#include #include #include #include "minimap.h" #include "mmpriv.h" #include "kalloc.h" static const char LogTable256[256] = { #define LT(n) n, n, n, n, n, n, n, n, n, n, n, n, n, n, n, n -1, 0, 1, 1, 2, 2, 2, 2, 3, 3, 3, 3, 3, 3, 3, 3, LT(4), LT(5), LT(5), LT(6), LT(6), LT(6), LT(6), LT(7), LT(7), LT(7), LT(7), LT(7), LT(7), LT(7), LT(7) }; static inline int ilog2_32(uint32_t v) { register uint32_t t, tt; if ((tt = v>>16)) return (t = tt>>8) ? 24 + LogTable256[t] : 16 + LogTable256[tt]; return (t = v>>8) ? 8 + LogTable256[t] : LogTable256[v]; } mm128_t *mm_chain_dp(int max_dist_x, int max_dist_y, int bw, int max_skip, int min_cnt, int min_sc, int is_cdna, int n_segs, int64_t n, mm128_t *a, int *n_u_, uint64_t **_u, void *km) { // TODO: make sure this works when n has more than 32 bits int32_t k, *f, *p, *t, *v, n_u, n_v; int64_t i, j, st = 0; uint64_t *u, *u2, sum_qspan = 0; float avg_qspan; mm128_t *b, *w; if (_u) *_u = 0, *n_u_ = 0; f = (int32_t*)kmalloc(km, n * 4); p = (int32_t*)kmalloc(km, n * 4); t = (int32_t*)kmalloc(km, n * 4); v = (int32_t*)kmalloc(km, n * 4); memset(t, 0, n * 4); for (i = 0; i < n; ++i) sum_qspan += a[i].y>>32&0xff; avg_qspan = (float)sum_qspan / n; // fill the score and backtrack arrays for (i = 0; i < n; ++i) { uint64_t ri = a[i].x; int64_t max_j = -1; int32_t qi = (int32_t)a[i].y, q_span = a[i].y>>32&0xff; // NB: only 8 bits of span is used!!! int32_t max_f = q_span, n_skip = 0, min_d; int32_t sidi = (a[i].y & MM_SEED_SEG_MASK) >> MM_SEED_SEG_SHIFT; while (st < i && ri > a[st].x + max_dist_x) ++st; for (j = i - 1; j >= st; --j) { int64_t dr = ri - a[j].x; int32_t dq = qi - (int32_t)a[j].y, dd, sc, log_dd; int32_t sidj = (a[j].y & MM_SEED_SEG_MASK) >> MM_SEED_SEG_SHIFT; if ((sidi == sidj && dr == 0) || dq <= 0) continue; // don't skip if an anchor is used by multiple segments; see below if ((sidi == sidj && dq > max_dist_y) || dq > max_dist_x) continue; dd = dr > dq? dr - dq : dq - dr; if (sidi == sidj && dd > bw) continue; if (n_segs > 1 && !is_cdna && sidi == sidj && dr > max_dist_y) continue; min_d = dq < dr? dq : dr; sc = min_d > q_span? q_span : dq < dr? dq : dr; log_dd = dd? ilog2_32(dd) : 0; if (is_cdna || sidi != sidj) { int c_log, c_lin; c_lin = (int)(dd * .01 * avg_qspan); c_log = log_dd; if (sidi != sidj && dr == 0) ++sc; // possibly due to overlapping paired ends; give a minor bonus else if (dr > dq || sidi != sidj) sc -= c_lin < c_log? c_lin : c_log; else sc -= c_lin + (c_log>>1); } else sc -= (int)(dd * .01 * avg_qspan) + (log_dd>>1); sc += f[j]; if (sc > max_f) { max_f = sc, max_j = j; if (n_skip > 0) --n_skip; } else if (t[j] == i) { if (++n_skip > max_skip) break; } if (p[j] >= 0) t[p[j]] = i; } f[i] = max_f, p[i] = max_j; v[i] = max_j >= 0 && v[max_j] > max_f? v[max_j] : max_f; // v[] keeps the peak score up to i; f[] is the score ending at i, not always the peak } // find the ending positions of chains memset(t, 0, n * 4); for (i = 0; i < n; ++i) if (p[i] >= 0) t[p[i]] = 1; for (i = n_u = 0; i < n; ++i) if (t[i] == 0 && v[i] >= min_sc) ++n_u; if (n_u == 0) { kfree(km, a); kfree(km, f); kfree(km, p); kfree(km, t); kfree(km, v); return 0; } u = (uint64_t*)kmalloc(km, n_u * 8); for (i = n_u = 0; i < n; ++i) { if (t[i] == 0 && v[i] >= min_sc) { j = i; while (j >= 0 && f[j] < v[j]) j = p[j]; // find the peak that maximizes f[] if (j < 0) j = i; // TODO: this should really be assert(j>=0) u[n_u++] = (uint64_t)f[j] << 32 | j; } } radix_sort_64(u, u + n_u); for (i = 0; i < n_u>>1; ++i) { // reverse, s.t. the highest scoring chain is the first uint64_t t = u[i]; u[i] = u[n_u - i - 1], u[n_u - i - 1] = t; } // backtrack memset(t, 0, n * 4); for (i = n_v = k = 0; i < n_u; ++i) { // starting from the highest score int32_t n_v0 = n_v, k0 = k; j = (int32_t)u[i]; do { v[n_v++] = j; t[j] = 1; j = p[j]; } while (j >= 0 && t[j] == 0); if (j < 0) { if (n_v - n_v0 >= min_cnt) u[k++] = u[i]>>32<<32 | (n_v - n_v0); } else if ((int32_t)(u[i]>>32) - f[j] >= min_sc) { if (n_v - n_v0 >= min_cnt) u[k++] = ((u[i]>>32) - f[j]) << 32 | (n_v - n_v0); } if (k0 == k) n_v = n_v0; // no new chain added, reset } *n_u_ = n_u = k, *_u = u; // NB: note that u[] may not be sorted by score here // free temporary arrays kfree(km, f); kfree(km, p); kfree(km, t); // write the result to b[] b = (mm128_t*)kmalloc(km, n_v * sizeof(mm128_t)); for (i = 0, k = 0; i < n_u; ++i) { int32_t k0 = k, ni = (int32_t)u[i]; for (j = 0; j < ni; ++j) b[k] = a[v[k0 + (ni - j - 1)]], ++k; } kfree(km, v); // sort u[] and a[] by a[].x, such that adjacent chains may be joined (required by mm_join_long) w = (mm128_t*)kmalloc(km, n_u * sizeof(mm128_t)); for (i = k = 0; i < n_u; ++i) { w[i].x = b[k].x, w[i].y = (uint64_t)k<<32|i; k += (int32_t)u[i]; } radix_sort_128x(w, w + n_u); u2 = (uint64_t*)kmalloc(km, n_u * 8); for (i = k = 0; i < n_u; ++i) { int32_t j = (int32_t)w[i].y, n = (int32_t)u[j]; u2[i] = u[j]; memcpy(&a[k], &b[w[i].y>>32], n * sizeof(mm128_t)); k += n; } memcpy(u, u2, n_u * 8); memcpy(b, a, k * sizeof(mm128_t)); // write _a_ to _b_ and deallocate _a_ because _a_ is oversized, sometimes a lot kfree(km, a); kfree(km, w); kfree(km, u2); return b; }