FastQCFastQC Report
Mon 7 Jun 2021
AB4kb_R2.illumina-1.8+_valid_2P.fq.corrected.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAB4kb_R2.illumina-1.8+_valid_2P.fq.corrected.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14041228
Sequences flagged as poor quality0
Sequence length32-132
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT262710.18709902011419513No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC225960.16092609563778892No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG154580.11009008613776516No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA150400.10711313853745555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTTAA35400.033004.523116
GAAGTTA34601.43687375E-516883.818114-115
TGAAGTT94450.012370.145114-115
TTACCTC37455.0498915E-510399.29106-107
CTTGAAG116650.010015.947112-113
CGCTTGA66505.3077412E-58784.663110-111
TCTTACC35158.89715E-58309.816104-105
TTGAAGT99951.1990354E-45844.7236112-113
TACCTCG35752.300776E-45446.901106-107
TATTCGA26902.4315827E-45429.183102-103
TCCTTCA117701.6627209E-44963.2974110-111
ACCTCGA84902.595267E-44587.201108-109
CTTACCT40154.062628E-44157.126104-105
TCTCTTA40255.443728E-43628.4478102-103
CGCGCTT109654.3289026E-43551.7866108-109
GCTTGAA93706.3219405E-43117.2898110-111
AGGTCGC93806.335442E-43113.9663104-105
TCCTTCC135106.571506E-42882.7046106-107
CTTCCTT146607.7378325E-42656.5713108-109
ACAATCC58650.00115577492490.1113102-103
Produced by FastQC (version 0.11.5)