LOCUS NZ_ACVX01000047 17018 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00053, whole genome shotgun sequence. ACCESSION NZ_ACVX01000047 NZ_ACVX00000000 VERSION NZ_ACVX01000047.1 GI:256005892 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000047. FEATURES Location/Qualifiers source 1..17018 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene complement(<1..1827) /locus_tag="ClothDRAFT_2700" CDS complement(<1..1827) /locus_tag="ClothDRAFT_2700" /inference="protein motif:PFAM:PF00963" /note="PFAM: cellulosome anchoring protein cohesin region; type 3a cellulose-binding domain protein; KEGG: eca:ECA1981 endoglucanase V" /codon_start=1 /transl_table=11 /product="cellulosome anchoring protein cohesin region" /protein_id="ZP_05430839.1" /db_xref="GI:256005893" /db_xref="InterPro:IPR001956" /db_xref="InterPro:IPR002102" /translation="MRKVISMLLVVAMLTTIFAAMIPQTVSAATMTVEIGKVTAAVGS KVEIPITLKGVPSKGMANCDFVLGYDPNVLEVTEVKPGSIIKDPDPSKSFDSAIYPDR KMIVFLFAEDSGRGTYAITQDGVFATIVATVKSAAAAPITLLEVGAFADNDLVEISTT FVAGGVNLGSSVPTTQPNVPSDGVVVEIGKVTGSVGTTVEIPVYFRGVPSKGIANCDF VFRYDPNVLEIIGIDPGDIIVDPNPTKSFDTAIYPDRKIIVFLFAEDSGTGAYAITKD GVFAKIRATVKSSAPGYITFDEVGGFADNDLVEQKVSFIDGGVNVGNATPTKGATPTN TATPTKSATATPTRPSVPTNTPTNTPANTPVSGNLKVEFYNSNPSDTTNSINPQFKVT NTGSSAIDLSKLTLRYYYTVDGQKDQTFWCDHAAIIGSNGSYNGITSNVKGTFVKMSS STNNADTYLEISFTGGTLEPGAHVQIQGRFAKNDWSNYTQSNDYSFKSASQFVEWDQV TAYLNGVLVWGKEPGGSVVPSTQPVTTPPATTKPPATTIPPSDDPNAIKIKVDTVNAK PGDTVNIPVRFSGIPSKGIANCDFVYSYDPNVLEIIEIKPGEL" sig_peptide complement(1741..1827) /locus_tag="ClothDRAFT_2700" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.993 at residue 29" misc_feature complement(1330..1737) /locus_tag="ClothDRAFT_2700" /note="Type I cohesin domain, interaction partner of dockerin; Region: Type_I_cohesin_like; cd08548" /db_xref="CDD:176100" misc_feature complement(order(1360..1362,1366..1368,1378..1380, 1492..1500,1510..1512,1537..1539,1543..1545,1636..1641)) /locus_tag="ClothDRAFT_2700" /note="dockerin binding interface; other site" /db_xref="CDD:176100" misc_feature complement(871..1278) /locus_tag="ClothDRAFT_2700" /note="Type I cohesin domain, interaction partner of dockerin; Region: Type_I_cohesin_like; cd08548" /db_xref="CDD:176100" misc_feature complement(order(901..903,907..909,919..921,1030..1035, 1039..1041,1051..1053,1078..1080,1084..1086,1177..1182)) /locus_tag="ClothDRAFT_2700" /note="dockerin binding interface; other site" /db_xref="CDD:176100" misc_feature complement(442..717) /locus_tag="ClothDRAFT_2700" /note="Cellulose binding domain; Region: CBM_3; pfam00942" /db_xref="CDD:109977" misc_feature complement(<1..162) /locus_tag="ClothDRAFT_2700" /note="Type I cohesin domain, interaction partner of dockerin; Region: Type_I_cohesin_like; cd08548" /db_xref="CDD:176100" gene complement(2758..4050) /locus_tag="ClothDRAFT_2701" CDS complement(2758..4050) /locus_tag="ClothDRAFT_2701" /inference="protein motif:PFAM:PF04055" /note="PFAM: Radical SAM domain protein; KEGG: dal:Dalk_4338 radical SAM domain protein" /codon_start=1 /transl_table=11 /product="Radical SAM domain protein" /protein_id="ZP_05430840.1" /db_xref="GI:256005894" /db_xref="InterPro:IPR007197" /translation="MNVNIINMSAIVKMIVSSSIVKKTWQIHKLFSENEEYSLKNLIR SIHHLLKYERILQHQNTFVISSFIPPINTKAFGSLFDGIPGSGGNLFENFVKGKRKFP VWINIDITSRYPNNKYLDGDSGFSERHFNDMEREKLISLINEIQDMGVGIIGLAGGEP LLRNDLEEIVENIDDRSVSYVYSTGYGLTSQRARDLKSAGLYGIIIDFQSMNEGEHDE KMGFNGAYHYAVNAIENSKKAELYTVSRTFCNRELLEKETMLEFIKFLGQCGVDEVRL MEPMPFGKSGRISRDELFTDDERKKLIELHMLCNKDANLPKVSVCSYFESREMFGCGA AGYYSFIDIHGDLYPCDYIPFNFGNVFETPIKALWKKMYKSFETPEVHCRSLICLGKM ERDKFQQYPQRVKTLPESCKTCCGQVTPGFYKILGGEK" misc_feature complement(3133..3687) /locus_tag="ClothDRAFT_2701" /note="Radical SAM superfamily. Enzymes of this family generate radicals by combining a 4Fe-4S cluster and S-adenosylmethionine (SAM) in close proximity. They are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster...; Region: Radical_SAM; cd01335" /db_xref="CDD:100105" misc_feature complement(order(3205..3210,3307..3309,3430..3432, 3499..3507,3574..3579,3583..3585)) /locus_tag="ClothDRAFT_2701" /note="FeS/SAM binding site; other site" /db_xref="CDD:100105" gene complement(4244..5818) /locus_tag="ClothDRAFT_2702" CDS complement(4244..5818) /locus_tag="ClothDRAFT_2702" /inference="protein motif:PFAM:PF00092" /note="PFAM: von Willebrand factor type A; SMART: von Willebrand factor type A; KEGG: dal:Dalk_0318 von Willebrand factor type A" /codon_start=1 /transl_table=11 /product="von Willebrand factor type A" /protein_id="ZP_05430841.1" /db_xref="GI:256005895" /db_xref="InterPro:IPR002016" /db_xref="InterPro:IPR002035" /translation="MAFDSVFLMAVLFSSCSSSVNKGLNSSLMVENSHLEKGTGYYDI IDMDINIGDDDTYSLEYNTEDYNVIEENIFLDTYNNPLSTFSVDVDTASYSNIRRFLN SSQKPPVDAVRIEEMINYFTYDYPDADGDEPFSITTEIGQCPWNPENKLMLVGLQTKK LSTEQLPPSNLVFLIDVSGSMDEPNKLPLLKSAFKLLVDELDEDDRVSIVVYAGAAGL VLDSTPGNEKDKILDALMNLEAGGSTAGAEGIKLAYDVAKKNFIKSGNNRVILATDGD FNVGISSEAELVRLIEKKRDEGIFLTVLGFGTGNYKDSKMESLADKGNGNYAYIDNIA EARKVLVNEMGATLNTVAKDVKIQVEFNPAKVKAYRLIGYENRLLRNEDFNNDSVDAG EIGAGHSVTALYEIVSANSDFEVSKVDELKYQKSQLVESDEIATVKVRYKKPDSDTSE LLSETVLEKAEENTSKNLEFAAAVAEFGMLLRESKYKGNSSYDHVLEVAKQYADGDDG YRSEFVKLVEKAESIN" sig_peptide complement(5750..5818) /locus_tag="ClothDRAFT_2702" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.937) with cleavage site probability 0.444 at residue 23" misc_feature complement(5363..5650) /locus_tag="ClothDRAFT_2702" /note="von Willebrand factor; Region: vWF_A; pfam12450" /db_xref="CDD:152884" misc_feature complement(4268..5389) /locus_tag="ClothDRAFT_2702" /note="Uncharacterized protein containing a von Willebrand factor type A (vWA) domain [General function prediction only]; Region: COG2304" /db_xref="CDD:32459" misc_feature complement(4799..5314) /locus_tag="ClothDRAFT_2702" /note="VWA subgroup: Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-...; Region: vWA_subgroup; cd01465" /db_xref="CDD:29238" misc_feature complement(order(4997..4999,5075..5077,5279..5281, 5285..5287,5291..5293)) /locus_tag="ClothDRAFT_2702" /note="metal ion-dependent adhesion site (MIDAS); other site" /db_xref="CDD:29238" misc_feature complement(4247..4783) /locus_tag="ClothDRAFT_2702" /note="Domain of unknown function (DUF3520); Region: DUF3520; pfam12034" /db_xref="CDD:152469" gene complement(5825..6745) /locus_tag="ClothDRAFT_2703" CDS complement(5825..6745) /locus_tag="ClothDRAFT_2703" /inference="protein motif:TFAM:TIGR00099" /note="TIGRFAM: Cof-like hydrolase; HAD-superfamily hydrolase, subfamily IIB; PFAM: Haloacid dehalogenase domain protein hydrolase type 3; sucrose-6F-phosphate phosphohydrolase; Haloacid dehalogenase domain protein hydrolase; KEGG: asu:Asuc_0324 Cof-like hydrolase" /codon_start=1 /transl_table=11 /product="Cof-like hydrolase" /protein_id="ZP_05430842.1" /db_xref="GI:256005896" /db_xref="InterPro:IPR000150" /db_xref="InterPro:IPR005834" /db_xref="InterPro:IPR006379" /db_xref="InterPro:IPR006380" /db_xref="InterPro:IPR013200" /translation="MEKKFEGLMLVSDLDGTLLNSKLEVSEENIKAVSYFVDNGGIFT IATGRMELGTRKYLEVLPVNAPVILYNGALIYDFNKEERIWTCGFRQDIRNLLKELLD RFPYLGIEIFPGGDNVYLLRENEETEKHSKKEGFKPVVISVDEMPKNFYKIILTANPD RLPEVEEFLKPMAQGFRTVYSEKQFLEILDNETSKGRALAELAKIMGIEKDNVISVGD NQNDLEMIKVSGTGFAVENAHPELIEACDFVCVHHDRHAASYVIDWIEKNIVNKNKTW NQSLETPSNTKNIKRRRELYENEYCFKIQK" misc_feature complement(5960..6718) /locus_tag="ClothDRAFT_2703" /note="haloacid dehalogenase-like hydrolase; Region: Hydrolase_3; pfam08282" /db_xref="CDD:116866" misc_feature complement(6431..6718) /locus_tag="ClothDRAFT_2703" /note="Haloacid dehalogenase-like hydrolases. The haloacid dehalogenase-like (HAD) superfamily includes L-2-haloacid dehalogenase, epoxide hydrolase, phosphoserine phosphatase, phosphomannomutase, phosphoglycolate phosphatase, P-type ATPase, and many others...; Region: HAD_like; cd01427" /db_xref="CDD:119389" misc_feature complement(order(6602..6607,6701..6709)) /locus_tag="ClothDRAFT_2703" /note="active site" /db_xref="CDD:119389" misc_feature complement(6692..6709) /locus_tag="ClothDRAFT_2703" /note="motif I; other site" /db_xref="CDD:119389" misc_feature complement(6605..6607) /locus_tag="ClothDRAFT_2703" /note="motif II; other site" /db_xref="CDD:119389" misc_feature complement(5960..>6304) /locus_tag="ClothDRAFT_2703" /note="Haloacid dehalogenase-like hydrolases. The haloacid dehalogenase-like (HAD) superfamily includes L-2-haloacid dehalogenase, epoxide hydrolase, phosphoserine phosphatase, phosphomannomutase, phosphoglycolate phosphatase, P-type ATPase, and many others...; Region: HAD_like; cl11391" /db_xref="CDD:187016" gene complement(6761..7432) /locus_tag="ClothDRAFT_2704" CDS complement(6761..7432) /locus_tag="ClothDRAFT_2704" /inference="protein motif:TFAM:TIGR01509" /note="TIGRFAM: HAD-superfamily hydrolase, subfamily IA, variant 3; HAD-superfamily hydrolase, subfamily IA, variant 1; PFAM: Haloacid dehalogenase domain protein hydrolase; KEGG: asu:Asuc_0616 HAD family hydrolase" /codon_start=1 /transl_table=11 /product="HAD-superfamily hydrolase, subfamily IA, variant 3" /protein_id="ZP_05430843.1" /db_xref="GI:256005897" /db_xref="InterPro:IPR005833" /db_xref="InterPro:IPR005834" /db_xref="InterPro:IPR006402" /db_xref="InterPro:IPR006439" /translation="MKKVKAVIFDMDGLMIDTERLYFEVERIMARKFGKEVKDETLWK MMGRKPLEAITVFAEDLELDISPKKLLEIRDELFVKKLVNEVEPMPGLFDILNILKGK VKMAIATGSPQKFLKIVLDKLKIESYFDVFVTSDEVEKGKPDPEVYNTAVKRLKVAPF ECVVLEDSSNGALAAVRAGCYTIAVPTVYTNKQDFSFVNYVAKDLKDAAEKINEFLCS QEIEF" misc_feature complement(6785..7426) /locus_tag="ClothDRAFT_2704" /note="Predicted phosphatase/phosphohexomutase [General function prediction only]; Region: COG0637" /db_xref="CDD:30982" misc_feature complement(<7046..7417) /locus_tag="ClothDRAFT_2704" /note="Haloacid dehalogenase-like hydrolases. The haloacid dehalogenase-like (HAD) superfamily includes L-2-haloacid dehalogenase, epoxide hydrolase, phosphoserine phosphatase, phosphomannomutase, phosphoglycolate phosphatase, P-type ATPase, and many others...; Region: HAD_like; cl11391" /db_xref="CDD:187016" misc_feature complement(6878..7180) /locus_tag="ClothDRAFT_2704" /note="Haloacid dehalogenase-like hydrolases. The haloacid dehalogenase-like (HAD) superfamily includes L-2-haloacid dehalogenase, epoxide hydrolase, phosphoserine phosphatase, phosphomannomutase, phosphoglycolate phosphatase, P-type ATPase, and many others...; Region: HAD_like; cd01427" /db_xref="CDD:119389" misc_feature complement(7106..7108) /locus_tag="ClothDRAFT_2704" /note="motif II; other site" /db_xref="CDD:119389" gene 7599..8360 /locus_tag="ClothDRAFT_2705" CDS 7599..8360 /locus_tag="ClothDRAFT_2705" /inference="protein motif:PFAM:PF01643" /note="PFAM: acyl-ACP thioesterase; KEGG: geo:Geob_2623 acyl-ACP thioesterase" /codon_start=1 /transl_table=11 /product="acyl-ACP thioesterase" /protein_id="ZP_05430844.1" /db_xref="GI:256005898" /db_xref="InterPro:IPR002864" /translation="MQKKRFSKKYEVHYYEINSMQEATLLSLLNYMEDCAISHSTSAG YGVNELLAADAGWVLYRWLIKIDRLPKLGETITVQTWASSFERFYGNREFIVLDGRDN PIVKASSVWIYFNIKKRKPMRIPLEMGDAYGIDETRALEEPFTDFDFDFEPKVIEEFT VKRSDIDTNSHVNNKKYVDWIMETVPQQIYDNYKVTSLQIIYKKESSLGSGIKAGCVI DEQNTDNPRLLHKIWDKNTGLELVSAETIWQKIQS" misc_feature 7614..8348 /locus_tag="ClothDRAFT_2705" /note="Acyl-ACP thioesterase; Region: Acyl-ACP_TE; pfam01643" /db_xref="CDD:145011" misc_feature 7614..7943 /locus_tag="ClothDRAFT_2705" /note="4-hydroxybenzoyl-CoA thioesterase (4HBT). Catalyzes the final step in the 4-chlorobenzoate degradation pathway in which 4-chlorobenzoate is converted to 4-hydroxybenzoate in certain soil-dwelling bacteria. 4HBT forms a homotetramer with four active...; Region: 4HBT; cd00586" /db_xref="CDD:48031" misc_feature order(7695..7697,7767..7769,7773..7775,7854..7865) /locus_tag="ClothDRAFT_2705" /note="active site" /db_xref="CDD:48031" misc_feature 8070..8342 /locus_tag="ClothDRAFT_2705" /note="The hotdog fold was initially identified in the E. coli FabA (beta-hydroxydecanoyl-acyl carrier protein (ACP)-dehydratase) structure and subsequently in 4HBT (4-hydroxybenzoyl-CoA thioesterase) from Pseudomonas. A number of other seemingly unrelated...; Region: hot_dog; cl00509" /db_xref="CDD:186045" misc_feature order(8115..8120,8202..8213) /locus_tag="ClothDRAFT_2705" /note="active site 2" /db_xref="CDD:48035" misc_feature order(8133..8135,8142..8147,8154..8156,8181..8186) /locus_tag="ClothDRAFT_2705" /note="active site 1" /db_xref="CDD:48035" gene complement(8827..9003) /locus_tag="ClothDRAFT_2706" CDS complement(8827..9003) /locus_tag="ClothDRAFT_2706" /inference="protein motif:PFAM:PF07561" /note="PFAM: protein of unknown function DUF1540" /codon_start=1 /transl_table=11 /product="protein of unknown function DUF1540" /protein_id="ZP_05430845.1" /db_xref="GI:256005899" /db_xref="InterPro:IPR011437" /translation="MKVQKMNHPNEGIKCVVNTCHYYMNGDYCAAEKVEIQPRNAMDS NQTDCATFVPNVEG" misc_feature complement(8848..8967) /locus_tag="ClothDRAFT_2706" /note="Domain of Unknown Function (DUF1540); Region: DUF1540; pfam07561" /db_xref="CDD:148913" gene complement(9300..9890) /locus_tag="ClothDRAFT_2707" CDS complement(9300..9890) /locus_tag="ClothDRAFT_2707" /inference="protein motif:TFAM:TIGR02841" /note="TIGRFAM: sporulation protein YyaC; PFAM: protein of unknown function DUF1256" /codon_start=1 /transl_table=11 /product="sporulation protein YyaC" /protein_id="ZP_05430846.1" /db_xref="GI:256005900" /db_xref="InterPro:IPR009665" /translation="MLKTKKMYFSTDEKLCQYRLNQEMYNMVKNVGKQYKRIAVVGIG TDRSTGDSFGPLVGYMLSKCKIYDFDVYGTIVEPVHALNLKETMDKIDTSNTLVIAVD ASIGSIEHIGYIGLCNEPIKPGSGVGKDLPPVGDISVSGIVAFGGFAPYMVLQNTSLG LVYKMAEITSNAIKYVLYKQQLEPQRNSKLSAFAAV" misc_feature complement(9372..9785) /locus_tag="ClothDRAFT_2707" /note="Protein of unknown function (DUF1256); Region: DUF1256; cl06087" /db_xref="CDD:141909" gene complement(10039..10731) /locus_tag="ClothDRAFT_2708" CDS complement(10039..10731) /locus_tag="ClothDRAFT_2708" /inference="protein motif:PFAM:PF00072" /note="PFAM: response regulator receiver; transcriptional regulator domain protein; SMART: response regulator receiver; KEGG: reh:H16_A1818 response regulator, OmpR-family" /codon_start=1 /transl_table=11 /product="two component transcriptional regulator, winged helix family" /protein_id="ZP_05430847.1" /db_xref="GI:256005901" /db_xref="InterPro:IPR001789" /db_xref="InterPro:IPR001867" /translation="MRKILIVEDDESIGELLRDYLEINGFSVDICMNGTEGLKRIRDG EYDLLILDIMLPGMNGYEILKKIRDEKDIPVLIVSAKKEEFDKIHGLKLGADDYITKP FSPGELVARVNAHIAKYERLRNKYGNNNVNSITIRGLEIQKDSRRVFVNGKEVNLAQK EFDVLLFLAQNPNRVFSREEIFDRVWGMDALGDAATVTVHIGRIREKIESDPSNPQYI ETVWGAGYRLRA" misc_feature complement(10042..10725) /locus_tag="ClothDRAFT_2708" /note="Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription]; Region: OmpR; COG0745" /db_xref="CDD:31088" misc_feature complement(10384..10719) /locus_tag="ClothDRAFT_2708" /note="Signal receiver domain; originally thought to be unique to bacteria (CheY, OmpR, NtrC, and PhoB), now recently identified in eukaroytes ETR1 Arabidopsis thaliana; this domain receives the signal from the sensor partner in a two-component systems...; Region: REC; cd00156" /db_xref="CDD:29071" misc_feature complement(order(10426..10431,10438..10440,10495..10497, 10552..10554,10576..10578,10705..10710)) /locus_tag="ClothDRAFT_2708" /note="active site" /db_xref="CDD:29071" misc_feature complement(10576..10578) /locus_tag="ClothDRAFT_2708" /note="phosphorylation site" /db_xref="CDD:29071" misc_feature complement(order(10552..10560,10564..10569)) /locus_tag="ClothDRAFT_2708" /note="intermolecular recognition site; other site" /db_xref="CDD:29071" misc_feature complement(10423..10431) /locus_tag="ClothDRAFT_2708" /note="dimerization interface; other site" /db_xref="CDD:29071" misc_feature complement(10048..10332) /locus_tag="ClothDRAFT_2708" /note="Effector domain of response regulator. Bacteria and certain eukaryotes like protozoa and higher plants use two-component signal transduction systems to detect and respond to changes in the environment. The system consists of a sensor histidine kinase...; Region: trans_reg_C; cd00383" /db_xref="CDD:29475" misc_feature complement(order(10057..10059,10072..10074,10108..10113, 10135..10137,10144..10146,10198..10203,10258..10260)) /locus_tag="ClothDRAFT_2708" /note="DNA binding site" /db_xref="CDD:29475" gene complement(10749..12188) /locus_tag="ClothDRAFT_2709" CDS complement(10749..12188) /locus_tag="ClothDRAFT_2709" /inference="protein motif:PFAM:PF02518" /note="PFAM: ATP-binding region ATPase domain protein; histidine kinase HAMP region domain protein; histidine kinase A domain protein; SMART: ATP-binding region ATPase domain protein; histidine kinase A domain protein; histidine kinase HAMP region domain protein; KEGG: sde:Sde_2723 sensor histidine kinase" /codon_start=1 /transl_table=11 /product="histidine kinase" /protein_id="ZP_05430848.1" /db_xref="GI:256005902" /db_xref="InterPro:IPR003594" /db_xref="InterPro:IPR003660" /db_xref="InterPro:IPR003661" /db_xref="InterPro:IPR004358" /db_xref="InterPro:IPR005467" /translation="MDLKKRLIFSNAAIIVIPLGITFVASFIFMFVLARIHDVDLSYN NVKKLTQIQYEFFKAEGGLLKNSPEIILEKDFQQYITTRLESIEADIVVLKGQERVFE TRKLSIIELERCLEKTGDNLFRNIVEIQGKSHMVKVIPVIFKSGEDGKILLLVPAVND WMTTEKLFIFSGVVFVLSFIITNIVIITAFSKKVITPLGKLQAAAGKISEGNLDFEII EDGDTQIRELCRSFEKMRLKLVEANYTQKKYDESRKMLFSSISHDLKTPITSIKGYVE GILDGVANTPQKVEKYLRTVHSKAVHMDRMIDDLLLYSRLDMHKVSFNFEKTDVLKYF EDCMYEIDIELEKSNIKVELHNNLRGKRYIMIDRDQVRRVVINIIDNSRKYMDKEQGK IDIFLREATSNVVIEIKDNGAGISESDLSYIFDRFYRADSARDTRKGSGLGLAIAKQI IEGHGGKIWAVSRMGEGTSVMISLKKYEG" misc_feature complement(10761..11744) /locus_tag="ClothDRAFT_2709" /note="Signal transduction histidine kinase [Signal transduction mechanisms]; Region: BaeS; COG0642" /db_xref="CDD:30987" misc_feature complement(11466..11678) /locus_tag="ClothDRAFT_2709" /note="Methyl-accepting protein, and Phosphatase (HAMP) domain. HAMP is a signaling domain which occurs in a wide variety of signaling proteins, many of which are bacterial. The HAMP domain consists of two alpha helices connected by an extended linker. The...; Region: HAMP; cl01054" /db_xref="CDD:154171" misc_feature complement(11244..11441) /locus_tag="ClothDRAFT_2709" /note="Histidine Kinase A (dimerization/phosphoacceptor) domain; Histidine Kinase A dimers are formed through parallel association of 2 domains creating 4-helix bundles; usually these domains contain a conserved His residue and are activated via trans-...; Region: HisKA; cd00082" /db_xref="CDD:119399" misc_feature complement(order(11259..11261,11271..11273,11280..11282, 11292..11294,11301..11303,11313..11315,11367..11369, 11376..11378,11388..11390,11397..11399,11409..11411, 11421..11423)) /locus_tag="ClothDRAFT_2709" /note="dimer interface; other site" /db_xref="CDD:119399" misc_feature complement(11403..11405) /locus_tag="ClothDRAFT_2709" /note="phosphorylation site" /db_xref="CDD:119399" misc_feature complement(10767..11078) /locus_tag="ClothDRAFT_2709" /note="Histidine kinase-like ATPases; This family includes several ATP-binding proteins for example: histidine kinase, DNA gyrase B, topoisomerases, heat shock protein HSP90, phytochrome-like ATPases and DNA mismatch repair proteins; Region: HATPase_c; cd00075" /db_xref="CDD:28956" misc_feature complement(order(10779..10781,10785..10790,10803..10805, 10809..10811,10857..10868,10944..10949,10953..10955, 10959..10961,10965..10967,11037..11039,11046..11048, 11058..11060)) /locus_tag="ClothDRAFT_2709" /note="ATP binding site; other site" /db_xref="CDD:28956" misc_feature complement(11046..11048) /locus_tag="ClothDRAFT_2709" /note="Mg2+ binding site; other site" /db_xref="CDD:28956" misc_feature complement(order(10860..10862,10866..10868,10947..10949, 10953..10955)) /locus_tag="ClothDRAFT_2709" /note="G-X-G motif; other site" /db_xref="CDD:28956" gene complement(12219..12971) /locus_tag="ClothDRAFT_2710" CDS complement(12219..12971) /locus_tag="ClothDRAFT_2710" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: cco:CCC13826_1721 ABC transporter" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430849.1" /db_xref="GI:256005903" /translation="MKAFKAAFINEVEKIYRKKKVVVAAILSILAIVMGQLVVTGINR GFGLIAASSTQFPILVLSLLVNTILPLFTTLVAIDVFSGEFSHNTMKITLTRPVSRMK LYAAKICAVAFFVLANLLMIMLLSLVAGFIFNAVSLSVSGVLRIVLSYIVTLIPIMCF ALIVVFFSNVLRSGTGVFFLSIVLFIAFNVLSVIFPKYSNLFITSMFDWYVLWNVDII PLDKLIREFLIMAGYAIMFYTAGYYMFDKRDL" sig_peptide complement(12864..12971) /locus_tag="ClothDRAFT_2710" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.712) with cleavage site probability 0.339 at residue 36" gene complement(12958..13683) /locus_tag="ClothDRAFT_2711" CDS complement(12958..13683) /locus_tag="ClothDRAFT_2711" /inference="protein motif:PFAM:PF00005" /note="PFAM: ABC transporter related; SMART: AAA ATPase; KEGG: ade:Adeh_0636 ABC transporter, ATPase subunit" /codon_start=1 /transl_table=11 /product="ABC transporter related protein" /protein_id="ZP_05430850.1" /db_xref="GI:256005904" /db_xref="InterPro:IPR003439" /db_xref="InterPro:IPR003593" /db_xref="InterPro:IPR017871" /translation="METVLEIKGLTKLYKNGRGIFDINLEVYRGDVFGFLGPNGAGKT TAMKIMTGLMHPNSGDVKIFGHSVITEFEQAMKKVGCIIETAESYAYLTPYENLKLFA RFYDDVDDKRIDEVLELTGILKFKKEKVKNFSLGMKQRLGLAAAILSRPELVILDEPL NGLDVEGMIEIRKLIKALAENEKTTFFISSHLIHDVEITCNRIGILYDGKLVNVDTTE NILKNFASLENYFVSEVGKNESV" misc_feature complement(13048..13671) /locus_tag="ClothDRAFT_2711" /note="P-loop containing Nucleoside Triphosphate Hydrolases; Region: P-loop NTPase; cl09099" /db_xref="CDD:158411" misc_feature complement(13051..13671) /locus_tag="ClothDRAFT_2711" /note="gallidermin-class lantibiotic protection ABC transporter, ATP-binding subunit; Region: galliderm_ABC; TIGR03740" /db_xref="CDD:163452" misc_feature complement(13552..13575) /locus_tag="ClothDRAFT_2711" /note="Walker A/P-loop; other site" /db_xref="CDD:72971" misc_feature complement(order(13111..13113,13210..13215,13432..13434, 13549..13557,13561..13566)) /locus_tag="ClothDRAFT_2711" /note="ATP binding site; other site" /db_xref="CDD:72971" misc_feature complement(13432..13443) /locus_tag="ClothDRAFT_2711" /note="Q-loop/lid; other site" /db_xref="CDD:72971" misc_feature complement(13258..13287) /locus_tag="ClothDRAFT_2711" /note="ABC transporter signature motif; other site" /db_xref="CDD:72971" misc_feature complement(13210..13227) /locus_tag="ClothDRAFT_2711" /note="Walker B; other site" /db_xref="CDD:72971" misc_feature complement(13192..13203) /locus_tag="ClothDRAFT_2711" /note="D-loop; other site" /db_xref="CDD:72971" misc_feature complement(13105..13125) /locus_tag="ClothDRAFT_2711" /note="H-loop/switch region; other site" /db_xref="CDD:72971" gene complement(13784..15025) /locus_tag="ClothDRAFT_2712" CDS complement(13784..15025) /locus_tag="ClothDRAFT_2712" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430851.1" /db_xref="GI:256005905" /translation="MGKKKNLTMLISFCIGAVLFVSTAFADVVSKTGYEQFKDAIKNT VGSLAEEYDSFTTETGLSVKDNDRILAANYAIEKYDMVNSRREEVSTLEWGGIKTSNY YSYRDKWCNIYSVDEDTYYVNEYETEMKDAVRINNVFEDEDFEYIERIFDALIGNLKE YVVVTEDADGNKEFSGKLSDAQIPAVVNAVIAYAAKKTVFDESQRQNGIDLPVIKNDV FVKEVRGRANLNGDGIIENLFGEIIVSGSEENGTKHDFKIELVFKVYNINSTVVEKPD LTGKKVEKTVVQNGYNSNALQKYMGKWKNDIVIEESDRFVKIGERIIEITSFDSDYVY GTYTEIYKDEYADYGSSNSYSLKANINEERGRLEITDASGNVTEGYMYLEMGRIDFYI PSEKPEKDVYNPIFNKVFDEE" sig_peptide complement(14945..15025) /locus_tag="ClothDRAFT_2712" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.999 at residue 27" gene complement(15248..16855) /locus_tag="ClothDRAFT_2713" CDS complement(15248..16855) /locus_tag="ClothDRAFT_2713" /inference="protein motif:PFAM:PF01943" /note="PFAM: polysaccharide biosynthesis protein; virulence factor MVIN family protein; KEGG: glo:Glov_2048 integral membrane protein MviN" /codon_start=1 /transl_table=11 /product="polysaccharide biosynthesis protein" /protein_id="ZP_05430852.1" /db_xref="GI:256005906" /db_xref="InterPro:IPR002797" /db_xref="InterPro:IPR004268" /translation="MKKQSITKGFAVLSAAGLITKILSVLYIPFLLAIIGDEGNGIYA AAYQVYVFIYVIANSGIPVAIAKSVSELTAVGNYKDALRIFKISRFFLIIIGTVLTVL MFVTAKPLAVMINSEKSFLAIAALSPTLFFTALASAYKGYFQGMSNMTPTAVSQVVEQ IFNMIFTVLFAALLINKSLEAACAGGTVGTTVGALASVIVLIFIYNRRREEINNLKEH RKTAKRYSYKQLATRIFYYSLPITVCVAAQYAGNLIDVANIRGRLLAGGYTLEMASVM HSYLSKYQQIMNAPISIVSALAAAVLPSISGAAAEQDIKQVKDKSNHAFRLCMLIVIP SAVGLSILSEPIYAVLKYGAGSHLMRYGSIVLVLMSIVQIQSSILQGAGKLYKATINV ILGIIAKIIFNYILIANPNINIMGAVIGSIVGYGLTIILNVMTVRKELKIKINILKQA VKPAVSSVVMGIFVWIVYKGLYFVLGFIKSAYLVNALSTVVSVLFGMAIYFYIMILVR GITKNDFDVLPEKIRRMIPKFVLNKAV" misc_feature complement(15938..16840) /locus_tag="ClothDRAFT_2713" /note="Polysaccharide biosynthesis protein; Region: Polysacc_synt; cl10513" /db_xref="CDD:187004" misc_feature complement(15353..16834) /locus_tag="ClothDRAFT_2713" /note="stage V sporulation protein B; Region: spore_V_B; TIGR02900" /db_xref="CDD:163065" ORIGIN 1 caattctccc ggttttatct ctattatctc aagtacattc gggtcatagc tgtatacaaa 61 gtcacagttt gctattccct tggatggtat accactgaat cttacaggta tatttactgt 121 gtctcccggt tttgcattta ctgtgtccac cttaatcttt attgcattcg gatcatctga 181 cggcggtatt gttgttgcag gtggttttgt tgttgcaggt ggtgttgtta caggctgtgt 241 tgatggtact acactgccac cgggttcttt accccataca agaacaccgt tcaagtatgc 301 tgttacctga tcccattcaa caaactgtga agcagacttg aatgagtagt catttgactg 361 tgtatagtta ctccagtcat tctttgcaaa tctaccttgt atctgaacat gtgcacccgg 421 ttcaagagtt ccgcctgtaa agcttatttc aaggtaggtg tctgcgttat ttgttgagga 481 actcattttt acaaatgttc cttttacatt tgaagtaatt ccgttgtagc tgccgttact 541 gccgattatt gcagcatggt cacaccagaa ggtctgatct ttctgtccgt ctactgtata 601 ataatatctc aatgtgagtt tggacaaatc aattgcactg cttccggtat tagtaacctt 661 gaactgagga ttgattgagt tagtagtatc tgaaggattg ctgttgtaga attcaacctt 721 caaattgcct gataccggtg tatttgccgg tgtgtttgtc ggtgtgtttg tcggtaccga 781 tggcctggtg ggcgtagccg tagctgattt tgtcggcgta gctgtatttg ttggtgttgc 841 tcccttggtc ggtgttgcat tgccaacgtt aacaccaccg tctataaatg ataccttctg 901 ttctaccagg tcattatctg caaatccacc tacttcgtcg aaagtaatat agcccggagc 961 acttgatttt acagttgctc ttatttttgc aaatactccg tctttagtta ttgcatacgc 1021 tcctgttccg ctgtcttccg caaacaggaa tactattatc tttctgtcag gatatattgc 1081 agtatcaaag ctcttggtag gattcgggtc aactattatg tctccgggat ctatccctat 1141 aatttccaat acattcggat catatctgaa cacaaagtcg cagtttgcta ttcctttgga 1201 tggaactcct ctgaaatata caggtatttc aactgtagtt ccaacagatc ccgtaacttt 1261 gccaatttct actaccacac cgtctgacgg aacatttggc tgtgttgtcg gtacggaact 1321 accaagattt actccgcccg cgacaaaagt tgtgcttatt tctactaaat cgttgtccgc 1381 aaatgcacct acttcaagca aagtaatcgg tgccgctgca gctgatttga cagtggctac 1441 aattgttgca aatactccat cctgagttat tgcatacgtt cctcttccac tgtcttctgc 1501 aaacagaaat acaatcatct ttcgatccgg atatattgcg ctatcaaagc tcttgctagg 1561 atccggatct tttattatgc ttcctggttt tacttctgtt acttccagca catttggatc 1621 ataacccaat acgaagtcgc aattggccat tcctttggat ggcactcctt tcagggttat 1681 aggtatttct acttttgatc caacggctgc tgtaactttg ccgatctcga ctgtcattgt 1741 ggccgccgat actgtctgcg gtatcatcgc cgcaaaaatc gtcgtcagca tagccacaac 1801 taagagcata ctgatgactt ttctcatcta ccattcctcc caaaaattaa tattttatat 1861 gatcttataa aaagatctct aaaggcattt gaaatcaaat accttatata caaaccaagc 1921 aagacaataa caaaaattag tgttaatatt aataaacaat attaatattt tcttgtccga 1981 ttttcagttt taataaatac cagagaattt gtcgataaat attggggagt ggtttttaat 2041 aaatcgatgg aacatacgat tctatgtata tgctttgata tttgcacaat caatacatat 2101 aaagtattga atacgctatt tcattgaaat gctgggaatt aataaacgct ctccagtatg 2161 gttatcttat gctcaaagaa ccaaaacata aacagacgtg accaatccct tgtacaaact 2221 cctccctcta aaacttttta tctatataaa ctctgtatct tgggatatgt tagaggccat 2281 taaaacagga tttaaataaa atattaatta ctgctacatg aactgaatac tgagatatta 2341 agcgcttaat ttaatgaaga tttgcctcca aactcccaaa tacataagtt attaacaatt 2401 ctctctctaa caatgaatct gtaataaaaa ttaaccgtgt tttaatttca ttatatatta 2461 aaattaaatt actttcaata agttttcaaa ataaagccca cctgttttca aactctatgt 2521 acagtctaaa accgtacttc tgataaaaaa ccgtctgcca acatatttgc ggcacctcaa 2581 gctccatccc cccgacagct taaatcaaac atactaacaa aattactatt tgcaatgaaa 2641 ttattactct ttttatctgg gcatcttctt tcttgtaata tatattcgat atataaacgg 2701 aatttgaggg gcattttttt aaataatatt ttttcatttg aaagtattat tacaaaatta 2761 cttttctccc ccaagtattt tgtaaaatcc cggtgtgact tgcccgcagc acgttttgca 2821 actttccggc aaagttttca ccctttgcgg atattgttga aatttatccc gttccatttt 2881 cccaagacaa attagcgaac ggcaatgcac ttccggcgtt tcaaaacttt tatacatctt 2941 tttccataaa gcctttatcg gagtttcaaa aacattgcca aaattaaagg gtatgtaatc 3001 gcacggatat aaatcaccat gtatatcaat aaatgaatag tatcccgcag caccgcatcc 3061 gaacatctcc cgggattcaa agtaagagca aacggatacc ttcggcaaat tcgcatcttt 3121 gttgcataac atatgcaatt caatcagttt ctttcgttca tcatcagtaa acaattcatc 3181 ccggcttatt cttccgcttt ttccaaaagg catgggctcc atcaatctta cttcatcaac 3241 tccacactgt ccaagaaatt ttataaactc tagcattgtt tccttttcta aaagctccct 3301 gttgcagaaa gtccttgaaa cagtatagag ctcggctttt tttgagtttt caattgcatt 3361 tacggcataa tgataagctc cgttaaagcc cattttttca tcatgttcgc cttcattcat 3421 tgattgaaaa tctattatta tgccatacaa gcctgccgat tttaaatccc tcgctctttg 3481 agaggtcaaa ccgtatcctg ttgaatagac ataagaaaca cttctgtcat caatattttc 3541 aacgatttct tcaaggtcat ttctaagtaa aggttctcct ccggcaagtc ctataattcc 3601 aacacccatg tcctgtatct catttatcaa agatatgagt ttttctcttt ccatatcatt 3661 aaaatgtctt tccgaaaaac cgctatcacc gtctaaatat ttgttgttcg gatatcttga 3721 cgtaatatca atatttatcc aaacgggaaa cttccttttt cctttcacaa agttttcaaa 3781 aagattcccg ccgcttccag gtatgccgtc aaacaagctg ccaaacgctt tggtatttat 3841 cggcggaata aaagagctta tcacaaacgt attctgatgc tgcaaaattc tttcgtactt 3901 taataaatgg tgtatgcttc ttattaaatt tttcagactg tattcctcat tctccgaaaa 3961 caatttatga atttgccatg tttttttaac aatcgatgaa ctcacaatca tttttacaat 4021 tgctgacata ttaattatat taacattcat aaaaaaagtc atccctcata tagaattgtt 4081 aaagcagtta aaacaacttt acaattatat tctatcaaaa cattttactt tttcaaatca 4141 atatactttt ttaaaaattc ttgtccatga aaaaactgcc tctaattggc tctcgaggca 4201 gtttttcata ttgattgttt tcacattatt ttttatatca atatcaatta atactttccg 4261 ctttttccac cagctttaca aattcactcc tgtatccgtc gtcaccgtca gcatattgct 4321 ttgccacctc taaaacatga tcataagaag aattgccttt atactttgat tctctgagca 4381 acatgccgaa ttctgccaca gctgccgcaa attccaggtt ttttgatgta ttttcttctg 4441 ctttttccaa cacagtttct gaaagcagtt cacttgtatc cgagtcaggc tttttatatc 4501 ttaccttcac agtggcaatt tcatcgcttt ctacaagctg agatttttga tacttgagct 4561 cgtcaacttt cgaaacctca aaatccgaat tcgcagaaac aatctcataa agtgcggtta 4621 ccgagtgtcc cgcacctatt tctcccgcgt ccaccgaatc gttgttaaaa tcctcatttc 4681 taagaagcct gttttcatac ccaatcagcc tgtaagcctt tacctttgcg ggattgaatt 4741 caacctgaat ttttacatcc tttgcaaccg tgtttaaagt tgcacccatt tcatttacaa 4801 gaactttcct ggcttccgca atattgtcta tgtaggcata gtttccgttt cctttgtctg 4861 caaggctttc catctttgaa tccttgtaat tgcccgttcc aaaaccaagc actgtaagga 4921 atataccttc atctcttttt ttctcaatca gcctgacaag ctcagcctcg ctgcttatac 4981 caacattaaa atctccgtcc gttgccagaa taacacgatt gttgcctgac ttaataaagt 5041 ttttcttagc aacgtcatag gcaagtttta ttccttcggc tcctgccgtc gagcctccgg 5101 cttcaagatt catcagagcg tcaagaattt tgtccttttc atttcccgga gtcgaatcca 5161 aaaccaaacc tgccgcaccc gcataaacta caatagacac tctgtcatct tcgtccagtt 5221 cgtccacaag aagcttaaaa gctgatttta aaaggggaag tttgttaggt tcatccattg 5281 aacccgaaac atctatcaga aaaaccaggt tgcttggagg cagctgttcc gtggagagct 5341 tttttgtctg aagtcctaca agcataagct tgttttccgg attccacggg cactgtccaa 5401 tctcggtggt tatgctgaac ggttcatcgc cgtcggcatc aggatagtca tatgtaaaat 5461 agttaatcat ttcttcaatt cttacggcat ccacaggagg tttttgcgaa gaattgagaa 5521 aacgccttat attgctgtag gatgccgtat ccacgtccac agaaaatgtt gaaaggggat 5581 tgttgtaggt atcaagaaat atattttcct caataacatt atagtcttcg gtattatatt 5641 ccaaactata ggtatcatcg tcaccaatat taatatccat gtcaattatg tcataatatc 5701 cggtaccttt ttccaaatgt gaattctcaa ccattaatga tgaatttaaa cctttgttga 5761 cagaactgct gcaggaagaa aacaacaccg ccattaaaaa aacggaatca aaagccataa 5821 tacattactt ctgtattttg aaacaatatt cattttcata caattccctc cgtcttttta 5881 tgttttttgt attagacgga gtttctaaag attggttcca cgttttgttt ttattcacaa 5941 tatttttttc aatccagtca atcacataag aagcggcatg tctgtcatga tggacgcaaa 6001 caaagtcaca agcttctatc aattccggat gcgcgttttc aacggcaaac cctgttcccg 6061 acacctttat catttctaaa tcattttgat tgtcaccaac agaaatcaca ttatcttttt 6121 caattcccat tatttttgca agctcggcca aagccctacc ttttgaagtt tcattgtcaa 6181 gaatttctaa aaactgtttt tctgaataca ccgtcctgaa tccttgagcc ataggcttta 6241 aaaattcctc cacttcggga agcctgtccg ggttagctgt aagtataatc ttgtaaaagt 6301 ttttgggcat ttcatccaca gaaatcacca caggtttgaa tccttctttt ttactgtgtt 6361 tttcggtctc ttcattctcc cgcagcaaat aaacattgtc ccctcccggg aatatttcaa 6421 tcccaagata aggaaaccta tccaaaagct ctttcaacaa attccttata tcctgtctga 6481 aaccacaggt ccaaattctt tcttctttat taaaatcata tataagggca ccgttgtata 6541 aaatcacggg cgcatttacc ggcaagacct ccaaatattt tcttgtccca agctccatcc 6601 tgcccgtcgc aatggtaaat attcctccat tgtccacaaa atatgaaacg gcttttatat 6661 tttcctcact tacttccaac ttgctgttta gcaaagtccc gtccaaatca ctgacaagca 6721 tcaaaccttc aaactttttc tccatcacag aactatcctt tcaaaactca atttcttgac 6781 tgcaaagaaa ttcattgatt ttttccgcag catcctttaa atctttggca acataattta 6841 caaaactaaa atcctgtttg tttgtataca ccgtaggtac ggcaatggta taacatccgg 6901 ccctaaccgc cgcaagagca ccgttgctcg aatcctcaag aacaacacac tcaaaaggtg 6961 ccactttaag cctctttacc gcagtattgt aaacttcggg atcgggcttt cccttttcaa 7021 cctcatccga tgtcacaaaa acatcaaaat agctttctat cttaagctta tccagcacaa 7081 ttttcagaaa tttttgagga gaacctgtgg ctatagccat cttcaccttt cctttaagga 7141 tgttcagaat atcaaaaagc cccggcatcg gttcaacttc atttacaagc tttttgacaa 7201 acagctcatc cctaatctcc aaaagcttct ttggtgaaat atcaagttcc agatcctctg 7261 caaaaacagt gatggcttcc aatggctttc ttcccatcat cttccacagg gtttcatcct 7321 ttacctcttt accaaatttt cgagccatta ttctttccac ttcaaaatac agtcgctccg 7381 tatcaatcat caatccatcc atatcaaaaa taaccgcttt aacttttttc aaacaagaac 7441 accacccatt ttttataaaa caaccattaa aattgagaaa tatgaataaa ttttaacctc 7501 agaaacattt ttttacatca aactgctttg tctttccctg acacgtataa taatataata 7561 taatagtaaa caataataat ttcaagggaa tgatgtgaat gcagaagaaa agattttcaa 7621 agaagtatga agtacattac tacgaaatca actcaatgca ggaagcaact cttctctccc 7681 tgctaaacta tatggaggac tgcgcaatat cccactcaac ctctgccgga tacggtgtca 7741 acgagttatt ggctgctgac gcaggatggg tattataccg ctggttaatt aaaatagaca 7801 gacttcccaa gctcggagaa acaattactg ttcagacatg ggcctcttcc ttcgaacgct 7861 tctacggcaa cagggaattt atcgtattgg acggcaggga taaccccatt gtcaaagcct 7921 catccgtatg gatatatttc aatattaaaa aaagaaaacc tatgagaatc cccctcgaaa 7981 tgggagatgc ttatggcata gacgaaacaa gagctttgga agaacccttt accgacttcg 8041 attttgattt tgaacccaaa gttattgaag aatttactgt aaaaagaagt gatatagaca 8101 caaacagcca cgtaaacaac aagaaatacg ttgactggat tatggaaacc gtaccccagc 8161 aaatatatga caactacaaa gttacatctc ttcagattat atacaaaaag gaatcttctt 8221 tgggttcagg cataaaggcc ggatgtgtaa ttgatgagca aaataccgat aatccgcggc 8281 tccttcacaa aatatgggac aagaataccg gtttggagct tgtatccgcc gaaacaatct 8341 ggcaaaagat tcagtcataa taaatataca taaaaaacat ataatgtgca ggtcagttca 8401 caaactttaa atcaatgcca aaaaagggtt gagtaccgtt ttcgtactca acccttgtat 8461 agttaggtaa gacacttaag gaaaatcatt aaagggggcg aacaattttt attttgatct 8521 ttttttcgct catttattct ttttgttatg taacgtacct attatgtatt tattaaaatc 8581 gaacatatgt tcgataaata tcttactact tttcataaaa attctctaat tataatttaa 8641 ccccatatag ctcattgaaa caaatttctt acatattaac tcatgctatc tcaaaatttc 8701 ttgttttttt gttacctatt gtgtttgcaa ataaaaattt ttgagcctgc acggcgaggt 8761 ccaacaacct tgccggcagg caacaaatta taaataaact tacgttattc ttggactaaa 8821 tttccattat ccctcaacat tcggaacaaa cgttgcacag tctgtctggt tggaatccat 8881 tgcatttctc ggctgaattt caactttctc cgcagcgcag tagtcaccgt tcatataata 8941 atggcacgtg tttacaacac actttattcc ctcattgggg tgattcatct tctgaacctt 9001 cataacgctt cctcccggat ttgtagttgt agttaacaaa ctacaatatt atttttaaaa 9061 gaatacactg ttttaataca tccaattaat atgaaaatca taaatatcag aaatgaacac 9121 aaagtaataa ccgtaaaaat tcacgcataa aaaaagcaca gtttcttcta tttatatatt 9181 tatagaaata tataatagat attctgtgca aaaagccata cttttcttaa attttccgta 9241 attttacttt gtatttttat gacaattata atacaaacat agacaaacaa ttaaattttt 9301 tacacagcgg caaaagcact taattttgaa ttcctttgag gctccagctg ctgtttataa 9361 agaacatact ttatagcatt gctggttatc tcagccattt tataaaccag cccaaggctt 9421 gtattctgaa gcaccatata aggcgcaaat cctccaaatg ccacaatacc ggacaccgat 9481 atatcgccga cgggaggtaa atccttgccc actccgcttc cgggctttat cggttcattg 9541 cacagcccga tatatccgat atgctcaatg cttcctatag acgcatcaac ggcaattaca 9601 agagtattcg acgtatctat tttgtccatt gtctccttga gatttaacgc atgtacgggt 9661 tcgacaatcg taccgtaaac atcaaaatca tatattttgc actttgaaag catatagcca 9721 accaaaggtc caaaactgtc tcctgtcgaa cgatccgtac ctatacctac aactgcaatc 9781 cttttatatt gcttaccgac gttttttacc atgttgtaca tttcctggtt cagcctatac 9841 tgacacagtt tctcatcagt gctgaaatac attttcttcg tttttaacat accatctact 9901 cccctaaaca aaatacaaca attttaaaca aacaagccgc ttattttcta tttattcagc 9961 tttacaagta cattatacaa taaatacaat tcataaaaaa gtaaaattat tcacatctta 10021 ttgctaagat tttcttcttt atgctctcag tctataccct gctccccaga cagtttcaat 10081 atattgggga tttgaagggt cagactcgat tttctccctt attcttccaa tatgaacagt 10141 cactgttgcc gcatctccca aagcatccat tccccatact ctgtcaaaaa tctcttccct 10201 tgaaaaaact ctgttgggat tctgggccaa aaacaaaaga acgtcaaatt ctttttgtgc 10261 cagatttact tcttttccgt tcacaaaaac ccttctggaa tccttttgaa tttcaagtcc 10321 tcttatagta atggaattta cattattatt cccatatttg tttctgagtc tctcgtattt 10381 tgctatgtga gcattcaccc ttgcaacaag ctcccccgga ctgaaaggtt ttgtaatata 10441 gtcatcggct ccaagtttca aaccgtgaat tttgtcaaat tcttctttct ttgcagacac 10501 aataagcaca ggaatatctt tttcatcccg gatttttttc aatatttcat atccattcat 10561 accgggcagc ataatgtcaa gtatcaaaag atcgtactcc ccgtctctga tacgctttaa 10621 accttcagtc ccattcatgc atatgtccac agaaaagccg ttgatttcaa gatagtcccg 10681 caaaagctct cctatgcttt catcatcttc aacaataaga atctttctca tcacatcata 10741 cctccctttc agccttcata ttttttcaga gaaatcatca cactcgtgcc ttcgcccata 10801 cggctgaccg cccaaatttt ccctccgtgt ccttctatta tttgtttggc aatggcaagt 10861 ccaagcccgc ttcctttcct ggtatccctc gccgaatcgg cgcgatagaa cctgtcaaaa 10921 atgtaggaca aatcactttc actaattccg gctccgttgt cttttatctc tattaccaca 10981 tttgatgttg cttccctcaa aaaaatatct attttcccct gttccttgtc catatatttt 11041 cttgagttgt caattatgtt tatcacaact cttcgcacct gatccctgtc tatcattata 11101 taacgctttc ctctcaagtt gttatgaagc tcaaccttga tattggactt ttcaagttca 11161 atatctattt catacatgca atcttcaaag tactttagca catccgtctt ttcaaaatta 11221 aacgaaacct tgtgcatatc cagtctcgaa tacaaaagaa ggtcatcaat cattctatcc 11281 atgtgaacag ccttggaatg aaccgttctt aaatattttt ccactttctg aggggtattt 11341 gccacaccgt ccaatatccc ctcaacatat ccctttattg aagttatagg agttttaaga 11401 tcgtgagata tgcttgaaaa aagcattttt ctgctctcat catatttttt ctgcgtataa 11461 tttgcctcca caagcttaag cctcattttc tcaaaggagc ggcacaattc tctaatttgg 11521 gtatctccgt cctcaataat ctcaaaatcc agattgcctt cgcttatttt gcccgcagca 11581 gcctgaagct tccccagagg agttataact ttctttgaaa aagcagtaat gatgactata 11641 tttgttatta taaagctgag aacaaacacc acgccggaaa atatgaaaag cttttccgtt 11701 gtcatccagt catttacggc gggcacaagc aaaagaattt tcccatcctc accgctttta 11761 aatatcacag gtattacttt taccatatgg gatttgccct gaatctcaac aatgtttcta 11821 aacaagttgt cgccggtttt ttcaagacat ctttccaact caataatact gagcttgcga 11881 gtttcaaaaa cccgttcctg gccttttagc accactatgt cggcttctat gctctcaagt 11941 cttgtggtaa tatactgctg aaaatctttt tccaaaatta tttccgggga atttttaagc 12001 aatcccccct ctgctttaaa aaactcgtat tgtatctgag taagcttttt tacattattg 12061 taacttaggt cgacatcatg tatcctcgcc aaaacaaaca taaaaataaa agatgccaca 12121 aatgttatcc caagaggaat aacaataatt gcagcattcg aaaaaataag gcgttttttt 12181 aaatccatgt ctcatttcac ctttttataa aatctctttt ataagtctct cttgtcaaac 12241 atataatagc ctgcggtata aaacattata gcatatcctg ccataataag gaactctcgt 12301 ataagtttgt ccaacggtat gatgtcaaca ttccacaaaa cataccagtc aaacattgat 12361 gtaataaaaa ggtttgaata ctttggaaaa atgaccgaaa gcacattaaa cgctatgaat 12421 aaaacaattg ataaaaagaa cacccctgtt ccgcttctta aaacatttga gaaaaacaca 12481 actataagag caaagcacat tataggtatc aaagtaacaa tataggacaa aactattctt 12541 aaaactccgg ataccgataa cgatacggcg ttaaaaataa atcccgccac taaagacagc 12601 agcattatca tcaacagatt tgcaagaacg aaaaaagcca cggcacaaat cttagccgca 12661 tacagtttca tcctcgaaac cggtctggtt aatgtaatct tcatggtgtt gtgagaaaac 12721 tcaccggaaa atacatctat ggcaacaagt gttgtaaaca acggcaatat ggtgttaacc 12781 aacaaagaca gcaccaggat cggaaactgt gtactgctgg cagctatcaa tccaaaaccc 12841 ctgtttattc cggtaaccac cagttgtccc ataactatgg caaggattga gagtatggct 12901 gccacaacga ctttcttttt tctgtatatt ttctcgactt catttataaa cgcggcctta 12961 aacgctttca tttttaccta cctcgcttac aaaatagttc tccagtgaag caaagttttt 13021 aagtatgttt tcggttgtat ccacattaac cagttttccg tcgtacagta ttccaatcct 13081 gttacatgtt atttccacat catggataag atggcttgaa ataaagaatg ttgtcttttc 13141 gttctctgca agtgccttga taagttttct gatttctatc atgccttcca cgtcaagtcc 13201 atttaacggc tcgtccaata tcaccaattc gggccttgac aaaattgccg cagccaaccc 13261 gagcctttgc ttcataccaa gagaaaaatt cttcactttt tcttttttga acttaagaat 13321 gccggtgagc tccaggactt catcaattct tttatcatcc acgtcatcat agaatctagc 13381 aaaaagtttt aaattctcat aaggtgtaag atatgcatag gattccgcag tttcaattat 13441 acagcccact ttcttcatgg cctgttcaaa ttcggttata acgctgtgcc cgaaaatttt 13501 tacgtctccg ctgttcggat gcataagacc cgtcataatt ttcatagcgg tagtctttcc 13561 cgcaccgttg gggccgagga atccaaacac atcccctctg tatacttcaa gatttatatc 13621 aaaaattcct cttccattct tatataattt ggtaagtccc tttatttcaa gtacagtttc 13681 cattgaaccc tccgatataa acaataattt acgaaaccga cagcggaaaa gccgctgcca 13741 gtttcaaata aatccttaaa atattatccg ttccaatcta tccttattcc tcatcaaaaa 13801 ctttattgaa tataggattg tacacgtctt tctcaggttt ttcacttgga atgtagaaat 13861 caattcttcc catctccagg tacatataac cctcagttac attgcctgaa gcatcggtaa 13921 tttcaaggcg gcctctctct tcatttatat ttgccttcaa gctgtaactg ttcgagctgc 13981 cgtaatcggc atattcgtcc ttgtaaattt cggtatatgt tccataaaca tagtcgctgt 14041 caaagctcgt aatttctata attctctcac ctatcttgac gaatctgtca ctttcctcta 14101 tcacaatgtc attcttccat ttacccatat acttctgtaa agcatttgag ttatatccgt 14161 tttgtacaac agttttttct actttcttgc cggttaaatc gggtttttcc actacagtag 14221 aatttatgtt gtagaccttg aacacaagtt caattttaaa atcatgcttt gttccgtttt 14281 cctcgcttcc ggaaactata atctctccaa acaggttttc aattattccg tctccgttta 14341 aatttgccct tcctcttact tctttcacaa aaacatcatt tttaatgaca ggaaggtcaa 14401 ttccattctg tctttgcgat tcgtcaaata cagttttctt cgcggcatat gcgataactg 14461 cattaactac cgcaggaatc tgtgcatcgc ttaatttccc ggaaaattcc ttgtttccgt 14521 cagcgtcttc tgttacgaca acatactcct tgagatttcc tatcaaagca tcaaatattc 14581 tctcaatgta ctcaaaatct tcatcttcaa agacattgtt aattcttact gcgtccttca 14641 tctcggtttc atactcgttg acataatagg tatcttcatc gacgctatat atattgcacc 14701 acttatccct gtaggaataa taattgcttg tcttaattcc tccccactcc aaagttgaga 14761 cctcttccct tctgctgttg accatatcgt atttttctat tgcgtaattt gccgccagta 14821 ttctgtcatt atcttttacg gataatcccg tctccgtagt gaagctgtca tattcctctg 14881 caaggcttcc cactgtattc tttattgcat ccttaaattg ttcatacccc gttttggaaa 14941 cgacatctgc aaaagccgtt gatacaaaca ataccgctcc gatgcaaaaa cttatgagca 15001 tcgtcagatt cttctttttt cccatttgcc acctctcctt ttttgcaata tttaaggaaa 15061 cgtttcctca cttactattc tatatggcag ctataaattt caactaaata atatataaac 15121 aatttataaa ctttttttgc aatttcatat aaacttgctt cccatacagc ttatccgctt 15181 atttataaca tttcaatctt atccgtaaca tttcaataat attatttcaa taatattttt 15241 aatcagttca tacggctttg tttaatacga atttgggtat cattcttctg attttttccg 15301 gcaatacgtc aaaatcattt tttgttattc ccctgacaag tatcattata taaaaatata 15361 ttgccattcc gaacagaact gaaacaactg tagataatgc gtttacaaga tatgcgctct 15421 taataaatcc taaaacaaag tataaaccct tgtatacaat ccatacaaaa atacccatta 15481 ccactgatga aacagccggt tttaccgcct gtttcagtat atttattttt attttcaact 15541 cttttcttac tgtcataaca ttgagaataa tggtcaaacc gtatcccact atacttccta 15601 tcactgctcc catgatattt atattgggat ttgctataag tatataattg aaaattatct 15661 ttgcgataat acctaaaatt acatttatcg ttgctttgta cagttttcct gcaccctgca 15721 aaattgacga ctgtatttgt acaatggaca tgagaacgag tactattgag ccgtagcgca 15781 taaggtggga acccgctccg tatttcaata cggcgtaaat aggttcactc aatatggaca 15841 accccacagc cgacggaatt actatcagca tgcaaagcct gaaagcatgg ttggatttat 15901 ccttaacctg ctttatatct tgttccgccg cagctcccga aatggaaggc agcaccgccg 15961 ccgcaagagc cgaaactatg gaaatcggtg cgttcattat ttgctggtat ttggacaaat 16021 agctgtgcat gaccgatgcc atttccagcg tatagccgcc ggccaaaaga cgccctctta 16081 tatttgccac atcaatgaga tttccagcat attgagcagc cacacaaaca gttatgggta 16141 ggctgtaata aaatattctt gtcgcaagct gcttgtatga gtatctcttt gcagtcttcc 16201 tgtgttcctt cagattgtta atttcttctc ttcttctgtt gtatataaat ataaggacaa 16261 taacggaagc cagcgcaccc acagttgttc ctacggtacc tccggcgcac gcagcctcaa 16321 ggcttttatt tattagcaac gctgcaaaaa gcactgtgaa tatcatattg aatatctgtt 16381 caaccacctg ggacacggca gtcggagtca tgttgctcat gccctggaaa tatcccttgt 16441 acgcagaggc cagggcggta aaaaacagtg tcggagacaa tgccgctatt gcaaggaatg 16501 atttttccga gttaatcatg acagccaatg gctttgccgt gacaaacata agcactgtta 16561 aaacagtacc tattataata aggaaaaaac gcgatatttt aaagattctc aaagcatcct 16621 tgtagtttcc cacagcggta agctcagaca cggactttgc tatcgcaacg ggaatgcctg 16681 aattggcaat aacataaata aaaacatata cttgatacgc ggcagcataa ataccatttc 16741 cctcatctcc gataatggca agcaaaaaag gaatataaag caccgataat atttttgtta 16801 taagtcctgc cgccgacagt accgcaaaac cttttgtgat tgactgtttc ttcatatctg 16861 ctcccaattt ccataaatta tgtcttctac tgatattatt gacagcttgc aagccgtcat 16921 acctattcta atataatgat tggagttctt caagctcaat tgcctttaaa tttttaaaga 16981 aaggatataa aagctcggcg taccaggttc cttgaaat //