#include #include #include #include #define __STDC_LIMIT_MACROS #include "kvec.h" #include "mmpriv.h" unsigned char seq_nt4_table[256] = { 0, 1, 2, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 4, 1, 4, 4, 4, 2, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 3, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 4, 1, 4, 4, 4, 2, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 3, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4 }; static inline uint64_t hash64(uint64_t key, uint64_t mask) { key = (~key + (key << 21)) & mask; // key = (key << 21) - key - 1; key = key ^ key >> 24; key = ((key + (key << 3)) + (key << 8)) & mask; // key * 265 key = key ^ key >> 14; key = ((key + (key << 2)) + (key << 4)) & mask; // key * 21 key = key ^ key >> 28; key = (key + (key << 31)) & mask; return key; } typedef struct { // a simplified version of kdq int front, count; int a[32]; } tiny_queue_t; static inline void tq_push(tiny_queue_t *q, int x) { q->a[((q->count++) + q->front) & 0x1f] = x; } static inline int tq_shift(tiny_queue_t *q) { int x; if (q->count == 0) return -1; x = q->a[q->front++]; q->front &= 0x1f; --q->count; return x; } /** * Find symmetric (w,k)-minimizers on a DNA sequence * * @param km thread-local memory pool; using NULL falls back to malloc() * @param str DNA sequence * @param len length of $str * @param w find a minimizer for every $w consecutive k-mers * @param k k-mer size * @param rid reference ID; will be copied to the output $p array * @param is_hpc homopolymer-compressed or not * @param p minimizers * p->a[i].x = kMer<<8 | kmerSpan * p->a[i].y = rid<<32 | lastPos<<1 | strand * where lastPos is the position of the last base of the i-th minimizer, * and strand indicates whether the minimizer comes from the top or the bottom strand. * Callers may want to set "p->n = 0"; otherwise results are appended to p */ void mm_sketch(void *km, const char *str, int len, int w, int k, uint32_t rid, int is_hpc, mm128_v *p) { uint64_t shift1 = 2 * (k - 1), mask = (1ULL<<2*k) - 1, kmer[2] = {0,0}; int i, j, l, buf_pos, min_pos, kmer_span = 0; mm128_t buf[256], min = { UINT64_MAX, UINT64_MAX }; tiny_queue_t tq; assert(len > 0 && (w > 0 && w < 256) && (k > 0 && k <= 28)); // 56 bits for k-mer; could use long k-mers, but 28 enough in practice memset(buf, 0xff, w * 16); memset(&tq, 0, sizeof(tiny_queue_t)); kv_resize(mm128_t, km, *p, p->n + len/w); for (i = l = buf_pos = min_pos = 0; i < len; ++i) { int c = seq_nt4_table[(uint8_t)str[i]]; mm128_t info = { UINT64_MAX, UINT64_MAX }; if (c < 4) { // not an ambiguous base int z; if (is_hpc) { int skip_len = 1; if (i + 1 < len && seq_nt4_table[(uint8_t)str[i + 1]] == c) { for (skip_len = 2; i + skip_len < len; ++skip_len) if (seq_nt4_table[(uint8_t)str[i + skip_len]] != c) break; i += skip_len - 1; // put $i at the end of the current homopolymer run } tq_push(&tq, skip_len); kmer_span += skip_len; if (tq.count > k) kmer_span -= tq_shift(&tq); } else kmer_span = l + 1 < k? l + 1 : k; kmer[0] = (kmer[0] << 2 | c) & mask; // forward k-mer kmer[1] = (kmer[1] >> 2) | (3ULL^c) << shift1; // reverse k-mer if (kmer[0] == kmer[1]) continue; // skip "symmetric k-mers" as we don't know it strand z = kmer[0] < kmer[1]? 0 : 1; // strand ++l; if (l >= k && kmer_span < 256) { info.x = hash64(kmer[z], mask) << 8 | kmer_span; info.y = (uint64_t)rid<<32 | (uint32_t)i<<1 | z; } } else l = 0, tq.count = tq.front = 0, kmer_span = 0; buf[buf_pos] = info; // need to do this here as appropriate buf_pos and buf[buf_pos] are needed below if (l == w + k - 1 && min.x != UINT64_MAX) { // special case for the first window - because identical k-mers are not stored yet for (j = buf_pos + 1; j < w; ++j) if (min.x == buf[j].x && buf[j].y != min.y) kv_push(mm128_t, km, *p, buf[j]); for (j = 0; j < buf_pos; ++j) if (min.x == buf[j].x && buf[j].y != min.y) kv_push(mm128_t, km, *p, buf[j]); } if (info.x <= min.x) { // a new minimum; then write the old min if (l >= w + k && min.x != UINT64_MAX) kv_push(mm128_t, km, *p, min); min = info, min_pos = buf_pos; } else if (buf_pos == min_pos) { // old min has moved outside the window if (l >= w + k - 1 && min.x != UINT64_MAX) kv_push(mm128_t, km, *p, min); for (j = buf_pos + 1, min.x = UINT64_MAX; j < w; ++j) // the two loops are necessary when there are identical k-mers if (min.x >= buf[j].x) min = buf[j], min_pos = j; // >= is important s.t. min is always the closest k-mer for (j = 0; j <= buf_pos; ++j) if (min.x >= buf[j].x) min = buf[j], min_pos = j; if (l >= w + k - 1 && min.x != UINT64_MAX) { // write identical k-mers for (j = buf_pos + 1; j < w; ++j) // these two loops make sure the output is sorted if (min.x == buf[j].x && min.y != buf[j].y) kv_push(mm128_t, km, *p, buf[j]); for (j = 0; j <= buf_pos; ++j) if (min.x == buf[j].x && min.y != buf[j].y) kv_push(mm128_t, km, *p, buf[j]); } } if (++buf_pos == w) buf_pos = 0; } if (min.x != UINT64_MAX) kv_push(mm128_t, km, *p, min); }