LOCUS NZ_ACVX01000052 13996 bp DNA linear CON 10-NOV-2010 DEFINITION Clostridium thermocellum DSM 2360 ctg00021, whole genome shotgun sequence. ACCESSION NZ_ACVX01000052 NZ_ACVX00000000 VERSION NZ_ACVX01000052.1 GI:256005968 KEYWORDS WGS. SOURCE Clostridium thermocellum DSM 2360 ORGANISM Clostridium thermocellum DSM 2360 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium. COMMENT PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from ACVX01000052. FEATURES Location/Qualifiers source 1..13996 /organism="Clostridium thermocellum DSM 2360" /mol_type="genomic DNA" /strain="DSM 2360" /isolation_source="contaminant of Clostridium thermocellum strain LQ8" /db_xref="taxon:572545" gene 212..403 /locus_tag="ClothDRAFT_2771" CDS 212..403 /locus_tag="ClothDRAFT_2771" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430910.1" /db_xref="GI:256005969" /translation="MDGYLKQYVGKKIVVYLSLWSMLFHVGVSGLVSDCRDGWIELKR KNRVEHINIGKIYCFRVIE" gene complement(487..1320) /locus_tag="ClothDRAFT_2772" CDS complement(487..1320) /locus_tag="ClothDRAFT_2772" /inference="protein motif:PFAM:PF00232" /note="PFAM: glycoside hydrolase family 1; KEGG: scl:sce4297 Beta-glucosidase" /codon_start=1 /transl_table=11 /product="glycoside hydrolase family 1" /protein_id="ZP_05430911.1" /db_xref="GI:256005970" /db_xref="InterPro:IPR001360" /translation="MLGHFLGIHAPGIKDLRTSLEVSHNLLLSHGKAVKLFREMNIDA QIGIALNLSYHYPASEKAEDIEAAELSFSLAGRWYLDPVLKGRYPENALKLYKKKGIE LSFPEDDLKLISQPIDFIAFNNYSSEFIKYDPSSESGFSPANSILEKFEKTDMGWIIY PEGLYDLLMLLDRDYGKPNIVISENGAAFKDEIGSNGKIEDTKRIQYLKDYLTQAHRA IQDGVNLKAYYLWSLLDNFEWAYGYNKRFGIVHVNFDTLERKIKDSGYWYKEVIKNNG F" misc_feature complement(517..>1317) /locus_tag="ClothDRAFT_2772" /note="Glycosyl hydrolase family 1; Region: Glyco_hydro_1; cl01046" /db_xref="CDD:186308" gene complement(1337..1903) /locus_tag="ClothDRAFT_2773" CDS complement(1337..1903) /locus_tag="ClothDRAFT_2773" /EC_number="3.2.1.21" /inference="protein motif:PRIAM:3.2.1.21" /note="PFAM: glycoside hydrolase family 1; KEGG: scl:sce3455 Beta-glucosidase" /codon_start=1 /transl_table=11 /product="Beta-glucosidase" /protein_id="ZP_05430912.1" /db_xref="GI:256005971" /db_xref="InterPro:IPR001360" /translation="MFPLGYNYIITLFANNILKGVVNMSKITFPKDFIWGSATAAYQI EGAYNEDGKGESIWDRFSHTPGNIADGHTGDVACDHYHRYEEDIKIMKEIGIKSYRFS ISWPRIFPEGTGKLNQKGLDFYKRLTNLLLENGIMPAITLYHWDLPQKLQDKGGWKNR DTTDYFTEYSEVIFKKSRRYRSNMVYSQ" misc_feature complement(<1376..1819) /locus_tag="ClothDRAFT_2773" /note="Glycosyl hydrolase family 1; Region: Glyco_hydro_1; cl01046" /db_xref="CDD:186308" gene 2113..2886 /locus_tag="ClothDRAFT_2774" CDS 2113..2886 /locus_tag="ClothDRAFT_2774" /inference="protein motif:PFAM:PF06161" /note="PFAM: protein of unknown function DUF975; KEGG: scl:sce8934 hypothetical protein" /codon_start=1 /transl_table=11 /product="protein of unknown function DUF975" /protein_id="ZP_05430913.1" /db_xref="GI:256005972" /db_xref="InterPro:IPR010380" /translation="MELGRLKRRARFQLSNNGANLALLYMMTMAFYILLFLLLGRFSL QTALIINGPVLLGFTIYFLAIARVEKEPRTRKEKEEKDKEKKLKGKFTVFGMEIKLGK KIKINLDTAEHPYRVSLKYVFYGFKYFINAAALYIWTTLLILVWSLLLIVPGIIKALD LSMSFFILADNPTMDIRKAADLSTKIMKGHRKELFLLTLSFIGWILISIFTFSVGFAI TIPYIMTTYALFYDEVKKQSIENGIIAEDEIGTRRLWEY" misc_feature <2569..2820 /locus_tag="ClothDRAFT_2774" /note="Protein of unknown function (DUF975); Region: DUF975; cl10504" /db_xref="CDD:158889" gene 3124..3264 /locus_tag="ClothDRAFT_2775" CDS 3124..3264 /locus_tag="ClothDRAFT_2775" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430914.1" /db_xref="GI:256005973" /translation="MALQAEPVSRKCRVAAVICKRVLACLEDMQEFGWYREANLRPFV LG" gene 3358..4377 /locus_tag="ClothDRAFT_2776" CDS 3358..4377 /locus_tag="ClothDRAFT_2776" /EC_number="6.1.1.20" /inference="protein motif:TFAM:TIGR00468" /note="KEGG: ppd:Ppro_2304 phenylalanyl-tRNA synthetase, alpha subunit; TIGRFAM: phenylalanyl-tRNA synthetase, alpha subunit; PFAM: phenylalanyl-tRNA synthetase class IIc; aminoacyl tRNA synthetase class II domain protein" /codon_start=1 /transl_table=11 /product="phenylalanyl-tRNA synthetase, alpha subunit" /protein_id="ZP_05430915.1" /db_xref="GI:256005974" /db_xref="InterPro:IPR002319" /db_xref="InterPro:IPR004188" /db_xref="InterPro:IPR004529" /db_xref="InterPro:IPR006195" /translation="MKEQLNSIRVQAEQELSNVGTIAELENIRVKYLGKKGELTAVLR GMGSLSPEERPVIGQLANEIRAYIESRIEEARNELIKKERSQKLEREVIDVTMPGKRK MLGKKHPLSIVIDEIKDVFIGMGYEIAEGPEVELDYYNFEALNIPRNHPARDVQDTFY INNNILLRTQTSPVQIRVMENKKPPIKIICPGRVYRSDAVDATHSPIFHQVEGLVVDK GVTMGDLVGTLRVFAKSLFGEKTEIRLRPHHFPFTEPSAEVDVSCWACGGTGCRICKN EGWIEILGAGMVHPKVLEVCGIDPEVYSGFAFGLGVERTAMGRFNIDDMRLLYENDIR FLKQF" misc_feature 3358..4374 /locus_tag="ClothDRAFT_2776" /note="phenylalanyl-tRNA synthetase subunit alpha; Validated; Region: pheS; PRK00488" /db_xref="CDD:179046" misc_feature 3406..3624 /locus_tag="ClothDRAFT_2776" /note="Aminoacyl tRNA synthetase class II, N-terminal domain; Region: Phe_tRNA-synt_N; pfam02912" /db_xref="CDD:111764" misc_feature 3679..4359 /locus_tag="ClothDRAFT_2776" /note="Phenylalanyl-tRNA synthetase (PheRS) alpha chain catalytic core domain. PheRS belongs to class II aminoacyl-tRNA synthetases (aaRS) based upon its structure and the presence of three characteristic sequence motifs. This domain is primarily responsible...; Region: PheRS_alpha_core; cd00496" /db_xref="CDD:29807" misc_feature order(3679..3681,3688..3690,3697..3702,3721..3723, 3736..3741,3745..3753,3802..3804,3817..3819,3829..3846, 3889..3891,3910..3918,3928..3930,3934..3936,3940..3942, 3946..3948,3970..3972,4021..4029,4033..4038,4096..4098, 4117..4125,4129..4131,4222..4227,4342..4344,4348..4356) /locus_tag="ClothDRAFT_2776" /note="dimer interface; other site" /db_xref="CDD:29807" misc_feature 3733..3753 /locus_tag="ClothDRAFT_2776" /note="motif 1; other site" /db_xref="CDD:29807" misc_feature order(3817..3825,3865..3867,3871..3873,3880..3882, 3943..3945,3949..3951,3964..3972,3979..3981,3985..3987, 3991..3993,4105..4107,4111..4116,4198..4215,4279..4290, 4297..4299,4330..4332) /locus_tag="ClothDRAFT_2776" /note="active site" /db_xref="CDD:29807" misc_feature 3940..3951 /locus_tag="ClothDRAFT_2776" /note="motif 2; other site" /db_xref="CDD:29807" misc_feature 4282..4299 /locus_tag="ClothDRAFT_2776" /note="motif 3; other site" /db_xref="CDD:29807" gene 4419..5654 /locus_tag="ClothDRAFT_2777" CDS 4419..5654 /locus_tag="ClothDRAFT_2777" /inference="protein motif:PFAM:PF03483" /note="PFAM: B3/4 domain protein; t-RNA-binding domain protein; KEGG: mxa:MXAN_3595 phenylalanyl-tRNA synthetase subunit beta" /codon_start=1 /transl_table=11 /product="B3/4 domain protein" /protein_id="ZP_05430916.1" /db_xref="GI:256005975" /db_xref="InterPro:IPR002547" /db_xref="InterPro:IPR005146" /translation="MKAPIDWLKDYVDINVSTKELADAMTMSGSKVEGIEVQGEEITK VVVGKILSLEKHPDADRLQVAKVDVGSEVLQIVTGAQNVNVGDYIPVALVGSTLPGGK KISKGKLRGVESYGMMCSIEELGLTRDDCPDAPEDGIYILPKEEELGKDIKEVLGLNK EIIEFEITSNRPDCLSIIGLAREAAVTLKTKFKRPVINVKEEGDDASQYISVEVKDTG LCPRFAARVVKDVKIGPSPKWMRDRLKAAGVRPINNIVDITNYVMLEYGQPMHAYDIK DIKGNKIIVRRAFDGEIIKTLDDQDRELDSSMLVIADEERAVGVAGVMGGANSEIKDD TKTIVFEAANFNGTSVRLTAKKLGMRTEASGRFEKGLDPENVEAAINRAAQLVEELGI GTVCKGMIDCYPEKKSQEL" misc_feature 4551..4874 /locus_tag="ClothDRAFT_2777" /note="tRNA-binding-domain-containing prokaryotic phenylalanly tRNA synthetase (PheRS) beta chain. PheRS aminoacylate phenylalanine transfer RNAs (tRNAphe). PheRSs belong structurally to class II aminoacyl tRNA synthetases (aaRSs) but, as they aminoacylate...; Region: tRNA_bind_bactPheRS; cd02796" /db_xref="CDD:48399" misc_feature order(4599..4601,4635..4637,4698..4700,4737..4739, 4758..4760,4767..4769) /locus_tag="ClothDRAFT_2777" /note="putative tRNA-binding site; other site" /db_xref="CDD:48399" misc_feature 4854..>5117 /locus_tag="ClothDRAFT_2777" /note="phenylalanyl-tRNA synthetase subunit beta; Reviewed; Region: pheT; PRK09616" /db_xref="CDD:181993" misc_feature 5073..5591 /locus_tag="ClothDRAFT_2777" /note="B3/4 domain; Region: B3_4; cl11458" /db_xref="CDD:164228" gene 5711..6805 /locus_tag="ClothDRAFT_2778" CDS 5711..6805 /locus_tag="ClothDRAFT_2778" /EC_number="6.1.1.20" /inference="protein motif:PRIAM:6.1.1.20" /note="KEGG: pca:Pcar_1423 phenylalanyl-tRNA synthetase, beta subunit" /codon_start=1 /transl_table=11 /product="Phenylalanine--tRNA ligase" /protein_id="ZP_05430917.1" /db_xref="GI:256005976" /db_xref="InterPro:IPR005121" /db_xref="InterPro:IPR005147" /translation="MIDILRALEFEVDENNMTVKVPTFRDDVEREADIAEEIARFYGY NNIEATLLSGKTATQGRKTYKQTIEDLIKETMIACGLCETYTFSFTSPKVFDKLNLPA DSELRKAVVISNPLGEDYSIMRTTTIPDMLAVISTNYNRRIEEARLFEMSKVYIPKSL PLEELPEEKTVLTLGMYGELDFYDLKGVVEELLERLGIKNYDVSPEKNNVVFHPGRTA LINIDGEYAGIIGEIHPEVAEKFECPERTYIGVIEVETLVRKASMDCQYKGLPKYPAV TRDIAMLVKDEVMVKEIEDIIKQRAGKILESVKLFDVYKGKQVPEGMKSVAYSITFRA SDRTLTDEEVGKAMTKILDGLKRNLGAELR" misc_feature 5900..6481 /locus_tag="ClothDRAFT_2778" /note="Phenylalanyl-tRNA synthetase (PheRS) beta chain core domain. PheRS belongs to class II aminoacyl-tRNA synthetases (aaRS) based upon its structure. While class II aaRSs generally aminoacylate the 3'-OH ribose of the appropriate tRNA, PheRS is an...; Region: PheRS_beta_core; cd00769" /db_xref="CDD:29814" misc_feature order(5906..5911,5930..5932,5939..5941,5951..5953, 5957..5959,5963..5971,6038..6049,6080..6082,6122..6124, 6152..6154,6167..6169,6173..6175,6179..6181,6200..6202, 6272..6274) /locus_tag="ClothDRAFT_2778" /note="dimer interface; other site" /db_xref="CDD:29814" misc_feature 5951..5971 /locus_tag="ClothDRAFT_2778" /note="motif 1; other site" /db_xref="CDD:29814" misc_feature 6098..6109 /locus_tag="ClothDRAFT_2778" /note="motif 3; other site" /db_xref="CDD:29814" misc_feature 6173..6181 /locus_tag="ClothDRAFT_2778" /note="motif 2; other site" /db_xref="CDD:29814" misc_feature 6521..6802 /locus_tag="ClothDRAFT_2778" /note="Ferredoxin-fold anticodon binding domain; Region: FDX-ACB; pfam03147" /db_xref="CDD:145994" gene complement(6876..7100) /locus_tag="ClothDRAFT_2779" CDS complement(6876..7100) /locus_tag="ClothDRAFT_2779" /inference="protein motif:PFAM:PF00269" /note="PFAM: small acid-soluble spore protein alpha/beta type" /codon_start=1 /transl_table=11 /product="small acid-soluble spore protein alpha/beta type" /protein_id="ZP_05430918.1" /db_xref="GI:256005977" /db_xref="InterPro:IPR001448" /translation="MARRNTSLDPKAREALNRFKIECAKEIGYLQFVKENNDHYKGDV PCRINGLQGGPIGGQMVKRMIEMAKSELIK" misc_feature complement(6882..7088) /locus_tag="ClothDRAFT_2779" /note="Small, acid-soluble spore proteins, alpha/beta type; Region: SASP; pfam00269" /db_xref="CDD:144014" gene 7451..8797 /locus_tag="ClothDRAFT_2780" CDS 7451..8797 /locus_tag="ClothDRAFT_2780" /EC_number="5.3.1.9" /inference="protein motif:PRIAM:5.3.1.9" /note="PFAM: phosphoglucose isomerase (PGI); KEGG: afw:Anae109_2086 glucose-6-phosphate isomerase" /codon_start=1 /transl_table=11 /product="Glucose-6-phosphate isomerase" /protein_id="ZP_05430919.1" /db_xref="GI:256005978" /db_xref="InterPro:IPR001672" /translation="MERIKFDYSKALPFVSEREVAYFENFVRSAHDMLHNKTGAGNDF VGWVDLPVNYDREEFARIKAAAEKIKSDSDALVVIGIGGSYLGARAAIEMLSHSFHNL MPKSKRNAPEIYFVGNNISSTYIADLLEVIEGKEISVNVISKSGTTTEPAIAFRIFKE YMENKYGKDGASKRIYATTDKEKGALRKLATEEGYETFVVPDDIGGRFSVLTAVGLLP IAVAGIDIDSMMKGAADARELYSNPNLMENDCYKYAAVRNALYRKNKTIEIMVNYEPS LHYFTEWWKQLYGESEGKDQKGIFPAGVDFTTDLHSMGQYIQDGLRNIFETVIRVEKP RKNIVIKEEKDNLDGLNFIAGKDVDYVNKKAMEGTVLAHTDGGVPNLVVTVPELSAYY FGNMVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEEQRKKLEERL" misc_feature 7451..8794 /locus_tag="ClothDRAFT_2780" /note="glucose-6-phosphate isomerase; Provisional; Region: pgi; PRK14097" /db_xref="CDD:184504" misc_feature 7616..8116 /locus_tag="ClothDRAFT_2780" /note="Phosphoglucose isomerase (PGI) contains two SIS (Sugar ISomerase) domains. This classification is based on the alignment of the first SIS domain. PGI is a multifunctional enzyme which as an intracellular dimer catalyzes the reversible isomerization of...; Region: SIS_PGI_1; cd05015" /db_xref="CDD:88410" misc_feature order(7691..7693,7697..7702,7877..7885,7892..7894, 8066..8068) /locus_tag="ClothDRAFT_2780" /note="active site" /db_xref="CDD:88410" misc_feature order(7802..7813,7898..7900,7910..7912,7919..7921) /locus_tag="ClothDRAFT_2780" /note="dimer interface; other site" /db_xref="CDD:88410" misc_feature 8249..8743 /locus_tag="ClothDRAFT_2780" /note="Phosphoglucose isomerase (PGI) contains two SIS (Sugar ISomerase) domains. This classification is based on the alignment of the second SIS domain. PGI is a multifunctional enzyme which as an intracellular dimer catalyzes the reversible isomerization of...; Region: SIS_PGI_2; cd05016" /db_xref="CDD:88411" misc_feature order(8270..8272,8306..8308,8318..8320,8327..8332, 8360..8362,8366..8374,8381..8386,8393..8398,8405..8407, 8522..8524,8528..8530,8552..8554,8561..8563) /locus_tag="ClothDRAFT_2780" /note="dimer interface; other site" /db_xref="CDD:88411" misc_feature order(8306..8308,8318..8320) /locus_tag="ClothDRAFT_2780" /note="active site" /db_xref="CDD:88411" gene complement(8937..9743) /locus_tag="ClothDRAFT_2781" CDS complement(8937..9743) /locus_tag="ClothDRAFT_2781" /inference="protein motif:PFAM:PF00149" /note="PFAM: metallophosphoesterase; KEGG: pca:Pcar_0763 phosphohydrolases" /codon_start=1 /transl_table=11 /product="metallophosphoesterase" /protein_id="ZP_05430920.1" /db_xref="GI:256005979" /db_xref="InterPro:IPR004843" /translation="MNILYFVLLLIILALVYMYYETTRLKISRVCFTESKEPFKIVHM SDLHIKFLNVDINKVVKVLETEKPDLVVLTGDYIDNPKHIPAFIKFLESIKGNYKICL CFGNHDYKALKNSEDSIQEFKQLIEEKGVSVLLNSSVCIEKGNRKYNVIGIEDLRSKR YDVKKALAGCNTKGCTNIAISHNPDIILQLPGGSVDYLLCGHFHGGQIWMPFNLEFRI LRHEKLTKIGITKGTHKLNNINLHINSGLGNVCVPLRLFSPPEISILHLP" misc_feature complement(8943..9632) /locus_tag="ClothDRAFT_2781" /note="Bacillus subtilis YkuE and related proteins, C-terminal metallophosphatase domain; Region: MPP_YkuE_C; cd07385" /db_xref="CDD:163628" misc_feature complement(9132..9629) /locus_tag="ClothDRAFT_2781" /note="Calcineurin-like phosphoesterase; Region: Metallophos; pfam00149" /db_xref="CDD:143917" misc_feature complement(order(9132..9134,9138..9140,9198..9200, 9423..9428,9516..9518,9600..9602,9606..9608)) /locus_tag="ClothDRAFT_2781" /note="putative active site; other site" /db_xref="CDD:163628" misc_feature complement(order(9132..9134,9138..9140,9198..9200, 9426..9428,9516..9518,9600..9602,9606..9608)) /locus_tag="ClothDRAFT_2781" /note="putative metal binding site; other site" /db_xref="CDD:163628" gene 9960..10124 /locus_tag="ClothDRAFT_2782" CDS 9960..10124 /locus_tag="ClothDRAFT_2782" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430921.1" /db_xref="GI:256005980" /translation="MFNFLNNDYELQPRTYSIKSYRRRESSIGKKMARLLVWLVILII LGVAAFLFFY" gene 10249..10332 /locus_tag="ClothDRAFT_R0047" /note="tRNA-Leu1" tRNA 10249..10332 /locus_tag="ClothDRAFT_R0047" /product="tRNA-Leu" gene complement(10601..11074) /locus_tag="ClothDRAFT_2783" CDS complement(10601..11074) /locus_tag="ClothDRAFT_2783" /inference="similar to AA sequence:KEGG:SeSA_A0856" /note="KEGG: sew:SeSA_A0856 putative cytoplasmic protein" /codon_start=1 /transl_table=11 /product="putative cytoplasmic protein" /protein_id="ZP_05430922.1" /db_xref="GI:256005981" /translation="MKLRRKDREVTEIKELIQIIDQCKVCRIAIQDSAGLYIVPMNFG YAYENNQLVLFFHSAKDGRKVRALKENSDVCFEMDCEHRLITGDTACQYSYSFKSIIG NGKVVFIDDAEEKKAALSALMKHQTGKDFSFDDKMIDSVLVFKIIVHEFTGKYHP" misc_feature complement(10613..11074) /locus_tag="ClothDRAFT_2783" /note="Pyridoxine 5'-phosphate (PNP) oxidase-like proteins; Region: PNPOx_like; cl00381" /db_xref="CDD:185954" gene 12023..12148 /locus_tag="ClothDRAFT_2784" CDS 12023..12148 /locus_tag="ClothDRAFT_2784" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430923.1" /db_xref="GI:256005982" /translation="MKRVIMIFAVFILVAIFNTFVYAGEEYQMIMNGAQSALALE" sig_peptide 12023..12094 /locus_tag="ClothDRAFT_2784" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.999 at residue 24" gene 12148..12492 /locus_tag="ClothDRAFT_2785" CDS 12148..12492 /locus_tag="ClothDRAFT_2785" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430924.1" /db_xref="GI:256005983" /translation="MESVNEEFYSIKVEKVLMGNISSDVFKLTNTEYSKVPFLKSGEI RVLSVNEHNGNYGIARGAYQADSTDYKTLNLLQAKEGYIPAKIIQEFINTGAFIEADK KQKRKTFSESMK" gene 12489..12752 /locus_tag="ClothDRAFT_2786" CDS 12489..12752 /locus_tag="ClothDRAFT_2786" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ZP_05430925.1" /db_xref="GI:256005984" /translation="MKQQFKLKPMRHQYRKVIRSIKNVKEASINNEDNENNEKNGALH SVTREADNKYLPIIVIIPLIVVGSFLILVIKQKNVQEFLDKKR" ORIGIN 1 agtttttata taattttatt aagcttttta tttataagtt tatgttatcc aaacacttct 61 atttatgcgc cattaatgat aaaatatata tgtggatagt tttttggcta atcccttata 121 tattcttttt gaattgttta tacagtttag atacaaaaat ggaacctgca gcatgtgaac 181 tatccagaaa aatgtgaagg gtggtatttt aatggatggt tatttaaaac aatatgtggg 241 gaagaaaata gttgtgtatc tttcgctgtg gtctatgctc tttcatgttg gtgtatctgg 301 actggtaagt gattgccggg atggctggat tgagcttaag agaaaaaaca gggttgagca 361 tattaacatt ggtaaaatat attgttttag agttatagag tgaaaatata atgctaatgg 421 gcattcggac gtgaatgccc attagcatta tgttttatga tgtgtatccg tgtttgctgt 481 gtatgattaa aaaccgttgt ttttgattac ttctttgtac cagtagccgc tatcctttat 541 ttttctttcc aacgtatcaa aatttacgtg aacgattccg aatctcttgt tgtacccgta 601 agcccattca aagttgtcca aaagcgacca caagtagtat gcttttaagt ttacaccgtc 661 ctgaattgcc ctgtgagcct gggtcagata atctttaaga tattggattc tctttgtgtc 721 ttctatcttt ccgttgctac ctatttcatc tttgaaggcg gctccgtttt cgctgataac 781 aatgtttggc tttccataat ccctgtcaag gagcataagc agatcataca agccttcagg 841 atatatgatc cagcccatat ctgttttttc gaacttttct aatatggagt ttgcaggtga 901 aaaacctgac tcactggacg gatcatattt tataaattcc gaagaataat tgttgaatgc 961 tatgaagtct attggctgac tgataagttt caggtcatct tcagggaaag aaagctcaat 1021 acccttcttt ttataaagtt tcaatgcgtt ttcaggatac cggcctttta gcacaggatc 1081 cagataccac cttcccgcca gagaaaatga caattccgct gcttcaatat cctcagcttt 1141 ttcggatgcg ggataatggt aagataaatt gagagctatt ccaatttggg cgtcaatatt 1201 catttctcta aacagtttca cggccttgcc gtgggacaaa agaagattgt gcgagacttc 1261 caatgaagtg cggaggtctt ttatcccagg ggcatgaatt cctaaaaagt ggccaagcaa 1321 agaaacaaca ccgggttcat tgtgagtaaa ccatattgga acgatatctc cgagattttt 1381 taaatattac ttcagagtat tctgtaaaat aatcggtggt gtcccggttt ttccatccgc 1441 ctttatcctg aagcttttgg ggaaggtccc agtgataaag agtgattgca ggcataattc 1501 cgttttccag aagcagattt gtgagccttt tgtaaaaatc cagtcccttt tgatttaatt 1561 tacctgttcc ttcaggaaag attctgggcc atgagatgga aaacctgtat gatttaatac 1621 cgatttcttt cattattttg atatcttctt cataacgatg atagtggtcg catgcaacat 1681 cgccggtatg tccgtctgct atatttcctg gcgtgtggga aaaacggtcc catatagatt 1741 cacctttgcc gtcttcgttg tatgcacctt caatctgata tgctgctgtt gcagaacccc 1801 atatgaaatc ttttgggaaa gttatctttg acatgtttac cacacccttt aagatattat 1861 ttgcaaacag tgtaataata taattataac ctagaggaaa cataaatact atttattttt 1921 aatcgaaaaa atgaatagcc tgaaaaagca taaagattat ggtatattat ggtgagttta 1981 tgagtttttt tctttgtatt aaattgaata atgtgataaa atagcaaaat agattgagat 2041 aattaaagaa ttatataaat taaagaatta tatatcaaaa taatatatta aataatatgg 2101 gggtaatgtt tggtggaatt aggcaggtta aagagaaggg cgaggttcca gctaagcaac 2161 aatggagcta atctggcgct tttatatatg atgaccatgg cattttatat tcttttgttt 2221 ttgttgctgg ggcgtttcag ccttcagact gcattaataa ttaacggtcc ggttttgctg 2281 ggatttacaa tttattttct tgctatagca agggtggaaa aagagcccag aacaagaaaa 2341 gaaaaggaag aaaaggataa ggagaaaaag cttaaaggaa aatttactgt cttcgggatg 2401 gaaataaagc ttggaaaaaa gataaaaata aatctcgata cggcggaaca tccttaccgc 2461 gtcagtttaa aatatgtgtt ttacggtttt aaatatttta taaacgctgc tgcgctttat 2521 atctggacta cccttttgat attggtttgg tcgcttttat tgattgttcc tggaataatc 2581 aaggcccttg atttgagtat gagctttttc atattggctg acaatccaac aatggatatc 2641 agaaaagcgg cagatttgag tacgaaaata atgaaaggcc acaggaaaga gttgtttttg 2701 cttaccttga gctttatagg ctggatatta atttcaatat ttactttttc cgttggtttt 2761 gccattacga taccgtatat tatgactacc tatgcacttt tttatgatga ggttaaaaaa 2821 cagagtatag aaaacggaat tattgcagaa gatgaaattg gaaccagaag gttgtgggaa 2881 tattaaaaat cattgaaata tgcgttgaat aatgaaaatg cgcattgaat atatgtaaaa 2941 tttatgctat aatttataaa attaatttat gctgtaattc ataaatttat attcgggcga 3001 tgactgagag agtagatggt catgaaggat ataagagaga agataccata ggctgagagt 3061 atctttatca gttcaggcca ttgaagttcc ctcaggagct gcattctgaa caccggtgga 3121 aaaatggcgt tgcaggcgga gccggtaagt aggaagtgcc gggttgcagc cgttatctgc 3181 aaaagagtgc ttgcatgtct tgaggatatg caagaatttg ggtggtaccg cgaagcaaac 3241 cttcgtccct ttgtattggg atgaaggttt ttttatgaaa caaaagacgg taaaaacact 3301 ttttatgaat ttatataagg actacaatta atgattatat aaggaggaat acaaagcatg 3361 aaggagcagt tgaacagtat cagagttcag gcagagcagg aactttccaa tgttggaacc 3421 attgcggaac tggaaaacat cagagtcaaa tatttgggca aaaaagggga acttaccgcc 3481 gtattaagag gcatgggttc tctgtcgccg gaagaaaggc cggtaatagg ccagctggca 3541 aatgagatta gggcttatat tgagagccgt atagaagagg ccaggaacga gcttataaaa 3601 aaagaaagaa gtcaaaaact cgagagagaa gtcattgacg taaccatgcc gggaaaaaga 3661 aagatgcttg gaaagaaaca tcctttgagc attgtgatag acgagattaa agatgtgttt 3721 ataggaatgg gttacgaaat agccgaaggt cctgaggttg agctggacta ttacaacttt 3781 gaagccctca acattcccag aaaccacccg gcaagagatg tacaggacac tttctacata 3841 aataacaaca tattactcag aacccagaca tctcccgttc agatcagggt tatggagaac 3901 aaaaaaccac cgattaaaat aatctgcccg ggaagggtgt accgctccga tgctgtggat 3961 gccacccatt cacctatatt ccatcaagtg gaaggactgg tggtggacaa gggagttaca 4021 atgggagacc ttgtcggaac tttaagagtg tttgcaaaga gtttgttcgg agaaaagaca 4081 gaaataaggc tgagacctca ccatttccca ttcacagagc ccagtgccga ggttgatgtt 4141 tcatgctggg cttgcggagg aacaggctgc agaatatgta aaaatgaagg ctggatagag 4201 attttgggcg ccggaatggt tcatccgaag gttctggagg tttgcggcat agaccctgaa 4261 gtatacagcg gatttgcttt tggtcttggc gtcgaaagaa cggccatggg aagatttaat 4321 atcgatgaca tgagactttt gtatgaaaac gatatcaggt tcttaaaaca gttttaatct 4381 ctgattttta ccataaaatt tgcgaaagga gtagtagtat gaaagcaccg atagattggc 4441 tgaaagatta tgttgatata aatgtgagca caaaagagct ggcggatgcc atgacaatgt 4501 caggctccaa ggttgaagga atagaagttc agggagaaga aatcaccaaa gttgtggtgg 4561 gaaaaatact ttctcttgaa aagcatcccg atgccgacag gttgcaggtg gcaaaggtgg 4621 atgtgggcag cgaggtatta cagatagtta caggagcaca gaacgtaaat gtcggagatt 4681 acataccggt ggctcttgtg ggttccactc ttcccggagg aaagaaaata tccaaaggaa 4741 aactcagagg tgtggaatcc tacggcatga tgtgctcaat agaagaattg ggccttacca 4801 gggatgattg tcccgatgcg ccggaagacg ggatttatat cctgcccaaa gaagaggaat 4861 tgggtaaaga tattaaagaa gtattgggat tgaacaagga gattattgag tttgaaatta 4921 catccaacag accggactgt cttagtatta ttggtcttgc aagagaagcc gctgtcactt 4981 tgaagacaaa gttcaagagg cctgttataa atgtgaagga agaaggggac gacgcttcgc 5041 agtatatttc cgttgaggtg aaagataccg ggctttgccc gagatttgcg gcaagggttg 5101 taaaggatgt caaaatcgga ccttctccca agtggatgag ggacaggctt aaagctgcag 5161 gagtaagacc gattaacaat attgttgaca taacaaacta tgtaatgctg gaatacggtc 5221 agccgatgca tgcttatgac atcaaggata taaagggaaa caagattatt gtaaggagag 5281 cttttgacgg agagataatc aaaaccttgg atgatcagga cagagaactg gactcctcaa 5341 tgcttgtgat agccgatgag gaaagggctg tcggagtggc cggcgttatg ggcggtgcca 5401 attcggaaat aaaagatgac acgaaaacca tagtatttga ggccgcaaac ttcaacggaa 5461 catccgtgag acttacggca aagaagctgg gaatgaggac cgaagcctcc ggaagatttg 5521 agaaaggcct tgatcctgaa aatgtggagg ctgccatcaa cagggccgcg cagcttgtgg 5581 aagagctggg aataggtacg gtatgcaagg gcatgattga ttgttaccct gaaaaaaaga 5641 gccaagaatt ataaaactca gggttgacaa aataaacagt ctccttggca ccaacatccc 5701 gaaagaagaa atgattgata ttttgagggc cctggagttt gaagtggatg agaataatat 5761 gactgtaaag gttccaactt ttagagatga tgtagaaagg gaagcggaca ttgcggaaga 5821 aattgcaagg ttctacggct acaataacat agaagccacg cttttgtccg gcaagactgc 5881 cacacaggga agaaagacct acaagcagac tattgaagac ctcataaagg aaacaatgat 5941 tgcgtgcgga ctgtgtgaga catatacctt ctcgtttacc agtccgaaag tgtttgacaa 6001 attaaatctt ccggcggaca gcgagcttcg caaggctgtt gttatttcca atccgctggg 6061 agaggattac agcataatga gaaccactac tattccggat atgcttgcgg taataagcac 6121 caactacaac agaagaatag aagaagcaag actgtttgaa atgtccaagg tttatattcc 6181 caagagcctt ccgctggagg agctgcccga ggaaaaaacg gtacttactt tgggaatgta 6241 cggtgagctt gatttctatg atttgaaagg cgttgtggaa gaacttctgg aaaggcttgg 6301 aataaagaat tatgatgttt cgccggagaa gaacaatgtt gtgttccatc cgggaagaac 6361 ggctttaata aacattgacg gtgagtatgc gggtattata ggagaaatac atcctgaagt 6421 tgccgaaaag tttgaatgtc cggagcgcac ttacattggt gtcatcgagg ttgaaacact 6481 ggtcaggaaa gcttcaatgg attgccagta caaagggctt ccgaagtatc cggccgttac 6541 aagggatata gctatgcttg taaaagatga agtaatggta aaagaaatag aagacattat 6601 aaagcagaga gcaggcaaga tacttgagag tgtgaagctt tttgatgttt acaaaggcaa 6661 gcaggtaccc gaaggcatga agagcgtggc ctattccata acgttcaggg catccgacag 6721 gactttgacc gatgaagaag tgggcaaggc tatgacaaag attcttgacg gactgaagag 6781 aaatcttggt gcggagctta gataacaatg aataataaat agccggagaa ggggggagga 6841 gattcttttc tccggctata gtaagctttt gaaatctatt ttattaattc actttttgcc 6901 atttcaatca ttctttttac catttgtccg ccaatgggtc cgccttgcaa tccgtttatt 6961 ctgcatggta cgtcgccttt gtaatggtcg ttgttttctt taacgaactg caaataaccg 7021 atttctttgg cgcattctat tttaaaacgg tttaaagcct ctcttgcttt tgggtccaaa 7081 gaagtatttc ttcttgccat gctattcacc accttgtatg ttttgtatgt atttagtttg 7141 cactttttta aaaaggatta acacagcaat tttaacctcg atttcacatt ggcatgaaga 7201 cggaccatat tttgcgtgag attaaactgt tgaaagattg aattattatg taacaaaatt 7261 gtgtgaacga attgtttaat cttttgaagt tgttattgga gttattgctg ttattattat 7321 tcaagttgga aaaattagaa tactgtattg cgttaagatt aatcatttgt taatataaaa 7381 acaaagttgt tgagaagctt atcagacttg tgtttgattc ataaaaaatt tatgtgctgg 7441 aggtttaacg atggaaagaa taaaatttga ctattcaaaa gcattgcctt ttgtaagtga 7501 acgtgaagtt gcatatttcg agaattttgt aaggtctgcc catgacatgc tccataacaa 7561 aaccggagcg ggaaatgact ttgtaggctg ggttgatctt cctgtaaatt atgacaggga 7621 agaatttgcg agaatcaagg ctgcggcaga aaagataaaa tctgattctg atgctttggt 7681 tgtaattgga atcggaggtt cctatctggg agcaagggcg gcaatagaga tgctttccca 7741 ctcattccac aatctcatgc ccaaatcaaa gaggaatgct cctgagatat attttgtggg 7801 aaacaatatc agctctacat acattgctga tttgctggaa gtaatagaag gcaaagagat 7861 ttcggtaaac gttatatcaa aatccggtac tacaacggag cctgccattg ctttcagaat 7921 ctttaaagag tacatggaaa acaaatacgg aaaagacgga gcaagtaaaa gaatatatgc 7981 cactaccgac aaggagaaag gagcactcag gaagctggca accgaagagg gatatgaaac 8041 atttgtagtt cctgatgaca taggtggaag attctccgtt ctgacggcag ttggcttgct 8101 tcccattgca gtggccggaa ttgacatcga cagcatgatg aagggagctg ctgacgcccg 8161 tgagctttac agcaatccaa acctgatgga aaacgactgc tacaaatatg cggctgtaag 8221 aaacgctctc tacagaaaga acaagacaat tgagataatg gtaaactatg aaccttcact 8281 ccattacttc acagaatggt ggaaacagct ctacggagaa agtgaaggaa aggatcaaaa 8341 aggtatattc ccggccggag ttgacttcac tacggacctt cattccatgg gacagtatat 8401 acaggatgga ctcaggaaca tatttgaaac ggtaatcagg gttgaaaagc ccagaaagaa 8461 tattgttata aaggaagaaa aggacaacct tgacggattg aactttattg ccggaaaaga 8521 cgtggactat gtaaacaaga aagcaatgga aggaacggta cttgcccata ccgacggcgg 8581 tgttccgaat cttgtggtaa ccgtgcctga gcttagtgct tattactttg gaaatatggt 8641 atacttcttt gaaaaagcct gcggtataag cggatacctc cttggtgtaa atccttttga 8701 ccagccggga gttgaggctt acaagaaaaa catgtttgcc cttcttggaa aaccgggata 8761 tgaagaacaa agaaagaaac ttgaagagcg tttgtaagaa atatgtttgt gatttatatg 8821 tgatttataa tttttataat tacggcaaac agataaataa attatggtca atggaataaa 8881 taaaataagg agtcggaaag ctttctgact ccttattttt atagcaaaaa ttaattttat 8941 ggcagatgta gtatggatat ttccggcggt gaaaaaagcc ttaagggaac acacacattt 9001 ccaagtccgc tgttaatatg caagttaatg ttgttaagct tgtgggttcc ttttgtaata 9061 cctattttag taagtttttc atggcgcaaa attctaaatt ccagattaaa aggcatccat 9121 atctgtccgc cgtgaaaatg accgcacaaa agataatcca cacttccccc gggtagttga 9181 agaataatgt cggggttgtg cgatatggct atattggtgc agccttttgt attgcagccg 9241 gcaagagcct tttttacatc ataccgtttt gacctgaggt cctcaattcc aataacattg 9301 tactttcggt tgcccttttc aatacagacg gagctgttga gaaggactga tacacctttt 9361 tcttcaatta gctgtttaaa ctcctgtatt gaatcttcgc tgttttttag tgccttgtaa 9421 tcgtggttgc cgaaacaaag gcaaatttta taatttccct tgatactttc aaggaacttt 9481 ataaatgccg gtatatgttt cggattgtct atgtaatctc ccgtaagtac gacaagatca 9541 ggtttttccg tctctaatac ctttaccacc ttgtttatat ccacgttcag aaactttata 9601 tgcaggtccg acatatgtac aatcttaaaa ggctctttgc tttcggtaaa acagaccctg 9661 ctgattttca gccttgtcgt ttcatagtac atataaacaa gggcaagtat aattaataat 9721 aaaacaaaat acagaatgtt cattgcttta cctttttcgt attttattct tttattattt 9781 tgcttttatt tcacatcaaa taatatatca gtaaatttcg actttatcaa tcgttttttt 9841 taagccggta aaatatgggg atagcttcga accaagtagt tgtgcggctt ttagcaatat 9901 taacctctgg attttatata aatgggagtt gtataattct atttaggggg gtgcttaata 9961 tgttcaattt tcttaacaat gactatgaac tgcagcctag aacgtactcc attaaaagct 10021 atcggaggag ggaatcttcg ataggcaaaa agatggcaag attacttgtt tggcttgtta 10081 tactaataat tttgggtgtg gcggcgtttc ttttctttta ttaatggtct gttaatctga 10141 ttattaattg gcaaaggctt gctttttgta aagagttttg aaggctaaat tctgtattga 10201 caaatagatg gaacaaaagt ataataacct gtgttgcgta atatttttgc ggacgtggcg 10261 gaattggcag acgcgctaga tttaggatct agtgtcaccg acgtgggggt tcaagtccct 10321 tcgtccgcac cataaaggtt tggaaggatt gcaagaattc tttcaaacct tttttcaatt 10381 ggcaggtaag ctgtttagaa attatacatg cgtaatacct gtataattcg gtactacgca 10441 tgtatttcat taaatggggg gaatgttaag ctatgaagaa aatatttact gttgcagtta 10501 tattattaat tattgccaat tgcatacaaa ttatgattat aaaatagaaa aataatcaaa 10561 tttttagatt gaaatataag aaagaagctt tgtatatttt tcaagggtga tatttgccgg 10621 taaattcatg tacgattatt ttgaatacca gcacactgtc tatcatttta tcatcaaatg 10681 aaaagtcttt tccggtttga tgtttcatca atgcagagag ggcagctttc ttttcttcgg 10741 catcatcaat aaagacgact ttaccgtttc cgataatgct tttaaatgaa tacgaatatt 10801 ggcaggctgt gtctcctgta atgagccggt gttcacaatc catttcaaag catacatcgc 10861 tgttttcttt cagtgctcta acttttcttc cgtcttttgc gctatggaaa aataatacca 10921 gctgattgtt ttcataagca taaccaaaat tcattggcac gatataaagg ccggcactgt 10981 cttgaatagc aatcctgcag actttgcact ggtcaataat ctgaattaac tcttttattt 11041 ccgtaacttc tctgtctttt cttcgcaatt tcattgtacc cctttcccta atattgattc 11101 atgttttata gtgaacgtgt ttgaatataa ccagatatat taaatgagtt tgccttttcg 11161 ggttggattt attatatatt ttacatactc aaaccgcaat aatgctcagg aagctgttat 11221 cgcttgctat gtatttgctt gaaagttgtc gtttttcacc attcatttgc tcatattatg 11281 tagttgttga tgtaaaaata atataattat tcaatttaaa tgtaatgatt ttttatataa 11341 aataatttta tggttttaga cctaaacaaa tcttgcttgc catgataaaa atttgataca 11401 ataaagttgt aacaattgac aaatatccgt attttatcaa catgcaaaaa atgtttttat 11461 attgtgtaaa gatgaattgt ttaaaaaaat aaaggtttat gtaagaaggc aactatatag 11521 cttaggattt caaaaagtta ataacttcaa gttttcatct actcacaata cagagcgttc 11581 tattactttg gtattctacc atagaaggag tcaattagtt tgcataaaat attttacagt 11641 ctcaggcatt ataaatatat tttaaggttt aagaatcttg cttgtaaatt tttacatctt 11701 taatattact actgaaaagt gcagtcaaag attataaatt aaggaattta tgaatttcgg 11761 caatgttaat cacttccttg tgctatattt gctcttttta ctaattataa tacaattatt 11821 ttacttaaat tcaacagatt tttattaaat caatttactg gttctagact aaactaaatc 11881 ttgtttatca cggtaagaat ttgatacaat aaatttgtat attttatggt aatttaatta 11941 aattataata aaattaactg tatatatttt atagtaaaag catttgtaga agagagcttt 12001 tctcataaaa ggggaaagtt atatgaaaag agtaataatg atttttgccg tatttatatt 12061 agttgctata tttaatacat ttgtttatgc cggtgaagaa tatcagatga ttatgaacgg 12121 tgcacaatct gctttagcat tggaatagtg gagagcgtaa atgaagaatt ctactcaata 12181 aaagtggaaa aagttttgat ggggaacata tcaagcgatg tatttaaact taccaacacg 12241 gaatactcga aagttccgtt tctaaaatct ggagagattc gggtattatc tgtgaatgaa 12301 cataatggta actatggaat tgcaagggga gcatatcagg ctgacagtac agattataag 12361 acgttgaatt tgttgcaagc taaagaagga tatattccag caaaaataat tcaggagttt 12421 ataaacactg gtgcatttat tgaagctgat aaaaagcaaa agagaaaaac tttcagcgaa 12481 tccatgaagt gaagcaacaa ttcaagttga agcctatgag acaccaatac cgaaaagtaa 12541 tcaggtcgat aaaaaacgtt aaagaagcga gcattaataa tgaggataat gagaataatg 12601 agaaaaacgg agctttgcat tcagtaacta gagaggctga taataaatat ctgcctataa 12661 tcgtaataat tccattgatt gttgttggtt cttttttgat acttgttata aaacaaaaaa 12721 atgtacaaga gtttttagat aagaagcgat aaactttcaa acgaaaggtt tgaaaatata 12781 tagatgatat aacaatatat ataagcatta ttaaaattta tataaatact attaatataa 12841 ttaaataatt aaaataattc aaatggcaac ctttgagaaa tgaaagatag atgagaagaa 12901 cgaaaagtaa ttatatttga agatagcagt gggattattg gctttattgc cggaaagatt 12961 atcaggtgtt atttgcctat ttcaagtatt cgcaaagttg gttgtatatt tggaacttat 13021 attcttctcg aacataggca aaaaggtgtg atgcgaaaaa cgagaaatga aattgtgatg 13081 ttctttaaaa ctaaagatct aaaatatgtt gagttaagtt ttctatagca gaaacgtatt 13141 gcaagaaaaa gtgagaagtt ctggattata aggtgtttag ataacaggca cgccgcctaa 13201 tataaaaatt tccctttgac agggacaaaa gaacgggatt ttatgtttaa gcggcaatga 13261 agtattggat gaaagaagtc agaatcattt ctgcggttaa aagtaggccg caccttgttc 13321 taggggttat ggataatgga gtagttagta caaaagcgtt ataaatgtat atttattgaa 13381 tatgaagggt taagattaac ttacgtttgc aatcgacata ctcagtcatt ataaattaac 13441 acaaaatttt aaagtttaaa gaaaagaagt tctaacttag aaaagctaaa aaaattgtct 13501 tgacaatggg ggattgtatt tttttcttat agtaaagtaa ataagatcaa agtaatggct 13561 tttttgaata ttaaaaaagt tgttgagctt tgaacgatat ggacaagatt atgtttacta 13621 ttgaaactat gataaattgc gttaattgtc tattttaaaa atttataaaa atatataaaa 13681 atagagttcg atttgtaagc tgataaagtc tattttaaaa agttagtatg gaatttagac 13741 aagtcttctg tatattttta taagctatgt aattagtaac tagatataaa tagaaattaa 13801 gcaatttatc gtgattgtaa aaaaataaat agtaataaaa tctagtacgt tcaaagctaa 13861 cttgggatta tctcaaaagc tagttttgga ttaattgaag aatttaaaaa gtgtattgtt 13921 ggggggaaaa gatgttaggt aatattaaac ctgaagggaa acaaaaagat gtattggctc 13981 ttcctaccac aggtca //