FastQCFastQC Report
Mon 7 Jun 2021
AB2kb_R1.illumina-1.8+_valid_1P.fq.corrected.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAB2kb_R1.illumina-1.8+_valid_1P.fq.corrected.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30984188
Sequences flagged as poor quality0
Sequence length34-125
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGGC111050.022333.133102-103
GGCCCCA147850.016774.395106-107
GTGGCCC148650.016684.12104-105
AGTGGCC99052.6133199E-512519.405102-103
CCCCAGC232500.010667.072108-109
TGGCCCC141505.3333053E-58763.584104-105
CCCAGCT212351.2011247E-45839.638108-109
GCCCCAG239851.5323678E-45170.094106-107
GTTTGGG94258.5167086E-42923.7773100-101
AAGTTTG101959.964998E-42702.953100-101
CTACATG111850.00119940722463.7107100-101
AGAAGTG117750.00132926542340.2634100-101
ACATGTT118400.00134397982327.4158100-101
GAAGTGG216350.0068538211042.1221100-101
CGAGCGT265000.00673071571039.8718100-101
AATCCGC180652.386993E-4442.862198-99
AGAGAGA3892250.0052823666141.59729100-101
TTTATTT126650.004007611460.25312896-97
GTCTGAA138100.005645268655.2574996-97
GAGAGAG3948500.003647477431.40552598-99
Produced by FastQC (version 0.11.5)