Start: Sun Jun 6 08:21:38 CDT 2021 Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 2 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -p : paired input Considering argument: -k : k-mer size is 31 File name: AB2kb_R1.illumina-1.8+_valid_1P.fq File name: AB2kb_R2.illumina-1.8+_valid_2P.fq ERROR CORRECTION Creating read objects... Reading file: AB2kb_R1.illumina-1.8+_valid_1P.fq Reading file: AB2kb_R2.illumina-1.8+_valid_2P.fq Finished creating read objects! Constructing k-mers... Processing file 1/2... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% Removed 312444148 unique k-mers from the set of 852210866 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 94. Finished preprocessing k-mers! Processing file 2/2... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% Removed 349008822 unique k-mers from the set of 1211837765 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 132. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting paired files. 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Corrected... Reads with Multiple Corrections: 5652681 Substitution Corrections: 22422674 Single Insertion Corrections: 1370071 Single Deletion Corrections: 1477614 Total Homopolymer Corrections: 3236775 Breakdown: < -10: 69 -10 : 33 -9 : 38 -8 : 96 -7 : 168 -6 : 532 -5 : 1390 -4 : 7902 -3 : 41697 -2 : 560950 -1 : 1486393 1 : 1044447 2 : 83292 3 : 7065 4 : 1484 5 : 255 6 : 156 7 : 123 8 : 83 9 : 57 10 : 54 > 10: 491 Total Insertions (Single + Homopolymer): 2613022 Total Deletions (Single + Homopolymer): 4255364 Finished correcting reads! Pollux 1.0.2 Source compiled on Jan 25 2018 at 17:47:52. Considering argument: -i : number of input files is 2 Considering argument: -o : output directory is ../reads.Trimmomatic2.pollux Considering argument: -p : paired input Considering argument: -k : k-mer size is 31 File name: AB4kb_R1.illumina-1.8+_valid_1P.fq File name: AB4kb_R2.illumina-1.8+_valid_2P.fq ERROR CORRECTION Creating read objects... Reading file: AB4kb_R1.illumina-1.8+_valid_1P.fq Reading file: AB4kb_R2.illumina-1.8+_valid_2P.fq Finished creating read objects! Constructing k-mers... Processing file 1/2... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 348291296 unique k-mers from the set of 599165646 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 53. Finished preprocessing k-mers! Processing file 2/2... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Preprocessing k-mers... 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Removed 328222770 unique k-mers from the set of 735194016 total k-mers. Resizing... Finished resizing... Low k-mer count value was observed to be 80. Finished preprocessing k-mers! Finished constructing k-mers! Correcting reads... Correcting paired files. 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% 95% 100% Corrected... Reads with Multiple Corrections: 3468449 Substitution Corrections: 14148005 Single Insertion Corrections: 764409 Single Deletion Corrections: 899356 Total Homopolymer Corrections: 2628889 Breakdown: < -10: 159 -10 : 55 -9 : 51 -8 : 153 -7 : 219 -6 : 790 -5 : 2133 -4 : 11556 -3 : 43143 -2 : 516567 -1 : 1243599 1 : 732259 2 : 66050 3 : 7680 4 : 2548 5 : 381 6 : 255 7 : 168 8 : 149 9 : 118 10 : 103 > 10: 753 Total Insertions (Single + Homopolymer): 1669895 Total Deletions (Single + Homopolymer): 3370913 Finished correcting reads! Finish: Mon Jun 7 15:18:30 CDT 2021