Barcode-Lane Summary Information For Experiment (FC626BWAAXX)

Project Summary

Project Name FC626BWAAXX
Machine MONDAS33
Run Folder 100723_EAS346_0188_FC626BWAAXX
Flow Cell ID FC626BWAAXX
Platform
Control Software SCS
Primary Analysis RTA 1.9.10.0
Secondary Analysis CASAVA-1.8.2

Project Results Summary

Clusters (Raw) Clusters(PF) Yield (MBases)
504,044 504,044 23

Barcode-Lane summary

Barcode-Lane Sample Barcode Lane Species Analysis Type Length Num Tiles Genome Directory
lane4_NoIndex_L4 lane4 NoIndex 4 Unknown eland pair 45 45 2 /illumina/development/casava/Version_1_8_branch/share/CASAVA-1.8.2/examples/Validation/100723_EAS346_0188_FC626BWAAXX/Data/Intensities/BaseCalls/controls.fasta
lane8_NoIndex_L8 lane8 NoIndex 8 Unknown eland extended 45 2 /illumina/development/casava/Version_1_8_branch/share/CASAVA-1.8.2/examples/Validation/100723_EAS346_0188_FC626BWAAXX/Data/Intensities/BaseCalls/controls.fasta

Barcode-Lane Results Summary : Read 1

Barcode-Lane Info
Barcode lane Barcode lane Yield (Mbases) Clusters (raw) Clusters (PF) 1st Cycle Int (PF) % intensity after 20 cycles (PF) % PF Clusters % Align (PF) Alignment Score (PF) % Mismatch Rate (PF) % >=Q30 bases (PF) Mean Quality SCore (PF)
lane4_NoIndex_L4 0 2,765 2,765 221 +/-16 97.52 +/-0.67 100.00 13.96 28.12 1.44 96.46 38.36
lane8_NoIndex_L8 23 501,279 501,279 228 +/-35 94.34 +/-3.54 100.00 0.01 0.01 10.25 96.85 38.41

Barcode-Lane Results Summary : Read 2

Barcode-Lane Info
Barcode lane Barcode lane Yield (Mbases) Clusters (raw) Clusters (PF) 1st Cycle Int (PF) % intensity after 20 cycles (PF) % PF Clusters % Align (PF) Alignment Score (PF) % Mismatch Rate (PF) % >=Q30 bases (PF) Mean Quality SCore (PF)
lane4_NoIndex_L4 0 2,765 2,765 193 +/-7 80.82 +/-2.14 100.00 51.72 114.82 0.15 92.41 36.81
lane8_NoIndex_L8 0 0 0 190 +/-20 86.47 +/-0.18

Expanded Barcode-Lane Summary : Read 1

Barcode lane Info Phasing Info Raw data Filtered data
Barcode lane Clusters (raw) % Phasing % Prephasing % Mismatch Rate (raw) % PF Clusters Cycle 2-4 Av Int (PF) Cycle 2-10 Av % Loss (PF) Cycle 10-20 Av % Loss (PF) % Align (PF) % Mismatch Rate (PF) % >=Q30 bases (PF) Mean Quality SCore (PF)
lane4_NoIndex_L4 2,765 0.19 0.44 1.44 100.00 233.26 +/-7.03 1.34 +/-0.05 0.95 +/-0.03 13.96 1.44 96.46 38.36
lane8_NoIndex_L8 501,279 0.19 0.44 10.25 100.00 231.81 +/-30.87 1.47 +/-0.24 0.77 +/-0.01 0.01 10.25 96.85 38.41

Expanded Barcode-Lane Summary : Read 2

Barcode lane Info Phasing Info Raw data Filtered data
Barcode lane Clusters (raw) % Phasing % Prephasing % Mismatch Rate (raw) % PF Clusters Cycle 2-4 Av Int (PF) Cycle 2-10 Av % Loss (PF) Cycle 10-20 Av % Loss (PF) % Align (PF) % Mismatch Rate (PF) % >=Q30 bases (PF) Mean Quality SCore (PF)
lane4_NoIndex_L4 2,765 0.80 0.66 0.15 100.00 187.99 +/-3.20 1.34 +/-0.05 0.95 +/-0.03 51.72 0.15 92.41 36.81
lane8_NoIndex_L8 0 0.80 0.66 186.55 +/-22.53 1.47 +/-0.24 0.77 +/-0.01

IVC Plots

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All Intensity Plots

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Mismatch Graphs

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Mismatch Curves

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Additional Paired Statistics

Barcode-Lane Info Relative Orientation Statistics Insert Size Statistics (for relative orientation R+) Insert Statistics (% of individually uniquely alignable pairs)
Barcode-Lane F-:
>R2 R1>		 F+:
>R1 R2>		 R-:
<R2 R1>		 R+:
>R1 R2<		 Total Median Below-median SD Above-median SD Low thresh. High thresh. Too small Too large Orientation and size OK
lane4_NoIndex_L4 2
(0.33%) 		3
(0.49%) 		21
(3.42%) 		588
(95.77%) 		614 309 100 29 57 377 0
(0.00%) 		1
(0.16%) 		587
(95.60%)

CASAVA-1.8.2