/* test/merge/test_bam_translate.c -- header merging test harness. Copyright (C) 2013, 2014 Genome Research Ltd. Author: Martin O. Pollard Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include "../../bam_sort.c" #include "../test.h" #include #include #include #include #include void dump_read(bam1_t* b) { printf("->core.tid:(%d)\n", b->core.tid); printf("->core.pos:(%d)\n", b->core.pos); printf("->core.bin:(%d)\n", b->core.bin); printf("->core.qual:(%d)\n", b->core.qual); printf("->core.l_qname:(%d)\n", b->core.l_qname); printf("->core.flag:(%d)\n", b->core.flag); printf("->core.n_cigar:(%d)\n", b->core.n_cigar); printf("->core.l_qseq:(%d)\n", b->core.l_qseq); printf("->core.mtid:(%d)\n", b->core.mtid); printf("->core.mpos:(%d)\n", b->core.mpos); printf("->core.isize:(%d)\n", b->core.isize); if (b->data) { printf("->data:"); int i; for (i = 0; i < b->l_data; ++i) { printf("%x ", b->data[i]); } printf("\n"); } if (b->core.l_qname) { printf("qname: %s\n",bam_get_qname(b)); } if (b->core.l_qseq) { printf("qseq:"); int i; for (i = 0; i < b->core.l_qseq; ++i) { printf("%c",seq_nt16_str[seq_nt16_table[bam_seqi(bam_get_seq(b),i)]]); } printf("\n"); printf("qual:"); for (i = 0; i < b->core.l_qseq; ++i) { printf("%c",bam_get_qual(b)[i]); } printf("\n"); } if (bam_get_l_aux(b)) { int i = 0; uint8_t* aux = bam_get_aux(b); while (i < bam_get_l_aux(b)) { printf("%.2s:%c:",aux+i,*(aux+i+2)); i += 2; switch (*(aux+i)) { case 'Z': while (*(aux+1+i) != '\0') { putc(*(aux+1+i), stdout); ++i; } break; } putc('\n',stdout); ++i;++i; } } printf("\n"); } void trans_tbl_test_init(trans_tbl_t* tbl, int32_t n_targets) { tbl->tid_trans = (int*)calloc(n_targets, sizeof(int32_t)); tbl->rg_trans = kh_init(c2c); tbl->pg_trans = kh_init(c2c); } void setup_test_1(bam1_t** b_in, trans_tbl_t* tbl) { bam1_t* b; b = bam_init1(); trans_tbl_test_init(tbl, 4); tbl->tid_trans[0] = 5; tbl->tid_trans[1] = 6; tbl->tid_trans[2] = 7; tbl->tid_trans[3] = 8; b->core.tid = 0; b->core.pos = 1334; b->core.bin = 0; b->core.qual = 10; b->core.l_qname = 10; b->core.flag = 0; b->core.n_cigar = 1; b->core.l_qseq = 10; b->core.mtid = -1; b->core.mpos = 0; b->core.isize = -1; size_t data_len = 10 + 4 + 5 + 10 + 0; b->data = (uint8_t*)malloc(data_len); memcpy(b->data, "123456789\0" // q_name "\x00\x00\x00\xA0" // cigar "\x00\x00\x00\x00\x00" // qseq "\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF" // qual "" // aux , data_len ); b->m_data = b->l_data = data_len; *b_in = b; } void setup_test_2(bam1_t** b_in, trans_tbl_t* tbl) { bam1_t* b; b = bam_init1(); trans_tbl_test_init(tbl, 4); tbl->tid_trans[0] = 5; tbl->tid_trans[1] = 6; tbl->tid_trans[2] = 7; tbl->tid_trans[3] = 8; int in_there = 0; khiter_t iter = kh_put(c2c, tbl->rg_trans, strdup("hello"), &in_there); kh_value(tbl->rg_trans, iter) = strdup("goodbye"); b->core.tid = 0; b->core.pos = 1334; b->core.bin = 0; b->core.qual = 10; b->core.l_qname = 10; b->core.flag = 0; b->core.n_cigar = 1; b->core.l_qseq = 10; b->core.mtid = -1; b->core.mpos = 0; b->core.isize = -1; size_t data_len = 10 + 4 + 5 + 10 + 9; b->data = (uint8_t*)malloc(data_len); memcpy(b->data, "123456789\0" // q_name "\x00\x00\x00\xA0" // cigar "\x00\x00\x00\x00\x00" // qseq "\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF" // qual "RGZhello\0" // aux , data_len ); b->m_data = b->l_data = data_len; *b_in = b; } void setup_test_3(bam1_t** b_in, trans_tbl_t* tbl) { bam1_t* b; b = bam_init1(); trans_tbl_test_init(tbl, 4); tbl->tid_trans[0] = 5; tbl->tid_trans[1] = 6; tbl->tid_trans[2] = 7; tbl->tid_trans[3] = 8; int in_there = 0; khiter_t iter = kh_put(c2c, tbl->pg_trans, strdup("hello"), &in_there); kh_value(tbl->pg_trans,iter) = strdup("goodbye"); b->core.tid = 0; b->core.pos = 1334; b->core.bin = 0; b->core.qual = 10; b->core.l_qname = 10; b->core.flag = 0; b->core.n_cigar = 1; b->core.l_qseq = 10; b->core.mtid = -1; b->core.mpos = 0; b->core.isize = -1; size_t data_len = 10 + 4 + 5 + 10 + 9; b->data = (uint8_t*)malloc(data_len); memcpy(b->data, "123456789\0" // q_name "\x00\x00\x00\xA0" // cigar "\x00\x00\x00\x00\x00" // qseq "\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF" // qual "PGZhello\0" // aux , data_len ); b->m_data = b->l_data = data_len; *b_in = b; } void setup_test_4(bam1_t** b_in, trans_tbl_t* tbl) { bam1_t* b; b = bam_init1(); trans_tbl_test_init(tbl, 4); tbl->tid_trans[0] = 5; tbl->tid_trans[1] = 6; tbl->tid_trans[2] = 7; tbl->tid_trans[3] = 8; b->core.tid = 0; b->core.pos = 1334; b->core.bin = 0; b->core.qual = 10; b->core.l_qname = 10; b->core.flag = 0; b->core.n_cigar = 1; b->core.l_qseq = 10; b->core.mtid = -1; b->core.mpos = 0; b->core.isize = -1; size_t data_len = 10 + 4 + 5 + 10 + 12; b->data = (uint8_t*)malloc(data_len); memcpy(b->data, "123456789\0" // q_name "\x00\x00\x00\xA0" // cigar "\x00\x00\x00\x00\x00" // qseq "\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF" // qual "RGZrg4hello\0" // aux , data_len ); b->m_data = b->l_data = data_len; *b_in = b; } void setup_test_5(bam1_t** b_in, trans_tbl_t* tbl) { bam1_t* b; b = bam_init1(); trans_tbl_test_init(tbl, 4); tbl->tid_trans[0] = 5; tbl->tid_trans[1] = 6; tbl->tid_trans[2] = 7; tbl->tid_trans[3] = 8; b->core.tid = 0; b->core.pos = 1334; b->core.bin = 0; b->core.qual = 10; b->core.l_qname = 10; b->core.flag = 0; b->core.n_cigar = 1; b->core.l_qseq = 10; b->core.mtid = -1; b->core.mpos = 0; b->core.isize = -1; size_t data_len = 10 + 4 + 5 + 10 + 12; b->data = (uint8_t*)malloc(data_len); memcpy(b->data, "123456789\0" // q_name "\x00\x00\x00\xA0" // cigar "\x00\x00\x00\x00\x00" // qseq "\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF" // qual "PGZpg5hello\0" // aux , data_len ); b->m_data = b->l_data = data_len; *b_in = b; } void setup_test_6(bam1_t** b_in, trans_tbl_t* tbl) { bam1_t* b; b = bam_init1(); trans_tbl_test_init(tbl, 4); tbl->tid_trans[0] = 5; tbl->tid_trans[1] = 6; tbl->tid_trans[2] = 7; tbl->tid_trans[3] = 8; int in_there = 0; khiter_t iter_rg = kh_put(c2c, tbl->rg_trans, strdup("hello"), &in_there); kh_value(tbl->rg_trans, iter_rg) = strdup("goodbye"); khiter_t iter_pg = kh_put(c2c, tbl->pg_trans, strdup("quail"), &in_there); kh_value(tbl->pg_trans, iter_pg) = strdup("bird"); b->core.tid = 0; b->core.pos = 1334; b->core.bin = 0; b->core.qual = 10; b->core.l_qname = 10; b->core.flag = 0; b->core.n_cigar = 1; b->core.l_qseq = 10; b->core.mtid = -1; b->core.mpos = 0; b->core.isize = -1; size_t data_len = 10 + 4 + 5 + 10 + 18; b->data = (uint8_t*)malloc(data_len); memcpy(b->data, "123456789\0" // q_name "\x00\x00\x00\xA0" // cigar "\x00\x00\x00\x00\x00" // qseq "\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF\xFF" // qual "RGZhello\0PGZquail\0" // aux , data_len ); b->m_data = b->l_data = data_len; *b_in = b; } int main(int argc, char**argv) { // test state const int NUM_TESTS = 6; int verbose = 0; int success = 0; int failure = 0; int getopt_char; while ((getopt_char = getopt(argc, argv, "v")) != -1) { switch (getopt_char) { case 'v': ++verbose; break; default: break; } } bam1_t* b; // Setup stderr redirect kstring_t res = { 0, 0, NULL }; FILE* orig_stderr = fdopen(dup(STDERR_FILENO), "a"); // Save stderr char* tempfname = (optind < argc)? argv[optind] : "test_bam_translate.tmp"; FILE* check = NULL; // setup if (verbose) printf("BEGIN test 1\n"); // TID test trans_tbl_t tbl1; setup_test_1(&b,&tbl1); if (verbose > 1) { printf("b\n"); dump_read(b); } if (verbose) printf("RUN test 1\n"); // test xfreopen(tempfname, "w", stderr); // Redirect stderr to pipe bam_translate(b, &tbl1); fclose(stderr); if (verbose) printf("END RUN test 1\n"); if (verbose > 1) { printf("b\n"); dump_read(b); } // check result check = fopen(tempfname, "r"); res.l = 0; if (kgetline(&res, (kgets_func *)fgets, check) < 0 && (feof(check) || res.l == 0) ) { ++success; } else { ++failure; if (verbose) printf("FAIL test 1\n"); } fclose(check); // teardown bam_destroy1(b); trans_tbl_destroy(&tbl1); if (verbose) printf("END test 1\n"); // setup if (verbose) printf("BEGIN test 2\n"); // RG exists and translate test trans_tbl_t tbl2; setup_test_2(&b,&tbl2); if (verbose > 1) { printf("b\n"); dump_read(b); } if (verbose) printf("RUN test 2\n"); // test xfreopen(tempfname, "w", stderr); // Redirect stderr to pipe bam_translate(b, &tbl2); fclose(stderr); if (verbose) printf("END RUN test 2\n"); if (verbose > 1) { printf("b\n"); dump_read(b); } // check result check = fopen(tempfname, "r"); res.l = 0; if (kgetline(&res, (kgets_func *)fgets, check) < 0 && (feof(check) || res.l == 0) ) { ++success; } else { ++failure; if (verbose) printf("FAIL test 2\n"); } fclose(check); // teardown bam_destroy1(b); trans_tbl_destroy(&tbl2); if (verbose) printf("END test 2\n"); if (verbose) printf("BEGIN test 3\n"); // PG exists and translate test // setup trans_tbl_t tbl3; setup_test_3(&b,&tbl3); if (verbose > 1) { printf("b\n"); dump_read(b); } if (verbose) printf("RUN test 3\n"); // test xfreopen(tempfname, "w", stderr); // Redirect stderr to pipe bam_translate(b, &tbl3); fclose(stderr); if (verbose) printf("END RUN test 3\n"); if (verbose > 1) { printf("b\n"); dump_read(b); } // check result check = fopen(tempfname, "r"); res.l = 0; if (kgetline(&res, (kgets_func *)fgets, check) < 0 && (feof(check) || res.l == 0)) { ++success; } else { ++failure; if (verbose) printf("FAIL test 3\n"); } fclose(check); // teardown bam_destroy1(b); trans_tbl_destroy(&tbl3); if (verbose) printf("END test 3\n"); if (verbose) printf("BEGIN test 4\n"); // RG test non-existent // setup trans_tbl_t tbl4; setup_test_4(&b,&tbl4); if (verbose > 1) { printf("b\n"); dump_read(b); } if (verbose) printf("RUN test 4\n"); // test xfreopen(tempfname, "w", stderr); // Redirect stderr to pipe bam_translate(b, &tbl4); fclose(stderr); if (verbose) printf("END RUN test 4\n"); if (verbose > 1) { printf("b\n"); dump_read(b); } // check result check = fopen(tempfname, "r"); res.l = 0; if (kgetline(&res, (kgets_func *)fgets, check) >= 0 && strcmp("[bam_translate] RG tag \"rg4hello\" on read \"123456789\" encountered with no corresponding entry in header, tag lost. Unknown tags are only reported once per input file for each tag ID.",res.s) == 0) { ++success; } else { ++failure; if (verbose) printf("FAIL test 4\n"); } fclose(check); // teardown bam_destroy1(b); trans_tbl_destroy(&tbl4); if (verbose) printf("END test 4\n"); if (verbose) printf("BEGIN test 5\n"); // PG test non-existent // setup trans_tbl_t tbl5; setup_test_5(&b,&tbl5); if (verbose > 1) { printf("b\n"); dump_read(b); printf("RUN test 5\n"); } // test xfreopen(tempfname, "w", stderr); // Redirect stderr to pipe bam_translate(b, &tbl5); fclose(stderr); if (verbose) printf("END RUN test 5\n"); if (verbose > 1) { printf("b\n"); dump_read(b); } // check result check = fopen(tempfname, "r"); res.l = 0; if (kgetline(&res, (kgets_func *)fgets, check) >= 0 && strcmp("[bam_translate] PG tag \"pg5hello\" on read \"123456789\" encountered with no corresponding entry in header, tag lost. Unknown tags are only reported once per input file for each tag ID.",res.s) == 0) { ++success; } else { ++failure; if (verbose) printf("FAIL test 5\n"); } fclose(check); // teardown bam_destroy1(b); trans_tbl_destroy(&tbl5); if (verbose) printf("END test 5\n"); if (verbose) printf("BEGIN test 6\n"); // RG and PG exists and translate test // setup trans_tbl_t tbl6; setup_test_6(&b,&tbl6); if (verbose > 1) { printf("b\n"); dump_read(b); } if (verbose) printf("RUN test 6\n"); // test xfreopen(tempfname, "w", stderr); // Redirect stderr to pipe bam_translate(b, &tbl6); fclose(stderr); if (verbose) printf("END RUN test 6\n"); if (verbose > 1) { printf("b\n"); dump_read(b); } // check result check = fopen(tempfname, "r"); res.l = 0; if (kgetline(&res, (kgets_func *)fgets, check) < 0 && (feof(check) || res.l == 0) ) { ++success; } else { ++failure; if (verbose) printf("FAIL test 6\n"); } fclose(check); // teardown bam_destroy1(b); trans_tbl_destroy(&tbl6); if (verbose) printf("END test 6\n"); // Cleanup free(res.s); remove(tempfname); if (failure > 0) fprintf(orig_stderr, "%d failures %d successes\n", failure, success); fclose(orig_stderr); return (success == NUM_TESTS)? EXIT_SUCCESS : EXIT_FAILURE; }