FastQCFastQC Report
Mon 7 Jun 2021
AB2kb_R2.illumina-1.8+_valid_2P.fq.corrected.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAB2kb_R2.illumina-1.8+_valid_2P.fq.corrected.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30984188
Sequences flagged as poor quality0
Sequence length33-127
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGT77501.567886E-516163.028108-109
GGGGGTT84751.1249212E-47390.176108-109
GGGGGGG170802.0765583E-87333.9272104-105
GTCTTGG123251.1895752E-46775.5767102-103
AAGTCTT80303.534394E-44456.9824100-101
AGTCTTG72757.5973826E-43130.6086100-101
TGTCCCA122758.2585233E-42915.647100-101
TAACTAG14700.00140137662400.373398-99
ACTAGAC20800.00155583782272.559698-99
CTGTGCA169300.00157089232113.9731100-101
TCTCTCC182950.00183438041956.2484100-101
TTAACTA26600.00254429041777.03998-99
CTCTCCG159500.00365114541427.911100-101
TGGCCTG265500.00386283311348.0061100-101
CTTAGAA39750.0056806781189.163398-99
TAGGAAC42000.00634178151125.45898-99
TCTCTCT2721500.0920.5473112-113
CTCTCTC2755106.2358595E-8454.66037110-111
ACATTGA120300.002295631269.3402396-97
TCATTTT127100.006231223753.9043296-97
Produced by FastQC (version 0.11.5)