LOCUS NZ_ACVX01000041 27383 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00048, whole genome shotgun sequence. ACCESSION NZ_ACVX01000041 NZ_ACVX00000000 VERSION NZ_ACVX01000041.1 GI:256005780 DBLINK Project: 55637 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. REFERENCE 1 (bases 1 to 27383) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Hemme,C.L. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The draft genome of Clostridium thermocellum DSM 2360 JOURNAL Unpublished REFERENCE 2 (bases 1 to 27383) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Hemme,C.L. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (17-JUL-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000041. URL -- http://www.jgi.doe.gov JGI Project ID: 4085028 Source DNA and Bacteria available from Christopher L. Hemme (hemmecl@ou.edu) Contacts: Christopher L. Hemme (hemmecl@ou.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Annotation by JGI-ORNL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Clostridium thermocellum DSM 2360 Culture Collection ID :: DSM 2360 GOLD Stamp ID :: Gi02594 Isolation Site :: contaminant of Clostridium thermocellum strain LQ8 Isolation Country :: USA Oxygen Requirement :: Anaerobe Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Sporulating Temperature Range :: Thermophile Temperature Optimum :: 60 C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Phenotypes :: Cellulose degrader, Ethanogenic, Ethanol production Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Location/Qualifiers source 1..27383 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene complement(56..829) /locus_tag="ClothDRAFT_2594" CDS complement(56..829) /locus_tag="ClothDRAFT_2594" /inference="protein motif:PFAM:PF00106" /note="PFAM: short-chain dehydrogenase/reductase SDR; KR domain protein; KEGG: dal:Dalk_0259 short-chain dehydrogenase/reductase SDR" /codon_start=1 /transl_table=11 /product="short-chain dehydrogenase/reductase SDR" /protein_id="ZP_05430733.1" /db_xref="GI:256005781" /db_xref="InterPro:IPR002198" /db_xref="InterPro:IPR002347" /db_xref="InterPro:IPR013968" /translation="MQKRDLFSLKGRVAVVTGASSGLGVQMAKALAAQGADIVILARR KEKLEKVAEEIRQFGVRCLPIECDVTKIEMVRKAAELAEKEFGKVDILINNAGSGGIA SAEETSDEMWSNTINVDLSGVFMVAREFGKIMIKNKYGRIINISSIYGMVGNMAHPSA AYHAAKGGVINLTRALAAEWAKYGITVNAICPGYFVTELTEDVLNTEYFTNYMKSTVP MGRYGKEGELDSTAVYLASDASSYVTGAVIPVDGGYTCV" misc_feature complement(59..829) /locus_tag="ClothDRAFT_2594" /note="gluconate 5-dehydrogenase; Provisional; Region: PRK08213" /db_xref="CDD:181295" sig_peptide complement(731..829) /locus_tag="ClothDRAFT_2594" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.993) with cleavage site probability 0.954 at residue 33" misc_feature complement(<512..808) /locus_tag="ClothDRAFT_2594" /note="Rossmann-fold NAD(P)(+)-binding proteins; Region: NADB_Rossmann; cl09931" /db_xref="CDD:186874" misc_feature complement(order(539..544,701..706,761..769)) /locus_tag="ClothDRAFT_2594" /note="NAD(P) binding pocket; other site" /db_xref="CDD:133449" gene complement(984..1574) /locus_tag="ClothDRAFT_2595" CDS complement(984..1574) /locus_tag="ClothDRAFT_2595" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430734.1" /db_xref="GI:256005782" /translation="MEKFYVYVVLTRTNTVISRLIQLFKKDEFTHAAISLDRDLANMY SFGRKYTFNPFIGVFKHENLNKGTYKYCKVLPGAVIELEVTKQQYQRAKALLQCFISN AGRYKYNYMGLVNGLLNREACHDSRFVCSEFVYYILNESGIADFKISRNLVRPQSLLK LNGRIIFKGDLKNTRLLKKCLRSHKIHVNQKLSVAL" gene complement(1744..2325) /locus_tag="ClothDRAFT_2596" CDS complement(1744..2325) /locus_tag="ClothDRAFT_2596" /inference="protein motif:PFAM:PF00300" /note="PFAM: Phosphoglycerate mutase; KEGG: rme:Rmet_0420 phosphoglycerate mutase" /codon_start=1 /transl_table=11 /product="Phosphoglycerate mutase" /protein_id="ZP_05430735.1" /db_xref="GI:256005783" /db_xref="InterPro:IPR013078" /translation="MTNVYFVRHAEPDYNVRDDFARPLTKKGKEDAKLVSSYLSDKDI HVVISSPYLRAIDTVKDFAGEVGLDIKIVDDFRERKVGNVWIEDFNSFAKKQWEDFDY KLSDGESLREVQNRNIKALNEVLRMYEGKNIVIGTHGTALSTIINYYDSTYGYDDFER MKLIMPWIVKMQVKKDRCINITKIDILNGGRSL" misc_feature complement(1828..2319) /locus_tag="ClothDRAFT_2596" /note="Histidine phosphatase domain found in phosphoglycerate mutases and related proteins, mostly phosphatases; contains a His residue which is phosphorylated during the reaction; Region: HP_PGM_like; cd07067" /db_xref="CDD:132718" misc_feature complement(order(1909..1914,2164..2166,2299..2304)) /locus_tag="ClothDRAFT_2596" /note="catalytic core; other site" /db_xref="CDD:132718" gene complement(3107..4009) /locus_tag="ClothDRAFT_2597" CDS complement(3107..4009) /locus_tag="ClothDRAFT_2597" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430736.1" /db_xref="GI:256005784" /translation="MKDKNLDFLKKQYLHTPIPEELDSVVKKALKDGSEHRMKRNKLI KRTSVAAVLIVVFAGVLTVGINSSPAFASALEKVPIVGGIVKVLTFKEYTVNEDRFKA DIKVPAIEGLENKELQNSLNEKYLAENKKLYEEFMAEMENMKKEEGGYLGVDSGYVVK TDTDRILSIGRYVLTVAGSSSTVFKYDTVDKKEGILITLPSLFKDNSYIEIISENIKD QMRERMKADDGFIYWVDVDENEEFIEPFNKISPNQNFYITEEGKLVISFDKYEVGPGY MGIQEFEIPTEVIADILVSNEYIR" misc_feature complement(3131..3469) /locus_tag="ClothDRAFT_2597" /note="Protein of unknown function (DUF3298); Region: DUF3298; pfam11738" /db_xref="CDD:152174" gene complement(4011..4514) /locus_tag="ClothDRAFT_2598" CDS complement(4011..4514) /locus_tag="ClothDRAFT_2598" /inference="protein motif:TFAM:TIGR02937" /note="TIGRFAM: RNA polymerase sigma factor, sigma-70 family; PFAM: Sigma-70 region 4 type 2; sigma-70 region 2 domain protein; sigma-70 region 4 domain protein; KEGG: mlo:mll8140 RNA polymerase sigma factor" /codon_start=1 /transl_table=11 /product="RNA polymerase, sigma-24 subunit, ECF subfamily" /protein_id="ZP_05430737.1" /db_xref="GI:256005785" /db_xref="InterPro:IPR007627" /db_xref="InterPro:IPR007630" /db_xref="InterPro:IPR013249" /db_xref="InterPro:IPR014284" /translation="MLLKNKYLEEQIAEYAVKHKENFYRLAYSYVKNADDALDIVQES VYKAISSMDSLKNPDYLKTWFYRIVVNTSLDFLRRQKRNVAVGEEILGSFDFGAVDSY GDFDLQRALDNLPEKYRTIVVLRYFEDLKIDEIAEILNENVNTVKTRLYKSLEILRVK LSECEEV" misc_feature complement(4032..4487) /locus_tag="ClothDRAFT_2598" /note="RNA polymerase sigma factor, sigma-70 family; Region: sigma70-ECF; TIGR02937" /db_xref="CDD:163078" misc_feature complement(4269..4445) /locus_tag="ClothDRAFT_2598" /note="Sigma-70 region 2; Region: Sigma70_r2; pfam04542" /db_xref="CDD:146937" misc_feature complement(4041..4199) /locus_tag="ClothDRAFT_2598" /note="Sigma70, region (SR) 4 refers to the most C-terminal of four conserved domains found in Escherichia coli (Ec) sigma70, the main housekeeping sigma, and related sigma-factors (SFs). A SF is a dissociable subunit of RNA polymerase, it directs bacterial...; Region: Sigma70_r4; cd06171" /db_xref="CDD:100119" misc_feature complement(order(4053..4055,4059..4064,4068..4076, 4080..4085,4089..4091,4119..4124,4140..4142,4170..4172)) /locus_tag="ClothDRAFT_2598" /note="DNA binding residues" /db_xref="CDD:100119" gene complement(4721..5065) /locus_tag="ClothDRAFT_2599" CDS complement(4721..5065) /locus_tag="ClothDRAFT_2599" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430738.1" /db_xref="GI:256005786" /translation="MEEIVCDYDAFRQAAFEFRRNPTPENAQKVVDRYTGRYLDDMEA LWAESTRLSCEDIFLQAAETLLESYRESGERTKIMELLHRCTGLSCHSHRVDVVRVDA EKEGKKKKNKTL" gene complement(5189..5413) /locus_tag="ClothDRAFT_2600" CDS complement(5189..5413) /locus_tag="ClothDRAFT_2600" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430739.1" /db_xref="GI:256005787" /translation="MLKISFDMAENKCLHTAVTQPKKVYINTLGAFYIAPAHDRCSRV KIRTQKSRELLAYLLEHREDVSRERIFADL" gene complement(5394..6002) /locus_tag="ClothDRAFT_2601" CDS complement(5394..6002) /locus_tag="ClothDRAFT_2601" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430740.1" /db_xref="GI:256005788" /translation="MEDWYMVMIEKIYNLTLATRLSEALVLTKAMAEKCAVQGSSRVR AWLECYLTVIYFYQGDYIKCLQYYEKSLSLPEEEQDWLSRHCMGAYAAKTYQLTGQEE RSIPLMESEISRLRRLGLYDELSLNYLIYAEILLTEEIRKLSQGSFADFLAFRRYLEM AEEHAALNRSTEDYLLFAKILRLTSEVLIRPNKALHDVKDIL" gene complement(5981..6913) /locus_tag="ClothDRAFT_2602" CDS complement(5981..6913) /locus_tag="ClothDRAFT_2602" /inference="protein motif:COG:COG2909" /note="KEGG: pmy:Pmen_2539 regulatory protein, LuxR" /codon_start=1 /transl_table=11 /product="ATP-dependent transcriptional regulator protein-like protein" /protein_id="ZP_05430741.1" /db_xref="GI:256005789" /translation="MRYLIPKEVAEKWGIPVRRVQILCKQGKIKGAERHGRIWLIPET AEKPEDAFSLPKVPCAIVPRPRLTEKIAPPGSSLTYIHADAGYGKTTLMMQYAGERND VVWLSLDERDSNMTYFLRHLEDAFRKKFPRFDFYATDLLPFAARDTFVSRALSALLCA IGRRKLTLILDDVHVITNGATMDFLNRLVKSCPSSLTLVMAGRYELWSGLFRLKMEGR IVELTKADLCFSREEAQKLWGFFDEAAYTATEGWALVLQSYRVAAENGKLCLPQADRK LYSYLMEFPWRRAAVCSTAFCLCRRMLLWKIGTW" misc_feature complement(<6065..6760) /locus_tag="ClothDRAFT_2602" /note="ATP-dependent transcriptional regulator [Transcription]; Region: MalT; COG2909" /db_xref="CDD:32733" gene complement(7030..7953) /locus_tag="ClothDRAFT_2603" CDS complement(7030..7953) /locus_tag="ClothDRAFT_2603" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: scl:sce5147 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430742.1" /db_xref="GI:256005790" /translation="MLSSIEFIKQSLGLHLFFARIMKEHSFFLEVGFTSKDSGFIQQA DAFRREFDGILGETISLSNSVVNPEILQSGEVITPFTLNAEMATIYFTGVRIPTQLTQ AEARLAGSDIITANPVLERHVSVLNQRAINATAALIQFKKNILSNVLYCKMFTFNYPL LILHITREAELYLLQLRRLQRREEIDLAKEAYELQFFWNRQMAEHAKFIRGLLDPTEN DLINQANDFGNEFDQLTAEAKAAMDATSPMAKVTDESLKATEDFRNFKAQGTQAILEC KVKSIIIPLLGDHVLREANHYLRLLKMFEGR" misc_feature complement(7783..7914) /locus_tag="ClothDRAFT_2603" /note="Protein of unknown function (DUF2935); Region: DUF2935; pfam11155" /db_xref="CDD:151598" misc_feature complement(7243..7374) /locus_tag="ClothDRAFT_2603" /note="Protein of unknown function (DUF2935); Region: DUF2935; pfam11155" /db_xref="CDD:151598" gene complement(8336..8761) /locus_tag="ClothDRAFT_2604" CDS complement(8336..8761) /locus_tag="ClothDRAFT_2604" /inference="protein motif:PFAM:PF00583" /note="PFAM: GCN5-related N-acetyltransferase; KEGG: abu:Abu_1271 acetyltransferase" /codon_start=1 /transl_table=11 /product="GCN5-related N-acetyltransferase" /protein_id="ZP_05430743.1" /db_xref="GI:256005791" /db_xref="InterPro:IPR000182" /translation="MIHFPHKSIEEIHSFLEVVSAEWQKKEPSYYEFAIVYDSVHIGA VCVYLNEDRTEGELGWILNKKYWGRGFATEAALAIKSFAINQLKVKKLLARCDCRNLN SARLMEKIGLSFESKSERQYPDERGVAGELIYSCVVIDE" misc_feature complement(8429..8659) /locus_tag="ClothDRAFT_2604" /note="N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of an acyl group to a substrate; Region: NAT_SF; cl00443" /db_xref="CDD:185998" gene complement(8758..8868) /locus_tag="ClothDRAFT_2605" CDS complement(8758..8868) /locus_tag="ClothDRAFT_2605" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430744.1" /db_xref="GI:256005792" /translation="MYIELRNERLLLRPFSVLDLHTVYEYASYAENTKYI" gene complement(9022..9267) /locus_tag="ClothDRAFT_2606" CDS complement(9022..9267) /locus_tag="ClothDRAFT_2606" /inference="similar to AA sequence:KEGG:KPK_4970" /note="KEGG: kpe:KPK_4970 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430745.1" /db_xref="GI:256005793" /translation="MKTCIACGMPMKDISDFAANDPNKDYCVYCSRPDGEMQSFEEKK ESLTKFIVKTQGADENVAWKIAENMMHKLPAWKEYFN" gene 9901..12438 /locus_tag="ClothDRAFT_2607" CDS 9901..12438 /locus_tag="ClothDRAFT_2607" /inference="protein motif:PFAM:PF00089" /note="PFAM: peptidase S1 and S6 chymotrypsin/Hap; KEGG: met:M446_5887 protease Do" /codon_start=1 /transl_table=11 /product="peptidase S1 and S6 chymotrypsin/Hap" /protein_id="ZP_05430746.1" /db_xref="GI:256005794" /db_xref="InterPro:IPR001254" /db_xref="InterPro:IPR001940" /db_xref="InterPro:IPR008256" /translation="MIYFCHAPNDRAIAEKIYFSLKQTGLTCWIPSQDIMAGQYYVEA IANAIEKSDIVVFIFSSHSNTSIQVIDELQKASSLNKTIIPFCVDRAMPSEPIEHYLS SPYKVDATIGLPDDNIAKLQNIIKQIYNQSSVHNANDINTVKDTFAPSNPLENNTNII KDASDNNSQESIQFNGVINSANSQPPQNPNLPPQHQMNPNFNNTVNNMDRNMNYPIKQ NVRPNPQVKSSNKIKIPLIIGGSIAGGIILLSILFMLGKNLLFSTLINSHRPSDNPVI GSNIRIDGSKYTEEEIIEFTKNAILELDRLQASREFYDTYAYNAPEYDLMRIQENVFF SLMDLDVVRFEEVAILGKNEKEHGIEYLLKVDFVCYADYEYTENNETVHRKGEALLYN NVVILETPNDGLKYLYMEGIFEDELNARNENLESSYREIAESGDVNTEDTIQTYLPDS TDEDTIMSVKDIVKKNDHKVVAVYVDVPGGQSQGSGFFIKDGVIVTNYHVIEGGKSAK ILLSNGNYVDVEGVLYTDSDVDIAVLKLVNEVGIEPVTIGQARDSDKGSIAVAIGSPL GLFNTVSTGIISNFWEANGVNLIQISIPITHGNSGGALFNESGKLIGITSSGIGEANL NFAISSTHIIPICEDIKNIPYNQLNAVPLSSAGGNINSITRQAGSSNSNQSSSSGKSL LSSKYRFVNSDKPISNDATYTSDLQTIYDLALNKYYAKEDLYRDLDYAFDYILDYGTK YYLEYLEYILHETYIKEDVENFNKINESVLEVLNTTGEYIEFTADEIEIIGAGIKNDG TIDGIIVRALFYENSEPYVRSKFYFRANYDAWSYVDGFLYVGEVTEK" misc_feature <10000..>10128 /locus_tag="ClothDRAFT_2607" /note="TIR domain; Region: TIR; cl02060" /db_xref="CDD:186511" misc_feature 11317..11778 /locus_tag="ClothDRAFT_2607" /note="Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic...; Region: Tryp_SPc; cl00149" /db_xref="CDD:153545" gene complement(12783..13319) /locus_tag="ClothDRAFT_2608" CDS complement(12783..13319) /locus_tag="ClothDRAFT_2608" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430747.1" /db_xref="GI:256005795" /translation="MKIKRIVIILTVIILLAVFPILYYYKSSIGFNIERITGTQQGMI NKYFHTKLNDESLIKNFVIKKDIEFHYCIEAELAVPENKINEIFAYYDKHKKITVEEM DEDYFRAQMYSKIGVNKDNFDYQYNIFDSVYRKALLGVSETHTQRTSTLIFTKPVDGY VRVYLYIDKLGWKLADKT" gene complement(13664..15076) /locus_tag="ClothDRAFT_2609" CDS complement(13664..15076) /locus_tag="ClothDRAFT_2609" /inference="protein motif:TFAM:TIGR00797" /note="TIGRFAM: MATE efflux family protein; PFAM: multi antimicrobial extrusion protein MatE; KEGG: dal:Dalk_2553 MATE efflux family protein" /codon_start=1 /transl_table=11 /product="MATE efflux family protein" /protein_id="ZP_05430748.1" /db_xref="GI:256005796" /db_xref="InterPro:IPR002528" /translation="MKDISANTSSSGNPLGYAPVLGLIGKFAIPSIISMLVAAAYNIT DQIFIGRLVGMLGNGATSVAFPVVTFTTAFAQLIGVGTAANFNINMGAKREKEAKHFV GTGITLMPTVGLLIFCIVFVLKKQILLLCGATENVLPLAMTYLGITAIGLPFQLFTTA SSSLIRADGSPTYSMICNVTGAILNVFLDWLFMFVFGWGIQGAATATVIGQIVSFLLC AIYYFKFRTFQITRRMLGIKWYYAKRIAKLGTSNFINHTIMMLVNIVLNNSLKIYGAM SIYGSDIPLAVSGIIAKLNSVLSAFSIGLAQGCQPILGFNMGAKNYSRVKETYKKAVS IAIGISVLAFILFQCFPRQITGIFGSGDELYGDELYFEFAEKYLKIYMFMVCVFGIQP VTINYFTGTGNVRQGIILSLSRQGFFLIPLLLILPQFLGLTGVLYAGPIADFMACLLS LTMITLNFKKLDSGREVADK" misc_feature complement(13688..15001) /locus_tag="ClothDRAFT_2609" /note="multidrug efflux pump VmrA; Reviewed; Region: vmrA; PRK09575" /db_xref="CDD:181964" misc_feature complement(14504..14989) /locus_tag="ClothDRAFT_2609" /note="MatE; Region: MatE; pfam01554" /db_xref="CDD:144956" misc_feature complement(13835..14302) /locus_tag="ClothDRAFT_2609" /note="MatE; Region: MatE; pfam01554" /db_xref="CDD:144956" gene complement(15073..15531) /locus_tag="ClothDRAFT_2610" CDS complement(15073..15531) /locus_tag="ClothDRAFT_2610" /inference="protein motif:PFAM:PF01047" /note="PFAM: regulatory protein MarR; SMART: regulatory protein MarR; KEGG: dal:Dalk_1623 transcriptional regulator, MarR family" /codon_start=1 /transl_table=11 /product="transcriptional regulator, MarR family" /protein_id="ZP_05430749.1" /db_xref="GI:256005797" /db_xref="InterPro:IPR000835" /translation="MNKSIGRLINLLARKSQMYLGKVLSQYNLTAAEEPFFMALQNRE GITQEELTSIVCVDKAATARAVRSLEEKGYLLRIQDEKDKRQNRVYPTEKAKQIGQEV RTELMKFNKLLTRNIKEEDLDRLYSLLLQMEENMNKIFKEMIYQGNEEQE" misc_feature complement(15136..15501) /locus_tag="ClothDRAFT_2610" /note="Transcriptional regulators [Transcription]; Region: MarR; COG1846" /db_xref="CDD:32031" misc_feature complement(15166..15468) /locus_tag="ClothDRAFT_2610" /note="Winged helix-turn-helix (WHTH) DNA-binding domain of the GntR family of transcriptional regulators; Region: WHTH_GntR; cl00088" /db_xref="CDD:185792" gene complement(15834..16619) /locus_tag="ClothDRAFT_2611" CDS complement(15834..16619) /locus_tag="ClothDRAFT_2611" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430750.1" /db_xref="GI:256005798" /translation="MQNYYSNPWINYYENYTDNRFNYANRQNTYTIDRQIGDVNGDGV PDIVYLVGDKNQNNFYENIRLMIQDGSTLHWYVVPLYPDYSMAFDAWLFLGSFTGFSS KEIMVSLSVGGSGALTYYYVISFYNNNAEYMLGPEQSADLMQTLKLEVKYMNNYRVLV ESSKLNQSYMVDVSDRKEFYEGMVYNSEGKLISPLEGFVIYQPHLYPIKTDGIAPYKL QAQNDIAGTSHADRLGYTVTYWKYDRQSKSWGLDPEYFWVMLG" gene complement(16823..17296) /locus_tag="ClothDRAFT_2612" CDS complement(16823..17296) /locus_tag="ClothDRAFT_2612" /inference="protein motif:PFAM:PF00583" /note="PFAM: GCN5-related N-acetyltransferase; KEGG: ppr:PBPRA1972 acetyltransferase" /codon_start=1 /transl_table=11 /product="GCN5-related N-acetyltransferase" /protein_id="ZP_05430751.1" /db_xref="GI:256005799" /db_xref="InterPro:IPR000182" /translation="MIVLRDVAGSDESMYQALQSDLDLTDYLSRFCPYNSSVANYDTS KVCWFIIEEDTNCIGSIWLERDAQEPDTVILGIFISGKNSRGRGIGSYAIKEAIRISK QRMPFRYVRLNVRRNNLRAIRCYEKCGFKVCGEGQKNAKNGAIIDYYRMIMKVED" misc_feature complement(16826..17293) /locus_tag="ClothDRAFT_2612" /note="Acetyltransferases, including N-acetylases of ribosomal proteins [Translation, ribosomal structure and biogenesis]; Region: RimL; COG1670" /db_xref="CDD:31856" misc_feature complement(16904..17146) /locus_tag="ClothDRAFT_2612" /note="N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of an acyl group to a substrate; Region: NAT_SF; cl00443" /db_xref="CDD:185998" gene 17781..19436 /locus_tag="ClothDRAFT_2613" CDS 17781..19436 /locus_tag="ClothDRAFT_2613" /inference="protein motif:PFAM:PF07656" /note="PFAM: KWG Leptospira repeat protein; KEGG: geo:Geob_0055 KWG repeat protein" /codon_start=1 /transl_table=11 /product="KWG Leptospira repeat protein" /protein_id="ZP_05430752.1" /db_xref="GI:256005800" /db_xref="InterPro:IPR011647" /translation="MKKHLLIIVSALVSVLSLNLIAVTAAEPTVKNIYYDDVYSFSEG MSRVVKNGKYGFMDQTGNVAINLEYDYVRDFSDGFAAVSKGGTWYDEEGYVDGKWGFI DSTGKVVVPIIYDKVCDFSEGLAAVVKDGKLGFVDTTGKVVIPLTYDCSMYEEFYFNH GLAVVAAGDFEDPDIFVIDENGNTAFDFKYDYARLSGYSEGMLAVAVGGDWGIGGPYY WARSYNFGKYGFIDTNGNEIVAPVYDYASDFREGIAVVCKDGKWGAIDENGDVVVPII YTDISFPSNGLLCVCNDEGKWGYVDYTGKVVADFKFDLCNTFREGYVTNSIDGKWGAL DTTGKTVIPFKYYNLWNFSEGLVRVRMVEDGKWGYMDAGGNIAIDPVYQAATDFSDGV AIVVKDGKFGIISKTSIPYTATPTTSTVLVNGSPVAFDAYLINGNNYFKLRDLAYILS GTDKQFEVTWDDTLKAINLISGKPYTVSGGEMATGSKNTATAYPSAATVFINGVRVEL TAYTINGFNYFKLRDLGKEFDFGVIWDGTAKTIRIDTSSSYSE" sig_peptide 17781..17849 /locus_tag="ClothDRAFT_2613" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.539 at residue 23" misc_feature 19080..19409 /locus_tag="ClothDRAFT_2613" /note="Copper amine oxidase N-terminal domain; Region: Cu_amine_oxidN1; pfam07833" /db_xref="CDD:149091" gene complement(19693..19878) /locus_tag="ClothDRAFT_2614" CDS complement(19693..19878) /locus_tag="ClothDRAFT_2614" /inference="similar to AA sequence:KEGG:Gbem_0692" /note="KEGG: gbm:Gbem_0692 metal dependent phophohydrolase" /codon_start=1 /transl_table=11 /product="metal dependent phosphohydrolase" /protein_id="ZP_05430753.1" /db_xref="GI:256005801" /translation="MEKVLFAVDELTGLIWAAVLMRPSKSTKDMELKSLKKKYKSKGF AAGCSREVIERGAEQLG" misc_feature complement(19696..>19878) /locus_tag="ClothDRAFT_2614" /note="Metal dependent phosphohydrolases with conserved 'HD' motif; Region: HDc; cl00076" /db_xref="CDD:185790" gene complement(19924..20313) /locus_tag="ClothDRAFT_2615" CDS complement(19924..20313) /locus_tag="ClothDRAFT_2615" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430754.1" /db_xref="GI:256005802" /translation="MGRVIYMFFFAIFGIQDKNKHIGNCNNIVCPSCGRLSMYEVYKY YKYFHIFFLPIFKWDVRYIVKTSCCGCLYELDPLVGKEFEKNPSTEIREENLQRIDRY SPFKYCSNCRTEVPVEYSYCPYCGERL" gene complement(20479..21153) /locus_tag="ClothDRAFT_2616" CDS complement(20479..21153) /locus_tag="ClothDRAFT_2616" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: ccv:CCV52592_1508 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430755.1" /db_xref="GI:256005803" /translation="MTKVQKRLFEVADKQYALFQAKLIPNIPQERVIGVRVPVLRKIA REFEKEPECRDFLNTLPHAYYDENMLHSILLSNVKNYEECIAAVEAFLPYVDNWAVCD TLRPKVFGKHKDKLLPRIKDWIASDKTYTCRFGIGMLMMHYLDKDFKPEYLELPQKVD SEEYYVKMMIAWFYATALANQWDATIPYIEEHRLSDWVHKKTIQKACESYRITDEQKA YLKTLR" misc_feature complement(20542..21075) /locus_tag="ClothDRAFT_2616" /note="A new structural DNA glycosylase; Region: AlkD_like; cd06561" /db_xref="CDD:132880" misc_feature complement(order(20638..20640,20659..20664,20755..20757, 20848..20850,20860..20862)) /locus_tag="ClothDRAFT_2616" /note="active site" /db_xref="CDD:132880" gene complement(21332..22117) /locus_tag="ClothDRAFT_2617" CDS complement(21332..22117) /locus_tag="ClothDRAFT_2617" /inference="protein motif:PFAM:PF00005" /note="PFAM: ABC transporter related; SMC domain protein; SMART: AAA ATPase; KEGG: pfo:Pfl01_0242 putative amino-acid ABC transporter ATP-binding protein YecC" /codon_start=1 /transl_table=11 /product="ABC transporter related protein" /protein_id="ZP_05430756.1" /db_xref="GI:256005804" /db_xref="InterPro:IPR003395" /db_xref="InterPro:IPR003439" /db_xref="InterPro:IPR003593" /db_xref="InterPro:IPR017871" /translation="MAFLEVKGIEKSFGTVKVLKKIDFSLEKGEVIAIIGPSGSGKTT LLRCLNFLERPDKGSIIVNNEVIFDAMDPSTQRESEVRKKRLHFGLVFQNFNLFPQYT VLKNVTLAAELLAKERPDYKQNKKAILKEIQDRGKMLLSQVGLSDKLDCYPHMISGGQ QQRVAIARALALDPDILCFDEPTSALDPELTGEVLKVIKGLAERNTTMIIVTHEMDFA RDVANRVIFMDGGVIVEQGSPQEVFGNPKEERTRQFLARYKKH" misc_feature complement(21344..22108) /locus_tag="ClothDRAFT_2617" /note="ABC-type polar amino acid transport system, ATPase component [Amino acid transport and metabolism]; Region: GlnQ; COG1126" /db_xref="CDD:31323" misc_feature complement(21419..22108) /locus_tag="ClothDRAFT_2617" /note="HisP and GlnQ are the ATP-binding components of the bacterial periplasmic histidine and glutamine permeases, repectively. Histidine permease is a multisubunit complex containing the HisQ and HisM integral membrane subunits and two copies of HisP; Region: ABC_HisP_GlnQ_permeases; cd03262" /db_xref="CDD:73021" misc_feature complement(21989..22012) /locus_tag="ClothDRAFT_2617" /note="Walker A/P-loop; other site" /db_xref="CDD:73021" misc_feature complement(order(21479..21481,21575..21580,21839..21841, 21986..21994,21998..22003)) /locus_tag="ClothDRAFT_2617" /note="ATP binding site; other site" /db_xref="CDD:73021" misc_feature complement(21839..21850) /locus_tag="ClothDRAFT_2617" /note="Q-loop/lid; other site" /db_xref="CDD:73021" misc_feature complement(21623..21652) /locus_tag="ClothDRAFT_2617" /note="ABC transporter signature motif; other site" /db_xref="CDD:73021" misc_feature complement(21575..21592) /locus_tag="ClothDRAFT_2617" /note="Walker B; other site" /db_xref="CDD:73021" misc_feature complement(21557..21568) /locus_tag="ClothDRAFT_2617" /note="D-loop; other site" /db_xref="CDD:73021" misc_feature complement(21473..21493) /locus_tag="ClothDRAFT_2617" /note="H-loop/switch region; other site" /db_xref="CDD:73021" gene complement(22132..22794) /locus_tag="ClothDRAFT_2618" CDS complement(22132..22794) /locus_tag="ClothDRAFT_2618" /inference="protein motif:TFAM:TIGR01726" /note="TIGRFAM: polar amino acid ABC transporter, inner membrane subunit; PFAM: binding-protein-dependent transport systems inner membrane component; KEGG: dia:Dtpsy_0712 polar amino acid ABC transporter, inner membrane subunit" /codon_start=1 /transl_table=11 /product="polar amino acid ABC transporter, inner membrane subunit" /protein_id="ZP_05430757.1" /db_xref="GI:256005805" /db_xref="InterPro:IPR000515" /db_xref="InterPro:IPR010065" /translation="MNEVLVIVGRLSESFILNCKLFILTLLFALPLGMIIAFGSMSRF YPLRWLVRTFVWVIRGTPLMLQLIIIFFGPGLLNMPFSWPSGESGRFVAATVAFVINY ACYFSEIYRGGIESIPVGQYEAGQVLGMTKTQIFFHVTLLQVIKRIVPPIGNEVITLV KDTSLARIIANKEIIMMAGEYSNKGLIWPLFATGIYFLVFVGGLTILLNWVEKKLGYF RV" misc_feature complement(22189..22698) /locus_tag="ClothDRAFT_2618" /note="Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits...; Region: TM_PBP2; cd06261" /db_xref="CDD:119394" misc_feature complement(order(22189..22191,22198..22203,22210..22215, 22219..22224,22231..22236,22264..22269,22291..22296, 22303..22314,22333..22335,22342..22347,22387..22389, 22438..22440,22447..22452,22462..22464,22468..22473, 22480..22482,22486..22488,22492..22497,22582..22584, 22588..22593,22600..22629,22633..22644,22672..22674, 22687..22692)) /locus_tag="ClothDRAFT_2618" /note="dimer interface; other site" /db_xref="CDD:119394" misc_feature complement(order(22297..22314,22582..22626)) /locus_tag="ClothDRAFT_2618" /note="conserved gate region; other site" /db_xref="CDD:119394" misc_feature complement(order(22234..22236,22264..22266,22273..22275, 22294..22296,22510..22512,22582..22584)) /locus_tag="ClothDRAFT_2618" /note="putative PBP binding loops; other site" /db_xref="CDD:119394" misc_feature complement(order(22366..22368,22378..22383,22399..22437)) /locus_tag="ClothDRAFT_2618" /note="ABC-ATPase subunit interface; other site" /db_xref="CDD:119394" gene complement(22878..23687) /locus_tag="ClothDRAFT_2619" CDS complement(22878..23687) /locus_tag="ClothDRAFT_2619" /inference="protein motif:PFAM:PF00497" /note="PFAM: extracellular solute-binding protein family 3; SMART: extracellular solute-binding protein family 3; KEGG: rfr:Rfer_2969 extracellular solute-binding protein" /codon_start=1 /transl_table=11 /product="extracellular solute-binding protein family 3" /protein_id="ZP_05430758.1" /db_xref="GI:256005806" /db_xref="InterPro:IPR001638" /translation="MKKLKMKKLISLLLTLTMILALTACGAKETGKSQDESDLNYVKS KGKLIIGYTNYAPMNYTDENGVFTGFDTELAILTCEKLGVEPEFVEINWDTKEVELNA KSIDCIWNGLTIDDDRKAKMEITQPYVKNAQVVLVKEGTEYNGTESLIGKTVVAEQSS AGEKTIMADENLKKANYVPKTLQTDCLMELKSGTADAAVLDLTLAKTMTGEGTSYEDI VIVDYLAEENYGVAFRKGSDICAEVNKIFDDFLADGTMAALAEKYGLELAE" sig_peptide complement(23607..23687) /locus_tag="ClothDRAFT_2619" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.555 at residue 27" misc_feature complement(22890..23546) /locus_tag="ClothDRAFT_2619" /note="Bacterial extracellular solute-binding proteins, family 3; Region: SBP_bac_3; pfam00497" /db_xref="CDD:144186" misc_feature complement(22896..23546) /locus_tag="ClothDRAFT_2619" /note="Bacterial periplasmic transport systems use membrane-bound complexes and substrate-bound, membrane-associated, periplasmic binding proteins (PBPs) to transport a wide variety of substrates, such as, amino acids, peptides, sugars, vitamins and...; Region: PBPb; cd00134" /db_xref="CDD:29040" misc_feature complement(order(23085..23087,23205..23207,23334..23336, 23409..23411,23523..23525)) /locus_tag="ClothDRAFT_2619" /note="substrate binding pocket; other site" /db_xref="CDD:29040" misc_feature complement(order(23106..23108,23124..23126,23136..23138)) /locus_tag="ClothDRAFT_2619" /note="membrane-bound complex binding site; other site" /db_xref="CDD:29040" misc_feature complement(22992..23009) /locus_tag="ClothDRAFT_2619" /note="hinge residues; other site" /db_xref="CDD:29040" gene complement(23887..25410) /locus_tag="ClothDRAFT_2620" CDS complement(23887..25410) /locus_tag="ClothDRAFT_2620" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: ypb:YPTS_1104 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430759.1" /db_xref="GI:256005807" /translation="MSAQSIYSVIYEGINSEGKLPKDFTLPFKKTFPNELKFMPGAKD GIGIFHFGAKSSEKVSKKIVKLLKSDCKKGSISSQNEIAELLNECGTLAVIDPILDSI RKEQKKFDIENLFDYACQFAFQSTDEELVKLGIGLLGLFDLSNDDKIIDKLLVLALYE EFTLYVIVAVLKYPNGNDIVFRIAQKVDGWGKIHAVERLEPTSDEIREWILRKGCANE IMDAYLGLECGNKGNLIGALRHGSIDDELFEGISVIIDALLDEGPVEGISVYEYAEEA LRLYLQIASEHAVTITQFWRILNLQDWLINAQIAGRDELLKMCGNIINKESWREMILK ILNSSDDERFFYAYDAAKRLNMDISELIFKAVKRNPVKRCGYLSIVYKNPEYANELTK IYEEILPLDEMATGMGDFIFAESLTEEHLCMVFVLEELKNYPKMGEKLVRTALKSPVI RERNGACIVMKEWCRILNQDLQTISPDLFSTLKKIVDIEVNADTKDNMRELLNITPE" gene 25825..>27383 /locus_tag="ClothDRAFT_2621" CDS 25825..>27383 /locus_tag="ClothDRAFT_2621" /inference="protein motif:PFAM:PF01266" /note="PFAM: FAD dependent oxidoreductase; Rieske [2Fe-2S] domain protein; KEGG: mxa:MXAN_1554 Rieske family iron-sulfur cluster-binding protein" /codon_start=1 /transl_table=11 /product="FAD dependent oxidoreductase" /protein_id="ZP_05430760.1" /db_xref="GI:256005808" /db_xref="InterPro:IPR000447" /db_xref="InterPro:IPR003042" /db_xref="InterPro:IPR005805" /db_xref="InterPro:IPR005806" /db_xref="InterPro:IPR006076" /translation="MSIEQQKIFAKPPQSYWMASTPKANYPTLEEDIKVDVAIIGGGI TGIATSYMLGKAGVKVAVIEADRILQGTTGHTTAKITSQHDLIYSKIYSQMGRELAQQ YADANESAIRMIEKIATENGIECDFVPQSAYVYTMQDKYIDKIKDEAVIAESLGIKAT YLEEIPLPFPIKAAVRFDNQAQFHPRKFLLRLAEEIVKSGNQIFEQSRIVDIEDDNNY VLITNQGKKVTAEKLIIASHYPCYNKAGLYFTRLYPERSYVVAIKAKESYPGGMYINM EEPKRSLRSQRSDDGELILVGGESHKTGQGEDTIKHYEALIDYATKTFTVEDIPYRWS TQDCMTLDGLPYVGHFTSNTPNMYIATGYGKWGMTNSIASAMILRDLIIEGKSPWQDV YNPSRKTVLASAKNFIVENLNVAEKLIEGKILPMADNTDIKAGEGKIINVNGQRLGAY RDQQGTLHVVDTTCTHMGCELYWNSAEKSWDCPCHGSRFTYEGDIIEGPAVTPLNVHR DVNTIEKLFKD" misc_feature 25918..26991 /locus_tag="ClothDRAFT_2621" /note="Glycine/D-amino acid oxidases (deaminating) [Amino acid transport and metabolism]; Region: DadA; COG0665" /db_xref="CDD:31009" misc_feature <26335..26982 /locus_tag="ClothDRAFT_2621" /note="Pyridine nucleotide-disulphide oxidoreductase; Region: Pyr_redox; cl14644" /db_xref="CDD:187400" misc_feature 27097..27369 /locus_tag="ClothDRAFT_2621" /note="YhfW family, C-terminal Rieske domain; YhfW is a protein of unknown function with an N-terminal DadA-like (glycine/D-amino acid dehydrogenase) domain and a C-terminal Rieske domain. The Rieske domain is a [2Fe-2S] cluster binding domain involved in...; Region: Rieske_YhfW_C; cd03477" /db_xref="CDD:58547" misc_feature order(27214..27216,27220..27225,27268..27270,27277..27279, 27283..27285) /locus_tag="ClothDRAFT_2621" /note="[2Fe-2S] cluster binding site; other site" /db_xref="CDD:58547" ORIGIN 1 ctccttcccg gtaagaatca taaacaataa aaaccgggaa ggagtttatc cagtcttata 61 cgcatgtata cccaccgtct accggaataa cagcgcccgt aacataggaa gatgcatcag 121 atgcaagata gacagcggtt gaatccaact ctccttcttt tccgtagcgt cccataggaa 181 cagtggattt catgtaattg gtgaaatatt cagtgttaag aacgtcctcg gtaagctccg 241 taacaaaata acccgggcat attgcattta ccgtaatgcc gtattttgcc cactctgccg 301 ccaaagctct tgtaagattt atcacaccgc ctttggccgc atgataagca gcgcttggat 361 gtgccatgtt accaaccatg ccgtatatgg aagaaatatt aataattctt ccatacttgt 421 tttttatcat aattttgcca aattcccggg caaccataaa aactccgctg agatcgacat 481 taattgtatt gctccacatt tcatcgcttg tttcctcagc cgaggctata ccaccgctgc 541 ctgcattgtt tattaaaatg tcaacttttc cgaattcttt ttcggcaagc tcggcagctt 601 ttctgaccat ttcgattttc gttacatcac actctatggg aaggcatctt actccaaatt 661 gccggatctc ttccgcgacc ttctcaagtt tttccttgcg tctcgccaaa ataacaatat 721 cagctccctg ggctgcaaga gctttggcca tttgaacacc aagtcccgat gatgctcccg 781 tgactactgc aactctgcct tttaaggaaa acaaatctct tttttgcata ttaaacacct 841 ctttattttt tgattcattg aaactgtgac caatatatat aataccacca ttgaaaaagt 901 ttaaataatt tttttgttaa aaatataaca aaataaaata atccatattc gtatggatat 961 tgtaaaatac ggatatatat tatttataaa gcaacggata gtttctgatt gacatgaatt 1021 ttatgacttc tcaagcattt ctttaacaat cttgtattct tgagatcgcc tttaaatatt 1081 attcgtccgt ttaattttaa aagactttga ggtcttacaa gatttcttga tattttaaag 1141 tctgcaatac cgctctcatt caagatataa tagacaaatt cagaacagac gaatcttgaa 1201 tcgtgacatg cttccctgtt tagcaagccg ttgacaagtc ccatataatt gtatttgtat 1261 cgcccggcat tggaaataaa acattgcagc agggctttag ccctttgata ctgctgtttt 1321 gtcacttcga gctcaattac cgctccgggc aacactttac agtatttata cgttcctttg 1381 tttaaatttt catgcttgaa tacaccgata aacggattga aagtatattt tcttccgaaa 1441 ctatacatgt ttgcaaggtc tctgtccaaa gaaatggcgg cgtgggtgaa ttcatctttc 1501 ttgaaaagtt gtataagtct ggatattacc gtatttgtcc ttgtaaggac tacgtaaaca 1561 taaaactttt ccattatact cccccataaa ccatcgaata tacacaaaca caattttatt 1621 cgacatgaga tactaaaaaa ataataaaat aataaacgtt ttttgagatt tttcagttat 1681 aaattaatta tattcgttta aggaggcaaa atcaacatat cccggattat caaacaactt 1741 gccttataaa gagcgccctc cgtttaaaat gtctattttt gtaatattta tgcatctatc 1801 ttttttaacc tgcattttta caatccaggg cattatcagc ttcattcgtt caaaatcatc 1861 atacccgtaa gtactgtcat agtaatttat tatggtgctc aatgcagtcc catgtgtccc 1921 aattactatg ttttttcctt catacatgcg taaaacctcg tttagtgctt ttatattccg 1981 attttgaact tctctcaaac tctctccgtc cgacagcttg taatcaaaat cttcccattg 2041 cttttttgca aaagaattaa agtcctctat ccaaacgttg cccacttttc gttctctaaa 2101 atcgtcaaca atttttatat caagtccaac ttcaccggca aaatccttca ccgtatcaat 2161 tgcccgcaaa tacgggcttg atattaccac atgaatatct ttgtcgctta aatacgacga 2221 aaccaatttg gcatcttctt tacccttttt agtcaacggt ctggcaaaat catcccggac 2281 attgtaatcc ggttccgcat gcctcacaaa gtatacattt gtcataatgc tctctccaga 2341 aaatatttat ttacattata tcatataaaa ttattcagct cgtatcacct acaccctttt 2401 atccggttgt atatgtatct acacgccact ttccgtcatc atctttaaca accacaacaa 2461 aaaaaaattc atcccccaca cgtcgccgtc gtgaacaaaa ttattattat ggttttctgt 2521 acttttccca gatacttgag cacattggct gaaaaacacg aacttaaggg agtatttcct 2581 gaagcttttg ctaaaatatt aaaatcataa tctactaaaa gcaaaagtga aaatttttcg 2641 ccttctccgg aatatttttc agttaacacc cagtaaccgc tgccaaaatc ttttatgtta 2701 tatattacaa gagaaccctc attagtcgga tccgatttca ggtagtattc gcttataact 2761 ttggtgagtt tttttctttc ctttgctgaa attgtccctt cactggattc atttgtggca 2821 aaaacaatcg aagctgtgag aacagctgca accgcaacta ttacaaccca aaactaagga 2881 aacggagaag aataataccg atggctacat aggatgcaat tattgtaata gacttaattt 2941 aagtctatcg tgttagacct catatatcaa tagcaaaaat ataaatttaa aaacttttca 3001 aagggtttta ttgtactttc ttattaacaa aatatataaa aatgagttgc cgaattagcg 3061 gcaacccatt tcttaattat attgcttttt atttcattca ttttatttac ctgatatact 3121 cattgctaac caaaatatcg gctattactt cggttggaat ctcaaactcc tgaattccca 3181 tgtatcccgg tccgacttca tatttgtcaa atgaaattac caatttccct tcttccgtaa 3241 tatagaaatt ctgatttgga gatattttat taaacggctc gataaattct tcattctcat 3301 ccacatccac ccagtaaata aaaccgtcat cggctttcat tctctctctc atttgatctt 3361 ttatattttc acttatgatt tctatataac tgttatcttt aaacaaactt ggcagggtta 3421 tcaaaattcc ttccttcttg tcaacagtat catatttgaa taccgttgaa gaagaacctg 3481 cgacggttaa aacatagcgg cctatggaca atattctatc cgtatcagtt ttcacaacgt 3541 atccgctgtc tactccaaga taaccgcctt cttctttctt catattttcc atctcggcca 3601 taaattcttc atacaatttt ttgttttcag cgagatattt ttcgttcaag ctattttgca 3661 attccttgtt ttccaaacct tctatagcag gcactttaat atctgcttta aatctgtcct 3721 catttacagt atactcttta aaggtaagga cttttactat acctcccaca atcggaactt 3781 tctccaaagc agatgcaaaa gccgggctgc tgttaattcc aaccgtaagt actcctgcga 3841 aaacaacaat caaaaccgct gccacgcttg ttctttttat caacttattt cttttcattc 3901 tatgctcact cccgtctttt aatgcttttt tcactacaga atccaactct tccggtatgg 3961 gtgtgtgtaa atattgcttt ttaagaaaat ccaaattttt atctttcata ctaaacctcc 4021 tcacattcgc ttaactttac acgcagtatt tcaagggact tgtataaccg ggtcttcact 4081 gtattaacat tttcattgag gatttctgca atttcatcta ttttcaagtc ttcaaagtat 4141 ctcagtacaa ctatggtacg atatttttcc ggcaaattgt ccaatgctct ttgcaagtca 4201 aaatccccat aactgtcaac tgctccaaaa tcaaaacttc ccaaaatctc ttcaccgaca 4261 gcaacgtttc ttttttgcct gcgaagaaag tccagtgagg tattaaccac tattctgtaa 4321 aaccatgttt ttaggtaatc gggatttttc aatgaatcca tagatgaaat tgccttgtat 4381 acagattcct gaacaatatc cagagcatca tctgcatttt ttacatagct gtatgccagc 4441 ctgtaaaaat tctccttgtg tttaacagca tattctgcta tttgctcctc caggtattta 4501 ttctttaata acacttaagt tgtcatccct tcttttattt tggatacgta tacgtatctt 4561 aactgctgta cactttatag acgtatggat acatataaaa agttttagat ttttagcttt 4621 tattaaaaat taaaatatat caaaatatta aaaaaatttt taaaaaaata aatccccctt 4681 cggattgcat aggaaaaatg ctgcatatta ccatgttcca ttataaagtt ttattctttt 4741 tcttttttcc ttccttttcg gcatcaacac gtactacatc aacacgatga ctgtgacagc 4801 tcaagccggt gcagcggtgc aaaagctcca tgatttttgt ccgttcaccg gattcccgat 4861 agctttccaa aagagtttcc gctgcctgaa gaaagatatc ctcgcagctt aaccttgtac 4921 tttccgccca caaagcttcc atgtcgtcaa gataacgtcc ggtgtaacgg tcaaccactt 4981 tttgagcatt ttcaggagtg ggatttctcc gaaattcgaa ggccgcctgc cggaaggcgt 5041 catagtcgca aacaatttcc tccatgtcca gaaagtatat ccctttttta tgcttaatgg 5101 ggtttttggt tccaaggctc tcaaaggcac gtcagatttc accccgtcgg gtgtggaaca 5161 tgccggtgac atctccctca ctgtcctgct acagatctgc aaaaatcctc tctcggctta 5221 cgtcttccct gtgttccagg agataggcca gtagttccct ggatttctgc gtgcggattt 5281 tgacccggga gcatcggtca tgagccggag caatataaaa agcacccaat gtatttatgt 5341 aaactttttt gggctgtgta accgccgtat gcaagcattt gttttcggcc atgtcaaagg 5401 atatctttaa catcgtgcag agccttattg ggccggatta aaacctcgga agtcaagcgc 5461 agtatttttg caaagagtaa ataatcttcg gtactgcggt ttaaggcagc atgttcctcc 5521 gccatctcca gataccttcg aaaagctaaa aaatcggcaa aactcccctg gctgagtttg 5581 cgtatttcct cggtcaggag aatttcagca tatatcaggt aattgaggct cagctcatca 5641 taaagcccta agcgtcgaag acgggaaatc tccgattcca tcaatggaat ggacctttcc 5701 tcctgtcctg ttagctgata ggtcttggct gcataggccc ccatacagtg tcgtgaaagc 5761 cagtcctgtt cttcctccgg gagggacagg gatttttcgt aatattgaag gcactttatg 5821 taatcaccct gataaaaata aataacagtc agatagcact cgagccaagc tctgacccgt 5881 gagctgccct gtaccgcaca tttctctgcc atggccttgg tcagtaccaa agcctccgac 5941 aggcgagtag ctaaagtcag gttatagatt ttctctatca tcaccatgta ccaatcttcc 6001 atagaagcat cctccggcaa aggcagaagg ctgtcgagca aacggctgct ctcctccagg 6061 gaaactccat aagataactg tataatttcc gatcagcctg gggcagacaa agcttcccgt 6121 tctctgctgc cacccggtag gactgcagga ctagtgccca tccctctgtg gcagtatagg 6181 cagcctcgtc aaaaaaaccc cagagctttt gtgcttcctc tcggctaaag cacagatcag 6241 ccttggtgag ttcgacaatc cgtccttcca tcttgagccg gaaaagtccg ctccacagtt 6301 catagcggcc ggccatgaca agggtcagac ttgaaggaca gctcttgacc aatctgttta 6361 aaaagtccat cgtcgcaccg tttgtaataa catggacatc atccagaatc agggtaagct 6421 ttcggcgtcc tatggcgcaa agcaatgccg acagtgctct ggagacaaaa gtatccctgg 6481 ctgcgaaagg aagcagatcg gtggcgtaaa aatcgaagcg gggaaacttt ttgcgaaagg 6541 cgtcttccaa atggcgcaaa aagtatgtca tattgctgtc cctctcgtca agggaaagcc 6601 agaccacatc gttccgttca ccggcatact gcatcatgag ggtggttttg ccatagcccg 6661 catcggcatg aatatatgta agactgctgc cgggaggagc aattttttcc gtcagacgtg 6721 gcctgggaac tatcgcgcaa ggtactttgg gcaaggaaaa agcatcctct ggcttttccg 6781 ccgtttcggg gattaaccat attctgccgt gacgttccgc accttttatc ttgccctgtt 6841 tacacagaat ctgcacgcga cgcaccggaa ttccccattt ttccgccact tccttcggta 6901 tcaaatatct cataaatcct cccacgcatt aacatgaaac tttaatttaa tcatatacgc 6961 ttaagcgaac aatatcaaaa aaaataaaac agacccgcgg aacaatccca caggccggtt 7021 attgtattac tatcttcctt caaacatttt aagcaatcgc aaataatgat tcgcttctct 7081 taaaacatga tcacccagaa gcggtatgat tatcgatttt actttacatt caagaatcgc 7141 ctgagtgcct tgggctttga agttccgaaa atcctcggtt gcttttaagc tttcgtcagt 7201 aactttagcc attggagagg ttgcatccat tgctgccttt gcttctgcag tcaattgatc 7261 aaactcattt ccaaaatcat tggcctggtt aatcagatcg ttttcagtag ggtcaagcaa 7321 ccctcggata aacttggcat gttccgccat ttgcctgttc cagaaaaatt gtaattcgta 7381 agcttctttt gccaaatcta tctcttctct tctttgaagt ctcctaagct gcagaagata 7441 caattctgcc tcacgagtta tgtgcaggat caataaggga tagttaaatg taaacatttt 7501 gcagtataaa acattagata agatattctt tttaaactgt attaatgcag cagttgcatt 7561 tattgccctt tggttaagca cggatacatg tcgttcaagc actggattgg ctgttattat 7621 atcgctgcct gccagtcttg cttctgcctg cgtcagttga gttggtatcc gtacccctgt 7681 aaaatatatg gttgccattt ctgcattcaa agtaaaaggt gttattacct ctccagattg 7741 aagtatctcg ggattaacga cactgtttga aagagaaata gtttctccta aaattccgtc 7801 aaattccctg cgaaaagcat ctgcctgctg gataaagcct gaatctttcg acgtaaaccc 7861 cacttcaagg aaaaatgagt gctctttcat aatccttgca aaaaacagat gtaaacccag 7921 agactgtttt ataaattcaa tacttgaaag catggcttgt tcctctgcat aaacaggtca 7981 actcctaata tcagtttatg ttaacatcct atgaattgtt acggaatatt gcgttcaata 8041 acaatagtta ctataggcga aaaccttaaa attgaaattc tttactttct tttttccgga 8101 tttctcaagg cccacaatat gtagcacttc ttcagaccct accttggatt taattaaagc 8161 agaataggca attataagga atttataagg attcgctatt ctcgtaggag gttactttta 8221 ttaattggaa tttgagtttt ggatagaaat aaaacaccgg aataaaacaa aagccggaat 8281 aacccttgat tttcggttta ttccggttaa tttccaaaga tatatggaat ttgccttact 8341 cgtcaattac aacacaggaa tatattaact cacctgccac tcctcgttca tcaggatact 8401 gtctctcgct tttactttca aacgaaaggc ctattttctc catgagacgt gcagaattaa 8461 gatttcggca atcacaacgg gcaagcagtt ttttcacttt taactgattg attgcaaatg 8521 acttaattgc aagcgcagct tcagtagcaa atccccttcc ccaatatttc ttattgagaa 8581 tccagcccaa ctcgccttcg gttctatcct catttagata tacgcaaact gctccaatat 8641 gtacagagtc atatacaata gcaaattcat aataagatgg ttctttcttc tgccattccg 8701 cactaacgac ctctaaaaag ctatgtattt cttctatact tttatgagga aaatgaatca 8761 tatatatttc gtattttctg catatgacgc atattcgtat actgtatgta aatctaaaac 8821 actgaacgga cgtagcaaca atctttcatt tcttaattca atatacatta gttactttcc 8881 tttcttataa atgctgttgt cttaacgtga ctgaagttga tgaatgtcaa cgtattgact 8941 gtttaaggta aagcattaat aacgcattta ctgacaacat gaagacaaaa taatgtcaag 9001 ttgataatgc acacccttct tttaattaaa atattccttc catgccggta acttgtgcat 9061 catattttct gcaattttcc aggcaacgtt ttcatctgct ccctgcgttt ttactataaa 9121 tttggtaagg ctctctttct tctcttcaaa agactgcatt tctccgtcgg gacgtgaaca 9181 gtaaacacaa taatctttgt tgggatcgtt tgccgcaaag tctgaaatat ccttcatagg 9241 cattccacaa gcaatacacg ttttcattca tattcctcct tttcattttt aaatatatac 9301 tttaggtttt cataaatata attttatgtc ttgcccaaaa tatcaggaca tttacataga 9361 aagttctgta ttgtgagtag ataaaaactt gaagttatta gccttttgaa agcctaagct 9421 atatagttct agatacgtag acagtaattc ctgtccgtat ctttttataa ctgactcatt 9481 agaatccgag aaaaaacctt attcatcaga taataatgaa ccagacaagc catgtattat 9541 acagcatatg agtgggtagt tttttcactg ttcctttctc tatttcccgt tctatgacgc 9601 tgctgaaatg gaaatataca aatcaattac gtgcaatcat atgaacaata taaccgttgt 9661 tttgcacgat ataactattt taccacaaca tgccttacat ttaaattaat ttatcattta 9721 agcaattcat ttaatgtgtg tgtttcccca attttaaaaa attcttttcc aatcaataga 9781 aactcttgta tttttaataa tcatacaata taattacatt aatattgtaa caaacttgta 9841 acatttaaat tttctcaaaa tattagtaat actataacac ataaagtaag ggtgattaaa 9901 ttgatttatt tttgtcacgc tccaaacgac agagctattg cggaaaaaat atatttttcg 9961 cttaagcaaa cgggtcttac atgttggatt ccgtcacagg atattatggc cggtcaatac 10021 tatgtggaag ctatagccaa cgcaattgaa aaatccgaca tcgtagtttt tattttttct 10081 tcacactcaa atacatccat acaagtaatt gatgaattgc agaaagcatc atcactgaat 10141 aaaactatta ttcctttctg cgttgacagg gcaatgccct cagagccaat tgaacactat 10201 ttgagcagcc cgtacaaagt cgatgcaaca atcggtcttc cagatgataa tattgcaaag 10261 cttcagaaca ttattaagca aatatataac caatcttccg tacataatgc taatgacatt 10321 aatactgtta aagatacatt tgcgccatcc aatccattag aaaataatac aaacatcata 10381 aaagatgctt cggataacaa cagccaagag tcaatccaat ttaacggggt aattaattca 10441 gctaattctc aacctccaca aaacccaaat ttgcctccac aacaccaaat gaatcctaac 10501 tttaacaaca cagtcaataa tatggacaga aatatgaact acccaataaa gcaaaatgtc 10561 cgtcccaatc ctcaagttaa atcctccaat aaaatcaaaa ttccccttat tattggcgga 10621 agtattgcag gaggaatcat attattgtca attttattca tgcttgggaa aaacctatta 10681 ttttcgacat taataaactc gcacagaccg tctgacaatc cagtaatagg ttcaaacatt 10741 cgtattgacg gcagtaaata taccgaagaa gaaattattg aatttacaaa aaatgctatt 10801 ttagagctgg atagattgca agcctctcgt gaattttatg atacctatgc atataacgca 10861 ccggaatatg atctaatgcg tattcaggaa aacgtattct tttcacttat ggatttggat 10921 gtggtgagat ttgaggaagt agctatttta ggtaaaaacg aaaaagaaca cggcatagaa 10981 tatctcctca aagtagattt tgtctgctat gcggattatg aatatacgga gaataatgaa 11041 actgttcacc gcaaaggaga agcattattg tacaataatg tggtaatact tgaaacacca 11101 aatgacggat taaaatactt atacatggaa ggaatatttg aagatgagtt aaatgcaaga 11161 aatgagaact tggagtcttc atatcgtgaa atcgccgaat ccggtgatgt aaacactgaa 11221 gacaccatcc aaacctactt gcctgattca accgacgaag acaccattat gtccgtaaaa 11281 gatatcgtta agaaaaatga ccacaaagtt gttgccgtgt acgttgatgt accgggagga 11341 caatctcaag gaagcggatt ttttataaaa gacggtgtaa tagttaccaa ttatcatgta 11401 atcgagggcg gaaaaagtgc aaaaattctc ctttccaacg gaaattacgt ggatgtggaa 11461 ggagttttat acacggattc tgatgttgat attgctgtat tgaaattggt aaatgaagtc 11521 ggcatcgagc cggtaaccat aggtcaggcc cgcgattccg ataaaggaag tatcgccgtt 11581 gctattggat cacctttggg actgtttaat acagtatcca caggaattat atctaatttc 11641 tgggaggcca atggagttaa cttaattcaa atttccatcc ctattaccca cggtaactcc 11701 ggaggtgcct tattcaacga gtccggtaaa ttaatcggta tcacatcctc aggaatagga 11761 gaggcaaatt taaacttcgc aatttcttca acccatataa tacctatttg tgaggatata 11821 aaaaatatac cctataatca attaaatgca gttcccctta gcagtgcagg cggcaatatt 11881 aattccatta ccaggcaagc cggatcttcc aatagcaatc aaagcagttc ttccggcaag 11941 agtcttttat cttcaaaata caggtttgtt aattccgata aaccaatatc caacgatgca 12001 acatatacct ctgacttaca gacaatatat gacctggctt taaacaagta ttatgccaaa 12061 gaagatttat acagagatct tgattacgcc tttgactata ttttggatta cggcacaaag 12121 tattatctgg agtatttaga gtatatactt catgaaacat atataaaaga agatgtagaa 12181 aactttaata agataaatga atctgttctg gaagtgctta acaccacagg tgaatatata 12241 gaatttacag cggatgaaat tgaaataata ggtgccggaa taaaaaacga cggcactatt 12301 gacggtatta ttgtgagagc tttattctat gaaaattcgg agccttatgt gagatcaaaa 12361 ttttatttca gagctaacta tgacgcatgg agttatgttg atggcttctt gtatgtagga 12421 gaagttacag aaaagtaatt atttttaagg ggtacagaac atgtgaaatc attggagggc 12481 ttcaggtgga gatttcaact ccacttgagg cttaaatatt aattttttcc tccgctaaat 12541 caaacgggat atgtagatgg attacaatga cttactgagt atataccgtt attttttaaa 12601 accctgtata cctctaaatg aacctcttct tttgttctca ctttacttcc ctcacttttt 12661 tctgttatat cgacccgtta tcaatccaat gctgattgcc gacagaagga gtctcttctt 12721 ttttcaggag tcccttgtaa gtcattttac atatattaaa cagaagtctt attttccttc 12781 aatcaagtct tatcagcaag tttccaaccc aatttatcaa tatacagata tacgcggaca 12841 tatccgtcaa ccggttttgt aaaaataaga gttgaagttc tttgcgtatg tgtttcgctt 12901 actcccaaaa gtgcttttct gtagacggaa tcaaaaatat tgtattgata atcgaaatta 12961 tctttgttta caccaatttt tgaatacatt tgagctctaa aatagtcttc atccatttct 13021 tcaactgtaa tttttttatg cttatcataa taagcaaata tttcatttat cttattttca 13081 ggaaccgcca attcagcctc tatacagtag tgaaactcta tatctttttt tataacaaag 13141 ttcttgatta atgactcatc attaagcttt gtatgaaaat atttatttat cattccttgc 13201 tgagtgccgg ttattctttc aatattaaac ccaattgagg atttgtaata atataaaatg 13261 gggaaaacgg ccaataaaat tataactgtc aagattatta caatcctttt tattttcaaa 13321 gttaacaccc cccataaata ctaccgcatg acacagattt taatatgtat ataaatttat 13381 tcgctcagaa tatatttata ccaatataca aggactgtaa aaacattaaa gcatcattcc 13441 gtctagttca tctcattgtc tgaaataccc attttgcggc attcattaca tacataatct 13501 tttccgcctg atttccgata tgatacttta aaatcttatc cttttgcttt tctcaatttt 13561 actattttct cagacaactt cataattaat cacctccata tcctaataca ataataactg 13621 ataaaatgga ttattaccac caatttcaaa gtttagaatc atatcattta tcagctacct 13681 ctcgcccgga atctaatttt ttaaaattta aggttatcat ggtaagagac agaaggcagg 13741 ccatgaaatc tgctatggga ccagcataaa gaacaccggt cagtcccaga aattgtggca 13801 gaatcaaaag caaaggaatg agaaagaaac cttgccttga taatgaaagt ataattccct 13861 gcctcacatt tcccgtaccg gtaaagtaat tgattgtcac tggctgtata ccaaaaacgc 13921 ataccataaa catataaatt ttcagatatt tctctgcaaa ctcaaagtac agttcatctc 13981 catatagttc atctccgctt ccgaaaatcc cggtaatttg tctcggaaag cattgaaaca 14041 aaataaaagc cagaacacta atacctatag cgatggagac agctttttta taagtttcct 14101 tcactctgga ataatttttc gcccccatat taaaacctaa aattggctga catccttgtg 14161 ccaaaccaat ggaaaaggca gaaagaacac tgtttaattt tgcaataata ccggatactg 14221 ccaatggaat atcgctgccg taaatggaca tggcgccgta gattttcagg ctattattca 14281 aaacaatgtt tacaagcatc ataatggtat gattaatgaa attagaagtt ccaagttttg 14341 caatccgttt tgcataatac cattttatac ccaacattct tcgggttatt tgaaaggttc 14401 tgaacttaaa atagtagatt gcacaaagga gaaaggatac aatctgccca atcactgttg 14461 ctgtcgcagc accttgaatt ccccagccaa atacaaacat gaacaaccaa tccaggaaca 14521 cattcaatat agcaccggta acattacaaa tcatagaata tgtggggcta ccgtcagccc 14581 gaatcaaact gctgcttgca gtagtaaaga gctggaaagg cagtcctatg gctgtaatgc 14641 cgagataggt catagccagg ggaagtacat tctccgtggc accacaaaga agaagaattt 14701 gttttttcag aacaaataca atacagaaaa ttaacagtcc gacggtaggc atcagtgtaa 14761 tacctgttcc gacaaaatgc tttgcctcct tctctctttt cgcgcccata ttaatattga 14821 agtttgctgc agttccaact ccaatcagct gggcaaaagc cgttgtaaag gttacaacag 14881 gaaaggctac actggtagct ccattgccaa gcataccaac caaacgaccg atgaaaatct 14941 gatctgtaat attataggct gctgcaacca gcatgctgat aatagacgga atggcaaact 15001 tgccaattaa tccaagtaca ggtgcataac cgagtggatt gccacttgaa cttgtatttg 15061 cagatatgtc tttcattctt gttcctcatt tccctgatat atcatttctt taaaaatttt 15121 attcatattc tcttccatct gcagcaataa agaataaagc ctgtccagat cttcctcttt 15181 tatatttcgg gttaaaagct tgttaaactt catcaattcc gttcttactt cctgcccgat 15241 ttgttttgcc ttctcagtcg gataaacccg gttttgcctt ttatcctttt catcttgtat 15301 tcgcaaaaga tagccttttt cttccaaaga tcttacagcc cgcgcagtag ctgctttgtc 15361 aacacaaaca atggaggtta attcctcctg ggttattccc tcacggtttt gcagagccat 15421 aaaaaacggc tcttctgctg ccgtaagatt gtattgactg agaaccttgc caagatacat 15481 ctgacttttt cttgccaaaa gattaattaa tcttcctata cttttattca atctcatgac 15541 ctccaaaaga attatattga aaaatggttg agctgtcaac taattttctt tcctatgttg 15601 ttggatatcc atgtgtaaaa tagaggattc aacgcaataa aatacaaaag cggacatatt 15661 atctttctgt aacatgtccg cccataaagc tttaaattat ggaatacatt tctgtctgtt 15721 caggctattt tgcacatctt tgcatttttt cttccggata attttcccgc attcaaaata 15781 ccggcttacc tcttattata aaacagacaa ccgtcccctg ttttatgttt tattcacccg 15841 agcataaccc aaaaatactc gggatctaaa ccccaggact tactctgtct atcatatttc 15901 cagtatgtta ccgtatagcc aagccggtca gcatgagaag ttccggctat atcgttttgt 15961 gcttgcagct tatatggagc aataccatcg gttttaatgg gatatagatg gggttgatag 16021 ataacgaacc cttccagggg gctaataagt ttaccttcgc tgttatagac cattccttcg 16081 taaaattcct ttctgtcact tacatccacc atataagact ggttaagctt gctgctttca 16141 acaagcactc tgtaattgtt catatattta acttcaagct tcagggtttg cataagatcc 16201 gcagattgct caggaccaag catatattcg gcattgttat tgtagaacga tataacataa 16261 taatacgtca gtgctccgct tcctccaaca gagaggctta ccattatctc cttggagcta 16321 aaaccggtaa agctgcctaa aaacagccac gcatcgaagg ccatactgta atcaggatac 16381 agaggaacaa cataccaatg cagagtactg ccgtcctgta tcattaatct aatgttttca 16441 taaaaattgt tttggttttt atcgcctaca agataaacta tatccggtac tccgtcaccg 16501 tttacatctc ctatttgcct gtcaatggtg taagtattct gtctattggc ataattaaac 16561 ctgttatccg tataattctc ataataatta atccatggat tgctataata attttgcata 16621 ttgacctccc attatattct ttattgcatt atatgaaggt caacacatta agaaaccaga 16681 taacaggttt cattataaat ttagtcctcc caaatatttc acaggatcaa taaaccatat 16741 aatagttgtt acatatccgg ctacaacagc aatataccat aagattgcag ctaatttatt 16801 aaattcgctt aatccattat cttcaatcct ctactttcat aatcattcga taatagtcaa 16861 taattgcacc gtttttggca tttttttgcc cttcaccgca aactttaaaa ccacatttct 16921 catagcatcg tatcgctctc aagttattcc ttcgaacatt tagccttaca tatctaaaag 16981 gcatcctttg ttttgaaatc cgtatcgctt ctttaatcgc gtagcttccg atacctctac 17041 ccctgctatt ctttccggaa ataaaaatgc caagtatcac agtatcaggc tcttgtgcat 17101 ctctttcaag ccatatggag ccaatgcaat ttgtgtcttc ttcaatgata aaccagcaca 17161 ctttcgaagt atcataatta gctacactgc tgttatacgg acaaaacctg ctcaaataat 17221 cagtcaaatc caaatcactc tgcaaagctt gatacatgct ttcatcagag cctgcaacat 17281 ctcttaaaac aatcatcaaa tccgtccttt tgaatgtatt tgcaacatta ttttaactta 17341 tttttattca aaaatgtaga acaaagtttc cggcctgttg gaaaatattt acttagcatt 17401 attgtacacc tgagtaaaat tgcctccttt atgtgtagtt ttccttacaa aatatttatt 17461 gcgccagtct atggaatttc aaacgggaat ctgataaaat tgatttagat aaaaattatc 17521 attcacatga aaggagtatc aaaaaagaag aaattccaaa acacacaatt cagtatccaa 17581 gcggaacatt acaaaggcaa acccatcaaa agatggcgac gcaaagctaa aggggctaaa 17641 gcattatgcc atgccagcca gttgcaatgc aataatactt acccttttag taaaaagagg 17701 gtaagttttt tattttgcct taacgttgca actgtattga agcacaccat tttataataa 17761 tttacaaagg aagtggaaat atgaaaaaac atttgctaat catcgtatca gcactcgttt 17821 cagtactgtc attgaacctc attgcagtaa cggcagctga gcccacagtc aaaaacattt 17881 actatgacga tgtgtattcc ttttcagaag gaatgtccag agtggttaaa aacggcaagt 17941 atggattcat ggatcaaaca ggtaacgttg ccatcaacct ggaatacgat tatgtaagag 18001 atttttccga cgggtttgcc gcagtttcaa aaggcggtac atggtatgac gaagaaggtt 18061 atgttgatgg aaaatgggga tttattgaca gtaccggcaa agtcgttgtc ccaattattt 18121 atgacaaagt gtgtgatttc agtgaaggtc ttgccgccgt tgtaaaagac ggtaagctgg 18181 gatttgttga cacaaccggt aaagttgtta tccctcttac atacgactgt tcaatgtatg 18241 aagaatttta tttcaaccat ggtcttgcag ttgtagcagc cggtgacttt gaagaccctg 18301 atatctttgt tattgatgaa aacggcaaca ctgcctttga tttcaaatac gattatgcga 18361 gattatccgg ctactctgaa gggatgttgg ctgttgctgt cggtggagat tggggtatcg 18421 gcggtccgta ttactgggcc cgctcctaca actttggcaa gtatggtttt atagacacca 18481 acggtaacga aattgttgca cctgtttacg attatgccag tgatttccgg gaaggcattg 18541 cagtggtatg caaagacggt aaatggggag ccattgatga aaacggcgat gttgttgttc 18601 caataatcta caccgatatc agttttccaa gcaatgggct tctttgtgtg tgtaatgatg 18661 agggaaaatg gggatatgtt gactacaccg ggaaagtagt tgcagatttc aagttcgatt 18721 tatgtaatac cttccgtgaa ggatatgtca ccaattccat tgacggaaaa tggggtgctc 18781 tggatactac aggtaaaact gtaatcccat tcaaatatta taatttgtgg aattttagtg 18841 aaggtcttgt cagagtcaga atggttgaag atggcaaatg gggttatatg gacgccggtg 18901 gaaatattgc aattgaccct gtttatcagg cagccactga cttttctgac ggagttgcca 18961 ttgttgtaaa agatggtaaa tttggaatca tttctaaaac atccattcct tacacggcaa 19021 ccccaacaac ttcaaccgtg cttgtgaacg gttcccctgt ggcttttgac gcttatctca 19081 tcaacggcaa caactacttt aagcttcggg atctggctta catattaagc ggtacggaca 19141 agcagtttga agtaacctgg gacgatactc ttaaagcaat aaatctgata tccggcaaac 19201 cctataccgt atccggcggt gaaatggcaa ccggcagcaa gaacactgcc accgcatacc 19261 cttcagctgc aactgttttc attaatggcg tgagagttga gttaacagct tatacaatta 19321 acggattcaa ttacttcaaa ctccgtgacc tcggtaaaga attcgacttt ggcgtaatct 19381 gggacggcac tgccaaaaca atacgtatag acacctccag cagctatagt gaataaaaac 19441 caaaataaaa aacgcatatt ccataaataa actgccccaa gagtcaaatt tttcaggggc 19501 agtttttatt tcgccaccaa tatctctcta ctcaatgcaa aataccgggt tttgtttgat 19561 aacaaggaat aatcatctaa aacaaagaac catcacatct ttactgcttc atacttgttc 19621 catttgctgc ctgacaatat cttcagtatc agccatggcc tgcagtgtca tggcaagaag 19681 tttgtcaagt tcctatccaa gctgttccgc tccccgctca atgacttctc ttgaacagcc 19741 ggcggcaaag cctttgctct tgtatttttt cttcagggat ttcagctcca tatcttttgt 19801 gctttttgaa ggacgcataa ggaccgctgc ccagataagt cccgtcaact cgtcaacagc 19861 aaagagtact ttttccattc cggagatttt aagcagtgct caagtctgcc tgtttaccac 19921 aggttacaat ctttcgccgc aatagggaca ataactatat tccaccggca cttcagttct 19981 acaatttgag cagtacttga aaggtgaata acggtcaatc ctttgcagat tttcctccct 20041 gatttctgtg gaaggatttt tctcaaattc ctttccgacc aaaggatcca actcataaag 20101 gcagccacag catgatgttt ttacaatata tctaacatcc catttaaaaa taggcagaaa 20161 aaatatatga aaatatttgt aatatttgta aacttcatac attgacaatc ttccgcatga 20221 cggacaaaca atattgttgc aattaccaat atgcttattt ttatcctgta tgccaaagat 20281 tgcgaaaaaa aacatataaa tcactcttcc catctgttga taccttatat ttataatata 20341 accattcaaa gaataataat caattaaagc ttagcgcaaa tttcaaagca aataccatac 20401 ttgcactaaa aaatctcaac cttttcaaac ggaatttaat cagacggttt aaagagtttt 20461 aatccggtat cttcccgttt atcgtaatgt cttcagatac gccttttgct catccgttat 20521 acgatagctt tcgcaggctt tttgaatcgt ctttttatgt acccagtcag aaagtctatg 20581 ctcttcaata tacggaatcg tcgcatccca ctgatttgcc aaagccgtgg cataaaacca 20641 tgcgatcatc attttcacat aatattcttc actgtcaacc ttttggggaa gctccaggta 20701 ctccggctta aaatccttat ccagataatg catcatcagc atacctattc cgaaacggca 20761 agtgtatgtt ttatcggaag caatccaatc tttgattctg ggaagaagct tgtccttgtg 20821 cttgccgaat actttcggtc tgagcgtatc gcacaccgcc cagttatcaa catacggaag 20881 aaaagcttca actgcggcaa tgcactcctc atagttcttc acattgctta gcaaaatgga 20941 atgaagcatg ttttcgtcat aatatgcatg cggcaaagta ttcagaaaat cacggcattc 21001 cggctctttt tcaaattccc tggcaatttt ccttaacacc ggaacccgaa cacctataac 21061 cctctcctgc ggaatatttg gtatcagctt tgcctgaaac agggcatact gtttgtctgc 21121 cacctcaaat aatcttttct gcactttggt cataatttat cctccgtatt ttataattaa 21181 aaattagtat taaacactgc catagcaact tactctctta taattatact tataaaatta 21241 aattataggt taaaaaatta aaagacgtga aagtatttcc ttcacgtcaa taatccatac 21301 ttagcaattc atcaatcttt aggctgttac tttaatgttt cttataccgc gccaaaaact 21361 gcctggttct ctcttcttta ggattcccaa acacttcctg cggagaaccc tgttcgacaa 21421 tcacgccgcc atccataaaa ataacacggt ttgccacatc tctggcaaaa tccatttcat 21481 gggtcacaat aatcattgtg gtgttgcgtt ccgccaaacc cttgataact ttcagcactt 21541 ccccggtaag ctcagggtct aaggcagatg tcggttcgtc aaaacacaga atgtccgggt 21601 ccaaagccag ggcgcgggca atagctacgc gctgctgctg tccgccggat atcatatggg 21661 gatagcaatc caacttatcc gacaggccaa cctgggacaa aagcattttg ccccggtcct 21721 gaatctcttt caaaatagct tttttattct gcttatagtc cgggcgttct tttgccagca 21781 gctcagccgc caatgtaacg tttttcagaa ccgtatactg gggaaaaaga ttaaaatttt 21841 gaaaaaccag gccgaaatgc agccgttttt tgcgcacttc gctttcccgt tgagtggaag 21901 ggtccattgc atcaaaaatt acctcattat ttacgatgat acttccttta tcgggccgct 21961 ccaggaaatt aaggcaacgc agcaatgtag tctttccgct tccggaagga cctataatcg 22021 caattacctc tcctttttcc aatgaaaaat caatcttttt caaaaccttg acggttccaa 22081 aacttttttc aatacccttt acttctaaaa atgccatgga atccccttct ttcaaacacg 22141 gaaataacca agtttctttt ccacccaatt aagcaggatg gtcaaacccc cgacaaaaac 22201 aaggaaatat atgcctgtgg caaaaagagg ccaaataagc cctttattgc tgtactctcc 22261 ggccatcata ataatctcct tatttgcaat gatgcgggca agagatgtat ccttcaccag 22321 ggtaatcact tcattgccaa ttggaggaac aatacgtttt attacttgga gcagggttac 22381 atggaagaaa atctgtgtct ttgtcattcc caaaacctgt cccgcttcat attgccctac 22441 cggaatgctt tcaataccgc cccggtagat ctccgaaaaa taacaggcat agttgataac 22501 aaaagcaacg gttgctgcaa caaaacgtcc tgattcaccg ctcggccagg aaaaaggcat 22561 atttaaaaga cccgggccaa aaaaaattat tataagctgc agcatcaggg gcgttccccg 22621 gattacccag acaaaagtcc gcaccaacca ccgaagagga taaaaacggc tcatcgaacc 22681 aaaagcaatt atcatcccca aaggcagtgc aaacaaaagc gttaatataa aaagtttgca 22741 gttgagtata aaactctcgg acagtcgacc gacaattaca agaacctcat tcatatccct 22801 tttacctcaa tatacaagcg gatagacgaa agacctgcct tccgccatcc gctctttttt 22861 tatgtattat acactaatta ttcagccagc tccagtccgt atttttcagc caatgcagcc 22921 attgtaccgt ccgccaaaaa atcgtcgaaa atcttgttga cttccgcaca aatatcagaa 22981 cccttgcgga aagcaacccc ataattttct tctgccaggt aatctacaat cacgatatcc 23041 tcatagctgg tgccttcacc ggtcattgtt tttgccaaag tcaaatccaa tacggctgcg 23101 tcggcggtac cggatttcaa ttccatcaga cagtctgtct gcaaagtctt tggaacatag 23161 tttgccttct ttaaattctc atcagccatg atggtttttt ctccggcaga actctgctcg 23221 gccaccacgg tctttcctat caaggattcc gttccgttat actcagtacc ttccttcacc 23281 aaaaccacct gagcgttctt cacataaggc tgtgtaattt ccattttcgc cttgcgatcg 23341 tcatcaattg tcaatccatt ccaaatacag tcaatggatt ttgcattcag ttccacttct 23401 ttggtatccc agttaatttc aacaaattca ggttcaactc ccagtttctc acacgttaga 23461 attgccaact ctgtgtcaaa gccggtaaaa acgccgtttt catctgtata attcatagga 23521 gcataatttg tgtagccaat gataagtttg cctttacttt taacgtaatt caaatcactt 23581 tcatcctgtg atttaccggt ttcttttgcg ccacaggcag ttaatgcaag aatcatggtc 23641 agtgtcaata gcaatgaaat taattttttc atttttaatt ttttcatttt aacttccccc 23701 aatttcaatc aaaaaatact ttatcattat atcatgttaa cataataaag caaagtcctt 23761 tttatctgat atattttttg taattttact ccaatttttt atgtttattc ataaatatat 23821 aatcagacag gttggctgat ataaaaaaca tataggcaaa accggtatat ttcataaaat 23881 aaagtttcat tccggagtaa tgttaagtaa ctctctcata ttatctttag tatcagcgtt 23941 aacttcaata tccacaattt tttttaatgt cgaaaacaaa tcaggtgaaa tggtttgcaa 24001 gtcttgattc agtatcctgc accattcctt cataacaata catgcaccgt ttcgttctct 24061 tataacaggt gacttaagtg cagtccgtac caacttttca cccattttag gatagttttt 24121 aagctcctca agcacaaaaa ccatacacaa atgttcctct gtcaaacttt ctgcaaatat 24181 aaaatcgccc attcctgtcg ccatctcgtc aaggggtagg atttcttcat atatttttgt 24241 taattcatta gcatattcag gatttttata aacaatagaa agataaccac aacgtttaac 24301 aggatttcgc ttaacagcct taaaaatcag ttccgatata tccatattca gtctctttgc 24361 tgcatcatat gcatagaaaa accgttcgtc atcagagctg ttaagaattt ttaaaatcat 24421 ttccctccac gactctttat taataatatt gccacacatc tttagcaatt catctctgcc 24481 tgctatttgg gcatttatca gccaatcctg caaattaaga atgcgccaaa attgtgttat 24541 tgttactgca tgttcagatg caatctgcaa ataaagccgc aacgcttctt cagcatattc 24601 ataaacgcta attccttcaa caggtccctc atctaaaaga gcatcaatta taacactgat 24661 accttcaaaa agttcgtcat ctatagaacc gtggcgtaat gcaccaatca aattaccttt 24721 attgccacat tccagtccaa gataagcatc cataatttca tttgcacatc ctttgcgcaa 24781 aatccattcc cggatttcat cagaggtagg ttccaaacgc tccactgcat ggattttgcc 24841 ccacccatca actttctgcg caattctaaa cacaatatca ttcccatttg ggtattttaa 24901 aactgctaca attacgtaaa gtgtaaactc ttcatacaat gcaagaacaa gaagtttgtc 24961 aataatctta tcatcattac ttagatcaaa caaaccgagc aaacctatgc ccaatttgac 25021 gagctcctcg tccgtagact gaaaagcaaa ctggcaagca tagtcaaaca aattttcaat 25081 atcaaacttt ttttgttctt ttcgaataga atccagaatg ggatcgataa ctgccaaagt 25141 gccgcattca ttcagaagtt ccgcaatttc attttgtgag ctgatactac cctttttaca 25201 gtcacttttt aaaagtttta ctattttctt agatactttt tccgaacttt ttgctccaaa 25261 gtgaaatata cctattccgt cttttgctcc gggcataaac ttaagttcat ttggaaaagt 25321 ctttttaaat ggaagagtaa aatcttttgg aagcttccct tccgaattta taccttcata 25381 aataacactg tatatacttt gtgcactcat tgaaaatctc ccttcaattt accgattatt 25441 gtttattcaa ttgatcgatc tcttgttcaa atatttacct tttcatatgt atgaacaatt 25501 aagcgtaaag gttttgctga agaaaattat aaaaaaataa aatgaaatcc atgttaccaa 25561 taaacaccat tattcttatt actacaatga tatcacaatt ctctaatttt ttccaccaat 25621 gtatagacct gatttccatt tcagcaaaga tatgttcagc attagccaaa tctttgctcc 25681 ctctttaatt ttgaccggct gtatattcct tcagtaaagt ctccacatgc cctaaaaact 25741 taaaatggat atttcagcac ttgcctgcaa agaataaatg ttgttatgga ttgcatatat 25801 tctggattat cggagggagc ttttatgagc atagagcaac aaaaaatttt tgccaaaccg 25861 ccccagtcat attggatggc ttctacccct aaagccaatt atccaaccct ggaagaagac 25921 ataaaagttg atgttgcaat tatcggaggg ggtatcaccg gtatcgccac ttcctacatg 25981 cttggcaaag ccggcgtaaa agtggctgtt attgaagccg accgcatttt acaaggcaca 26041 accggccata ccacggcaaa aataacatcc cagcatgacc tgatatacag taaaatatac 26101 agccaaatgg gcagggaatt ggcacagcaa tatgccgatg caaacgaatc tgccattcgg 26161 atgattgaaa aaatagcaac tgaaaatggc atcgaatgcg atttcgttcc ccaatccgca 26221 tatgtgtata caatgcaaga caagtatatc gacaaaataa aagatgaagc cgtgattgcc 26281 gaatccctcg gtataaaagc cacatacctt gaagaaatac ctttgccctt cccaattaaa 26341 gccgcggtcc gctttgacaa ccaggcccag ttccatcccc gaaaatttct gctgcgccta 26401 gcagaggaaa ttgttaaaag cggcaatcaa atattcgagc aaagcagaat tgtggacatt 26461 gaagatgata acaactatgt tttaattaca aatcaaggca aaaaggtaac tgcggaaaag 26521 cttattatcg cttcccatta cccatgttac aataaagccg ggctatactt tacaagacta 26581 tatccggaac ggtcatatgt tgttgccata aaagcaaaag aaagttatcc cggcggaatg 26641 tatataaaca tggaagagcc aaagcgctca ctccgcagcc aaaggtcaga tgacggcgaa 26701 ctgatactgg tcggcggtga aagtcacaaa accggacaag gtgaggatac aatcaagcat 26761 tatgaagcgt tgatagatta tgccactaaa acttttacgg tagaagatat tccttaccgg 26821 tggtccaccc aggattgcat gaccttggac ggactgcctt atgtggggca tttcacatca 26881 aacactccaa atatgtacat cgcaaccggt tacggcaagt ggggaatgac caacagcata 26941 gcttccgcga tgatattaag ggatttgata attgagggaa aaagtccctg gcaggatgtt 27001 tacaacccgt cacgcaaaac agtgttggcc tccgctaaaa actttattgt tgaaaatctt 27061 aacgtcgcag aaaaactaat tgaaggaaaa atcttgccca tggcggacaa taccgatatt 27121 aaagccggag aaggaaaaat tatcaacgtg aacggtcaga gactcggagc atacagagac 27181 caacaaggta ctctgcacgt cgtagacaca acatgtacgc atatgggttg tgaattatac 27241 tggaactctg ccgaaaaatc ctgggattgt ccctgccatg gctcaaggtt tacctatgag 27301 ggcgatataa ttgaaggacc ggcagttacg cctttaaatg tacaccgtga tgtgaacaca 27361 attgaaaaac tttttaaaga caa //