FastQCFastQC Report
Mon 7 Jun 2021
AB4kb_R1.illumina-1.8+_valid_1P.fq.corrected.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAB4kb_R1.illumina-1.8+_valid_1P.fq.corrected.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14041228
Sequences flagged as poor quality0
Sequence length33-124
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG319120.22727356894995224No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA205450.14631911112048035No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC166660.11869332226497567No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA150940.10749772028486397No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG144850.10316049280020237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGTT56300.020583.457106-107
CTGATTG74000.015660.115104-105
TTGTTCC74900.015471.944108
ATTGTTC61304.5844525E-59452.272106-107
TGATTGT69855.9524995E-58295.266104-105
CACATAC89352.9218275E-44323.2554102-103
GCTGATT67203.3053904E-44311.193102-103
ATCATGC104503.9966332E-43696.4866102-103
TGCTGAT141757.3535746E-42725.0994102-103
TTATCAT45600.00167388732117.7788100-101
TGTATGA47200.00179339752045.9897100-101
ATCGGAA52100.00218502181853.5646100-101
TATCATG50500.00373210531434.2186100-101
CGCACAT74400.0044552341297.9934100-101
GCACCCT74450.0044612221297.1218100-101
ACATGAT84300.0057194591145.5602100-101
GCACATA68150.0067958081062.7739100-101
AGAGGAA98250.0077683735982.9081100-101
TCCCGTT42853.611838E-5832.1325798-99
CACACAA110356.1520044E-4323.1253498-99
Produced by FastQC (version 0.11.5)