Start: Wed Jun 9 14:34:03 CDT 2021 Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 1 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -k : k-mer size is 31 File name: Algae_6_1U.fastq ERROR CORRECTION Creating read objects... Reading file: Algae_6_1U.fastq Finished creating read objects! Constructing k-mers... Processing file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 83934822 unique k-mers from the set of 97155762 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 31. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Corrected... Reads with Multiple Corrections: 47084 Substitution Corrections: 196533 Single Insertion Corrections: 6442 Single Deletion Corrections: 9722 Total Homopolymer Corrections: 21195 Breakdown: < -10: 0 -10 : 2 -9 : 0 -8 : 2 -7 : 1 -6 : 11 -5 : 17 -4 : 139 -3 : 330 -2 : 5485 -1 : 10277 1 : 4484 2 : 376 3 : 43 4 : 17 5 : 3 6 : 1 7 : 2 8 : 0 9 : 0 10 : 0 > 10: 5 Total Insertions (Single + Homopolymer): 11910 Total Deletions (Single + Homopolymer): 32709 Finished correcting reads! Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 1 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -k : k-mer size is 31 File name: Algae_6_2U.fastq ERROR CORRECTION Creating read objects... Reading file: Algae_6_2U.fastq Finished creating read objects! Constructing k-mers... Processing file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 111389336 unique k-mers from the set of 129004382 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 35. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Corrected... Reads with Multiple Corrections: 58070 Substitution Corrections: 238571 Single Insertion Corrections: 7334 Single Deletion Corrections: 11413 Total Homopolymer Corrections: 24854 Breakdown: < -10: 0 -10 : 1 -9 : 1 -8 : 3 -7 : 4 -6 : 8 -5 : 17 -4 : 169 -3 : 364 -2 : 5932 -1 : 11322 1 : 6420 2 : 487 3 : 55 4 : 29 5 : 6 6 : 7 7 : 1 8 : 5 9 : 4 10 : 4 > 10: 15 Total Insertions (Single + Homopolymer): 15204 Total Deletions (Single + Homopolymer): 36571 Finished correcting reads! Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 1 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -k : k-mer size is 31 File name: Algae_7_1U.fastq ERROR CORRECTION Creating read objects... Reading file: Algae_7_1U.fastq Finished creating read objects! Constructing k-mers... Processing file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 77938190 unique k-mers from the set of 92678666 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 40. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Corrected... Reads with Multiple Corrections: 41949 Substitution Corrections: 177840 Single Insertion Corrections: 5753 Single Deletion Corrections: 9184 Total Homopolymer Corrections: 20347 Breakdown: < -10: 0 -10 : 1 -9 : 0 -8 : 3 -7 : 2 -6 : 9 -5 : 22 -4 : 160 -3 : 254 -2 : 5239 -1 : 10039 1 : 4045 2 : 489 3 : 41 4 : 11 5 : 7 6 : 3 7 : 2 8 : 0 9 : 1 10 : 1 > 10: 18 Total Insertions (Single + Homopolymer): 11029 Total Deletions (Single + Homopolymer): 31315 Finished correcting reads! Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 1 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -k : k-mer size is 31 File name: Algae_7_2U.fastq ERROR CORRECTION Creating read objects... Reading file: Algae_7_2U.fastq Finished creating read objects! Constructing k-mers... Processing file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% Removed 87071148 unique k-mers from the set of 101882340 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 36. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Corrected... Reads with Multiple Corrections: 38467 Substitution Corrections: 162221 Single Insertion Corrections: 4699 Single Deletion Corrections: 8063 Total Homopolymer Corrections: 16753 Breakdown: < -10: 0 -10 : 0 -9 : 2 -8 : 1 -7 : 0 -6 : 12 -5 : 18 -4 : 145 -3 : 304 -2 : 4623 -1 : 7716 1 : 3516 2 : 309 3 : 49 4 : 20 5 : 2 6 : 6 7 : 7 8 : 0 9 : 3 10 : 3 > 10: 17 Total Insertions (Single + Homopolymer): 9212 Total Deletions (Single + Homopolymer): 26705 Finished correcting reads! Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 1 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -k : k-mer size is 31 File name: Algae_8_1U.fastq ERROR CORRECTION Creating read objects... Reading file: Algae_8_1U.fastq Finished creating read objects! Constructing k-mers... Processing file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 71783158 unique k-mers from the set of 87604314 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 33. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Corrected... Reads with Multiple Corrections: 31013 Substitution Corrections: 136359 Single Insertion Corrections: 4507 Single Deletion Corrections: 8098 Total Homopolymer Corrections: 17469 Breakdown: < -10: 0 -10 : 0 -9 : 1 -8 : 0 -7 : 2 -6 : 9 -5 : 19 -4 : 111 -3 : 237 -2 : 4518 -1 : 9165 1 : 3127 2 : 206 3 : 49 4 : 12 5 : 4 6 : 2 7 : 2 8 : 1 9 : 0 10 : 1 > 10: 3 Total Insertions (Single + Homopolymer): 8305 Total Deletions (Single + Homopolymer): 27626 Finished correcting reads! Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 1 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -k : k-mer size is 31 File name: Algae_8_2U.fastq ERROR CORRECTION Creating read objects... Reading file: Algae_8_2U.fastq Finished creating read objects! Constructing k-mers... Processing file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 73000536 unique k-mers from the set of 86246752 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 21. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting file 1/1... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% Corrected... Reads with Multiple Corrections: 28517 Substitution Corrections: 123512 Single Insertion Corrections: 3772 Single Deletion Corrections: 6958 Total Homopolymer Corrections: 14763 Breakdown: < -10: 0 -10 : 1 -9 : 1 -8 : 5 -7 : 2 -6 : 14 -5 : 23 -4 : 133 -3 : 262 -2 : 4236 -1 : 7170 1 : 2552 2 : 235 3 : 49 4 : 36 5 : 8 6 : 6 7 : 4 8 : 3 9 : 3 10 : 4 > 10: 16 Total Insertions (Single + Homopolymer): 7280 Total Deletions (Single + Homopolymer): 24190 Finished correcting reads! Finish: Wed Jun 9 14:59:10 CDT 2021