) def ImgtIterator(handle): """Break up an IMGT file into SeqRecord objects. Every section from the LOCUS line to the terminating // becomes a single SeqRecord with associated annotation and features. Note that for genomes or chromosomes, there is typically only one record. """ # This calls a generator function: return _ImgtScanner(debug=0).parse_records(handle) def GenBankCdsFeatureIterator(handle, alphabet=Alphabet.generic_protein): """Break up a Genbank file into SeqRecord objects for each CDS feature. Every section from the LOCUS line to the terminating // can contain many CDS features. These are returned as with the stated amino acid translation sequence (if given). """ # This calls a generator function: return GenBankScanner(debug=0).parse_cds_features(handle, alphabet) def EmblCdsFeatureIterator(handle, alphabet=Alphabet.generic_protein): """Break up a EMBL file into SeqRecord objects for each CDS feature. Every section from the LOCUS line to the terminating // can contain many CDS features. These are returned as with the stated amino acid translation sequence (if given). """ # This calls a generator function: return EmblScanner(debug=0).parse_cds_features(handle, alphabet) def _insdc_feature_position_string(pos, offset=0): """Build a GenBank/EMBL position string (PRIVATE). Use offset=1 to add one to convert a start position from python counting. """ if isinstance(pos, SeqFeature.ExactPosition): return "%i" % (pos.position + offset) elif isinstance(pos, SeqFeature.WithinPosition): return "(%i.%i)" % (pos.position + offset, pos.position + pos.extension + offset) elif isinstance(pos, SeqFeature.BetweenPosition): return "(%i^%i)" % (pos.position + offset, pos.position + pos.extension + offset) elif isinstance(pos, SeqFeature.BeforePosition): return "<%i" % (pos.position + offset) elif isinstance(pos, SeqFeature.AfterPosition): return ">%i" % (pos.position + offset) elif isinstance(pos, SeqFeature.OneOfPosition): return "one-of(%s)" \ % ",".join(_insdc_feature_position_string(p, offset) for p in pos.position_choices) elif isinstance(pos, SeqFeature.AbstractPosition): raise NotImplementedError("Please report this as a bug in Biopython.") else: raise ValueError("Expected a SeqFeature position object.") def _insdc_location_string_ignoring_strand_and_subfeatures(location, rec_length): if location.ref: ref = "%s:" % location.ref else: ref = "" assert not location.ref_db if isinstance(location.start, SeqFeature.ExactPosition) \ and isinstance(location.end, SeqFeature.ExactPosition) \ and location.start.position == location.end.position: # Special case, for 12:12 return 12^13 # (a zero length slice, meaning the point between two letters) if location.end.position == rec_length: # Very special case, for a between position at the end of a # sequence (used on some circular genomes, Bug 3098) we have # N:N so return N^1 return "%s%i^1" % (ref, rec_length) else: return "%s%i^%i" % (ref, location.end.position, location.end.position + 1) if isinstance(location.start, SeqFeature.ExactPosition) \ and isinstance(location.end, SeqFeature.ExactPosition) \ and location.start.position + 1 == location.end.position: # Special case, for 11:12 return 12 rather than 12..12 # (a length one slice, meaning a single letter) return "%s%i" % (ref, location.end.position) elif isinstance(location.start, SeqFeature.UnknownPosition) \ or isinstance(location.end, SeqFeature.UnknownPosition): # Special case for features from SwissProt/UniProt files if isinstance(location.start, SeqFeature.UnknownPosition) \ and isinstance(location.end, SeqFeature.UnknownPosition): # warnings.warn("Feature with unknown location", BiopythonWarning) # return "?" raise ValueError("Feature with unknown location") elif isinstance(location.start, SeqFeature.UnknownPosition): # Treat the unknown start position as a BeforePosition return "%s<%i..%s" \ % (ref, location.nofuzzy_end, _insdc_feature_position_string(location.end)) else: # Treat the unknown end position as an AfterPosition return "%s%s..>%i" \ % (ref, _insdc_feature_position_string(location.start, +1), location.nofuzzy_start + 1) else: # Typical case, e.g. 12..15 gets mapped to 11:15 return ref \ + _insdc_feature_position_string(location.start, +1) \ + ".." + \ _insdc_feature_position_string(location.end) def _insdc_location_string(location, rec_length): """Build a GenBank/EMBL location from a (Compound) FeatureLocation (PRIVATE). There is a choice of how to show joins on the reverse complement strand, GenBank used "complement(join(1,10),(20,100))" while EMBL used to use "join(complement(20,100),complement(1,10))" instead (but appears to have now adopted the GenBank convention). Notice that the order of the entries is reversed! This function therefore uses the first form. In this situation we expect the CompoundFeatureLocation and its parts to all be marked as strand == -1, and to be in the order 19:100 then 0:10. """ try: parts = location.parts # CompoundFeatureLocation if location.strand == -1: # Special case, put complement outside the join/order/... and reverse order return "complement(%s(%s))" % (location.operator, ",".join(_insdc_location_string_ignoring_strand_and_subfeatures(p, rec_length) for p in parts[::-1])) else: return "%s(%s)" % (location.operator, ",".join(_insdc_location_string(p, rec_length) for p in parts)) except AttributeError: # Simple FeatureLocation loc = _insdc_location_string_ignoring_strand_and_subfeatures(location, rec_length) if location.strand == -1: return "complement(%s)" % loc else: return loc class _InsdcWriter(SequentialSequenceWriter): """Base class for GenBank and EMBL writers (PRIVATE).""" MAX_WIDTH = 80 QUALIFIER_INDENT = 21 QUALIFIER_INDENT_STR = " " * QUALIFIER_INDENT QUALIFIER_INDENT_TMP = " %s " # 21 if %s is empty FTQUAL_NO_QUOTE = ("anticodon", "citation", "codon_start", "compare", "direction", "estimated_length", "mod_base", "number", "rpt_type", "rpt_unit_range", "tag_peptide", "transl_except", "transl_table") def _write_feature_qualifier(self, key, value=None, quote=None): if value is None: # Value-less entry like /pseudo self.handle.write("%s/%s\n" % (self.QUALIFIER_INDENT_STR, key)) return if type(value) == str: value = value.replace('"', '""') # NCBI says escape " as "" in qualifier values # Quick hack with no line wrapping, may be useful for testing: # self.handle.write('%s/%s="%s"\n' % (self.QUALIFIER_INDENT_STR, key, value)) if quote is None: # Try to mimic unwritten rules about when quotes can be left out: if _is_int_or_long(value) or key in self.FTQUAL_NO_QUOTE: quote = False else: quote = True if quote: line = '%s/%s="%s"' % (self.QUALIFIER_INDENT_STR, key, value) else: line = '%s/%s=%s' % (self.QUALIFIER_INDENT_STR, key, value) if len(line) <= self.MAX_WIDTH: self.handle.write(line + "\n") return while line.lstrip(): if len(line) <= self.MAX_WIDTH: self.handle.write(line + "\n") return # Insert line break... for index in range(min(len(line) - 1, self.MAX_WIDTH), self.QUALIFIER_INDENT + 1, -1): if line[index] == " ": break if line[index] != " ": # No nice place to break... index = self.MAX_WIDTH assert index <= self.MAX_WIDTH self.handle.write(line[:index] + "\n") line = self.QUALIFIER_INDENT_STR + line[index:].lstrip() def _wrap_location(self, location): """Split a feature location into lines (break at commas) (PRIVATE).""" # TODO - Rewrite this not to recurse! length = self.MAX_WIDTH - self.QUALIFIER_INDENT if len(location) <= length: return location index = location[:length].rfind(",") if index == -1: # No good place to split (!) warnings.warn("Couldn't split location:\n%s" % location, BiopythonWarning) return location return location[:index + 1] + "\n" + \ self.QUALIFIER_INDENT_STR + \ self._wrap_location(location[index + 1:]) def _write_feature(self, feature, record_length): """Write a single SeqFeature object to features table (PRIVATE).""" assert feature.type, feature location = _insdc_location_string(feature.location, record_length) f_type = feature.type.replace(" ", "_") line = (self.QUALIFIER_INDENT_TMP % f_type)[:self.QUALIFIER_INDENT] \ + self._wrap_location(location) + "\n" self.handle.write(line) # Now the qualifiers... # Note as of Biopython 1.69, this is an ordered-dict, don't sort it: for key, values in feature.qualifiers.items(): if isinstance(values, (list, tuple)): for value in values: self._write_feature_qualifier(key, value) else: # String, int, etc - or None for a /pseudo tpy entry self._write_feature_qualifier(key, values) @staticmethod def _get_annotation_str(record, key, default=".", just_first=False): """Get an annotation dictionary entry (as a string) (PRIVATE). Some entries are lists, in which case if just_first=True the first entry is returned. If just_first=False (default) this verifies there is only one entry before returning it. """ try: answer = record.annotations[key] except KeyError: return default if isinstance(answer, list): if not just_first: assert len(answer) == 1 return str(answer[0]) else: return str(answer) @staticmethod def _split_multi_line(text, max_len): """Return a list of strings (PRIVATE). Any single words which are too long get returned as a whole line (e.g. URLs) without an exception or warning. """ # TODO - Do the line spliting while preserving white space? text = text.strip() if len(text) <= max_len: return [text] words = text.split() text = "" while words and len(text) + 1 + len(words[0]) <= max_len: text += " " + words.pop(0) text = text.strip() # assert len(text) <= max_len answer = [text] while words: text = words.pop(0) while words and len(text) + 1 + len(words[0]) <= max_len: text += " " + words.pop(0) text = text.strip() # assert len(text) <= max_len answer.append(text) assert not words return answer def _split_contig(self, record, max_len): """Return a list of strings, splits on commas (PRIVATE).""" # TODO - Merge this with _write_multi_line method? # It would need the addition of the comma splitting logic... # are there any other cases where that would be sensible? contig = record.annotations.get("contig", "") if isinstance(contig, (list, tuple)): contig = "".join(contig) contig = self.clean(contig) answer = [] while contig: if len(contig) > max_len: # Split lines at the commas pos = contig[:max_len - 1].rfind(",") if pos == -1: raise ValueError("Could not break up CONTIG") text, contig = contig[:pos + 1], contig[pos + 1:] else: text, contig = contig, "" answer.append(text) return answer class GenBankWriter(_InsdcWriter): """GenBank writer.""" HEADER_WIDTH = 12 QUALIFIER_INDENT = 21 STRUCTURED_COMMENT_START = "-START##" STRUCTURED_COMMENT_END = "-END##" STRUCTURED_COMMENT_DELIM = " :: " LETTERS_PER_LINE = 60 SEQUENCE_INDENT = 9 def _write_single_line(self, tag, text): """Write single line in each GenBank record (PRIVATE). Used in the 'header' of each GenBank record. """ assert len(tag) < self.HEADER_WIDTH if len(text) > self.MAX_WIDTH - self.HEADER_WIDTH: if tag: warnings.warn("Annotation %r too long for %r line" % (text, tag), BiopythonWarning) else: # Can't give such a precise warning warnings.warn("Annotation %r too long" % text, BiopythonWarning) self.handle.write("%s%s\n" % (tag.ljust(self.HEADER_WIDTH), text.replace("\n", " "))) def _write_multi_line(self, tag, text): """Write multiple lines in each GenBank record (PRIVATE). Used in the 'header' of each GenBank record. """ # TODO - Do the line spliting while preserving white space? max_len = self.MAX_WIDTH - self.HEADER_WIDTH lines = self._split_multi_line(text, max_len) self._write_single_line(tag, lines[0]) for line in lines[1:]: self._write_single_line("", line) def _write_multi_entries(self, tag, text_list): # used for DBLINK and any similar later line types. # If the list of strings is empty, nothing is written. for i, text in enumerate(text_list): if i == 0: self._write_single_line(tag, text) else: self._write_single_line("", text) @staticmethod def _get_date(record): default = "01-JAN-1980" try: date = record.annotations["date"] except KeyError: return default # Cope with a list of one string: if isinstance(date, list) and len(date) == 1: date = date[0] if isinstance(date, datetime): date = date.strftime("%d-%b-%Y").upper() months = ["JAN", "FEB", "MAR", "APR", "MAY", "JUN", "JUL", "AUG", "SEP", "OCT", "NOV", "DEC"] if not isinstance(date, basestring) or len(date) != 11: return default try: datetime(int(date[-4:]), months.index(date[3:6]) + 1, int(date[0:2])) except ValueError: date = default return date @staticmethod def _get_data_division(record): try: division = record.annotations["data_file_division"] except KeyError: division = "UNK" if division in ["PRI", "ROD", "MAM", "VRT", "INV", "PLN", "BCT", "VRL", "PHG", "SYN", "UNA", "EST", "PAT", "STS", "GSS", "HTG", "HTC", "ENV", "CON"]: # Good, already GenBank style # PRI - primate sequences # ROD - rodent sequences # MAM - other mammalian sequences # VRT - other vertebrate sequences # INV - invertebrate sequences # PLN - plant, fungal, and algal sequences # BCT - bacterial sequences [plus archea] # VRL - viral sequences # PHG - bacteriophage sequences # SYN - synthetic sequences # UNA - unannotated sequences # EST - EST sequences (expressed sequence tags) # PAT - patent sequences # STS - STS sequences (sequence tagged sites) # GSS - GSS sequences (genome survey sequences) # HTG - HTGS sequences (high throughput genomic sequences) # HTC - HTC sequences (high throughput cDNA sequences) # ENV - Environmental sampling sequences # CON - Constructed sequences # # (plus UNK for unknown) pass else: # See if this is in EMBL style: # Division Code # ----------------- ---- # Bacteriophage PHG - common # Environmental Sample ENV - common # Fungal FUN - map to PLN (plants + fungal) # Human HUM - map to PRI (primates) # Invertebrate INV - common # Other Mammal MAM - common # Other Vertebrate VRT - common # Mus musculus MUS - map to ROD (rodent) # Plant PLN - common # Prokaryote PRO - map to BCT (poor name) # Other Rodent ROD - common # Synthetic SYN - common # Transgenic TGN - ??? map to SYN ??? # Unclassified UNC - map to UNK # Viral VRL - common # # (plus XXX for submiting which we can map to UNK) embl_to_gbk = {"FUN": "PLN", "HUM": "PRI", "MUS": "ROD", "PRO": "BCT", "UNC": "UNK", "XXX": "UNK", } try: division = embl_to_gbk[division] except KeyError: division = "UNK" assert len(division) == 3 return division def _get_topology(self, record): """Set the topology to 'circular', 'linear' if defined (PRIVATE).""" max_topology_len = len("circular") topology = self._get_annotation_str(record, "topology", default="") if topology and len(topology) <= max_topology_len: return topology.ljust(max_topology_len) else: return " " * max_topology_len def _write_the_first_line(self, record): """Write the LOCUS line (PRIVATE).""" locus = record.name if not locus or locus == "": locus = record.id if not locus or locus == "": locus = self._get_annotation_str( record, "accession", just_first=True) if len(locus) > 16: if len(locus) + 1 + len(str(len(record))) > 28: # Locus name and record length to long to squeeze in. raise ValueError("Locus identifier %r is too long" % locus) else: warnings.warn("Stealing space from length field to allow long name in LOCUS line", BiopythonWarning) if len(locus.split()) > 1: # locus could be unicode, and u'with space' versus 'with space' # causes trouble with doctest or print-and-compare tests, so tmp = repr(locus) if tmp.startswith("u'") and tmp.endswith("'"): tmp = tmp[1:] raise ValueError("Invalid whitespace in %s for LOCUS line" % tmp) if len(record) > 99999999999: # Currently GenBank only officially support up to 350000, but # the length field can take eleven digits raise ValueError("Sequence too long!") # Get the base alphabet (underneath any Gapped or StopCodon encoding) a = Alphabet._get_base_alphabet(record.seq.alphabet) if not isinstance(a, Alphabet.Alphabet): raise TypeError("Invalid alphabet") elif isinstance(a, Alphabet.ProteinAlphabet): units = "aa" elif isinstance(a, Alphabet.NucleotideAlphabet): units = "bp" else: # Must be something like NucleotideAlphabet or # just the generic Alphabet (default for fasta files) raise ValueError("Need a Nucleotide or Protein alphabet") # Get the molecule type mol_type = self._get_annotation_str(record, "molecule_type", default=None) if mol_type and len(mol_type) > 7: # Deal with common cases from EMBL to GenBank mol_type = mol_type.replace("unassigned ", "").replace("genomic ", "") if len(mol_type) > 7: warnings.warn("Molecule type %r too long" % mol_type, BiopythonWarning) mol_type = None if mol_type in ["protein", "PROTEIN"]: mol_type = "" if mol_type: pass elif isinstance(a, Alphabet.ProteinAlphabet): mol_type = "" elif isinstance(a, Alphabet.DNAAlphabet): mol_type = "DNA" elif isinstance(a, Alphabet.RNAAlphabet): mol_type = "RNA" else: # Must be something like NucleotideAlphabet or # just the generic Alphabet (default for fasta files) raise ValueError("Need a DNA, RNA or Protein alphabet") topology = self._get_topology(record) division = self._get_data_division(record) name_length = str(len(record)).rjust(28) name_length = locus + name_length[len(locus):] assert len(name_length) == 28, name_length assert " " in name_length, name_length assert len(units) == 2 assert len(division) == 3 line = "LOCUS %s %s %s %s %s %s\n" \ % (name_length, units, mol_type.ljust(7), topology, division, self._get_date(record)) assert len(line) == 79 + 1, repr(line) # plus one for new line # We're bending the rules to allow an identifier over 16 characters # if we can steal spaces from the length field: # assert line[12:28].rstrip() == locus, \ # 'LOCUS line does not contain the locus at the expected position:\n' + line # assert line[28:29] == " " # assert line[29:40].lstrip() == str(len(record)), \ # 'LOCUS line does not contain the length at the expected position:\n' + line assert line[12:40].split() == [locus, str(len(record))], line # Tests copied from Bio.GenBank.Scanner if line[40:44] not in [' bp ', ' aa ']: raise ValueError('LOCUS line does not contain size units at ' 'expected position:\n' + line) if line[44:47] not in [' ', 'ss-', 'ds-', 'ms-']: raise ValueError('LOCUS line does not have valid strand ' 'type (Single stranded, ...):\n' + line) if not (line[47:54].strip() == "" or 'DNA' in line[47:54].strip().upper() or 'RNA' in line[47:54].strip().upper()): raise ValueError('LOCUS line does not contain valid ' 'sequence type (DNA, RNA, ...):\n' + line) if line[54:55] != ' ': raise ValueError('LOCUS line does not contain space at ' 'position 55:\n' + line) if line[55:63].strip() not in ['', 'linear', 'circular']: raise ValueError('LOCUS line does not contain valid ' 'entry (linear, circular, ...):\n' + line) if line[63:64] != ' ': raise ValueError('LOCUS line does not contain space at ' 'position 64:\n' + line) if line[67:68] != ' ': raise ValueError('LOCUS line does not contain space at ' 'position 68:\n' + line) if line[70:71] != '-': raise ValueError('LOCUS line does not contain - at ' 'position 71 in date:\n' + line) if line[74:75] != '-': raise ValueError('LOCUS line does not contain - at ' 'position 75 in date:\n' + line) self.handle.write(line) def _write_references(self, record): number = 0 for ref in record.annotations["references"]: if not isinstance(ref, SeqFeature.Reference): continue number += 1 data = str(number) # TODO - support more complex record reference locations? if ref.location and len(ref.location) == 1: a = Alphabet._get_base_alphabet(record.seq.alphabet) if isinstance(a, Alphabet.ProteinAlphabet): units = "residues" else: units = "bases" data += " (%s %i to %i)" % (units, ref.location[0].nofuzzy_start + 1, ref.location[0].nofuzzy_end) self._write_single_line("REFERENCE", data) if ref.authors: # We store the AUTHORS data as a single string self._write_multi_line(" AUTHORS", ref.authors) if ref.consrtm: # We store the consortium as a single string self._write_multi_line(" CONSRTM", ref.consrtm) if ref.title: # We store the title as a single string self._write_multi_line(" TITLE", ref.title) if ref.journal: # We store this as a single string - holds the journal name, # volume, year, and page numbers of the citation self._write_multi_line(" JOURNAL", ref.journal) if ref.medline_id: # This line type is obsolete and was removed from the GenBank # flatfile format in April 2005. Should we write it? # Note this has a two space indent: self._write_multi_line(" MEDLINE", ref.medline_id) if ref.pubmed_id: # Note this has a THREE space indent: self._write_multi_line(" PUBMED", ref.pubmed_id) if ref.comment: self._write_multi_line(" REMARK", ref.comment) def _write_comment(self, record): # This is a bit complicated due to the range of possible # ways people might have done their annotation... # Currently the parser uses a single string with newlines. # A list of lines is also reasonable. # A single (long) string is perhaps the most natural of all. # This means we may need to deal with line wrapping. lines = [] if "structured_comment" in record.annotations: comment = record.annotations["structured_comment"] # Find max length of keys for equal padded printing padding = 0 for key, data in comment.items(): for subkey, subdata in data.items(): padding = len(subkey) if len(subkey) > padding else padding # Construct output for key, data in comment.items(): lines.append("##{0}{1}".format(key, self.STRUCTURED_COMMENT_START)) for subkey, subdata in data.items(): spaces = " " * (padding - len(subkey)) lines.append("{0}{1}{2}{3}".format(subkey, spaces, self.STRUCTURED_COMMENT_DELIM, subdata)) lines.append("##{0}{1}".format(key, self.STRUCTURED_COMMENT_END)) if "comment" in record.annotations: comment = record.annotations["comment"] if isinstance(comment, basestring): lines += comment.split("\n") elif isinstance(comment, (list, tuple)): lines += list(comment) else: raise ValueError("Could not understand comment annotation") self._write_multi_line("COMMENT", lines[0]) for line in lines[1:]: self._write_multi_line("", line) def _write_contig(self, record): max_len = self.MAX_WIDTH - self.HEADER_WIDTH lines = self._split_contig(record, max_len) self._write_single_line("CONTIG", lines[0]) for text in lines[1:]: self._write_single_line("", text) def _write_sequence(self, record): # Loosely based on code from Howard Salis # TODO - Force lower case? if isinstance(record.seq, UnknownSeq): # We have already recorded the length, and there is no need # to record a long sequence of NNNNNNN...NNN or whatever. if "contig" in record.annotations: self._write_contig(record) else: self.handle.write("ORIGIN\n") return # Catches sequence being None: data = self._get_seq_string(record).lower() seq_len = len(data) self.handle.write("ORIGIN\n") for line_number in range(0, seq_len, self.LETTERS_PER_LINE): self.handle.write(str(line_number + 1).rjust(self.SEQUENCE_INDENT)) for words in range(line_number, min(line_number + self.LETTERS_PER_LINE, seq_len), 10): self.handle.write(" %s" % data[words:words + 10]) self.handle.write("\n") def write_record(self, record): """Write a single record to the output file.""" handle = self.handle self._write_the_first_line(record) default = record.id if default.count(".") == 1 and default[default.index(".") + 1:].isdigit(): # Good, looks like accesion.version and not something # else like identifier.start-end default = record.id.split(".", 1)[0] accession = self._get_annotation_str(record, "accession", default, just_first=True) acc_with_version = accession if record.id.startswith(accession + "."): try: acc_with_version = "%s.%i" \ % (accession, int(record.id.split(".", 1)[1])) except ValueError: pass gi = self._get_annotation_str(record, "gi", just_first=True) descr = record.description if descr == "": descr = "" # Trailing dot will be added later # The DEFINITION field must end with a period # see ftp://ftp.ncbi.nih.gov/genbank/gbrel.txt [3.4.5] # and discussion https://github.com/biopython/biopython/pull/616 # So let's add a period descr += '.' self._write_multi_line("DEFINITION", descr) self._write_single_line("ACCESSION", accession) if gi != ".": self._write_single_line("VERSION", "%s GI:%s" % (acc_with_version, gi)) else: self._write_single_line("VERSION", "%s" % acc_with_version) # The NCBI initially expected two types of link, # e.g. "Project:28471" and "Trace Assembly Archive:123456" # # This changed and at some point the formatting switched to # include a space after the colon, e.g. # # LOCUS NC_000011 1606 bp DNA linear CON 06-JUN-2016 # DEFINITION Homo sapiens chromosome 11, GRCh38.p7 Primary Assembly. # ACCESSION NC_000011 REGION: complement(5225466..5227071) GPC_000001303 # VERSION NC_000011.10 GI:568815587 # DBLINK BioProject: PRJNA168 # Assembly: GCF_000001405.33 # ... # # Or, # # LOCUS JU120277 1044 bp mRNA linear TSA 27-NOV-2012 # DEFINITION TSA: Tupaia chinensis tbc000002.Tuchadli mRNA sequence. # ACCESSION JU120277 # VERSION JU120277.1 GI:379775257 # DBLINK BioProject: PRJNA87013 # Sequence Read Archive: SRR433859 # ... dbxrefs_with_space = [] for x in record.dbxrefs: if ": " not in x: x = x.replace(":", ": ") dbxrefs_with_space.append(x) self._write_multi_entries("DBLINK", dbxrefs_with_space) del dbxrefs_with_space try: # List of strings # Keywords should be given separated with semi colons, keywords = "; ".join(record.annotations["keywords"]) # with a trailing period: if not keywords.endswith("."): keywords += "." except KeyError: # If no keywords, there should be just a period: keywords = "." self._write_multi_line("KEYWORDS", keywords) if "segment" in record.annotations: # Deal with SEGMENT line found only in segmented records, # e.g. AH000819 segment = record.annotations["segment"] if isinstance(segment, list): assert len(segment) == 1, segment segment = segment[0] self._write_single_line("SEGMENT", segment) self._write_multi_line("SOURCE", self._get_annotation_str(record, "source")) # The ORGANISM line MUST be a single line, as any continuation is the taxonomy org = self._get_annotation_str(record, "organism") if len(org) > self.MAX_WIDTH - self.HEADER_WIDTH: org = org[:self.MAX_WIDTH - self.HEADER_WIDTH - 4] + "..." self._write_single_line(" ORGANISM", org) try: # List of strings # Taxonomy should be given separated with semi colons, taxonomy = "; ".join(record.annotations["taxonomy"]) # with a trailing period: if not taxonomy.endswith("."): taxonomy += "." except KeyError: taxonomy = "." self._write_multi_line("", taxonomy) if "references" in record.annotations: self._write_references(record) if "comment" in record.annotations or "structured_comment" in record.annotations: self._write_comment(record) handle.write("FEATURES Location/Qualifiers\n") rec_length = len(record) for feature in record.features: self._write_feature(feature, rec_length) self._write_sequence(record) handle.write("//\n") class EmblWriter(_InsdcWriter): """EMBL writer.""" HEADER_WIDTH = 5 QUALIFIER_INDENT = 21 QUALIFIER_INDENT_STR = "FT" + " " * (QUALIFIER_INDENT - 2) QUALIFIER_INDENT_TMP = "FT %s " # 21 if %s is empty # Note second spacer line of just FH is expected: FEATURE_HEADER = "FH Key Location/Qualifiers\nFH\n" LETTERS_PER_BLOCK = 10 BLOCKS_PER_LINE = 6 LETTERS_PER_LINE = LETTERS_PER_BLOCK * BLOCKS_PER_LINE POSITION_PADDING = 10 def _write_contig(self, record): max_len = self.MAX_WIDTH - self.HEADER_WIDTH lines = self._split_contig(record, max_len) for text in lines: self._write_single_line("CO", text) def _write_sequence(self, record): handle = self.handle # save looking up this multiple times if isinstance(record.seq, UnknownSeq): # We have already recorded the length, and there is no need # to record a long sequence of NNNNNNN...NNN or whatever. if "contig" in record.annotations: self._write_contig(record) else: # TODO - Can the sequence just be left out as in GenBank files? handle.write("SQ \n") return # Catches sequence being None data = self._get_seq_string(record).lower() seq_len = len(data) # Get the base alphabet (underneath any Gapped or StopCodon encoding) a = Alphabet._get_base_alphabet(record.seq.alphabet) if isinstance(a, Alphabet.DNAAlphabet): # TODO - What if we have RNA? a_count = data.count('A') + data.count('a') c_count = data.count('C') + data.count('c') g_count = data.count('G') + data.count('g') t_count = data.count('T') + data.count('t') other = seq_len - (a_count + c_count + g_count + t_count) handle.write("SQ Sequence %i BP; %i A; %i C; %i G; %i T; %i other;\n" % (seq_len, a_count, c_count, g_count, t_count, other)) else: handle.write("SQ \n") for line_number in range(0, seq_len // self.LETTERS_PER_LINE): handle.write(" ") # Just four, not five for block in range(self.BLOCKS_PER_LINE): index = self.LETTERS_PER_LINE * line_number + \ self.LETTERS_PER_BLOCK * block handle.write((" %s" % data[index:index + self.LETTERS_PER_BLOCK])) handle.write(str((line_number + 1) * self.LETTERS_PER_LINE).rjust(self.POSITION_PADDING)) handle.write("\n") if seq_len % self.LETTERS_PER_LINE: # Final (partial) line line_number = (seq_len // self.LETTERS_PER_LINE) handle.write(" ") # Just four, not five for block in range(self.BLOCKS_PER_LINE): index = self.LETTERS_PER_LINE * line_number + \ self.LETTERS_PER_BLOCK * block handle.write( (" %s" % data[index:index + self.LETTERS_PER_BLOCK]).ljust(11)) handle.write(str(seq_len).rjust(self.POSITION_PADDING)) handle.write("\n") def _write_single_line(self, tag, text): assert len(tag) == 2 line = tag + " " + text if len(text) > self.MAX_WIDTH: warnings.warn("Line %r too long" % line, BiopythonWarning) self.handle.write(line + "\n") def _write_multi_line(self, tag, text): max_len = self.MAX_WIDTH - self.HEADER_WIDTH lines = self._split_multi_line(text, max_len) for line in lines: self._write_single_line(tag, line) def _write_the_first_lines(self, record): """Write the ID and AC lines (PRIVATE).""" if "." in record.id and record.id.rsplit(".", 1)[1].isdigit(): version = "SV " + record.id.rsplit(".", 1)[1] accession = self._get_annotation_str(record, "accession", record.id.rsplit(".", 1)[0], just_first=True) else: version = "" accession = self._get_annotation_str(record, "accession", record.id, just_first=True) if ";" in accession: raise ValueError("Cannot have semi-colon in EMBL accession, %s" % repr(str(accession))) if " " in accession: # This is out of practicallity... might it be allowed? raise ValueError("Cannot have spaces in EMBL accession, %s" % repr(str(accession))) topology = self._get_annotation_str(record, "topology", default="") # Get the molecule type # TODO - record this explicitly in the parser? # Get the base alphabet (underneath any Gapped or StopCodon encoding) a = Alphabet._get_base_alphabet(record.seq.alphabet) if not isinstance(a, Alphabet.Alphabet): raise TypeError("Invalid alphabet") elif isinstance(a, Alphabet.DNAAlphabet): mol_type = "DNA" units = "BP" elif isinstance(a, Alphabet.RNAAlphabet): mol_type = "RNA" units = "BP" elif isinstance(a, Alphabet.ProteinAlphabet): mol_type = "PROTEIN" units = "AA" else: # Must be something like NucleotideAlphabet raise ValueError("Need a DNA, RNA or Protein alphabet") if record.annotations.get("molecule_type", None): # Note often get RNA vs DNA discrepancy in real EMBL/NCBI files mol_type = record.annotations["molecule_type"] if mol_type in ["protein"]: mol_type = "PROTEIN" # Get the taxonomy division division = self._get_data_division(record) # TODO - Full ID line handle = self.handle # ID <1>; SV <2>; <3>; <4>; <5>; <6>; <7> BP. # 1. Primary accession number # 2. Sequence version number # 3. Topology: 'circular' or 'linear' # 4. Molecule type # 5. Data class # 6. Taxonomic division # 7. Sequence length self._write_single_line("ID", "%s; %s; %s; %s; ; %s; %i %s." % (accession, version, topology, mol_type, division, len(record), units)) handle.write("XX\n") self._write_single_line("AC", accession + ";") handle.write("XX\n") @staticmethod def _get_data_division(record): try: division = record.annotations["data_file_division"] except KeyError: division = "UNC" if division in ["PHG", "ENV", "FUN", "HUM", "INV", "MAM", "VRT", "MUS", "PLN", "PRO", "ROD", "SYN", "TGN", "UNC", "VRL", "XXX"]: # Good, already EMBL style # Division Code # ----------------- ---- # Bacteriophage PHG # Environmental Sample ENV # Fungal FUN # Human HUM # Invertebrate INV # Other Mammal MAM # Other Vertebrate VRT # Mus musculus MUS # Plant PLN # Prokaryote PRO # Other Rodent ROD # Synthetic SYN # Transgenic TGN # Unclassified UNC (i.e. unknown) # Viral VRL # # (plus XXX used for submiting data to EMBL) pass else: # See if this is in GenBank style & can be converted. # Generally a problem as the GenBank groups are wider # than those of EMBL. Note that GenBank use "BCT" for # both bacteria and acherea thus this maps to EMBL's # "PRO" nicely. gbk_to_embl = {"BCT": "PRO", "UNK": "UNC", } try: division = gbk_to_embl[division] except KeyError: division = "UNC" assert len(division) == 3 return division def _write_keywords(self, record): # Put the keywords right after DE line. # Each 'keyword' can have multiple words and spaces, but we # must not split any 'keyword' between lines. # TODO - Combine short keywords onto one line for keyword in record.annotations["keywords"]: self._write_single_line("KW", keyword) self.handle.write("XX\n") def _write_references(self, record): # The order should be RN, RC, RP, RX, RG, RA, RT, RL number = 0 for ref in record.annotations["references"]: if not isinstance(ref, SeqFeature.Reference): continue number += 1 self._write_single_line("RN", "[%i]" % number) # TODO - support for RC line (needed in parser too) # TODO - support more complex record reference locations? if ref.location and len(ref.location) == 1: self._write_single_line( "RP", "%i-%i" % (ref.location[0].nofuzzy_start + 1, ref.location[0].nofuzzy_end)) # TODO - record any DOI or AGRICOLA identifier in the reference object? if ref.pubmed_id: self._write_single_line("RX", "PUBMED; %s." % ref.pubmed_id) if ref.consrtm: self._write_single_line("RG", "%s" % ref.consrtm) if ref.authors: # We store the AUTHORS data as a single string self._write_multi_line("RA", ref.authors + ";") if ref.title: # We store the title as a single string self._write_multi_line("RT", '"%s";' % ref.title) if ref.journal: # We store this as a single string - holds the journal name, # volume, year, and page numbers of the citation self._write_multi_line("RL", ref.journal) self.handle.write("XX\n") def _write_comment(self, record): # This is a bit complicated due to the range of possible # ways people might have done their annotation... # Currently the parser uses a single string with newlines. # A list of lines is also reasonable. # A single (long) string is perhaps the most natural of all. # This means we may need to deal with line wrapping. comment = record.annotations["comment"] if isinstance(comment, basestring): lines = comment.split("\n") elif isinstance(comment, (list, tuple)): lines = comment else: raise ValueError("Could not understand comment annotation") # TODO - Merge this with the GenBank comment code? if not lines: return for line in lines: self._write_multi_line("CC", line) self.handle.write("XX\n") def write_record(self, record): """Write a single record to the output file.""" handle = self.handle self._write_the_first_lines(record) # PR line (0 or 1 lines only), project identifier # # Assuming can't use 2 lines, we should prefer newer GenBank # DBLINK BioProject:... entries over the older GenBank DBLINK # Project:... lines. # # In either case, seems EMBL usess just "PR Project:..." # regardless of the type of ID (old numeric only, or new # with alpha prefix), e.g. for CP002497 NCBI now uses: # # DBLINK BioProject: PRJNA60715 # BioSample: SAMN03081426 # # While EMBL uses: # # XX # PR Project:PRJNA60715; # XX # # Sorting ensures (new) BioProject:... is before old Project:... for xref in sorted(record.dbxrefs): if xref.startswith("BioProject:"): self._write_single_line("PR", xref[3:] + ";") handle.write("XX\n") break if xref.startswith("Project:"): self._write_single_line("PR", xref + ";") handle.write("XX\n") break # TODO - DT lines (date) descr = record.description if descr == "": descr = "." self._write_multi_line("DE", descr) handle.write("XX\n") if "keywords" in record.annotations: self._write_keywords(record) # Should this be "source" or "organism"? self._write_multi_line( "OS", self._get_annotation_str(record, "organism")) try: # List of strings taxonomy = "; ".join(record.annotations["taxonomy"]) + "." except KeyError: taxonomy = "." self._write_multi_line("OC", taxonomy) handle.write("XX\n") if "references" in record.annotations: self._write_references(record) if "comment" in record.annotations: self._write_comment(record) handle.write(self.FEATURE_HEADER) rec_length = len(record) for feature in record.features: self._write_feature(feature, rec_length) handle.write("XX\n") self._write_sequence(record) handle.write("//\n") class ImgtWriter(EmblWriter): """IMGT writer (EMBL format variant).""" HEADER_WIDTH = 5 QUALIFIER_INDENT = 25 # Not 21 as in EMBL QUALIFIER_INDENT_STR = "FT" + " " * (QUALIFIER_INDENT - 2) QUALIFIER_INDENT_TMP = "FT %s " # 25 if %s is empty FEATURE_HEADER = "FH Key Location/Qualifiers\nFH\n" if __name__ == "__main__": from Bio._utils import run_doctest run_doctest(verbose=0)