LOCUS PBI101TD 5349 bp DNA linear SYN 06-MAY-2008 DEFINITION GUS gene fusion vector pBI101 T-DNA region. ACCESSION U12639 VERSION U12639.1 GI:2088506 KEYWORDS pBI101; T-DNA; GUS gene fusion vector; neomycin phosphotransferase; beta-glucuronidase. SOURCE Cloning vector pBI101 ORGANISM Cloning vector pBI101 other sequences; artificial sequences; vectors. REFERENCE 1 (sites) AUTHORS Jefferson,R.A., Burgess,S.M. and Hirsh,D. TITLE beta-Glucuronidase from Escherichia coli as a gene-fusion marker JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83 (22), 8447-8451 (1986) PUBMED 3534890 REFERENCE 2 (bases 2497 to 2556) AUTHORS Jefferson,R. TITLE Assaying chimeric genes in plants: the GUS gene fusion system JOURNAL Plant Mol. Biol. Rep. 5, 387-405 (1987) REFERENCE 3 (bases 1 to 5349) AUTHORS Jefferson,R.A., Kavanagh,T.A. and Bevan,M.W. TITLE GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants JOURNAL EMBO J. 6 (13), 3901-3907 (1987) PUBMED 3327686 REFERENCE 4 (bases 1 to 5349) AUTHORS Wei,W. and Lindsey,K. TITLE T-DNA sequence of the GUS gene fusion vector pBI101 JOURNAL Unpublished REFERENCE 5 (bases 1 to 5349) AUTHORS Wei,W. TITLE Direct Submission JOURNAL Submitted (22-JUL-1994) Wenbin Wei, Botany, University of Leicester, University Road, Leicester, LE1 7RH, UK COMMENT On May 14, 1997 this sequence version replaced gi:529328. FEATURES Location/Qualifiers source 1..5349 /organism="Cloning vector pBI101" /mol_type="other DNA" /db_xref="taxon:36566" /note="sequence from the right border to the left border" misc_feature 1..2497 /note="pBin19 sequence, containing a neomycin phosphotransferase gene driven by nos promoter" repeat_region 1..25 /note="the right border repeat" CDS 385..1179 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAC53707.1" /db_xref="GI:2102680" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 2497..2537 /note="polylinker" CDS 2551..4359 /note="modified beta-glucuronidase gene coding region from pRAJ260" /codon_start=1 /transl_table=11 /product="beta-glucuronidase" /protein_id="AAC53706.1" /db_xref="GI:529330" /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI KELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDA HTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ" 3'UTR 4430..4682 /note="3'UTR of the nopaline synthase gene" misc_feature 4686..5349 /note="pBin19 sequence (see also GenBank Accession Number U09365)" repeat_region 5325..5349 /note="the left border repeat" ORIGIN 1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac 61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg 121 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc 181 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa 241 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt 301 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc 361 tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg 601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 901 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc 961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 1021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 1081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 1141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 1201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 1261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 1321 gggatctcat gctggagttc ttcgcccacg ggatctctgc ggaacaggcg gtcgaaggtg 1381 ccgatatcat tacgacagca acggccgaca agcacaacgc cacgatcctg agcgacaata 1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga 1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga 1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg 1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca 1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca 1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg 1801 tgtcatctat gttactagat cgggcctcct gtcaatgctg gcggcggctc tggtggtggt 1861 tctggtggcg gctctgaggg tggtggctct gagggtggcg gttctgaggg tggcggctct 1921 gagggaggcg gttccggtgg tggctctggt tccggtgatt ttgattatga aaagatggca 1981 aacgctaata agggggctat gaccgaaaat gccgatgaaa acgcgctaca gtctgacgct 2041 aaaggcaaac ttgattctgt cgctactgat tacggtgctg ctatcgatgg tttcattggt 2101 gacgtttccg gccttgctaa tggtaatggt gctactggtg attttgctgg ctctaattcc 2161 caaatggctc aagtcggtga cggtgataat tcacctttaa tgaataattt ccgtcaatat 2221 ttaccttccc tccctcaatc ggttgaatgt cgcccttttg tctttggccc aatacgcaaa 2281 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 2341 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 2401 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 2461 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac 2521 tctagaggat ccccgggtgg tcagtccctt atgttacgtc ctgtagaaac cccaacccgt 2581 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt 2641 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt 2701 tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag 2761 cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg 2821 gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc 2881 tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc 2941 accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc 3001 gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccggaatc 3061 catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg 3121 acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt 3181 gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact 3241 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat 3301 gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc 3361 atccggtcag tggcagtgaa gggccaacag ttcctgatta accacaaacc gttctacttt 3421 actggctttg gtcgtcatga agatgcggac ttacgtggca aaggattcga taacgtgctg 3481 atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat 3541 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat 3601 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag 3661 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag 3721 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt 3781 gccaacgaac cggatacccg tccgcaagtg cacgggaata tttcgccact ggcggaagca 3841 acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct 3901 cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg 3961 tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga acttctggcc 4021 tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga tacgttagcc 4081 gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat 4141 atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc 4201 gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa agggatcttc 4261 actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gactggcatg 4321 aacttcggtg aaaaaccgca gcagggaggc aaacaatgaa tcaacaactc tcctggcgca 4381 ccatcgtcgg ctacagcctc gggaattgct accgagctcg aatttccccg atcgttcaaa 4441 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat 4501 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt 4561 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa 4621 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga 4681 tcgggaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 4741 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 4801 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc 4861 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 4921 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt 4981 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 5041 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 5101 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt 5161 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg 5221 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt 5281 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat 5341 atcctgcca // LOCUS pBSGUS 4809 bp DNA circular SYN 17-JAN-2000 DEFINITION pBSGUS - GUS gene on BamHI/SacI fragment from pBI101 cloned into pBluescriptKSm13+. ACCESSION pBSGUS KEYWORDS . SOURCE synthetic construct. ORGANISM synthetic construct artificial sequence. REFERENCE 1 (bases 1 to 4809) AUTHORS Fristensky,B. TITLE Untitled JOURNAL Unpublished REFERENCE 2 (bases 1 to 4809) AUTHORS Fristensky,B. TITLE Direct Submission JOURNAL Submitted (17-JAN-2000) Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada FEATURES Location/Qualifiers source 1..4809 /organism="synthetic construct" source 1..1831 /organism="synthetic construct" /plasmid="pBI101" /note="U12639:2528..4418" CDS 24..1832 /codon_start=1 /transl_table=11 /product="beta glucuronidase" /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI KELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDA HTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ" source 1833..4809 /organism="synthetic construct" /plasmid="pBluescriptKSm13+" /note="U52331:complement(653..694)" BASE COUNT 1200 a 1201 c 1249 g 1159 t ORIGIN 1 gatccccggg tggtcagtcc cttatgttac gtcctgtaga aaccccaacc cgtgaaatca 61 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc 121 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg 181 atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag 241 tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc 301 attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc 361 catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt 421 gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa 481 acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca 541 gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg 601 tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca 661 gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga 721 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt 781 gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt 841 cagtggcagt gaagggccaa cagttcctga ttaaccacaa accgttctac tttactggct 901 ttggtcgtca tgaagatgcg gacttacgtg gcaaaggatt cgataacgtg ctgatggtgc 961 acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt 1021 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg 1081 ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag 1141 aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta 1201 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg 1261 aaccggatac ccgtccgcaa gtgcacggga atatttcgcc actggcggaa gcaacgcgta 1321 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg 1381 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc 1441 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg 1501 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc 1561 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc 1621 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt 1681 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg 1741 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg 1801 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt 1861 cggctacagc ctcgggaatt gctaccgagc tccaattcgc cctatagtga gtcgtattac 1921 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 1981 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 2041 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc 2101 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 2161 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 2221 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 2281 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 2341 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 2401 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 2461 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 2521 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 2581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 2641 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 2701 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 2761 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 2821 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 2881 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 2941 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 3001 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 3061 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 3121 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 3181 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 3241 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 3301 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 3361 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 3421 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 3481 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 3541 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 3601 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 3661 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 3721 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 3781 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 3841 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 3901 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 3961 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 4021 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 4081 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 4141 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 4201 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 4261 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 4321 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 4381 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 4441 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 4501 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 4561 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 4621 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 4681 acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc 4741 tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc 4801 agcccgggg // LOCUS PEACAB15 822 bp mRNA linear PLN 27-APR-1993 DEFINITION Pea major chlorophyll a/b-binding thylakoid protein (polypeptide 15) mRNA, clone pAB96. ACCESSION J01253 VERSION J01253.1 GI:169050 KEYWORDS chlorophyll-binding protein; major chlorophyll binding protein; polypeptide 15. SOURCE Pisum sativum (pea) ORGANISM Pisum sativum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Fabeae; Pisum. REFERENCE 1 (bases 1 to 822) AUTHORS Coruzzi,G., Broglie,R., Cashmore,A. and Chua,N.H. TITLE Nucleotide sequences of two pea cDNA clones encoding the small subunit of ribulose 1,5-bisphosphate carboxylase and the major chlorophyll a/b-binding thylakoid polypeptide JOURNAL J. Biol. Chem. 258 (3), 1399-1402 (1983) PUBMED 6296093 COMMENT Original source text: Pea, cDNA to mRNA, clone pAB96. The mRNA encoding polypeptide 15 of the light-harvesting chlorophyll a/b-protein complex is translated on free cytoplasmic polysomes as a larger precursor, which is imported post-translationally into chloroplasts by an ATP-dependent process. The precursor chain extension (transit sequence) is thought to function in the post-translational translocation across the chloroplast envelope. [1] also reported the cDNA clone pSS15 (separate entry) which encodes the small subunit of ribulose 1,5-bisphosphate carboxylase. FEATURES Location/Qualifiers source 1..822 /organism="Pisum sativum" /mol_type="mRNA" /db_xref="taxon:3888" mRNA <1..822 /product="polypeptide 15 mRNA" CDS <1..688 /note="polypeptide 15 precursor" /codon_start=2 /protein_id="AAA33650.1" /db_xref="GI:169051" /translation="TTKKVASSSSPWHGPDGVKYLGPFSGESPSYLTGEFPGDYGWDT AGLSADPETFAKNRELEVIHSRWAMLGALGCVFPELLSRNGVKFGEAVWFKAGSQIFS EGGLDYLGNPSLVHAQSILAIWATQVILMGAVEGYRIAGGPLGEVVDPLYPGGSFDPL GLAEVPEAFAELKVKELKNGRLAMFSMFGFFVPAIVTGKGPLENLADHLADPVNNNAW SYATNFVPGK" mat_peptide <1..685 /product="polypeptide 15" ORIGIN 1 caccaccaag aaagtagcct cctctagtag cccatggcac ggcccagacg gtgttaagta 61 cctaggccca ttctccggtg agtctccatc atacttgacc ggagagttcc caggtgacta 121 cggttgggac actgctggac tttctgccga tccagagaca tttgccaaga accgtgagct 181 tgaagttatc cactccagat gggctatgtt gggagccttg ggatgtgtct tcccagagct 241 tttgtctcgc aacggtgtta aattcggtga agctgtatgg ttcaaggcag gatctcaaat 301 ctttagcgag ggtggacttg actacttggg caacccaagc ttggtccatg ctcaaagcat 361 ccttgccatc tgggccactc aggttatctt gatgggagct gttgaaggtt accgtattgc 421 cggtggccct cttggtgagg ttgttgaccc actttatcct ggtggtagct ttgatccatt 481 aggcttagct gaagtaccag aagcttttgc agaattaaag gtaaaggaac tgaagaacgg 541 tagattagcc atgttctcta tgtttggttt ctttgttcca gctattgtaa ctggaaaggg 601 tcctttggag aaccttgccg atcatcttgc cgacccagtg aacaacaacg catggtcata 661 tgctaccaac tttgttcccg gaaagtgaac actattatgt ttatgttatt ggtgaagtat 721 gttattgtta ttgtaatgtt cttccaattt aatgtgaatt gttatggtta tcgtgtgtgt 781 atgcgtaggt taactaatat gaactgattc gttttttttt tc // LOCUS X52331 2958 bp DNA circular SYN 29-JAN-2002 DEFINITION pBluescript KS(+) vector DNA, phagemid excised from lambda ZAP. ACCESSION X52331 VERSION X52331.1 GI:58065 KEYWORDS artificial sequence; cloning vector; expression vector; vector. SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. REFERENCE 1 AUTHORS Short,J.M., Fernandez,J.M., Sorge,J.A. and Huse,W.D. TITLE Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties JOURNAL Nucleic Acids Res. 16 (15), 7583-7600 (1988) PUBMED 2970625 REFERENCE 2 AUTHORS Alting-Mees,M.A. and Short,J.M. TITLE pBluescript II: gene mapping vectors JOURNAL Nucleic Acids Res. 17 (22), 9494 (1989) PUBMED 2555794 REFERENCE 3 AUTHORS Lampe,D.J., Grant,T.E. and Robertson,H.M. TITLE Factors affecting transposition of the Himar1 mariner transposon in vitro JOURNAL Genetics 149 (1), 179-187 (1998) PUBMED 9584095 REFERENCE 4 (bases 1 to 2958) AUTHORS Thomas,E.A. TITLE Direct Submission JOURNAL Submitted (20-FEB-1990) Thomas E.A., Stratagene Clonin Systems, 11099 North Torney Pines Rd., La Jolla, CA 92037, USA FEATURES Location/Qualifiers source 1..2958 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" misc_feature 1..2958 /note="phagemid pBluescript KS(+)" ORIGIN 1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa 721 gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt 781 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 1921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 1981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 2041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 2101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 2161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 2221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 2281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 2341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 2401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 2461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 2521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 2581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 2641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 2701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 2761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 2821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 2881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 2941 catttccccg aaaagtgc //