Start: Wed Jun 9 11:25:53 CDT 2021 Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 2 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -p : paired input Considering argument: -k : k-mer size is 31 File name: Algae_6_1P.fastq File name: Algae_6_2P.fastq ERROR CORRECTION Creating read objects... Reading file: Algae_6_1P.fastq Reading file: Algae_6_2P.fastq Finished creating read objects! Constructing k-mers... Processing file 1/2... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 220557240 unique k-mers from the set of 297084286 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 66. Finished preprocessing k-mers! Processing file 2/2... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 199815318 unique k-mers from the set of 326147128 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 43. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting paired files. 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Corrected... Reads with Multiple Corrections: 758687 Substitution Corrections: 2981354 Single Insertion Corrections: 118126 Single Deletion Corrections: 141549 Total Homopolymer Corrections: 348538 Breakdown: < -10: 3 -10 : 5 -9 : 4 -8 : 9 -7 : 20 -6 : 77 -5 : 166 -4 : 1280 -3 : 4802 -2 : 56300 -1 : 153908 1 : 120864 2 : 9558 3 : 1069 4 : 206 5 : 30 6 : 30 7 : 21 8 : 31 9 : 18 10 : 7 > 10: 130 Total Insertions (Single + Homopolymer): 263094 Total Deletions (Single + Homopolymer): 429173 Finished correcting reads! Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 2 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -p : paired input Considering argument: -k : k-mer size is 31 File name: Algae_7_1P.fastq File name: Algae_7_2P.fastq ERROR CORRECTION Creating read objects... Reading file: Algae_7_1P.fastq Reading file: Algae_7_2P.fastq Finished creating read objects! Constructing k-mers... Processing file 1/2... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 216508192 unique k-mers from the set of 315405282 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 73. Finished preprocessing k-mers! Processing file 2/2... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 201551568 unique k-mers from the set of 356648426 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 41. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting paired files. 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Corrected... Reads with Multiple Corrections: 774371 Substitution Corrections: 3080334 Single Insertion Corrections: 132193 Single Deletion Corrections: 159026 Total Homopolymer Corrections: 399861 Breakdown: < -10: 1 -10 : 1 -9 : 1 -8 : 5 -7 : 13 -6 : 100 -5 : 256 -4 : 1591 -3 : 5096 -2 : 62924 -1 : 180356 1 : 137943 2 : 9824 3 : 1065 4 : 354 5 : 48 6 : 20 7 : 24 8 : 13 9 : 20 10 : 15 > 10: 191 Total Insertions (Single + Homopolymer): 295357 Total Deletions (Single + Homopolymer): 488912 Finished correcting reads! Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 2 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -p : paired input Considering argument: -k : k-mer size is 31 File name: Algae_8_1P.fastq File name: Algae_8_2P.fastq ERROR CORRECTION Creating read objects... Reading file: Algae_8_1P.fastq Reading file: Algae_8_2P.fastq Finished creating read objects! Constructing k-mers... Processing file 1/2... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 226583568 unique k-mers from the set of 340690070 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 67. Finished preprocessing k-mers! Processing file 2/2... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 196569476 unique k-mers from the set of 373453770 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 50. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting paired files. 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Corrected... Reads with Multiple Corrections: 815515 Substitution Corrections: 3305554 Single Insertion Corrections: 147031 Single Deletion Corrections: 179612 Total Homopolymer Corrections: 447272 Breakdown: < -10: 3 -10 : 4 -9 : 2 -8 : 2 -7 : 21 -6 : 129 -5 : 287 -4 : 1878 -3 : 5634 -2 : 69505 -1 : 201614 1 : 155255 2 : 10599 3 : 1430 4 : 476 5 : 70 6 : 34 7 : 43 8 : 22 9 : 32 10 : 20 > 10: 212 Total Insertions (Single + Homopolymer): 331197 Total Deletions (Single + Homopolymer): 547080 Finished correcting reads! Finish: Wed Jun 9 14:20:50 CDT 2021