| Sequence (5'->3') | Template strand | Length | Start | Stop | Tm | GC% | Self complementarity | Self 3' complementarity | |
|---|---|---|---|---|---|---|---|---|---|
| Forward primer | GTGGCTTCTCCGTTTCTTGC | Plus | 20 | 17537355 | 17537374 | 59.76 | 55.00 | 2.00 | 2.00 |
| Reverse primer | TGGTGGTGGTTAGTTGAACCG | Minus | 21 | 17542648 | 17542628 | 60.48 | 52.38 | 5.00 | 2.00 |
| Product length | |||||||||