Basic Statistics
Measure | Value |
---|---|
Filename | AB4kb_R1.illumina-1.8+_valid_1P.fq.corrected.fq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14041228 |
Sequences flagged as poor quality | 0 |
Sequence length | 33-124 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG | 31912 | 0.22727356894995224 | No Hit |
CACACACACACACACACACACACACACACACACACACACACACACACACA | 20545 | 0.14631911112048035 | No Hit |
ACACACACACACACACACACACACACACACACACACACACACACACACAC | 16666 | 0.11869332226497567 | No Hit |
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA | 15094 | 0.10749772028486397 | No Hit |
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG | 14485 | 0.10316049280020237 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATTGTT | 5630 | 0.0 | 20583.457 | 106-107 |
CTGATTG | 7400 | 0.0 | 15660.115 | 104-105 |
TTGTTCC | 7490 | 0.0 | 15471.944 | 108 |
ATTGTTC | 6130 | 4.5844525E-5 | 9452.272 | 106-107 |
TGATTGT | 6985 | 5.9524995E-5 | 8295.266 | 104-105 |
CACATAC | 8935 | 2.9218275E-4 | 4323.2554 | 102-103 |
GCTGATT | 6720 | 3.3053904E-4 | 4311.193 | 102-103 |
ATCATGC | 10450 | 3.9966332E-4 | 3696.4866 | 102-103 |
TGCTGAT | 14175 | 7.3535746E-4 | 2725.0994 | 102-103 |
TTATCAT | 4560 | 0.0016738873 | 2117.7788 | 100-101 |
TGTATGA | 4720 | 0.0017933975 | 2045.9897 | 100-101 |
ATCGGAA | 5210 | 0.0021850218 | 1853.5646 | 100-101 |
TATCATG | 5050 | 0.0037321053 | 1434.2186 | 100-101 |
CGCACAT | 7440 | 0.004455234 | 1297.9934 | 100-101 |
GCACCCT | 7445 | 0.004461222 | 1297.1218 | 100-101 |
ACATGAT | 8430 | 0.005719459 | 1145.5602 | 100-101 |
GCACATA | 6815 | 0.006795808 | 1062.7739 | 100-101 |
AGAGGAA | 9825 | 0.0077683735 | 982.9081 | 100-101 |
TCCCGTT | 4285 | 3.611838E-5 | 832.13257 | 98-99 |
CACACAA | 11035 | 6.1520044E-4 | 323.12534 | 98-99 |