#include #include #include #include #include #include "bwt.h" #include "bwtsw2.h" #include "utils.h" #include "bwa.h" int bwa_bwtsw2(int argc, char *argv[]) { bsw2opt_t *opt; bwaidx_t *idx; int c; opt = bsw2_init_opt(); srand48(11); while ((c = getopt(argc, argv, "q:r:a:b:t:T:w:d:z:m:s:c:N:Hf:MI:SG:C")) >= 0) { switch (c) { case 'q': opt->q = atoi(optarg); break; case 'r': opt->r = atoi(optarg); break; case 'a': opt->a = atoi(optarg); break; case 'b': opt->b = atoi(optarg); break; case 'w': opt->bw = atoi(optarg); break; case 'T': opt->t = atoi(optarg); break; case 't': opt->n_threads = atoi(optarg); break; case 'z': opt->z = atoi(optarg); break; case 's': opt->is = atoi(optarg); break; case 'm': opt->mask_level = atof(optarg); break; case 'c': opt->coef = atof(optarg); break; case 'N': opt->t_seeds = atoi(optarg); break; case 'M': opt->multi_2nd = 1; break; case 'H': opt->hard_clip = 1; break; case 'f': xreopen(optarg, "w", stdout); break; case 'I': opt->max_ins = atoi(optarg); break; case 'S': opt->skip_sw = 1; break; case 'C': opt->cpy_cmt = 1; break; case 'G': opt->max_chain_gap = atoi(optarg); break; default: return 1; } } opt->qr = opt->q + opt->r; if (optind + 2 > argc) { fprintf(stderr, "\n"); fprintf(stderr, "Usage: bwa bwasw [options] [query2.fa]\n\n"); fprintf(stderr, "Options: -a INT score for a match [%d]\n", opt->a); fprintf(stderr, " -b INT mismatch penalty [%d]\n", opt->b); fprintf(stderr, " -q INT gap open penalty [%d]\n", opt->q); fprintf(stderr, " -r INT gap extension penalty [%d]\n", opt->r); fprintf(stderr, " -w INT band width [%d]\n", opt->bw); fprintf(stderr, " -m FLOAT mask level [%.2f]\n", opt->mask_level); fprintf(stderr, "\n"); fprintf(stderr, " -t INT number of threads [%d]\n", opt->n_threads); fprintf(stderr, " -f FILE file to output results to instead of stdout\n"); fprintf(stderr, " -H in SAM output, use hard clipping instead of soft clipping\n"); fprintf(stderr, " -C copy FASTA/Q comment to SAM output\n"); fprintf(stderr, " -M mark multi-part alignments as secondary\n"); fprintf(stderr, " -S skip Smith-Waterman read pairing\n"); fprintf(stderr, " -I INT ignore pairs with insert >=INT for inferring the size distr [%d]\n", opt->max_ins); fprintf(stderr, "\n"); fprintf(stderr, " -T INT score threshold divided by a [%d]\n", opt->t); fprintf(stderr, " -c FLOAT coefficient of length-threshold adjustment [%.1f]\n", opt->coef); fprintf(stderr, " -z INT Z-best [%d]\n", opt->z); fprintf(stderr, " -s INT maximum seeding interval size [%d]\n", opt->is); fprintf(stderr, " -N INT # seeds to trigger rev aln; 2*INT is also the chaining threshold [%d]\n", opt->t_seeds); fprintf(stderr, " -G INT maximum gap size during chaining [%d]\n", opt->max_chain_gap); fprintf(stderr, "\n"); fprintf(stderr, "Note: For long Illumina, 454 and Sanger reads, assembly contigs, fosmids and\n"); fprintf(stderr, " BACs, the default setting usually works well. For the current PacBio\n"); fprintf(stderr, " reads (end of 2010), '-b5 -q2 -r1 -z10' is recommended. One may also\n"); fprintf(stderr, " increase '-z' for better sensitivity.\n"); fprintf(stderr, "\n"); return 1; } // adjust opt for opt->a opt->t *= opt->a; opt->coef *= opt->a; if ((idx = bwa_idx_load(argv[optind], BWA_IDX_BWT|BWA_IDX_BNS)) == 0) return 1; bsw2_aln(opt, idx->bns, idx->bwt, argv[optind+1], optind+2 < argc? argv[optind+2] : 0); bwa_idx_destroy(idx); free(opt); return 0; }