#include #include #include #include #define __STDC_LIMIT_MACROS #include "bseq.h" #include "kvec.h" #include "kseq.h" KSEQ_INIT2(, gzFile, gzread) unsigned char seq_comp_table[256] = { 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 'T', 'V', 'G', 'H', 'E', 'F', 'C', 'D', 'I', 'J', 'M', 'L', 'K', 'N', 'O', 'P', 'Q', 'Y', 'S', 'A', 'A', 'B', 'W', 'X', 'R', 'Z', 91, 92, 93, 94, 95, 96, 't', 'v', 'g', 'h', 'e', 'f', 'c', 'd', 'i', 'j', 'm', 'l', 'k', 'n', 'o', 'p', 'q', 'y', 's', 'a', 'a', 'b', 'w', 'x', 'r', 'z', 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255 }; #define CHECK_PAIR_THRES 1000000 struct mm_bseq_file_s { gzFile fp; kseq_t *ks; mm_bseq1_t s; }; mm_bseq_file_t *mm_bseq_open(const char *fn) { mm_bseq_file_t *fp; gzFile f; f = fn && strcmp(fn, "-")? gzopen(fn, "r") : gzdopen(0, "r"); if (f == 0) return 0; fp = (mm_bseq_file_t*)calloc(1, sizeof(mm_bseq_file_t)); fp->fp = f; fp->ks = kseq_init(fp->fp); return fp; } void mm_bseq_close(mm_bseq_file_t *fp) { kseq_destroy(fp->ks); gzclose(fp->fp); free(fp); } static inline char *kstrdup(const kstring_t *s) { char *t; t = (char*)malloc(s->l + 1); memcpy(t, s->s, s->l + 1); return t; } static inline void kseq2bseq(kseq_t *ks, mm_bseq1_t *s, int with_qual, int with_comment) { int i; if (ks->name.l == 0) fprintf(stderr, "[WARNING]\033[1;31m empty sequence name in the input.\033[0m\n"); s->name = kstrdup(&ks->name); s->seq = kstrdup(&ks->seq); for (i = 0; i < (int)ks->seq.l; ++i) // convert U to T if (s->seq[i] == 'u' || s->seq[i] == 'U') --s->seq[i]; s->qual = with_qual && ks->qual.l? kstrdup(&ks->qual) : 0; s->comment = with_comment && ks->comment.l? kstrdup(&ks->comment) : 0; s->l_seq = ks->seq.l; } mm_bseq1_t *mm_bseq_read3(mm_bseq_file_t *fp, int64_t chunk_size, int with_qual, int with_comment, int frag_mode, int *n_) { int64_t size = 0; int ret; kvec_t(mm_bseq1_t) a = {0,0,0}; kseq_t *ks = fp->ks; *n_ = 0; if (fp->s.seq) { kv_resize(mm_bseq1_t, 0, a, 256); kv_push(mm_bseq1_t, 0, a, fp->s); size = fp->s.l_seq; memset(&fp->s, 0, sizeof(mm_bseq1_t)); } while ((ret = kseq_read(ks)) >= 0) { mm_bseq1_t *s; assert(ks->seq.l <= INT32_MAX); if (a.m == 0) kv_resize(mm_bseq1_t, 0, a, 256); kv_pushp(mm_bseq1_t, 0, a, &s); kseq2bseq(ks, s, with_qual, with_comment); size += s->l_seq; if (size >= chunk_size) { if (frag_mode && a.a[a.n-1].l_seq < CHECK_PAIR_THRES) { while ((ret = kseq_read(ks)) >= 0) { kseq2bseq(ks, &fp->s, with_qual, with_comment); if (mm_qname_same(fp->s.name, a.a[a.n-1].name)) { kv_push(mm_bseq1_t, 0, a, fp->s); memset(&fp->s, 0, sizeof(mm_bseq1_t)); } else break; } } break; } } if (ret < -1) { if (a.n) fprintf(stderr, "[WARNING]\033[1;31m failed to parse the FASTA/FASTQ record next to '%s'. Continue anyway.\033[0m\n", a.a[a.n-1].name); else fprintf(stderr, "[WARNING]\033[1;31m failed to parse the first FASTA/FASTQ record. Continue anyway.\033[0m\n"); } *n_ = a.n; return a.a; } mm_bseq1_t *mm_bseq_read2(mm_bseq_file_t *fp, int64_t chunk_size, int with_qual, int frag_mode, int *n_) { return mm_bseq_read3(fp, chunk_size, with_qual, 0, frag_mode, n_); } mm_bseq1_t *mm_bseq_read(mm_bseq_file_t *fp, int64_t chunk_size, int with_qual, int *n_) { return mm_bseq_read2(fp, chunk_size, with_qual, 0, n_); } mm_bseq1_t *mm_bseq_read_frag2(int n_fp, mm_bseq_file_t **fp, int64_t chunk_size, int with_qual, int with_comment, int *n_) { int i; int64_t size = 0; kvec_t(mm_bseq1_t) a = {0,0,0}; *n_ = 0; if (n_fp < 1) return 0; while (1) { int n_read = 0; for (i = 0; i < n_fp; ++i) if (kseq_read(fp[i]->ks) >= 0) ++n_read; if (n_read < n_fp) { if (n_read > 0) fprintf(stderr, "[W::%s]\033[1;31m query files have different number of records; extra records skipped.\033[0m\n", __func__); break; // some file reaches the end } if (a.m == 0) kv_resize(mm_bseq1_t, 0, a, 256); for (i = 0; i < n_fp; ++i) { mm_bseq1_t *s; kv_pushp(mm_bseq1_t, 0, a, &s); kseq2bseq(fp[i]->ks, s, with_qual, with_comment); size += s->l_seq; } if (size >= chunk_size) break; } *n_ = a.n; return a.a; } mm_bseq1_t *mm_bseq_read_frag(int n_fp, mm_bseq_file_t **fp, int64_t chunk_size, int with_qual, int *n_) { return mm_bseq_read_frag2(n_fp, fp, chunk_size, with_qual, 0, n_); } int mm_bseq_eof(mm_bseq_file_t *fp) { return (ks_eof(fp->ks->f) && fp->s.seq == 0); }