Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
RID: 991617086-13184-28031
Query= gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE
PROTEIN
(197 letters)
Database: nr
693,878 sequences; 218,617,282 total letters
If you have any problems or questions with the results of this search
please refer to the BLAST FAQs
Taxonomy reports
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAA02945.1| (D13793) ORF [Bacillus subtilis] 397 e-110
dbj|BAA02944.1| (D13793) ORF [Bacillus subtilis] >gi|226067... 397 e-110
dbj|BAA02946.1| (D13793) ORF [Bacillus subtilis] 397 e-110
sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN >gi|742... 396 e-109
pir||T36076 tmrB-like protein - Streptomyces coelicolor >gi... 144 1e-33
pir||F75399 antibiotic resistance protein - Deinococcus rad... 114 1e-24
emb|CAA31896.1| (X13543) unidentified peptide (AA 1-92) (13... 90 3e-17
emb|CAB95294.1| (AL359779) putative bifunctional protein (A... 87 2e-16
pir||S06033 hypothetical protein - Enterobacteriaceae plasm... 86 5e-16
pir||T36817 ATP/GTP-binding protein - Streptomyces coelicol... 86 5e-16
pir||S06031 hypothetical protein - Enterobacteriaceae plasm... 83 4e-15
pir||S06034 hypothetical protein - Klebsiella pneumoniae pl... 82 9e-15
Alignments
>dbj|BAA02945.1| (D13793) ORF [Bacillus subtilis]
Length = 199
Score = 397 bits (1021), Expect = e-110
Identities = 197/197 (100%), Positives = 197/197 (100%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA
Sbjct: 3 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 62
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR
Sbjct: 63 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 122
Query: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL
Sbjct: 123 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 182
Query: 181 RRFADRLMVKLNHIRIK 197
RRFADRLMVKLNHIRIK
Sbjct: 183 RRFADRLMVKLNHIRIK 199
>dbj|BAA02944.1| (D13793) ORF [Bacillus subtilis]
prf||1410166A tunicamycin resistance gene tmrB [Escherichia coli]
Length = 206
Score = 397 bits (1021), Expect = e-110
Identities = 197/197 (100%), Positives = 197/197 (100%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA
Sbjct: 10 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 69
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR
Sbjct: 70 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 129
Query: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL
Sbjct: 130 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 189
Query: 181 RRFADRLMVKLNHIRIK 197
RRFADRLMVKLNHIRIK
Sbjct: 190 RRFADRLMVKLNHIRIK 206
>dbj|BAA02946.1| (D13793) ORF [Bacillus subtilis]
Length = 197
Score = 397 bits (1021), Expect = e-110
Identities = 197/197 (100%), Positives = 197/197 (100%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA
Sbjct: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR
Sbjct: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
Query: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL
Sbjct: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
Query: 181 RRFADRLMVKLNHIRIK 197
RRFADRLMVKLNHIRIK
Sbjct: 181 RRFADRLMVKLNHIRIK 197
>sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN
pir||YTBST tunicamycin resistance protein - Bacillus subtilis
dbj|BAA08948.1| (D50453) tunicamycin resistance protein [Bacillus subtilis]
emb|CAB12108.1| (Z99105) ATP-binding membrane protein [Bacillus subtilis]
Length = 197
Score = 396 bits (1018), Expect = e-109
Identities = 196/197 (99%), Positives = 197/197 (99%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MIIWINGAFGSGKTQTAFELHRRLNPSYVYDP+KMGFALRSMVPQEIAKDDFQSYPLWRA
Sbjct: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPQKMGFALRSMVPQEIAKDDFQSYPLWRA 60
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR
Sbjct: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
Query: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL
Sbjct: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
Query: 181 RRFADRLMVKLNHIRIK 197
RRFADRLMVKLNHIRIK
Sbjct: 181 RRFADRLMVKLNHIRIK 197
>pir||T36076 tmrB-like protein - Streptomyces coelicolor
emb|CAB40928.1| (AL049630) TmrB-like protein [Streptomyces coelicolor A3(2)]
Length = 213
Score = 144 bits (363), Expect = 1e-33
Identities = 78/197 (39%), Positives = 112/197 (56%), Gaps = 18/197 (9%)
Query: 17 AFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRAFNYSLLASLTDTYRGI 76
A E+ RRL S V DPE GF LR M+P E+ ++DFQ WR +L + G+
Sbjct: 17 AHEIRRRLPGSVVCDPEHAGFGLRRMLPPEL-REDFQDLVSWRQGVVEVLDLALTRHDGV 75
Query: 77 LIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLRTRAEG----------- 125
+I PMT+ P YF E +GRLR+ G VHHF+L+A++ET+L+RLR R G
Sbjct: 76 VIAPMTVTDPGYFAETVGRLRELGHDVHHFSLLAARETVLRRLRERGPGHALRYVTGKAG 135
Query: 126 ------KNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGS 179
+ SWA +Q+D C+E L+ P F +H+ TD+ ++ A+ IA A L L P+ G+
Sbjct: 136 QGSGPRRESWAVRQLDHCLERLAEPEFAEHLWTDHTTVPKTADRIAVLAGLTLKPNNEGA 195
Query: 180 LRRFADRLMVKLNHIRI 196
LR + V + HIR+
Sbjct: 196 LRTRMRQAGVGIRHIRV 212
>pir||F75399 antibiotic resistance protein - Deinococcus radiodurans (strain
R1)
gb|AAF10983.1|AE001986_8 (AE001986) antibiotic resistance protein [Deinococcus radiodurans]
Length = 195
Score = 114 bits (286), Expect = 1e-24
Identities = 70/179 (39%), Positives = 95/179 (52%), Gaps = 4/179 (2%)
Query: 2 IIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRAF 61
IIWING FG GKT TA LH RL S+V++PE+MG ALR + P D Q +P+W
Sbjct: 8 IIWINGPFGVGKTHTAHTLHERLPGSFVFEPEEMGQALRKLTPG--FSGDPQEHPMWIPL 65
Query: 62 NYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLRT 121
L + G LIVP++I ++ L+ G VHHFTL+A +L+RL
Sbjct: 66 MLDALQYASREAAGPLIVPVSISDTARHRRLMSGLKDRGLSVHHFTLIAPLNVVLERL-- 123
Query: 122 RAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
R +G+ ++ + L F+ HI T L Q VAE IAA+ L L P +G+L
Sbjct: 124 RRDGQPQVNVGTVEDRLNELRGEQFQTHIDTAGLGTQQVAEQIAAQVGLTLAPPPQGAL 182
>emb|CAA31896.1| (X13543) unidentified peptide (AA 1-92) (1336 is 2nd base in
codon) [Enterobacteriaceae]
Length = 92
Score = 90.1 bits (222), Expect = 3e-17
Identities = 41/93 (44%), Positives = 58/93 (62%), Gaps = 1/93 (1%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MIIWING FG+GKT A L R + S ++DPE++GF ++ VP A D+Q PLWR
Sbjct: 1 MIIWINGPFGAGKTTLAERLRDRRSKSLIFDPEEIGFVVKETVPMP-ASGDYQDLPLWRG 59
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEII 93
+ + + Y +I+PMT+VHP+Y EI+
Sbjct: 60 LTIAAVREIRRNYSQDIIIPMTLVHPDYLTEIL 92
>emb|CAB95294.1| (AL359779) putative bifunctional protein (ATP/GTP binding
protein/MutT-like) [Streptomyces coelicolor A3(2)]
Length = 347
Score = 87.4 bits (215), Expect = 2e-16
Identities = 48/123 (39%), Positives = 70/123 (56%), Gaps = 2/123 (1%)
Query: 2 IIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKD--DFQSYPLWR 59
++WINGAFG+GKT TA EL + S ++DPE +G AL ++P + + DFQ P+WR
Sbjct: 3 VVWINGAFGAGKTTTARELIELIPNSTLFDPEVVGGALAHLLPPKRLAEVGDFQDLPIWR 62
Query: 60 AFNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRL 119
A+L G L+VPMT++ EY +EI G L V H L ++ L +R+
Sbjct: 63 RLVIDTAAALLADLGGTLVVPMTLLRQEYRDEIFGGLAARRIEVRHILLAPAETILRERI 122
Query: 120 RTR 122
+R
Sbjct: 123 ASR 125
>pir||S06033 hypothetical protein - Enterobacteriaceae plasmid pWP14a
(fragment)
emb|CAA31894.1| (X13542) hypothetical protein [Plasmid pWP14a]
Length = 92
Score = 86.3 bits (212), Expect = 5e-16
Identities = 40/93 (43%), Positives = 58/93 (62%), Gaps = 1/93 (1%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MIIWING FG+GKT A L R S ++DPE++GF ++ VP A D+Q PLWR
Sbjct: 1 MIIWINGPFGAGKTTLAERLRDRRPKSLIFDPEEIGFVVKETVPIP-ASGDYQDLPLWRG 59
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEII 93
+ ++ + Y +I+PMT+V+P+Y EI+
Sbjct: 60 LTIAAVSEIRRNYSQDIIIPMTLVYPDYLTEIL 92
>pir||T36817 ATP/GTP-binding protein - Streptomyces coelicolor
emb|CAA20793.1| (AL031541) ATP/GTP binding protein [Streptomyces coelicolor A3(2)]
Length = 174
Score = 86.3 bits (212), Expect = 5e-16
Identities = 47/119 (39%), Positives = 64/119 (53%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MI+W+NG G+GKT T + R L S V+D EK+G L + P D+FQ + WR
Sbjct: 1 MIVWLNGTHGAGKTTTGALVQRLLPDSRVFDAEKVGETLMDITPGLPDTDNFQHWAPWRP 60
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRL 119
+ D G L++PMT++ EY+ EI G L G V HF L A +ETL R+
Sbjct: 61 LVVETARRVLDYTGGTLVMPMTVLVEEYWREIAGGLAAHGVPVRHFVLHADQETLRGRI 119
>pir||S06031 hypothetical protein - Enterobacteriaceae plasmid pWP113a
(fragment)
Length = 86
Score = 83.2 bits (204), Expect = 4e-15
Identities = 38/87 (43%), Positives = 54/87 (61%), Gaps = 1/87 (1%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MIIWING FG+GKT A L R + S ++DPE++GF ++ VP A D+Q PLWR
Sbjct: 1 MIIWINGPFGAGKTTLAERLRDRRSKSLIFDPEEIGFVVKETVPMP-ASGDYQDLPLWRG 59
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPE 87
+ + + Y +I+PMT+VHP+
Sbjct: 60 LTIAAVREIRRNYSQDIIIPMTLVHPD 86
>pir||S06034 hypothetical protein - Klebsiella pneumoniae plasmid pWP116a
(fragment)
emb|CAA31892.1| (X13541) hypothetical protein [Plasmid pWP116a]
Length = 92
Score = 82.0 bits (201), Expect = 9e-15
Identities = 38/88 (43%), Positives = 55/88 (62%), Gaps = 1/88 (1%)
Query: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
MIIWING FG+GKT A L R S ++DPE++GF ++ VP A D+Q PLWR
Sbjct: 1 MIIWINGPFGAGKTTLAERLRDRRPKSLIFDPEEIGFVVKETVPIP-ASGDYQDLPLWRG 59
Query: 61 FNYSLLASLTDTYRGILIVPMTIVHPEY 88
+ ++ + Y +I+PMT+V+P+Y
Sbjct: 60 LTIAAVSEIRRNYSQDIIIPMTLVYPDY 87
Database: nr
Posted date: May 30, 2001 5:08 AM
Number of letters in database: 218,617,282
Number of sequences in database: 693,878
Lambda K H
0.323 0.138 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83284981
Number of Sequences: 693878
Number of extensions: 3200855
Number of successful extensions: 6684
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 6663
Number of HSP's gapped (non-prelim): 12
length of query: 197
length of database: 218,617,282
effective HSP length: 0
effective length of query: 197
effective length of database: 218,617,282
effective search space: 43067604554
effective search space used: 43067604554
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 72 (32.3 bits)