# Copyright 2006-2013 by Peter Cock. All rights reserved. # # This file is part of the Biopython distribution and governed by your # choice of the "Biopython License Agreement" or the "BSD 3-Clause License". # Please see the LICENSE file that should have been included as part of this # package. """Bio.SeqIO support module (not for general use). Unless you are writing a new parser or writer for Bio.SeqIO, you should not use this module. It provides base classes to try and simplify things. """ from __future__ import print_function import sys # for checking if Python 2 from Bio.Alphabet import generic_alphabet from Bio.Seq import Seq, MutableSeq from Bio.SeqRecord import SeqRecord class SequenceIterator(object): """Base class for building SeqRecord iterators. You should write a __next__ method to return SeqRecord objects. You may wish to redefine the __init__ method as well. """ def __init__(self, handle, alphabet=generic_alphabet): """Create a SequenceIterator object. Arguments: - handle - input file - alphabet - optional, e.g. Bio.Alphabet.generic_protein This method MAY be overridden by any subclass, for example if you need to process a header or accept additional arguments. Note when subclassing: - there should be a single non-optional argument, the handle. - you do not have to require an alphabet. - you can add additional optional arguments. """ self.handle = handle self.alphabet = alphabet def __next__(self): """Return the next record in the file. This method's stub-implementation MUST be overridden by any subclass to actually parse the file and return the next entry as a SeqRecord object. """ raise NotImplementedError("The subclass should implement the __next__ method.") if sys.version_info[0] < 3: def next(self): """Python 2 style alias for Python 3 style __next__ method.""" return self.__next__() def __iter__(self): """Iterate over the entries as a SeqRecord objects. Example usage for Fasta files:: with open("example.fasta","r") as myFile: myFastaReader = FastaIterator(myFile) for record in myFastaReader: print(record.id) print(record.seq) This method SHOULD NOT be overridden by any subclass. It should be left as is, which will call the subclass implementation of __next__ to actually parse the file. """ return iter(self.__next__, None) # Function variant of the SequenceWriter method. def _get_seq_string(record): """Use this to catch errors like the sequence being None (PRIVATE).""" if not isinstance(record, SeqRecord): raise TypeError("Expected a SeqRecord object") if record.seq is None: raise TypeError("SeqRecord (id=%s) has None for its sequence." % record.id) elif not isinstance(record.seq, (Seq, MutableSeq)): raise TypeError("SeqRecord (id=%s) has an invalid sequence." % record.id) return str(record.seq) # Function variant of the SequenceWriter method. def _clean(text): """Use this to avoid getting newlines in the output (PRIVATE).""" return text.replace("\n", " ").replace("\r", " ") class SequenceWriter(object): """Base class for building SeqRecord writers. Interlaced file formats (e.g. Clustal) should subclass directly. Sequential file formats (e.g. Fasta, GenBank) should subclass the SequentialSequenceWriter class instead. """ def __init__(self, handle): """Create the writer object. Use the method write_file() to actually record your sequence records. """ self.handle = handle def _get_seq_string(self, record): """Use this to catch errors like the sequence being None (PRIVATE).""" if not isinstance(record, SeqRecord): raise TypeError("Expected a SeqRecord object") if record.seq is None: raise TypeError("SeqRecord (id=%s) has None for its sequence." % record.id) elif not isinstance(record.seq, (Seq, MutableSeq)): raise TypeError("SeqRecord (id=%s) has an invalid sequence." % record.id) return str(record.seq) def clean(self, text): """Use this to avoid getting newlines in the output.""" return text.replace("\n", " ").replace("\r", " ") def write_file(self, records): """Use this to write an entire file containing the given records. records - A list or iterator returning SeqRecord objects Should return the number of records (as an integer). This method can only be called once. """ # Note when implementing this, your writer class should NOT close the # file at the end, but the calling code should. raise NotImplementedError("This object should be subclassed") ##################################################### # You SHOULD subclass this # ##################################################### class SequentialSequenceWriter(SequenceWriter): """Base class for sequence writers. This class should be subclassed. It is intended for sequential file formats with an (optional) header, repeated records, and an (optional) footer. In this case (as with interlaced file formats), the user may simply call the write_file() method and be done. However, they may also call the write_header(), followed by multiple calls to write_record() and/or write_records() followed finally by write_footer(). Users must call write_header() and write_footer() even when the file format concerned doesn't have a header or footer. This is to try and make life as easy as possible when switching the output format. Note that write_header() cannot require any assumptions about the number of records. """ def __init__(self, handle): """Initialize the class.""" self.handle = handle self._header_written = False self._record_written = False self._footer_written = False def write_header(self): """Write the file header. If your file format defines a header, you should implement this method in order to write the header before any of the records. The default implementation checks the private attribute ._header_written to ensure the header is only written once. """ assert not self._header_written, "You have aleady called write_header()" assert ( not self._record_written ), "You have aleady called write_record() or write_records()" assert not self._footer_written, "You have aleady called write_footer()" self._header_written = True def write_footer(self): """Write the file footer. If your file format defines a footer, you should implement this method in order to write the footer after all the records. The default implementation checks the private attribute ._footer_written to ensure the footer is only written once. """ assert self._header_written, "You must call write_header() first" assert ( self._record_written ), "You have not called write_record() or write_records() yet" assert not self._footer_written, "You have aleady called write_footer()" self._footer_written = True def write_record(self, record): """Write a single record to the output file. record - a SeqRecord object Once you have called write_header() you can call write_record() and/or write_records() as many times as needed. Then call write_footer() and close(). """ assert self._header_written, "You must call write_header() first" assert not self._footer_written, "You have already called write_footer()" self._record_written = True raise NotImplementedError("This object should be subclassed") ##################################################### # You SHOULD subclass this # ##################################################### def write_records(self, records): """Write multiple record to the output file. records - A list or iterator returning SeqRecord objects Once you have called write_header() you can call write_record() and/or write_records() as many times as needed. Then call write_footer() and close(). Returns the number of records written. """ # Default implementation: assert self._header_written, "You must call write_header() first" assert not self._footer_written, "You have already called write_footer()" count = 0 for record in records: self.write_record(record) count += 1 # Mark as true, even if there where no records self._record_written = True return count def write_file(self, records): """Use this to write an entire file containing the given records. records - A list or iterator returning SeqRecord objects This method can only be called once. Returns the number of records written. """ self.write_header() count = self.write_records(records) self.write_footer() return count