# # = sample/test_restriction_enzyme_long.rb - Benchmark tests for Bio::RestrictionEnzyme::Analysis.cut for long sequences # # Copyright:: Copyright (C) 2011 # Naohisa Goto # License:: The Ruby License # # Acknowledgements: The idea of the test is based on the issue report # https://github.com/bioruby/bioruby/issues/10 # posted by ray1729 (https://github.com/ray1729). # require 'test/unit' require 'benchmark' require 'bio' entry = Bio::TogoWS::REST.entry('genbank', 'BA000007.2') EcoliO157H7Seq = Bio::GenBank.new(entry).naseq.freeze module TestRestrictionEnzymeAnalysisCutLong # dummy benchmarch class class DummyBench def report(str); yield; end end module HelperMethods def _truncate_cut_ranges(cut_ranges, len) limit = len - 1 ret = cut_ranges.collect do |a| if a[0] > limit || a[2] > limit then nil else a.collect { |pos| pos > limit ? limit : pos } end end ret.compact! if last_a = ret[-1] then last_a[1] = limit last_a[3] = limit end ret end def _collect_cut_ranges(cuts) cuts.collect do |f| [ f.p_left, f.p_right, f.c_left, f.c_right ] end end def _test_by_size(len, bench = DummyBench.new) cuts = nil bench.report("#{self.class::TestLabel} #{len}") { cuts = _cut(self.class::SampleSequence[0, len]) } cut_ranges = _collect_cut_ranges(cuts) expected = _truncate_cut_ranges(self.class::SampleCutRanges, len) assert_equal(expected, cut_ranges) end def test_10k_to_100k $stderr.print "\n" Benchmark.bm(26) do |bench| 10_000.step(100_000, 10_000) do |len| _test_by_size(len, bench) end end end def test_100k_to_1M $stderr.print "\n" Benchmark.bm(26) do |bench| 100_000.step(1_000_000, 100_000) do |len| _test_by_size(len, bench) end end end def test_1M_to_5M_and_whole $stderr.print "\n" Benchmark.bm(26) do |bench| 1_000_000.step(5_000_000, 1_000_000) do |len| _test_by_size(len, bench) end _test_by_size(self.class::SampleSequence.length, bench) end end if defined? Bio::RestrictionEnzyme::SortedNumArray def disabled_test_whole cuts = _cut(self.class::SampleSequence) cut_ranges = _collect_cut_ranges(cuts) cut_ranges.each do |a| $stderr.print " [ ", a.join(", "), " ], \n" end assert_equal(self.class::SampleCutRanges, cut_ranges) end end #module HelperMethods class TestEcoliO157H7_BstEII < Test::Unit::TestCase include HelperMethods TestLabel = 'BstEII' SampleSequence = EcoliO157H7Seq SampleCutRanges = BstEII_WHOLE = [ [ 0, 79, 0, 84 ], [ 80, 4612, 85, 4617 ], [ 4613, 13483, 4618, 13488 ], [ 13484, 15984, 13489, 15989 ], [ 15985, 21462, 15990, 21467 ], [ 21463, 27326, 21468, 27331 ], [ 27327, 30943, 27332, 30948 ], [ 30944, 34888, 30949, 34893 ], [ 34889, 35077, 34894, 35082 ], [ 35078, 35310, 35083, 35315 ], [ 35311, 36254, 35316, 36259 ], [ 36255, 41885, 36260, 41890 ], [ 41886, 43070, 41891, 43075 ], [ 43071, 45689, 43076, 45694 ], [ 45690, 52325, 45695, 52330 ], [ 52326, 55703, 52331, 55708 ], [ 55704, 58828, 55709, 58833 ], [ 58829, 59178, 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5477861, 5478124, 5477866, 5478129 ], [ 5478125, 5478577, 5478130, 5478582 ], [ 5478578, 5479176, 5478583, 5479181 ], [ 5479177, 5483012, 5479182, 5483017 ], [ 5483013, 5483809, 5483018, 5483814 ], [ 5483810, 5495234, 5483815, 5495239 ], [ 5495235, 5498449, 5495240, 5498449 ] ] def _cut(seq) cuts = Bio::RestrictionEnzyme::Analysis.cut(seq, "BstEII", {:view_ranges => true}) end def test_BstEII_edge_cases (13481..13492).each do |len| _test_by_size(len) end end end # class TestEcoliO157H7_BstEII class TestEcoliO157H7_3enzymes < Test::Unit::TestCase include HelperMethods TestLabel = 'SacI+EcoRI+BstEII' SampleSequence = EcoliO157H7Seq SampleCutRanges = The3Enzymes_WHOLE = [ [ 0, 79, 0, 84 ], [ 80, 3858, 85, 3862 ], [ 3859, 4612, 3863, 4617 ], [ 4613, 5619, 4618, 5623 ], [ 5620, 7472, 5624, 7468 ], [ 7473, 12905, 7469, 12909 ], [ 12906, 13483, 12910, 13488 ], [ 13484, 14551, 13489, 14547 ], [ 14552, 15984, 14548, 15989 ], [ 15985, 20045, 15990, 20049 ], [ 20046, 21462, 20050, 21467 ], [ 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5478130, 5478582 ], [ 5478578, 5479176, 5478583, 5479181 ], [ 5479177, 5483012, 5479182, 5483017 ], [ 5483013, 5483809, 5483018, 5483814 ], [ 5483810, 5490967, 5483815, 5490963 ], [ 5490968, 5491739, 5490964, 5491743 ], [ 5491740, 5495234, 5491744, 5495239 ], [ 5495235, 5498449, 5495240, 5498449 ] ] def _cut(seq) cuts = Bio::RestrictionEnzyme::Analysis.cut(seq, "SacI", "EcoRI", "BstEII", {:view_ranges => true}) end end #TestEcoliO157H7_3enzymes end #module TestRestrictionEnzymeAnalysisCutLong