LOCUS NZ_ACVX01000036 35347 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00022, whole genome shotgun sequence. ACCESSION NZ_ACVX01000036 NZ_ACVX00000000 VERSION NZ_ACVX01000036.1 GI:256005659 DBLINK Project: 55637 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. REFERENCE 1 (bases 1 to 35347) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Hemme,C.L. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The draft genome of Clostridium thermocellum DSM 2360 JOURNAL Unpublished REFERENCE 2 (bases 1 to 35347) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Hemme,C.L. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (17-JUL-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000036. URL -- http://www.jgi.doe.gov JGI Project ID: 4085028 Source DNA and Bacteria available from Christopher L. Hemme (hemmecl@ou.edu) Contacts: Christopher L. Hemme (hemmecl@ou.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Annotation by JGI-ORNL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Clostridium thermocellum DSM 2360 Culture Collection ID :: DSM 2360 GOLD Stamp ID :: Gi02594 Isolation Site :: contaminant of Clostridium thermocellum strain LQ8 Isolation Country :: USA Oxygen Requirement :: Anaerobe Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Sporulating Temperature Range :: Thermophile Temperature Optimum :: 60 C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Phenotypes :: Cellulose degrader, Ethanogenic, Ethanol production Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Location/Qualifiers source 1..35347 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene 145..1674 /locus_tag="ClothDRAFT_2478" CDS 145..1674 /locus_tag="ClothDRAFT_2478" /inference="protein motif:PFAM:PF03747" /note="PFAM: ADP-ribosylation/Crystallin J1; KEGG: yen:YE2628 hypothetical protein" /codon_start=1 /transl_table=11 /product="ADP-ribosylation/Crystallin J1" /protein_id="ZP_05430617.1" /db_xref="GI:256005660" /db_xref="InterPro:IPR005502" /translation="MAIIPSVTEKCTGAMLASAIGDALGWPNELGAKNSIKNTKVNDQ FIEWTRRSGGRYWPYYEKILPGEYSDDTQMILAVARSVITGNWESTLAFKEIPFWLKY ERGGGSALKEAAKLLKKGIVPWEARENNKYFMAGGNGAAMRILPHVIHKQNIDLLILD VIKDCLYTHGHPRAILGASCYAYALYYLRNKKDVLEYGELIRAVIDGLKYWTTLNALP AQWLVLAEKHSGFDYLQIWFNTISNMAQKLEYIFYSLDKGLLINDDDVLKYLGCFDET SGAGDVAILSALYLASKYANNPMLGIKIAANLHNADTDTIASITGGLLGMLCGTEWIP FEWRLVQDYDCLVNITQILLADNMIETSRHISNKNKVQHNDWKDSPIGKMRILTRTVV PAGKSRSVIIDKFETTLGQTLYIKEFKDNRIIDSSQKEQENKTQTIINKKDIHHSSFL IDSNNIQELLDNPLVGRISFKKVLKIIDLLINSDLSCSEVANKLNICEEVIRLIKMQL R" misc_feature 181..1155 /locus_tag="ClothDRAFT_2478" /note="ADP-ribosylglycohydrolase; Region: ADP_ribosyl_GH; cl00614" /db_xref="CDD:153890" gene 1713..3803 /locus_tag="ClothDRAFT_2479" CDS 1713..3803 /locus_tag="ClothDRAFT_2479" /inference="protein motif:PFAM:PF02463" /note="PFAM: SMC domain protein; KEGG: efe:EFER_2804 hypothetical protein" /codon_start=1 /transl_table=11 /product="SMC domain protein" /protein_id="ZP_05430618.1" /db_xref="GI:256005661" /db_xref="InterPro:IPR003395" /translation="MNCMYISKIQIKNFRNFKQFEAHFNNGINVIIGHNNSGKSNLIK ALALIFDSNTKKHLEIDDFNKYISLEDLKNEPPKISIAITITQQEDENLMSDDLVTIS NWLTKLEEPYEALLTYEFMLPVKEHERYKKKVNNLEKEEEIWEVINRDFLRLYTYKIW GGDPINKTVADSESLQKFDFQFLNAIRDVERDMFTGRNTLLKSVLDFFIDYDIKSDKE KTEDEKLSEISEIKRKFSIDANNLIKSLQKRMEKGKKEILSYAYNIGASFDNSYPNFD GNISDVQLYSALSLIVEYKTGIKIPVSHNGLGYNNLIFMALLLAKMQINSDGTYLGSN AKVFPILVIEEPEAHLHPSMQYQLLKFLNTNIKQNKVRQIFVTTHSTHIAASVSLDQM LCLYKYKNDTQIAYPGKVFPDEKSKKYVQRFLDATKSDMLFSEKVILVEGIAEQLLIP IFARYLECPLEEKHATVINVGGRYFNHFLYLFDENNPFAIKRKVACLTDRDPERKEKS QSRYKKCYPFEFNLQPDTYDYRYNTLLDKYEVRENHKRICQNIASFKQDKNSSTFEYD LILSNPSLDILVTESLKNSEEIKSLMQAYKNGEPLDEFYKLLRNNPENKRIIESIEKA NSKEWSEDEKKKAIIASRYLNSVEKGENALELAYVLKENLDKKGTEDFQDFNVPTYII EAIKWVCQDDDNKF" misc_feature 1722..3236 /locus_tag="ClothDRAFT_2479" /note="Predicted ATP-dependent endonuclease of the OLD family [DNA replication, recombination, and repair]; Region: COG3593" /db_xref="CDD:33393" misc_feature <2730..2978 /locus_tag="ClothDRAFT_2479" /note="P-loop containing Nucleoside Triphosphate Hydrolases; Region: P-loop NTPase; cl09099" /db_xref="CDD:158411" misc_feature 3006..>3236 /locus_tag="ClothDRAFT_2479" /note="TOPRIM_OLD: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in bacterial and archaeal nucleases of the OLD (overcome lysogenization defect) family. The bacteriophage P2 OLD protein, which has DNase as well as...; Region: TOPRIM_OLD; cd01026" /db_xref="CDD:173776" misc_feature order(3033..3038,3045..3047,3207..3209,3213..3215) /locus_tag="ClothDRAFT_2479" /note="putative active site; other site" /db_xref="CDD:173776" misc_feature order(3033..3035,3207..3209) /locus_tag="ClothDRAFT_2479" /note="putative metal-binding site; other site" /db_xref="CDD:173776" gene 3787..5526 /locus_tag="ClothDRAFT_2480" CDS 3787..5526 /locus_tag="ClothDRAFT_2480" /inference="protein motif:PFAM:PF00580" /note="PFAM: UvrD/REP helicase; KEGG: abb:ABBFA_002186 UvrD/REP helicase family protein" /codon_start=1 /transl_table=11 /product="UvrD/REP helicase" /protein_id="ZP_05430619.1" /db_xref="GI:256005662" /db_xref="InterPro:IPR000212" /db_xref="InterPro:IPR014016" /translation="MIINSDTRIPIENHFRVSAGPGAGKTHWLVNHIKNVLQNSTRLS NARKIACITYTNIAVETILKRLGTAADQVEVSTIHSFLYRHVVKPYCSFIASDVGLNL EKFNGHNDTIVSNSRIKHWLENLPCKDQLKKPCECNQLLYSKVHRKALIKWLETLYYS FDNDKLYITGDSKKAYYGKLHLTNQCLKILESELFQYKKLYWKDGIVDHNDILYFSYK LIEKYPFIIQILRAKFPYFFIDEFQDTNPIQLSLIRKIGQKETIVGVIGDKAQSIYSF QGASMTQFEKFYLPNLIDYVMVDNRRSTNQIVDVLNLIRKDIQQNKFRKVEGDLPVIL VGDRNKGYEKAKELCNNSEICTLTRDNATANSMKKEIDENISDKNLFLELKILDSNSK RCNVVYSCIQAVELANNGNYKEAIKKLEYLFTERNDKHAVKKMALSCLCLLINNYNSF KDKTLMDFYSFVKDKIFTDLSKFRKGNIKTFYEKHTYQQLAAYVNIVDDISYFKTIHK AKGDEFNNVLLILKEKDLDFLLKPNLLKNEEHRIYYVAISRAKEKLFICVDNLNPQNI LKLNTLFNIVILN" misc_feature 3814..>4761 /locus_tag="ClothDRAFT_2480" /note="UvrD/REP helicase; Region: UvrD-helicase; cl14126" /db_xref="CDD:187234" misc_feature 3817..5454 /locus_tag="ClothDRAFT_2480" /note="Superfamily I DNA and RNA helicases [DNA replication, recombination, and repair]; Region: UvrD; COG0210" /db_xref="CDD:30559" gene 6111..6314 /locus_tag="ClothDRAFT_2481" CDS 6111..6314 /locus_tag="ClothDRAFT_2481" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430620.1" /db_xref="GI:256005663" /translation="MLDVEVEEKAGPKGKHNKDRTVYSYGTEKTSVVLDGRKVKIERP RLCVYKIRKLTLKDSNSFSRRTR" gene 6341..6613 /locus_tag="ClothDRAFT_2482" CDS 6341..6613 /locus_tag="ClothDRAFT_2482" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430621.1" /db_xref="GI:256005664" /translation="MGITTRNYNECQDTVPGIETFRAGKSNASRRFVQETSKRMKEFI SRGLDDIHIIIIATRSRIWRISCIGFTSSEEYRRKKVLGIKGAVRT" gene 6792..7142 /locus_tag="ClothDRAFT_2483" CDS 6792..7142 /locus_tag="ClothDRAFT_2483" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: nha:Nham_2785 transposase, mutator type" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430622.1" /db_xref="GI:256005665" /translation="MLDSERSKVIREMNETYTEFNYKETKKNLEGLASSLEHKYLVEA SSLDEGLDEILTLHRLKVPGLLRISFLTTNLIELARETARDIMGRVRGWSKGNQVLRW LSCVFLHARNSLSK" gene 7103..7231 /locus_tag="ClothDRAFT_2484" CDS 7103..7231 /locus_tag="ClothDRAFT_2484" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430623.1" /db_xref="GI:256005666" /translation="MCFFTREKQFKQVKGYKLSPVLISALFRGGGITTYFNNTLVG" gene 7503..7910 /locus_tag="ClothDRAFT_2485" CDS 7503..7910 /locus_tag="ClothDRAFT_2485" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430624.1" /db_xref="GI:256005667" /translation="MDNRKKLILGTVIFIGVILIIGFTLFSTLTLEPSVKVGYVGSNA GNTINASFKYFDGVESRKVKFNEGDVVTIKYAIGLEEGSLSLVVFDEKGDEIIKRSDN EGEVSFEVTETQKYKISLVAAKAKGNFSVKWDV" sig_peptide 7503..7586 /locus_tag="ClothDRAFT_2485" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.607) with cleavage site probability 0.295 at residue 28" gene 8218..8562 /locus_tag="ClothDRAFT_2486" CDS 8218..8562 /locus_tag="ClothDRAFT_2486" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: pat:Patl_2652 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430625.1" /db_xref="GI:256005668" /translation="MKLNGYELICPICKGKKFEKRNSLLNTRGMTFLNLDWLNEGAIN YICDTCGYILWFIDDGREYVEEYDEKEKIGIKGLHIDYETSKADEDECPVCFSKRDVN DKECSNCGYKFE" gene 8687..9127 /locus_tag="ClothDRAFT_2487" CDS 8687..9127 /locus_tag="ClothDRAFT_2487" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430626.1" /db_xref="GI:256005669" /translation="MLKHSSICLFLSSVFVILLFSECQTGNNWEVSQRSVIMMQSFSF IAKIFYIISITIFIMNLKNYIKIRNRLKFIMIIWFLLLAVPLIIVQLNGIPVDSLRIV GTEFFRQSYIVELVGMGTSLIFLVILLIVFRGRQAIDETTESKD" sig_peptide 8687..8764 /locus_tag="ClothDRAFT_2487" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.846) with cleavage site probability 0.492 at residue 26" gene 9235..10254 /locus_tag="ClothDRAFT_2488" CDS 9235..10254 /locus_tag="ClothDRAFT_2488" /inference="protein motif:PFAM:PF01370" /note="PFAM: NAD-dependent epimerase/dehydratase; 3-beta hydroxysteroid dehydrogenase/isomerase; Male sterility domain; polysaccharide biosynthesis protein CapD; dTDP-4-dehydrorhamnose reductase; short-chain dehydrogenase/reductase SDR; KEGG: sat:SYN_01112 UDP-N-acetylglucosamine 4-epimerase" /codon_start=1 /transl_table=11 /product="NAD-dependent epimerase/dehydratase" /protein_id="ZP_05430627.1" /db_xref="GI:256005670" /db_xref="InterPro:IPR001509" /db_xref="InterPro:IPR002198" /db_xref="InterPro:IPR002225" /db_xref="InterPro:IPR003869" /db_xref="InterPro:IPR005913" /db_xref="InterPro:IPR008089" /db_xref="InterPro:IPR013120" /translation="MEGVILVTGAAGFIGFHLVQRLLKEGCNVVGIDNLNEYYDVKLK KDRLKLLSENKNFVFRKVDIKNKKAVDRIFETYRPSYVINLAAQAGVRYSIENPYAYV DSNLVGFVNILEACRKYPVKHLIYASSSSVYGGNKVSPFSTRHNVDHPVSLYAATKKS NELLAHTYSHLFGIPTTGLRFFTVYGPWGRPDMAYFSFTKDILSGNPIKVFNYGKMER DFTYIDDVVEGIVKLIDRIPTPNENWDETKDDISTSFAPYKIYNIGNNNPVPLMNFIS VLESALGKVAKKVYLDLQPGDVLRTYADISDLERDINFKPSTSIEDGLRKFVQWYKEY YKAEI" misc_feature 9241..9639 /locus_tag="ClothDRAFT_2488" /note="Rossmann-fold NAD(P)(+)-binding proteins; Region: NADB_Rossmann; cl09931" /db_xref="CDD:186874" misc_feature 9247..10245 /locus_tag="ClothDRAFT_2488" /note="Nucleoside-diphosphate-sugar epimerases [Cell envelope biogenesis, outer membrane / Carbohydrate transport and metabolism]; Region: WcaG; COG0451" /db_xref="CDD:30800" gene 10402..12108 /locus_tag="ClothDRAFT_2489" CDS 10402..12108 /locus_tag="ClothDRAFT_2489" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430628.1" /db_xref="GI:256005671" /db_xref="InterPro:IPR007087" /translation="MIKRFIVFFSILLVLIIPTCVKVDASDIANLGFVETDVILQPDG KAIVSYTVRYNLVPGKTMLAFTMSGFDKLSPVFDTENAWVITDDNTSYPIDIVNLGNG YYDIINSGNKRLGGEYLTYKFRFVADMAAAGYLKKTTAEDGRRLVVFDWAPTQWEEAM EHYTVTVNYPLAFPKESGTREEVESFLLEHDFATEKWMNEKYLIDYRVQVLDGTPRVQ ILLHKENLAAYDKFEIKQYITEDIFNIDDPADIDFPPNEFGKGPFDRYEENMDRQPEN VNNGSEGRIILLTILIGLFVMIYIIVGRKHSSVMKARETLDQIQWARQDWDPPKIEIA SFRKDGTVATGLDAVEIALIIGIPYKRIISVIISKLVEQGFLEEISEEPLRVRRIEHD NASAVLNEYELMVYEAAKDGEIDETEIENIIQRLTDNVRQKTWDCDIEATRKYYADKM SELLKKENADPAQAADNSYTGDDYIYAWPYWYYFHSGLVYHRQRDRHERYERSVPVNP GRLSFNSALDPDTGAFACHSACHSACHDACHSACHSACHSACHSACHSACHSACVSGG AR" sig_peptide 10402..10479 /locus_tag="ClothDRAFT_2489" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.989) with cleavage site probability 0.857 at residue 26" gene 12105..13442 /locus_tag="ClothDRAFT_2490" CDS 12105..13442 /locus_tag="ClothDRAFT_2490" /inference="protein motif:PFAM:PF04055" /note="PFAM: Radical SAM domain protein; SMART: Elongator protein 3/MiaB/NifB; KEGG: dvm:DvMF_2901 radical SAM domain protein" /codon_start=1 /transl_table=11 /product="Radical SAM domain protein" /protein_id="ZP_05430629.1" /db_xref="GI:256005672" /db_xref="InterPro:IPR006638" /db_xref="InterPro:IPR007197" /translation="MTKTEKKLAWVDGFIARIKPYVYVRLKDNVLIRMPNEAFKLNTT GARVIDHILKGGSVYDFVKIRPDARETEMQLELFFVDFLRVWNKEVCENYTTAAVHRV EFSKGYIELPVLSEVALTYACNIKCRFCYAGCTKVKKNRELDKEGFKKVLDIIRYEAE VPSVSFTGGEPLLNKNLPELIKYASKANGMRVNLITNGTLITTKKAKELARAGLASAQ VSIESPYASEHDSITGVPGSHEASISGFKALKAAGVAVHPHFTICRLNQKSAVEYPEF CKEIGSDRFSANLIIPAGRGDDAELTIRYRDIGKIVEDIRKKALEAGVKFMWYSPTPL CLFNPIPVGLGNKGCSACEGLLSVDPEGNVLPCSSWAEPVGNLLDEGFEAVWFKKRSK WIRDKEAAPEKCKDCKHFAVCQGACPLYFNIHGYEELYDSWKSFGLCKERSSV" misc_feature 12402..13379 /locus_tag="ClothDRAFT_2490" /note="Predicted Fe-S oxidoreductases [General function prediction only]; Region: COG0535" /db_xref="CDD:30881" misc_feature 12453..>12890 /locus_tag="ClothDRAFT_2490" /note="Radical SAM superfamily. Enzymes of this family generate radicals by combining a 4Fe-4S cluster and S-adenosylmethionine (SAM) in close proximity. They are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster...; Region: Radical_SAM; cd01335" /db_xref="CDD:100105" misc_feature order(12471..12473,12477..12479,12483..12485,12489..12497, 12603..12605,12609..12614,12687..12695,12762..12764, 12885..12887) /locus_tag="ClothDRAFT_2490" /note="FeS/SAM binding site; other site" /db_xref="CDD:100105" gene 13439..14434 /locus_tag="ClothDRAFT_2491" CDS 13439..14434 /locus_tag="ClothDRAFT_2491" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430630.1" /db_xref="GI:256005673" /translation="MSGLSRIPGFPSPEREDSLKFYVLNSLPYSARMIIYLLLVAFGF LIQYITMNPWPGAFLLICATILGFVRGFDGHDRIKGFKTDKNWTTVDMDRIHEIRNLD YAITKWDRDVFDISNASGALVFVFFMAVLFVLSLIMFTIPGCFRVGMILITDSAVLVL PVWFSGIRRVFKQRKLMIKIDIIQDMEKYFATVRKEGEHFKPALLLARNRKGECMPKD ARFTVFFDGMPADFYGVQAQININEVQGGYYPYFYCVIPAKTGFGLREFLNRIQKGKN IVVEFQEDGQAEVIVIRQYTTKTSGYHTDTKSCKNILVTALAAARIILEAKRNGL" gene 14450..15040 /locus_tag="ClothDRAFT_2492" CDS 14450..15040 /locus_tag="ClothDRAFT_2492" /inference="protein motif:PFAM:PF00583" /note="PFAM: GCN5-related N-acetyltransferase; KEGG: hypothetical protein" /codon_start=1 /transl_table=11 /product="GCN5-related N-acetyltransferase" /protein_id="ZP_05430631.1" /db_xref="GI:256005674" /db_xref="InterPro:IPR000182" /translation="MSRTSQLRLYNPTLETERLILRKLKISDDKDIFEYAKDPEVSRY VTWEPHKSIEDARAFINWNLERYNKGEIGEWAIELKETGRVIGSIGFVELDRINFCGT VGYALSRKYWGKGIMTEAVRRIIRFAFEEMGLNRVEAVHIPENEASGRVMQKAGMMYE GLLRQRMFAKGRFWDLKQYAIIKDDVQLQGLQQEIS" misc_feature 14483..15004 /locus_tag="ClothDRAFT_2492" /note="Acetyltransferases, including N-acetylases of ribosomal proteins [Translation, ribosomal structure and biogenesis]; Region: RimL; COG1670" /db_xref="CDD:31856" misc_feature <14603..15001 /locus_tag="ClothDRAFT_2492" /note="N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of an acyl group to a substrate; Region: NAT_SF; cl00443" /db_xref="CDD:185998" gene 15460..17991 /locus_tag="ClothDRAFT_2493" CDS 15460..17991 /locus_tag="ClothDRAFT_2493" /inference="protein motif:PFAM:PF00501" /note="PFAM: AMP-dependent synthetase and ligase; Xylose isomerase domain protein TIM barrel; KEGG: pca:Pcar_1963 acyl-CoA synthetase" /codon_start=1 /transl_table=11 /product="AMP-dependent synthetase and ligase" /protein_id="ZP_05430632.1" /db_xref="GI:256005675" /db_xref="InterPro:IPR000873" /db_xref="InterPro:IPR012307" /translation="MKIAFSTLGCPDFSWTDIYSMAKDFGFNGIEIRGLGKEIFAVKA QPFTESELPKTLKKLSELRLEIPCFSSGCCLKFSENAEKNYEEIVEYITLASKTGTPF VRVLGDLEPEPQGEVDDNVVIEALKKLAPIAEEKGVTLLVETNGVYSDTKRLCELLDN VASDAVAALWDVHHPYRFAGETPGKTVQNLGAYIKYVHIKDSVVENGKIHYRMLGEGD LPIDDIMMALRSINYEGYISLEWVKRWAADLDDAGVVFPNFANYMSRYIKKSEVRGRL FDNARKTGKYIWEKDTLIDLTFPQLLDRVVEEFPDQYAFKYTTTDYTRTYAQFRDDVD TFARSLIALGVKPGDHVAIWATNVPQWFITFWATTKIGAVLVTVNTAYKIYEVEYLLR QSDTHTLVMIDGFKDSNYVEIIKELCPELETAEPGKPLHIKRLPFLRNIITIESKQKG CISWDEAIALAEKVPIEEVQRRALAVNRHDVCNMQYTSGTTGFPKGVMLTHYNVINNG KCIGDCMDLSTADRMLIQVPMFHCFGMVLSMIACVTHGSTMCPIPYFSPKVALDCINR EKITVCNGVPTMFIAMLEHEDFKKTDFSHMRTGIMAGSPCPVKVMQDVVDKMNMKEIT IVYGQTEASPGCTQSRVDDPIEVRVNTVGRPLPGIECKIVDPQTGEELPDNTDGEFVA RGYNIMKGYYKMPEATAAAIDKDGWLHTGDMARRDENGNYKITGRIKDMIIRGGENIY PKEIEDFIYTHPKVKDVQVIGVPDKQYGEEIMAWVILKDGETMTAEELQEYVRSNMAK HKTPRYVKFVTEFPMNAAGKVLKYKMREMAVDMLSLHEANSIVTA" misc_feature 15493..16164 /locus_tag="ClothDRAFT_2493" /note="AP endonuclease family 2; These endonucleases play a role in DNA repair. Cleave phosphodiester bonds at apurinic or apyrimidinic sites; the alignment also contains hexulose-6-phosphate isomerases, enzymes that catalyze the epimerization of D-arabino-6-...; Region: AP2Ec; cl12060" /db_xref="CDD:187176" misc_feature 15523..16116 /locus_tag="ClothDRAFT_2493" /note="Xylose isomerase-like TIM barrel; Region: AP_endonuc_2; pfam01261" /db_xref="CDD:144742" misc_feature order(15571..15585,15673..15675,15682..15684) /locus_tag="ClothDRAFT_2493" /note="DNA interaction; other site" /db_xref="CDD:28903" misc_feature order(15775..15777,15886..15888,15970..15972,15979..15981, 16051..16053,16090..16092) /locus_tag="ClothDRAFT_2493" /note="Metal-binding active site; metal-binding site" /db_xref="CDD:28903" misc_feature 16336..17970 /locus_tag="ClothDRAFT_2493" /note="AMP-binding domain protein; Validated; Region: PRK08315" /db_xref="CDD:181380" misc_feature 16432..17940 /locus_tag="ClothDRAFT_2493" /note="Acyl-protein synthetase, LuxE; Region: LuxE; cl10450" /db_xref="CDD:186997" gene 18244..18474 /locus_tag="ClothDRAFT_2494" CDS 18244..18474 /locus_tag="ClothDRAFT_2494" /inference="protein motif:TFAM:TIGR02196" /note="TIGRFAM: glutaredoxin-like protein, YruB-family; PFAM: glutaredoxin; KEGG: nis:NIS_1512 glutaredoxin" /codon_start=1 /transl_table=11 /product="glutaredoxin-like protein, YruB-family" /protein_id="ZP_05430633.1" /db_xref="GI:256005676" /db_xref="InterPro:IPR002109" /db_xref="InterPro:IPR011767" /db_xref="InterPro:IPR011911" /db_xref="InterPro:IPR014025" /db_xref="InterPro:IPR017937" /translation="MDVIVYTTPTCPWCTRVKEYLDQKGVQYREVNVAADRNAAMEMI RKSGQRGVPVVDIDGNIVVGFDQGKIDSLIGN" misc_feature 18247..18465 /locus_tag="ClothDRAFT_2494" /note="NrdH-redoxin (NrdH) family; NrdH is a small monomeric protein with a conserved redox active CXXC motif within a TRX fold, characterized by a glutaredoxin (GRX)-like sequence and TRX-like activity profile. In vitro, it displays protein disulfide...; Region: NrdH; cd02976" /db_xref="CDD:48525" misc_feature order(18274..18276,18283..18285) /locus_tag="ClothDRAFT_2494" /note="catalytic residues; other site" /db_xref="CDD:48525" gene 18925..20055 /locus_tag="ClothDRAFT_2495" CDS 18925..20055 /locus_tag="ClothDRAFT_2495" /inference="protein motif:PFAM:PF00248" /note="PFAM: aldo/keto reductase; KEGG: pca:Pcar_1733 aldo/keto reductase family oxidoreductase" /codon_start=1 /transl_table=11 /product="aldo/keto reductase" /protein_id="ZP_05430634.1" /db_xref="GI:256005677" /db_xref="InterPro:IPR001395" /db_xref="InterPro:IPR017900" /translation="MQYRGLGKTGVKVSALGFGAMRLPQININGNTRVDEEKSIEMIH RAFELGVNYIDTAPGYCNGESEVVVGKALKGWRDKIYLSTKNPIENASGDDWRKRLEN SLKKLDTDYIDFYHMWGINWETYETKIDVKGGPLEAARKAKEEGLIRHISFSFHDKPE NLIKLIDTGNFETVLCQYNLLDRSNEKAIAHAKSKGLGVIIMGPVGGGKLGEPSETIK KLLPKKTVSCAEIALRFVLANPNVDCALSGMSTIEMVEENVRVASNDTPLTKEELEMI RASMEENKRMEDLYCTGCNYCMPCPVGVNIPLNFQLMNYHRVYKITDYARGQYSQIGK VEWYKGKPAHECIECGVCETKCPQKLEIRKQLKETARVLSVK" misc_feature 18925..20043 /locus_tag="ClothDRAFT_2495" /note="Predicted oxidoreductases of the aldo/keto reductase family [General function prediction only]; Region: COG1453" /db_xref="CDD:31642" misc_feature 18931..19758 /locus_tag="ClothDRAFT_2495" /note="Aldo-keto reductases (AKRs) are a superfamily of soluble NAD(P)(H) oxidoreductases whose chief purpose is to reduce aldehydes and ketones to primary and secondary alcohols. AKRs are present in all phyla and are of importance to both health and...; Region: Aldo_ket_red; cd06660" /db_xref="CDD:119408" misc_feature order(18979..18987,19087..19089,19102..19104,19177..19179, 19270..19275,19384..19389,19447..19449,19528..19545, 19609..19611,19660..19671,19684..19686,19693..19698) /locus_tag="ClothDRAFT_2495" /note="active site" /db_xref="CDD:119408" misc_feature order(19087..19089,19102..19104,19177..19179,19270..19272) /locus_tag="ClothDRAFT_2495" /note="catalytic tetrad; other site" /db_xref="CDD:119408" gene 20234..20461 /locus_tag="ClothDRAFT_2496" CDS 20234..20461 /locus_tag="ClothDRAFT_2496" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430635.1" /db_xref="GI:256005678" /translation="MPFLRNRKSRSSHSRRLRFILDVSVDRMRLEMFSSMSPKHNILS MIILPLRLPKARTLAINTSGTKQKKFIAKPF" gene 20766..23921 /locus_tag="ClothDRAFT_2497" CDS 20766..23921 /locus_tag="ClothDRAFT_2497" /inference="protein motif:PFAM:PF08757" /note="PFAM: Spore coat protein CotH; cellulosome protein dockerin type I; KEGG: sde:Sde_3876 hypothetical protein" /codon_start=1 /transl_table=11 /product="Spore coat protein CotH" /protein_id="ZP_05430636.1" /db_xref="GI:256005679" /db_xref="InterPro:IPR002048" /db_xref="InterPro:IPR002105" /db_xref="InterPro:IPR014867" /translation="MKKVISGAVVIALLFAIMIPARYFGAFAEASQTLFINEVMSSNV STIRDGDVTDPKHGSKGGAYSDWIEIYNAGPYDVDLTGYILADSSAEWVFPQGIVPAG GYLLVWASDKNMVAQDGQLHTNFKLSASGENITLKKPDGTIVDSVDIIGLGDDQSYGR KSDGASEFVVFINPTPGAANVYNAPGTSTLFINEVMASNTRTIRDGDVDDPKDGSKGG AYSDWIEIYNAGPYDVDLTGYILADSSAEWVFPQGIVPAGGYLLVWASDKNKVATDGG LHTNFKISSSGEALTLKDPDGNVIDILVTINLLDDQAYGRKTDGSSELVLLKPTPGTA NIYDPSLIPVSEPVFSHQGGFYTGAFKLELTTNEPGVKIYYTTDGSDPVPGKSGTIEY TSGINIKSRKGEANVLSMIQDISNDQWNRWRAPNGEVFKCTTIKAVAIRDDGARSKVV THSYFVDPQMNTRYTLPVISIVTDYDNFFDKSTGIYVNGNYENRGKEWERPVHIEYFE TDGKLGFSMDMGLRIHGGYTRKYPQKSFRLYADHNNDIGEIKYEIFPGLRGTGTGKKI KSFERLILRNAGNDWTGALFRDEMMQSLVSHLKIDTQAFRPCIVFLNGEYWGIYHIRE RYDDKYLKSHYGLDDDKVAILDVYQTPEVQEGDSSDVLAYTNDVINYLKTHSITEKST YDYIKTKIDIENYIDYYVAQIFFGNTDWPGNNVSIWRYKTDDGQYHPEAPYGQDGRWR WMLKDTDFGFGLYGKSPSHNTLAFAAGDIREGQANEEWAVFLFKTLLKNEEFRNEFIN RFADQLNTSFVPSRVISIIDDIVATLEPEMKEHTDRWPFIKLTATSPWDTTWSQEVNR IRNYANSRPSYVRQHILSKFRNNGVTGTALVTLNTDSTRGHIRINSIDIVSDTPAVTN PNRWSGTYFKGVPITLKAIPKEGYVFDHWEGINGSVEASSDTITVNLSNDLNVTAVFR PENETPDPEILYGDYNGDGAVNSTDLLACKRYLLYALKPEQINVIAGDLDGNGKINST DYAYLKRYLLKQIDKFPVQLK" sig_peptide 20766..20852 /locus_tag="ClothDRAFT_2497" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.995) with cleavage site probability 0.698 at residue 29" misc_feature 22326..23369 /locus_tag="ClothDRAFT_2497" /note="CotH protein; Region: CotH; pfam08757" /db_xref="CDD:149727" misc_feature 23721..23783 /locus_tag="ClothDRAFT_2497" /note="Dockerin type I repeat; Region: Dockerin_1; pfam00404" /db_xref="CDD:144118" misc_feature 23823..23885 /locus_tag="ClothDRAFT_2497" /note="Dockerin type I repeat; Region: Dockerin_1; pfam00404" /db_xref="CDD:144118" gene 23973..24755 /locus_tag="ClothDRAFT_2498" CDS 23973..24755 /locus_tag="ClothDRAFT_2498" /inference="similar to AA sequence:KEGG:Bd0821" /note="KEGG: bba:Bd0821 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430637.1" /db_xref="GI:256005680" /translation="MEKLAIEVFNRYEKKYLMDNDTYEKIKDILAEYMELDEYNKVNN TYYTICNIYYDTQDNQLIRHSVSKPRYKEKLRLRSYGVPSLDAKVYLEIKKKVNGLVN KRRTKLTLREAYEFAATGIKPKYQSYMNKQVLNEIEYILSVYDLEPKLYLAYDRIAYF GINNRDLRITFDTNIRSRRTDLRLELGDYGEPLLDEGVWLMEVKAEKSIPVWLSRMLS ENGLFKTSFSKYGTEYKKMILNNIAKENDNDCSTEYLMSRLA" misc_feature 24003..24665 /locus_tag="ClothDRAFT_2498" /note="Polyphosphate(polyP) polymerase domain of yeast vacuolar transport chaperone (VTC) proteins VTC-2, -3 and- 4, and similar proteins; Region: PolyPPase_VTC_like; cd07750" /db_xref="CDD:143622" misc_feature order(24003..24005,24009..24011,24576..24578,24582..24584) /locus_tag="ClothDRAFT_2498" /note="putative metal binding residues; other site" /db_xref="CDD:143622" misc_feature order(24003..24005,24009..24011,24015..24017) /locus_tag="ClothDRAFT_2498" /note="signature motif; other site" /db_xref="CDD:143622" misc_feature order(24006..24008,24171..24173,24180..24182,24612..24614, 24624..24626) /locus_tag="ClothDRAFT_2498" /note="dimer interface; other site" /db_xref="CDD:143622" misc_feature order(24015..24017,24123..24125,24165..24167,24174..24176, 24198..24200,24204..24206,24252..24254,24258..24260, 24273..24275,24279..24281,24432..24434,24438..24440, 24474..24476,24576..24578,24582..24584,24651..24662) /locus_tag="ClothDRAFT_2498" /note="polyP binding site; other site" /db_xref="CDD:143622" misc_feature order(24015..24017,24123..24125,24198..24200,24204..24206, 24252..24254,24279..24281,24432..24434,24438..24440, 24486..24488,24576..24578,24651..24662) /locus_tag="ClothDRAFT_2498" /note="active site" /db_xref="CDD:143622" misc_feature order(24015..24017,24123..24125,24198..24200,24204..24206, 24252..24254,24279..24281,24432..24434,24438..24440, 24486..24488,24576..24578,24651..24659) /locus_tag="ClothDRAFT_2498" /note="substrate binding site; other site" /db_xref="CDD:143622" misc_feature order(24123..24125,24198..24200,24252..24254,24654..24662) /locus_tag="ClothDRAFT_2498" /note="acceptor-phosphate pocket; other site" /db_xref="CDD:143622" gene 24719..25393 /locus_tag="ClothDRAFT_2499" CDS 24719..25393 /locus_tag="ClothDRAFT_2499" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: bba:Bd0818 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430638.1" /db_xref="GI:256005681" /translation="MLDGIFDVTAGIKLSFGNMTLALAVSFALGIIISLVYIKTHLKE HYSQNFALALAIIPSIIAMMIMLIGSNVARAFSLAGAFAIIRFRSEEATFKDIAYVLF AMGAGLACGVGFLGYATLFTLVLCGFLIVLGKVNFGAKKSPYKVLKVTVRDDFDYKRE FEEVFNKYALSYELKRVKAKDEEGLYVLEYLVSLYYDVDEKEFIDALRYRNGNLNIAL GMNVEG" gene complement(25439..26338) /locus_tag="ClothDRAFT_2500" CDS complement(25439..26338) /locus_tag="ClothDRAFT_2500" /inference="similar to AA sequence:KEGG:HP0292" /note="KEGG: hpy:HP0292 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430639.1" /db_xref="GI:256005682" /translation="MLDFKPIELKDRELFHEYLKDYDFLTYEYSFLTLYIWRKMYNTE FAIVDDAIVIKKRTANNGTYFMQPIGADKSKIADITLKLNTLRKNNPDFKYLYGDVET PFLEQLHENFGNLVTSHEDKNNFDYIFNSKDLIKLSGKKYHRKKNQYNQFIKKYDYRI EEIQSPEVIKNCIDLSLKWYDYKSLQSEQLKNEQKAIFDIFSNIKIFNNIKGIAVYVN NEIAGFAIGEKLNSKMATVHFEKGNYNFSGIYPFLNKSLVEIFFRDVEFINLQEDLGL EGLRRAKSAYQPIKLEKKYLVNI" misc_feature complement(25448..26275) /locus_tag="ClothDRAFT_2500" /note="Uncharacterized conserved protein (DUF2156); Region: DUF2156; cl12090" /db_xref="CDD:159750" gene 26986..27834 /locus_tag="ClothDRAFT_2501" CDS 26986..27834 /locus_tag="ClothDRAFT_2501" /inference="protein motif:PFAM:PF07833" /note="PFAM: copper amine oxidase domain protein; cell wall hydrolase SleB; KEGG: hypothetical protein" /codon_start=1 /transl_table=11 /product="copper amine oxidase domain protein" /protein_id="ZP_05430640.1" /db_xref="GI:256005683" /db_xref="InterPro:IPR011105" /db_xref="InterPro:IPR012854" /translation="MSNEGRCKTGNFKAVLAVLIVIFVTLSGSFKASAINKQIFITVK VNGNYIKMDTWPYIKDGRTFVSVRFVAEALGAKVEWDGEDKKVTIKDDYNYIELFVGS KECYINAVKQAMDTSPEISNGRTMVPLRFVSEVLGCSVDWNQTTYTVSINKNGIKVPE ECIYQRNYTDEDLIWLARIVTVEAGGMSLEGKLAVANVVLNRVKSPSFPNTVYDVIFQ KGQFPPAYKSGFKELVPPDECIIAAKMALEGVNNIGNCLYFNNRPFTSRSGTFYKKIE GEYFYY" sig_peptide 26986..27090 /locus_tag="ClothDRAFT_2501" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.906) with cleavage site probability 0.750 at residue 35" misc_feature 27160..27438 /locus_tag="ClothDRAFT_2501" /note="Copper amine oxidase N-terminal domain; Region: Cu_amine_oxidN1; pfam07833" /db_xref="CDD:149091" misc_feature 27538..27804 /locus_tag="ClothDRAFT_2501" /note="Cell Wall Hydrolase; Region: Hydrolase_2; pfam07486" /db_xref="CDD:148857" gene complement(28003..30126) /locus_tag="ClothDRAFT_2502" CDS complement(28003..30126) /locus_tag="ClothDRAFT_2502" /inference="protein motif:TFAM:TIGR01525" /note="TIGRFAM: heavy metal translocating P-type ATPase; ATPase, P-type (transporting), HAD superfamily, subfamily IC; cadmium-translocating P-type ATPase; PFAM: E1-E2 ATPase-associated domain protein; Heavy metal transport/detoxification protein; Haloacid dehalogenase domain protein hydrolase; KEGG: wsu:WS1121 heavy metal-transporting ATPase" /codon_start=1 /transl_table=11 /product="heavy metal translocating P-type ATPase" /protein_id="ZP_05430641.1" /db_xref="GI:256005684" /db_xref="InterPro:IPR001366" /db_xref="InterPro:IPR001757" /db_xref="InterPro:IPR005834" /db_xref="InterPro:IPR006121" /db_xref="InterPro:IPR006404" /db_xref="InterPro:IPR006416" /db_xref="InterPro:IPR008250" /translation="MKQAIKKELILEGLNCANCAQKIETKVREIEGVKNASVNFVSKK MILELDDTLEPANIIKQASLIAKSIEEDIEVIEKQDKTNEKLKSNDNDFDKKELVKLG VGILLFVAALSLNLNYWVEFGLFFASYIIIGGEVLLKAFRNISKGKVFDENFLMGIAT IGAFLIGEFPEGVAVMIFYQIGEYLQNMAVNRSRKSIAALMDIRPDYANLKVGNSIKK VSVEDIKVGDIIVVKPGERIPLDGKVVEGRTMLDTSALTGESVPREVYPGEAVLSGTV NQTSLISVEVTKLFGESTVSKILDLVQNASSRKAPTENFITKFAGYYTPAVVFISAAL AVIPPLVVPGAGFFDWVYRALIFLVISCPCALVISIPLGFFGGIGGASKNGILIKGSN YLEALNSVDTVVFDKTGTLTKGVFNVTEVNPSSDFTKEDLLKYAAYAESFSNHPIALS ITKAFGKTIDKSKVESYEEIAGHGIKAVYHGKEILAGNTKLMAKAAISFANIETSGTV VHVAVDGIYAGNIVISDEIKADSKKALKELKEIGVRKLVMLTGDNNSTARKVASELEI DEVHAELLPHQKVEVVELLDGSKSKGGKLVFVGDGLNDAPVLARSDIGVAMGGLGSDA AIEAADVVLMTDEPSKLVTAIKIAKRTKTIIWQNIIFSLGVKVLVLLLGAAGIATMWE AVFADVGVALLAVLNAMRVMRIKNI" misc_feature complement(28015..30111) /locus_tag="ClothDRAFT_2502" /note="Cation transport ATPase [Inorganic ion transport and metabolism]; Region: ZntA; COG2217" /db_xref="CDD:32399" misc_feature complement(29947..30105) /locus_tag="ClothDRAFT_2502" /note="Heavy-metal-associated domain (HMA) is a conserved domain of approximately 30 amino acid residues found in a number of proteins that transport or detoxify heavy metals, for example, the CPx-type heavy metal ATPases and copper chaperones. HMA domain...; Region: HMA; cd00371" /db_xref="CDD:29471" misc_feature complement(order(30070..30072,30079..30087)) /locus_tag="ClothDRAFT_2502" /note="metal-binding site" /db_xref="CDD:29471" misc_feature complement(28939..29607) /locus_tag="ClothDRAFT_2502" /note="E1-E2 ATPase; Region: E1-E2_ATPase; pfam00122" /db_xref="CDD:143896" misc_feature complement(28228..28584) /locus_tag="ClothDRAFT_2502" /note="Haloacid dehalogenase-like hydrolases. The haloacid dehalogenase-like (HAD) superfamily includes L-2-haloacid dehalogenase, epoxide hydrolase, phosphoserine phosphatase, phosphomannomutase, phosphoglycolate phosphatase, P-type ATPase, and many others...; Region: HAD_like; cl11391" /db_xref="CDD:187016" gene complement(30128..30505) /locus_tag="ClothDRAFT_2503" CDS complement(30128..30505) /locus_tag="ClothDRAFT_2503" /inference="protein motif:PFAM:PF01022" /note="PFAM: regulatory protein ArsR; SMART: regulatory protein ArsR; KEGG: wsu:WS1120 putative transcriptional regulator" /codon_start=1 /transl_table=11 /product="transcriptional regulator, ArsR family" /protein_id="ZP_05430642.1" /db_xref="GI:256005685" /db_xref="InterPro:IPR001845" /translation="MDEKRDSCECNVIHEDIVNKVKKNMPKEEHLYDLAELFKVFGDT TRIKILYALFASEMCVCDIAALINMTQSAVSHQLRVLKQARLVKYRKEGKVVYYSLDD DHIKQIFDQGYMHITENESGKQV" misc_feature complement(30197..30394) /locus_tag="ClothDRAFT_2503" /note="Arsenical Resistance Operon Repressor and similar prokaryotic, metal regulated homodimeric repressors. ARSR subfamily of helix-turn-helix bacterial transcription regulatory proteins (winged helix topology). Includes several proteins that appear to...; Region: HTH_ARSR; cd00090" /db_xref="CDD:28974" misc_feature complement(order(30251..30253,30341..30343,30350..30355, 30362..30367,30374..30376,30383..30385,30389..30394)) /locus_tag="ClothDRAFT_2503" /note="dimerization interface; other site" /db_xref="CDD:28974" misc_feature complement(order(30212..30220,30233..30241,30257..30262, 30266..30271,30278..30283,30287..30298,30323..30331, 30368..30376,30386..30391)) /locus_tag="ClothDRAFT_2503" /note="putative DNA binding site; other site" /db_xref="CDD:28974" misc_feature complement(order(30320..30322,30329..30331)) /locus_tag="ClothDRAFT_2503" /note="putative Zn2+ binding site; other site" /db_xref="CDD:28974" gene 30754..33216 /locus_tag="ClothDRAFT_2504" CDS 30754..33216 /locus_tag="ClothDRAFT_2504" /inference="protein motif:PFAM:PF03422" /note="PFAM: Carbohydrate binding family 6; FG-GAP repeat protein; cellulosome protein dockerin type I; KEGG: cja:CJA_3559 rhamnogalacturonan lyase, rgl11A" /codon_start=1 /transl_table=11 /product="Carbohydrate binding family 6" /protein_id="ZP_05430643.1" /db_xref="GI:256005686" /db_xref="InterPro:IPR002048" /db_xref="InterPro:IPR002105" /db_xref="InterPro:IPR005084" /db_xref="InterPro:IPR013517" /translation="MKKTLVFLTALSLIFTLFISYSLSAGPASTKYGDLNADGKINST DYNLGKRLILRTISELPISNGSVAFDLNGDSKVDSTDLTALKRYLLGVIDKFPVGTDI PSQTQKTRYQAEDAMLYKAFEETIHAGYDGRSYVNYDNEPGGYIEWNVNVSSSGTYKL IFRYANGSNNNRPMEIRVNSNLVAGSLDFYPTSAWTVWNDQSIVVTLNAGNNVIRATG IASDGGPNVDYLEVIPTNEPPAPTPSPTPTVGPTPTGARQMERLDRGLVAVKVNNGVF LSWRMFGTDPSNIAFNLYRNGTKINSTPITGATNYVDTGGTTSSTYTVRAVINGQEQE ASKPVSVWAQNYLQIPIQPPSSAYEANDCSAADLDGDGEYEIVLKWEPNNAKDNSQSG YTDNVYLDAYKLNGTRLWRIDLGRNIRAGAHYTQFMVYDLDGDGKAEVACKTADGTRD GKGNVIGNPNADYRNSSGYILSGPEYLTVFDGQTGAAITTVDYDPPRGNVSSWGDNYG NRVDRFLACIAYLDGQRPSLVMCRGYYTRSVLVAWDFRNGRLTKRWVFDGNNYSGYNG QGNHNLSVADVDGDGRDEIIYGACTIDDNGRGLYTSGLGHGDALHVGDLNPNRPGLEI WSCFESSGGAALRDARTGEVLFRWHRSSDTGRACAADITASSPGAELWAAGSPLFSCT GQNIGTAPSQINFAIWWDGDELRELLDGITISKYGVGTLFTATGCASNNGTKSTPCLQ ADLLGDWREEVIFRTSDNRYLRIYTTTATTNRRIYTLMHDPVYRLGIAWQNVAYNQPP HTSFFIGAGMAEPPKPNIYLVP" sig_peptide 30754..30828 /locus_tag="ClothDRAFT_2504" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.872 at residue 25" misc_feature 30961..31023 /locus_tag="ClothDRAFT_2504" /note="Dockerin type I repeat; Region: Dockerin_1; pfam00404" /db_xref="CDD:144118" misc_feature 31087..31449 /locus_tag="ClothDRAFT_2504" /note="Carbohydrate binding module (family 6); Region: CBM_6; cl02697" /db_xref="CDD:155061" gene 33494..35299 /locus_tag="ClothDRAFT_2505" CDS 33494..35299 /locus_tag="ClothDRAFT_2505" /inference="protein motif:PFAM:PF00488" /note="PFAM: DNA mismatch repair protein MutS domain protein; SMART: DNA mismatch repair protein MutS domain protein; KEGG: scl:sce1459 MutS-like mismatch repair protein, ATPases" /codon_start=1 /transl_table=11 /product="DNA mismatch repair protein MutS domain protein" /protein_id="ZP_05430644.1" /db_xref="GI:256005687" /db_xref="InterPro:IPR000432" /translation="MNTKQMYLKRMRQYSDNAEKLERRSGMYSTIRLVTFAAGTFFTV LAFMYLSNVHGFISMGVFFVLFLFLVAKHQKVIDETLKYRTLAEINKRCVARMEGTWT EFEDKGEEYADPNHMYSKDLDVFGHGSLFQWINTTNTFFGRERLRRLLEFPEKEAGQI RKRQNAVKELSKKIDFCQSLQCEGMMAQNASKNPDKLLDFCEDGTKLFRNKLAEQLFY ILPELTIVFLVICWLDSSVSLYIPFFLLGVQAVINIVLHGRVSSILGPVSRYKNEIKV FYKMIELIEKKEFEDEYLIELKSRLFDKKKPASEQIKDLEKIVEATDIGKGYIVEILL NFFLFWNIHCVFALERWKAKAGKAIRIWLETIGDFEALASLALVAQMNPEWAFPEISD RKVCFNAVDMGHPLINEGKRVCNSINMDNKICIVTGSNMSGKTTLLRTVGVNLLLAYA GTAVCAKKMTCSVMDICTSMRVVDDLNEGISTFYAELLRIKMIIDHSRMKKPMIFLID EVFRGTNSLDRVTGARNVLLNLDKDWVIGMISTHDFELCNLEKGREGRIVNYHFAETY TNNEIKFDYILRRGQCKKSNARYLMKMVGIELLDE" misc_feature <33776..35281 /locus_tag="ClothDRAFT_2505" /note="Mismatch repair ATPase (MutS family) [DNA replication, recombination, and repair]; Region: MutS; COG0249" /db_xref="CDD:30598" misc_feature 34697..35281 /locus_tag="ClothDRAFT_2505" /note="MutS-like homolog in eukaryotes. The MutS protein initiates DNA mismatch repair by recognizing mispaired and unpaired bases embedded in duplex DNA and activating endo- and exonucleases to remove the mismatch. Members of the MutS family possess C-...; Region: ABC_MutS-like; cd03283" /db_xref="CDD:73042" misc_feature 34775..34798 /locus_tag="ClothDRAFT_2505" /note="Walker A/P-loop; other site" /db_xref="CDD:73042" misc_feature order(34784..34789,34793..34801,34904..34906,35015..35020, 35117..35119) /locus_tag="ClothDRAFT_2505" /note="ATP binding site; other site" /db_xref="CDD:73042" misc_feature 34895..34906 /locus_tag="ClothDRAFT_2505" /note="Q-loop/lid; other site" /db_xref="CDD:73042" misc_feature 34919..34960 /locus_tag="ClothDRAFT_2505" /note="ABC transporter signature motif; other site" /db_xref="CDD:73042" misc_feature 35003..35020 /locus_tag="ClothDRAFT_2505" /note="Walker B; other site" /db_xref="CDD:73042" misc_feature 35027..35038 /locus_tag="ClothDRAFT_2505" /note="D-loop; other site" /db_xref="CDD:73042" misc_feature 35105..35125 /locus_tag="ClothDRAFT_2505" /note="H-loop/switch region; other site" /db_xref="CDD:73042" ORIGIN 1 tcagccatac tgaaaacgta ttctattttt ggtatgagaa tatttattgc acctgatgta 61 gtaagtacaa attggagtag gatcgtcagg aacggaaaaa gaccagatga aattttattt 121 gaatactgaa aggagaaaat aagtatggca attataccaa gcgttacaga aaaatgtact 181 ggtgctatgt tggcgtctgc aattggtgat gctttaggtt ggccaaatga acttggagca 241 aaaaattcta taaaaaatac taaggtcaac gatcaattca ttgaatggac gcgacgttct 301 ggtggacgat attggcctta ttatgaaaaa atactacctg gtgagtatag tgatgatact 361 caaatgattt tagcagttgc acggagtgtt ataacaggta attgggaatc tactttagca 421 tttaaggaaa tccctttttg gctgaaatat gaacgtggcg gtggtagtgc tttaaaagag 481 gctgcaaaat tacttaaaaa gggaatagtt ccttgggaag caagagaaaa caacaaatat 541 tttatggctg gtggtaatgg cgcagctatg cgtattttgc cacatgtaat tcataagcaa 601 aatattgatt tacttatatt ggatgtaata aaagattgct tatatacaca tgggcatcct 661 agagccattt taggcgcttc atgttatgct tatgcgcttt attatttgag aaataaaaaa 721 gatgttttag agtatggaga gcttattaga gctgtaattg atggtttaaa atattggacc 781 actttaaatg cacttcctgc acaatggctc gttttggcag aaaagcattc tggatttgat 841 tatcttcaga tttggtttaa cactatttca aatatggcac agaaactgga atatatattt 901 tattcattag ataaaggatt acttattaat gatgatgatg tgctaaaata tcttggatgt 961 tttgatgaaa ctagtggagc cggagatgtt gcaattcttt cggcactata tttagcatca 1021 aaatacgcaa ataatcctat gctcggaatt aagattgcag caaatttgca taatgcagat 1081 actgacacaa ttgcgtctat tactggcgga ttattaggta tgttatgtgg tacggaatgg 1141 attccgtttg aatggagatt agttcaagat tatgattgtc ttgttaatat aacacaaatt 1201 ttactagctg ataatatgat agagacctca aggcatatta gtaacaagaa taaagtccag 1261 cataatgatt ggaaggattc acctataggc aagatgcgga ttcttactag gacagttgtg 1321 cctgctggta aatcacgaag tgttattatt gataagtttg aaactacatt agggcaaaca 1381 ctgtatatta aagaatttaa ggataatcga ataattgatt cttctcaaaa agaacaagaa 1441 aataaaactc aaactattat taacaagaaa gatatacatc attcaagttt tttaattgat 1501 agtaataata tacaggaatt acttgataat cccttagtag gacgtatatc atttaaaaaa 1561 gttttaaaaa ttattgattt attaataaac agtgatctaa gttgttctga agttgctaat 1621 aaattgaata tatgtgaaga agttataaga ctgataaaaa tgcagcttag gtagaattct 1681 aagctgatgc ataaaaaatg agtttaaatg gggtgaactg tatgtatatt tcaaaaattc 1741 agataaaaaa ctttagaaac tttaaacagt ttgaggcgca ttttaataat ggtataaatg 1801 ttataattgg ccataataac tctggaaagt ctaatttgat taaggcattg gctttaatat 1861 ttgattcgaa tacaaaaaaa cacttggaaa tcgatgactt caataaatat atttctctcg 1921 aggatttaaa aaacgagcca cctaaaattt ctatagctat taccattaca cagcaagaag 1981 atgaaaattt gatgtctgat gatttggtta ctataagcaa ttggttgacc aaattagaag 2041 agccatatga ggctttattg acatatgaat ttatgttgcc agttaaagaa catgaaagat 2101 ataaaaagaa agttaataat ttggaaaaag aagaggaaat ttgggaagta attaatagag 2161 atttcttacg tttatataca tataaaatat ggggtggtga tccaattaat aaaacagtgg 2221 ctgacagtga atctctgcaa aaatttgatt ttcaattttt gaatgcaatt cgagatgtag 2281 aaagagatat gtttacaggg cgaaacactt tactaaagag tgtattagat ttttttatag 2341 attatgatat aaaatctgat aaagaaaaaa cagaagatga aaagttatca gagattagtg 2401 aaataaagcg taaatttagt attgatgcaa ataatttaat taaaagtctg caaaaaagga 2461 tggaaaaggg caaaaaagaa attctttcct atgcatataa tataggtgct tcctttgata 2521 attcttatcc gaattttgat ggaaatatat cagatgtaca gctttattct gcattgagcc 2581 taattgttga gtacaaaaca ggaattaaga ttccagtttc tcataatgga cttggataca 2641 ataatttgat ttttatggct ttgttattag caaaaatgca gattaattca gatggaacat 2701 atctgggaag taatgcaaaa gtatttccaa ttttagttat tgaagaacct gaggcgcatt 2761 tacatccatc tatgcaatat cagcttttaa aatttttaaa tacaaatata aagcaaaata 2821 aggttagaca aatatttgta accactcatt caacacatat agcagcatca gtttctttag 2881 atcaaatgtt atgtctatat aaatataaaa atgatacaca aattgcttat ccaggaaaag 2941 tatttcctga tgaaaaaagt aaaaaatatg tgcaaaggtt tttggatgca acaaagtcag 3001 atatgctttt ttctgaaaaa gttattttag tcgaaggaat agcagaacaa ctcctaatac 3061 cgatttttgc gcgatacttg gaatgtccat tagaagaaaa acatgctacc gtaattaatg 3121 ttggaggtag atactttaat cattttttat atttatttga tgaaaataat ccatttgcaa 3181 taaaaaggaa agtggcttgt ttaacagata gagatccaga aagaaaagaa aaatctcaaa 3241 gcagatataa aaaatgttat ccttttgaat ttaatcttca acctgatact tatgattaca 3301 gatacaatac gttattagat aagtacgaag tgagagaaaa tcataaaaga atttgccaaa 3361 atattgcatc ttttaaacaa gataaaaata gttcaacttt tgaatatgac ctaatattat 3421 caaacccttc tttagatata ttagttacag agtcattgaa aaactctgaa gagataaaaa 3481 gtttaatgca agcatataaa aatggtgagc cacttgatga attttataaa ttattacgta 3541 ataacccaga aaataaacga attattgaat caattgaaaa agcgaactct aaagaatggt 3601 cagaagatga aaaaaagaaa gcaattattg cttctagata tttaaattct gttgaaaaag 3661 gagaaaatgc tttagaacta gcttatgtcc ttaaagaaaa cctagataaa aagggaacgg 3721 aagattttca agacttcaat gttcccacat atataattga agctataaag tgggtgtgtc 3781 aagatgatga taataaattc tgatactaga ataccaattg aaaaccattt tagagtttct 3841 gcaggtccag gcgctggtaa aacacattgg cttgtaaatc atataaaaaa tgtgttgcaa 3901 aattcaacaa ggttatctaa tgcaaggaaa attgcatgca taacttatac taatatcgca 3961 gtagagacaa tactaaaacg tttgggaaca gcagctgatc aagttgaagt atcaacaatt 4021 catagttttt tgtatagaca tgtagtaaag ccatattgtt cgtttattgc ctctgatgta 4081 ggtttgaatt tggaaaagtt taatggtcac aatgatacta ttgttagtaa ttctagaata 4141 aaacattggt tagaaaatct tccttgcaaa gatcagttaa aaaagccttg tgaatgtaac 4201 cagcttttat attcgaaagt ccataggaaa gcactgatta agtggctcga aacattatat 4261 tatagctttg ataatgataa gttatacata actggagata gtaaaaaggc atattacggg 4321 aaattgcatt tgactaatca atgtttgaaa atactcgaat cagaattatt tcaatataag 4381 aaattatatt ggaaagacgg tatagttgac cataatgata ttctatattt tagttataag 4441 cttatagaaa aatatccttt tataatacaa atactaagag ctaaattccc atactttttt 4501 attgatgagt ttcaggatac aaatcctata caactttctt taataagaaa aataggccaa 4561 aaggagacta tagtaggggt tattggcgat aaagcccaat ctatatattc atttcaaggt 4621 gctagtatga cacaatttga aaaattttat cttccaaatt taattgatta tgttatggta 4681 gacaatcgta gaagtacaaa tcaaattgtc gatgtattga atttaattcg gaaagatatt 4741 cagcaaaata aatttcgcaa ggtagaaggt gatttgccag ttattttagt tggtgataga 4801 aataaagggt atgaaaaagc aaaagagtta tgcaataata gtgaaatctg tactttaact 4861 agagacaatg caactgcgaa tagtatgaaa aaggaaattg atgaaaatat ttcagataaa 4921 aatttatttc ttgaactaaa aatcctagat agtaattcaa aaaggtgcaa tgtagtttat 4981 tcatgtatac aagcagttga attagcaaat aatggtaatt acaaagaagc tataaaaaag 5041 ctagaatatt tatttacaga aaggaatgat aaacacgcag ttaaaaagat ggctttgagt 5101 tgtttatgtt tgttaataaa taactataat agttttaaag ataaaacttt aatggatttt 5161 tacagctttg ttaaagataa aatctttaca gatttatcaa agtttcggaa aggaaatatt 5221 aaaacttttt atgaaaaaca tacataccaa cagttggccg catatgtaaa tatagtagat 5281 gatattagtt actttaaaac tattcataaa gcgaaaggtg atgaatttaa taatgtttta 5341 ctcatattaa aagaaaaaga tttagatttt ttattgaagc ctaatcttct taaaaatgaa 5401 gaacatagga tttattatgt tgcaattagt agagctaaag agaaactatt tatttgtgtt 5461 gataatttaa atccgcaaaa tattttaaag ttaaatacac tctttaatat tgttatttta 5521 aattgataaa atttactgaa tagggaatgt agatatttct gtggtgcagc ggtcaagggc 5581 gtgtagcaca gtgagtggaa accctatcgg gtaaaccttt agctaaggaa ctcatggtga 5641 gtccttgaag ggactatctc aaaatagatt tttgaaatag atccttgttt gttaaaaaat 5701 acaaaaatca ggtgcaatat cctgccttct gttgtaaaat atagttataa attaaatact 5761 atttcaaaaa ggttatacta taaatagcgg aacagttcaa ttatctacta ttagaaatag 5821 aaattcttgt actggcaaat gattctgtga gactgctaag ccaaggacca tgttccaaat 5881 gcgcgtggaa tttgaggttg tggccccgac aatgtttttg gtaaattatt gtttggagaa 5941 aatcaatcat aaaaaggagt cgccacaatg gaattatatt atgttaacac gaagaagaaa 6001 acaaaaatga attatcaaag tatcatattg aaattaatga agtccacatg ttggcaagaa 6061 acggtcttct tgctttatca atagctgctg gaatacaggt cctaaaaaca atgttagatg 6121 tagaagttga agagaaagca ggacctaaag gaaagcacaa caaggacaga accgtataca 6181 gctatggaac tgaaaaaacg tctgtggttt tggacgggcg aaaagtaaaa atagaacgtc 6241 caaggttatg cgtatacaaa ataaggaagt taactttgaa agactcgaac agtttcagtc 6301 gtaggacgcg ttaaatttta ggatatttaa gcaaatacta atgggaataa caacaaggaa 6361 ctataacgaa tgtcaggata cagttcctgg tatagagaca tttagagcag gcaaaagcaa 6421 tgctagccgc cgatttgtgc aagaaacaag taaacgtatg aaagaattta ttagccgcgg 6481 gcttgatgat attcatataa taataattgc tacacggagt aggatttgga gaatatcatg 6541 tattggtttc acttcgagtg aagagtacag gagaaaaaag gtattaggaa taaagggagc 6601 agtgagaacg tagaagtgtg caagaatttt ataacatata tctcataaaa agggtgatag 6661 agccgaaatg gcaacagatt atttatattt gaaggttcaa aagcgttatt aaaggcagta 6721 aaggagactt ttaagaacag agtgaaaatt aaagatgtta attgcataag aagtgaaatt 6781 tggaaagtta tttgctagac agtgagagaa gtaaagttat cagagaaatg aatgagacat 6841 acacagaatt taattataag gaaacgaaaa agaatttaga aggacttgct agttcactgg 6901 aacacaagta cctggtggaa gcgtcgagtc ttgatgaagg acttgacgag atattgacgt 6961 tgcatcgatt aaaagttccc ggccttttga ggatttcatt tttgacaacg aacttaatag 7021 agttagcaag agaaacagcg agggatatta tgggaagagt gaggggatgg agtaaaggga 7081 atcaagtttt aaggtggtta tcatgtgttt ttttacacgc gagaaacagt ttaagcaagt 7141 aaaaggctac aaattatcac ctgttcttat tagcgcttta ttccgcggag gaggaattac 7201 aacctacttt aataatacgc tggtaggatg agaaaaatca agaaaaaggg cacctgagaa 7261 attttacgaa agattggaca aatataatgc caaaatgtaa tatgactttc gagggaaaat 7321 tacgggagca ttatttgcgc catgacggga catattcgga tgcattagtt tatggtataa 7381 taaaaaatga atttagatac agcttctaag aagatgaatg agatttagta atattagcaa 7441 taattaaagg gaaaagaagt tgttttaagc tttagtaata atagtattta aggaggtaag 7501 ccatggataa cagaaaaaaa ttaatattag gaacagtaat ttttataggg gttatattaa 7561 taatcggttt tactttattt tcaacattaa cattggaacc gagtgtaaag gtaggttatg 7621 ttggaagtaa tgctggaaat acgattaatg cttcttttaa gtattttgac ggtgtggaaa 7681 gcagaaaggt taaattcaat gaaggtgacg ttgtaacaat aaaatatgct ataggattgg 7741 aagaaggaag tctcagttta gttgtttttg atgaaaaggg ggacgaaata atcaaaaggt 7801 cagataatga aggagaagta agttttgaag taactgaaac tcaaaagtat aaaatcagtt 7861 tggttgctgc caaggctaaa ggaaatttca gtgtaaagtg ggatgtataa tcagaaggga 7921 tgtatgacat gtacacataa aggggcaagt tattatcgaa ttagttaaat cacacacgaa 7981 atcatctatg tggttagtaa tatttagtga tattaaattc tatatttttc ctttttgtaa 8041 attttgcagg gattcatgcg tagtacagat gatatatcag tattttgatg ataatcgttg 8101 atagttttgt aacactgttg ttttctattg ttttaagtgt tttgacttta attgggaaag 8161 atgtatttgg taacttcttt atgcctgccg tggcttaaca taagggggta atgaatagtg 8221 aagctaaatg gctatgaatt gatatgtcca atctgtaaag ggaaaaaatt tgagaaaaga 8281 aacagcctgc ttaatacgag agggatgact tttttaaatc ttgattggtt aaatgaaggc 8341 gcaataaatt atatatgtga tacttgcggt tatattcttt ggtttattga tgacggaagg 8401 gaatatgtag aggaatatga tgaaaaagag aaaattggga taaaaggctt acatatagat 8461 tatgaaactt cgaaggcgga tgaggatgaa tgcccggttt gttttagtaa acgggatgta 8521 aatgataaag aatgttcgaa ttgcgggtat aaatttgaat aagattcttg agtttgttag 8581 tttctttttg taagctttgg gtataaaagg aaaaggcaaa agaactataa aataacgtat 8641 tatcaaaatg ttaatgagaa ttaatccaaa aaaggagaaa ttacatatgc ttaagcattc 8701 gtcaatatgt ttgtttttaa gttcggtttt tgtaatttta cttttttcag aatgtcagac 8761 aggaaacaac tgggaagttt cgcaacgctc tgttataatg atgcagagtt tttcattcat 8821 tgcgaagata ttctacataa taagtattac tatttttatt atgaatctta agaactatat 8881 taaaattaga aatagattga agttcataat gataatatgg tttctattat tggcagtgcc 8941 acttataata gtccagttaa atggcatccc ggttgattcg ttgagaatag ttggcactga 9001 gttttttaga cagtcatata ttgttgaact tgtgggaatg gggaccagtc tgatttttct 9061 tgttatattg cttattgttt ttcggggtag acaagcaatt gatgagacaa cggaatccaa 9121 ggattgattt ataggtaacc gataataaac cctttgcata ttaataatta tgcagcttaa 9181 acacagcaga gcattaagca aatgacttaa ctttgatgca aaggggatgg actgatggaa 9241 ggagtaatat tagtcaccgg tgcggcaggc tttatcggtt tccacttggt acagcgcttg 9301 ttaaaagagg gttgtaacgt cgtaggtata gataatttaa atgagtatta cgatgttaaa 9361 ctgaaaaaag accgcctgaa attgttaagt gaaaataaaa actttgtatt ccgcaaagtt 9421 gacataaaaa acaaaaaggc agtggaccgt atctttgaaa cctatcggcc ttcctatgta 9481 atcaatcttg cggcacaagc gggagtgcgt tattccattg aaaatcccta tgcctatgtg 9541 gattcaaatt tggtaggatt tgtgaacatt cttgaggctt gccgaaaata ccctgtgaag 9601 caccttatct atgcttcatc aagttcggta tacgggggaa acaaagtttc gccgttttcc 9661 accagacata atgtggacca tcctgtgtct ctttatgcag ccacaaaaaa atccaatgaa 9721 ttgctggccc atacctacag tcatcttttc ggcattccca caacagggct gaggtttttt 9781 accgtttacg gcccctgggg aagaccggat atggcgtatt tctcatttac aaaagatatt 9841 ttaagcggaa accccattaa agtgttcaat tatggtaaaa tggaaagaga ctttacttat 9901 attgatgatg tggtggaagg aattgtaaaa ttaattgaca gaatcccgac gcctaatgaa 9961 aactgggatg aaactaaaga tgacataagt accagttttg caccgtacaa aatctacaat 10021 atcggcaaca acaatcctgt tccgttaatg aatttcataa gtgttttaga gtcagctctt 10081 ggtaaggttg caaaaaaagt atatttggat ttgcaacccg gcgatgtgct cagaacctat 10141 gcggatattt ccgaccttga aagggatata aatttcaagc cgtccacaag tattgaagac 10201 gggcttcgaa aatttgtaca gtggtacaag gagtattata aagctgaaat ttagcaagaa 10261 atttttagag acaaacagcg taaaataagt taaaattata tttaacaaaa atttttattt 10321 ttttaaatag acttgtaaat gataaaaaag tccggatttt aaaatacgca tataatattt 10381 atgcatgcaa aaggagttaa tatgattaaa agatttatag tgtttttttc aattttactt 10441 gttttaatta taccgacgtg tgtaaaggtg gatgcgtccg atattgccaa tcttggtttt 10501 gttgaaaccg atgttatttt gcagcctgac ggcaaggcca ttgtctccta caccgtgcgt 10561 tacaacctgg tgccgggaaa gacaatgctg gcatttacca tgtcagggtt tgacaagctt 10621 agccctgttt ttgacacgga aaatgcatgg gttataactg acgacaacac ctcttatccc 10681 atcgatatag ttaatctcgg caatggttac tatgacatta taaattcagg caataaaagg 10741 ctgggaggag agtacctcac ctacaaattt cgttttgttg ccgatatggc tgcggccgga 10801 tatttgaaaa agaccactgc cgaggacgga cgaaggcttg tggtgtttga ctgggcccct 10861 acccaatggg aggaggcaat ggaacattat actgttaccg taaactatcc tttggctttc 10921 ccgaaagaat ccggcacaag ggaagaagtt gaaagttttc ttcttgagca tgactttgcc 10981 acggagaagt ggatgaatga aaaatacctt attgactaca gggttcaggt gctggatggg 11041 actccgagag ttcagatttt gcttcacaag gagaatttgg cggcttatga caagtttgaa 11101 attaaacagt acattacaga ggatattttt aatatagatg atccggcaga tattgatttt 11161 ccgccgaatg agtttgggaa agggcctttc gaccggtacg aagaaaatat ggacaggcag 11221 ccggagaatg tgaacaatgg ctctgaaggc agaataattt tattgacaat cttaatcgga 11281 ctttttgtaa tgatctacat aatagtggga aggaagcaca gttccgtgat gaaagcccgg 11341 gagactttgg atcagattca atgggcaagg caggattggg atcctccaaa gattgaaata 11401 gcttcgttca ggaaggacgg aactgttgcc accgggcttg atgccgttga gatagcactt 11461 ataataggga ttccctataa gaggattatt tcggttatta tttcaaaact tgtagagcaa 11521 gggtttcttg aggaaatttc ggaagaaccc ctgcgggtgc gccgcattga acatgacaat 11581 gcgtcagcgg tcctgaatga atacgaatta atggtgtatg aagctgcaaa agacggagag 11641 attgacgaga cggaaataga aaatataatt caaagattga cggataatgt gcggcaaaag 11701 acgtgggact gcgacattga ggccacccgg aagtactatg ccgataaaat gtccgagctt 11761 ttgaaaaagg aaaatgcgga cccggcacag gcggcagaca attcatatac cggagatgat 11821 tatatttacg cctggccgta ctggtattat ttccacagcg gtttggtgta tcacagacag 11881 agagaccgcc atgaaagata tgaaaggtct gtaccggtca atccgggcag gttgtcgttt 11941 aattcagctt tagaccccga cacaggcgct tttgcgtgcc attctgcgtg tcactcggca 12001 tgtcatgatg cgtgccattc ggcatgtcat tcggcatgcc attctgcgtg tcactcagcc 12061 tgccattcgg cttgtcactc tgcttgtgta agcgggggtg caagatgacg aagactgaga 12121 aaaaactggc ctgggttgat gggtttattg cccggataaa accgtatgta tatgtcaggc 12181 ttaaagacaa tgtgctgatt cgtatgccca atgaagcgtt taagctgaat accaccggag 12241 caagggttat tgaccacatt cttaaaggtg gttcggtgta tgattttgta aagatacgcc 12301 ctgacgcccg ggaaaccgag atgcagcttg aacttttttt cgtggatttt ttgcgggtgt 12361 ggaacaagga agtctgtgag aattacacaa ctgctgcggt acacagggtt gaattcagta 12421 agggatatat tgagcttcct gtcttgtcgg aagttgctct tacctatgcc tgcaatataa 12481 aatgccgttt ttgttatgcc ggatgtacca aggtaaaaaa gaacagggag cttgacaagg 12541 aaggatttaa aaaggttctt gacattatcc ggtacgaggc tgaggtaccc agtgtatcct 12601 ttacgggagg ggagcctctt cttaacaaaa atcttcccga acttataaaa tatgcctcga 12661 aggccaacgg gatgagagtt aaccttataa ccaacggcac gctgataact accaaaaaag 12721 caaaagagct tgccagggca ggtcttgcgt cggcgcaggt gagtattgaa tctccgtatg 12781 cttcggagca tgattccatt acgggagtgc cgggttccca tgaggcctcg atatcgggat 12841 ttaaagccct taaggcagca ggagttgcgg ttcatcctca ctttacgatt tgcaggctga 12901 atcaaaaatc agccgttgaa tatccggagt tttgcaaaga gataggttct gacaggttct 12961 ctgccaatct tattatacct gcggggcggg gcgatgacgc tgaattgaca attcgttatc 13021 gtgatattgg aaaaatagtg gaggatatac gaaaaaaggc tttagaggcc ggcgttaaat 13081 ttatgtggta ttctccaact ccactgtgct tgtttaatcc aattcctgtg gggcttggca 13141 ataaaggatg cagtgcctgt gaaggattgc tttccgtcga tcctgaagga aatgttctgc 13201 catgttcaag ctgggcggag cctgtgggga atttgctgga tgaaggcttt gaagccgtat 13261 ggtttaaaaa gcgctccaaa tggataaggg acaaggaagc cgcacctgaa aagtgcaagg 13321 attgcaaaca ttttgcggta tgtcagggag cctgtcccct gtatttcaat atacatggat 13381 acgaagagct ttatgattca tggaaatctt ttggtttatg taaggaaagg agtagcgtat 13441 gagtggcttg agtcgtatac cgggttttcc gtctccggaa agagaggata gtttgaaatt 13501 ttatgtgcta aatagtcttc catattcggc gaggatgatt atatatttac tgcttgtagc 13561 ctttggcttt ttaattcagt atataacgat gaatccgtgg cccggggctt ttttgttgat 13621 ttgtgcaacg attttgggtt ttgttcgtgg ttttgacggt catgacagga ttaaaggttt 13681 taaaaccgac aaaaattgga cgactgttga catggaccga attcatgaaa tcagaaatct 13741 tgattatgcg ataacaaaat gggacagaga tgtttttgac ataagcaatg cgtcaggagc 13801 tttggtgttt gtattcttta tggctgtttt gtttgtgctc tcactgatta tgtttacgat 13861 accgggatgt tttcgggtgg gaatgatttt aataacggat tcggctgtcc ttgtactgcc 13921 tgtgtggttt agtggaatac gacgggtttt taaacagcgg aaactcatga taaaaattga 13981 tattattcaa gatatggaga aatatttcgc aactgtcagg aaggaaggag aacatttcaa 14041 gcctgcattg ctgcttgccc ggaacagaaa aggagagtgt atgccgaaag atgcgcgttt 14101 taccgttttc tttgacggta tgccggctga tttttacggt gtccaggcgc agataaacat 14161 aaatgaggtg cagggagggt attatccgta tttctattgt gttatacccg ctaaaacggg 14221 atttggactt cgagagtttt taaatagaat tcaaaagggt aaaaatattg ttgtagagtt 14281 tcaggaagac ggacaggcag aggtaattgt cattcgacag tatactacga agacatcggg 14341 atatcacaca gacacgaaaa gttgcaaaaa tatacttgtt acggcccttg cggctgcaag 14401 aattatactt gaagcaaaac ggaatggttt gtaagaggtg actttgacaa tgagccggac 14461 atcacaactg cgtttgtaca atcccacatt ggagacggaa aggcttattt taaggaagct 14521 taaaatttct gatgataaag atatatttga atatgcaaag gatcccgaag tatccagata 14581 tgtaacctgg gagccgcaca agtccattga ggatgccaga gcgtttatca attggaatct 14641 tgaacgttac aacaaaggtg aaatcggcga gtgggccatt gagcttaaag aaactggaag 14701 agtaatcgga agtatcggat ttgtggagct tgacaggata aatttttgtg gaacagtggg 14761 atatgccctg tcaaggaaat actggggcaa gggtattatg actgaagcgg tcaggcgtat 14821 aattcgtttt gcttttgaag aaatgggatt aaacagagtt gaagcggtgc acattcctga 14881 aaatgaagca tcgggaaggg taatgcaaaa agcgggaatg atgtatgaag ggctgcttcg 14941 ccaaaggatg tttgcaaaag gaaggttttg ggacttaaag cagtatgcca ttataaaaga 15001 tgatgtccag ttacagggat tgcaacagga aatatcttaa taataatctc aatggaatta 15061 ttcttgacat ggaaaattta atctgataat ataacatcaa aaatggtaaa atgcgttgac 15121 agggagaaag cactgccata gcgtgttcca gagagctgtt ggttgctgca aaacagtaca 15181 cgggcaggtt gcgagctcgc ccttgagcag tcagctgaaa gcctgaaaag ctttttaatt 15241 ttcgggcaag taggtgtgaa cgggtttctc gccgttacaa cgagaaggca ttgatttggg 15301 tatatattct gtttcgatgc cgcaaagggg gtatttttat accaagaaga gtggtaccac 15361 ggaagaaact ggctttcgtc tctgacattt atgttatgag gtgaaggctt ttttaattct 15421 taaaagtttt taaatactgg tattatagga ggaaatatta tgaaaattgc gttttcaaca 15481 cttggttgtc ctgacttcag ttggacggac atttattcca tggctaagga ttttggattt 15541 aacggtatcg aaatccgtgg tcttggaaag gaaattttcg ccgtgaaagc acagcctttt 15601 accgaatcag agctgcctaa gactttaaaa aagctttcgg aacttcgtct tgaaattccg 15661 tgcttttctt cgggatgctg tttgaagttt tccgagaatg ccgagaaaaa ttatgaggag 15721 attgtagagt atattacgct tgcttccaaa acaggaactc cttttgtccg tgttcttggc 15781 gaccttgagc cggaacctca gggagaagtt gatgacaatg ttgttattga ggcactgaaa 15841 aaacttgccc ccattgcgga agaaaaaggt gtaacgcttc ttgtggaaac caatggtgta 15901 tattccgaca caaaacgtct gtgtgagctg cttgacaatg tggccagtga tgcagtggcg 15961 gctttgtggg atgtacacca cccgtataga tttgccggtg agactcccgg aaagacggtg 16021 caaaatcttg gagcatacat taaatatgta catatcaagg actcggttgt tgaaaacgga 16081 aaaattcatt atcgcatgct tggtgaaggt gatttgccaa ttgacgatat catgatggca 16141 cttcgttcaa tcaactatga aggatacatt tctctggaat gggttaaacg gtgggctgcg 16201 gacctcgacg atgccggagt tgtcttcccc aattttgcaa attacatgag ccgctacatt 16261 aaaaaaagcg aagtgagagg gcgcttgttt gacaatgcga gaaagaccgg aaagtatatt 16321 tgggagaaag acacgcttat tgatttgaca ttccctcagc ttttggaccg tgttgttgaa 16381 gagtttcccg accagtatgc cttcaagtat accacaaccg attatacccg gacttatgcc 16441 cagttcaggg atgatgtcga tacttttgca agatccctga tagctctggg agtaaaaccg 16501 ggagaccatg ttgccatctg ggctaccaac gtaccccaat ggtttattac attctgggcg 16561 acaaccaaga ttggagcggt gcttgtcacc gtaaacaccg catataaaat ttatgaggtt 16621 gaatatcttc tccgtcagtc ggatacccac acactggtta tgattgacgg atttaaggat 16681 tcgaattatg ttgaaattat taaagaactt tgccctgagc ttgaaacggc ggagcccgga 16741 aaacctctgc atatcaagag gcttcctttc ctgcgcaata tcattactat tgagtcaaaa 16801 caaaaaggct gcatttcgtg ggatgaagca attgccctgg cggaaaaagt gcctattgag 16861 gaggttcaac gccgtgctct tgcggttaac aggcatgatg tctgcaatat gcagtatact 16921 tcaggaacca ccggattccc aaaaggtgtt atgcttaccc attacaatgt tattaacaac 16981 ggaaaatgca ttggagactg tatggacctt tccactgccg accgcatgct gatccaggtt 17041 ccgatgttcc actgctttgg aatggtgctt tcaatgatag cttgtgtgac tcatggttcc 17101 acaatgtgtc cgataccgta tttttcaccg aaggtggctt tggattgtat taaccgtgag 17161 aagataaccg tctgcaacgg tgttccgacg atgtttattg caatgctgga acacgaagat 17221 ttcaaaaaga cagatttctc tcacatgaga acgggaatta tggccggaag cccgtgtcct 17281 gtaaaggtta tgcaggatgt ggtggacaag atgaacatga aggagataac cattgtatac 17341 ggtcagactg aggcttcacc gggctgtacc cagagccgtg tggatgatcc tattgaggtg 17401 cgtgtgaata ctgtcggacg tccgcttccc ggtattgaat gcaagattgt ggatcctcaa 17461 actggtgagg aattgccgga taataccgac ggagagtttg ttgcccgcgg atataatatt 17521 atgaaaggtt actacaagat gcctgaagcg acggcggcag caattgacaa agacggctgg 17581 ctccataccg gtgacatggc aaggcgtgat gaaaacggca actacaagat aaccggccgt 17641 atcaaggaca tgataatacg tggcggtgaa aatatttatc cgaaggaaat tgaagacttt 17701 atatacactc atccgaaagt aaaggatgtt caggttatag gtgttcccga caagcaatat 17761 ggtgaagaga ttatggcatg ggtaatcctt aaggacggcg aaacaatgac tgccgaagag 17821 cttcaggaat atgttcgctc caatatggca aaacacaaga cgcctcgata cgtcaaattt 17881 gttacggaat tccccatgaa tgcggcagga aaggtattaa agtacaaaat gcgtgagatg 17941 gcagttgaca tgttgtccct ccatgaagcc aattcaatcg ttacggctta aaccggatta 18001 actttttcaa acctcggtca ggcttccgtg gtgtgtttgt gtggagtctg accgggtgtt 18061 tttaaaagaa tgatttcatc aattcctgct tttctgttat gctgatgtga gcagcttacg 18121 tgatgtggat tgcacttcta taagtgaaaa aagagggtgt tttaaaagga aaaattgtgg 18181 aataattaaa tagtaagcag tagtcttatg gtggttagag taaataatta ggagggcgat 18241 aatatggacg taattgtata tacaacacct acatgtccat ggtgtacaag agtaaaggaa 18301 tatctggacc aaaaaggtgt tcagtacagg gaagttaacg ttgcagccga cagaaacgct 18361 gcgatggaaa tgattagaaa atccggccaa cgtggtgtac ctgttgtgga tattgacgga 18421 aacattgttg taggatttga tcaggggaaa atcgacagtt tgataggaaa ctgaaaatat 18481 agatttaccg ggtagggatt ggcacgtgta aaaacacacg aaaaaaggct gactgtctgg 18541 tgaaagacag ccggcctttt gtgttaatgc cgttccactt ttgtgaagtc cggctgaaaa 18601 gggagggagc ttaacgctca taaattttgc tgcttgcgca aagggtgtgc acgtttttgt 18661 tatttgaggg acttcattac tatagattcg cattgaagtg attttttgtg accgaattag 18721 taaaaaataa ataaaaaaat attttgatga aataaaaagt tatcaaaagg ttaaaggtgg 18781 agaagacaat atagacgggc ataatctctt ctgttaaaat gtaagaagat agttttcttt 18841 atttgtccca tattttgtta ttaaaattta tttttatata aattttatgt gggaaatgaa 18901 acattatgga ttggagggaa ttaaatgcag taccgtggat tgggaaaaac aggtgtgaaa 18961 gtatctgctc ttggctttgg agcaatgagg cttccacaaa ttaatattaa tggcaatacg 19021 agggttgacg aggaaaaaag tattgaaatg attcacagag cttttgaact tggagtgaat 19081 tacatagata ccgccccggg atactgcaat ggtgaaagcg aggttgtcgt aggaaaggct 19141 ttaaaaggtt ggagggacaa gatttattta tccaccaaga atccgattga gaacgcatca 19201 ggggatgatt ggcgtaaaag gctggaaaat tcattgaaaa agctggatac tgactatatt 19261 gatttttatc atatgtgggg tatcaactgg gaaacctatg aaacgaaaat agatgtgaag 19321 ggaggcccgc tggaggcggc acggaaggct aaagaagaag gtcttatccg tcatatatcc 19381 ttctcatttc atgacaaacc tgaaaatttg attaagctta tagacacagg taattttgaa 19441 acggtgctgt gtcagtacaa ccttcttgac agaagcaatg aaaaagcaat agcccatgca 19501 aagagcaagg ggctgggagt aataatcatg ggtcctgtag gtggaggaaa actcggtgaa 19561 ccctcggaga caattaaaaa actgcttccc aaaaagacgg taagctgtgc ggaaatagcc 19621 ttgcgatttg tacttgccaa tccaaatgtt gactgcgcac tgtcaggaat gagcacaatt 19681 gaaatggttg aggaaaatgt gcgtgtggca tcaaatgata ctcctcttac aaaggaagag 19741 ctcgagatga taagggcgtc aatggaggaa aacaaacgaa tggaggattt gtattgcacc 19801 ggctgcaatt attgtatgcc gtgtcctgtc ggtgtcaata ttccgcttaa ttttcagttg 19861 atgaattatc acagggtata taaaattacc gattatgcaa ggggtcaata cagccaaata 19921 ggcaaggttg aatggtacaa gggtaaaccg gcccatgagt gcattgaatg tggcgtgtgt 19981 gagacaaaat gccctcaaaa gcttgaaata agaaaacagc taaaagaaac ggcaagggta 20041 ttgtcggtta aatgaaatat ataatatgta ctttagttca tagcctgcgc cgcgaatggt 20101 atctattgtt actttggaat taatattgct tagttttttc cgtaaaaagg atatgtaaac 20161 ctcaacgtgg ttggcacttg ctttggaatc aaagctccac agtttctcga tgattgtttc 20221 ttttggagaa atcatgcctt ttctgaggaa cagaaaatcg agaagctcgc attcgcggcg 20281 tctcagattt attttagatg tttctgtgga cagaatgaga ttagaaatgt tcagttcgat 20341 gtcgccaaag cacaatatat tgtccatgat aatactgcct ttgcgccttc ccaaagcacg 20401 aactcttgcc atcaacactt cgggcacaaa gcaaaaaaag tttatagcaa agccttttta 20461 aggttgattt aagctttgca atatagaatg ataatatgaa aaatgtattt tccgctaaaa 20521 ttcggactca aatatatttt ttttatacga aaatatatat taaaacaaat atttttcata 20581 aatatttttg gtagttttag tgcagttatt gcagttccgt ttctgacaaa gttatgtaaa 20641 tttatccgtc gaagtatctg tccccgcaat ttttatctaa atatacatgc caaatattca 20701 aataaaaacg gagagtatta ttatttttta ataatacaaa aaatttttat aaaggggaga 20761 aaaggatgaa aaaagtcata tcaggtgcag tcgttattgc actgcttttt gcaataatga 20821 tacctgcgcg ttactttgga gcttttgccg aggcgagcca gacccttttt attaatgagg 20881 tcatgtcgtc aaacgtgtcc acaattcggg acggagatgt tacagaccct aaacatggaa 20941 gtaagggcgg agcatattcg gactggattg aaatctataa tgccggccct tatgatgtgg 21001 atttgacagg atacattctt gccgactctt cggcagagtg ggtattcccg caggggattg 21061 tgccggcagg agggtatctt ttggtttggg cgtctgacaa gaatatggtg gctcaagacg 21121 gccagttgca cacaaacttc aagctaagtg catccggcga gaacataaca ttaaaaaagc 21181 ctgacggaac gattgttgat tcagttgata ttatcggcct tggggatgac cagagctatg 21241 gaagaaaaag tgacggtgcg tcagagtttg ttgttttcat aaatccaacc cccggtgccg 21301 caaatgttta taacgctcct ggtacatcca ccctctttat caatgaggtt atggcttcca 21361 atacacgtac tataagggac ggagatgtgg atgaccctaa agacggaagc aagggcggag 21421 catattcgga ttggattgaa atttataatg ccggccctta tgatgtggac ttgacaggat 21481 acattcttgc cgactcttcg gcagagtggg tattcccgca ggggattgtg ccggcaggag 21541 ggtacctctt ggtttgggca tctgacaaga acaaggttgc tactgacggc gggctgcata 21601 ccaatttcaa aataagctct tccggagaag cattgacctt aaaggaccct gacggcaatg 21661 ttattgatat tttggtgact attaatcttt tggatgacca agcctatgga agaaaaaccg 21721 acggttcctc tgaactggtg ttgttaaaac ctacacccgg aactgcaaat atttatgatc 21781 caagtcttat acctgtttca gaacctgttt tttctcatca gggaggtttt tataccgggg 21841 cgtttaagct ggagcttacc acaaatgagc ccggagttaa gatttactat accacggacg 21901 gttctgaccc tgtgccgggg aaatcgggaa ccattgagta tacttccggc attaatataa 21961 agagcaggaa aggagaagcg aatgtacttt ccatgattca ggatatatcc aacgaccagt 22021 ggaacagatg gagagcacca aacggggaag ttttcaaatg taccaccatc aaggccgttg 22081 ccataagaga cgacggggca cggagtaaag tagtaaccca ctcatatttt gtagatcctc 22141 aaatgaatac cagatataca ctgcccgtaa tttctattgt aaccgactat gacaacttct 22201 ttgacaagtc taccgggatt tatgtaaatg ggaattatga gaacagggga aaagagtggg 22261 aaagaccggt acatatcgag tactttgaaa cagacgggaa acttggattc tccatggata 22321 tgggacttag gatacatggg ggatacacaa gaaagtatcc tcagaagtct ttccgtttgt 22381 atgctgacca caataatgat attggcgaga tcaaatatga gattttcccg ggacttagag 22441 gaacgggaac aggtaaaaaa ataaagagtt ttgaacgcct gattttgagg aatgcgggaa 22501 atgactggac aggtgctctt ttcagggatg aaatgatgca aagtctcgtt tcccacctga 22561 aaatagacac ccaggctttc agaccgtgta ttgtattcct gaatggagag tattggggaa 22621 tatatcatat tcgtgaacgt tatgacgata aatatcttaa atcacattat ggccttgatg 22681 atgacaaagt tgcaatactt gacgtttacc agactcccga agtccaggaa ggcgattcct 22741 cggatgtcct ggcatataca aatgatgtaa taaattattt aaaaacccat tccataactg 22801 aaaaaagcac atatgattat attaaaacaa agatcgacat agaaaactat atcgattatt 22861 atgtagctca gatattcttc ggaaatacgg actggcccgg aaacaatgta agcatatgga 22921 gatacaagac ggatgacggc caatatcatc cggaagctcc ttacggtcag gacggaagat 22981 ggaggtggat gctgaaagat actgattttg gctttggttt gtacggaaaa agtccgtcac 23041 acaacaccct tgcttttgca gccggtgata tacgcgaagg tcaagccaat gaggagtggg 23101 cggtatttct tttcaagact cttcttaaaa atgaagagtt caggaatgag tttataaacc 23161 gttttgccga ccagttgaat acttcgtttg taccgtcaag agtgatttca attattgatg 23221 atattgttgc aactttggaa ccggagatga aagagcatac ggaccgttgg ccgtttatta 23281 aattgacagc caccagcccg tgggatacaa cctggagcca ggaagtaaac agaatcagaa 23341 attatgccaa cagtcgtccg tcatatgtaa ggcagcatat attgagcaag ttccgcaata 23401 atggtgtaac gggtactgct cttgttactt tgaacactga ttcgacccgg ggacacataa 23461 ggattaattc cattgacata gtatcagata ctccggcagt tacaaatcct aatcgctgga 23521 gcggtactta cttcaaggga gttcccataa cgctaaaggc gataccaaaa gaaggctatg 23581 tgtttgacca ctgggagggt ataaacggat ccgttgaggc atcatcggat acgataacag 23641 tcaacctttc gaatgatttg aacgttacag cggtattcag gcctgaaaat gaaactcccg 23701 atcctgaaat tttgtatggt gactataatg gggatggagc ggttaactcc acagacttgt 23761 tggcatgtaa aaggtatctg ctttatgctt tgaaaccgga gcagataaat gttattgcag 23821 gggatcttga cggcaatgga aagattaact cgactgacta tgcatatctt aagagatatt 23881 tgttaaagca gatagataag ttcccggtac agttgaagta atactgatct tttgttaata 23941 tatatatata tatatataaa ttaagggggg aaatggaaaa gttggcaatt gaggtattca 24001 acaggtatga gaaaaagtat ttgatggaca acgatacgta tgagaaaatt aaagatattc 24061 tcgcagaata tatggagctt gatgaataca ataaagtgaa taatacatat tacacaatat 24121 gcaacatcta ctatgatact caagacaatc aattgataag acattcggtt tcaaaaccta 24181 gatataaaga aaaattaagg cttcgttcct atggagtgcc ttctttggat gccaaggtat 24241 atttggaaat aaagaaaaag gtaaacggac ttgtaaacaa gagaaggacg aagctgacat 24301 tgagagaggc ttatgaattt gctgcgacag gtataaagcc caaatatcaa agttacatga 24361 acaagcaggt tttgaatgaa atcgaataca ttttgagtgt gtatgacctt gagccaaagc 24421 tttatctggc atatgacaga atagcatatt ttggcataaa caacagagac ttaagaataa 24481 cttttgatac aaatataaga tcgcgaagga ctgatttgag gcttgaactg ggagactacg 24541 gtgaaccgtt gctggatgaa ggtgtttggc ttatggaggt caaagcggaa aagagcatac 24601 ctgtctggct ttcaaggatg ctgagcgaaa acggactctt taagaccagc ttctcaaagt 24661 atgggactga gtataagaaa atgattttga acaatattgc gaaggagaat gataacgatt 24721 gctcgacgga atatttgatg tcacggctgg cataaagctg tcttttggaa acatgacact 24781 ggcgctggca gtctcatttg ctttgggaat aattataagt ctggtatata ttaagactca 24841 tttgaaagag cattattctc agaactttgc tttggctttg gcgataatac cgagcattat 24901 tgccatgatg attatgctga tagggagcaa tgtggcgaga gcctttagct tggcgggagc 24961 ttttgcgatt attcgcttta gaagtgaaga agccactttt aaggatattg cttatgtact 25021 ttttgcaatg ggagcaggtt tggcctgtgg cgtcggattt ttgggctatg cgacactttt 25081 taccctggtg ctttgcggtt tcttgattgt tcttggcaaa gtaaatttcg gcgccaaaaa 25141 atcaccatat aaagtactga aggtcacggt tcgggatgat tttgactata agagggagtt 25201 tgaagaagtt tttaacaagt atgcattgag ctatgagcta aaaagagtca aggcaaagga 25261 tgaagagggc ctttacgtgc ttgagtatct tgtttctctt tattacgatg tcgatgaaaa 25321 agaatttatt gatgcgcttc ggtatcgcaa cggcaattta aatattgctc tgggtatgaa 25381 tgtcgaggga tagttgaaac aggtcaggaa gagaggttct tcctgacttg ttctgtattt 25441 atatgtttac aagatatttt ttttccaact ttataggttg ataagctgac ttggctctgc 25501 gcaacccttc cagacccaag tcttcctgaa gatttataaa ttccacatcg cgaaagaaga 25561 tttccaccaa agatttgttt agaaacggat aaatgccgga aaaattgtaa tttccttttt 25621 caaagtgaac tgttgccatc ttgctgttta atttttcgcc tattgcaaac cctgctattt 25681 cgttatttac ataaacagca atacctttta tattgttaaa gattttgata ttgctaaaaa 25741 tatcgaaaat ggctttttgc tcgtttttaa gctgttcact ttgcagggat ttataatcat 25801 accatttaag agacaaatca atacaatttt ttataacttc aggactttgt atttcttcaa 25861 ttctataatc gtatttcttt atgaattgat tgtactgatt cttttttctg tgatattttt 25921 tgcccgacag tttaatcaaa tccttgctgt tgaaaatata atcaaaattg tttttgtcct 25981 catgggatgt tacgagattt ccgaaatttt catgaagctg ctctaaaaaa ggtgtttcca 26041 cgtcaccgta gaggtatttg aagtcgggat tgttttttct taaggtgttg agctttaatg 26101 taatgtcggc aattttactt ttatcggccc caatgggctg catgaaatag gtgccgttgt 26161 tggcggtgcg ctttttaatg actattgcgt catcaactat tgcaaactct gtattgtaca 26221 tttttctcca tatataaagt gttaaaaatg aatattcata agtgagaaaa tcataatctt 26281 ttaaatattc atgaaaaagt tctctgtctt ttagctctat aggtttgaaa tccagcattt 26341 gtctcatcct acttttgtta tttctttatt tgtcctgatt aaaatgtctt aaatgttatt 26401 atacataatt tgcagctttt tggctactat ataaaagttg tatttaacga ataaaattaa 26461 tcatatggct ggttttatat tattatttta taaaattatt gtcttattta atgaatattt 26521 taattctaaa gtatatgccg cagctaattg taaataagtt gatatagtat ttattttctt 26581 attaatatat agcaattgct tgtatatcca taaattgtaa atgtcaataa aaatttggtt 26641 ctttccaaag ttggttgagt acactcattt ataaaaaaga taagaggcgt aaacccttaa 26701 atttcaaggt ttagcgtctc ttttacgttc tataactgtc aaactcggga tttttatttg 26761 ttatattgta ttttttcatt tattttaact aaccttggaa agaaccaatt ttatcttgta 26821 aaaacatctt agaaaaatat ggaacacaaa aagttaaagt aacagggtga aattcccatg 26881 gattaatctg tatgcatatg taaatttttt acttccaata aagactgctt tatgataaaa 26941 tgttgccgtc atatttttat tacaatttat taaaggtggt gtatcatgag taatgaagga 27001 aggtgtaaaa caggaaattt taaagccgta ttggccgtac ttatcgtaat ttttgtaact 27061 ctttcaggca gctttaaagc aagtgcaatt aacaagcaga ttttcattac tgtaaaggta 27121 aacggcaact atattaaaat ggacacgtgg ccgtatatta aagacggcag gacatttgta 27181 tcagtacgct ttgtcgctga agctctcgga gcaaaagtgg aatgggatgg cgaagacaag 27241 aaagttacaa taaaagatga ctacaattat attgaattgt ttgtcggaag caaagaatgt 27301 tatattaacg cagtgaagca agcaatggat acctctcctg aaatatcaaa cggacgcaca 27361 atggtgccat taaggttcgt atctgaagtt ctcggatgtt cagtcgattg gaatcagaca 27421 acatatacag tctcaataaa caaaaacggc attaaagttc ccgaagaatg tatataccaa 27481 agaaattata ccgatgagga tttgatttgg cttgccagga tagtaactgt tgaggcaggg 27541 ggaatgtctc ttgagggaaa acttgcagtt gcaaatgtcg tattaaacag agtaaaaagt 27601 ccttcatttc ccaataccgt gtatgatgtt atatttcaaa aaggccaatt tcctccggca 27661 tacaagagcg gctttaaaga gttggtgccc ccggacgagt gtattattgc ggcaaaaatg 27721 gctttggagg gtgtcaacaa cattgggaac tgtctgtatt tcaataacag gccgtttact 27781 tcaagaagcg gtacttttta taaaaaaata gaaggtgagt acttctatta ctaagtcaaa 27841 aatatgataa aatcaaattg ccgaaaataa aaaagtatga aatagaagat atataaaaga 27901 tatataaaaa atatataaaa acagccaaaa aagcagatga aaagataaaa acagataaaa 27961 acaggtactt gtcaaaaagg tacctgtttt ctttatgaaa atttatatat tcttgattct 28021 cattaccctc atggcattga gaactgccaa cagcgcaact ccgacatctg cgaaaacggc 28081 ttcccacatt gtggctatgc ctgctgcgcc gagcagcaag accaggactt ttacgcccaa 28141 cgaaaaaata atgttctgcc agataatggt tttggttctt ttggcaattt taatggcagt 28201 gacaagcttt gagggctcat cggtcatcag caccacatct gcggcttcaa ttgcggcatc 28261 cgagccaagg cctcccattg cgacacctat atccgaccgc gccagcacag gcgcgtcatt 28321 cagcccgtca ccgacgaata ccagtttgcc accttttgat ttgctgccat ccaaaagttc 28381 aaccacttcc accttttgat gaggcaaaag ttccgcatgc acttcatcga tttccaattc 28441 gcttgcgacc ttgcgggcag tgctgttatt gtctcccgta agcattacca attttcttac 28501 accgatttct ttcagttctt tcaaagcttt ctttgagtcc gcctttattt catccgaaat 28561 aacaatgttg cctgcgtaga ttccgtcaac ggccacatgg accacagttc ctgaagtttc 28621 aatattggca aaggatatgg ccgctttggc cataagcttg gtatttccag caagtatctc 28681 tttgccatgg tagacggctt ttattccatg accggcaatt tcttcatagc tttccacttt 28741 acttttatca atagttttgc cgaaagcttt cgttattgac aatgcgattg ggtggtttga 28801 aaagctttcc gcataggctg catattttag taaatcttcc tttgtaaaat ccgatgaagg 28861 atttacctcg gttacattaa acacaccttt tgtaagggta ccggtcttat cgaacactac 28921 ggtatcaaca ctgtttaatg cctcaagata gttgctgccc ttaatgagaa tgccgttttt 28981 tgaagctcca ccaattccgc cgaagaatcc aaggggtatg gatattacca gagcgcaggg 29041 acatgagatt acaaggaata tgagtgctct gtaaacccaa tcgaagaaac cggcaccggg 29101 aacaaccagc ggaggaatta ctgccaaagc cgctgagata aaaactactg cgggagtgta 29161 gtatcctgca aactttgtga taaagttttc ggtcggagct tttctgctgc ttgcgttttg 29221 caccaagtcc agtatttttg acaccgtgga ttcgccaaac agttttgtaa cttctacact 29281 gataaggctc gtttgattga ctgtaccgga gaggacagct tcaccggggt aaacttcccg 29341 cggaaccgat tcaccggtga gggccgatgt atccagcatt gttcttcctt caacgacttt 29401 accgtccaaa ggtattcttt cacccggctt tacaactatt atgtctccga ctttaatatc 29461 ctccacggat acttttttta tgctgttgcc gactttgaga ttggcatagt cgggacgaat 29521 atccataaga gcggcgatgg actttcttga acggtttacc gccatattct gcaagtattc 29581 acctatttgg taaaaaatca taaccgctac gccttcagga aattctccga ttaaaaatgc 29641 tcctattgtc gctatgccca tcagaaagtt ttcatcaaat actttaccct ttgatatatt 29701 acggaaggct tttagcaaca cttctcctcc gataattata tagcttgcaa agaacaggcc 29761 gaattcaacc cagtagttta aattcaggga cagtgcggcg acaaataaaa gtattccgac 29821 accgagtttt acaagctctt ttttgtcgaa gtcattatcg ttggatttca atttttcatt 29881 ggtcttatcc tgtttctcga tgacttcaat atcctcttct atgcttttgg caatcaaaga 29941 cgcctgcttt ataatatttg ccggttcgag cgtgtcatcc agttccagga tcattttttt 30001 tgacacaaaa ttaaccgagg cgttttttac tccttcaatt tctcttacct ttgtttctat 30061 tttttgtgca cagttggcac aattcaaacc ttcaagtatt aattctttct taattgcctg 30121 tttcatttta cacctgcttt ccggattcat tttccgttat atgcatatag ccttggtcaa 30181 atatttgttt tatatggtcg tcatccaaag agtagtacac gactttgcct tcttttctgt 30241 actttacaag tcttgcctgc tttaatactc tcagctggtg ggaaactgcc gactgagtca 30301 tgtttataag tgcggcaata tcgcacacac acatttcaga ggcaaacaat gcgtatagta 30361 tttttatcct ggtggtatct ccaaaaactt tgaaaagctc tgccagatca taaaggtgct 30421 cttctttggg catgtttttt ttcactttgt ttactatgtc ttcgtgtatt acattacatt 30481 cacatgaatc tcgtttttca tccaaaacta attcctcccc tgcatagttg ataaattgaa 30541 catatgaata tgtattcata tattacactt ttattatatg ccaactgaat aatggtgtca 30601 atattttatg tatcaaaaaa ctattaatga aacaaaaaaa ttttataaga aaaaagttga 30661 taataaaaga aaaacctacc cctaaaaaaa ttagaatttc gtatttataa aaagagggac 30721 atatcagtga atttttaaaa gggaggatga aagatgaaaa aaactcttgt atttttaacg 30781 gccttgagtc tgatattcac gctgtttatc agttattccc tgtcagcagg accggcttca 30841 accaagtatg gggatctcaa tgccgatggc aagatcaatt cgacagatta caacttgggc 30901 aagagattga ttctgagaac aatttcggag cttcccattt ccaatggatc tgtagccttt 30961 gaccttaacg gtgattcaaa ggttgattca acggacctta ctgcgctgaa aagatacctg 31021 ctgggtgtta ttgacaagtt tccggtgggc acggatatac catcccaaac acaaaagacg 31081 agatatcagg ctgaggatgc gatgttgtac aaggcattcg aggaaacaat ccatgcaggt 31141 tatgacggga gaagttatgt aaattacgac aacgaacccg gaggatatat tgagtggaat 31201 gtaaatgtat ccagttcagg tacatataag cttattttca gatatgcaaa cggatcaaac 31261 aataacagac ctatggaaat aagagtaaat tccaatctgg ttgcaggtag tctggacttt 31321 tatccgactt cagcctggac tgtatggaat gaccaaagca tagttgtaac tttaaatgcg 31381 ggcaacaacg ttatcagggc aacgggaatt gcctcggacg gcggaccgaa tgtggattat 31441 cttgaagtaa ttccgacaaa tgaaccacca gcacccaccc cttcaccgac gcctacagtt 31501 ggacctacac ctactggtgc gcgtcagatg gagagactgg acagagggct tgtggcggta 31561 aaagtaaaca acggagtatt tttaagctgg agaatgtttg gtacggatcc ttccaacatt 31621 gcattcaact tgtaccgcaa cggaacaaag ataaattcca caccgattac cggtgcgaca 31681 aactatgtgg ataccggcgg aacgacaagt tcaacataca cggtacgtgc ggttattaac 31741 ggacaggaac aggaggcatc aaaacctgta agtgtctggg ctcagaatta tcttcagatt 31801 cccattcagc caccgtcaag cgcgtacgag gctaatgact gcagtgccgc agaccttgac 31861 ggagacggag aatatgaaat tgtgttaaag tgggagccaa ataacgcaaa agacaattcc 31921 caatccggat ataccgataa tgtgtatttg gatgcttaca agctgaacgg cacacgtttg 31981 tggagaatag atcttggaag aaatatccgt gccggtgccc actataccca gtttatggtt 32041 tatgaccttg acggcgacgg caaggcagag gttgcatgca agacagctga cggaacaaga 32101 gacggaaaag gaaatgtgat aggcaatcca aatgcggatt atcgtaattc aagcggatac 32161 atactttcag gacctgaata cctgacagta ttcgatggac agacaggtgc cgccattaca 32221 acggtggatt atgatcctcc gagaggaaat gtctcttcat ggggtgacaa ttacggaaac 32281 agagtggacc gtttcctggc gtgcatagca taccttgacg gtcaaagacc aagccttgtc 32341 atgtgccgcg gatattatac aagaagcgtg cttgtggcct gggatttcag aaacggaagg 32401 cttacaaaga gatgggtatt tgacggcaac aattacagcg gatataacgg acagggtaat 32461 cacaacctga gtgtggccga tgttgacggc gacggaagag atgagattat ttacggtgca 32521 tgtaccattg atgacaacgg aagaggattg tatacttcag gacttggcca tggggacgct 32581 ctgcatgtgg gagatcttaa tcccaacaga ccgggccttg aaatttggag ctgctttgaa 32641 agctccggcg gcgctgcttt gcgtgatgca aggacaggag aagtgttgtt cagatggcat 32701 agatccagtg atacaggaag ggcttgtgcg gctgatataa cggcatcatc tccgggagct 32761 gagctttggg ctgcaggttc tccgctgttc agctgtaccg gtcagaatat aggaactgct 32821 ccaagccaga ttaactttgc tatatggtgg gacggagacg aactcaggga gctccttgac 32881 ggcattacaa taagcaaata cggtgtagga acattgttta ccgcgaccgg atgtgcttcc 32941 aacaacggta caaaatcaac tccgtgcctc caggcagacc tccttggaga ctggagagaa 33001 gaagtaatct ttagaacttc ggacaacagg tatttgagaa tatacaccac aacggcaaca 33061 acaaacagac gtatttacac attaatgcat gatccggttt acagattggg tatagcctgg 33121 cagaatgtag catacaatca gccgccgcac acaagcttct ttatcggagc cggcatggct 33181 gagcctccga agccaaatat ttaccttgtg ccgtaatagt gcttgaaatg ggctaataga 33241 ggattgaaaa aggaaaataa ataatggagc tgttgcacat caaatgggat gaatttcact 33301 gcaacggctc tttttttatg ggaaaacttt tgaaaaaatt acggatatgt tgtaatatta 33361 ttaaaacatg ctattaaaat atactacatt agatatacta atgaaatata ccgttaaagt 33421 ggcaatatac tataaaaaca acttatttta gtaaacagat tatattaaaa catcagggtt 33481 ggcgggagga acaatgaata caaagcaaat gtatttaaag cgtatgcgcc aatatagtga 33541 taatgcggaa aagcttgaaa gacgttcggg aatgtacagc acaataaggc ttgtaacatt 33601 tgcagcaggt acattcttta cggttcttgc ttttatgtat ttaagcaatg tccatgggtt 33661 tattagtatg ggagtatttt ttgtactgtt tctctttttg gttgcgaagc atcaaaaagt 33721 aatcgatgaa actttaaaat atcgcactct tgcggaaata aacaaaagat gcgtagcaag 33781 aatggaaggc acgtggacag agtttgagga caagggagag gaatatgcag acccaaatca 33841 tatgtattcc aaagaccttg atgtttttgg gcatggttct ctttttcagt ggatcaatac 33901 gacaaataca ttttttggca gggaaaggct aaggcgtttg ctggaattcc ctgaaaagga 33961 ggccggacaa atcagaaaga ggcagaatgc ggtaaaggag ctttcaaaga aaattgactt 34021 ttgccaaagt ttgcaatgtg aaggaatgat ggctcaaaat gcttcgaaaa atcctgacaa 34081 gctacttgac ttttgtgagg acggcaccaa actttttcgt aacaagcttg cagaacagtt 34141 gttttatatt cttccggagc ttacaatagt ttttctggtt atatgctggc ttgattcttc 34201 ggtttcgctg tacatacctt tctttcttct tggtgtgcag gctgtcataa atattgtgtt 34261 gcatggcagg gtaagcagta ttttaggtcc ggtaagcagg tacaagaatg agataaaggt 34321 attttataaa atgattgaac tgattgagaa gaaagagttt gaggatgaat acctgataga 34381 attaaaatcc cggctgtttg acaagaagaa accggcatcg gagcagataa aggatttgga 34441 gaaaattgta gaggccactg acatcgggaa gggttatata gttgagattt tattgaattt 34501 tttcttgttc tggaatattc actgtgtgtt tgcattggaa agatggaaag caaaggccgg 34561 taaggcaata cgaatctggc ttgagactat aggtgatttt gaagctttgg caagtttggc 34621 tcttgttgcg cagatgaacc ccgaatgggc tttccctgaa atatcggacc ggaaggtttg 34681 ctttaatgcc gttgacatgg gacatccttt gataaatgag ggtaaacgtg tgtgcaacag 34741 tatcaatatg gacaataaaa tctgtattgt tacagggtcg aatatgtccg ggaaaaccac 34801 gcttttgagg actgtggggg taaatcttct gcttgcttat gcgggaaccg ccgtttgtgc 34861 caaaaaaatg acatgctccg taatggatat atgcacttca atgagggttg tagatgattt 34921 gaacgaggga atatccactt tttatgcgga gcttttaaga ataaagatga taatagacca 34981 ctcaagaatg aaaaagccga tgatattcct tattgacgaa gtgttccggg gaaccaattc 35041 ccttgacagg gtcacaggtg cacgaaatgt tttgctgaat cttgacaaag actgggttat 35101 cggaatgatt tcaacccatg attttgaact ctgcaatttg gaaaaaggac gcgaaggaag 35161 gattgtcaac taccactttg cagaaaccta caccaacaac gaaataaaat ttgattatat 35221 tttaaggcgg ggacagtgta aaaaaagcaa tgcaagatat ttgatgaaaa tggtgggaat 35281 agagcttttg gatgagtgac aaatcttggt ttgttgaccc tgaaaaaact ggatgctata 35341 attttaa //