LOCUS NC_005816 9609 bp DNA circular BCT 21-JUL-2008 DEFINITION Yersinia pestis biovar Microtus str. 91001 plasmid pPCP1, complete sequence. ACCESSION NC_005816 VERSION NC_005816.1 GI:45478711 PROJECT GenomeProject:10638 KEYWORDS . SOURCE Yersinia pestis biovar Microtus str. 91001 ORGANISM Yersinia pestis biovar Microtus str. 91001 Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Yersinia. REFERENCE 1 (bases 1 to 9609) AUTHORS Zhou,D., Tong,Z., Song,Y., Han,Y., Pei,D., Pang,X., Zhai,J., Li,M., Cui,B., Qi,Z., Jin,L., Dai,R., Du,Z., Wang,J., Guo,Z., Wang,J., Huang,P. and Yang,R. TITLE Genetics of metabolic variations between Yersinia pestis biovars and the proposal of a new biovar, microtus JOURNAL J. Bacteriol. 186 (15), 5147-5152 (2004) PUBMED 15262951 REFERENCE 2 (bases 1 to 9609) AUTHORS Song,Y., Tong,Z., Wang,J., Wang,L., Guo,Z., Han,Y., Zhang,J., Pei,D., Zhou,D., Qin,H., Pang,X., Han,Y., Zhai,J., Li,M., Cui,B., Qi,Z., Jin,L., Dai,R., Chen,F., Li,S., Ye,C., Du,Z., Lin,W., Wang,J., Yu,J., Yang,H., Wang,J., Huang,P. and Yang,R. TITLE Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans JOURNAL DNA Res. 11 (3), 179-197 (2004) PUBMED 15368893 REFERENCE 3 (bases 1 to 9609) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (16-MAR-2004) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 4 (bases 1 to 9609) AUTHORS Song,Y., Tong,Z., Wang,L., Han,Y., Zhang,J., Pei,D., Wang,J., Zhou,D., Han,Y., Pang,X., Zhai,J., Chen,F., Qin,H., Wang,J., Li,S., Guo,Z., Ye,C., Du,Z., Lin,W., Wang,J., Yu,J., Yang,H., Wang,J., Huang,P. and Yang,R. TITLE Direct Submission JOURNAL Submitted (24-APR-2003) The Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie Street, Fengtai District, Beijing 100071, People's Republic of China COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017046. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..9609 /organism="Yersinia pestis biovar Microtus str. 91001" /mol_type="genomic DNA" /strain="91001" /db_xref="taxon:229193" /plasmid="pPCP1" /biovar="Microtus" repeat_region 1..1954 /mobile_element="insertion sequence:IS100" gene 87..1109 /locus_tag="YP_pPCP01" /db_xref="GeneID:2767718" CDS 87..1109 /locus_tag="YP_pPCP01" /note="similar to corresponding CDS from previously sequenced pPCP plasmid of Yersinia pestis KIM (AF053945) and CO92 (AL109969), also many transposase entries for insertion sequence IS100 of Yersinia pestis. Contains IS21-like element transposase, HTH domain (Interpro|IPR007101)" /codon_start=1 /transl_table=11 /product="putative transposase" /protein_id="NP_995567.1" /db_xref="GI:45478712" /db_xref="GeneID:2767718" /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS FCRGVA" gene 1106..1888 /locus_tag="YP_pPCP02" /db_xref="GeneID:2767716" CDS 1106..1888 /locus_tag="YP_pPCP02" /note="similar to corresponding CDS form previously sequenced pPCP plasmid of Yersinia pestis KIM (AF053945) and CO92 (AL109969), also many ATP-binding protein entries for insertion sequence IS100 of Yersinia pestis. Contains Chaperonin clpA/B (Interpro|IPR001270). Contains ATP/GTP-binding site motif A (P-loop) (Interpro|IPR001687, Molecular Function: ATP binding (GO:0005524)). Contains Bacterial chromosomal replication initiator protein, DnaA (Interpro|IPR001957, Molecular Function: DNA binding (GO:0003677), Molecular Function: DNA replication origin binding (GO:0003688), Molecular Function: ATP binding (GO:0005524), Biological Process: DNA replication initiation (GO:0006270), Biological Process: regulation of DNA replication (GO:0006275)). Contains AAA ATPase (Interpro|IPR003593, Molecular Function: nucleotide binding (GO:0000166))" /codon_start=1 /transl_table=11 /product="transposase/IS protein" /protein_id="NP_995568.1" /db_xref="GI:45478713" /db_xref="GeneID:2767716" /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE" gene 2925..3119 /gene="rop" /locus_tag="YP_pPCP03" /gene_synonym="rom" /db_xref="GeneID:2767717" CDS 2925..3119 /gene="rop" /locus_tag="YP_pPCP03" /gene_synonym="rom" /note="Best Blastp hit =gi|16082682|ref|NP_395229.1| (NC_003132) putative replication regulatory protein [Yersinia pestis], gi|5763813|emb|CAB531 66.1| (AL109969) putative replication regulatory protein [Yersinia pestis]; similar to gb|AAK91579.1| (AY048853), RNAI modulator protein Rom [Salmonella choleraesuis], Contains Regulatory protein Rop (Interpro|IPR000769)" /codon_start=1 /transl_table=11 /product="putative replication regulatory protein" /protein_id="NP_995569.1" /db_xref="GI:45478714" /db_xref="GeneID:2767717" /translation="MNKQQQTALNMARFIRSQSLILLEKLDALDADEQAAMCERLHEL AEELQNSIQARFEAESETGT" gene 3486..3857 /locus_tag="YP_pPCP04" /db_xref="GeneID:2767720" CDS 3486..3857 /locus_tag="YP_pPCP04" /note="Best Blastp hit = gi|321919|pir||JQ1541 hypothetical 16.9K protein - Salmonella typhi murium plasmid NTP16." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="NP_995570.1" /db_xref="GI:45478715" /db_xref="GeneID:2767720" /translation="MSKKRRPQKRPRRRRFFHRLRPPDEHHKNRRSSQRWRNPTGLKD TRRFPPEAPSCALLFRPCRLPDTSPPFSLREAWRFLIAHAVGISVRCRSFAPSWAVCT NPPFSPTTAPYPVTIVLSPTR" misc_feature 3498..3626 /locus_tag="YP_pPCP04" /note="ProfileScan match to entry PS50323 ARG_RICH, E-value 8.981" gene 4343..4780 /gene="pim" /locus_tag="YP_pPCP05" /db_xref="GeneID:2767712" CDS 4343..4780 /gene="pim" /locus_tag="YP_pPCP05" /note="similar to many previously sequenced pesticin immunity protein entries of Yersinia pestis plasmid pPCP, e.g. gi| 16082683|,ref|NP_395230.1| (NC_003132) , gi|1200166|emb|CAA90861.1| (Z54145 ) , gi|1488655| emb|CAA63439.1| (X92856) , gi|2996219|gb|AAC62543.1| (AF053945) , and gi|5763814|emb|CAB531 67.1| (AL109969)" /codon_start=1 /transl_table=11 /product="pesticin immunity protein" /protein_id="NP_995571.1" /db_xref="GI:45478716" /db_xref="GeneID:2767712" /translation="MGGGMISKLFCLALIFLSSSGLAEKNTYTAKDILQNLELNTFGN SLSHGIYGKQTTFKQTEFTNIKSNTKKHIALINKDNSWMISLKILGIKRDEYTVCFED FSLIRPPTYVAIHPLLIKKVKSGNFIVVKEIKKSIPGCTVYYH" gene complement(4815..5888) /gene="pst" /locus_tag="YP_pPCP06" /db_xref="GeneID:2767721" CDS complement(4815..5888) /gene="pst" /locus_tag="YP_pPCP06" /note="Best Blastp hit =|16082684|ref|NP_395231.1| (NC_003132) pesticin [Yersinia pestis], gi|984824|gb|AAA75369.1| (U31974) pesticin [Yersinia pestis], gi|1488654|emb|CAA63438.1| (X92856) pesticin [Yersinia pestis], gi|2996220|gb|AAC62544.1| (AF053945) pesticin [Yersinia pestis], gi|5763815|emb|CAB53168.1| (AL1099 69) pesticin [Yersinia pestis]" /codon_start=1 /transl_table=11 /product="pesticin" /protein_id="NP_995572.1" /db_xref="GI:45478717" /db_xref="GeneID:2767721" /translation="MSDTMVVNGSGGVPAFLFSGSTLSSYRPNFEANSITIALPHYVD LPGRSNFKLMYIMGFPIDTEMEKDSEYSNKIRQESKISKTEGTVSYEQKITVETGQEK DGVKVYRVMVLEGTIAESIEHLDKKENEDILNNNRNRIVLADNTVINFDNISQLKEFL RRSVNIVDHDIFSSNGFEGFNPTSHFPSNPSSDYFNSTGVTFGSGVDLGQRSKQDLLN DGVPQYIADRLDGYYMLRGKEAYDKVRTAPLTLSDNEAHLLSNIYIDKFSHKIEGLFN DANIGLRFSDLPLRTRTALVSIGYQKGFKLSRTAPTVWNKVIAKDWNGLVNAFNNIVD GMSDRRKREGALVQKDIDSGLLK" variation 5910..5911 /note="compared to AF053945" /replace="" variation 5933^5934 /note="compared to AL109969" /replace="a" variation 5933^5934 /note="compared to AF053945" /replace="aa" variation 5948 /note="compared to AL109969" /replace="c" gene 6005..6421 /locus_tag="YP_pPCP07" /db_xref="GeneID:2767719" CDS 6005..6421 /locus_tag="YP_pPCP07" /note="Best Blastp hit = gi|16082685|ref|NP_395232.1| (NC_003132) hypothetical protein [Yersinia pestis], gi|5763816|emb|CAB53169.1| (AL109969) hypothetical protein [Yersinia pestis]" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="NP_995573.1" /db_xref="GI:45478718" /db_xref="GeneID:2767719" /translation="MKFHFCDLNHSYKNQEGKIRSRKTAPGNIRKKQKGDNVSKTKSG RHRLSKTDKRLLAALVVAGYEERTARDLIQKHVYTLTQADLRHLVSEISNGVGQSQAY DAIYQARRIRLARKYLSGKKPEGVEPREGQEREDLP" variation 6525 /note="compared to AF053945 and AL109969" /replace="c" gene 6664..7602 /gene="pla" /locus_tag="YP_pPCP08" /db_xref="GeneID:2767715" CDS 6664..7602 /gene="pla" /locus_tag="YP_pPCP08" /EC_number="3.4.23.48" /note="outer membrane protease; involved in virulence in many organisms; OmpT; IcsP; SopA; Pla; PgtE; omptin; in Escherichia coli OmpT can degrade antimicrobial peptides; in Yersinia Pla activates plasminogen during infection; in Shigella flexneria SopA cleaves the autotransporter IcsA" /codon_start=1 /transl_table=11 /product="outer membrane protease" /protein_id="NP_995574.1" /db_xref="GI:45478719" /db_xref="GeneID:2767715" /translation="MKKSSIVATIITILSGSANAASSQLIPNISPDSFTVAASTGMLS GKSHEMLYDAETGRKISQLDWKIKNVAILKGDISWDPYSFLTLNARGWTSLASGSGNM DDYDWMNENQSEWTDHSSHPATNVNHANEYDLNVKGWLLQDENYKAGITAGYQETRFS WTATGGSYSYNNGAYTGNFPKGVRVIGYNQRFSMPYIGLAGQYRINDFELNALFKFSD WVRAHDNDEHYMRDLTFREKTSGSRYYGTVINAGYYVTPNAKVFAEFTYSKYDEGKGG TQIIDKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF" gene complement(7789..8088) /locus_tag="YP_pPCP09" /db_xref="GeneID:2767713" CDS complement(7789..8088) /locus_tag="YP_pPCP09" /note="Best Blastp hit = gi|16082687|ref|NP_395234.1| (NC_003132) putative transcriptional regulator [Yersinia pestis], gi|5763818|emb|CAB53171.1| (AL109969) putative transcriptional regulator [Yersinia pestis]." /codon_start=1 /transl_table=11 /product="putative transcriptional regulator" /protein_id="NP_995575.1" /db_xref="GI:45478720" /db_xref="GeneID:2767713" /translation="MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQK QVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMGGKLSLDVELPTGRRVAFHV" gene complement(8088..8360) /locus_tag="YP_pPCP10" /db_xref="GeneID:2767714" CDS complement(8088..8360) /locus_tag="YP_pPCP10" /note="Best Blastp hit = gi|16082688|ref|NP_395235.1| (NC_003132) hypothetical protein [ Yersinia pestis], gi|5763819|emb|CAB53172.1| (AL109969) hypothetical protein [Yersinia pestis]" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="NP_995576.1" /db_xref="GI:45478721" /db_xref="GeneID:2767714" /translation="MADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRA FYAFDPIRRAIVLCAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK" variation 8529^8530 /note="compared to AL109969" /replace="tt" ORIGIN 1 tgtaacgaac ggtgcaatag tgatccacac ccaacgcctg aaatcagatc cagggggtaa 61 tctgctctcc tgattcagga gagtttatgg tcacttttga gacagttatg gaaattaaaa 121 tcctgcacaa gcagggaatg agtagccggg cgattgccag agaactgggg atctcccgca 181 ataccgttaa acgttatttg caggcaaaat ctgagccgcc aaaatatacg ccgcgacctg 241 ctgttgcttc actcctggat gaataccggg attatattcg tcaacgcatc gccgatgctc 301 atccttacaa aatcccggca acggtaatcg ctcgcgagat cagagaccag ggatatcgtg 361 gcggaatgac cattctcagg gcattcattc gttctctctc ggttcctcag gagcaggagc 421 ctgccgttcg gttcgaaact gaacccggac gacagatgca ggttgactgg ggcactatgc 481 gtaatggtcg ctcaccgctt cacgtgttcg ttgctgttct cggatacagc cgaatgctgt 541 acatcgaatt cactgacaat atgcgttatg acacgctgga gacctgccat cgtaatgcgt 601 tccgcttctt tggtggtgtg ccgcgcgaag tgttgtatga caatatgaaa actgtggttc 661 tgcaacgtga cgcatatcag accggtcagc accggttcca tccttcgctg tggcagttcg 721 gcaaggagat gggcttctct ccccgactgt gtcgcccctt cagggcacag actaaaggta 781 aggtggaacg gatggtgcag tacacccgta acagttttta catcccacta atgactcgcc 841 tgcgcccgat ggggatcact gtcgatgttg aaacagccaa ccgccacggt ctgcgctggc 901 tgcacgatgt cgctaaccaa cgaaagcatg aaacaatcca ggcccgtccc tgcgatcgct 961 ggctcgaaga gcagcagtcc atgctggcac tgcctccgga gaaaaaagag tatgacgtgc 1021 atcttgatga aaatctggtg aacttcgaca aacaccccct gcatcatcca ctctccatct 1081 acgactcatt ctgcagagga gtggcgtgat gatggaactg caacatcaac gactgatggc 1141 gctcgccggg cagttgcaac tggaaagcct tataagcgca gcgcctgcgc tgtcacaaca 1201 ggcagtagac caggaatgga gttatatgga cttcctggag catctgcttc atgaagaaaa 1261 actggcacgt catcaacgta aacaggcgat gtatacccga atggcagcct tcccggcggt 1321 gaaaacgttc gaagagtatg acttcacatt cgccaccgga gcaccgcaga agcaactcca 1381 gtcgttacgc tcactcagct tcatagaacg taatgaaaat atcgtattac tggggccatc 1441 aggtgtgggg aaaacccatc tggcaatagc gatgggctat gaagcagtcc gtgcaggtat 1501 caaagttcgc ttcacaacag cagcagatct gttacttcag ttatctacgg cacaacgtca 1561 gggccgttat aaaacgacgc ttcagcgtgg agtaatggcc ccccgcctgc tcatcattga 1621 tgaaataggc tatctgccgt tcagtcagga agaagcaaag ctgttcttcc aggtcatcgc 1681 taaacgttac gaaaagagcg caatgatcct gacatccaat ctgccgttcg ggcagtggga 1741 tcaaacgttc gccggtgatg cagcactgac ctcagcgatg ctggaccgta tcttacacca 1801 ctcacatgtc gttcaaatca aaggagaaag ctatcgactc agacagaaac gaaaggccgg 1861 ggttatagca gaagctaatc ctgagtaaaa cggtggatca atattgggcc gttggtggag 1921 atataagtgg atcacttttc atccgtcgtt gacaccctga tgaattcacg tgttcacgcc 1981 tgaataacaa gaatgccgga gatacgcagt catatttttt acacaattct ctaatcccga 2041 caaggtcgta ggtcgttata ggaaaattct tagcaccatt ccggaacaat cagaacagca 2101 ggccatgaac gactgacaac attacgaata taaaaaacgc acccgggcca gacattcccc 2161 ctactgatta aaccagccgg acttgtccac ggaacggtct ttttaaaccg acacacagtc 2221 tgagtacaga tacatgtcac gatgatgcag gattagcgga agagtgtgag cacgtttccg 2281 ggaactgtgg tgaaccatag ctcaatattc gagtgagggc ataccggaaa cgcgctcaga 2341 ttcgttgtaa cgcgattttc cgtaccgggc aattttttca gttgtttttt cgtttcatgt 2401 cgtcagaaac gttctgagcg cgtttccggc atctgatgct acgcaaacca tccccatggt 2461 cagttgacag ccggaaacac gcgggtgtcg ttttagcgta tcgacgggac ggcgtcgaga 2521 agcacaaaaa acagatgttg tactcagtca gttgttttac agacagcact gcggcagatt 2581 gaaaaagtac cgtactttca ggaatgtcca gaaaccatgt gtcagacttc gttctccccc 2641 ttccgggtga atttttttgt catccgttca ggaatctctt tataacgatt actccatttc 2701 aggatttttt atgtggcgtt tactacaggc aggatattca aaggcaaaaa aatcccccgg 2761 aacaggcgga acccggacag ggggagaacg aatcgctaaa taattttcgt agttgtattt 2821 cccatcgttg ctactgcaac gggatgaatt tgccgcagtt tatcctgtaa aacaatcctg 2881 atttactcac actccacata tcactgacgg agcacaacgg aatagtgaac aaacaacaac 2941 aaactgcgct gaatatggcg cgatttatca gaagccagag cctgatactg cttgaaaaac 3001 tggatgctct ggatgccgac gagcaggcgg ccatgtgtga acgactgcac gaactcgcgg 3061 aagaactcca gaacagcatc caggctcgct ttgaagccga aagtgaaaca ggaacataac 3121 gaagctcccg gagacggtca cagcttgtct gtgaacggat gccgggagca gacaagcccg 3181 tcagggcgcg tcagcgggtt ttagcgggtg tcggggcgca gccatgaccc agtcacgtag 3241 cgatagcgga gtgtatactg gcttagtcat gcggcatcag tgcggattgt atgaaaagtg 3301 caccatgtac ggtgtgaaat gccgcacaga tgcgtaagga gaacatgcag atgccgatgc 3361 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggtg 3421 tctgctcact caaaagcggt gatactgtta tccacacaat caggggataa cgccggaaag 3481 aacatgtgag caaaaaacga agaccccaga aaaggccgcg ccggaggcgc tttttccata 3541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 3601 cgacaggact taaagatacc aggcgtttcc ccccggaagc tccctcgtgc gctctcctgt 3661 tccgaccctg ccgcttaccg gatacctctc cgcctttctc ccttcgggaa gcgtggcgct 3721 ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 3781 ctgtgtgcac gaaccccccg ttcagcccga ccactgcgcc ttatccggta actatcgtct 3841 tgagtccaac ccggtaagac acgactttac gccactggca gcagccattg gtaactgaaa 3901 agtggattta gatacgcaga actcttgaag ttgaagcctt atcgcggcta cactgaaagg 3961 acagcatttg gtatctgtgc tccacttaag ccagctacca caggttagaa agcctgagaa 4021 acttctaacc ttcgaaagaa cccacgcctg agaacgtggg ttttttcgtt tacaggcagc 4081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaattgc 4141 gctcccgatc agttcagcag aagattatga tggggttcta tgggtattgc tgcggtaaca 4201 cccatgttac ttgaggttgt atgtagtctg tgtagaatta tacacataag gcttaaactg 4261 ctcttttttt tcaatatgca attggaagtt cattgactac ataaatagat tattccaaat 4321 aatttattta tgtaagaaca ggatgggagg gggaatgatc tcaaagttat tttgcttggc 4381 tctcatattt ttatcatcaa gtggccttgc agaaaaaaac acatatacag caaaagacat 4441 cttgcaaaac ctagaattaa atacctttgg caattcattg tctcatggca tctatgggaa 4501 acagacaacc ttcaagcaaa ccgagtttac aaatattaaa agcaacacca aaaaacacat 4561 tgcacttatc aataaagaca actcatggat gatatcatta aaaatactag gaattaagag 4621 agatgagtat actgtctgtt ttgaagattt ctctctaata agaccgccaa catatgtagc 4681 catacatcct ctacttataa aaaaagtaaa atctggaaac tttatagtag tgaaagaaat 4741 aaagaaatct atccctggtt gcactgtata ttatcattaa tagcaagccc ctcattatta 4801 tgaggggctc atggttattt taacaatcca ctatcgatat ctttttgcac cagagcgccc 4861 tctcgtttac gtctgtcaga cattccatca acaatattat taaaagcatt tacaaggcca 4921 ttccagtctt ttgcgataac tttattccat actgtgggag cagttctgga taacttaaac 4981 cctttttgat atccaataga caccagtgct gtacgggttc tcaacggtaa atcgctgaac 5041 cgaagaccga tattagcgtc attgaaaaga ccttcaatct tatgtgagaa tttatcaata 5101 taaatattag ataagagatg agcttcatta tcagaaagcg tcagaggtgc tgttctcact 5161 ttatcataag cctccttccc tcgaagcata taatacccat caagtctatc tgcaatatac 5221 tgagggacac cgtcattcaa taaatcctgt ttgcttcgct gaccaaggtc aaccccggaa 5281 ccgaatgtaa caccggtact gttaaaataa tcgctactag gattagacgg aaaatgactt 5341 gtcggattaa acccttcaaa accattactg gagaaaatat cgtggtcaac aatatttacc 5401 gaacgacgta aaaattcctt cagttgacta atattgtcaa agttaatgac agtgttgtcc 5461 gctaggacga tgcgatttcg gttattattc agaatgtctt cgttctcttt cttatcgaga 5521 tgttcaatag attcggcaat cgttccctca agaaccatga cacggtagac tttcacaccg 5581 tctttttcct gacctgtttc aacagttatt ttctgttcgt aagacacggt cccttcagtt 5641 tttgaaattt tactttcctg gcggatctta tttgaatatt cactgtcttt ctccatctcc 5701 gtatcaatcg gaaaccccat aatgtacatc agtttaaaat tactccggcc aggcagatcc 5761 acataatgtg gtaatgcaat tgtaatcgaa ttagcttcaa aatttggtct gtaactgctt 5821 aatgtacttc cggaaaagag aaaagccgga acaccacctg aaccattcac taccattgta 5881 tctgacataa aaattcctct ttaacacata aaaaaaacaa taagttaaaa aaatactgta 5941 cataaaagca ctgtttttat gtacagtaat aaaattacgc cgctttattt tctctgtcaa 6001 taatatgaaa tttcattttt gtgatctgaa tcactcttat aaaaatcagg aagggaagat 6061 tcgcagcaga aaaacagcac cgggtaacat cagaaaaaaa cagaaaggag ataacgtgag 6121 caaaacaaaa tctggtcgcc accgactgag caaaacagac aaacgcctgc tggctgcact 6181 tgtcgttgcc ggatacgaag aacggacagc ccgtgacctc atccagaaac acgtttacac 6241 actgacacag gccgacctgc gccatctggt cagtgaaatc agtaacggtg tgggacagtc 6301 acaggcctac gatgcgattt accaggcgag acgcattcgt ctcgcccgta aatacctgag 6361 cggaaaaaaa ccggaagggg tggaaccccg ggaagggcag gaacgggaag atttaccata 6421 actcccgtta tcagtaccat cggctcaacg ctcgttgtcg gatctgaaaa attcgctcaa 6481 aagatcatat ttccctggat attttccacc gtttcttatg tgagaaaagt cacataattc 6541 tgtcagacga cgagaaaacg gatatcgatt attgtttaat atttttacat tattaaaaat 6601 gaaattagat aatcagatac aaataatatg ttttcgttca tgcagagaga ttaagggtgt 6661 ctaatgaaga aaagttctat tgtggcaacc attataacta ttctgtccgg gagtgctaat 6721 gcagcatcat ctcagttaat accaaatata tcccctgaca gctttacagt tgcagcctcc 6781 accgggatgc tgagtggaaa gtctcatgaa atgctttatg acgcagaaac aggaagaaag 6841 atcagccagt tagactggaa gatcaaaaat gtcgctatcc tgaaaggtga tatatcctgg 6901 gatccatact catttctgac cctgaatgcc agggggtgga cgtctctggc ttccgggtca 6961 ggtaatatgg atgactacga ctggatgaat gaaaatcaat ctgagtggac agatcactca 7021 tctcatcctg ctacaaatgt taatcatgcc aatgaatatg acctcaatgt gaaaggctgg 7081 ttactccagg atgagaatta taaagcaggt ataacagcag gatatcagga aacacgtttc 7141 agttggacag ctacaggtgg ttcatatagt tataataatg gagcttatac cggaaacttc 7201 ccgaaaggag tgcgggtaat aggttataac cagcgctttt ctatgccata tattggactt 7261 gcaggccagt atcgcattaa tgattttgag ttaaatgcat tatttaaatt cagcgactgg 7321 gttcgggcac atgataatga tgagcactat atgagagatc ttactttccg tgagaagaca 7381 tccggctcac gttattatgg taccgtaatt aacgctggat attatgtcac acctaatgcc 7441 aaagtctttg cggaatttac atacagtaaa tatgatgagg gcaaaggagg tactcagatc 7501 attgataaga atagtggaga ttctgtctct attggcggag atgctgccgg tatttccaat 7561 aaaaattata ctgtgacggc gggtctgcaa tatcgcttct gaaaaataca gatcatatct 7621 ctcttttcat cctcccctag cggggaggat gtctgtggaa aggaggttgg tgtttgacca 7681 accttcagat gtgtgaaaaa tcaccttttt caccataatg acggggcgct cattctgttg 7741 ttttgccttg acattctcca cgtctttcag ggcatggaga aggtcaaatt agacatggaa 7801 cgctactctc cttcctgtag gaagctcaac atccaagctt aatttgcctc ccattgcttc 7861 aacgtaacgc tttaacgtcg ccagctttaa atcatttccg cgctgctcca gctttgttac 7921 tgctggctgg cttataccca tcgcctcagc aacttgtttt tgtgataact ggagttcttc 7981 acgcatcatc tgcaagccga cctcaagaat catctcatct gccatttctt taattcgtgt 8041 ctggctttca ggtgaacgac tggcaatcac ctcatctaat gttctcatta cttgctctcc 8101 agtgtgttca gatgtgctgt aaattcatcc tcagctatac gcaccagttt ttcataaaac 8161 cgcttatcat tacttttatc tcctgcacaa agaacgatag cccgacgaat cggatcgaac 8221 gcataaaagg ctcttatcgg acggccagaa aactgaacgc gaagctcttt catatttttg 8281 taccgagaac ctttcacggt atcggcatat ggcctgggta actcaggtcc gtaaacctgt 8341 agctttttca aatcagccaa aaccttttcc tgaagagcgt cttcttgctc atttagccag 8401 tcgtcaaatc gctggctaaa aagtaccatc cacatgctca accctataac ctgtagctta 8461 ccccactaac aatataacct acgagttata ttttcaagaa aagctggcta tttaacataa 8521 cggcaatttg tacgcaccac tgaaatgcgt tcagcgcgat cacggcaaca gacaggcaaa 8581 aatagcaaca aacctcccga aaaaccgccg cgatcgcgcc tgataaattt taaccttatg 8641 catatctatg cagccaggcg aatcacgaac gaattgcctg cctgatgtaa ctgaaacggg 8701 tgttttttcc tgatttggtg ggcgtggaag acggaacatg aacgggaaaa cagaattcat 8761 gccagatgag cgcgatctgg caattaaggc aaaacacagc aacaaagaca cgccagaatc 8821 gcgcccggat atgttttaac gcgattttca gactcagaca aattcagcag aatgctactc 8881 cattcaccgg gctgatggtg aatacatgcg tatccaggat gagtacattt ctggctctgc 8941 cacagctctg tctgttggca gctttcgcct gtccggaaac ctgcttaaaa cgctcccgaa 9001 aggcctctga accagaaagc aacaaaacac aggccattaa gtaaatcgcg ttaaaacacg 9061 tctgatggat tgctgcaaaa aaaagtccct aatggagcag ggactgttaa acccagtgaa 9121 tagcgtctaa attaaagtaa gaatacgacc aggtactctt cagaaaagag attaatccac 9181 cgcacagaat aatcaacagt aaaaacaaac aaccctgatt ttttattttt ctttttttcg 9241 ataaaaacaa aattaaagaa ataattaatc agaacattcc ttaacttcag ggcattgcct 9301 gtgttccatt ttgtgattag tctgaaactt ccgaaggtgg ataacacccg gtattttttt 9361 gctcacataa agcccctcct tcaggcagag gggctttttc tttgccacca cataaaaaag 9421 gccctcacag gaggtgttct gtgagggcgt atgataagga ctgaatcgat ggttaatatg 9481 tctagtcctg acttttgcat ctccgaatat aaaaccctgt ttaacggcat gcaaaaccaa 9541 aaaataaaaa tgtgacatcg caatgccaga taatattgac gcatgaggga atgcgtaccc 9601 cgacccctg //