/* The MIT License Copyright (c) 2008 Genome Research Ltd (GRL). Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ /* Contact: Heng Li */ #include #include #include #include #include #include #include "bntseq.h" #include "bwa.h" #include "bwt.h" #include "utils.h" #include "rle.h" #include "rope.h" #ifdef _DIVBWT #include "divsufsort.h" #endif #ifdef USE_MALLOC_WRAPPERS # include "malloc_wrap.h" #endif int is_bwt(ubyte_t *T, int n); int64_t bwa_seq_len(const char *fn_pac) { FILE *fp; int64_t pac_len; ubyte_t c; fp = xopen(fn_pac, "rb"); err_fseek(fp, -1, SEEK_END); pac_len = err_ftell(fp); err_fread_noeof(&c, 1, 1, fp); err_fclose(fp); return (pac_len - 1) * 4 + (int)c; } bwt_t *bwt_pac2bwt(const char *fn_pac, int use_is) { bwt_t *bwt; ubyte_t *buf, *buf2; int64_t i, pac_size; FILE *fp; // initialization bwt = (bwt_t*)calloc(1, sizeof(bwt_t)); bwt->seq_len = bwa_seq_len(fn_pac); bwt->bwt_size = (bwt->seq_len + 15) >> 4; fp = xopen(fn_pac, "rb"); // prepare sequence pac_size = (bwt->seq_len>>2) + ((bwt->seq_len&3) == 0? 0 : 1); buf2 = (ubyte_t*)calloc(pac_size, 1); err_fread_noeof(buf2, 1, pac_size, fp); err_fclose(fp); memset(bwt->L2, 0, 5 * 4); buf = (ubyte_t*)calloc(bwt->seq_len + 1, 1); for (i = 0; i < bwt->seq_len; ++i) { buf[i] = buf2[i>>2] >> ((3 - (i&3)) << 1) & 3; ++bwt->L2[1+buf[i]]; } for (i = 2; i <= 4; ++i) bwt->L2[i] += bwt->L2[i-1]; free(buf2); // Burrows-Wheeler Transform if (use_is) { bwt->primary = is_bwt(buf, bwt->seq_len); } else { rope_t *r; int64_t x; rpitr_t itr; const uint8_t *blk; r = rope_init(ROPE_DEF_MAX_NODES, ROPE_DEF_BLOCK_LEN); for (i = bwt->seq_len - 1, x = 0; i >= 0; --i) { int c = buf[i] + 1; x = rope_insert_run(r, x, c, 1, 0) + 1; while (--c >= 0) x += r->c[c]; } bwt->primary = x; rope_itr_first(r, &itr); x = 0; while ((blk = rope_itr_next_block(&itr)) != 0) { const uint8_t *q = blk + 2, *end = blk + 2 + *rle_nptr(blk); while (q < end) { int c = 0; int64_t l; rle_dec1(q, c, l); for (i = 0; i < l; ++i) buf[x++] = c - 1; } } rope_destroy(r); } bwt->bwt = (u_int32_t*)calloc(bwt->bwt_size, 4); for (i = 0; i < bwt->seq_len; ++i) bwt->bwt[i>>4] |= buf[i] << ((15 - (i&15)) << 1); free(buf); return bwt; } int bwa_pac2bwt(int argc, char *argv[]) // the "pac2bwt" command; IMPORTANT: bwt generated at this step CANNOT be used with BWA. bwtupdate is required! { bwt_t *bwt; int c, use_is = 1; while ((c = getopt(argc, argv, "d")) >= 0) { switch (c) { case 'd': use_is = 0; break; default: return 1; } } if (optind + 2 > argc) { fprintf(stderr, "Usage: bwa pac2bwt [-d] \n"); return 1; } bwt = bwt_pac2bwt(argv[optind], use_is); bwt_dump_bwt(argv[optind+1], bwt); bwt_destroy(bwt); return 0; } #define bwt_B00(b, k) ((b)->bwt[(k)>>4]>>((~(k)&0xf)<<1)&3) void bwt_bwtupdate_core(bwt_t *bwt) { bwtint_t i, k, c[4], n_occ; uint32_t *buf; n_occ = (bwt->seq_len + OCC_INTERVAL - 1) / OCC_INTERVAL + 1; bwt->bwt_size += n_occ * sizeof(bwtint_t); // the new size buf = (uint32_t*)calloc(bwt->bwt_size, 4); // will be the new bwt c[0] = c[1] = c[2] = c[3] = 0; for (i = k = 0; i < bwt->seq_len; ++i) { if (i % OCC_INTERVAL == 0) { memcpy(buf + k, c, sizeof(bwtint_t) * 4); k += sizeof(bwtint_t); // in fact: sizeof(bwtint_t)=4*(sizeof(bwtint_t)/4) } if (i % 16 == 0) buf[k++] = bwt->bwt[i/16]; // 16 == sizeof(uint32_t)/2 ++c[bwt_B00(bwt, i)]; } // the last element memcpy(buf + k, c, sizeof(bwtint_t) * 4); xassert(k + sizeof(bwtint_t) == bwt->bwt_size, "inconsistent bwt_size"); // update bwt free(bwt->bwt); bwt->bwt = buf; } int bwa_bwtupdate(int argc, char *argv[]) // the "bwtupdate" command { bwt_t *bwt; if (argc < 2) { fprintf(stderr, "Usage: bwa bwtupdate \n"); return 1; } bwt = bwt_restore_bwt(argv[1]); bwt_bwtupdate_core(bwt); bwt_dump_bwt(argv[1], bwt); bwt_destroy(bwt); return 0; } int bwa_bwt2sa(int argc, char *argv[]) // the "bwt2sa" command { bwt_t *bwt; int c, sa_intv = 32; while ((c = getopt(argc, argv, "i:")) >= 0) { switch (c) { case 'i': sa_intv = atoi(optarg); break; default: return 1; } } if (optind + 2 > argc) { fprintf(stderr, "Usage: bwa bwt2sa [-i %d] \n", sa_intv); return 1; } bwt = bwt_restore_bwt(argv[optind]); bwt_cal_sa(bwt, sa_intv); bwt_dump_sa(argv[optind+1], bwt); bwt_destroy(bwt); return 0; } int bwa_index(int argc, char *argv[]) // the "index" command { int c, algo_type = BWTALGO_AUTO, is_64 = 0, block_size = 10000000; char *prefix = 0, *str; while ((c = getopt(argc, argv, "6a:p:b:")) >= 0) { switch (c) { case 'a': // if -a is not set, algo_type will be determined later if (strcmp(optarg, "rb2") == 0) algo_type = BWTALGO_RB2; else if (strcmp(optarg, "bwtsw") == 0) algo_type = BWTALGO_BWTSW; else if (strcmp(optarg, "is") == 0) algo_type = BWTALGO_IS; else err_fatal(__func__, "unknown algorithm: '%s'.", optarg); break; case 'p': prefix = strdup(optarg); break; case '6': is_64 = 1; break; case 'b': block_size = strtol(optarg, &str, 10); if (*str == 'G' || *str == 'g') block_size *= 1024 * 1024 * 1024; else if (*str == 'M' || *str == 'm') block_size *= 1024 * 1024; else if (*str == 'K' || *str == 'k') block_size *= 1024; break; default: return 1; } } if (optind + 1 > argc) { fprintf(stderr, "\n"); fprintf(stderr, "Usage: bwa index [options] \n\n"); fprintf(stderr, "Options: -a STR BWT construction algorithm: bwtsw, is or rb2 [auto]\n"); fprintf(stderr, " -p STR prefix of the index [same as fasta name]\n"); fprintf(stderr, " -b INT block size for the bwtsw algorithm (effective with -a bwtsw) [%d]\n", block_size); fprintf(stderr, " -6 index files named as .64.* instead of .* \n"); fprintf(stderr, "\n"); fprintf(stderr, "Warning: `-a bwtsw' does not work for short genomes, while `-a is' and\n"); fprintf(stderr, " `-a div' do not work not for long genomes.\n\n"); return 1; } if (prefix == 0) { prefix = malloc(strlen(argv[optind]) + 4); strcpy(prefix, argv[optind]); if (is_64) strcat(prefix, ".64"); } bwa_idx_build(argv[optind], prefix, algo_type, block_size); free(prefix); return 0; } int bwa_idx_build(const char *fa, const char *prefix, int algo_type, int block_size) { extern void bwa_pac_rev_core(const char *fn, const char *fn_rev); char *str, *str2, *str3; clock_t t; int64_t l_pac; str = (char*)calloc(strlen(prefix) + 10, 1); str2 = (char*)calloc(strlen(prefix) + 10, 1); str3 = (char*)calloc(strlen(prefix) + 10, 1); { // nucleotide indexing gzFile fp = xzopen(fa, "r"); t = clock(); if (bwa_verbose >= 3) fprintf(stderr, "[bwa_index] Pack FASTA... "); l_pac = bns_fasta2bntseq(fp, prefix, 0); if (bwa_verbose >= 3) fprintf(stderr, "%.2f sec\n", (float)(clock() - t) / CLOCKS_PER_SEC); err_gzclose(fp); } if (algo_type == 0) algo_type = l_pac > 50000000? 2 : 3; // set the algorithm for generating BWT { strcpy(str, prefix); strcat(str, ".pac"); strcpy(str2, prefix); strcat(str2, ".bwt"); t = clock(); if (bwa_verbose >= 3) fprintf(stderr, "[bwa_index] Construct BWT for the packed sequence...\n"); if (algo_type == 2) bwt_bwtgen2(str, str2, block_size); else if (algo_type == 1 || algo_type == 3) { bwt_t *bwt; bwt = bwt_pac2bwt(str, algo_type == 3); bwt_dump_bwt(str2, bwt); bwt_destroy(bwt); } if (bwa_verbose >= 3) fprintf(stderr, "[bwa_index] %.2f seconds elapse.\n", (float)(clock() - t) / CLOCKS_PER_SEC); } { bwt_t *bwt; strcpy(str, prefix); strcat(str, ".bwt"); t = clock(); if (bwa_verbose >= 3) fprintf(stderr, "[bwa_index] Update BWT... "); bwt = bwt_restore_bwt(str); bwt_bwtupdate_core(bwt); bwt_dump_bwt(str, bwt); bwt_destroy(bwt); if (bwa_verbose >= 3) fprintf(stderr, "%.2f sec\n", (float)(clock() - t) / CLOCKS_PER_SEC); } { gzFile fp = xzopen(fa, "r"); t = clock(); if (bwa_verbose >= 3) fprintf(stderr, "[bwa_index] Pack forward-only FASTA... "); l_pac = bns_fasta2bntseq(fp, prefix, 1); if (bwa_verbose >= 3) fprintf(stderr, "%.2f sec\n", (float)(clock() - t) / CLOCKS_PER_SEC); err_gzclose(fp); } { bwt_t *bwt; strcpy(str, prefix); strcat(str, ".bwt"); strcpy(str3, prefix); strcat(str3, ".sa"); t = clock(); if (bwa_verbose >= 3) fprintf(stderr, "[bwa_index] Construct SA from BWT and Occ... "); bwt = bwt_restore_bwt(str); bwt_cal_sa(bwt, 32); bwt_dump_sa(str3, bwt); bwt_destroy(bwt); if (bwa_verbose >= 3) fprintf(stderr, "%.2f sec\n", (float)(clock() - t) / CLOCKS_PER_SEC); } free(str3); free(str2); free(str); return 0; }