LOCUS NZ_ACVX01000075 2600 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00137, whole genome shotgun sequence. ACCESSION NZ_ACVX01000075 NZ_ACVX00000000 VERSION NZ_ACVX01000075.1 GI:256006157 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000075. FEATURES Location/Qualifiers source 1..2600 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene 384..1658 /locus_tag="ClothDRAFT_2939" CDS 384..1658 /locus_tag="ClothDRAFT_2939" /EC_number="6.3.4.4" /inference="protein motif:TFAM:TIGR00184" /note="KEGG: dal:Dalk_2837 adenylosuccinate synthase; TIGRFAM: adenylosuccinate synthetase; PFAM: adenylosuccinate synthetase; SMART: adenylosuccinate synthetase" /codon_start=1 /transl_table=11 /product="adenylosuccinate synthetase" /protein_id="ZP_05431076.1" /db_xref="GI:256006158" /db_xref="InterPro:IPR001114" /translation="MATRVVVGTQWGDEGKGKYIDMLAKDSDMVVRFSGGNNAGHTIV ANGVKYALHLIPSGILNEGKTCIIGNGVVVDPAVLLKEIKELNEKGISTDRLLISDRA HVIMPYHKLLDELQEKFRGENSIGTTKRGIGPCYSDKTERSGIRMCDLVDEDEFVRKV RENLKVKNLIIEKVYGGQKLDEEQVISEYLEYGRKLKEYVADVNSIIFEAIEQGKNIL FEGAQATFLDLDFGTYPYVTSSNPVAGGVCTGAGVGPVFINEVYGVLKAYTSRVGAGP FPTEQNNEIGDRIRELGWEYGTTTGRPRRCGWLDLVMIKYAARVNGLTALAINHVDTI GKLPKIKLCVAYKKNGQETRNFPCSLKELAQCEPVYEEFDGWDEDISNVKSFDDLPDN AKKYLSRIEEIVGVKIKLIGVGKEREQTIVVN" misc_feature 384..1655 /locus_tag="ClothDRAFT_2939" /note="adenylosuccinate synthetase; Provisional; Region: PRK01117" /db_xref="CDD:179227" misc_feature 396..1628 /locus_tag="ClothDRAFT_2939" /note="Adenylosuccinate synthetase (AdSS) catalyzes the first step in the de novo biosynthesis of AMP. IMP and L-aspartate are conjugated in a two-step reaction accompanied by the hydrolysis of GTP to GDP in the presence of Mg2+. In the first step, the r-...; Region: AdSS; cd03108" /db_xref="CDD:73337" misc_feature order(420..422,426..434,501..509,513..515,1371..1373, 1377..1379,1623..1628) /locus_tag="ClothDRAFT_2939" /note="GDP-binding site; other site" /db_xref="CDD:73337" misc_feature order(426..428,435..437,504..506) /locus_tag="ClothDRAFT_2939" /note="ACT binding site; other site" /db_xref="CDD:73337" misc_feature order(495..497,756..758,765..767,1050..1052,1095..1097) /locus_tag="ClothDRAFT_2939" /note="IMP binding site; other site" /db_xref="CDD:73337" gene 1876..1951 /locus_tag="ClothDRAFT_R0049" /note="tRNA-Lys3" tRNA 1876..1951 /locus_tag="ClothDRAFT_R0049" /product="tRNA-Lys" gene 2115..2189 /locus_tag="ClothDRAFT_R0050" /note="tRNA-Glu3" tRNA 2115..2189 /locus_tag="ClothDRAFT_R0050" /product="tRNA-Glu" gene 2278..2354 /locus_tag="ClothDRAFT_R0051" /note="tRNA-Asp2" tRNA 2278..2354 /locus_tag="ClothDRAFT_R0051" /product="tRNA-Asp" gene 2404..2479 /locus_tag="ClothDRAFT_R0052" /note="tRNA-Thr4" tRNA 2404..2479 /locus_tag="ClothDRAFT_R0052" /product="tRNA-Thr" gene 2483..2567 /locus_tag="ClothDRAFT_R0053" /note="tRNA-Tyr2" tRNA 2483..2567 /locus_tag="ClothDRAFT_R0053" /product="tRNA-Tyr" ORIGIN 1 tttaatgtct tattttttgt gccttttatc ttcatataaa ctttttggaa tttttacttt 61 gattgtcagc aaaaaccgaa ctattggcct cgaattttag ttaaatgttc gactttttgg 121 ttgtttttat ttggtttaaa tgatattata taattggtga ttgtttaaag ttgtaattga 181 atcattttga agttataatt taaatgttcc aaaaaattaa tgcagtacca ttaaagtata 241 gcggcagcga acggacttag tgtcttactg cttcaaacgt tgaccgggta taaattgctt 301 tatttttatg cttaatttcc taaatacggt ttataccggt ttattaatga tggtctgact 361 tctggaatag ggggaatata aaaatggcta ccagagttgt agtaggcacc cagtggggag 421 atgagggaaa aggcaagtat attgacatgc tggccaaaga ctcggacatg gtggtgcgat 481 tttcaggagg aaacaatgcc ggacacacga tagtggccaa cggtgtaaaa tatgcgttgc 541 atcttatacc gtcgggcata ttgaatgaag gcaaaacttg tattataggc aacggtgttg 601 tggttgatcc ggcagttttg ctaaaggaaa ttaaggagct taatgagaaa gggataagta 661 ctgacaggct tttgataagt gacagggctc atgttatcat gccgtaccac aaacttttag 721 atgagcttca ggagaagttt cgtggagaga attcaatagg gacaaccaaa agaggaattg 781 ggccgtgcta ctctgacaag acggaacgat cgggaatcag aatgtgcgac cttgttgatg 841 aagatgaatt tgtcaggaag gtaagagaaa acttgaaggt taagaacctc ataattgaaa 901 aggtatacgg cggacaaaaa ctggatgagg aacaggttat atccgaatat cttgaatatg 961 gaagaaagct taaggaatac gttgcggatg taaacagcat tatatttgag gccatagagc 1021 agggaaaaaa tatattgttt gaaggagccc aggcaacatt tttggatctt gatttcggaa 1081 cctaccctta tgtcacttct tccaatcctg tggcaggtgg agtttgtaca ggtgcaggag 1141 tcggacctgt ttttatcaat gaggtatatg gggttctgaa agcctatacg tcaagagttg 1201 gcgcaggacc gttcccgacg gaacagaaca acgaaatagg cgacagaata agagaacttg 1261 gatgggaata tggcacaact acgggaaggc caagacgctg cgggtggctt gatctcgtta 1321 tgataaagta tgctgccaga gtaaacggac ttaccgcact ggcaataaac catgttgata 1381 caataggaaa gctgccaaaa atcaagcttt gtgttgcgta taaaaagaac gggcaggaaa 1441 cgcgcaattt cccgtgcagc ttaaaagagc ttgcccaatg tgaacctgta tatgaggaat 1501 ttgacggttg ggatgaagac atatcaaacg taaagtcctt tgatgatctt cctgacaacg 1561 cgaaaaagta tctgagcaga atagaagaaa ttgtcggagt aaaaataaaa ctgattggtg 1621 tggggaagga aagagagcag actatagtcg taaactaaaa tgagctttcg atgactttgc 1681 aggcactcaa aactgctcag gttcttgagc agttttgggt agccgggaag ctaaaggaat 1741 tatatccata aaaaaattaa aaaattacac aaaaaattta aaaaaatcag ttgacagaac 1801 cctataacct attgtaatat atataactgt gtgatgcagc aaataacaga gatgctgatg 1861 ttcacgcaga tttatgggcc attagctcag ttggcagagc acatgacttt taatcatggt 1921 gtcccgcgtt cgagtcgcgg atggctcacc aggtaataaa aggaagctta tgacgcagaa 1981 ggtgtcataa gattcctttt aaaacctctt aaaaggcgga attcggaata aaaaacgaaa 2041 ttttcacttg acattttgag aggttccggc taaaatatac tgtgtcagcg aggttttaag 2101 ttgatttcag acatggccca ttggtcaagc ggttaagaca ccgccctttc acggcggtaa 2161 caggggttcg agtcccctat gggtcaccat tatcagcctg tatttggtgc tggtctgcca 2221 gaagctgttt atcgctggca gaccagtagg caggtcaaaa agaaaggcaa acaacatggc 2281 ccggtagttc agttggttag aatgccagcc tgtcacgctg gaggtcgagg gttcgagccc 2341 cttccgggtc gccactatag atttaaatta aattataata aatttcttgt aaatttaata 2401 taagctggtg tagctcagca ggtagagcag ctgacttgta atcagcaggt cgggggttcg 2461 attccgtcca ccagctccat atggagggat tcccgagtgg ccaaaggggg cagactgtaa 2521 atctgttggc tcagccttcg atggttcgaa tccatctccc tccaccagac aaaagcgagt 2581 ataaaatact cgcttttgtg //