FastQCFastQC Report
Fri 21 May 2021
AB2kb_R1.illumina-1.8+.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAB2kb_R1.illumina-1.8+.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39389202
Sequences flagged as poor quality0
Sequence length100
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG410130.1041224445217245No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)