Aligning contigs to reference... Aligning contigs to the reference Filtering alignments... Parsing coords... Loading reference... Loaded [gi_49175990_ref_NC_000913.2_] Total Regions: 1 Total Region Length: 10000 Analyzing contigs... CONTIG: contig1 (1120bp) Best alignment score: 1120.0 (LEN: 1120, IDY: 100.00), Total number of alignments: 1 One align captures most of this contig: 1 1120 | 1 1120 | 1120 1120 | 100.0 | gi_49175990_ref_NC_000913.2_ contig1 CONTIG: contig2 (1610bp) Best alignment score: 1610.0 (LEN: 1610, IDY: 100.00), Total number of alignments: 1 One align captures most of this contig: 1681 3290 | 1 1610 | 1610 1610 | 100.0 | gi_49175990_ref_NC_000913.2_ contig2 CONTIG: contig3 (3980bp) Best alignment score: 2030.0 (LEN: 2030, IDY: 100.00), Total number of alignments: 2 Looking for the best set of alignments (out of 2 total alignments) The best set is below. Score: 3779.9, number of alignments: 2, unaligned bases: 0 This contig is misassembled. Excluding internal overlap of size 2 between Alignment 1 and 2: 8049 10000 | 2029 3980 | 1952 1952 --> 8051 10000 | 2031 3980 | 1950 1950 Real Alignment 1: 4621 6650 | 1 2030 | 2030 2030 | 100.0 | gi_49175990_ref_NC_000913.2_ contig3 Extensive misassembly (relocation, inconsistency = 1400) between these two alignments Real Alignment 2: 8051 10000 | 2031 3980 | 1950 1950 | 100.0 | gi_49175990_ref_NC_000913.2_ contig3 Analyzing coverage... Writing SNPs into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/contigs_reports/minimap_output/contigs_1.used_snps Analysis is finished! Founded SNPs were written into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/contigs_reports/minimap_output/contigs_1.used_snps Results: Local Misassemblies: 0 Misassemblies: 1 Relocations: 1 Translocations: 0 Inversions: 0 Scaffold gap extensive misassemblies: 0 Scaffold gap local misassemblies: 0 Misassembled Contigs: 1 Misassembled Contig Bases: 3980 Misassemblies Inter-Contig Overlap ("Extra" Aligned Bases): 0 Unaligned Contigs: 0 + 0 part Half Unaligned Contigs with Misassemblies: 0 Unaligned Bases in Fully and Partially Unaligned Contigs: 0 Ambiguously Mapped Contigs: 0 Total Bases in Ambiguously Mapped Contigs: 0 "Extra" Aligned Bases in Ambiguously Mapped Contigs: 0 Note that --allow-ambiguity option was set to "one" and only first alignment per each of these contigs was used (there is no "extra" aligned bases). Covered Bases in Reference: 6710 Raw Aligned Bases in Assembly: 6710 Total Aligned Bases in Assembly (with "Extras"): 6710 SNPs: 0 Insertions: 0 Deletions: 0