ID   EDD_RAT        STANDARD;      PRT;   920 AA.
AC   Q62671;
DT   01-NOV-1997 (Rel. 35, Created)
DT   15-JUN-2002 (Rel. 41, Last sequence update)
DT   15-JUN-2002 (Rel. 41, Last annotation update)
DE   Ubiquitin--protein ligase EDD (EC 6.3.2.-) (Hyperplastic discs
DE   protein homolog) (100 kDa protein) (Fragment).
GN   EDD OR HYD.
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Rattus.
RN   [1]
RP   SEQUENCE FROM N.A.
RC   STRAIN=Wistar; TISSUE=Testis;
RA   Mueller D., Rehbein M., Baumeister H., Richter D.;
RT   "Molecular characterization of a novel rat protein structurally
RT   related to poly(A) binding proteins and the 70K protein of the U1
RT   small nuclear ribonucleoprotein particle (snRNP).";
RL   Nucleic Acids Res. 20:1471-1475(1992).
RN   [2]
RP   ERRATUM.
RA   Mueller D., Rehbein M., Baumeister H., Richter D.;
RL   Nucleic Acids Res. 20:2624-2624(1992).
RN   [3]
RP   IDENTIFICATION OF PROBABLE FRAMESHIFT.
RA   Callaghan M.J., Russell A.J., Woollatt E., Sutherland G.R.,
RA   Sutherland R.L., Watts C.K.W.;
RT   "Identification of a human HECT family protein with homology to the
RT   Drosophila tumor suppressor gene hyperplastic discs.";
RL   Oncogene 17:3479-3491(1998).
CC   -!- FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from
CC       an E2 ubiquitin-conjugating enzyme in the form of a thioester and
CC       then directly transfers the ubiquitin to targeted substrates (By
CC       similarity). May be involved in maturation and/or post-
CC       transcriptional regulation of mRNA. May play a role in control of
CC       cell cycle progression. May have tumor suppressor function.
CC       Regulates DNA topoisomerase II binding protein (TopBP1) for the
CC       DNA damage response (By similarity).
CC   -!- SUBCELLULAR LOCATION: Nuclear (By similarity).
CC   -!- TISSUE SPECIFICITY: Highest levels found in testis. Also present
CC       in liver, kidney, lung and brain.
CC   -!- DEVELOPMENTAL STAGE: In early post-natal life, expression in
CC       the testis increases to reach a maximum around day 28.
CC   -!- MISCELLANEOUS: A cysteine residue is required for ubiquitin-
CC       thiolester formation.
CC   -!- SIMILARITY: CONTAINS 1 HECT-TYPE E3 UBIQUITIN-PROTEIN LIGASE
CC       DOMAIN.
CC   -!- CAUTION: Ref.1 sequence differs from that shown due to a
CC       frameshift in position 30.
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DR   EMBL; X64411; CAA45756.1; ALT_FRAME.
DR   HSSP; O95071; 1I2T.
DR   InterPro; IPR000569; HECT_domain.
DR   InterPro; IPR002004; PABP/HECT.
DR   Pfam; PF00632; HECT; 1.
DR   Pfam; PF00658; PABP; 1.
DR   SMART; SM00119; HECTc; 1.
DR   SMART; SM00517; PolyA; 1.
DR   PROSITE; PS50237; HECT; 1.
KW   Ubl conjugation pathway; Ligase; Nuclear protein.
FT   NON_TER       1      1
FT   DOMAIN      515    571       PABP-LIKE.
FT   DOMAIN      583    920       HECT.
FT   DOMAIN      108    119       ASP/GLU-RICH (ACIDIC).
FT   DOMAIN      158    181       PRO-RICH.
FT   DOMAIN      451    470       ARG/GLU-RICH (MIXED CHARGE).
FT   DOMAIN      479    488       ARG/ASP-RICH (MIXED CHARGE).
FT   DOMAIN      610    621       ASP/GLU-RICH (ACIDIC).
FT   DOMAIN      858    878       PRO-RICH.
FT   BINDING     889    889       UBIQUITIN (BY SIMILARITY).
SQ   SEQUENCE   920 AA;  103949 MW;  465771084536C3AA CRC32;
     ARRERMTARE EASLRTLEGR RRATLLSARQ GMMSARGDFL NYALSLMRSH NDEHSDVLPV
     LDVCSLKHVA YVFQALIYWI KAMNQQTTLD TPQLERKRTR ELLELGIDNE DSEHENDDDT
     SQSATLNDKD DESLPAETGQ NHPFFRRSDS MTFLGCIPPN PFEVPLAEAI PLADQPHLLQ
     PNARKEDLFG RPSQGLYSSS AGSGKCLVEV TMDRNCLEVL PTKMSYAANL KNVMNMQNRQ
     KKAGEDQSML AEEADSSKPG PSAHDVAAQL KSSLLAEIGL TESEGPPLTS FRPQCSFMGM
     VISHDMLLGR WRLSLELFGR VFMEDVGAEP GSILTELGGF EVKESKFRRE MEKLRNQQSR
     DLSLEVDRDR DLLIQQTMRQ LNNHFGRRCA TTPMAVHRVK VTFKDEPGEG SGVARSFYTA
     IAQAFLSNEK LPNLDCIQNA NKGTHTSLMQ RLRNRGERDR EREREREMRR SSGLRAGSRR
     DRDRDFRRQL SIDTRPFRPA SEGNPSDDPD PLPAHRQALG ERLYPRVQAM QPAFASKITG
     MLLELSPAQL LLLLASEDSL RARVEEAMEL IVAHGRENGA DSILDLGLLD SSEKVQENRK
     RHGSSRSVVD MDLDDTDDGD DNAPLFYQPG KRGFYTPRPG KNTEARLNCF RNIGRILGLC
     LLQNELCPIT LNRHVIKVLL GRKVNWHDFA FFDPVMYESL RQLILASQSS DADAVFSAMD
     LAFAVDLCKE EGGGQVELIP NGVNIPVTPQ NVYEYVRKYA EHRMLVVAEQ PLHAMRKGLL
     DVLPKNSLED LTAEDFRLLV NGCGEVNVQM LISFTSFNDE SGENAEKLLQ FKRWFWSIVE
     RMSMTERQDL VYFWTSSPSL PASEEGFQPM PSITIRPPDD QHLPTANTCI SRLYVPLYSS
     KQILKQKLLL AIKTKNFGFV
//