LOCUS NZ_ACVX01000034 38231 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00017, whole genome shotgun sequence. ACCESSION NZ_ACVX01000034 NZ_ACVX00000000 VERSION NZ_ACVX01000034.1 GI:256005582 DBLINK Project: 55637 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. REFERENCE 1 (bases 1 to 38231) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Hemme,C.L. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The draft genome of Clostridium thermocellum DSM 2360 JOURNAL Unpublished REFERENCE 2 (bases 1 to 38231) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Hemme,C.L. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (17-JUL-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000034. URL -- http://www.jgi.doe.gov JGI Project ID: 4085028 Source DNA and Bacteria available from Christopher L. Hemme (hemmecl@ou.edu) Contacts: Christopher L. Hemme (hemmecl@ou.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Annotation by JGI-ORNL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Clostridium thermocellum DSM 2360 Culture Collection ID :: DSM 2360 GOLD Stamp ID :: Gi02594 Isolation Site :: contaminant of Clostridium thermocellum strain LQ8 Isolation Country :: USA Oxygen Requirement :: Anaerobe Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Sporulating Temperature Range :: Thermophile Temperature Optimum :: 60 C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Phenotypes :: Cellulose degrader, Ethanogenic, Ethanol production Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Location/Qualifiers source 1..38231 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene <1..647 /locus_tag="ClothDRAFT_2403" CDS <1..647 /locus_tag="ClothDRAFT_2403" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: swd:Swoo_3891 peptidase S41" /codon_start=3 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430542.1" /db_xref="GI:256005583" /translation="KSKILCYRFLENSEVRRFEICEYDIKSKKFGEPIFIPGDSQKFE GSVKYVPNSDRISFVLDYELHIYDRSSGTLTKLFEVAFNSYSWDNTGKKLLYSDYDEN IYIYDMESNEKRKILTGICPVYSSSNEYIAYIGIDQRLTVYNVNTGKKWRTVPVDDSA RYIFSPDDKYILLGTEYADLMSFPHYTLYILDYKTGKKKRLFGGEGNVPSLDWK" misc_feature 138..>641 /locus_tag="ClothDRAFT_2403" /note="WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues...; Region: WD40; cl02567" /db_xref="CDD:154987" misc_feature order(171..173,183..185,201..206,240..242,288..290, 300..302,318..323,357..359,396..398,411..413,429..434, 468..470,516..518,537..539,573..578,612..614) /locus_tag="ClothDRAFT_2403" /note="structural tetrad; other site" /db_xref="CDD:29257" gene 1523..2056 /locus_tag="ClothDRAFT_2404" CDS 1523..2056 /locus_tag="ClothDRAFT_2404" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430543.1" /db_xref="GI:256005584" /translation="MDFDFAQFSTIKKKTLGGMKIVSFLKKHVPALVLILILLIGATA LFFYINEKEKQDYIEIFHRNKSIFEEISTELMPLEYGTTVSKEAGKIVVKQNGNITEI FQYPIDEETKTNIRRAINDFGVKSINKTDECLKFIYSSKRDFHALVYTADKSKLTGFT DVEHLEGNWYYCYILYE" gene 2249..2551 /locus_tag="ClothDRAFT_2405" CDS 2249..2551 /locus_tag="ClothDRAFT_2405" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430544.1" /db_xref="GI:256005585" /translation="MKYKREKTFHFRLTKYAKSVLGSELYSNKIFIHKIDKENKVYNQ DVVQLMKRLYGIDMEKSQYPKLRCEIPPVDIVITMGCNMKYSGEISGGILLKLESG" gene 2617..3462 /locus_tag="ClothDRAFT_2406" CDS 2617..3462 /locus_tag="ClothDRAFT_2406" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: gur:Gura_1472 YD repeat-containing protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430545.1" /db_xref="GI:256005586" /translation="MQIKITPEEMTRIAGNIREVSDKFNDIALEVKRIINSIDWELRS RKGINEKSSIAQATAIKISDRLNKMSQSLIRARDKMIEADNKASATAKKMKTVSFKRT VAAATLSNYPSDVEYVDPLWNHVVGPGTANCPNTYVGDPINVASGNFYLTRRDIAIPS RGMGLEITRYYNSMDNTQGIFGKGWRIDYETCLKKKEDSEDIIVVYPEGNIRVFEYTD TGDFKSPKGVYDTLFKAEDGTYILKVQKGITYKYDQAGSLISISDSNNKRYNSNTIAR DCCLR" misc_feature 2620..2868 /locus_tag="ClothDRAFT_2406" /note="Proteins of 100 residues with WXG; Region: WXG100; cl02005" /db_xref="CDD:154701" gene 3486..8138 /locus_tag="ClothDRAFT_2407" CDS 3486..8138 /locus_tag="ClothDRAFT_2407" /inference="protein motif:TFAM:TIGR01643" /note="TIGRFAM: YD repeat protein; PFAM: YD repeat-containing protein; KEGG: hch:HCH_02073 rhs family protein" /codon_start=1 /transl_table=11 /product="YD repeat protein" /protein_id="ZP_05430546.1" /db_xref="GI:256005587" /db_xref="InterPro:IPR006530" /translation="MFSYEGYRVSSITDHTGRKLKYKYDEKGNLTQVIYPDGGKITYA YDDVGLISVTDQNGNTYVQNTYDKNGRVIKQLDHQENELIIEYDDENRENTFKWLKSG ITRVYKYNTDMLVTEVRYDDGSVQKYTYDENLNRNSETDRNGNTTCKKYDDKGNLIEV TSPEPFCYKTKYSYDENGRLIKTVSPGGGEVTFEYDDRGNLLKRTLKTGSRSYSEWSY TYDQYGRITSSRDAENNITSFEYGEEDVNKPTLIKDAVGNTFRYEFDKVGRVTAITTG YGTVRLRYDDCDRITHITDAEGNIIRLNYDKAGNMTKIIAPKQYAEKGENGAGYTFEY DAMDKLIKTIDPLGNVFAVKYDENGNKIKEINPNYYNSQKDDGIGIEYKYDTNHRRIS TIFPDGSMSRTKYDPQGNIIKTISWKDYDKDLDDGPGMEYTYDEMNRLTQIIDPEGNV IKKYIYDEEGRIVKEIDAKGYSSADNDEERWGTIYKYNLAGWLVEKRTPLRQKDGEIY YNIIEYVHDKNGRVVKEKRSPEYVTKSEYPKKWNVINYKYDPNGNLIEVTDSLGASIT YEYDSLGKKTLEKTKINDKKQKVTRYVYNSVGRLVKVIQELDGEDLSGYSNEKVLAET IYNYDPNGNLIEVISPEGYLTVFKYDDANRRIKSILYQPQNGVKLIGSAYCALLNTKA RSISYEYDRAGNLVKQILPNGGVIINEYDEMNRKIKVTDPDGNTTRIFYDNTGNIVKY VEPGNYNPEKDDGVGTTYLYDSMNRLIQIVNAAGIVVEKNIYNTAGEIIKKIDSVGYK SADNDNDRHGVEFSYDLAGRLVEITTPEAKIHGRKSQQYTYDAEGNITGIVDGNGNST RYSLDLWGKIINITEPDGTNIKYDYDYAGNLVSTTDGNRNTTRYTYNSLNLLSEIIDP DGRKITFKYDRQGRMVQRIGKDGHSTYYTYNADNNITGCWEEEGQMERYEYNIDGSLA ASISGTTIHTYTYTLAGRLKSKTTNGRKVLEYDYNKNGFVSRLVDISGTPVEYTYDVL GRVAAITNGGKISAKYEYNIDNTIAQVLYGSGICARYEYDLDKNITRLLNTDPTGKEM FVYKYAYDGNGNPILKEENGKVTAYGYDALNRLQEVIYPGNVKERFTYDANGNRLKRE FRDILEQYEYDNCNRLIQRIKNGLVTEYEYDEKGNLIKEKEGELTKLYSYDGFDRLIR VQNPDGTYMENIYDAEDLRTISIENGRYNRYIYSGRNIACEVDEDWRLKDRIVRGHTI LQKEDGNKNAYYYIHNSHGDITALTDDKGKIANAYSYDAFGNILDSVERVDNRFKYSG EVYDSVAGQYYLRARYYNPSIGRFIQEDPFRGDGLNLYAYVANNPVRYIDPTGNCKEG VNFNNNLEETTKVIVEKNESKGNSVWDIVKDIFVDTIEGAVDEIIAKKVIERLPNYSA KTLMGHAKFGYAFEATKVKPIPGVKGLKSFSKVAGPVAVMVYGYEVYQDIKKYDGWDA VKAITVTTVATAVTFLAGFILAQAALPVVATLIVSAAVGIAIGFFADWVKKKWIGY" misc_feature 3786..6020 /locus_tag="ClothDRAFT_2407" /note="Rhs family protein [Cell envelope biogenesis, outer membrane]; Region: RhsA; COG3209" /db_xref="CDD:33022" misc_feature 4005..4118 /locus_tag="ClothDRAFT_2407" /note="RHS Repeat; Region: RHS_repeat; cl11982" /db_xref="CDD:159659" misc_feature 5127..>5213 /locus_tag="ClothDRAFT_2407" /note="RHS Repeat; Region: RHS_repeat; cl11982" /db_xref="CDD:159659" misc_feature 5520..7604 /locus_tag="ClothDRAFT_2407" /note="Rhs family protein [Cell envelope biogenesis, outer membrane]; Region: RhsA; COG3209" /db_xref="CDD:33022" misc_feature 5550..5663 /locus_tag="ClothDRAFT_2407" /note="RHS Repeat; Region: RHS_repeat; cl11982" /db_xref="CDD:159659" misc_feature 5922..6059 /locus_tag="ClothDRAFT_2407" /note="RHS Repeat; Region: RHS_repeat; cl11982" /db_xref="CDD:159659" misc_feature 6126..6239 /locus_tag="ClothDRAFT_2407" /note="RHS Repeat; Region: RHS_repeat; cl11982" /db_xref="CDD:159659" misc_feature 7380..7604 /locus_tag="ClothDRAFT_2407" /note="RHS repeat-associated core domain; Region: Rhs_assc_core; TIGR03696" /db_xref="CDD:163408" gene 8170..8547 /locus_tag="ClothDRAFT_2408" CDS 8170..8547 /locus_tag="ClothDRAFT_2408" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430547.1" /db_xref="GI:256005588" /translation="MSLNYLPLPVYVSLCLFLSIALILIAVKRRNVDRKGLLVLYLIG SAGGILATIIRILKERTSIYDDYFNQIGYILFGYIILLFADIFYISMTQKVDKESRKK ILIGWVMIAVSILAGVAVYFILD" gene 8918..9160 /locus_tag="ClothDRAFT_2409" CDS 8918..9160 /locus_tag="ClothDRAFT_2409" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430548.1" /db_xref="GI:256005589" /translation="MIEADNKASTTAKGMKTASFKTTVVAATLSNYLSVVEYIDPLRN HIVGSGTANCANTYVGDPVNAVSGNFYLTRRDIAIP" gene 9363..9554 /locus_tag="ClothDRAFT_2410" CDS 9363..9554 /locus_tag="ClothDRAFT_2410" /inference="protein motif:TFAM:TIGR01643" /note="TIGRFAM: YD repeat protein" /codon_start=1 /transl_table=11 /product="YD repeat protein" /protein_id="ZP_05430549.1" /db_xref="GI:256005590" /db_xref="InterPro:IPR006530" /translation="MLFKTKDGTYILKVKKGITYKYDQAGSLISISDPNNNEIQLKYN REGLLSTVMLTGGKLLMFS" gene 9648..9977 /locus_tag="ClothDRAFT_2411" CDS 9648..9977 /locus_tag="ClothDRAFT_2411" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430550.1" /db_xref="GI:256005591" /translation="MSLNFLPLQVYVSLCLFLSIALILIAVKRRNIDRKGLLVFYFIS SAGGILAAIIRVLKETTSIYDDYFNQIGYILFGYAGLFFADLFYLSVTQVIAKGDKEA KRKLIIG" gene 10594..12717 /locus_tag="ClothDRAFT_2412" CDS 10594..12717 /locus_tag="ClothDRAFT_2412" /inference="protein motif:PFAM:PF05270" /note="PFAM: alpha-L-arabinofuranosidase B; glycoside hydrolase family 43; cellulosome protein dockerin type I; KEGG: sde:Sde_2809 ATPase" /codon_start=1 /transl_table=11 /product="alpha-L-arabinofuranosidase B" /protein_id="ZP_05430551.1" /db_xref="GI:256005592" /db_xref="InterPro:IPR002048" /db_xref="InterPro:IPR002105" /db_xref="InterPro:IPR006710" /db_xref="InterPro:IPR007934" /translation="MANRTFKFIVLLFTTVLFATSVCYSTNPITKAKFQSYNYPNMYI RHANFDARIDENVTPEMDSQWELVPGLANSGDGYVSIQSVNYPGYYLRHSNYDLSLEK NDGTSLFAESATFKIVPGLADPSYISFQSYNFPTRYIRHYNYLLRLDEIVTELDRQDA TFKIISEDTQPPAPTLIGDVNADGKIDSTDLTLLKRYLLRSATLTEEKILNADTDGNG TVNSTDLNYLKKYILRVISVFPAEGNKPPTPTPTKTPVATPSPTQPLFTPSFKDVTVH DPSVIKTNGTYYVIGSHLAFAKTNDLIQWQQINSSVYTGNPLIPNVFDELKEAFEWAK TDTMWAGDIIQLRDGKYYMYYCMCKGDSPLSALGVARADKVEGPYKNLGILLRSGAGK GEDGTNYNATIHPNAVDPHTFFDKNGDLWMVYGSYSGGIYILKMNPSTGKQYPGQGYG KKLLGANHSRIEGPYIQYSPDTGYYYLFLSFGGLTADGGYNIRVARSQNPDGPYYDAE GNNMINCRGPAGSFFDDRAIEPYGVKLMGNFRFVDSGIGYVSPGHNSTYYDEESGKYF IIFHTRFPGKGEMHQVRVHQMFINEDGWPVIAPHRYAGETIRRYSPEEVAGTYAYINH GRDISANIKNSVRISLNKDGTVSGNGVNGRWELSGEHYIRLTVNGTNYTGVVLQQWDD GLKKYVMTFSAISRNGNVTIWGSQL" sig_peptide 10594..10671 /locus_tag="ClothDRAFT_2412" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.987) with cleavage site probability 0.883 at residue 26" misc_feature 10690..11079 /locus_tag="ClothDRAFT_2412" /note="Alpha-L-arabinofuranosidase B (ABFB); Region: AbfB; pfam05270" /db_xref="CDD:114019" misc_feature 11128..11190 /locus_tag="ClothDRAFT_2412" /note="Dockerin type I repeat; Region: Dockerin_1; pfam00404" /db_xref="CDD:144118" misc_feature 11416..12384 /locus_tag="ClothDRAFT_2412" /note="Glycosyl hydrolase family 43; Region: GH43_ABN_1; cd08998" /db_xref="CDD:185739" misc_feature order(11416..11424,11467..11472,11608..11610,11809..11811, 11869..11874,11977..11979,12043..12045,12061..12063, 12331..12333) /locus_tag="ClothDRAFT_2412" /note="substrate binding site; other site" /db_xref="CDD:185739" misc_feature order(11422..11424,11818..11820,11977..11979) /locus_tag="ClothDRAFT_2412" /note="active site" /db_xref="CDD:185739" gene 13081..13560 /locus_tag="ClothDRAFT_2413" CDS 13081..13560 /locus_tag="ClothDRAFT_2413" /inference="protein motif:PFAM:PF00210" /note="PFAM: Ferritin Dps family protein; KEGG: ftm:FTM_1206 ferric iron binding protein, ferritin-like protein" /codon_start=1 /transl_table=11 /product="Ferritin Dps family protein" /protein_id="ZP_05430552.1" /db_xref="GI:256005593" /db_xref="InterPro:IPR008331" /db_xref="InterPro:IPR009040" /translation="MSKLYDNLNEQMNFELESAYIYATMSAYLDSLNMKGMTHFFDEQ VKEEMEHAGKIKKFLQDTGYGIKYRPLNPGDGKFDSIEDVFKKALAHEKTVTSRINDL VKQAREEGDQRVLNLLAWFVDEQIEEENMFGVLVERLERINGHWNGLYILDKELGER" misc_feature 13084..13548 /locus_tag="ClothDRAFT_2413" /note="nonheme-containing ferritins; Region: Nonheme_Ferritin; cd01055" /db_xref="CDD:153113" misc_feature 13096..13506 /locus_tag="ClothDRAFT_2413" /note="Ferritin-like domain; Region: Ferritin; pfam00210" /db_xref="CDD:143967" misc_feature order(13123..13125,13219..13224,13231..13233,13354..13356, 13450..13452,13459..13464) /locus_tag="ClothDRAFT_2413" /note="ferroxidase diiron center; other site" /db_xref="CDD:153113" gene 14117..15979 /locus_tag="ClothDRAFT_2414" CDS 14117..15979 /locus_tag="ClothDRAFT_2414" /inference="protein motif:PFAM:PF07556" /note="PFAM: protein of unknown function DUF1538; KEGG: aeh:Mlg_0659 hypothetical protein" /codon_start=1 /transl_table=11 /product="protein of unknown function DUF1538" /protein_id="ZP_05430553.1" /db_xref="GI:256005594" /db_xref="InterPro:IPR011435" /translation="MKKLNLKSKLAIFATTLKEVFISSLPLAAIMIIVCGFIAPLDSG AEYVKLFVGYASVVFGQALFLDGLNISILPIGKLVGGSLIKLKKSIFVIFFGLLFGVL ATVAEPALTVLAKQTNMIMPIINETVFIWIMGFGIGVMVAFSLFRIMKDLNIKVVFAI LYVITFLLIIFVPDEFVALAFDGSGATTGDISVPFILALGMGVSTTMSRHKSNDDSFG IIGLASVGPIISLAIYGIVLKFLYNGVFPPEQVYSPETVGTVGEIIVNNLWGVTLALL PVIIVFLPFQFLLIKQPKKEFVKILLGTVVVFIGLLIFLAGIDYGFAFAGKYIGEVFL DPSRPEWFKWLLLIVAFILGAAITLSEPAVTVLGEQLEEMTNGHIAKMTIRMTLAIGI GFAALLGMLKILTEINILWFLIPLYAVALIMMIFAPKLFVGLAFDSGGVSGGALTSAF LTPLTLGVAQAVAATSPSGGQPILVNGFGIIAFISVTPLIAVQFLGIVYNINIKKAEK ALKDAEMNDIKELASLAGIVEEVAAEKSATQESIAEKAAVQESIVEKGGIEKSVVDEE SITEKSIVKENIDEDDIAAKMDEQRQNEQHHEDNIVKDMKDGHEELKAGKNSAE" sig_peptide 14117..14251 /locus_tag="ClothDRAFT_2414" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.957) with cleavage site probability 0.590 at residue 45" misc_feature 14195..14830 /locus_tag="ClothDRAFT_2414" /note="Protein of unknown function (DUF1538); Region: DUF1538; pfam07556" /db_xref="CDD:148909" misc_feature 14948..15613 /locus_tag="ClothDRAFT_2414" /note="Protein of unknown function (DUF1538); Region: DUF1538; pfam07556" /db_xref="CDD:148909" misc_feature <15623..15967 /locus_tag="ClothDRAFT_2414" /note="MAEBL; Provisional; Region: PTZ00121" /db_xref="CDD:173412" gene 16007..16321 /locus_tag="ClothDRAFT_2415" CDS 16007..16321 /locus_tag="ClothDRAFT_2415" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430554.1" /db_xref="GI:256005595" /translation="MTEYKHGVDFLTLIADRKYKKEILKFLFDFRCHLIDVIYVKGTV KSEYFRDMLGLVNEEKKVMITGLIAGNLSEQLLEELVTKLNFDKPNTGIAFVVPVNKL SV" gene 16337..16663 /locus_tag="ClothDRAFT_2416" CDS 16337..16663 /locus_tag="ClothDRAFT_2416" /inference="similar to AA sequence:KEGG:DP0349" /note="KEGG: dps:DP0349 nitrogen regulatory protein P-II family proteins" /codon_start=1 /transl_table=11 /product="nitrogen regulatory protein P-II family protein" /protein_id="ZP_05430555.1" /db_xref="GI:256005596" /translation="MESTNMKALYIVVNAGFAEQVVDYIRSQGSTGATIINARGVSAL HKEIMGISVDTEKEVVLTVTESEIADKIMEAIKLNTAFRTEAQGICFTLPVSKAVGLR KRSSSE" gene 16858..17820 /locus_tag="ClothDRAFT_2417" CDS 16858..17820 /locus_tag="ClothDRAFT_2417" /EC_number="2.8.1.6" /inference="protein motif:TFAM:TIGR00433" /note="KEGG: dps:DP2549 biotin synthetase BioB; TIGRFAM: biotin synthase; PFAM: biotin and thiamin synthesis associated; Radical SAM domain protein; SMART: Elongator protein 3/MiaB/NifB" /codon_start=1 /transl_table=11 /product="biotin synthase" /protein_id="ZP_05430556.1" /db_xref="GI:256005597" /db_xref="InterPro:IPR002684" /db_xref="InterPro:IPR006638" /db_xref="InterPro:IPR007197" /db_xref="InterPro:IPR010722" /translation="MDFLTVMENKLDEGRMITFEEAVELAKGKIEDEKLFLLADKLCK KNMGRRVDLCSIINAKSGGCSENCKFCAQSGHYDTGVKIYPLLDVDDVLKAAKENEKE GVHRFSLVTSGKSVSDEEFEKILGIYSVLRKETNLKLCASLGSLSYERAVRLKEAGVS MYHHNIETCREYYPKICDTHTYDDRINTVKNAAKAGLEICCGGIIGMGESMEQRIKMA FEIRELGVKSVPINVLNPIKGTPFENVRSLSPDEILRTIALFRLIMPYAHIRYAGGRM CLGEHQSKGFKAGVSAMLVGNYLTTVGNKISDDLEMIQRMGLEI" misc_feature 16858..17817 /locus_tag="ClothDRAFT_2417" /note="biotin synthase; Validated; Region: PRK06256" /db_xref="CDD:180492" misc_feature 17038..17628 /locus_tag="ClothDRAFT_2417" /note="Radical SAM superfamily. Enzymes of this family generate radicals by combining a 4Fe-4S cluster and S-adenosylmethionine (SAM) in close proximity. They are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster...; Region: Radical_SAM; cd01335" /db_xref="CDD:100105" misc_feature order(17047..17049,17053..17055,17059..17061,17065..17073, 17188..17190,17194..17199,17275..17283,17350..17352, 17467..17469,17554..17559) /locus_tag="ClothDRAFT_2417" /note="FeS/SAM binding site; other site" /db_xref="CDD:100105" misc_feature 17545..17817 /locus_tag="ClothDRAFT_2417" /note="Biotin and Thiamin Synthesis associated domain; Region: BATS; cl06149" /db_xref="CDD:157061" gene 17817..18551 /locus_tag="ClothDRAFT_2418" CDS 17817..18551 /locus_tag="ClothDRAFT_2418" /EC_number="6.3.3.3" /inference="protein motif:TFAM:TIGR00347" /note="TIGRFAM: dethiobiotin synthase; KEGG: gsu:GSU1583 dethiobiotin synthase" /codon_start=1 /transl_table=11 /product="dethiobiotin synthase" /protein_id="ZP_05430557.1" /db_xref="GI:256005598" /db_xref="InterPro:IPR004472" /translation="MNGTKGIYIIGTDTDVGKTVVCAGLMYLLKSKGYDCCYFKPVSS GGRQTENGFFSYDVSFVKEVSGFNEDDEMINPFRFKTPVSPHLASEAEGFAVDKEIIL DRYRKLSEKYRYIVAEGCGGLAVPLSRDGYMQFQLIKEMGLSCILVSRTVLGTINHTL LTLSFAQNAGIPIKGIIFSGFSDRTLERDNIETIRKLSKVPVLGVVPKIDGISVEIIN AEDKILGELKSVFEKTINIEELLANV" misc_feature 17835..18443 /locus_tag="ClothDRAFT_2418" /note="CobQ/CobB/MinD/ParA nucleotide binding domain; Region: CbiA; pfam01656" /db_xref="CDD:145019" misc_feature 17835..>17948 /locus_tag="ClothDRAFT_2418" /note="Ras-like GTPase superfamily. The Ras-like superfamily of small GTPases consists of several families with an extremely high degree of structural and functional similarity. The Ras superfamily is divided into at least four families in eukaryotes: the Ras...; Region: Ras_like_GTPase; cl10444" /db_xref="CDD:186996" misc_feature <18129..18437 /locus_tag="ClothDRAFT_2418" /note="Ras-like GTPase superfamily. The Ras-like superfamily of small GTPases consists of several families with an extremely high degree of structural and functional similarity. The Ras superfamily is divided into at least four families in eukaryotes: the Ras...; Region: Ras_like_GTPase; cl10444" /db_xref="CDD:186996" gene 18544..19710 /locus_tag="ClothDRAFT_2419" CDS 18544..19710 /locus_tag="ClothDRAFT_2419" /EC_number="2.3.1.47" /inference="protein motif:TFAM:TIGR00858" /note="KEGG: gsu:GSU2629 8-amino-7-oxononanoate synthase; TIGRFAM: 8-amino-7-oxononanoate synthase; PFAM: aminotransferase class I and II; aminotransferase class-III" /codon_start=1 /transl_table=11 /product="8-amino-7-oxononanoate synthase" /protein_id="ZP_05430558.1" /db_xref="GI:256005599" /db_xref="InterPro:IPR001917" /db_xref="InterPro:IPR004723" /db_xref="InterPro:IPR004839" /db_xref="InterPro:IPR005814" /translation="MYDFQGALKDIKNKGLYREFRNVNAAQGPYTVIDGRKMLMMSSN NYLGLCDDIRLKRAAIESIRKFGVGAGGSRLTCGNFELHRELEERLAKFKDVESCIVF GSGYAANIGAISGIADKNWVIFCDRLNHASIVDGIRLSGAKLVVYKHCDMEDLESKIV RYHTGKSLIVTDGVFSMDGDVAPVDRIVKLAKKYNLMTMVDDAHATGILGEKGRGTSE YFGLKDAVDISMGTLSKAFGVEGGFVAGKRKLVDFLRHKAKSFIYSTAPPPYNMAAAL EALNIIETEPQARKELAEKSVWLRNRLIEKGFNVPKGVTPIIPLMVGDVNTAVEFSML LYNEGIYIPAIRPPTVPKGTSRLRISIMASHSYEDMEFALKNLVRFGRKLGIIP" misc_feature 18544..19686 /locus_tag="ClothDRAFT_2419" /note="7-keto-8-aminopelargonate synthetase and related enzymes [Coenzyme metabolism]; Region: BioF; COG0156" /db_xref="CDD:30505" misc_feature 18649..19686 /locus_tag="ClothDRAFT_2419" /note="KBL_like; this family belongs to the pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The major groups in this CD corresponds to serine palmitoyltransferase (SPT), 5-aminolevulinate synthase (ALAS), 8-amino-7-...; Region: KBL_like; cd06454" /db_xref="CDD:99747" misc_feature order(18673..18675,18853..18861,18931..18933,19057..19059, 19069..19074,19144..19146,19150..19155,19237..19239, 19246..19248,19615..19617) /locus_tag="ClothDRAFT_2419" /note="substrate-cofactor binding pocket; other site" /db_xref="CDD:99747" misc_feature order(18853..18861,18931..18933,19057..19059,19069..19071, 19144..19146,19150..19155,19237..19239,19246..19248) /locus_tag="ClothDRAFT_2419" /note="pyridoxal 5'-phosphate binding site; other site" /db_xref="CDD:99747" misc_feature 19246..19248 /locus_tag="ClothDRAFT_2419" /note="catalytic residue; other site" /db_xref="CDD:99747" gene 19710..20486 /locus_tag="ClothDRAFT_2420" CDS 19710..20486 /locus_tag="ClothDRAFT_2420" /EC_number="3.1.1.1" /inference="protein motif:PRIAM:3.1.1.1" /note="KEGG: gbm:Gbem_0832 alpha/beta hydrolase fold" /codon_start=1 /transl_table=11 /product="Carboxylesterase" /protein_id="ZP_05430559.1" /db_xref="GI:256005600" /db_xref="InterPro:IPR003089" /translation="MLTGEDNGLEKQKLVMMPGWSMDSSVWKPVVAALSEYFQLIFCE WHGITGMEGYTERVCKLIEKEKDKLCLLGWSLGSLLAIDAAGKYKDRVEKLVLVGGTA RFTRDKETGYFCGWHKSAVKKMKEAIKVHREKTMDSFYSSLFSSKEQESKEYVALFKV DIDTNTASFKELEAGLDFLLAADMRMQLESIKADTLIIHGEMDSICPKEAALYMAQRM KNKAQVFIMEGIGHVPFYSLPEEFTRLILEFMKQGDKSDR" misc_feature 19743..20456 /locus_tag="ClothDRAFT_2420" /note="putative pimeloyl-BioC--CoA transferase BioH; Region: bioH; TIGR01738" /db_xref="CDD:130799" misc_feature <19959..20453 /locus_tag="ClothDRAFT_2420" /note="Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These...; Region: Esterase_lipase; cl12031" /db_xref="CDD:187168" gene 20476..21327 /locus_tag="ClothDRAFT_2421" CDS 20476..21327 /locus_tag="ClothDRAFT_2421" /inference="protein motif:TFAM:TIGR02072" /note="TIGRFAM: biotin biosynthesis protein BioC; PFAM: Methyltransferase type 11; NodS family protein; Methyltransferase type 12; KEGG: gsu:GSU2627 biotin synthesis protein, putative" /codon_start=1 /transl_table=11 /product="biotin biosynthesis protein BioC" /protein_id="ZP_05430560.1" /db_xref="GI:256005601" /db_xref="InterPro:IPR008715" /db_xref="InterPro:IPR011814" /db_xref="InterPro:IPR013216" /db_xref="InterPro:IPR013217" /translation="MIDKKILQMHFSRNAKNYDAYAKVQKKMANTLLDMLDLDSKNRL DILDVGCGTGYLTKLLLDRWPDARITAIDIAPGMIEYARDRFNESNVEFACLDIEEAE LNQKYDLVISNATFQWFNDLGGTVNKLVQSLKSDGVLAFSTFGHMTFSELHFSYETAR RKLKIDEEFPPGQKFCNAKEILKICCETFEGLEGFEFDTVKKESLEYEYFYTVREFLD SVKKIGANNSNKQRKVNTALTKEMIRIYEEMFKVNGLVRATYHCIFITSRKKLAANTR RLVNAVV" misc_feature 20503..21282 /locus_tag="ClothDRAFT_2421" /note="biotin biosynthesis protein BioC; Region: BioC; TIGR02072" /db_xref="CDD:162684" misc_feature 20608..20907 /locus_tag="ClothDRAFT_2421" /note="S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy)...; Region: AdoMet_MTases; cd02440" /db_xref="CDD:100107" misc_feature order(20620..20640,20692..20697,20761..20769,20812..20814) /locus_tag="ClothDRAFT_2421" /note="S-adenosylmethionine binding site; other site" /db_xref="CDD:100107" gene 21374..22693 /locus_tag="ClothDRAFT_2422" CDS 21374..22693 /locus_tag="ClothDRAFT_2422" /inference="protein motif:PFAM:PF00202" /note="PFAM: aminotransferase class-III; KEGG: pca:Pcar_1460 adenosylmethionine-8-amino-7-oxononanoate aminotransferase" /codon_start=1 /transl_table=11 /product="aminotransferase class-III" /protein_id="ZP_05430561.1" /db_xref="GI:256005602" /db_xref="InterPro:IPR005814" /translation="MKDYEDFPPIVIERGEGPYIFDVLGNRYIDAVSSWWVNLFGHCN KRINDAVKRQLDSLEHVIFANFSHRPAIELAGKIVEITPQGLNKVFFADNGSSAVEIA LKLSFQYHLQTGSCEKKKFVALSDAYHGETLGALAVGGLDLYSKIYKPLMMSTIKVPG PDCYRCKYGKERTCCEAECFCEMEKILEEKHREICGVIIEPMVQAAAGMKIYSAKYLI KLREACSYYNIHLIADEIAVGFGRTGKMFACEHGGISPDIMCLSKGLTAGYMPLSLVV TTQKIYDAFYSDYIELKAFLHSHSYTGNPLACAAALESLKIFEDDDVLNKNREKSGLF RKLAESLAHSHPYVGEFRQIGMIAALELVENKAQKKEFDWKKRVGYNIYKLALKKGLL LRPLGNVLYFMPPYVVGQKDIEDIVSGAFYAINEYFGLDFKYKRMFP" misc_feature 21374..22648 /locus_tag="ClothDRAFT_2422" /note="adenosylmethionine--8-amino-7-oxononanoate transaminase; Provisional; Region: PRK07030" /db_xref="CDD:180800" misc_feature 21383..22627 /locus_tag="ClothDRAFT_2422" /note="Acetyl ornithine aminotransferase family. This family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The major groups in this CD correspond to ornithine aminotransferase, acetylornithine aminotransferase...; Region: OAT_like; cd00610" /db_xref="CDD:99735" misc_feature order(21653..21661,21755..21760,21764..21766,21971..21973, 22073..22075,22079..22084,22160..22162) /locus_tag="ClothDRAFT_2422" /note="inhibitor-cofactor binding pocket; inhibition site" /db_xref="CDD:99735" misc_feature order(21656..21661,21755..21760,21971..21973,22073..22075, 22082..22084,22160..22162) /locus_tag="ClothDRAFT_2422" /note="pyridoxal 5'-phosphate binding site; other site" /db_xref="CDD:99735" misc_feature 22160..22162 /locus_tag="ClothDRAFT_2422" /note="catalytic residue; other site" /db_xref="CDD:99735" gene 23013..23453 /locus_tag="ClothDRAFT_2423" CDS 23013..23453 /locus_tag="ClothDRAFT_2423" /inference="similar to AA sequence:KEGG:VP1875" /note="KEGG: vpa:VP1875 para-aminobenzoate synthase component I" /codon_start=1 /transl_table=11 /product="para-aminobenzoate synthase component I" /protein_id="ZP_05430562.1" /db_xref="GI:256005603" /translation="MVDIKNTYVSELPFFSGAVEYFSYDLCHRIETFKEHGKEDMNIP DMIFGFYNNAIIIDHKCNKVYAAVSSIGFERREDINQVLERKINEIIKKVCEGSVKST TGKKAAEGQSYVASNFTFEEYCSIIGKVKEYIKNGDIYQANLSQ" misc_feature <23040..23207 /locus_tag="ClothDRAFT_2423" /note="Anthranilate synthase component I, N terminal region; Region: Anth_synt_I_N; pfam04715" /db_xref="CDD:147064" misc_feature <23358..>23450 /locus_tag="ClothDRAFT_2423" /note="chorismate binding enzyme; Region: Chorismate_bind; cl10555" /db_xref="CDD:187007" gene 23717..24013 /locus_tag="ClothDRAFT_2424" CDS 23717..24013 /locus_tag="ClothDRAFT_2424" /EC_number="4.1.3.27" /inference="protein motif:PRIAM:4.1.3.27" /note="PFAM: Chorismate binding-like; KEGG: pca:Pcar_0730 anthranilate synthase, component I" /codon_start=1 /transl_table=11 /product="Anthranilate synthase" /protein_id="ZP_05430563.1" /db_xref="GI:256005604" /db_xref="InterPro:IPR005801" /db_xref="InterPro:IPR015890" /translation="MIVDFERNDLGRVCKIGSVHVEKLLQIEEYSTVLHLVSTVKGEL LDDVDIIDCIKATFPEGSITGAPKIRSMETIDELKPVKRNIYTGSIGYMDFNGN" misc_feature <23717..24010 /locus_tag="ClothDRAFT_2424" /note="chorismate binding enzyme; Region: Chorismate_bind; cl10555" /db_xref="CDD:187007" gene 24023..24169 /locus_tag="ClothDRAFT_2425" CDS 24023..24169 /locus_tag="ClothDRAFT_2425" /inference="similar to AA sequence:KEGG:Dalk_1229" /note="KEGG: dal:Dalk_1229 chorismate binding-like protein" /codon_start=1 /transl_table=11 /product="chorismate binding-like protein" /protein_id="ZP_05430564.1" /db_xref="GI:256005605" /translation="MAIRTIVIKGNEAFFNVGGGIVWDSVPEDEYRETLDKGKALLKV LTGR" misc_feature <24026..24145 /locus_tag="ClothDRAFT_2425" /note="chorismate binding enzyme; Region: Chorismate_bind; cl10555" /db_xref="CDD:187007" gene 24178..24312 /locus_tag="ClothDRAFT_2426" CDS 24178..24312 /locus_tag="ClothDRAFT_2426" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430565.1" /db_xref="GI:256005606" /translation="MDCFHGIKSEDFGVLYGFSLFETFLVNEYGRVFLLEKHIDRLFN " misc_feature 24208..>24303 /locus_tag="ClothDRAFT_2426" /note="PyridoxaL 5'-Phosphate Dependent Enzymes class IV (PLPDE_IV). This D-amino acid superfamily, one of five classes of PLPDE, consists of branched-chain amino acid aminotransferases (BCAT), D-amino acid transferases (DAAT), and 4-amino-4-deoxychorismate...; Region: PLPDE_IV; cl00224" /db_xref="CDD:185842" gene 24257..24652 /locus_tag="ClothDRAFT_2427" CDS 24257..24652 /locus_tag="ClothDRAFT_2427" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430566.1" /db_xref="GI:256005607" /translation="MNTEGYSCLKSILTGCLINSMAHFGFETGFTKDNLMSMVMDYIK KYDLKNVILRLTVTYGNKNKGIEPAVFFSTRENTYKKAIYEKGFKLMVSGLVKNADSP VIAHKTGNYLENYMEGQRTLKNGFDDVIF" misc_feature 24305..>24649 /locus_tag="ClothDRAFT_2427" /note="PyridoxaL 5'-Phosphate Dependent Enzymes class IV (PLPDE_IV). This D-amino acid superfamily, one of five classes of PLPDE, consists of branched-chain amino acid aminotransferases (BCAT), D-amino acid transferases (DAAT), and 4-amino-4-deoxychorismate...; Region: PLPDE_IV; cl00224" /db_xref="CDD:185842" misc_feature order(24323..24325,24416..24418,24422..24424,24467..24469, 24581..24583,24593..24595,24599..24601,24611..24613) /locus_tag="ClothDRAFT_2427" /note="homodimer interface; other site" /db_xref="CDD:29567" misc_feature 24575..24577 /locus_tag="ClothDRAFT_2427" /note="catalytic residue; other site" /db_xref="CDD:29567" gene complement(24836..25846) /locus_tag="ClothDRAFT_2428" CDS complement(24836..25846) /locus_tag="ClothDRAFT_2428" /inference="protein motif:COG:COG3541" /note="KEGG: pmr:PMI1543 hypothetical protein" /codon_start=1 /transl_table=11 /product="nucleotidyltransferase-like protein" /protein_id="ZP_05430567.1" /db_xref="GI:256005608" /translation="MGIQDIKDRLNSDKYEFLRTDPRLGENILILTTAGSIAYGTNVD TSDIDIRGVTIETKQDLLGLSSFEQFEDRITDTVIYGLKKFISLCLDSNPNVLEILGT KDEHLLVITKEGQLLRDNADLFLSKKAIHSFGNYATAQLRRLKNALARDSYPQAEKEQ HILESILGQMEHLKRTYKSFTNEEINLYIDQSDKPEMETEIFIDINLKHYPLRDLKNI YSDMSNVVKNYSKLNHRNNKKDEPHLNKHAMHLIRLLITGTEVLEGKGINTYREKERE FLLDIRKGKYSYNELFEMVDEYERRFRLAAENTSLPDKPDYKRVEELMLEIYSNIWGF GR" misc_feature complement(25001..25792) /locus_tag="ClothDRAFT_2428" /note="Predicted nucleotidyltransferase; Region: Nuc-transf; cl01417" /db_xref="CDD:186431" misc_feature complement(24869..>25111) /locus_tag="ClothDRAFT_2428" /note="Predicted nucleotidyltransferase; Region: Nuc-transf; cl01417" /db_xref="CDD:186431" gene 26283..26927 /locus_tag="ClothDRAFT_2429" CDS 26283..26927 /locus_tag="ClothDRAFT_2429" /inference="similar to AA sequence:KEGG:HH1060" /note="KEGG: hhe:HH1060 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430568.1" /db_xref="GI:256005609" /translation="MAFDFKKEYKELYMPKTEPEIIDVPQMNFIAVRGKGDPNEEDGA YKQAVNILYAIAYTIKMSNKSGRKIDGFFEYVVPPLEGFWWQEGVEGVDYSQKDKFNW ISVIRLPDFVSKEDFEWAKEEAARKKKIDCSLAEFLTIKEGLCVQALHLGPFDDEPKT VAKMDEYLIKEGYEKDFSNERLHHEIYLSDPRKVEPAKYKTVIRHPVKKKEKYL" misc_feature 26283..26906 /locus_tag="ClothDRAFT_2429" /note="Uncharacterized conserved protein [Function unknown]; Region: COG4832" /db_xref="CDD:34441" misc_feature 26286..26537 /locus_tag="ClothDRAFT_2429" /note="Bacterial transcription activator, effector binding domain; Region: AraC_E_bind; cl01368" /db_xref="CDD:186426" gene complement(26994..28766) /locus_tag="ClothDRAFT_2430" CDS complement(26994..28766) /locus_tag="ClothDRAFT_2430" /inference="protein motif:PFAM:PF02156" /note="PFAM: glycoside hydrolase family 26; cellulosome protein dockerin type I; KEGG: hypothetical protein" /codon_start=1 /transl_table=11 /product="glycoside hydrolase family 26" /protein_id="ZP_05430569.1" /db_xref="GI:256005610" /db_xref="InterPro:IPR000805" /db_xref="InterPro:IPR002048" /db_xref="InterPro:IPR002105" /db_xref="InterPro:IPR005084" /translation="MGKIFGRTLSLLVTFAMVFSVLLVMPVSTYAAYSLPVDVEAEDC TLGNGAVVTTNVYGTQYPGYSGDGFVWVANSGTITLEVTIPENGMYELSTRCWMYLGK EDETRMQVISINGKSHSNYFIPNKGQWIDYSFGFFYLEAGKATIEIGSSGSWGFILYD KIYFDHADMPDHIIDPTPCDPNATPETRALMKYLTSVYGKYVISGQQEIYGNGNDGNY ELEFDYIYEKTGKYPAIRGFDFMNYNPLYGWEDGTTARIIDWVKNRGGIATACWHINI PRDFASYKLGEPVDWTNCTYKPTSSFNTANCLDETTKEHAYLMMAIEDLAEQLLILQE QNIPILFRPFHEAEGYNNTDGSGAWFWWGSAGAEVYKELWKLLYKTLTEKYGIHNLIW EVNLYTYANSYEWYPGDEYVDIIGYDKYEGSPNTWGTSAASSLFLTLVNYTNDTKMVA LTENDVIPDIQNIVNEEAWWLYFCPWYGDFLMSPRYNDPVLLNTIYNSEYVITLDELP ENLYEYDGEIPDINYGDLNNDGNINSTDYMILKKYILKVLERMNVPEKAADLNGDGSI NSTDLTILKRFIMKAITKFPVTQK" sig_peptide complement(28671..28766) /locus_tag="ClothDRAFT_2430" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.999) with cleavage site probability 0.910 at residue 32" misc_feature complement(27249..28229) /locus_tag="ClothDRAFT_2430" /note="Glycosyl hydrolase family 26; Region: Glyco_hydro_26; cl09200" /db_xref="CDD:158452" misc_feature complement(27132..27194) /locus_tag="ClothDRAFT_2430" /note="Dockerin type I repeat; Region: Dockerin_1; pfam00404" /db_xref="CDD:144118" misc_feature complement(27030..27092) /locus_tag="ClothDRAFT_2430" /note="Dockerin type I repeat; Region: Dockerin_1; pfam00404" /db_xref="CDD:144118" gene 29135..31801 /locus_tag="ClothDRAFT_2431" CDS 29135..31801 /locus_tag="ClothDRAFT_2431" /inference="protein motif:TFAM:TIGR00231" /note="TIGRFAM: small GTP-binding protein; PFAM: protein of unknown function DUF901; elongation factor G domain protein; protein synthesis factor GTP-binding; elongation factor G domain IV; KEGG: cjj:CJJ81176_pTet0048 TetO" /codon_start=1 /transl_table=11 /product="small GTP-binding protein" /protein_id="ZP_05430570.1" /db_xref="GI:256005611" /db_xref="InterPro:IPR000640" /db_xref="InterPro:IPR000795" /db_xref="InterPro:IPR001951" /db_xref="InterPro:IPR002127" /db_xref="InterPro:IPR005225" /db_xref="InterPro:IPR005517" /db_xref="InterPro:IPR010298" /translation="MKKLVVGILAHVDAGKTTLSEALLYVSGKIRKLGRVDNKDAYLD TYELERARGITIFSKQAVFEVGDTRITLLDTPGHVDFSAEMERTLQVLDYAILVVSGA DGVQGHTKTLWHLLEIYKIPVFIFVNKMDQNGTNKDKVINEIKKQLDDRCIEFSEENP EEFFDRLAMCDEMIMETYLEKGRVETSQISAAVKERKVFPCFFGSALKLEGVEEFIHG LMKYTVVPSYPNEFGAKIFKITRDEQGNRLTHMKLTGGKLKVRDVLTNGVWEEKVNQI RIYSGEKFEAVSEVDAGTVFAVTGLTQSRPGEGLGIEKSSGAPLLEPVLQYQIILPEG CDPRAMLPKLRQIEDEEPELNIVWDEQLQEIRVRVMGEVQIEILQSLIKSRFGVDVTF DDGSIVYKETIANTVEGVGHFEPLRHYAEVHLLLEPGERGSGLKFDVNCSEDVLAKSW QRLVLTHLEEKVHKGVLTGSAITDMKITLVSGRAHNKHTQGGDFREATYRAVRQGLKE AESILLEPYYDFQLEVPEKMVGRAMMDIEKMHGTCEISQINGDMAVLVGSAPVVTMRN YQKEVVAYTKGLGRLFCSFKGYEPCHNAQEVIERIGYDSERDVENPTGSVFCANGVSF LVSWDEVKNYMHVESYFQKKEDEENLNQNRPVYSGEKAISLEEIDQIMNKTFYANQGR KSAWKRQKASEERYYKPAAGAKRHEAKEEYLLVDGYNIIYAWPELKKLADENLDGARM KLLDMLSNYQWIRRCHVIVVFDAYRVEGHKEEIIDYYNIHVVYTREAQTADQYIEKFA YENSKNYDITVATSDGLQQIIVRGEGCALLSARELKAELEAANERIKQEYHKMNKIDR NYLGDALSTKAKRHMEDLIEEENKNNGIKKDK" misc_feature 29147..29812 /locus_tag="ClothDRAFT_2431" /note="Tet(M)-like subfamily. Tet(M), Tet(O), Tet(W), and OtrA are tetracycline resistance genes found in Gram-positive and Gram-negative bacteria. Tetracyclines inhibit protein synthesis by preventing aminoacyl-tRNA from binding to the ribosomal acceptor...; Region: TetM_like; cd04168" /db_xref="CDD:133368" misc_feature 29150..30940 /locus_tag="ClothDRAFT_2431" /note="Translation elongation factors (GTPases) [Translation, ribosomal structure and biogenesis]; Region: FusA; COG0480" /db_xref="CDD:30828" misc_feature 29162..29185 /locus_tag="ClothDRAFT_2431" /note="G1 box; other site" /db_xref="CDD:133368" misc_feature order(29165..29167,29171..29173,29183..29188,29195..29197, 29204..29209,29309..29314,29366..29371,29438..29443, 29549..29551,29561..29563) /locus_tag="ClothDRAFT_2431" /note="putative GEF interaction site; other site" /db_xref="CDD:133368" misc_feature order(29171..29188,29516..29521,29525..29527,29747..29755) /locus_tag="ClothDRAFT_2431" /note="GTP/Mg2+ binding site; other site" /db_xref="CDD:133368" misc_feature 29276..29311 /locus_tag="ClothDRAFT_2431" /note="Switch I region; other site" /db_xref="CDD:133368" misc_feature 29297..29299 /locus_tag="ClothDRAFT_2431" /note="G2 box; other site" /db_xref="CDD:133368" misc_feature 29354..29365 /locus_tag="ClothDRAFT_2431" /note="G3 box; other site" /db_xref="CDD:133368" misc_feature 29360..29416 /locus_tag="ClothDRAFT_2431" /note="Switch II region; other site" /db_xref="CDD:133368" misc_feature 29516..29527 /locus_tag="ClothDRAFT_2431" /note="G4 box; other site" /db_xref="CDD:133368" misc_feature 29747..29755 /locus_tag="ClothDRAFT_2431" /note="G5 box; other site" /db_xref="CDD:133368" misc_feature 29819..30070 /locus_tag="ClothDRAFT_2431" /note="Tet_II: This subfamily represents domain II of ribosomal protection proteins Tet(M) and Tet(O). This domain has homology to domain II of the elongation factors EF-G and EF-2. Tet(M) and Tet(O) catalyze the release of tetracycline (Tc) from the ribosome...; Region: Tet_II; cd03690" /db_xref="CDD:58081" misc_feature 30323..30664 /locus_tag="ClothDRAFT_2431" /note="EF-G_domain IV_RPP domain is a part of bacterial ribosomal protected proteins (RPP) family. RPPs such as tetracycline resistance proteins Tet(M) and Tet(O) mediate tetracycline resistance in both gram-positive and -negative species. Tetracyclines...; Region: Tet_like_IV; cd01684" /db_xref="CDD:58278" misc_feature 30677..30910 /locus_tag="ClothDRAFT_2431" /note="Tet_C: C-terminus of ribosomal protection proteins Tet(M) and Tet(O). This domain has homology to the C terminal domains of the elongation factors EF-G and EF-2. Tet(M) and Tet(O) catalyze the release of tetracycline (Tc) from the ribosome in a GTP-...; Region: Tet_C; cd03711" /db_xref="CDD:58064" misc_feature 31271..31756 /locus_tag="ClothDRAFT_2431" /note="Protein of unknown function (DUF901); Region: DUF901; cl01491" /db_xref="CDD:154430" gene complement(31918..32577) /locus_tag="ClothDRAFT_2432" CDS complement(31918..32577) /locus_tag="ClothDRAFT_2432" /inference="protein motif:PFAM:PF00027" /note="PFAM: cyclic nucleotide-binding; SMART: cyclic nucleotide-binding; KEGG: dds:Ddes_1827 transcriptional regulator, Crp/Fnr family" /codon_start=1 /transl_table=11 /product="transcriptional regulator, Crp/Fnr family" /protein_id="ZP_05430571.1" /db_xref="GI:256005612" /db_xref="InterPro:IPR000595" /db_xref="InterPro:IPR012318" /translation="MKKYLDVLKKVNLFKGIDESDLLSLLACLSSKLEHYKKNEIVFM SGEAINSFGIVLSGEVQIVQEDYYGNRSILARIGVGNLFAESFACAQIEALPVSVITT TESDLLFIDCRKLAKPCATACAFHSRLIQNMLHITAMKNISLTQKIEFISKRTTREKL LAYLSSEAQKAGSNRFSIPFNRQELADYLSVDRSAMSAELSKLRKEGILNYNKNHFEL L" misc_feature complement(32245..32478) /locus_tag="ClothDRAFT_2432" /note="effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO...; Region: CAP_ED; cd00038" /db_xref="CDD:28920" misc_feature complement(order(32287..32292,32296..32298,32323..32328)) /locus_tag="ClothDRAFT_2432" /note="ligand binding site; other site" /db_xref="CDD:28920" misc_feature complement(31921..32064) /locus_tag="ClothDRAFT_2432" /note="Winged helix-turn-helix (WHTH) DNA-binding domain of the GntR family of transcriptional regulators; Region: WHTH_GntR; cl00088" /db_xref="CDD:185792" gene 32692..33396 /locus_tag="ClothDRAFT_2433" CDS 32692..33396 /locus_tag="ClothDRAFT_2433" /inference="protein motif:PFAM:PF00037" /note="PFAM: 4Fe-4S ferredoxin iron-sulfur binding domain protein; KEGG: gsu:GSU3247 iron-sulfur cluster-binding protein" /codon_start=1 /transl_table=11 /product="4Fe-4S ferredoxin iron-sulfur binding domain protein" /protein_id="ZP_05430572.1" /db_xref="GI:256005613" /db_xref="InterPro:IPR001450" /translation="MIRRVIKIDEDKCIGCELCVNACHEGAIGMVNGKAKLLRDDYCD GLGDCLPVCPTGAITFEEREAAEYNEAAVQENKLKKQKEDLPCGCPGTQMRTINRENE HKAEPAETYENMSRLSQWPVQIKLVPVNAPYFENANLLIAADCTAYAYGNFHNKFMKN RITLIGCPKLDDVDYTEKLTAIIKNNNIKSVTVVRMEVPCCGGIENAVKRALLNSGKL IPWQVVTISTNGEILE" misc_feature 32707..>32868 /locus_tag="ClothDRAFT_2433" /note="hydrogenases, Fe-only; Region: Fe_only_hydrog; TIGR02512" /db_xref="CDD:162896" gene 33456..35084 /locus_tag="ClothDRAFT_2434" CDS 33456..35084 /locus_tag="ClothDRAFT_2434" /inference="protein motif:TFAM:TIGR01703" /note="TIGRFAM: hybrid cluster protein; PFAM: Prismane; KEGG: dds:Ddes_1829 hybrid cluster protein" /codon_start=1 /transl_table=11 /product="hybrid cluster protein" /protein_id="ZP_05430573.1" /db_xref="GI:256005614" /db_xref="InterPro:IPR004137" /db_xref="InterPro:IPR010048" /translation="MSMFCYQCQETAGGKGCTVRGVCGKNEEVAKLQDLLLYTVKGIS YIVTKGNIDAAKLGNTNHEVLSSLFMTITNVNFDDGSIEKQIRKMLAVRDEMKKSVQA EGLHDAAVFSVDSRESMLKKADSVGVLSTQNEDIRSLREMITYGVKGMAAYAEHAKNI GKEDKEIYSFIYEALAATLDDSLSVDDLFALTLKTGEYGVKVMALLDEANTSRFGNPE ITEVNIGVRKNPAILVSGHDLTDLEQLLEQTKGTGVDVYTHGEMLPAHYYPAFKKYDN FVGNYGNAWWKQVEEFESFHGPILFTTNCIVPPRSEEVRRRIFTTGSAGFPGCKHIEA DENGKKDFSEIIELAKTLPAPDEIETGSIVGGFAHNQVMALADKVVEAVKSGAVKKFF VMAGCDGRMKSRSYYTEFAQNLPKDTVILTAGCAKYRYNKLGLGDIGGIPRVLDAGQC NDSYSLAVIALKLKEVFGLDDINKLPIAFNIAWYEQKAVIVLLALLYLGVKNIHLGPT LPGFLSPNVAKVLVEKFGIAGIGTVEDDIKLFMS" misc_feature 33462..35078 /locus_tag="ClothDRAFT_2434" /note="hybrid cluster protein; Provisional; Region: PRK05290" /db_xref="CDD:179995" misc_feature 33462..>33647 /locus_tag="ClothDRAFT_2434" /note="The HCP family of iron-sulfur proteins includes hybrid cluster protein (HCP), acetyl-CoA synthase (ACS), and carbon monoxide dehydrogenase (CODH), all of which contain [Fe4-S4] metal clusters at their active sites. These proteins have a conserved alpha-; Region: HCP_like; cl14655" /db_xref="CDD:187409" misc_feature order(33567..33569,33573..33578,33585..33590) /locus_tag="ClothDRAFT_2434" /note="ACS interaction site; other site" /db_xref="CDD:73219" misc_feature order(33576..33578,33585..33587,33597..33599,33618..33620) /locus_tag="ClothDRAFT_2434" /note="CODH interaction site; other site" /db_xref="CDD:73219" misc_feature 33888..35078 /locus_tag="ClothDRAFT_2434" /note="Hybrid cluster protein (HCP), formerly known as prismane, is thought to play a role in nitrogen metabolism but its specific function is unknown. HCP has three structural domains, an N-terminal alpha-helical domain, and two similar domains comprising a...; Region: HCP; cd01914" /db_xref="CDD:73287" misc_feature order(34164..34166,34236..34238,34368..34370,34644..34646, 34728..34730,34803..34805,34908..34910) /locus_tag="ClothDRAFT_2434" /note="hybrid metal cluster; other site" /db_xref="CDD:73287" gene complement(35186..35614) /locus_tag="ClothDRAFT_2435" CDS complement(35186..35614) /locus_tag="ClothDRAFT_2435" /inference="protein motif:PFAM:PF00011" /note="PFAM: heat shock protein Hsp20; CS; KEGG: dps:DP1866 low molecular weight heat shock protein (HSP17)" /codon_start=1 /transl_table=11 /product="heat shock protein Hsp20" /protein_id="ZP_05430574.1" /db_xref="GI:256005615" /db_xref="InterPro:IPR002068" /db_xref="InterPro:IPR007052" /db_xref="InterPro:IPR017447" /translation="MSLISWNPFREVDSISRDMETFFERSPFGFFSRATAPRVDVFET EKDVVVKAEIPGVSKEDLNVYVDENSIRLSGETKRDTEYKNEHIYRTERYYGSFSRTI PLPVEVKSEQAKAEYKDGILTVTVPKVETTQMKGKKIDIH" misc_feature complement(35189..35593) /locus_tag="ClothDRAFT_2435" /note="Molecular chaperone (small heat shock protein) [Posttranslational modification, protein turnover, chaperones]; Region: IbpA; COG0071" /db_xref="CDD:30420" misc_feature complement(35231..35497) /locus_tag="ClothDRAFT_2435" /note="Alpha-crystallin domain (ACD) of alpha-crystallin-type small(s) heat shock proteins (Hsps). sHsps are small stress induced proteins with monomeric masses between 12 -43 kDa, whose common feature is the Alpha-crystallin domain (ACD). sHsps are...; Region: ACD_sHsps-like; cd06464" /db_xref="CDD:107221" misc_feature complement(order(35252..35257,35315..35317,35447..35452, 35456..35458,35462..35464,35483..35497)) /locus_tag="ClothDRAFT_2435" /note="putative dimer interface; other site" /db_xref="CDD:107221" gene 36468..36932 /locus_tag="ClothDRAFT_2436" CDS 36468..36932 /locus_tag="ClothDRAFT_2436" /inference="protein motif:PFAM:PF01584" /note="PFAM: CheW domain protein; SMART: CheW domain protein; KEGG: pca:Pcar_0995 chemotaxis signal transduction protein, purine binding protein, CheW-like" /codon_start=1 /transl_table=11 /product="CheW protein" /protein_id="ZP_05430575.1" /db_xref="GI:256005616" /db_xref="InterPro:IPR002545" /translation="MPEILTNELELQQEETQRDKYLTFIVEKEYYGIEIRYVTEIIGI QSITEVPDLPKYIRGIINLRGKIIPVMDVRLRFGKEFREYDDRTCIIVIDVDNMFVGL IVDSVCEVMSIPDGEIVVPPDFDQSENKFIKGIGKTGNEVRLILDCEKLLKD" misc_feature 36525..36929 /locus_tag="ClothDRAFT_2436" /note="CheW, a small regulator protein, unique to the chemotaxis signalling in prokaryotes and archea. CheW interacts with the histidine kinase CheA, most likely with the related regulatory domain of CheA. CheW is proposed to form signalling arrays together...; Region: CheW; cd00732" /db_xref="CDD:29682" misc_feature order(36651..36668,36750..36752,36756..36758,36912..36929) /locus_tag="ClothDRAFT_2436" /note="putative CheA interaction surface; other site" /db_xref="CDD:29682" gene 36985..>38231 /locus_tag="ClothDRAFT_2437" CDS 36985..>38231 /locus_tag="ClothDRAFT_2437" /inference="protein motif:PFAM:PF00672" /note="PFAM: histidine kinase HAMP region domain protein; SMART: histidine kinase HAMP region domain protein; KEGG: ank:AnaeK_2477 methyl-accepting chemotaxis sensory transducer" /codon_start=1 /transl_table=11 /product="methyl-accepting chemotaxis sensory transducer" /protein_id="ZP_05430576.1" /db_xref="GI:256005617" /db_xref="InterPro:IPR003660" /db_xref="InterPro:IPR004089" /translation="MLRWYYNLKISAKLIIGFLLLALVAGVVGVVALSNINNMSQADA ELYEKNTLGINYAAGASLRFQRMRYNTAKLLVYDAEQVSKGIEKIQEHVENTEKYLSL YESTVINETDRIQLQELKTLWEKYKSLVDKEVELVKSGKTEEARQLLLSDIDDIGDTL RDYFEAFVEYNTTAAKEKVDENKQVASTASTVMIVVIFVGILIAIALGVFISRIISKP IGQMVEAADRLALGDVEVDVKAETRDELGKLAESFRRMIENIREQAYVVEKIAEGDMT VDVRVKSDKDLLGKKLKEMVEINNEVLSNINEVAAQVAAGAKQVSDSSMQLSQGATEQ ASSIEELTASLEQVANQTQISAKNANQANELAEIAKNNAEQGNKQMAEMLNAMEEINN SSSNISKIIKVIDEIAFQTNILA" sig_peptide 36985..37083 /locus_tag="ClothDRAFT_2437" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.998) with cleavage site probability 0.594 at residue 33" misc_feature <37333..>38231 /locus_tag="ClothDRAFT_2437" /note="methyl-accepting chemotaxis protein I; Provisional; Region: PRK15041" /db_xref="CDD:185001" misc_feature 37627..37770 /locus_tag="ClothDRAFT_2437" /note="Methyl-accepting protein, and Phosphatase (HAMP) domain. HAMP is a signaling domain which occurs in a wide variety of signaling proteins, many of which are bacterial. The HAMP domain consists of two alpha helices connected by an extended linker. The...; Region: HAMP; cd06225" /db_xref="CDD:100122" misc_feature order(37627..37632,37639..37644,37648..37653,37660..37665, 37669..37671,37717..37722,37726..37731,37738..37743, 37747..37752,37759..37764) /locus_tag="ClothDRAFT_2437" /note="dimerization interface; other site" /db_xref="CDD:100122" misc_feature 38062..>38231 /locus_tag="ClothDRAFT_2437" /note="Taxis toward Aspartate and Related amino acids and Homologs (TarH). The Tar chemoreceptor of Escherichia coli mediates attractant responses to aspartate, maltose, and phenol, repellent responses to Ni2+ and Co2+, and thermoresponses. These...; Region: TarH; cl00144" /db_xref="CDD:153541" ORIGIN 1 ctaaatcaaa gatattgtgt tacaggtttt tagaaaacag tgaggtgagg cgttttgaga 61 tatgtgagta tgatattaaa tctaaaaaat ttggtgaacc tatttttatt cccggcgatt 121 cacagaagtt tgaaggtagt gttaaatacg tgcccaattc cgacagaatt agttttgttt 181 tagattatga actacatatt tatgataggt cctcagggac tcttactaaa ttatttgagg 241 tggcatttaa ttcgtactca tgggataata cgggcaaaaa gttactttat agtgattacg 301 atgaaaacat atatatatat gatatggaga gcaatgaaaa gagaaaaatt ttaacaggta 361 tatgccccgt ttactcaagc agcaatgaat atatagcata tataggaata gatcagaggt 421 taactgttta taatgttaac acaggaaaaa aatggagaac cgtaccagtt gacgatagtg 481 caagatatat tttttcccca gatgacaagt atattctatt gggaacagaa tatgcagacc 541 ttatgagttt tccgcattac acattgtata ttttggatta taagacagga aagaagaaga 601 gattatttgg cggtgaagga aatgttccca gtcttgattg gaaataggaa gtttgggtaa 661 tagttcttct tcaattattt gttcatgtta aaatttacct attagcgagg gtaatattta 721 taaagaaaaa ccagaatgtg cattcatgcg gagatcgaat tccaactggc agctaatgat 781 gagactaata caaacactat agcttttatt ttaaattatt atgcaaggat tatccaattc 841 ttatgttgat aaatattggg gtaaacgaac ttattatgta ggagaaatca atatgacatt 901 agatgaaaag ttgagatatg gtataaagat gataaaaaag ataaaaatgg tgtaccaaac 961 ataattacgg tagaaattga tacagagaaa aataaaattt tacgtattat taatcagaaa 1021 cgaaattcga aaatcgttcc gggcaatatc aatatagata aatggtctgt agatagtata 1081 gatgttattg atatggccag gaaagtgttt gcgcaaaacg atgcttttag ttttaccaca 1141 gcttggttgt ctgggactag tactgataaa aacggtaaag agatttggaa tgctaccttt 1201 tataatgaaa atactaaaaa ggcatattat ttgcaagttg atgtttatat tttatttcat 1261 ataaagattt gatatttgaa gatatttaaa actaagtatg agaatttatt gaagttgaaa 1321 gtagttgatg ttataatttg tgtgattaac aataaattgt tttcaaaagt gcaagaataa 1381 ctataaaaaa ttatttgatt agatattaga taaagaaaaa atggccaaca aatgatatat 1441 tgctatcttt aacggtagca tatggcacaa gtggatttga caaaaactac gagaagttaa 1501 aatacttgaa attttaaaac aaatggattt tgactttgcg cagttctcta ctataaaaaa 1561 gaaaacatta gggggaatga aaatagtgag tttcttaaaa aagcatgtac cggctttagt 1621 gcttatttta atactcttaa taggtgcaac ggctttattt ttttatataa atgagaaaga 1681 aaaacaggac tatattgaaa tattccacag aaataaaagc atttttgaag aaatatcaac 1741 tgaacttatg ccgttggaat atggaactac tgtttcgaaa gaggcaggca aaattgttgt 1801 aaaacaaaac gggaatatca cagaaatttt tcaatatcca attgatgaag aaacaaaaac 1861 aaatataaga agagcgataa atgatttcgg tgtaaaaagt attaataaaa cagatgaatg 1921 cttgaagttt atctacagca gcaaaaggga ttttcatgct ttggtttata ctgcggataa 1981 aagtaaattg accggcttta ccgatgtgga acacttagag ggcaactggt actattgtta 2041 tatactttat gaataattat gtggtgcaaa gggagatatt tgcggtggca aaacgcgcgg 2101 gaaaatgttt actgtttggt attattgggg tattggcagt tgcggtaatt tcattaaaat 2161 gatgaatggg ccgggtattg aattgggaaa gattttgatt tgacttcttt acaatattgt 2221 tatatctgaa ggaagagaaa taattgaaat gaaatataaa cgtgaaaaaa catttcattt 2281 tagactgaca aagtatgcaa agtcagtttt gggaagcgaa ctctactcca ataaaatatt 2341 tattcacaag attgataagg aaaacaaggt atataatcaa gatgtggtac agctaatgaa 2401 acggctttac ggaattgata tggagaaaag ccagtatccg aaactgcgct gtgaaatacc 2461 gccggttgat attgttatta caatgggctg caatatgaaa tattccggag aaatatccgg 2521 aggaatttta ttgaaattgg aaagtggcta atggtataat ttacataata gattcatttc 2581 aaatccgttt tggtaagtag ggggctaaag acgggtatgc aaataaaaat aacacctgaa 2641 gaaatgacaa ggatagcggg aaatataaga gaagtttcag ataagtttaa tgatatagca 2701 ctggaagtaa aaaggataat aaattctatt gattgggagc ttagaagcag gaagggaata 2761 aacgaaaaat cgtcaatagc gcaagcaacg gcaataaaaa tatccgatag actgaataaa 2821 atgtcacaga gccttataag ggcaagagac aagatgatag aggctgacaa taaggcttca 2881 gccacagcca aaaagatgaa gactgtaagc tttaaaagga cggttgctgc agcaacgttg 2941 tcaaattatc cgtcagatgt agaatatgtt gatccgttgt ggaaccatgt tgtaggtccc 3001 ggaactgcaa actgccctaa tacatatgtt ggagacccta taaatgtggc atcgggaaat 3061 ttctatttaa caaggagaga tatagctata ccttcaaggg ggatggggct tgaaataacc 3121 agatattata attcgatgga taatacgcaa ggcatattcg gtaaaggttg gaggatagat 3181 tatgaaacgt gccttaagaa gaaggaagat agtgaagaca taatagtagt atacccggaa 3241 ggaaatataa gagtatttga gtataccgat acaggagatt tcaaatctcc caagggtgtg 3301 tacgacacgc tttttaaggc ggaagacggt acatacatat taaaagttca aaaaggaatt 3361 acatacaaat atgaccaggc aggaagcctt atatcaattt ccgattcaaa taacaagaga 3421 tacaactcaa atacaatcgc gagggattgc tgtctacggt aatatcaaca cgcagaaaac 3481 ttttgatgtt ttcttatgaa ggctacaggg ttagcagcat aactgatcat acgggaagga 3541 agctgaaata taaatacgat gaaaaaggga atctgacaca ggtaatatac cctgacggag 3601 ggaaaattac ctatgcctac gatgacgtag ggttaatttc agtaaccgac caaaacggca 3661 atacctatgt ccaaaacacc tatgataaaa acggaagggt aataaagcag cttgaccatc 3721 aggagaatga attgattata gaatatgacg atgagaatcg tgaaaacacc ttcaaatggt 3781 taaagagcgg tataacccgt gtatacaaat acaacacgga catgcttgtt actgaagtaa 3841 gatatgatga tggaagtgtg cagaaataca cctatgatga aaatcttaac agaaacagcg 3901 agaccgacag aaacggtaac acaacatgca aaaaatatga tgataagggc aatttaatag 3961 aagtaacctc accggaacct ttctgctaca aaacaaagta cagctatgac gagaatggca 4021 gactgataaa aacagtgtca ccgggcggcg gagaagtaac atttgaatat gatgataggg 4081 gcaatctttt aaaacgtacc ttaaaaaccg gaagcagaag ttattcggag tggtcgtata 4141 cctatgacca atacggtagg ataacaagtt caagagatgc tgagaacaat ataacaagct 4201 ttgagtatgg agaagaagac gtaaacaagc caacattaat aaaagatgcg gtggggaata 4261 cattcagata tgaatttgac aaagttggac gagtgacagc aataacgaca ggttatggca 4321 cagtgaggtt aagatatgat gactgcgacc ggataaccca cataaccgat gcggaaggaa 4381 atattataag acttaattat gataaagcag gaaatatgac aaagattata gcgccgaagc 4441 agtatgctga aaaaggcgaa aacggggcag gatatacttt tgaatatgat gcaatggata 4501 agctgataaa gactattgac ccattgggta atgtctttgc ggtaaaatat gatgaaaacg 4561 gtaacaagat aaaagaaatc aacccgaact actataattc tcagaaagat gacggtatag 4621 gaatagaata caaatacgac accaaccacc gcaggataag cacaatattc ccggacggaa 4681 gcatgtcaag aacgaaatat gaccctcagg gcaatataat aaagactata tcatggaagg 4741 actatgacaa ggatttggat gacggaccgg gaatggagta tacttatgat gaaatgaaca 4801 gacttacgca aataatagac cctgaaggaa atgtaataaa gaaatacata tatgacgaag 4861 agggaaggat cgtaaaggaa atagatgcaa aaggctacag cagtgcagat aatgacgaag 4921 aacgttgggg aacaatatat aaatacaacc ttgccggctg gcttgttgaa aagaggacac 4981 cgctacggca gaaagacggc gaaatatact ataacataat agaatatgtt catgataaaa 5041 acggaagagt agtgaaggag aaaagatctc cggaatatgt gaccaaatcg gaatatccga 5101 agaaatggaa cgtaataaac tacaaatatg atccgaacgg gaatttaata gaggtaacag 5161 acagccttgg agcctctata acctatgagt atgacagcct tggaaaaaag actttggaaa 5221 agacgaaaat aaacgataaa aaacaaaaag taacgcgata tgtgtataac agtgtgggaa 5281 gactggtaaa agtaatacaa gagctggatg gagaagacct ttcaggctac agtaatgaga 5341 aggttttagc agagacaata tacaattacg atccaaacgg caatcttata gaagtaatct 5401 ctccggaagg atatcttaca gtatttaaat acgatgatgc aaaccgtagg ataaagagta 5461 tattgtatca accgcaaaac ggtgtgaaac tgatcggcag tgcgtattgt gcccttttaa 5521 atacaaaggc gaggagcata agctatgagt atgaccgggc agggaacctt gtaaaacaga 5581 tattgcctaa cggtggcgtc ataataaacg aatatgacga gatgaacaga aagataaaag 5641 ttactgatcc tgacggaaac accacaagaa ttttctatga caatacagga aacattgtaa 5701 aatatgttga gccgggaaat tacaatccgg agaaagatga cggagtgggt actacatatc 5761 tttatgactc aatgaaccgt cttatacaga tagtaaatgc tgcaggaata gtggtggaaa 5821 agaatatata caatactgcg ggagaaataa tcaagaaaat tgactcagta ggttataaat 5881 ccgcagataa tgacaatgac aggcatggag ttgaatttag ttatgacctg gcaggacgtt 5941 tggtggagat aacaacgccg gaagcgaaga ttcacggacg aaagagccag caatacactt 6001 atgacgcaga aggaaacata acgggaatag ttgacggaaa cggaaacagc acaaggtaca 6061 gtttggactt atggggtaag ataataaaca taacggaacc tgacggaacc aatataaaat 6121 acgattacga ctatgcggga aaccttgtgt ccactactga cggtaaccga aacaccactc 6181 ggtatacata caacagcttg aaccttctgt cggagataat agaccctgac ggaaggaaaa 6241 taaccttcaa gtatgacaga cagggaagaa tggtgcaaag gatagggaaa gacggacaca 6301 gcacatatta cacctacaat gcggacaaca atataaccgg ctgttgggaa gaagaaggac 6361 agatggagag gtacgagtat aatattgacg gaagtctggc tgcgtcaata agcggtacca 6421 ctatacatac ttatacctat accttggcag ggagactgaa aagtaagaca accaacgggc 6481 ggaaagtgtt ggagtatgat tataataaaa atgggtttgt atccaggctt gttgacataa 6541 gtggaacacc ggtggagtat acatatgacg tgctcggaag agtagctgcg ataacaaatg 6601 gaggaaagat ttccgcaaaa tatgaataca atattgacaa cacgatagcg caggtcttgt 6661 atgggagcgg aatatgcgcg agatatgaat atgacctgga taagaatata acgaggcttt 6721 tgaatacaga cccgacagga aaagagatgt ttgtgtacaa atatgcctat gacggaaatg 6781 gaaatccgat tttgaaggaa gagaacggaa aagtaacggc ctacggctat gatgcactaa 6841 accgcttgca ggaagtgata taccctggaa atgtaaaaga gagatttacg tatgatgcga 6901 acggcaacag gctcaagaga gagttcagag acatattgga gcaatatgag tatgataatt 6961 gcaatagatt gattcaaagg ataaaaaatg gactggtaac ggaatatgaa tatgacgaaa 7021 aaggaaattt gataaaagaa aaagaaggtg agttgactaa attatacagc tatgacggat 7081 ttgacagact gatacgtgtg cagaatcccg acggaacata tatggagaat atatatgatg 7141 ccgaggattt gagaacgata tcgatagaaa acggaagata caacagatat atatacagcg 7201 gaagaaatat agcgtgtgaa gtagacgagg actggagact gaaagacaga atagtgcgag 7261 ggcatacgat attgcaaaaa gaggacggca ataagaatgc ttactattat attcacaatt 7321 ctcatggaga tattaccgct cttactgatg ataaagggaa aatagcaaac gcttacagtt 7381 atgatgcttt tggaaatata ttggacagtg ttgagagagt agataataga ttcaagtatt 7441 caggcgaagt gtatgattct gtagcaggtc aatattacct gagggcaaga tactataatc 7501 cgagtatagg gagatttata caggaagacc cattcagagg cgatggactt aacttatatg 7561 cctatgttgc caataatcct gtaaggtaca ttgacccgac cggtaactgc aaagaaggtg 7621 ttaactttaa taacaacctt gaggaaacga caaaagtgat tgttgaaaaa aatgaaagta 7681 aaggcaattc ggtttgggat atagtaaagg atatttttgt agatacaatt gagggtgctg 7741 tagacgagat aatagcaaag aaagttattg agagattacc taattattca gcaaaaacat 7801 tgatggggca tgcaaaattt ggttatgctt ttgaggcaac aaaggttaaa cctatacctg 7861 gggttaaggg actaaaatca ttttcaaaag ttgcaggccc tgttgcagtt atggtgtatg 7921 gatacgaagt ttatcaggat ataaaaaaat acgatggctg ggatgcggtt aaggctatta 7981 cggtaactac agtagcaacg gcagtaacat ttttggctgg ctttatttta gcacaagctg 8041 ctcttccggt agttgcaact cttattgtga gtgctgcagt gggtattgct attggatttt 8101 ttgcagattg ggttaagaaa aaatggatag gttattaatt tattaggaat tgaggaagga 8161 gaaacaatta tgagtcttaa ttatttgccg ttaccggtct atgtgagttt atgtttattt 8221 cttagtatag cgcttatact gatcgcggta aaacgacgga atgtagatag aaaagggttg 8281 ctggttcttt atttaatcgg ttcagcaggt ggaatacttg caacaataat aagaattctt 8341 aaggaaagaa ccagcatcta tgatgattat tttaatcaga ttggctatat tttgtttgga 8401 tatataatat tattatttgc tgacatattt tatatatcta tgactcaaaa agtagacaaa 8461 gaatctagaa aaaaaatatt aataggttgg gtaatgattg ctgtaagtat tctggcaggt 8521 gtagcagtct attttatact tgattaaaaa gtgaaattat ttcatatagc aagcaataaa 8581 aagcaaatta tagaaattta aatatcctat ttactcaaat gaccttatct aaattacagg 8641 catattttgt tagtaacaca atattgatac atttgcggat tgtaagacgg gagaaattat 8701 ttaatgatga tggcgtaata cctggttgta attggaaaat ctcggataaa tttaatgata 8761 tagcgcggga agtaaaaaga tagtaaattc tattgattgg aagcttagaa gcaggaagga 8821 aataaatgaa aaatcgtcaa tagcgcaggc aacggcaata gagatatcca atgaactgaa 8881 taaaatgtca cagagcctta taagggcaag agacaaaatg atagaggctg acaataaggc 8941 ttcaaccaca gcgaaaggga tgaagactgc aagctttaaa acaacggttg ttgcagcaac 9001 attgtcaaat tatttgtcag ttgtggaata tattgatccg ttgagaaacc atatcgtagg 9061 ttccggaact gcaaactgtg ctaatacata tgttggggac cctgtaaatg cggtgtcggg 9121 gaatttctat ttaacaagaa gagatatagc cataccttaa agaggaatag ggcttgaaat 9181 aaccagatat tataattcca tggataatac acagagctaa aaattggaga atagattatg 9241 aaacctgcct taagaagaaa gaggacagtg aagacataat actggtatat ccggatggga 9301 atataagagt atttgagcgt accgagtcag gaacttttaa atctcccaag ggtgtgtacg 9361 atatgctttt taagacaaag gacggtacat acatattaaa ggttaaaaaa ggaattacat 9421 acaaatatga tcaagcagga agccttatat caatttccga tccaaataac aatgagatac 9481 aacttaaata caatcgtgag ggattgctgt ctactgtaat gctaacgggc ggaaaacttt 9541 taatgttttc atagtagcag gtgttgctat tggaatgctt gcggattggg ttaagaaaga 9601 atggataggc tattaatttt ttgaaaagcg agcagggaga aataattatg agtcttaatt 9661 ttttaccgtt acaagtttat gtaagcttat gtttatttct tagtatagcg cttatactga 9721 ttgcagtaaa acggcggaac atagacagaa aaggattgct ggttttctat tttatcagtt 9781 cagcaggtgg aatacttgca gcaataataa gagttcttaa agagacaacc agtatctatg 9841 atgattattt taatcagatt ggctatattt tgtttggata tgcaggatta ttttttgctg 9901 atttatttta tttatctgta actcaagtga tagctaaagg agacaaagaa gcaaaaagaa 9961 aactaataat aggctgagga atgttttttg taagtatttt aatgggggta gtagcttatt 10021 ttatacttga ttaaaaaacg gaaaatgtat atcgagaata tataataaaa aatatataaa 10081 atataaataa aaaactgaga ttgtgaaaag aaaaagcggg ctgattagaa gagaacaagt 10141 aattatgagt cttaattatt tgccgttacc ggtttatgta agcttttgtc tgtttctcag 10201 tatagtgctt atctcttttt ttatttactt tgttccggaa ttggaatact tgcagcaaga 10261 gtaagaattc ttaaggaaac aaccagcatt tataatgatt attctaatca aattggttat 10321 atttcgtttg gatatatggg actggtattt gctgatttat tttatttgtc tgttactcaa 10381 acaatagcta aggaagacag agaatctaaa aaaattcata ataagttggg gaacacttgc 10441 tgcatgtatt atagcagatg tagcagtcta tttcctactt gactgaaact caaaaaaaca 10501 agtaaaaccc actccaaaaa acatttaatt ctcgtattat tataacacaa cactggttta 10561 ataaattaaa agagataatg gaggggattg gctgtggcaa acaggacttt taaattcatt 10621 gtattgcttt ttactacagt attgtttgca acttcagtgt gttactcaac taacccgata 10681 acaaaagcaa aatttcagtc ctataattat cctaatatgt acataagaca tgcaaatttt 10741 gatgcaagga tagacgagaa tgtcacgccg gaaatggatt cccagtggga gttggttccg 10801 ggtcttgcca atagcggaga tgggtatgtt tctatccaat cggtaaatta tcccgggtat 10861 tacttaaggc attcaaatta tgatttaagc ctggagaaaa acgacggaac ttcacttttt 10921 gcggaaagtg caacatttaa aatagtaccc ggacttgcgg atccttcata tatttctttt 10981 cagtcctaca atttccccac aagatatata aggcattaca attacttatt aagattggat 11041 gaaattgtga cagagcttga cagacaggat gcaaccttta aaataatcag cgaggatact 11101 caaccacctg caccgacact cataggtgat gtaaatgccg atggaaagat tgactctaca 11161 gaccttacat tattaaaaag gtatttgcta agaagtgcaa cgctcacaga agagaaaatt 11221 ttaaatgcgg atacagacgg aaacggcacg gttaattcaa ctgacttaaa ttatctgaaa 11281 aaatacatat taagggttat atctgtattt ccggcagagg gcaataagcc cccgactccg 11341 actccaacaa aaacacctgt ggcaactccg tcaccaacac aaccactgtt tacacccagc 11401 tttaaagatg taacggtgca cgacccttcg gtgataaaaa ccaacggaac ttactatgtt 11461 atcggttcgc accttgcctt tgccaaaacc aatgacctta tacagtggca gcaaataaac 11521 tccagtgttt atacaggcaa tcctttaata ccgaatgtat ttgatgagct gaaagaagct 11581 tttgaatggg caaaaaccga tacaatgtgg gcgggggata taattcagct tcgggacggc 11641 aagtattaca tgtactattg tatgtgcaaa ggagattcgc cgttatctgc ccttggtgtt 11701 gccagagctg ataaagtgga aggtccttat aagaatttgg gaattctgtt gaggtccggc 11761 gcaggaaaag gtgaggacgg aacaaattat aatgctacga tacatcctaa tgcagtcgat 11821 ccccacacct tcttcgataa aaacggtgat ttgtggatgg tttatggttc ttattcgggt 11881 ggaatttata ttttgaagat gaatccttca acaggtaaac aatatcccgg gcagggctac 11941 ggaaagaaat tattgggagc aaatcacagc aggattgaag gcccgtatat acaatacagt 12001 ccggataccg gttattatta cctgttctta agtttcggag gcttaactgc tgacggcggc 12061 tataatatca gggttgccag atcccaaaat cctgacgggc cttattatga cgctgagggg 12121 aataatatga ttaattgccg cgggccggca ggatctttct ttgacgacag agctatcgaa 12181 ccttatggtg ttaaactgat gggcaatttc cggtttgttg acagcggcat tggctatgtt 12241 tcaccgggac ataattcaac atattatgat gaagagtcgg gaaaatattt tattattttc 12301 cacacccgtt tcccgggcaa gggagagatg catcaggtga gagtacacca aatgtttatt 12361 aacgaagacg gatggcctgt aatagcaccc caccgttatg cgggagaaac cataagacgt 12421 tattccccgg aggaagttgc cggcacttat gcctatataa atcatggcag ggatatatca 12481 gccaatataa aaaactctgt aagaatatcc ctgaacaaag acggaaccgt tagcggaaac 12541 ggcgtaaacg gaagatggga attaagcggt gaacattata tcagattaac tgttaacggt 12601 acgaattata cgggcgttgt attgcagcag tgggatgatg ggcttaaaaa gtatgtgatg 12661 acctttagtg caatatctag gaatggaaat gtcacaattt ggggcagcca actgtagtct 12721 gcaaagaaat aattatttca attgttttat attaaataat aagctttggc atgattacac 12781 ttcttgccga agcttattta ttttttactt tatataaatt tctactttgg caggcaatgc 12841 aaaaaaggcg tcccaccgaa ttttggaagt aatgcctcaa ggtacagata tatatgactt 12901 atatatatga cttaaaaggt ttgaaaagca tcctctatat ttgtgccatg tctgaaaatt 12961 tgttttgaat gtatgaatat gtggtatata aatataaata gctttgttaa taaaatatat 13021 tctatattgc aataatagta gatgaaagat acaaacaaag gataccggga ggatgaaaaa 13081 atgagcaagt tgtatgacaa tttgaatgaa caaatgaatt ttgagctgga gtctgcatac 13141 atatatgcaa ctatgtctgc gtatttggat tcccttaaca tgaagggtat gacccatttt 13201 ttcgatgagc aggtaaaaga agaaatggag catgcgggaa aaatcaaaaa atttctgcag 13261 gacacaggat acggcataaa atatcgtcct ctgaaccctg gagacggcaa atttgattcc 13321 attgaagatg tgttcaaaaa agctcttgcc catgaaaaaa cggtaacttc ccgtataaat 13381 gatttggtaa aacaagcaag agaagaagga gaccagaggg tgctcaatct tttggcgtgg 13441 tttgtggatg agcagattga ggaagaaaac atgtttggag tacttgtgga aagactggaa 13501 agaatcaatg gacactggaa cggcttgtat attcttgaca aagagcttgg agaaagataa 13561 aataaaaata taaaaagatt ataaaggcaa agacgctggg agattatcac tggtgttctt 13621 tgccttttaa agttttagtg aatgcataat gcacgtattt taaaattatg atataatata 13681 taatataatt aaggaagaaa tctgtgattt taattatgga tgcttttcaa tgtaaaaaat 13741 ttttgaaaaa ttcatttgag aaattgctaa aactggcttt acatttttaa aaagtcaaga 13801 attatttaat tctattattt aattttaaga agcatggttt ttcaaaaaca aggataaaac 13861 aagtcttacg aaccttttaa tcttaatatt taacggtaga ttaaaaaaag taaagacgcg 13921 cgtaaagaat tacagataaa aatacgcagg ggtctttctt tgtcaacaag tgcaaacggg 13981 gtgaaagcga ttttgatttt aatgctgaaa gtgccgaaaa agaaaaaatc gaaataaaaa 14041 aattgtaatt atgtctcaag gggaattaaa ttggtaattt atatatttta attaattttt 14101 tagatgggag atagagatga agaaattaaa tttgaaaagt aaactcgcca tttttgccac 14161 aacgttaaaa gaagttttta tatcttcgct gcctcttgcg gcaattatga ttattgtgtg 14221 cggttttatc gcacctttgg acagtggggc ggagtatgtc aaattatttg tcggctatgc 14281 cagtgttgtt tttggccagg cattgttttt ggacggttta aatattagta ttcttcccat 14341 aggaaaattg gttgggggtt cgctaataaa gcttaaaaaa tcaatctttg ttattttctt 14401 cggacttctt tttggcgtgc ttgccactgt cgcggaacct gcactgaccg ttttggccaa 14461 gcagaccaac atgattatgc cgattatcaa cgaaaccgtg tttatctgga ttatgggttt 14521 tggaatcggc gtgatggttg cattctccct ctttcgaatt atgaaggact taaatattaa 14581 agtggttttt gccatattgt atgtcattac ttttctgttg attatatttg ttcctgatga 14641 atttgttgct ttggcttttg acggaagcgg tgcaactacg ggggacattt cggttccgtt 14701 tattttggct ttgggtatgg gtgtttccac taccatgtcc aggcacaaaa gcaatgacga 14761 cagctttggg attattggcc ttgcttcggt gggtccgatt atttcattgg ccatctacgg 14821 tatagtactt aagtttcttt acaacggtgt atttcctcct gaacaggtat actctcctga 14881 gacggtggga accgttggtg agataattgt aaataatttg tggggagtta cattggcact 14941 tttgccggtt atcatagtgt ttttaccgtt tcagtttttg ttaatcaaac agccgaagaa 15001 agaatttgtg aaaattttgt taggtactgt tgtagttttt ataggcttgc tgatttttct 15061 ggcaggcata gattatggat ttgcatttgc aggcaaatac atcggggagg ttttcctgga 15121 tccttcacgt cccgagtggt ttaagtggtt gcttttgatt gttgcgttta ttttaggtgc 15181 cgccattacc ttgtcggagc ctgctgttac ggtgctggga gaacagttgg aagagatgac 15241 caacggacat attgcaaaga tgacaattcg catgactctt gccataggta ttggctttgc 15301 tgctttgctt ggaatgttga aaatattgac ggagattaac atattgtggt tcttaatacc 15361 cctgtacgcc gtcgctctta tcatgatgat atttgcgcca aagctgtttg tcggtcttgc 15421 ttttgactcg ggaggagtgt ccggcggagc tttaacttct gcatttttga cgccgcttac 15481 ccttggggtg gcacaggctg tggctgcgac atcaccttcc ggcggacagc cgattttggt 15541 caacggtttt ggaattattg catttatttc agttacccca ttgattgctg tacaattttt 15601 aggtatagtg tataatataa atattaagaa agcggaaaaa gctctgaaag atgctgaaat 15661 gaatgatata aaagagttgg cgtcccttgc gggtatcgtt gaagaagtcg cagctgaaaa 15721 aagtgcgact caagaaagta tagctgagaa agctgcagtt caggaaagta tagttgaaaa 15781 aggtgggatt gaaaaaagtg tagtggatga agaaagtata accgaaaaaa gcatagttaa 15841 ggaaaatata gatgaagacg atatagcggc taaaatggat gaacagcgtc aaaatgagca 15901 gcatcatgaa gataatattg tgaaggacat gaaagacgga cacgaggaac ttaaggcagg 15961 taaaaacagt gcggagtaga ttattttaga ggaggaaatt attgccttga cggaatataa 16021 acatggtgtt gactttttaa cattaatagc ggatagaaag tataagaagg aaatcttaaa 16081 atttctgttt gattttcgat gtcatttaat tgatgtgatt tatgtaaaag gtacggtgaa 16141 atccgaatat ttcagggata tgctggggct tgttaacgag gagaaaaaag ttatgattac 16201 cggcttgatt gcgggtaatt tatccgagca gttacttgag gagttggtca caaaattgaa 16261 ttttgacaaa ccaaatacgg ggattgcctt tgttgtccct gtgaataagt tatcagtatg 16321 atttgggagg attaagatgg aaagcactaa tatgaaagca ttgtatattg ttgtcaatgc 16381 gggatttgcg gaacaagtgg ttgattatat acgttcccag ggttctaccg gggccactat 16441 aattaatgcc cgcggagtca gtgcgcttca caaagaaatt atgggcatta gtgtggacac 16501 ggagaaagaa gtggtactga ctgttactga aagtgaaatt gccgacaaaa ttatggaggc 16561 aataaagcta aatactgctt ttagaactga agcccagggt atttgtttta ctttaccggt 16621 atcgaaagcg gttggcttga gaaaaaggtc ctcatcggag taagatggta ttgagtgttc 16681 tcagatgtcg gcaaatgaca gcaggttggg gattttttgc aagaaagttt cccagcctga 16741 ttttattgtt tgattgtcaa ccttgaatta aaataaagtt gacaattaat ttttatatgg 16801 atataatgta aaatggaggt tggaaatgga gactgaaaat gggagatgaa ggtttgcatg 16861 gattttttaa cggtgatgga aaataaattg gacgaaggcc ggatgattac ctttgaagag 16921 gcagtagagc ttgcaaaagg gaaaattgag gatgaaaaac tgtttttact ggcagataag 16981 ctttgcaaaa agaatatggg ccgaagagtg gacctttgct cgataattaa cgcgaaatcg 17041 ggaggatgtt cggagaactg caagttctgt gcccagtcgg ggcattacga tacaggagtt 17101 aaaatttacc ctttgcttga cgttgacgat gtgttaaaag cggcaaagga aaatgaaaag 17161 gaaggtgtgc accgattttc gcttgtaacc agcgggaaaa gcgtttcaga tgaggaattt 17221 gaaaagattc ttggaatata ttcggtatta agaaaagaga caaatttaaa gctttgtgct 17281 tcattgggaa gtctaagtta tgagcgggcg gtaaggctta aagaagcggg ggtatccatg 17341 taccaccata atattgaaac ttgcagggag tattacccta aaatttgtga tacccataca 17401 tatgacgaca gaataaacac cgtaaagaat gcggcaaagg caggtctgga aatttgctgc 17461 ggcggaataa tcggcatggg ggagagcatg gaacaaagaa tcaagatggc gtttgaaata 17521 agggagcttg gcgtaaaatc ggttccaatc aatgtattaa accccataaa gggtacacct 17581 ttcgagaatg tcagaagtct ttctcccgat gagatactca gaactatagc ccttttcaga 17641 ttgataatgc cctatgccca cattagatat gccggaggga ggatgtgttt gggagaacat 17701 cagtccaaag gctttaaagc cggcgtcagc gcaatgctgg tgggaaacta tcttacaacg 17761 gtgggcaata aaatttcgga cgaccttgaa atgattcaaa gaatggggct tgaaatatga 17821 atggtaccaa aggaatatat attatcggca cagatacaga tgtgggaaag accgttgtct 17881 gtgcagggct tatgtattta ttgaagtcaa aaggatatga ctgctgctat tttaagcccg 17941 tatccagcgg cggcaggcaa acggaaaacg gctttttctc ttatgatgtt tcttttgtaa 18001 aagaagtctc ggggtttaat gaagatgatg agatgataaa tccctttaga tttaaaacgc 18061 cggtttcacc gcatctggca tctgaggcgg aaggttttgc agttgataag gaaatcatcc 18121 tggataggta cagaaagctt agtgaaaagt accggtatat cgtggctgaa ggatgcggag 18181 ggcttgcggt gcctttgtcc agggacggat atatgcagtt tcagctgatt aaagaaatgg 18241 ggctttcttg catcctggtt tcccgaactg tcttgggaac gataaaccac actttgctta 18301 ctctctcctt tgcccaaaat gccggtattc caataaaggg aataattttt agcggttttt 18361 cggatcggac tttagagcgg gacaatatag agacaataag aaagttgtcc aaagtcccgg 18421 tgcttggggt tgttcccaaa atagatggaa ttagtgttga aatcattaat gctgaggaca 18481 agattttagg agaactgaaa tccgttttcg aaaaaacaat aaatatagaa gagttgttgg 18541 caaatgtatg attttcaagg tgctttgaaa gatataaaaa acaagggatt atacagggag 18601 tttcgaaatg ttaatgctgc ccagggcccg tataccgtta ttgacggaag aaaaatgctt 18661 atgatgtcat caaataatta cctgggcttg tgtgatgata taaggcttaa aagagctgcg 18721 atagaatcta ttcgtaaatt tggtgtggga gccggaggct caaggctgac ttgcggaaac 18781 tttgagcttc acagggagct ggaggagagg cttgcaaaat ttaaggatgt ggaaagctgt 18841 attgtttttg gaagcggata tgccgcaaat ataggagcaa tatcgggaat tgcggacaaa 18901 aactgggtca tattctgcga ccgtctgaac catgccagca ttgtggacgg cattcgccta 18961 agcggtgcaa aacttgtggt gtataaacac tgcgacatgg aggaccttga aagcaagatt 19021 gtacgctatc ataccggcaa aagccttata gtaacggatg gcgtgttcag catggacggg 19081 gatgtggcac cggtggatag gattgtgaag ttggctaaaa aatacaatct tatgacaatg 19141 gtggatgatg cccatgccac aggaattttg ggagaaaagg gaagggggac gtcggagtac 19201 tttggcctta aagatgctgt tgatataagc atgggtactt tgagcaaggc ttttggtgtt 19261 gaagggggat ttgttgcagg aaagagaaag cttgttgatt ttttacggca caaggccaaa 19321 agctttattt actctactgc tccgccgcct tataatatgg ctgcggcgtt agaagctttg 19381 aatatcatag aaacggagcc gcaggcaaga aaggaattgg ctgaaaaatc cgtgtggcta 19441 agaaacaggc ttatagaaaa aggttttaac gtgcccaaag gggtgacgcc gataataccg 19501 cttatggtgg gagatgtaaa tactgcagta gagtttagta tgctgcttta taacgaaggg 19561 atatatattc ctgccatcag gccgccaaca gttcctaaag gaacgagcag gcttagaatt 19621 tccataatgg cttcccattc ctatgaagac atggagtttg cccttaaaaa ccttgtccgg 19681 ttcggaagga agttggggat aataccataa tgttgacagg ggaggataat ggtttggaaa 19741 aacaaaagct tgtcatgatg cccggatgga gtatggattc ttccgtatgg aagcccgtcg 19801 ttgcggcttt aagtgagtat tttcagctta ttttttgtga atggcatggc ataactggca 19861 tggaaggcta tactgaaagg gtatgtaagt taattgagaa ggagaaagat aaattatgtc 19921 ttctgggatg gtctttggga agtttgcttg cgattgacgc tgccggcaaa tacaaagaca 19981 gggttgaaaa acttgtcttg gtaggcggta cggcgcggtt tacaagggat aaagagaccg 20041 ggtacttttg cggctggcat aaaagtgcgg ttaagaagat gaaagaggca ataaaagttc 20101 acagggaaaa aacaatggat tccttttatt cttccctgtt ttcttcgaag gagcaggaaa 20161 gcaaggaata tgtggcattg ttcaaagtcg atatcgatac aaatacagct tcatttaagg 20221 agcttgaggc cgggcttgac ttccttttgg ctgctgatat gaggatgcag ctggaaagca 20281 taaaagcgga tacgcttatc attcatggtg aaatggacag catatgtccc aaagaagcgg 20341 ctttgtatat ggcacaaagg atgaaaaaca aagcacaagt ctttatcatg gaaggcatag 20401 gtcacgtccc cttttattct ctaccggagg aatttacaag attgattttg gaatttatga 20461 agcaaggtga taaaagtgat agataaaaag attcttcaaa tgcactttag ccgtaatgct 20521 aaaaactatg acgcatacgc taaagttcaa aagaaaatgg caaatactct gctggatatg 20581 ctggacttgg atagtaagaa cagattggat attctggatg tgggctgtgg cacaggatat 20641 cttacgaagc ttttgcttga ccgttggccg gatgcccgaa taacggcaat agatattgct 20701 ccgggaatga ttgaatatgc acgggacaga tttaacgaga gcaatgtgga gtttgcctgc 20761 cttgatattg aagaagcgga acttaaccaa aaatatgacc ttgttatttc aaatgccact 20821 tttcaatggt ttaacgactt gggaggcact gtcaataagc ttgttcaaag ccttaaaagt 20881 gacggagtac ttgccttttc cacttttggg catatgactt ttagcgaact tcatttttct 20941 tatgaaactg cccgcagaaa actaaaaata gacgaagaat ttccgccagg ccaaaaattc 21001 tgtaacgcaa aagagatact taagatttgc tgtgagacat ttgaagggct tgagggcttt 21061 gaatttgata ccgtcaaaaa agaaagtctc gaatatgagt atttttacac ggtaagagaa 21121 tttttagatt ccgtgaaaaa aatcggagcg aacaacagta acaaacaaag aaaggtaaac 21181 accgctctta cgaaagaaat gatacggatt tacgaggaga tgtttaaagt aaatggattg 21241 gtgagggcaa cctatcattg catcttcata acatcaagaa agaaacttgc ggcaaataca 21301 agaaggctgg tgaacgcagt tgtatgactt gcaaagaaaa gacttggaat atatttggca 21361 tccctgctct cagatgaagg attatgaaga ttttcctccg attgttattg agaggggaga 21421 ggggccgtac atctttgatg ttctgggcaa tcggtatatt gatgcggtat cttcctggtg 21481 ggtcaatctt ttcggacact gcaacaaaag aatcaatgat gcagtcaaaa ggcagcttga 21541 cagccttgaa catgtgatat ttgcaaattt ttcccacagg cctgccattg agttggccgg 21601 gaaaatcgtt gaaattaccc cgcaagggct aaacaaggtg ttttttgccg ataatggttc 21661 ttcggccgtt gaaattgcac taaagcttag ttttcaatat cacttgcaaa ccggaagctg 21721 tgaaaagaaa aaatttgttg ccctgtccga tgcctatcac ggggaaacgc ttggagcttt 21781 ggcagtaggt gggctggacc tgtacagtaa aatctacaag cctttgatga tgagcacgat 21841 aaaagtgcca ggacctgact gttaccgatg caaatatgga aaggagcgca cctgttgtga 21901 agctgaatgt ttttgtgaaa tggaaaagat attggaagaa aaacacaggg aaatttgcgg 21961 agtaataatt gagccgatgg ttcaagcggc ggcaggaatg aaaatatatt ctgcaaagta 22021 tcttattaag cttagggagg cgtgctctta ttacaacatc cacttgattg ccgatgaaat 22081 cgcagtggga tttggaagaa cgggaaaaat gtttgcctgt gagcacggcg gtataagtcc 22141 cgatatcatg tgcctgtcga aagggcttac cgcaggctat atgcctcttt cgctggttgt 22201 taccactcag aagatttacg atgcttttta cagcgattat attgagctta aggcgtttct 22261 gcacagccac agctataccg gaaatcccct ggcttgtgcc gcagccttgg aatcgctgaa 22321 aatatttgag gatgacgatg ttttaaacaa gaacagggaa aaatccgggt tgttcagaaa 22381 gctggcagaa tcccttgccc atagccatcc ctatgtgggc gaatttcggc agataggtat 22441 gattgccgca ctggagttgg ttgaaaacaa ggcccagaag aaagagtttg attggaaaaa 22501 acgtgtggga tacaatattt ataagctggc cttaaaaaaa ggtttgctgc ttcggccttt 22561 ggggaatgtt ttatatttta tgccgccgta tgttgttggg caaaaagaca ttgaggacat 22621 tgtaagcgga gctttttatg cgataaatga gtattttggg cttgatttta agtataaaag 22681 gatgttccca taaaggttat aacctgatat aattatttga aattatattt tgacaggggt 22741 ataccgatgc aaaatgaagt atttgattaa gttttattaa aaaagagata gaaacttcaa 22801 aaagcgccgt agaaattttt aagctgtttt atgatgcaca ctacagcatt ttttagacag 22861 ttcgttaaac aataatggat tgggtgaaaa ttccataatt gtgtttgacc cttttttggt 22921 gtttgtatca aagggggaca ggataaaact cataaaaggt tcccacgtag aagagtatac 22981 tgaagacgtt ctattgtttt taaaaaagct acttggtaga tataaaaaat acatatgtgt 23041 ctgagttacc gtttttcagt ggggcggtag agtatttttc ctacgatttg tgccaccgga 23101 ttgaaacgtt taaagaacac ggaaaagagg atatgaacat acccgacatg atttttgggt 23161 tttataacaa tgcaattatt atagaccata aatgcaataa agtatatgct gcagtgtctt 23221 caataggctt tgaacgcaga gaggatatta atcaagtttt ggagagaaaa ataaacgaga 23281 taataaagaa agtttgtgag ggcagcgtaa agagtacaac cggaaaaaaa gcagcggaag 23341 gtcaaagtta tgttgcttcc aattttactt tcgaagagta ttgcagcata attggaaagg 23401 tcaaagagta tatcaaaaac ggcgacatat atcaggctaa tttgtcccag tagtttagaa 23461 cgaatattgg aggttctgat ccttttgata tatatgttga tttgcgaaag ataaatcccg 23521 cgcctttttc ggcttacatg aattttgagg aaataaaggt tttaagcagt tccccggaaa 23581 ggtttctgaa aataaacgga aagactattg aaacccgccc gataaaaggt acaagaccca 23641 gaggcaaaaa cgaagaagaa gcaacaagct taaacatgac cttttaaaca gcgaaaaaga 23701 cagggcggaa catataatga ttgtcgactt tgagcgcaac gatttgggca gggtgtgcaa 23761 aataggaagt gttcacgttg agaaattgct tcaaatagaa gagtattcta cggtccttca 23821 tcttgtttcg acggtaaaag gggaattatt ggatgacgtt gatataatag actgtattaa 23881 agcaacgttt cccgaaggtt cgataaccgg agcgcccaag attcggtcaa tggagacaat 23941 agatgaactt aagcctgtga aaagaaatat atacaccggt tcaataggct atatggattt 24001 taacggcaat tgagacttga atatggccat aaggactatt gtcataaaag gtaatgaagc 24061 ttttttcaat gtaggcggag gtatagtgtg ggattccgtc cccgaggatg aatatcggga 24121 aactcttgac aagggaaagg ctttgctcaa ggtattgaca gggaggtagc aggtagtatg 24181 gattgttttc atggtatcaa atctgaagat tttggggttt tatatggttt tagtttgttt 24241 gagacgtttc ttgtaaatga atacggaagg gtattcctgc ttgaaaagca tattgacagg 24301 ttgtttaatt aattcaatgg cgcatttcgg gtttgagact ggttttacaa aagacaattt 24361 gatgtccatg gtaatggatt atataaaaaa gtatgacttg aaaaacgtta tattaagact 24421 tactgtcacc tacggcaata agaataaagg tatagaacct gccgtctttt tttcaacgag 24481 ggaaaacacg tataaaaaag ctatttatga aaaaggcttt aaacttatgg tatccgggct 24541 tgtgaaaaat gccgattccc ctgtcatagc ccataaaacg ggaaactatc ttgagaatta 24601 tatggaaggc caaagaacgt taaagaatgg gtttgatgat gtcatttttt aaatgcaaaa 24661 tcggaaataa cggagacatc aaagagcaat atattctttg atttgtcacc aattccgtta 24721 taggtattgt gcctgtaaat agtataggaa ataaaggggg tggaagcgaa attcccggaa 24781 aagtaaccgg tttatttatg agagaacttg aaaatgtcat gtagatggat tgatttcacc 24841 gcccaaaacc ccatatatta gaatagatct caagcatcaa ttcttctaca cgcttataat 24901 cgggtttatc gggaagagaa gtgttttctg ctgccaatct gaatctgcgt tcatactcat 24961 caaccatttc aaacaactcg ttgtaagagt attttccttt acgtatgtca agcaaaaact 25021 cccgttcttt ttctctataa gtgttaattc ctttaccttc gaggacttcc gttcctgtaa 25081 tcaacagccg tattaagtgc atggcatgtt tgttaaggtg aggttcatct tttttgttgt 25141 tcctgtgatt tagtttggag taatttttca caacgttgct catatctgaa tagatatttt 25201 taaggtctct tagggggtag tgcttaaggt ttatatcaat aaatatttca gtttccattt 25261 caggtttgtc tgattgatca atataaaggt taatttcttc atttgtaaaa cttttgtaag 25321 ttctttttaa gtgctccatt tggcctaaaa tactctcaag aatatgctgc tccttttctg 25381 cttgagggta gctgtctctt gcaagagcgt ttttaagcct tcttagctgg gctgttgcat 25441 aatttccgaa actgtgtatt gcctttttgg acaagaataa atcagcatta tcccttaaaa 25501 gctgtccttc ctttgttatg acaagaagat gctcatcttt tgtgcctaaa atttcgagga 25561 catttgggtt gctatccaga cacaaactaa taaacttttt gagcccgtaa atcacagtgt 25621 ctgttattct gtcttcgaac tgttcaaagc ttgacaatcc taaaaggtct tgttttgttt 25681 ctattgtgac gcctctgata tcgatatcgc tggtatcaac atttgttcca taggcaatgg 25741 acccggctgt tgtaagaatt aatatgtttt ctccgaggcg ggggtctgtt cttagaaatt 25801 catatttatc gctatttaac ctgtctttta tgtcttgtat acccatactc ttactccttt 25861 tattttacgg aatatttttt attcatttcc atacttcttt aagggtctct ctaaataaag 25921 tatttaaagg ttcataatcg gacgtctctt ctttttttag agctttgaga tatttctcat 25981 aatggctgtt ttactaaaat aatactatag ataaaaagcc aagtccagta atatagtaag 26041 tgacataacg ggaatgataa ctgtgacttg aatattcata caataaaaat cttaaatatt 26101 tttattcttt actttttata ttttttactt ttcgcggaaa tacttaaatg ataaaatatt 26161 tgtaaacaat aatattaaga ttggtttggt catgatttgg acaaggtaag tacttttctt 26221 aaaattactt tgatatatgc caatctaaag tttatattcc ggatttacag gaggtattga 26281 ctatggcatt tgattttaag aaagaataca aagaacttta catgccaaaa accgaacctg 26341 aaataatcga tgttccacag atgaatttta tagcggtacg cgggaaaggc gacccgaatg 26401 aagaggatgg tgcttataaa caggccgtca atattcttta tgcaattgca tacacaatta 26461 agatgagcaa taaaagcgga cgcaaaatag acggtttttt tgaatatgtt gtgccgcctc 26521 tggaaggatt ctggtggcag gaaggcgtag agggagtgga ctacagtcaa aaggataagt 26581 ttaattggat ttcggtgata cgcctgcctg attttgtttc aaaagaagat tttgagtggg 26641 cgaaagaaga agctgcccgc aaaaagaaaa tagactgctc tttagcggag tttttaacca 26701 ttaaagaagg actttgtgta caagctctgc atttaggtcc ttttgatgat gagcccaaaa 26761 cagttgcaaa gatggatgaa tatttgataa aagaaggata tgaaaaggac ttttccaacg 26821 aacggctgca tcatgaaata tatttgtccg acccccgcaa ggttgaaccg gcaaaatata 26881 aaacggttat acgccatcct gtgaagaaaa aagaaaagta cctttagaaa ttttggtata 26941 aattttaaag gtacttttct gtattttaga tatttaattg gctaacaaat cacttacttt 27001 tgtgtaacgg gaaattttgt aattgctttc attataaatc tctttaatat tgtcaaatcg 27061 gttgaattga ttgaaccgtc accgtttaaa tctgctgctt tttcagggac attcattctt 27121 tcaagaactt ttaaaatata tttcttcagt atcatataat cggttgagtt tatatttccg 27181 tcattgttca aatcgccgta gttgatatcc ggtatttcac catcatattc ataaaggttt 27241 tccggaagtt catccaaggt gattacatat tcactgttgt agatagtgtt caaaagtacg 27301 gggtcgttgt atctgggact cataaggaaa tcaccgtacc atgggcagaa atacagccac 27361 caggcttcct cattaactat attttgaata tcgggaataa cgtcattttc agtcaatgca 27421 accatctttg tgtcgtttgt gtaatttaca agtgtaagga ataatgatga tgcggcgctt 27481 gtgccccagg tattgggtga accttcatat ttgtcgtatc cgataatgtc cacatactca 27541 tcgccgggat accattcata agaattggca tatgtataaa ggtttacttc ccatatcaaa 27601 ttatgaatgc cgtatttttc ggtaagagtt ttataaagaa gtttccagag ttccttgtaa 27661 acttcagcac ctgcagaacc ccaccagaac catgcgccgg agccgtcggt gttgttgtag 27721 ccttcagctt catggaacgg acggaaaagt ataggaatgt tttgctcctg aagaattaaa 27781 agctgctctg caaggtcttc aattgccatc atcaggtaag catgttcttt tgttgtttca 27841 tcaaggcagt ttgcggtatt aaagctgctt gtcggtttgt atgtacagtt tgtccaatcc 27901 accggctcac cgagtttata acttgcaaaa tccctgggaa tatttatatg ccagcatgct 27961 gttgcaatac cgccgcgatt ttttacccag tcgattatac gtgccgttgt accgtcttcc 28021 catccgtaca gaggattgta gttcatgaaa tcaaagcctc tgattgcagg atatttgcct 28081 gtcttctcat aaatataatc gaattcaagt tcataattgc cgtcgtttcc gtttccgtaa 28141 atctcctgct ggccggaaat aacatatttt ccgtacacgc tggtaaggta tttcataaga 28201 gctcttgttt caggagttgc atttggatca cacggagtcg ggtcaattat atgatcgggc 28261 atgtcagcat ggtcaaagta tattttgtcg tacagtataa agccccagct tccggaggaa 28321 cctatctcaa tagttgcttt accggcctca agatagaaga atccgaaact gtaatcaatc 28381 cattggcctt tgtttggaat aaaatagttg ctgtgtgatt ttccgttgat gcttataacc 28441 tgcattctgg tctcatcttc tttgccaaga tacatccagc atcttgtgga aagctcatac 28501 ataccgtttt caggaatggt gacttccaat gttatcgttc ccgagttggc cacccatacg 28561 aatccgtcgc cggaatatcc gggatattga gttccgtata cattggtggt aacaacggca 28621 ccgttgccaa gagtgcaatc ttctgcttca acgtccacag gaagggaata tgcagcataa 28681 gttgaaacgg gcatgactaa aagaacggaa aacaccattg caaatgttac cagcagactc 28741 agtgtcctgc caaaaatttt ccccataaaa atacactcct taaataataa aaaatttatt 28801 aaaaataaaa ataactatct cattaataat aattagtaaa agtatttatt ttttgtgtac 28861 atgaaaaaac tacaattatt gggaagttgg cgcgaaatac ttttggaaag tatactgctt 28921 aaaagtgtat tgtttgttgt tatgtaaaga cgtgcaactc cttatactaa ttaaatttta 28981 gcacattcat cgcataatat caattattca aatcatgacc gggtttatgc cggcaggcgc 29041 gttacattta caaaaatcat tttactgtag tataataagc ttgtttctat tgatactaaa 29101 tgaataaaaa acgaccggga aaagagaaaa gaatatgaag aagttggtag ttggaatatt 29161 ggcgcatgtt gacgcaggaa agacaacatt atcagaggcc ctgctgtatg tgagcggaaa 29221 aataagaaaa ttgggaagag ttgataacaa agatgcttac ctggacacct atgaactcga 29281 aagggccagg ggcatcacta ttttttccaa gcaggccgtt tttgaagtgg gggacacccg 29341 gattaccttg ctggatactc cgggccatgt ggatttttcg gctgagatgg agagaaccct 29401 tcaagtgctg gattatgcca ttttagttgt cagcggtgct gatggagtgc agggacatac 29461 caaaaccctg tggcatcttc ttgaaatcta caagatacct gtgtttatat ttgtcaataa 29521 aatggaccag aacgggacaa acaaggataa agtaattaat gaaataaaaa agcagttgga 29581 tgacagatgc atagaattta gtgaagaaaa tccagaagag ttttttgacc ggctggcaat 29641 gtgtgatgaa atgataatgg agacgtatct tgagaaagga cgggttgaaa cctcgcagat 29701 tagtgctgcc gtaaaagaac gaaaggtatt tccgtgtttt ttcggttcgg cattgaaatt 29761 ggaaggtgtc gaggaattta ttcacggtct tatgaaatat actgtagtcc caagttaccc 29821 taatgaattt ggtgccaaga tatttaaaat aacaagggac gagcagggaa accgtcttac 29881 ccacatgaag ctgaccggcg ggaaactcaa ggtaagagat gttttgacaa acggcgtgtg 29941 ggaagaaaaa gtgaatcaaa tccgcatcta ctccggagaa aaatttgaag cggtaagcga 30001 ggtggatgcg ggcaccgtgt ttgcagtgac cggccttacc caatccagac cgggagaagg 30061 tcttggaatt gagaaatctt caggtgcgcc gctgttggag cctgtgctgc agtatcaaat 30121 catacttccg gaaggttgtg acccaagagc gatgctgccc aagcttaggc agattgaaga 30181 tgaggaaccg gagcttaaca ttgtctggga tgaacagttg caggaaatcc gggtccgggt 30241 catgggagag gtgcagattg aaattttgca aagccttata aaaagccgtt ttggagttga 30301 tgtgactttt gacgacggaa gtatagtata taaagagact attgccaata ctgttgaagg 30361 agtggggcat tttgagccgc tcagacacta tgcggaagtt cacttgctat tggagcccgg 30421 agagagggga agcggcctta agtttgatgt aaactgcagt gaggatgttt tggctaaaag 30481 ttggcagaga ctggttttaa cccaccttga agaaaaagtt cataaagggg ttttgacggg 30541 ttcggcgatt acggatatga aaattacttt ggtatccgga agggcgcaca acaagcatac 30601 tcagggcggc gacttcaggg aggctactta ccgtgcggtg cgtcagggtt tgaaagaagc 30661 ggaatccata ctgcttgagc cctattatga ctttcagctg gaagtgccgg aaaaaatggt 30721 gggaagagcc atgatggata ttgagaaaat gcacggcaca tgtgagatat cccagataaa 30781 cggcgatatg gcagttttgg ttggcagcgc tcccgttgtc accatgagga attatcagaa 30841 agaggttgtg gcatatacca aaggtcttgg cagactgttt tgcagtttta aagggtatga 30901 gccgtgccat aatgcgcagg aagttataga gcgcatagga tatgattcgg aaagggatgt 30961 ggaaaatccc acaggttccg tattttgcgc caatggtgtc agttttttgg taagttggga 31021 tgaagtaaag aattacatgc atgtggagag ctattttcag aaaaaagagg atgaagaaaa 31081 tttaaaccaa aaccggcctg tgtattcggg agaaaaagcg ataagcctgg aagaaattga 31141 tcaaattatg aataaaacct tttatgcaaa ccagggaagg aaatctgcat ggaagaggca 31201 aaaggcgtcg gaagaaaggt attacaaacc tgcagccggt gcaaaaaggc atgaggcaaa 31261 agaagagtat cttttggtgg acggatataa catcatctat gcatggccgg agttaaaaaa 31321 gcttgccgat gagaatttgg acggcgcaag aatgaaattg cttgatatgc tgagcaatta 31381 ccaatggatt cgaagatgtc atgtcattgt cgtatttgat gcttaccgtg ttgagggaca 31441 taaagaagag ataatagatt attataatat ccatgtggtt tatacgagag aggctcaaac 31501 agcggatcag tatattgaga aatttgccta tgaaaacagt aaaaactatg atatcactgt 31561 tgccacatcc gacggattgc agcagataat tgtaagggga gaaggatgcg cactgctgtc 31621 cgcaagggag cttaaggctg agcttgaagc tgccaatgaa agaataaaac aggaatacca 31681 taaaatgaat aaaatagacc gcaattattt gggcgatgct ttgtctacga aagcaaaacg 31741 gcatatggaa gatttgattg aagaagaaaa taagaataac ggaattaaaa aagataaata 31801 attaaggaat gtaaaatttt gcatttataa tatgatgaat ttcatattgt ggacaattta 31861 tggtgattaa aaatttatgg tgattaaatt tatagtgatt aattatagaa attcaaatta 31921 caataattca aaatggtttt tgttataatt taaaatacct tcttttctaa gcttgctaag 31981 ttccgccgac attgcgctgc ggtcaaccga cagataatct gcaagttcct gacggttgaa 32041 cggaatggaa aaacgattgc tgcctgcctt ttgtgcctca ctggaaagat aggccaaaag 32101 cttttcccga gtggtacgct ttgaaataaa ctcaattttt tgtgttaaag atatattttt 32161 catcgcagta atatgcagca tattttgaat caggcgactg tgaaaagcgc aggcagtagc 32221 acatggcttt gcgagtttac ggcagtcgat aaaaagaaga tcgctttcgg tggttgtaat 32281 tacactgacc ggaagagctt ctatttgagc acaggcaaaa gattcggcaa acagattgcc 32341 cacacctatc ctggccagga tacttctgtt accgtaataa tcttcctgta caatctgaac 32401 ctctcctgac aaaactatgc caaaagaatt aatggcctca ccgctcataa aaacaatctc 32461 gttttttttg taatgctcaa gcttgcttga caaacaggcc aaaagcgaaa gcaaatccga 32521 ttcgtcaatt cctttaaaca aattaacttt ttttaaaaca tccaaatatt ttttcatagc 32581 aacctctttt tgttgtattt ccaacagact tatcagtttc agtatagtaa acttaaaaca 32641 taaagtcaat gaaacaaata taaaaagttc aaaaaacagg aggttagtaa aatgattcgt 32701 agagtaataa agatagatga agataaatgc ataggatgtg aattgtgtgt aaatgcctgc 32761 cacgagggag ccatagggat ggtgaacgga aaggcgaagc ttttgcggga tgattactgc 32821 gacggcctgg gtgactgcct tccggtttgc cccacaggag ccatcacctt tgaagagcgt 32881 gaagcggcag agtataacga agcagccgtt caggaaaaca agctgaaaaa gcagaaagaa 32941 gatttgcctt gcggatgtcc cggtacgcag atgagaacta tcaacaggga aaatgaacac 33001 aaagccgagc ctgcggagac ttacgagaac atgagccggc tttcccagtg gcctgttcaa 33061 atcaaacttg taccggtcaa tgcgccgtat tttgaaaatg caaatctttt gattgcagcg 33121 gattgcaccg catatgccta tggaaatttc cataataaat ttatgaaaaa cagaataact 33181 ttgattggtt gtccgaagct ggacgatgtg gactacaccg aaaagcttac ggcaattatc 33241 aaaaacaaca atatcaaaag cgtcacagtt gtgcgtatgg aggttccatg ctgcggagga 33301 attgaaaacg cggtaaaaag agctctttta aacagtggaa aactcatacc ctggcaggtt 33361 gtgacaattt caaccaacgg cgaaatcctt gaataaacgg ttttataaac cttatttata 33421 gacctaaata aaaaagcata aaaggaggaa aagcaatgag catgttttgt tatcaatgtc 33481 aggaaactgc aggaggaaaa ggctgcacag tacgcggggt gtgcggcaag aatgaggaag 33541 ttgcaaagct ccaggatctt ttgctttaca ccgttaaagg catctcatat atcgttacca 33601 agggaaatat tgatgccgca aaacttggaa atacaaacca tgaagtatta agcagcctgt 33661 ttatgacaat taccaatgtg aattttgacg atggttcaat tgaaaaacag ataagaaaaa 33721 tgcttgccgt aagggacgaa atgaaaaagt ccgtacaggc ggaaggcctt catgatgcgg 33781 cggtattttc tgtggattca agagaatcca tgttaaaaaa agccgattct gtgggggtac 33841 tctccactca aaatgaagat atacgttcat taagagaaat gattacctat ggtgtcaagg 33901 gtatggcagc ctatgccgag catgcgaaaa atataggaaa agaagacaag gaaatctatt 33961 cctttatata tgaggctttg gcggcaactt tggacgactc tctttcagtt gacgaccttt 34021 ttgcgctgac actcaaaacc ggtgaatacg gtgtaaaggt tatggcgctt ctggacgagg 34081 ccaatacatc aaggttcgga aatccggaaa ttaccgaagt taatatcgga gtcagaaaaa 34141 atccggccat ccttgtttcc gggcatgacc ttaccgatct tgaacagctt ttggagcaga 34201 caaaaggaac aggcgtggat gtatacaccc atggcgaaat gcttccggcc cactactatc 34261 cggctttcaa aaagtatgac aatttcgtgg gcaactacgg aaacgcatgg tggaagcagg 34321 tggaagaatt tgagtccttc cacgggccta ttttgtttac tacaaactgt attgttccgc 34381 caaggagcga agaggtcaga agaaggatat ttacaacagg ttcggcaggt ttccccggat 34441 gcaagcatat tgaagccgat gaaaacggca agaaggattt ttcggagata attgagcttg 34501 cgaaaacttt gcctgctcct gatgaaattg aaacgggaag cattgtcggc ggatttgccc 34561 acaatcaggt aatggccctt gccgacaagg tggttgaagc cgtcaaatcc ggagctgtta 34621 aaaagttctt tgttatggcc ggatgcgatg ggcgcatgaa gtccagaagt tattacaccg 34681 agtttgccca aaaccttcct aaagacactg tgattttaac ggcaggatgt gcaaaatatc 34741 gttataacaa gttgggactg ggcgatatag gcggtattcc gagggtgctt gatgccggac 34801 agtgcaacga ttcgtattct ttggcggtta ttgccctgaa acttaaagag gtatttggac 34861 tggacgatat caacaagctt ccgattgcct ttaatatagc gtggtatgag cagaaagccg 34921 taatagttct tttggctctc ttgtatctgg gcgtgaagaa tattcatctt ggacctacgc 34981 ttccgggatt cctttcaccc aatgtggcaa aggttctggt ggagaagttt ggcatcgcag 35041 gtattggcac tgttgaagat gatatcaaat tgtttatgtc ttaaaactta aaataaagca 35101 tggaataaca gacaaagggc caatcggcaa tgattggccc tttgtacagt atagtgcaat 35161 attttaaata aatttttaat taatgttaat gtatatcaat ctttttgcct ttcatttgtg 35221 ttgtttcaac tttaggaacc gtaacggtca aaataccgtc tttatattct gcctttgcct 35281 gttctgattt tacttcgaca gggaggggaa ttgttcttga aaagcttccg taatatcttt 35341 cagtcctgta tatatgttca tttttatatt ctgtatctct ttttgtttct cctgacaatc 35401 ttatggaatt ttcatcaaca tatacattta aatcttcttt ggatacaccc ggtatttcag 35461 cctttacaac aacatctttt tctgtttcga aaacatcaac tctgggagct gttgctctgc 35521 tgaaaaaacc aaaaggtgaa cgttcaaaga aggtttccat atctctgctg atgctgtcta 35581 cttctctgaa tggattccag gaaatcaggc tcatacatat cactccttgt ccttataaga 35641 gttttttcac aaataatatg atttccgtta attttttaat tatgcggttt gattgccttc 35701 atgccatatt gacatcatgt gaaaactgta ttagaattat taatgctttt taagtaagtc 35761 taagctttgc cactaggtaa agtttcagag gcgttctgaa acatcctgcc gacttttggg 35821 aaggatgtgg aagaacgcct tttcgatttt gatggaggat tttagatgga agaaagattc 35881 aagaatattg atagctttat gaaaatttta tgaaaattaa aaaaataaga aggaaatttg 35941 gaatttatat agaaaatatt aatcataaat ttaacataat atatttacat aatacaatta 36001 cgtttggcaa catctgcaaa ttgtttatag cacgggaagg agtgataaag cgccaataca 36061 atgctttgat atgcttttga taaaatcatt tctgagtatt aaaaatgtgt agaacgctga 36121 aagaattgca tttttgagaa tgattgaagc atatcataat tttcataacc tatcctattc 36181 ttatataaaa aactcttggc aagatcataa attcattcgc gctgacaaat taatagcagt 36241 ttaattcttt tgctgggaga tgtttgtatc aactgaattt gtgactaaaa tccggcgaga 36301 atggctctgt ttgttgaagg agctatcttt ttattctttt tttgagacat taggtttaca 36361 ataatgtaaa caagtcctaa tagagataat tttatgtaaa ttaaaactaa cagtggcgaa 36421 gtcgctgcaa ataatagcca tacataccgt aaaggaggtg agagagaatg ccggagatac 36481 tgacaaacga attagaactg cagcaagagg aaacccaaag ggacaaatat cttaccttca 36541 ttgttgaaaa ggagtactat gggattgaaa tcaggtatgt tacggagatc atagggattc 36601 agtctattac cgaagtaccc gatcttccta aatatattcg cgggataata aatcttcggg 36661 ggaaaataat tcctgtgatg gatgtcaggc tgagattcgg caaggaattc agagagtatg 36721 acgacagaac ctgcataatt gttattgacg ttgacaatat gtttgttgga ctgattgtag 36781 acagcgtgtg tgaggtaatg tccattccgg acggggagat tgttgtccca cccgattttg 36841 accaaagtga aaacaagttt atcaagggca taggcaaaac cggaaatgaa gtcaggctga 36901 tactggactg cgaaaaactt ctcaaagatt gaataagtgc aatactcaag gaatgaaata 36961 taaaaaatca aggaggatcg ttatatgctc agatggtatt ataatttaaa aatatcagca 37021 aaattaatta ttggttttct cctgcttgca ttggttgcag gagttgtcgg agttgttgcc 37081 ctttcaaaca tcaataatat gagccaggcg gatgccgaac tgtatgaaaa aaacacattg 37141 ggtatcaact atgctgccgg tgcttccttg aggtttcaga gaatgagata taatactgca 37201 aaacttctgg tatatgatgc agaacaggta agtaaaggca tagaaaaaat tcaagaacat 37261 gttgaaaata ctgaaaaata tttaagtttg tatgaaagta cagttataaa cgaaaccgac 37321 cgcattcagc ttcaagaatt aaagacgttg tgggaaaagt ataaatcttt ggttgacaaa 37381 gaagttgaac ttgttaaatc ggggaaaacc gaagaagcaa gacagttgct tctttcagat 37441 attgatgata ttggagatac tctgagagac tattttgagg cttttgtgga atataatact 37501 actgcagcga aggaaaaagt ggatgaaaat aagcaagttg cgtcaactgc ttcaactgta 37561 atgatagttg tgatatttgt aggcatatta atagcaattg ctttgggagt gtttatatcc 37621 aggattatca gcaaacctat cggccagatg gtggaagctg ccgacagact tgcccttgga 37681 gacgtggaag tggatgtcaa ggctgaaacc agggatgaat taggaaaatt ggccgaatct 37741 ttcaggagaa tgatagaaaa tatccgtgaa caggcgtatg tagtagaaaa aattgctgag 37801 ggagacatga ctgtcgatgt aagagtcaaa tccgacaaag acctgctggg taaaaaactt 37861 aaggaaatgg ttgagataaa taatgaagtg ctttcaaata tcaatgaagt tgctgcacag 37921 gtggcagcag gagcaaaaca ggtatccgac tcaagcatgc agctttcgca aggagcaact 37981 gagcaggcaa gctcgataga agagctgaca gcttcccttg aacaggtggc gaaccagaca 38041 cagattagtg ccaagaatgc gaatcaggcc aatgaactgg ctgaaattgc gaagaacaat 38101 gcggagcaag gaaacaagca aatggctgaa atgctcaatg ccatggaaga aatcaataat 38161 tcttcatcaa acatctccaa aattatcaaa gtaatagacg aaattgcatt ccagaccaat 38221 attcttgcgt t //