LOCUS NZ_ACVX01000043 24681 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00024, whole genome shotgun sequence. ACCESSION NZ_ACVX01000043 NZ_ACVX00000000 VERSION NZ_ACVX01000043.1 GI:256005825 DBLINK Project: 55637 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. REFERENCE 1 (bases 1 to 24681) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Hemme,C.L. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The draft genome of Clostridium thermocellum DSM 2360 JOURNAL Unpublished REFERENCE 2 (bases 1 to 24681) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Hemme,C.L. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (17-JUL-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000043. URL -- http://www.jgi.doe.gov JGI Project ID: 4085028 Source DNA and Bacteria available from Christopher L. Hemme (hemmecl@ou.edu) Contacts: Christopher L. Hemme (hemmecl@ou.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Annotation by JGI-ORNL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Clostridium thermocellum DSM 2360 Culture Collection ID :: DSM 2360 GOLD Stamp ID :: Gi02594 Isolation Site :: contaminant of Clostridium thermocellum strain LQ8 Isolation Country :: USA Oxygen Requirement :: Anaerobe Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Sporulating Temperature Range :: Thermophile Temperature Optimum :: 60 C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Phenotypes :: Cellulose degrader, Ethanogenic, Ethanol production Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Location/Qualifiers source 1..24681 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene 26..586 /locus_tag="ClothDRAFT_2637" CDS 26..586 /locus_tag="ClothDRAFT_2637" /inference="similar to AA sequence:KEGG:LPC_2301" /note="KEGG: lpc:LPC_2301 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430776.1" /db_xref="GI:256005826" /translation="MEHIIKKYDNVPMYLSANDSVVDFYPKFGFKRVYEKLPVCECAI NNDAMPNKLSYDDPKVWDYVYKRMNFSPKLDCLNSASINIFHIYWGYLKDCIYELPEL DTMVIAEQRGETLKLIGVFSRRDICFSDLVKYLPFINVKKIEFGFMPYWSDVNYIMKE YEKDPLFVRGVRCDLGDFKFPELSIT" gene 1137..1580 /locus_tag="ClothDRAFT_2638" CDS 1137..1580 /locus_tag="ClothDRAFT_2638" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430777.1" /db_xref="GI:256005827" /translation="MRRKLLKILLLIPLIFVIFVASVIIYFIVKPIPIDFSKVSKVEF YECGDISEKFRLLSLENNEDIALINGIFKNAKGDWYGKYNTPGCSFGLQVVFYEGNKK KIVELGTDDCGLIICNEAYYQIPEDDFKILEEFLERRGINIHEYI" sig_peptide 1137..1208 /locus_tag="ClothDRAFT_2638" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.810) with cleavage site probability 0.427 at residue 24" gene 1795..2043 /locus_tag="ClothDRAFT_2639" CDS 1795..2043 /locus_tag="ClothDRAFT_2639" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430778.1" /db_xref="GI:256005828" /translation="MAVDYNAISKIYDKVRSENKSILDLFIEEVKITNTTTILDAARE ITPICCKDLHRPKLTASNLPRYEGESNCKKSEYYSYKR" gene 1991..2179 /locus_tag="ClothDRAFT_2640" CDS 1991..2179 /locus_tag="ClothDRAFT_2640" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: hypothetical protein; K00559 sterol 24-C-methyltransferase" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430779.1" /db_xref="GI:256005829" /translation="MREKAIAKNPNITVTKGDLENIPFEDNYFDFVYMTDAIHHIPDI EMMFKEIGRVSKKVETFA" misc_feature <1991..2161 /locus_tag="ClothDRAFT_2640" /note="S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy)...; Region: AdoMet_MTases; cl12011" /db_xref="CDD:187159" gene complement(2443..3561) /locus_tag="ClothDRAFT_2641" CDS complement(2443..3561) /locus_tag="ClothDRAFT_2641" /EC_number="2.6.1.52" /inference="protein motif:TFAM:TIGR01365" /note="TIGRFAM: phosphoserine aminotransferase; KEGG: sde:Sde_1332 phosphoserine aminotransferase" /codon_start=1 /transl_table=11 /product="phosphoserine aminotransferase" /protein_id="ZP_05430780.1" /db_xref="GI:256005830" /db_xref="InterPro:IPR006271" /translation="MKPTRKPLNPCFSSGPCAKHPGYSIEALKDAPLGRSHRSKLGKA KLYESIVKTKEILKLPEDYKVGIVPGSDTGAFEMLMWNVLGARGVDVLVFESFGKGWA NDIVNELKLENVNIISADYGKLPDISKVNFDNDVVFTWNGTTSGVKVPNGDWIPDDRK GLTLCDATSAVFAMDIPWNKIDAATFSWQKVLGGEAAHGMLILSPRAVERIESYSPSW PLPKVFRLKKKGKLDEAIFEGSTINTPSMLCNEDYLNALRWAESIGGLEKLIARSEEN LKVIENFVEKHPWISFLAKDKSIRSNTSVCLSVDLPEDKIKMLVNLLAEEKVAYDISS YKEAPVGLRIWCGATVEKSDLEILCEWLEWAYNEVANS" misc_feature complement(2953..3429) /locus_tag="ClothDRAFT_2641" /note="Aspartate aminotransferase (AAT) superfamily (fold type I) of pyridoxal phosphate (PLP)-dependent enzymes. PLP combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate, which depending on the reaction, is the...; Region: AAT_I; cd01494" /db_xref="CDD:99742" misc_feature complement(order(2992..2994,3001..3003,3055..3057, 3064..3066,3145..3147,3334..3336,3343..3348)) /locus_tag="ClothDRAFT_2641" /note="pyridoxal 5'-phosphate binding pocket; other site" /db_xref="CDD:99742" misc_feature complement(2473..>3090) /locus_tag="ClothDRAFT_2641" /note="Selenocysteine lyase/Cysteine desulfurase [Posttranslational modification, protein turnover, chaperones]; Region: csdA; COG0520" /db_xref="CDD:30866" misc_feature complement(2992..2994) /locus_tag="ClothDRAFT_2641" /note="catalytic residue; other site" /db_xref="CDD:99742" gene 4272..5747 /locus_tag="ClothDRAFT_2642" CDS 4272..5747 /locus_tag="ClothDRAFT_2642" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: dol:Dole_3113 TPR repeat-containing protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430781.1" /db_xref="GI:256005831" /translation="MKNTFRWTLNKLVVTGACAYVLLILLFMLNSGLVRVSGNVLLNK SLDVLFFTVAAVVGYGVLLLFEKVLNLRMLNKEYARILIVVLMTLITRLVWIHIVDIT PKSDFELYNTLAEAFSRGEAAGGKYVALFPHTFGYPFILATVYRIFSPDKYFALLLNI LFEAGTGVVLYYLGKMVSNWKTGFFAGIICALWPSHVFYSSIVCTEPLYTLLMALLIF VYFKVSVKNKSLLHSCVLYLLLGFLCAAANAIRPMGTLLVAVLGITEVVRIIKKKEGL KQSFAGFVPFAVFLIAYFSFVNLTGMYVSYKIGYNTAKNPIGFNTYVGANINSSGMWN QSDANVLMDFMKQEPFDAQKIHEQLLNLAIQRVKSQGTGNLKLVIKKNMIMWGRDDEV VTYMIAGSGDKTSSLLEVKNSEGLLRYICNFYYYMIVILAFGGLLKQCAKEDNPILMA LLLLFLGIGAIHTVVEVHGRYHYSSMAVFAILAGINNFKIK" sig_peptide 4272..4382 /locus_tag="ClothDRAFT_2642" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.936) with cleavage site probability 0.624 at residue 37" misc_feature 4464..>5441 /locus_tag="ClothDRAFT_2642" /note="conserved hypothetical integral membrane protein; Region: TIGR03766" /db_xref="CDD:163478" gene 5827..8067 /locus_tag="ClothDRAFT_2643" CDS 5827..8067 /locus_tag="ClothDRAFT_2643" /inference="protein motif:PFAM:PF00015" /note="PFAM: chemotaxis sensory transducer; histidine kinase HAMP region domain protein; SMART: chemotaxis sensory transducer; histidine kinase HAMP region domain protein; KEGG: geo:Geob_2268 methyl-accepting chemotaxis sensory transducer" /codon_start=1 /transl_table=11 /product="methyl-accepting chemotaxis sensory transducer" /protein_id="ZP_05430782.1" /db_xref="GI:256005832" /db_xref="InterPro:IPR003660" /db_xref="InterPro:IPR004089" /db_xref="InterPro:IPR004090" /translation="MLKNMTIRTKLLTIVSITVVALLIVTVLSTITINGIFKRVKTTI FDELFWSMSMILNADRDMYQAYVAMRDILSLDENSEEYDELLENYEENIDQVKQRISE TKEMIEKNRENWEETRDSQSGRTVFEFFASFENDFNEWIAEGSKVIETKAVSQVWNEK FEVARNDLDIIGGLIELNAKNQISSIEHTKNSMVVQIVLIDVLALILALAISFIFLRI IGKSLGELSEAAEKIALGDIDVKLDIASKDEIGKLADNFKIMTDSIKEKAEAAEKISK GYLDTDVTVRSDKDILSRSMKAIIENLKHLVDETGILTKAASEGMLKTRGKEDKFEGG YRDIIVGINNTLDAVNAPVLEIQEVLKKVSEGHLNVKVKGDYKGDYAELKNALNNTID TLHSYIEEISRVLTEMAKGNLDVSLSEKYLGDFAHIHEALDTIIVSLNGMIREISVAA GQVAAGSRQVSDSSQVLSQGAAEQASTIEELTCSLEEIAKQTKQNAANASNADKMALT AKNNAIEGNKKMSEMLKAMQDINDASNNISRIIKVIDDIAFQTNILALNAAVEAARAG QYGKGFAVVAEEVRNLAARSANAAKETTQLIEGSIHTVEAGMEIATDTANALNTIVEE ISKAASLVQDISVASNEQATGIAQINQGVSQVSQVIQTNSATAEQSAAASEELSSQAE VLKEMVKKFRLKPNTSLPGYGETDPEVIKYMEDVMEKKENNKKGKKTLRTDFNIDLDN EDFGKY" sig_peptide 5827..5916 /locus_tag="ClothDRAFT_2643" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 0.979) with cleavage site probability 0.847 at residue 30" misc_feature <6487..7344 /locus_tag="ClothDRAFT_2643" /note="Methyl-accepting chemotaxis protein [Cell motility and secretion / Signal transduction mechanisms]; Region: Tar; COG0840" /db_xref="CDD:31182" misc_feature 6487..6618 /locus_tag="ClothDRAFT_2643" /note="Methyl-accepting protein, and Phosphatase (HAMP) domain. HAMP is a signaling domain which occurs in a wide variety of signaling proteins, many of which are bacterial. The HAMP domain consists of two alpha helices connected by an extended linker. The...; Region: HAMP; cd06225" /db_xref="CDD:100122" misc_feature order(6493..6498,6502..6507,6514..6519,6523..6525, 6571..6576,6580..6585,6592..6597,6601..6606,6613..6618) /locus_tag="ClothDRAFT_2643" /note="dimerization interface; other site" /db_xref="CDD:100122" misc_feature 7159..7902 /locus_tag="ClothDRAFT_2643" /note="Methyl-accepting chemotaxis-like domains (chemotaxis sensory transducer); Region: MA; smart00283" /db_xref="CDD:128579" misc_feature 7330..7908 /locus_tag="ClothDRAFT_2643" /note="Taxis toward Aspartate and Related amino acids and Homologs (TarH). The Tar chemoreceptor of Escherichia coli mediates attractant responses to aspartate, maltose, and phenol, repellent responses to Ni2+ and Co2+, and thermoresponses. These...; Region: TarH; cl00144" /db_xref="CDD:153541" gene 8170..9540 /locus_tag="ClothDRAFT_2644" CDS 8170..9540 /locus_tag="ClothDRAFT_2644" /inference="protein motif:TFAM:TIGR00797" /note="TIGRFAM: MATE efflux family protein; PFAM: multi antimicrobial extrusion protein MatE; KEGG: mxa:MXAN_2789 MATE efflux family protein" /codon_start=1 /transl_table=11 /product="MATE efflux family protein" /protein_id="ZP_05430783.1" /db_xref="GI:256005833" /db_xref="InterPro:IPR002528" /translation="MQKETASIKKMSVFALTWPIFIETLLRTMLGNVDTFMLSTYSDD AVGAVGVVSQISYILIMLYNVVSSGTLVLISQYLGAKKKKEASVVAVTSIAGSLIFGL FVGLAVFLFRSQILTFLNLPPELMGYAMTFLGIVGGFSFTQALIATLSAIIRSYGNTR ITMYISVGMNILNIIGNSIFLYGLLGAPKMGVTGVAIATVISQAVGVVAMLIVMLTGL NQKFSFRDLVPLPWEILRDILKIGLPSAGEGIAYEASQLTITRIITVLGKVALTTRVY TLNIMYFVMVFSVAVGQGTQIVVGHLVGAGDNEKAYKTCIKSLRYAVVVAIILAGIVS FFSEQLLGIFTDDRAIIEMGSKLLLIAVILEPGRVFNIVIINSLRAAGDARFPVIMGI ISMWGIGVLLSYFLGVACGLGLIGVWIAFASDEWFRGIAMLLRWRSRVWYKMALVKNQ NIEMPA" misc_feature 8203..9522 /locus_tag="ClothDRAFT_2644" /note="Na+-driven multidrug efflux pump [Defense mechanisms]; Region: NorM; COG0534" /db_xref="CDD:30880" misc_feature 8224..8700 /locus_tag="ClothDRAFT_2644" /note="MatE; Region: MatE; pfam01554" /db_xref="CDD:144956" misc_feature 8899..9381 /locus_tag="ClothDRAFT_2644" /note="MatE; Region: MatE; pfam01554" /db_xref="CDD:144956" gene complement(9640..10257) /locus_tag="ClothDRAFT_2645" CDS complement(9640..10257) /locus_tag="ClothDRAFT_2645" /inference="protein motif:TFAM:TIGR02899" /note="KEGG: noc:Noc_1373 hypothetical protein; TIGRFAM: spore coat assembly protein SafA; PFAM: SCP-like extracellular; Peptidoglycan-binding LysM; SMART: Peptidoglycan-binding LysM" /codon_start=1 /transl_table=11 /product="spore coat assembly protein SafA" /protein_id="ZP_05430784.1" /db_xref="GI:256005834" /db_xref="InterPro:IPR002482" /db_xref="InterPro:IPR014044" /db_xref="InterPro:IPR014248" /db_xref="InterPro:IPR014258" /translation="MKRKNILVLLLLLILIFTTSTVFAQTQVYTVKPGDTMWKIAVKY QIGISEIIAANPQIKNPNLIYPGQKINIPNIDDIKAQENEVIRLVNVERAKKGLPALK ANWQLSRVARYKSEDMANKGYFSHTSPTYGSPFKMMEDFGIKFTAAGENIAMGQQTAR DVMNAWMNSPGHRNNILSPSFTEIGVGLAKGPNGRLYWTQMFIKK" sig_peptide complement(10183..10257) /locus_tag="ClothDRAFT_2645" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.998 at residue 25" misc_feature complement(10039..10179) /locus_tag="ClothDRAFT_2645" /note="Lysin domain, found in a variety of enzymes involved in bacterial cell wall degradation. This domain may have a general peptidoglycan binding function; Region: LysM; cd00118" /db_xref="CDD:29017" misc_feature complement(order(10129..10131,10138..10140,10153..10155, 10162..10164,10171..10173)) /locus_tag="ClothDRAFT_2645" /note="putative peptidoglycan binding site; other site" /db_xref="CDD:29017" misc_feature complement(9652..10014) /locus_tag="ClothDRAFT_2645" /note="SCP_bacterial: SCP-like extracellular protein domain, as found in bacteria and archaea. The wider family of SCP containing proteins includes plant pathogenesis-related protein 1 (PR-1), CRISPs, mammalian cysteine-rich secretory proteins, and allergen 5...; Region: SCP_bacterial; cd05379" /db_xref="CDD:88558" gene 10423..11193 /locus_tag="ClothDRAFT_2646" CDS 10423..11193 /locus_tag="ClothDRAFT_2646" /inference="protein motif:PFAM:PF06738" /note="PFAM: protein of unknown function DUF1212; KEGG: sfr:Sfri_0394 protein of unknown function DUF1212" /codon_start=1 /transl_table=11 /product="protein of unknown function DUF1212" /protein_id="ZP_05430785.1" /db_xref="GI:256005835" /db_xref="InterPro:IPR010619" /translation="MRVNGMKTNQVIEVALKAGEILLTGGAEIYRVEDTVTRICGSYN VRAECFVLPTGIFITVIDSEGNPVSIIRRIRKRTVNLEQVEKVNSFSRSLKDRTLSYQ EAMAGLKDIAALDGYGYVLKLIVAGIAPFVYALLFRGTVLEACVSFLIGIVIYAIKEK ITQAGVFEFFEYFASGVAAGALSTLAIKLFPFMDLYRIIIASIIILLPGLAITNAMKD ALYGDIVSSMFRLTEAVFVAAAVGVGVAIALSVGLRWI" misc_feature 10474..11007 /locus_tag="ClothDRAFT_2646" /note="Protein of unknown function (DUF1212); Region: DUF1212; cl01118" /db_xref="CDD:186342" gene 11213..11653 /locus_tag="ClothDRAFT_2647" CDS 11213..11653 /locus_tag="ClothDRAFT_2647" /inference="protein motif:COG:COG3610" /note="KEGG: sfr:Sfri_3085 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430786.1" /db_xref="GI:256005836" /translation="MVEIFLAFLGSICPGVIYNVEKRNLMWVGFGGMLGWITYRTFNE ITKSLVLASFLGAVIVGIYSESVARLLKAPATIFSVPGIIPLVPGIAAYSTIQHVAEN QLYEAASKGIETMSGAGAIAFGILLASAVFRLTKKLKKRGPNPS" misc_feature 11213..>11557 /locus_tag="ClothDRAFT_2647" /note="Protein of unknown function (DUF1212); Region: DUF1212; cl01118" /db_xref="CDD:186342" gene complement(11699..11893) /locus_tag="ClothDRAFT_2648" CDS complement(11699..11893) /locus_tag="ClothDRAFT_2648" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430787.1" /db_xref="GI:256005837" /translation="MTEENKLETLDTGKAPEEKETARDTSEALQASTIDRSSCFSPFN EAAVILFFILVFLLLFYRNR" gene complement(11980..12426) /locus_tag="ClothDRAFT_2649" CDS complement(11980..12426) /locus_tag="ClothDRAFT_2649" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430788.1" /db_xref="GI:256005838" /translation="MHGQSNLSLNCDFAGMDSIYELEMLHLKDMGNYIYNFLLPNLQK SYKRAKQYLAGNTRKNIYSMQKYLADLIDDYDFVKLSINEDIGSEYFTKYEALFLLTE SLNMIYFFCAVAKSKIKNDNPESRLILRNLMKLTSEVHKEINCLME" gene 12782..14767 /locus_tag="ClothDRAFT_2650" CDS 12782..14767 /locus_tag="ClothDRAFT_2650" /inference="protein motif:PFAM:PF00986" /note="PFAM: DNA gyrase subunit B domain protein; TOPRIM domain protein; ATP-binding region ATPase domain protein; DNA topoisomerase type IIA subunit B region 2 domain protein; SMART: DNA topoisomerase II; KEGG: hypothetical protein ; K02470 DNA gyrase subunit B" /codon_start=1 /transl_table=11 /product="DNA gyrase subunit B domain protein" /protein_id="ZP_05430789.1" /db_xref="GI:256005839" /db_xref="InterPro:IPR000565" /db_xref="InterPro:IPR001241" /db_xref="InterPro:IPR002288" /db_xref="InterPro:IPR003594" /db_xref="InterPro:IPR006171" /db_xref="InterPro:IPR013506" /translation="MSKEKQNKAERYGNTSIKSLKGAERVRLRPAVIFGSDGIEGCQH SFFEILSNSIDEAREGFGKVIEVTRFKDRSIMVKDYGRGIPLDYNENEKRYNWELVFC ELYAGGKYKNNSGENYEFSLGLNGLGSCATQYSSEYMDVTVFRDGMRYDLHFEKGENV GGLKKQKWDYESTGTIIKWRPDLEVFTDINIPLEYYTEVLRKQAVVNAGLKFILYDEE SDRVFEYCYENGIVDYIKEVSGDKAFTDIQYYETSTRGRDREDKPEYKLKMQIAFCFN NEVNLLEYYHNSSFLEYGGAPDKAVKNAFVYVIDKFLKSQGKYNKDESRITFQDIQDS LILVSNSFSTITSYENQTKKSITNKFIQEAMTEFLKEKLEVYFIENKVECTKILEQVL VNKRSRETAEKTRVNIKKKLSGNVDISNRVKKFVDCRSKDVNKREIYIVEGDSALGSC KLARDAEFQAIIPVRGKILNCLKADYDSIFKNEIIVDLLKVLGCGVEIKSKHNRELST FDLNNLRWSKIIICTDADVDGYQIRTLILAMLYRLVPTLIQVGKVFIAESPLFEIRTK DKTYFAYTDKEKNEIVSKLKGKYTILRSKGLGENDPDMMWQTTMNPETRRLIQVVPDD IKKTEEFFDLFLGDNLPGRKSYIEEHGHKYLDMIDVM" misc_feature 12800..14761 /locus_tag="ClothDRAFT_2650" /note="Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit [DNA replication, recombination, and repair]; Region: GyrB; COG0187" /db_xref="CDD:30536" misc_feature 13466..13996 /locus_tag="ClothDRAFT_2650" /note="MutL_Trans: transducer domain, having a ribosomal S5 domain 2-like fold, conserved in the C-terminal domain of type II DNA topoisomerases (Topo II) and DNA mismatch repair (MutL/MLH1/PMS2) proteins. This transducer domain is homologous to the second...; Region: TopoII_MutL_Trans; cl02783" /db_xref="CDD:155101" misc_feature 13838..13840 /locus_tag="ClothDRAFT_2650" /note="ATP binding site; other site" /db_xref="CDD:73195" misc_feature 14087..14452 /locus_tag="ClothDRAFT_2650" /note="Topoisomerase-primase domain. This is a nucleotidyl transferase/hydrolase domain found in type IA, type IIA and type IIB topoisomerases, bacterial DnaG-type primases, small primase-like proteins from bacteria and archaea, OLD family nucleases from...; Region: TOPRIM; cl00718" /db_xref="CDD:186161" misc_feature order(14105..14110,14117..14119,14351..14353,14357..14359, 14363..14365) /locus_tag="ClothDRAFT_2650" /note="active site" /db_xref="CDD:173773" misc_feature order(14105..14107,14351..14353) /locus_tag="ClothDRAFT_2650" /note="metal-binding site" /db_xref="CDD:173773" misc_feature 14531..14725 /locus_tag="ClothDRAFT_2650" /note="DNA gyrase B subunit, carboxyl terminus; Region: DNA_gyraseB_C; pfam00986" /db_xref="CDD:144542" gene 14842..17025 /locus_tag="ClothDRAFT_2651" CDS 14842..17025 /locus_tag="ClothDRAFT_2651" /EC_number="5.99.1.3" /inference="protein motif:PRIAM:5.99.1.3" /note="KEGG: pca:Pcar_0005 DNA gyrase, A subunit; PFAM: DNA gyrase/topoisomerase IV subunit A; DNA gyrase repeat beta-propeller; SMART: DNA gyrase/topoisomerase IV subunit A" /codon_start=1 /transl_table=11 /product="DNA topoisomerase (ATP-hydrolyzing)" /protein_id="ZP_05430790.1" /db_xref="GI:256005840" /db_xref="InterPro:IPR002205" /db_xref="InterPro:IPR006691" /translation="MSTNNLIEQRIVDTLEKNYMPYAMSVIVSRAIPEIDGLKPSHRK LLYTMYKMGLLTGEKTKSANVVGQTMKLNPHGDMAIYETLVRLTRGNGALLLPYVDSK GNFGKHYSRDMKYAAPRYTEVKLDKICEELFKDIDKDTVDFVDNYDGTMKEPKLLPAT FPTILVNANHGIAVGMASSICSFNLVEVCNAAVEFIKDENTDLVGILKAPDFPTGGQL IYNEREMREIYEKGRGTFKLRAKYRYDKANSCIEIYEIPYTTTVEAIIDSIADLVKSN KIRDISDVRDETDLNGLKIALDIKRNADPDTLMNKLYKLTPLEDSFSCNFNILINGRP MVMGVRGILKEWVNFRMDCIKRQTLYDIDKKTQKLHILLGLEKILLDIDKAIKIIRNT EEEAMVVPNLMEGFGIDQAQAEFVAEIKLRNLNKEYILKRVSEIESLKKEIAELNELY GSEKKIKKLIIKQLEEIAKKYGQPRRTEIVMEEHIEEITHEHLIEDYNLKLFLTKQNY LKKIPLTSLRTSPEHKLKEGDFIVQELETHNKADLLLFSNRQNVYKMKIYEIEDCKAS SLGEFLSNVLSLEEGEEIIHMVATDDYKGYMIFGFENGKVAKIVLESYATKTNRKKLA NAYNGLSRLVYIGHILEDEELVAFSSLNKVLIFNTSNINPKTTRDSQGVQVLKSKKGS VMTHIKKISEVSFTDPDYYRTKNIPAIGCYLKDEDVEDNQIKLEL" misc_feature 14923..16197 /locus_tag="ClothDRAFT_2651" /note="DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in...; Region: TOP4c; cd00187" /db_xref="CDD:29149" misc_feature order(14923..14946,14947..15003,15016..15018,15019..15051, 15052..15108,15112..15159,15169..15171,15175..15183, 15184..15228,15232..15303,15310..15312) /locus_tag="ClothDRAFT_2651" /note="CAP-like domain; other site" /db_xref="CDD:29149" misc_feature 14929..16188 /locus_tag="ClothDRAFT_2651" /note="DNA gyrase/topoisomerase IV, subunit A; Region: DNA_topoisoIV; pfam00521" /db_xref="CDD:144201" misc_feature 15199..15201 /locus_tag="ClothDRAFT_2651" /note="active site" /db_xref="CDD:29149" misc_feature order(15979..15987,15994..16005,16027..16032,16081..16131) /locus_tag="ClothDRAFT_2651" /note="primary dimer interface; other site" /db_xref="CDD:29149" misc_feature <16342..16923 /locus_tag="ClothDRAFT_2651" /note="Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit [DNA replication, recombination, and repair]; Region: GyrA; COG0188" /db_xref="CDD:30537" gene 17200..17559 /locus_tag="ClothDRAFT_2652" CDS 17200..17559 /locus_tag="ClothDRAFT_2652" /inference="ab initio prediction:Prodigal:1.4" /note="KEGG: scl:sce4225 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430791.1" /db_xref="GI:256005841" /translation="MYKGQVRSPALVIILSIVTCGIYAWYWIYKMSEELQRATGKSTI SPGVELLLCIVTCGIYPIYWFYKYGKMITEAQEQAGLNAEDNAVLYLLLAIFGLGVVN MALMQSAVNKIWEQDII" gene 17617..17901 /locus_tag="ClothDRAFT_2653" CDS 17617..17901 /locus_tag="ClothDRAFT_2653" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430792.1" /db_xref="GI:256005842" /translation="MTRAYLSLIKFDLTSAFYYHPLFLLPGLVAVVVVFRNNRFLGRL YRNNILWCCVLAVFVLVWIVRLVLFFPDIPPMNYNSQALIPRLLGLFKIE" gene 18064..20046 /locus_tag="ClothDRAFT_2654" CDS 18064..20046 /locus_tag="ClothDRAFT_2654" /inference="protein motif:TFAM:TIGR00631" /note="KEGG: gur:Gura_0291 excinuclease ABC subunit B; TIGRFAM: excinuclease ABC, B subunit; PFAM: helicase domain protein; UvrB/UvrC protein; type III restriction protein res subunit; SMART: DEAD-like helicase ; helicase domain protein" /codon_start=1 /transl_table=11 /product="excinuclease ABC, B subunit" /protein_id="ZP_05430793.1" /db_xref="GI:256005843" /db_xref="InterPro:IPR001650" /db_xref="InterPro:IPR001943" /db_xref="InterPro:IPR004807" /db_xref="InterPro:IPR006935" /db_xref="InterPro:IPR014001" /db_xref="InterPro:IPR014021" /translation="MHKFKLVSDYKPCGDQPEAIDKLVEGINRGYRGQTLLGVTGSGK TFTMANVIERVQKPTLVIAHNKTLAAQLCSEFKEFFPNNCVEYFVSYYDYYQPEAYIP ATDTYIEKDSSINDEIDKLRHSATAALFERRDVIIVASVSCIYGLGDPEDYTDLMLSL RPGMIKDRDEIIRKLVDIQYERNEIDFKRGKFRVRGDILEIFPASSSDKVIRVEFFGE EIDRITEVDSLTGEITGVCSHVAIFPASHYATTKAKMQRAIASIEQELEERVRELKSQ GKLLEAQRLEQRTRYDLEMMQEIGFCQGIENYSRHISGRAPGSPPFTLIDYFPKDFLL IIDESHVTIPQIGAMYNGDRSRKESLVEYGFRLPSAFDNRPLTFEEFEKKINQVIFVS ATPAKYEREHSQQIVEQIIRPTGLLDPEIVVKPVKGQIDDLIGEISERVQKNQRVMIT TLTKKMAEDLTDYLRELDFKVEYLHSDIDTIERMEIIRNLRLGVFDVLVGINLLREGL DIPEVSLVAILDADKEGFLRSETSLIQTIGRAARNVEGKVIMYADTITDSMRRAIDET NRRRKIQSEYNQKHGITPKSVQKGIRDVIEITKVAEEDAKYFIRGDEDSMDKDEVLDL IEKLTNEMKAAAAELQFERAAELRDKIAELKKKIGA" misc_feature 18064..20037 /locus_tag="ClothDRAFT_2654" /note="excinuclease ABC subunit B; Provisional; Region: PRK05298" /db_xref="CDD:180000" misc_feature 18157..>18357 /locus_tag="ClothDRAFT_2654" /note="DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region; Region: DEXDc; cd00046" /db_xref="CDD:28927" misc_feature 18184..18198 /locus_tag="ClothDRAFT_2654" /note="ATP binding site; other site" /db_xref="CDD:28927" misc_feature 19321..19716 /locus_tag="ClothDRAFT_2654" /note="Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may...; Region: HELICc; cd00079" /db_xref="CDD:28960" misc_feature order(19417..19428,19486..19491,19564..19572) /locus_tag="ClothDRAFT_2654" /note="nucleotide binding region; other site" /db_xref="CDD:28960" misc_feature order(19588..19590,19666..19668,19678..19680,19687..19689) /locus_tag="ClothDRAFT_2654" /note="ATP-binding site; other site" /db_xref="CDD:28960" misc_feature 19711..19842 /locus_tag="ClothDRAFT_2654" /note="Ultra-violet resistance protein B; Region: UvrB; pfam12344" /db_xref="CDD:152779" gene complement(20072..21118) /locus_tag="ClothDRAFT_2655" CDS complement(20072..21118) /locus_tag="ClothDRAFT_2655" /inference="protein motif:PFAM:PF06725" /note="PFAM: 3D domain protein; KEGG: tdn:Suden_1285 MltA" /codon_start=1 /transl_table=11 /product="3D domain protein" /protein_id="ZP_05430794.1" /db_xref="GI:256005844" /db_xref="InterPro:IPR010611" /translation="MGNTIGNIFNCPDLNRVHSRSREYLSEYFQEEEINSLNYPEILE EVLMNAAQLKLNYPEILEEIIRKVDSAVLSSKSPSILERVKCCSQSILSKKDLDLKVV AAGSLMTFLLTCAGNNILNSITEKTASHVHNFASSTWVSPDSAKLSSLVKESMPAKTE GALASVSAVNTANISFAEIKKSIESKNTSKTQSTPVSRSKLTAKSNSATEAKSAQTQV KRTKVEIADIMPQCKRIINMTATAYDLSYKSCGKTREHPAYGITASGTRATVGRTVAV DPSVIPLGTRVYISFPVAYSHLDGIYIAEDTGSLIKGNKIDIFFGEDKPGETVIYNKA MKFGLQEVVVYVLD" misc_feature complement(20075..20305) /locus_tag="ClothDRAFT_2655" /note="3D domain; Region: 3D; cl01439" /db_xref="CDD:154402" gene 21583..24411 /locus_tag="ClothDRAFT_2656" CDS 21583..24411 /locus_tag="ClothDRAFT_2656" /inference="protein motif:TFAM:TIGR00630" /note="KEGG: gme:Gmet_0128 excinuclease ABC subunit A; TIGRFAM: excinuclease ABC, A subunit; PFAM: ABC transporter related; SMART: AAA ATPase" /codon_start=1 /transl_table=11 /product="excinuclease ABC, A subunit" /protein_id="ZP_05430795.1" /db_xref="GI:256005845" /db_xref="InterPro:IPR003439" /db_xref="InterPro:IPR003593" /db_xref="InterPro:IPR004602" /db_xref="InterPro:IPR017871" /translation="MKKDYIVVKGAREHNLKNIDVKIPRDKFVVITGLSGSGKSSLAF DTIYAEGQRRYVESLSSYARQFLGQMEKPDVDYIDGLSPAIAIDQKTTSRNPRSTVGT VTEIYDYLRLLFARIGTPHCYLCGREISQQTVDQMVDRIMEFEEGTRIQLLAPVVRGR KGEYHKLIEDIKKEGYVRIRVDGEVVDVNDPVNLDKNKKHNIEIVVDRLIVRPGIQKR LTDSIETVLRLSNGILVVDVIGGKEMLLSQNFACTECNVSMEEITPRMFSFNNPYGAC PECTGLGSLMRIDPDLVIPDKKLSLAQGAVRASGWNIANDESYARMYIDALAKHYNFS VDTPVEELPPHILDIILYGTNGEKIKIEYERENEKGTFMASFPGIINSMERRYKETTS EVMKQYYENFMSNIPCPVCKGARLKKESLAVTIGGKNIYEVCCLSIGEAKEFFANLNL TERQQLIARQILKEINARLGFLVDVGLDYLTLARAAGTLSGGEAQRIRLATQIGSGLM GVIYILDEPSIGLHQRDNDRLLRSLKKLRDLGNTLLVVEHDEDTMYASDYIIDLGPGA GSHGGQIVAEGTVEEIKQNPNSVTGEYLSGRKKIEVPKERRKPNGKWLEIIGARENNL KNINVRIPLGVFTCITGVSGSGKSSLINEILYKRLAAELNRASVKPGEHDLIKGIEYL DKVIDIDQSPIGRTPRSNPATYTGVFDFIREIFANTTEAKTRGYKAGRFSFNVKGGRC EACAGDGINKIEMHFLPDIYVPCEVCKGKRYNRETLEVRYKGKNIAEVLDMTVEEALE FFKNIPRIHKKIETLYDVGLGYIKLGQSSTTLSGGEAQRVKLATELSRKSTGKTIYIL DEPTTGLHMADVHRLVGILHRLVEAGNSVVVIEHNLDVIKTADYIIDLGPEGGSGGGL VVAEGTPEEVAKVENSYTGQFLKKVLST" misc_feature 21583..24402 /locus_tag="ClothDRAFT_2656" /note="excinuclease ABC subunit A; Reviewed; Region: uvrA; PRK00349" /db_xref="CDD:178984" misc_feature 21598..>21936 /locus_tag="ClothDRAFT_2656" /note="The excision repair protein UvrA domain I; Nucleotide excision repair in eubacteria is a process that repairs DNA damage by the removal of a 12-13-mer oligonucleotide containing the lesion. Recognition and cleavage of the damaged DNA is a multistep...; Region: ABC_UvrA_I; cd03270" /db_xref="CDD:73029" misc_feature <22732..23313 /locus_tag="ClothDRAFT_2656" /note="The excision repair protein UvrA domain I; Nucleotide excision repair in eubacteria is a process that repairs DNA damage by the removal of a 12-13-mer oligonucleotide containing the lesion. Recognition and cleavage of the damaged DNA is a multistep...; Region: ABC_UvrA_I; cd03270" /db_xref="CDD:73029" misc_feature 23422..24291 /locus_tag="ClothDRAFT_2656" /note="The excision repair protein UvrA domain II; Nucleotide excision repair in eubacteria is a process that repairs DNA damage by the removal of a 12-13-mer oligonucleotide containing the lesion. Recognition and cleavage of the damaged DNA is a multistep...; Region: ABC_UvrA_II; cd03271" /db_xref="CDD:73030" ORIGIN 1 aggcagaggg ttgatgcgga tattgatgga gcatatcata aaaaaatacg ataatgttcc 61 gatgtactta tcggcaaatg acagtgtggt ggatttctat ccgaagtttg gctttaagcg 121 tgtatatgaa aaactgccgg tttgcgagtg tgcaataaat aatgatgcta tgccgaataa 181 actcagctat gacgacccta aagtatggga ttatgtttac aaacgcatga atttttcgcc 241 aaagcttgat tgcttaaatt ctgccagcat aaatattttt cacatatact ggggatatct 301 taaggattgt atttacgagc ttccggagct tgacactatg gttatcgcag agcaaagagg 361 ggagactctt aaacttattg gagttttctc aaggagagat atttgtttct ctgacctggt 421 taagtatctg ccgtttatca atgttaaaaa gattgaattt ggttttatgc cctattggtc 481 tgatgtaaat tatataatga aagaatatga aaaggacccc ttgtttgtca gaggagtgcg 541 ctgcgattta ggcgatttta agttcccgga attgtctata acatagctgt aaatttgcaa 601 atgaaacgta attcattgca aaattaatct ttttgcataa ctttttcagg ctctgtattt 661 taaaatatgg agcccattct tttgctgata tcatgaatgc cgcgcactaa tgttcattac 721 gccaagaagg ataccagttt aaagggttga agcgtgttgc agaaggcaaa ggacttctgg 781 cggattagtc tattgacata attccatttt aatttatggt ttgttagaaa taaattactt 841 tctagcttct ttcgaaaaat aaataatggt ggtgggataa aaatgatgaa tataaaaaga 901 ttttttaggc atcaaaaaag gatttgataa aagaaaaaaa tactataaaa ttaaaatata 961 agaaatgaaa gattatgtgt aaattgtcag ttccaaggtc gaggatttga aggataactt 1021 atgtatagtg ccactataag ggaatctatt gattatatta tataaaaatt attatataaa 1081 aatgagatgt ttgcctggat gttttgtaga ttagttaaac ttataggagg gaaatagtga 1141 gaaggaagtt attaaagata ttattgctaa ttcctttaat ttttgttata tttgttgctt 1201 ctgttattat ttattttata gtaaagccca ttccgataga cttttccaaa gttagtaaag 1261 ttgagtttta tgaatgtgga gatataagcg agaaatttag attgctgtca ttggaaaata 1321 atgaggatat tgctctgata aatggaatat tcaaaaatgc aaaaggtgat tggtatggca 1381 agtataatac tcctggatgt tcctttggat tacaagtggt attttatgag ggaaataaga 1441 aaaagattgt tgaacttgga actgatgact gtggacttat aatttgtaat gaggcatatt 1501 accaaatacc tgaagatgat ttcaaaattt tggaagaatt tttagaacgg cgaggtataa 1561 atattcacga atatatttaa ttcccgatta ataccttgaa atgccaaagc aagagcgggg 1621 gacagttcag taatttttag tgccccgttt tttatttttc ctgtggcgga aaaataattg 1681 cacattttgt aacagcatca taaaaatttt tcccatatta taaagtaatc attgtatatt 1741 tatacattta attgtataat tattcatata aactttaaag ggaagtgaaa tcggatggcg 1801 gtagattaca atgcgatatc aaaaatatat gataaagtaa ggtcggaaaa caaaagtatt 1861 ttggatttat tcattgaaga ggtcaaaatt acaaacacaa caacaatttt ggatgcggca 1921 cgggaaatta caccaatatg ctgcaaagac ttacacaggc caaagcttac ggcgtcgaac 1981 cttccgaggt atgagggaga aagcaattgc aaaaaatccg aatattacag ttacaaaagg 2041 tgaccttgaa aacatacctt ttgaggataa ctactttgat tttgtgtaca tgacggatgc 2101 cattcatcat attcctgata ttgagatgat gtttaaagaa atcggaagag tttcgaaaaa 2161 agtggaaacc tttgcatagc aacccagtcc catgaacaaa tcgacaacag gttttacgta 2221 aaatactttc ttatttgctt tgcatagctt tggcagtttt tttgacggca gttatttaca 2281 gaatatttaa aagctatggc attgatttga tggaaataaa agtatagcga aaatcgggga 2341 aaattaatgc ttatcatttt agtgaaaatt tcgtgccgtt aaattgataa cacccaccgc 2401 aataggcatt gagccggctg caggtgggtg tatttcagat ttttatgaat ttgcgacttc 2461 attgtatgcc cattccaacc attcgcataa aatttccagg tcggattttt caacggttgc 2521 accgcaccat attctaaggc ctacaggagc ttctttgtat gaagaaatgt cataggccac 2581 tttttcctcg gcgagcaggt taacaagcat tttgatttta tcctccggaa gatcaacgct 2641 caggcaaacg cttgtattgg aacgtatgga tttatccttt gcaaggaaac ttatccatgg 2701 atgcttttca acaaagtttt caattacctt taagttttcc tcacttctcg caataagctt 2761 ttcaagtcct cctatggatt ccgcccagcg gagagcattg aggtagtctt cattacacag 2821 catggacggt gtgttgattg tggaaccttc gaaaatagcc tcgtcaagtt tgcctttctt 2881 tttgagtctg aatactttgg gaagcggcca tgacggtgaa taggattcta ttctttccac 2941 tgcccttggg gacaatatca gcattccgtg agcagcttca ccgcccaata ccttttgcca 3001 tgagaaggtt gccgcgtcaa ttttgttcca cggaatatcc attgcaaaaa cggcggaagt 3061 ggcatcgcag agggttaacc cctttctgtc gtcaggaatc cagtctccgt tcggcacttt 3121 gacaccgctg gttgttccgt tccatgtaaa taccacgtca ttgtcgaagt taaccttgct 3181 gatatcgggc aacttaccgt aatcggcgga aattatattg acattttcca gtttaagctc 3241 atttacgatg tcatttgccc agcctttacc aaaggattca aagacaagta catcaacacc 3301 tcttgcgccg aggacattcc acataagcat ttcaaacgct cccgtatcgg aaccgggtac 3361 tattcccact ttgtaatctt ccggaagttt gagtatttcc tttgttttga caatggattc 3421 gtaaagtttg gcttttccaa gtttgctcct gtgtgacctt ccaagcggtg catctttaag 3481 ggcttcaatt gaatatccgg ggtgcttcgc gcatggtccg gatgaaaaac aagggtttaa 3541 aggttttctg gtcggcttca ttttattctc tcctcacttc gttttggttt tatttcgatt 3601 gtggttgaaa taatacataa aatatataaa caaaaaaact ctcaaccctg aaaatgatca 3661 gggacgagag attttatccg cggtgccacc caaattagcc ggatataata atccgactca 3721 cttttgcgca caataaccgg tgctacggtt tgattcatca tcaacgcctc aggggtggaa 3781 tcaacaaagc ctcaggttga tttgcaccaa acatcaactc tcttatacag ggcacttgtc 3841 actttccccg tcacgggctt gtaaaatatt aaactatcaa attttacctt caatatttac 3901 tataatttta ttatattttg cgtatcaatg caatagggta tgttaaattt ttaaccggat 3961 tttacaaata ataaattcag aaaaaaggga ttggaaattt agataagtat aggaattaaa 4021 aagcaaacta ttttcgaata aactgtaaac tgttgaaagt ataccgaaac tggaacttaa 4081 aataaaattt tcccggataa aaataaattt tattttccac ttgatattgc aaaaaacata 4141 tagtaacctt ctagttgaaa gaattgcagt atgtcttttc tgttatatta atcttgtatt 4201 aatcttgtat taatcttggg tattgaatgc atgattaaat gatacaattt tggctttaag 4261 gagagagggc tttgaaaaat acttttagat ggacattgaa caaactggtt gttacaggag 4321 catgtgcgta tgttctttta attctgctgt ttatgttaaa ttccggtttg gttcgggtgt 4381 ccgggaatgt tttgctgaat aagtctttgg atgtgttgtt tttcacggtg gcggcagttg 4441 taggttacgg agtgctactg ctctttgaaa aagttttaaa tttgcgtatg ttgaataagg 4501 agtatgcaag gatactgatt gtggttttaa tgaccctgat tacgaggctt gtttggatac 4561 atattgtaga tattactccc aaaagtgatt ttgagctgta caatactttg gcggaggcat 4621 tctcgcgggg agaggccgcc ggaggcaagt atgtggctct ttttccccat acatttggtt 4681 atccctttat actggcaacg gtatatcgaa ttttttctcc tgacaagtat tttgctttgc 4741 ttttaaatat tttgtttgaa gcaggcacgg gtgttgtttt atattatctt gggaagatgg 4801 tctcaaactg gaaaacaggt ttctttgcag gcattatatg tgctttatgg ccttcccatg 4861 tgttttattc ttcaattgtt tgcaccgagc cgctgtatac attattaatg gcgcttttga 4921 tatttgtata ttttaaggtg tcagtcaaaa ataaaagctt attgcattcc tgcgttttgt 4981 atcttttgct cgggttttta tgtgcggcgg ccaatgcgat ccgtcctatg ggtactctgc 5041 ttgttgcagt tctgggaata actgaggttg tgagaataat taagaaaaag gaggggttaa 5101 aacaaagttt tgcaggattt gtgccctttg ccgtattctt aatagcatat ttctcttttg 5161 ttaatttgac aggcatgtat gtttcctata aaattggcta caatacggct aaaaatccca 5221 taggttttaa tacttatgtc ggcgccaata ttaattccag cgggatgtgg aaccagagtg 5281 atgccaatgt gctaatggat tttatgaagc aggagccttt tgatgcccaa aaaatacacg 5341 aacagttgct caatctggca attcaaaggg tgaaaagcca gggaacgggt aatttgaagc 5401 ttgtaataaa aaagaatatg atcatgtggg gcagggacga tgaagttgta acctatatga 5461 ttgccggaag cggtgataaa acctcatcgt tattggaagt aaagaactct gaaggtcttt 5521 tgaggtatat ttgcaacttc tattactaca tgatagttat attggcattt ggtggccttt 5581 tgaagcagtg tgctaaagag gataatccaa tccttatggc tttactgctg ctgtttcttg 5641 gaattggtgc tatacatacc gttgttgagg tacatggcag gtatcattat tcatctatgg 5701 ctgtttttgc catacttgcc ggaataaaca attttaaaat taaatagtaa aaaatcagac 5761 ttggcactcc gataagttat ataagtcgtg caatacaaaa aatgatttaa ggagttgact 5821 aagtctatgc taaagaacat gaccataaga actaaactgt tgacgatcgt atccattaca 5881 gttgtggcac ttctgatagt aacagtcctg tccaccatca caatcaacgg tattttcaag 5941 agagtaaaga ccactatttt tgatgagctt ttctggtcga tgtctatgat tttaaatgct 6001 gatagagata tgtaccaggc ctatgtggca atgagggata ttcttagttt agatgaaaac 6061 agtgaagaat atgatgagtt acttgagaat tacgaagaaa atatagatca ggttaaacaa 6121 cgaatatctg agacaaaaga aatgatagaa aaaaacaggg agaattggga ggagacaaga 6181 gacagccaaa gtggaagaac agtttttgag ttttttgcgt cttttgaaaa cgattttaat 6241 gagtggattg cggaaggcag caaggtgatt gagactaaag ctgtatccca ggtctggaac 6301 gagaaatttg aagtggccag aaatgacctg gacataattg ggggattgat tgagcttaat 6361 gcaaaaaatc aaatatcttc gattgaacat acaaaaaaca gcatggtcgt gcaaatagtg 6421 ttaattgatg ttttggcctt gattcttgcg ctggcaattt catttatctt cttaagaatt 6481 atagggaaat ctcttggaga attgagtgaa gcagctgaga aaatagcttt gggagatata 6541 gatgtcaaac tggacattgc ttcgaaggat gaaataggga aacttgccga caactttaaa 6601 ataatgactg atagtataaa ggagaaagcg gaagctgctg aaaaaatatc taaaggatat 6661 cttgatactg atgttactgt cagatcggac aaggatattt tatccaggag tatgaaagcc 6721 attattgaaa atttgaaaca tcttgtggat gaaacgggaa ttcttacaaa agccgcttcc 6781 gaaggaatgt tgaaaacaag aggaaaggaa gataagtttg agggtggcta ccgtgacata 6841 attgtaggta ttaacaatac ccttgatgct gtaaatgcac ctgttttgga aattcaggaa 6901 gttttaaaga aggtatctga aggacacttg aatgtgaaag ttaaagggga ttacaaaggg 6961 gattatgcag aacttaagaa tgcattgaac aacaccattg acacattgca ctcatatatt 7021 gaagaaatat ccagggtact tactgaaatg gcaaaaggaa atctggatgt aagcctgtct 7081 gaaaaatatc tgggtgactt tgcacatatc catgaagccc tggacaccat tattgtatcg 7141 ctaaacggaa tgatacgtga aatttctgtt gcggcaggac aggtggcggc aggctcaagg 7201 caggtgtcgg attcaagcca ggttctgtcc cagggagctg cagagcaggc aagcactatt 7261 gaggagctta cgtgttccct tgaggagatt gcaaaacaga caaaacagaa tgcggctaat 7321 gcaagcaatg cggacaaaat ggctctgact gcaaaaaaca atgccataga aggcaataaa 7381 aaaatgagtg aaatgctcaa agcgatgcag gacataaatg atgcatcaaa taatatatca 7441 agaataatca aggttattga cgacattgca tttcagacca acatacttgc acttaatgcc 7501 gccgttgagg ctgcaagggc gggacagtac gggaaaggat ttgccgttgt tgcagaagaa 7561 gtaagaaatc ttgctgcaag aagcgcaaat gcggcaaaag agactacgca gcttatagag 7621 ggttccatcc atacggttga ggctggtatg gaaatagcaa cggatacagc gaatgcgtta 7681 aatactattg ttgaagaaat ctcaaaagcg gcttcactgg tgcaggatat atctgtagct 7741 tcaaatgagc aggctaccgg cattgcacag ataaatcagg gtgtttccca ggtttcccag 7801 gttatccaga ctaattcggc cacggctgaa caaagtgctg ctgcaagtga ggaattgtca 7861 agccaggctg aagtattaaa agaaatggta aaaaagttca gacttaaacc gaacacaagt 7921 ctgccgggat atggggaaac agaccctgag gttataaagt atatggaaga tgtaatggag 7981 aaaaaggaga acaataagaa aggtaaaaaa actttacgaa cggattttaa catagatctt 8041 gacaatgaag attttgggaa atattaaaaa acaaagactg taaaaaaaga attttataca 8101 gttgacataa aacacatccc ggcgtagaat tggttgtatg tgaaataatt ttctgatggg 8161 gaatagaaaa tgcaaaaaga aacggcgtca ataaaaaaga tgagtgtctt tgctctcaca 8221 tggccgatat ttattgaaac gctgctgaga acaatgctgg gaaatgtgga tacttttatg 8281 ttgagtacat attccgacga tgccgtgggg gcagtagggg ttgtaagtca aataagttat 8341 atacttatca tgctgtacaa cgttgtttcg tcaggaacac tggtgcttat atcccagtat 8401 ctgggcgcaa aaaagaaaaa ggaagcttca gtggtggctg tcacttcaat tgcaggcagt 8461 ttgatatttg gtttgtttgt cggattggct gtatttctgt tcagaagcca gatattaaca 8521 tttcttaatt tgccgcccga acttatggga tatgctatga catttttggg aattgtcgga 8581 ggattttctt ttacccaggc attgatagcc actttgtctg caataatcag aagctatggc 8641 aacaccagga taaccatgta catttctgtc ggcatgaata tccttaatat tattggaaac 8701 agtattttcc tgtatggact gctgggggcg ccgaaaatgg gagtgaccgg tgttgccatt 8761 gcaactgtaa taagccaggc tgtcggtgtt gttgctatgc tgattgtaat gctgacagga 8821 cttaatcaaa aattttcttt ccgggacctt gtgccgctgc cgtgggagat tttaagggat 8881 atattgaaaa tcggacttcc ttctgcgggt gaaggaattg cttacgaagc atctcagctt 8941 accattaccc gtattataac ggtattggga aaggttgccc ttacaaccag ggtatacact 9001 ttaaacatta tgtattttgt aatggttttt tcagtagcgg ttggtcaggg aactcaaatt 9061 gttgtaggcc atcttgtggg ggcgggcgat aatgaaaaag catacaaaac atgtattaaa 9121 agtctgagat atgctgttgt ggtggcaatc attcttgcgg gaattgtttc gttcttttcg 9181 gagcagcttc ttggaatctt tacggatgac cgggctataa ttgaaatggg gagcaaactc 9241 ttgctgattg cagttatttt ggagccggga agagttttca atattgttat aataaactct 9301 ctgagagcgg cgggtgatgc cagatttccc gttattatgg gtattatatc catgtgggga 9361 ataggagtgt tgctgtcata tttcctgggt gtggcctgcg gcttgggatt gataggtgta 9421 tggatagcct ttgccagtga tgaatggttc agagggattg ccatgcttct gcgctggaga 9481 tcccgcgtct ggtataaaat ggcacttgta aaaaatcaga atattgaaat gccggcttag 9541 atctaacctg tgtcttgttg aaaagaattg caaaaaagct gtgaaaggaa tcaggtttct 9601 tttcacagct tttgtatttg gtgttttccc gggaaatatt tattttttta taaacatttg 9661 agtccagtaa agccttccgt ttggcccttt tgcaaggccg actccaattt ctgtgaaaga 9721 aggacttaat atattgtttc tatgaccggg agaattcatc caggcattca taacatctct 9781 ggctgtttgc tgtcccatgg caatgttttc gccagcggca gtaaatttta ttccaaagtc 9841 ttccatcatt ttaaaaggag aaccgtaagt gggggaagtg tgggaaaaat agcctttgtt 9901 agccatgtcc tcggatttgt atcttgccac tcttgaaagc tgccaatttg cctttaacgc 9961 cggaagccct ttttttgccc tttccacatt tacaagcctt ataacttcat tttcctgcgc 10021 ctttatatca tcaatgttcg gaatattaat tttctgtccg ggataaatga ggttagggtt 10081 tttaatttgc ggatttgctg caattatttc gctgatgcct atttgatatt ttacagcaat 10141 tttccacata gtgtctcccg gcttgacggt gtaaacctgt gtttgtgcaa aaacagtgct 10201 tgttgtaaat attaatatta acaacaaaag caataccaat atgttttttc ttttcataaa 10261 aacatcacct catgtaatat ttttttaata ttaccgggtt tttatgtttt aaatattatg 10321 taaataatac caggtttacc ttgaaataaa gcaaataata taaatatagt gttgtaatat 10381 tgaagtcgaa ttcttttagg tatatggaaa atataggaaa caatgcgggt gaacggaatg 10441 aaaacaaacc aggttattga ggtggcttta aaagcgggag aaatattgct taccggggga 10501 gcggaaatat acagggttga ggatacggtg acaagaatat gcggaagcta taatgtccgg 10561 gccgagtgtt ttgtgcttcc gacgggaata ttcataactg taatcgacag tgaaggaaat 10621 cccgtgtcca ttatccgacg aatcagaaag aggaccgtaa atttggaaca ggtggaaaaa 10681 gtaaattctt tttcaagaag ccttaaagat cgtactcttt cgtatcagga ggccatggca 10741 ggcttaaaag atattgcagc tttggatgga tatggctatg tgttaaaact tattgtggca 10801 ggaattgctc cttttgtata tgccctgctt tttagaggaa cggtcctgga agcatgtgtg 10861 tcttttttaa taggcattgt tatatatgcc ataaaagaaa aaatcaccca ggccggggtg 10921 tttgagtttt ttgagtattt tgcatccgga gtggccgccg gtgctttgag taccctggcg 10981 ataaagctgt ttccgtttat ggatttatac agaattatta ttgcgtccat tattatcctg 11041 cttccgggtc ttgccataac caatgcaatg aaggatgctc tttacggaga tattgtgtca 11101 agcatgttca gactcaccga ggcagttttt gttgccgctg cggtgggagt aggggtggca 11161 atagcactgt ctgtgggttt gcgttggatt taattgaggg agatggtgtg ttatggtgga 11221 aatttttctg gcgtttttag gcagtatttg tcccggggta atatacaatg ttgaaaaaag 11281 aaatttaatg tgggttggtt tcggcggtat gctggggtgg attacctacc gcacatttaa 11341 tgaaattacg aagagtcttg tacttgcatc ttttttggga gctgtgattg taggtattta 11401 cagtgaaagt gttgcaagac tcttaaaggc tcctgctacg atattttcgg tgcccggaat 11461 aattcccctg gtgccgggaa tagctgcata cagtactata cagcatgttg ccgagaatca 11521 gttgtatgaa gctgcgagca aaggaattga aacgatgtcc ggtgccgggg caattgcatt 11581 tggaatcttg cttgcctcgg cagttttcag actgacaaaa aagttgaaaa aaagaggacc 11641 gaatccatct taaagtaatt attgactggc agaattcggt atatttaaag tctttgttct 11701 atctatttcg gtaaaacagc aagagaaata caaggataaa aaacagtatt accgctgctt 11761 cattaaacgg actgaaacag ctggatctgt ctattgtgga agcttggaga gcttcggatg 11821 tgtctcgcgc ggtttccttt tcttccggag ctttaccggt atccaatgtt tccagtttgt 11881 tttcttcggt cactttatta cctccttttg aaccgctcaa aagtgataac gtcttatata 11941 taatataata ttcattggat accgtattgt tacactattt tattccatca gacaattgat 12001 ttctttgtgg acttcagagg taagtttcat taaattgcgc aatatcagcc tggattccgg 12061 attgtcgttt tttattttgg attttgccac tgcgcaaaaa aagtaaatca tattgaggct 12121 ttcggttaat aaaaacaggg cttcgtactt tgtaaaatac tcagaaccga tgtcctcatt 12181 aatggacaat ttcacaaaat cataatcatc aatcaaatct gcaagatact tttgcattga 12241 gtaaatattt tttcttgtgt ttcccgccag atattgtttg gcgcgcttat agcttttttg 12301 aagatttggg agcagaaagt tgtaaatgta atttcccata tccttaagat gaagcatttc 12361 cagttcgtat attgagtcca ttcctgcaaa gtcacaatta agacttagat tgctctgacc 12421 gtgcaattat atcactcctt ttgatttgat tgtactttga agatatttca agctattaca 12481 aaatattata ttggaatttg aacaaatatg accttggaaa tctgatggat aaaaaagaaa 12541 atgtgttaaa tgaagtgtga ataataaaat tagtcaacaa tataattata ccgcataatt 12601 tagcaaaaat caacaaaaaa attgaaatta tatttttcca tggtatttag cctttttagg 12661 tgttttataa gaaggaatgt ttctaaaaag ggcgaatatt gacacgaaca taagttttgt 12721 tgtatgctga atcataaggc acatcggtaa ttacaaaaat aatgggagtg acagagaggc 12781 tatgtcaaaa gaaaagcaga ataaggctga aagatatgga aacaccagta taaaatctct 12841 taagggtgca gaaagagtga gactgcgtcc ggcggttatc tttggttctg acggtattga 12901 aggctgccag cattcgtttt ttgaaatact ctccaactcg atagacgagg caagggaagg 12961 ttttggcaag gtgattgaag ttaccaggtt caaagaccgt tctatcatgg taaaagacta 13021 tgggcgggga ataccgctgg attataatga aaatgaaaag cgttacaact gggaactggt 13081 attttgtgaa ctctatgcag gtggaaagta caagaacaat tccggagaaa actatgaatt 13141 cagccttggg ctgaacggtc tgggaagctg tgcaacccag tacagctctg agtatatgga 13201 tgtcacggtt ttcagagacg gcatgaggta tgatttgcat tttgaaaaag gtgaaaatgt 13261 tggcggcctg aaaaagcaga aatgggacta tgaaagcacg ggaaccatta ttaaatggag 13321 accggatttg gaagttttta cggatataaa catacctctt gaatattata ccgaagtgct 13381 gagaaagcag gctgttgtca atgccggttt gaaatttata ttatatgacg aagaatcgga 13441 ccgggttttt gaatactgct acgaaaacgg aattgtggat tatataaaag aggtttcggg 13501 ggataaagca tttacggata ttcagtatta tgagacaagt acaagaggaa gagacaggga 13561 agacaaaccg gagtacaagt taaagatgca aatagctttt tgctttaaca acgaggtaaa 13621 cctccttgag tattatcaca actccagttt tttggagtat gggggtgctc ccgacaaagc 13681 ggtgaagaat gcgtttgtct atgttattga caaatttctc aaatcccagg gcaaatacaa 13741 caaggatgag tcaagaatta cgtttcagga tatacaggac agtcttatac ttgtatcaaa 13801 ctctttttcc accattacaa gttatgagaa ccaaacaaag aaatcaatta ccaataaatt 13861 catccaggag gcaatgaccg agtttctgaa agagaaactt gaggtttatt ttatagaaaa 13921 taaagttgaa tgtactaaaa ttttggaaca agttctggtg aacaaaagaa gcagggaaac 13981 cgcggaaaaa accagggtca atataaagaa gaagctcagc ggaaatgtcg atatttcaaa 14041 cagggtaaag aagtttgtgg actgcaggag caaggatgtt aacaaaagag agatttatat 14101 agtggaagga gactccgctt tgggctcctg taaacttgct cgggacgctg agtttcaggc 14161 tataataccg gtcaggggta aaattttaaa ctgccttaag gcggactatg acagtatttt 14221 caagaacgaa attattgttg acctcttgaa agtgctgggg tgcggtgttg agatcaagtc 14281 caagcacaac agagagctca gtacctttga tttgaacaat ctcaggtgga gcaaaatcat 14341 tatatgtacc gatgccgatg tggacggtta tcagataagg actcttatat tggctatgct 14401 ttacaggctg gttccgactt tgatacaggt tgggaaggtg tttattgcgg aatcaccgct 14461 gtttgaaata aggacaaagg ataaaaccta ttttgcttat accgacaagg aaaagaatga 14521 aatcgtttca aagctcaagg ggaaatatac catactgcgt tcaaaaggac ttggcgaaaa 14581 tgatcccgat atgatgtggc agaccaccat gaatccggaa acgagaaggc ttatacaggt 14641 ggttcctgac gatataaaga aaacagagga gttttttgac cttttccttg gggataacct 14701 tcccggaaga aaatcctata tcgaggaaca tggacacaaa tatcttgaca tgattgacgt 14761 gatgtaagga aatattaagt actaaaaatg tgaacagtcc agtaaaataa aaatgtgacg 14821 gtggaaaggt gaagtagtga tatgtcgaca aacaatttaa ttgaacagag aatagttgac 14881 actcttgaaa aaaactatat gccctatgcg atgagcgtaa ttgtttcgag ggcaataccg 14941 gagatagacg ggcttaagcc gtcccacaga aagctgcttt atacaatgta taaaatgggg 15001 ttgctcaccg gcgaaaaaac caagtcggca aatgtggttg gccaaaccat gaaacttaat 15061 cctcatggcg atatggcaat ttatgagact cttgtgaggc tgactcgcgg caacggtgct 15121 ctgttgcttc cgtacgtgga ttcgaaaggc aactttggaa agcattactc aagagacatg 15181 aaatatgctg caccgcgtta tactgaagtg aagcttgaca aaatatgtga agagcttttt 15241 aaagatattg acaaggatac ggtggacttt gttgacaact acgatggaac gatgaaggag 15301 ccaaagcttc ttcccgccac ttttcccacc attttggtaa atgccaacca tggaattgcg 15361 gtgggaatgg caagcagtat atgcagtttt aaccttgtag aggtatgcaa tgccgctgtc 15421 gagtttataa aagatgagaa cacggatctt gtcggaatcc taaaagcccc tgattttccc 15481 acgggaggac agcttatata taatgaacgg gaaatgaggg agatttacga aaaaggcaga 15541 ggaactttca aacttcgggc aaaatacagg tatgacaaag cgaacagctg catagaaata 15601 tatgaaatac cttatactac cacggttgaa gctattatag actctattgc ggaccttgta 15661 aaaagcaata aaattcggga catatccgat gtcagggacg agacggatct gaacggcctt 15721 aagatagcac tggatataaa aagaaatgca gaccctgaca ctctcatgaa caaactttac 15781 aaactcactc ctcttgagga cagtttcagc tgcaacttca acatactgat taacggtcgt 15841 cccatggtga tgggagtaag aggtattctt aaagaatggg ttaatttcag aatggactgc 15901 atcaagaggc agacccttta tgatatagac aagaaaaccc aaaaacttca tattcttctt 15961 ggcttggaga agattttact ggacattgac aaagccatta aaattataag gaacactgag 16021 gaagaagcaa tggtggttcc gaaccttatg gaaggttttg gaatagatca ggctcaggct 16081 gagtttgtgg ctgagataaa gcttagaaac ctcaacaaag aatatatttt aaaaagagtg 16141 agcgagattg aaagtttgaa aaaggaaatt gcggagctta atgaattata cggcagcgag 16201 aagaaaataa aaaagcttat cataaagcaa ctggaggaga ttgcgaagaa atacggacag 16261 cccagacgta ctgagattgt tatggaggag catattgaag agataactca tgaacatttg 16321 atagaggact acaatttgaa gctgttcctc acaaaacaga actatttgaa gaagattcct 16381 ctgacttcat tgagaaccag ccccgaacac aagctcaaag agggagactt tatagtgcag 16441 gagctggaga ctcacaacaa agcggatctt ctgttgtttt caaacaggca aaatgtatat 16501 aaaatgaaga tatatgaaat tgaagactgt aaagcaagca gccttgggga atttctttca 16561 aatgttttaa gccttgaaga gggtgaagag attattcata tggttgcgac ggacgactac 16621 aaaggatata tgatatttgg ctttgagaac ggcaaggtgg caaagatagt gcttgaaagc 16681 tatgcaacca aaaccaacag aaagaagctt gccaatgcat ataacggttt gtcaaggctg 16741 gtttatatcg gacatatttt ggaggatgaa gaactggttg cctttagcag tttgaacaaa 16801 gttcttattt tcaatacttc aaatataaat ccaaaaacaa cgagagattc ccagggagtt 16861 caggttttaa aatcgaaaaa aggcagtgta atgacccata taaagaaaat cagcgaggtt 16921 tcctttacag accctgacta ttacaggaca aagaatattc ctgccatagg ttgctatctt 16981 aaagatgaag atgttgaaga caaccaaata aaacttgaac tgtaaaaaag gtcaaacagg 17041 aaaaattatt attgctttta tgaaacggtt ttggtaatat ataaaacgta atacagcttt 17101 ggttgaataa tttcaaaagc tgtattatta tagaaaagtt tggtgaaaaa ccgaacttat 17161 tgtcagaaaa cataataatt tttgaaagga gtgggtcaaa tgtataaagg gcaagtgagg 17221 tcaccggcat tggttataat cctttcaata gtaacctgcg gtatttatgc ttggtactgg 17281 atttataaaa tgtcggagga attgcagaga gctactggaa agtcaacaat aagtcccggc 17341 gtggaactct tgttgtgtat tgtaacttgt ggtatttatc cgatttattg gttctataaa 17401 tatggaaaaa tgataactga agcccaggaa caggcaggat tgaatgctga agacaatgct 17461 gtgttgtatt tgttgcttgc tatttttggc ttgggtgttg taaacatggc tcttatgcaa 17521 tcggctgtta ataaaatatg ggagcaggat ataatataag aaatgggatt gtgccttttt 17581 aagaattttt ttggcattcc ctgcccggga tgtggaatga cgagggcata tttgagtttg 17641 attaaattcg atttgacatc ggctttttat tatcatcctc tgtttctact tcccggttta 17701 gtagctgtgg ttgttgtttt cagaaacaac agatttttgg gacgcctgta cagaaataat 17761 attttatggt gttgtgtgct ggcggtattt gttcttgtgt ggattgtaag gctagtactg 17821 ttttttcctg acattccgcc gatgaattac aattcacagg ctttgatacc gcggcttttg 17881 ggattgttta aaatcgaata ataaaatttt gagaacaaaa aacaaagaaa tttgagagca 17941 aaggaaccac aaaatatttt tgtggtttct tttacttttt ccaaacatat gtttgaatac 18001 atatgcaact gtggtataat aaaatgaagc aaccttaccg gcagaataga ggtgcatgtt 18061 tttatgcata aatttaaact ggtgtctgat tacaaaccct gcggagatca accggaggct 18121 atagataaac ttgtggaagg cattaacagg ggatacaggg gtcagactct tttgggtgtg 18181 accggttccg gtaaaacatt taccatggcc aatgttattg aaagggttca gaaacccacc 18241 cttgtgattg cccacaacaa gactcttgcg gcacagcttt gcagtgagtt taaggagttt 18301 tttcccaaca actgtgttga atattttgta agctattatg actattacca gccggaggct 18361 tatattcccg ccaccgatac ctatattgag aaggattcgt cgataaatga cgagattgac 18421 aagctgaggc actcggctac tgctgcgctg tttgaacgaa gggacgttat aatagttgca 18481 agcgtttcgt gtatttacgg tttgggagac ccggaagatt atactgattt gatgctttcg 18541 ttgcgtcccg gcatgataaa ggacagggat gaaataatca ggaagctggt ggacattcag 18601 tacgaaagaa atgaaattga ttttaaaaga ggaaaattca gggtcagggg tgacatactg 18661 gagatattcc cggcttcatc ttctgacaaa gtgatacggg tggagttttt cggagaagaa 18721 attgatagga ttaccgaagt tgattcactg accggtgaaa taaccggagt atgctctcat 18781 gttgccattt ttccggcttc ccactatgcc acgacaaagg ctaaaatgca aagggccata 18841 gcttcaattg aacaggagct tgaggagcgt gtcagggaat taaaatccca gggaaaactc 18901 cttgaagccc agaggcttga acagcggaca aggtatgacc ttgaaatgat gcaggaaata 18961 gggttttgcc aaggtataga gaattattcg agacatataa gcggaagagc tccgggcagt 19021 ccgcctttta cgcttattga ttacttcccg aaggattttt tgctgataat tgacgagtcc 19081 catgtgacca ttccccagat tggagccatg tacaacggtg accggtcgag aaaggaatcc 19141 cttgtggaat acggtttcag gctcccttcg gcttttgaca acagaccgct tacgtttgag 19201 gagtttgaga aaaaaatcaa ccaggttata tttgtcagtg ctacgccggc aaagtatgaa 19261 agggagcatt cgcagcagat agtggagcag attataaggc ctacagggtt gcttgatcct 19321 gagattgttg tgaaacctgt aaaaggtcag attgacgacc tgattgggga aataagtgaa 19381 agagtacaga aaaatcagag agttatgatt accaccctta ccaaaaagat ggcggaggat 19441 ttgacggatt atttgaggga gcttgacttt aaagttgaat atttgcattc tgacattgat 19501 accattgaga gaatggaaat aatacgaaac ttaagacttg gagtttttga cgtcctggtt 19561 ggcataaatt tgctaagaga aggtctggat attccggaag tgtcccttgt ggccattctg 19621 gatgccgaca aggaaggctt cctgcgttcg gagacctcat tgattcagac tataggaaga 19681 gcggccagaa atgttgaggg aaaagtcata atgtatgccg ataccattac ggactcgatg 19741 agaagagcca ttgacgagac caacagaaga agaaagatac agtcggaata caatcagaag 19801 cacggtataa cgccgaagag cgttcaaaag ggtataaggg atgtaattga aattacaaaa 19861 gttgctgaag aagatgcaaa gtactttatt cgcggcgacg aggattcgat ggataaggat 19921 gaagttttgg accttattga aaaactcacc aatgaaatga aggcggcagc ggcggaactt 19981 caatttgaac gggctgcgga gctcagggac aaaattgccg aactgaaaaa gaaaatagga 20041 gcttaaagaa aaaacaaaat gttagtttca attagtccag cacgtatacc actacttcct 20101 gcaatccaaa tttcatggct ttgttgtata ttaccgtttc gcccggtttg tcttcgccaa 20161 agaaaatatc tattttattg cccttgatta ggcttccggt atcttcggct atgtatattc 20221 cgtcaagatg gctgtatgcc acgggaaaag atatgtacac cctggttccg agaggaatga 20281 cggacggatc cacggcaacc gttcttccga ctgtggcgcg tgttccggag gctgtaattc 20341 catacgcagg atgttcacgg gttttgccgc agcttttata tgagaggtca tatgccgtag 20401 ccgtcatatt gattatcctt ttgcactggg gcatgatgtc cgcaatttcc acctttgtgc 20461 gtttgacttg ggtttgggca gactttgcct cagttgcaga atttgatttg gcggtcagtt 20521 tcgacctcga aacaggtgtg ctttgggttt ttgaagtatt tttgctttct atgctttttt 20581 taatttcagc aaaggaaatg ttcgcggtat tcacagctga aacagaagcc agggctcctt 20641 cggtttttgc cggcatactt tctttgacga ggcttgacaa ttttgcactg tctggggata 20701 cccaggtgga acttgcgaaa ttgtgcacat ggctggctgt tttttctgtg attgaattga 20761 gaatgttgtt accggcgcag gtaagcagga aggtcatcaa tgatcctgca gccacgactt 20821 tcaaatccaa atccttttta ctaagaattg attggctgca gcatttgacc cgctccaaaa 20881 tggaaggtga tttggaggat aaaacggctg aatcaacttt ccttattatt tcttcaagta 20941 tttccggata gtttaatttc agttgtgctg cattcatgag aacttcttcg agaatttcgg 21001 gataattcag actgttgatt tcctcttcct ggaaatactc agacaggtat tcccggctgc 21061 ggctatgaac tctgttgaga tccgggcagt taaaaatatt acctattgta tttcccatct 21121 taatcccccc atcttaagag cacttttccg caagcacctt gtctatgagc ctctgttttg 21181 ttatctctat attctattaa aaaagaggtt tggcatattc ataaaaatca tgtaaattcc 21241 ataaacaaca ttgacggaat gctgatatat cggcaaatac gcaggataaa aatacaaatt 21301 catatttcaa taattatatt ataatacaaa atggaatcct ttcctagtac aaaacataaa 21361 aaatctggaa attaatttac atttgttcac aaaagcgtct tggttgcata attgtaagtg 21421 tgttgacatg gctgtttaca tctcttcaaa aatggataaa tatttattaa aaaagttcga 21481 acaaatgttt gaaaacggaa atatatttga tataataata gctgtttata aaaaaagtga 21541 tggtgaaatt ccaattaaat ttaaaataga gcaggtgttt gtatgaaaaa ggattatatt 21601 gttgtaaagg gtgccagaga gcacaatttg aaaaacatag atgtcaagat tcccagggac 21661 aagtttgttg ttatcaccgg actgagcgga tcaggcaaat catcccttgc ttttgacaca 21721 atatatgcgg agggacaaag gcgctacgtt gagtcattgt cctcgtatgc aagacagttt 21781 ttaggacaaa tggaaaaacc tgatgtcgat tatattgacg gactgtcgcc ggccatagcg 21841 atagatcaga aaaccacaag ccgcaatccc cgttccactg tgggcacggt tacggagata 21901 tatgattatt taaggcttct ttttgcaaga ataggcactc cccactgcta cttatgcgga 21961 agggaaattt cccagcaaac ggtggaccag atggtggaca gaattatgga gtttgaagaa 22021 ggcacacgga ttcagcttct tgctcctgtg gtaagaggaa gaaaaggtga gtatcacaag 22081 ctcatagaag atataaagaa ggaaggctat gtcaggatta gagtggatgg agaggtagtg 22141 gatgtaaatg accctgtaaa cctcgacaag aacaagaagc acaatattga aattgtggtg 22201 gacaggctga ttgtgagacc gggaattcag aaaaggttga cagattccat tgagactgtt 22261 ctgcgcttaa gcaacggcat acttgtggtt gatgtcatag gcggaaagga gatgctccta 22321 agccaaaact ttgcatgtac cgaatgtaac gtgagcatgg aggaaataac gcccagaatg 22381 ttttctttca acaatcctta cggtgcctgt cccgaatgta ctggtctggg ctctcttatg 22441 aggatagacc ctgaccttgt cataccggac aaaaaacttt ctttggccca gggagccgtc 22501 agggcgtcag gatggaatat agcaaatgat gaaagctatg ccagaatgta tatagacgct 22561 cttgcaaaac attataattt cagcgtggat acccctgttg aggagcttcc cccgcatatt 22621 cttgacatta tactctatgg caccaacggg gaaaaaatta aaatagaata tgaaagggaa 22681 aatgaaaaag gaacattcat ggcaagcttc ccgggaatta taaacagtat ggagagaaga 22741 tacaaagaga caacttcgga agtaatgaag cagtactatg aaaactttat gagcaatata 22801 ccctgtcctg tctgcaaggg ggcgagattg aaaaaggaaa gtcttgcagt gacaataggc 22861 ggcaaaaata tatatgaagt ttgctgcttg tccattggag aagcaaaaga gtttttcgca 22921 aatttaaacc ttacggaaag gcagcagctt attgcccgcc agatattgaa ggaaataaat 22981 gcaagactgg gatttttggt ggatgtgggg cttgactacc tcacccttgc gagagcggca 23041 ggaacactgt ccggaggtga agcccagaga atcaggcttg ccacacaaat tggctcggga 23101 cttatgggag ttatatatat cctggacgag cccagcatag gtcttcatca gagggataac 23161 gacaggctcc tcagaagtct caagaagcta agggatttgg gaaatacttt gctggtggtt 23221 gaacatgatg aggatacaat gtatgcgtcg gattacatta ttgatttggg accgggtgcg 23281 ggaagccacg gaggacaaat agttgcggaa ggtactgtgg aagagattaa acaaaatccc 23341 aattccgtta cgggagagta tcttagcggc agaaagaaaa ttgaagttcc taaagaaaga 23401 agaaaaccca atgggaaatg gctggaaatt ataggagcaa gagaaaataa tcttaaaaat 23461 ataaatgtaa gaataccttt aggagtgttt acgtgcatta caggggtttc aggatccggg 23521 aagagttctc tgataaatga aattttgtac aagcgattgg ccgccgagct taacagagca 23581 agtgtaaaac cgggcgagca tgacttgata aaaggaattg agtatcttga caaagttata 23641 gatatcgacc agtcgcccat tggccgcacg ccaaggtcca accctgcaac atacacaggt 23701 gtgtttgatt ttataaggga aatatttgca aacaccactg aagcaaaaac ccgggggtac 23761 aaggcgggac gtttcagttt taatgtaaag ggcggcagat gcgaagcctg tgccggtgac 23821 ggtataaaca aaattgaaat gcacttttta ccggacattt atgttccctg tgaggtttgc 23881 aagggcaagc gctacaacag agagaccctt gaagtaagat acaaaggaaa aaatatagcg 23941 gaagttctgg atatgactgt ggaagaggca ttggagttct ttaagaatat accaaggata 24001 cacaaaaaga tagaaacatt gtatgatgtg ggtcttggct acatcaaact gggacagtcg 24061 tccaccactc tgtccggagg cgaggctcag agggtaaaac ttgccaccga gctttcgaga 24121 aagagcactg ggaaaacaat atatatactg gatgagccga ctacaggcct tcatatggct 24181 gatgtgcaca ggcttgtcgg catacttcac agactggtgg aggcgggaaa ttctgtagtg 24241 gttattgaac ataaccttga cgtaataaaa actgccgatt atattattga tttgggacct 24301 gaaggtggca gcggaggagg tctcgttgtt gccgagggga caccggaaga agtggcaaag 24361 gttgaaaatt cttatacagg acagtttttg aaaaaagttt tgtccactta aatgaaggtt 24421 ttaacatttt tgtatttatg tttgattaag atgacgatat acttcttgaa aagcaggtat 24481 tgttatttat tgcaggttaa actcaatttt tagctgagtt gagttttatg ggcaatggaa 24541 tattgttaga ccaaatgcat aaatatgtgt tatacttgaa acgtgataat tttataattt 24601 tatatgcata aacctggata atccatattt taattttgag tattatacta tggaggttaa 24661 aatatatttt accaataatt t //