Aligning contigs to reference... Aligning contigs to the reference Filtering alignments... Parsing coords... Loading reference... Loaded [meta_ref_1_Lactobacillus_plantarum_22_55k.fa] Loaded [meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa] Loaded [meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa] Total Regions: 3 Total Region Length: 179997 Analyzing contigs... CONTIG: NODE_1_length_55421_cov_26.1146_ID_4744408 (54221bp) Best alignment score: 36102.0 (LEN: 36102, IDY: 100.00), Total number of alignments: 2 Looking for the best set of alignments (out of 2 total alignments) The best set is below. Score: 53970.5, number of alignments: 2, unaligned bases: 0 This contig is misassembled. Excluding internal overlap of size 1 between Alignment 1 and 2: 41879 59998 | 18120 1 | 18120 18120 --> 41880 59998 | 18119 1 | 18119 18119 Real Alignment 1: 41880 59998 | 18119 1 | 18119 18119 | 100.0 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_1_length_55421_cov_26.1146_ID_4744408 Extensive misassembly (relocation, inconsistency = 1200) between these two alignments Real Alignment 2: 4578 40679 | 54221 18120 | 36102 36102 | 100.0 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_1_length_55421_cov_26.1146_ID_4744408 CONTIG: NODE_2_length_49658_cov_2.58581_ID_2497514 (49658bp) Best alignment score: 49648.1 (LEN: 49658, IDY: 99.98), Total number of alignments: 1 One align captures most of this contig: 5305 54962 | 1 49658 | 49658 49658 | 99.98 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_2_length_49658_cov_2.58581_ID_2497514 CONTIG: NODE_3_length_27260_cov_310.524_ID_9332986 (27260bp) Best alignment score: 27257.3 (LEN: 27260, IDY: 99.99), Total number of alignments: 1 One align captures most of this contig: 7597 34856 | 1 27260 | 27260 27260 | 99.99 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_3_length_27260_cov_310.524_ID_9332986 CONTIG: NODE_4_length_16099_cov_310.325_ID_9343456 (16099bp) Best alignment score: 16099.0 (LEN: 16099, IDY: 100.00), Total number of alignments: 1 One align captures most of this contig: 36665 52763 | 1 16099 | 16099 16099 | 100.0 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_4_length_16099_cov_310.325_ID_9343456 CONTIG: NODE_5_length_10428_cov_300.989_ID_9343584 (10428bp) Best alignment score: 10428.0 (LEN: 10428, IDY: 100.00), Total number of alignments: 1 One align captures most of this contig: 54572 64999 | 1 10428 | 10428 10428 | 100.0 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_5_length_10428_cov_300.989_ID_9343584 CONTIG: NODE_6_length_6988_cov_303.415_ID_9341226 (6988bp) Best alignment score: 6985.9 (LEN: 6988, IDY: 99.97), Total number of alignments: 4 Skipping these alignments as insignificant (option --ambiguity-score is set to "0.99"): Skipping alignment 329 3878 | 3427 6988 | 3550 3562 | 90.71 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_6_length_6988_cov_303.415_ID_9341226 Skipping alignment 3386 3627 | 6742 6986 | 242 245 | 92.28 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_6_length_6988_cov_303.415_ID_9341226 Skipping alignment 1 79 | 3349 3427 | 79 79 | 100.0 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_6_length_6988_cov_303.415_ID_9341226 One align captures most of this contig: 25 7012 | 1 6988 | 6988 6988 | 99.97 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_6_length_6988_cov_303.415_ID_9341226 CONTIG: NODE_7_length_3447_cov_44.4894_ID_2480336 (3447bp) Best alignment score: 3431.8 (LEN: 3447, IDY: 99.56), Total number of alignments: 1 One align captures most of this contig: 3 3449 | 3447 1 | 3447 3447 | 99.56 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_7_length_3447_cov_44.4894_ID_2480336 CONTIG: NODE_8_length_817_cov_651.256_ID_9343784 (817bp) Best alignment score: 813.0 (LEN: 817, IDY: 99.51), Total number of alignments: 2 This contig has 2 significant alignments. [An ambiguously mapped contig] Using all these alignments (option --ambiguity-usage is set to "all"): Alignment: 52709 53525 | 1 817 | 817 817 | 99.51 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_8_length_817_cov_651.256_ID_9343784 Alignment: 34802 35618 | 1 817 | 817 817 | 98.65 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_8_length_817_cov_651.256_ID_9343784 CONTIG: NODE_9_length_729_cov_671.122_ID_9343806 (729bp) Best alignment score: 729.0 (LEN: 729, IDY: 100.00), Total number of alignments: 2 Skipping these alignments as insignificant (option --ambiguity-score is set to "0.99"): Skipping alignment 53899 54626 | 728 1 | 728 728 | 98.9 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_9_length_729_cov_671.122_ID_9343806 One align captures most of this contig: 35991 36719 | 729 1 | 729 729 | 100.0 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_9_length_729_cov_671.122_ID_9343806 CONTIG: NODE_10_length_714_cov_2.71927_ID_9333852 (714bp) Best alignment score: 713.0 (LEN: 714, IDY: 99.86), Total number of alignments: 2 Skipping these alignments as insignificant (option --ambiguity-score is set to "0.99"): Skipping alignment 1 123 | 464 342 | 123 123 | 99.19 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_10_length_714_cov_2.71927_ID_9333852 One align captures most of this contig: 78 791 | 714 1 | 714 714 | 99.86 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_10_length_714_cov_2.71927_ID_9333852 CONTIG: NODE_11_length_694_cov_336.263_ID_9343406 (694bp) Best alignment score: 693.0 (LEN: 694, IDY: 99.86), Total number of alignments: 3 Skipping these alignments as insignificant (option --ambiguity-score is set to "0.99"): Skipping alignment 3824 4517 | 1 694 | 694 694 | 93.85 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_11_length_694_cov_336.263_ID_9343406 Skipping alignment 3575 4268 | 1 694 | 694 694 | 93.24 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_11_length_694_cov_336.263_ID_9343406 One align captures most of this contig: 6958 7651 | 1 694 | 694 694 | 99.86 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_11_length_694_cov_336.263_ID_9343406 CONTIG: NODE_29_length_219_cov_25.5122_ID_8985538 (763bp) Best alignment score: 219.0 (LEN: 219, IDY: 100.00), Total number of alignments: 9 Looking for the best set of alignments (out of 9 total alignments) Skipping redundant alignments after choosing the best set of alignments Skipping redundant alignment 7777 7983 | 1 207 | 207 207 | 90.82 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Skipping redundant alignment 4643 4861 | 1 219 | 219 219 | 90.13 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Skipping redundant alignment 3395 3573 | 428 605 | 179 178 | 91.62 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Skipping redundant alignment 6775 6961 | 428 610 | 187 183 | 91.44 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Skipping redundant alignment 5124 5251 | 636 763 | 128 128 | 96.88 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_29_length_219_cov_25.5122_ID_8985538 The best set is below. Score: 597.1, number of alignments: 4, unaligned bases: 0 This contig is misassembled. Real Alignment 1: 4394 4612 | 1 219 | 219 219 | 100.0 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Extensive misassembly (interspecies translocation) between these two alignments Real Alignment 2: 21946 22153 | 427 220 | 208 208 | 98.08 | meta_ref_3_Lactobacillus_reuteri_F275_Kitasato_55_65k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Extensive misassembly (interspecies translocation) between these two alignments Excluding internal overlap of size 2 between Alignment 3 and 4: 1717 1871 | 609 763 | 155 155 --> 1719 1871 | 611 763 | 153 153 Real Alignment 3: 3645 3827 | 428 610 | 183 183 | 100.0 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Extensive misassembly (interspecies translocation) between these two alignments Real Alignment 4: 1719 1871 | 611 763 | 153 153 | 96.77 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_29_length_219_cov_25.5122_ID_8985538 CONTIG: NODE_35_specially_unaligned (885bp) This contig is unaligned. (885 bp) Unaligned bases: 885 (number of Ns: 0) Analyzing coverage... Writing SNPs into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/combined_reference/contigs_reports/minimap_output/meta_contigs_2.used_snps Analysis is finished! Founded SNPs were written into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/combined_reference/contigs_reports/minimap_output/meta_contigs_2.used_snps Results: Local Misassemblies: 0 Misassemblies: 4 Relocations: 1 Translocations: 0 Interspecies translocations: 3 Inversions: 0 Potentially Misassembled Contigs (i/s translocations): 0 Possible Misassemblies: 0 Scaffold gap extensive misassemblies: 0 Scaffold gap local misassemblies: 0 Misassembled Contigs: 2 Misassembled Contig Bases: 54984 Misassemblies Inter-Contig Overlap ("Extra" Aligned Bases): 0 Unaligned Contigs: 1 + 0 part Half Unaligned Contigs with Misassemblies: 0 Unaligned Bases in Fully and Partially Unaligned Contigs: 885 Ambiguously Mapped Contigs: 1 Total Bases in Ambiguously Mapped Contigs: 817 "Extra" Aligned Bases in Ambiguously Mapped Contigs: 817 Note that --allow-ambiguity option was set to "all" and each of these contigs was used several times (there are "extra" aligned bases). Covered Bases in Reference: 171964 Raw Aligned Bases in Assembly: 171818 Total Aligned Bases in Assembly (with "Extras"): 172635 SNPs: 57 Insertions: 0 Deletions: 0