LOCUS       pBS_SK-GUS              5978 bp     circular            27-FEB-2024
UNIMARK     pBS_SK-GUS annotations
            pBS_SK-GUS
FEATURES             Location/Qualifiers
     misc_feature    1..3028
                     /source_doc="pBI121.gen"
                     /ugene_name="AF485783\ Fragment\ 2"
     misc_feature    1..5978
                     /comment="Molecule is created with Unipro UGENE v49.1"
                     /ugene_name="source"
     regulatory      20..854
                     /regulatory_class="promoter"
                     /note="CaMV 35S"
     gene            891..2702
                     /gene="gusA"
     CDS             891..2702
                     /gene="gusA"
                     /note="GUS"
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /protein_id="AAL92040.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     regulatory      2773..3025
                     /regulatory_class="terminator"
                     /note="NOS"
     misc_feature    3033..5974
                     /source_doc="pBS_SK-.gen"
                     /ugene_name="X52324\ Fragment\ 1"
ORIGIN
        1 TGCATGCCTG CAGGTCCCCA GATTAGCCTT TTCAATTTCA GAAAGAATGC TAACCCACAG
       61 ATGGTTAGAG AGGCTTACGC AGCAGGTCTC ATCAAGACGA TCTACCCGAG CAATAATCTC
      121 CAGGAAATCA AATACCTTCC CAAGAAGGTT AAAGATGCAG TCAAAAGATT CAGGACTAAC
      181 TGCATCAAGA ACACAGAGAA AGATATATTT CTCAAGATCA GAAGTACTAT TCCAGTATGG
      241 ACGATTCAAG GCTTGCTTCA CAAACCAAGG CAAGTAATAG AGATTGGAGT CTCTAAAAAG
      301 GTAGTTCCCA CTGAATCAAA GGCCATGGAG TCAAAGATTC AAATAGAGGA CCTAACAGAA
      361 CTCGCCGTAA AGACTGGCGA ACAGTTCATA CAGAGTCTCT TACGACTCAA TGACAAGAAG
      421 AAAATCTTCG TCAACATGGT GGAGCACGAC ACACTTGTCT ACTCCAAAAA TATCAAAGAT
      481 ACAGTCTCAG AAGACCAAAG GGCAATTGAG ACTTTTCAAC AAAGGGTAAT ATCCGGAAAC
      541 CTCCTCGGAT TCCATTGCCC AGCTATCTGT CACTTTATTG TGAAGATAGT GGAAAAGGAA
      601 GGTGGCTCCT ACAAATGCCA TCATTGCGAT AAAGGAAAGG CCATCGTTGA AGATGCCTCT
      661 GCCGACAGTG GTCCCAAAGA TGGACCCCCA CCCACGAGGA GCATCGTGGA AAAAGAAGAC
      721 GTTCCAACCA CGTCTTCAAA GCAAGTGGAT TGATGTGATA TCTCCACTGA CGTAAGGGAT
      781 GACGCACAAT CCCACTATCC TTCGCAAGAC CCTTCCTCTA TATAAGGAAG TTCATTTCAT
      841 TTGGAGAGAA CACGGGGGAC TCTAGAGGAT CCCCGGGTGG TCAGTCCCTT ATGTTACGTC
      901 CTGTAGAAAC CCCAACCCGT GAAATCAAAA AACTCGACGG CCTGTGGGCA TTCAGTCTGG
      961 ATCGCGAAAA CTGTGGAATT GATCAGCGTT GGTGGGAAAG CGCGTTACAA GAAAGCCGGG
     1021 CAATTGCTGT GCCAGGCAGT TTTAACGATC AGTTCGCCGA TGCAGATATT CGTAATTATG
     1081 CGGGCAACGT CTGGTATCAG CGCGAAGTCT TTATACCGAA AGGTTGGGCA GGCCAGCGTA
     1141 TCGTGCTGCG TTTCGATGCG GTCACTCATT ACGGCAAAGT GTGGGTCAAT AATCAGGAAG
     1201 TGATGGAGCA TCAGGGCGGC TATACGCCAT TTGAAGCCGA TGTCACGCCG TATGTTATTG
     1261 CCGGGAAAAG TGTACGTATC ACCGTTTGTG TGAACAACGA ACTGAACTGG CAGACTATCC
     1321 CGCCGGGAAT GGTGATTACC GACGAAAACG GCAAGAAAAA GCAGTCTTAC TTCCATGATT
     1381 TCTTTAACTA TGCCGGAATC CATCGCAGCG TAATGCTCTA CACCACGCCG AACACCTGGG
     1441 TGGACGATAT CACCGTGGTG ACGCATGTCG CGCAAGACTG TAACCACGCG TCTGTTGACT
     1501 GGCAGGTGGT GGCCAATGGT GATGTCAGCG TTGAACTGCG TGATGCGGAT CAACAGGTGG
     1561 TTGCAACTGG ACAAGGCACT AGCGGGACTT TGCAAGTGGT GAATCCGCAC CTCTGGCAAC
     1621 CGGGTGAAGG TTATCTCTAT GAACTGTGCG TCACAGCCAA AAGCCAGACA GAGTGTGATA
     1681 TCTACCCGCT TCGCGTCGGC ATCCGGTCAG TGGCAGTGAA GGGCGAACAG TTCCTGATTA
     1741 ACCACAAACC GTTCTACTTT ACTGGCTTTG GTCGTCATGA AGATGCGGAC TTGCGTGGCA
     1801 AAGGATTCGA TAACGTGCTG ATGGTGCACG ACCACGCATT AATGGACTGG ATTGGGGCCA
     1861 ACTCCTACCG TACCTCGCAT TACCCTTACG CTGAAGAGAT GCTCGACTGG GCAGATGAAC
     1921 ATGGCATCGT GGTGATTGAT GAAACTGCTG CTGTCGGCTT TAACCTCTCT TTAGGCATTG
     1981 GTTTCGAAGC GGGCAACAAG CCGAAAGAAC TGTACAGCGA AGAGGCAGTC AACGGGGAAA
     2041 CTCAGCAAGC GCACTTACAG GCGATTAAAG AGCTGATAGC GCGTGACAAA AACCACCCAA
     2101 GCGTGGTGAT GTGGAGTATT GCCAACGAAC CGGATACCCG TCCGCAAGGT GCACGGGAAT
     2161 ATTTCGCGCC ACTGGCGGAA GCAACGCGTA AACTCGACCC GACGCGTCCG ATCACCTGCG
     2221 TCAATGTAAT GTTCTGCGAC GCTCACACCG ATACCATCAG CGATCTCTTT GATGTGCTGT
     2281 GCCTGAACCG TTATTACGGA TGGTATGTCC AAAGCGGCGA TTTGGAAACG GCAGAGAAGG
     2341 TACTGGAAAA AGAACTTCTG GCCTGGCAGG AGAAACTGCA TCAGCCGATT ATCATCACCG
     2401 AATACGGCGT GGATACGTTA GCCGGGCTGC ACTCAATGTA CACCGACATG TGGAGTGAAG
     2461 AGTATCAGTG TGCATGGCTG GATATGTATC ACCGCGTCTT TGATCGCGTC AGCGCCGTCG
     2521 TCGGTGAACA GGTATGGAAT TTCGCCGATT TTGCGACCTC GCAAGGCATA TTGCGCGTTG
     2581 GCGGTAACAA GAAAGGGATC TTCACTCGCG ACCGCAAACC GAAGTCGGCG GCTTTTCTGC
     2641 TGCAAAAACG CTGGACTGGC ATGAACTTCG GTGAAAAACC GCAGCAGGGA GGCAAACAAT
     2701 GAATCAACAA CTCTCCTGGC GCACCATCGT CGGCTACAGC CTCGGGAATT GCTACCGAGC
     2761 TCGAATTTCC CCGATCGTTC AAACATTTGG CAATAAAGTT TCTTAAGATT GAATCCTGTT
     2821 GCCGGTCTTG CGATGATTAT CATATAATTT CTGTTGAATT ACGTTAAGCA TGTAATAATT
     2881 AACATGTAAT GCATGACGTT ATTTATGAGA TGGGTTTTTA TGATTAGAGT CCCGCAATTA
     2941 TACATTTAAT ACGCGATAGA AAACAAAATA TAGCGCGCAA ACTAGGATAA ATTATCGCGC
     3001 GCGGTGTCAT CTATGTTACT AGATCGGGAA TTCCTGCAGC CCGGGGGATC CACTAGTTCT
     3061 AGAGCGGCCG CCACCGCGGT GGAGCTCCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTTC
     3121 GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT
     3181 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG
     3241 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
     3301 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC
     3361 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC
     3421 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
     3481 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
     3541 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     3601 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     3661 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     3721 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     3781 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     3841 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     3901 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     3961 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     4021 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     4081 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     4141 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     4201 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     4261 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     4321 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     4381 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     4441 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     4501 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     4561 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     4621 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     4681 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     4741 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     4801 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     4861 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     4921 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     4981 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     5041 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     5101 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     5161 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     5221 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
     5281 AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
     5341 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
     5401 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
     5461 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
     5521 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
     5581 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC
     5641 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA
     5701 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTCCA TTCGCCATTC
     5761 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
     5821 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
     5881 CGACGTTGTA AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGGT
     5941 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCT
//
