Basic Statistics
Measure | Value |
---|---|
Filename | AB4kb_R2.illumina-1.8+_valid_2P.fq.corrected.fq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14041228 |
Sequences flagged as poor quality | 0 |
Sequence length | 32-132 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT | 26271 | 0.18709902011419513 | No Hit |
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC | 22596 | 0.16092609563778892 | No Hit |
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG | 15458 | 0.11009008613776516 | No Hit |
CACACACACACACACACACACACACACACACACACACACACACACACACA | 15040 | 0.10711313853745555 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGTTAA | 3540 | 0.0 | 33004.523 | 116 |
GAAGTTA | 3460 | 1.43687375E-5 | 16883.818 | 114-115 |
TGAAGTT | 9445 | 0.0 | 12370.145 | 114-115 |
TTACCTC | 3745 | 5.0498915E-5 | 10399.29 | 106-107 |
CTTGAAG | 11665 | 0.0 | 10015.947 | 112-113 |
CGCTTGA | 6650 | 5.3077412E-5 | 8784.663 | 110-111 |
TCTTACC | 3515 | 8.89715E-5 | 8309.816 | 104-105 |
TTGAAGT | 9995 | 1.1990354E-4 | 5844.7236 | 112-113 |
TACCTCG | 3575 | 2.300776E-4 | 5446.901 | 106-107 |
TATTCGA | 2690 | 2.4315827E-4 | 5429.183 | 102-103 |
TCCTTCA | 11770 | 1.6627209E-4 | 4963.2974 | 110-111 |
ACCTCGA | 8490 | 2.595267E-4 | 4587.201 | 108-109 |
CTTACCT | 4015 | 4.062628E-4 | 4157.126 | 104-105 |
TCTCTTA | 4025 | 5.443728E-4 | 3628.4478 | 102-103 |
CGCGCTT | 10965 | 4.3289026E-4 | 3551.7866 | 108-109 |
GCTTGAA | 9370 | 6.3219405E-4 | 3117.2898 | 110-111 |
AGGTCGC | 9380 | 6.335442E-4 | 3113.9663 | 104-105 |
TCCTTCC | 13510 | 6.571506E-4 | 2882.7046 | 106-107 |
CTTCCTT | 14660 | 7.7378325E-4 | 2656.5713 | 108-109 |
ACAATCC | 5865 | 0.0011557749 | 2490.1113 | 102-103 |