Sample Summary Information For Experiment (FC626BWAAXX)
Project Summary
| Project Name | FC626BWAAXX |
| Machine | MONDAS33 |
| Run Folder | 100723_EAS346_0188_FC626BWAAXX |
| Flow Cell ID | FC626BWAAXX |
| Platform | |
| Control Software | SCS |
| Primary Analysis | RTA 1.9.10.0 |
| Secondary Analysis | CASAVA-1.8.2 |
Project Results Summary
| Clusters (Raw) | Clusters(PF) | Yield (MBases) |
|---|---|---|
| 504,044 | 504,044 | 23 |
Barcode-Lane summary
| Barcode-Lane | Sample | Barcode | Lane | Species | Analysis Type | Length | Num Tiles | Genome Directory |
|---|
| lane4_NoIndex_L4 | lane4 | NoIndex | 4 | Unknown | eland pair | 45 45 | 2 | /illumina/development/casava/Version_1_8_branch/share/CASAVA-1.8.2/examples/Validation/100723_EAS346_0188_FC626BWAAXX/Data/Intensities/BaseCalls/controls.fasta |
| lane8_NoIndex_L8 | lane8 | NoIndex | 8 | Unknown | eland extended | 45 | 2 | /illumina/development/casava/Version_1_8_branch/share/CASAVA-1.8.2/examples/Validation/100723_EAS346_0188_FC626BWAAXX/Data/Intensities/BaseCalls/controls.fasta |
Sample Results Summary : Read 1
| Sample Info | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | Sample Yield (Mbases) | Clusters (raw) | Clusters (PF) | 1st Cycle Int (PF) | % intensity after 20 cycles (PF) | % PF Clusters | % Align (PF) | Alignment Score (PF) | % Mismatch Rate (PF) | % >=Q30 bases (PF) | Mean Quality SCore (PF) |
| lane4 | 0 | 2,765 | 2,765 | 221 | 97.52 | 100.00 | 13.96 | 28.12 | 1.44 | 96.46 | 38.36 |
| lane8 | 23 | 501,279 | 501,279 | 228 | 94.34 | 100.00 | 0.01 | 0.01 | 10.25 | 96.85 | 38.41 |
Sample Results Summary : Read 2
| Sample Info | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | Sample Yield (Mbases) | Clusters (raw) | Clusters (PF) | 1st Cycle Int (PF) | % intensity after 20 cycles (PF) | % PF Clusters | % Align (PF) | Alignment Score (PF) | % Mismatch Rate (PF) | % >=Q30 bases (PF) | Mean Quality SCore (PF) |
| lane4 | 0 | 2,765 | 2,765 | 193 | 80.82 | 100.00 | 51.72 | 114.82 | 0.15 | 92.41 | 36.81 |
| lane8 | 0 | 0 | 0 | 190 | 86.47 |
Expanded Sample Summary : Read 1
| Sample Info | Phasing Info | Raw data | Filtered data | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | Clusters (raw) | % Phasing | % Prephasing | % Mismatch Rate (raw) | % PF Clusters | Cycle 2-4 Av Int (PF) | Cycle 2-10 Av % Loss (PF) | Cycle 10-20 Av % Loss (PF) | % Align (PF) | % Mismatch Rate (PF) | % >=Q30 bases (PF) | Mean Quality SCore (PF) |
| lane4 | 2,765 | 0.19 | 0.44 | 1.44 | 100.00 | 233.26 | 1.34 | 0.95 | 13.96 | 1.44 | 96.46 | 38.36 |
| lane8 | 501,279 | 0.19 | 0.44 | 10.25 | 100.00 | 231.81 | 1.47 | 0.77 | 0.01 | 10.25 | 96.85 | 38.41 |
Expanded Sample Summary : Read 2
| Sample Info | Phasing Info | Raw data | Filtered data | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | Clusters (raw) | % Phasing | % Prephasing | % Mismatch Rate (raw) | % PF Clusters | Cycle 2-4 Av Int (PF) | Cycle 2-10 Av % Loss (PF) | Cycle 10-20 Av % Loss (PF) | % Align (PF) | % Mismatch Rate (PF) | % >=Q30 bases (PF) | Mean Quality SCore (PF) |
| lane4 | 2,765 | 0.80 | 0.66 | 0.15 | 100.00 | 187.99 | 1.34 | 0.95 | 51.72 | 0.15 | 92.41 | 36.81 |
| lane8 | 0 | 0.80 | 0.66 | 186.55 | 1.47 | 0.77 |
IVC Plots
click hereAll Intensity Plots
click hereMismatch Graphs
click hereMismatch Curves
click hereAdditional Paired Statistics
| Sample Info | Relative Orientation Statistics | Insert Size Statistics (for relative orientation R+) | Insert Statistics (% of individually uniquely alignable pairs) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | F-: >R2 R1> |
F+: >R1 R2> |
R-: <R2 R1> |
R+: >R1 R2< |
Total | Median | Below-median SD | Above-median SD | Low thresh. | High thresh. | Too small | Too large | Orientation and size OK |
| lane4 | 2 (0.33%) |
3 (0.49%) |
21 (3.42%) |
588 (95.77%) |
614 | 309 | 100 | 29 | 57 | 377 | 0 (0.00%) |
1 (0.16%) |
587 (95.60%) |
| lane8 | 0 |
CASAVA-1.8.2