Aligning contigs to reference... Aligning contigs to the reference Filtering alignments... Parsing coords... Loading reference... Loaded [gi_49175990_ref_NC_000913.2_] Total Regions: 1 Total Region Length: 10000 Analyzing contigs... CONTIG: contig1 (2100bp) Best alignment score: 2100.0 (LEN: 2100, IDY: 100.00), Total number of alignments: 1 One align captures most of this contig: 1121 3220 | 1 2100 | 2100 2100 | 100.0 | gi_49175990_ref_NC_000913.2_ contig1 CONTIG: contig2 (3360bp) Best alignment score: 1471.0 (LEN: 1471, IDY: 100.00), Total number of alignments: 3 Looking for the best set of alignments (out of 3 total alignments) The best set is below. Score: 3023.2, number of alignments: 3, unaligned bases: 0 This contig is misassembled. Real Alignment 1: 3431 4130 | 1 700 | 700 700 | 100.0 | gi_49175990_ref_NC_000913.2_ contig2 Extensive misassembly (relocation, inconsistency = 1750) between these two alignments Excluding internal overlap of size 1 between Alignment 2 and 3: 5881 7070 | 701 1890 | 1190 1190 --> 5881 7069 | 701 1889 | 1189 1189 Real Alignment 2: 5881 7069 | 701 1889 | 1189 1189 | 100.0 | gi_49175990_ref_NC_000913.2_ contig2 Extensive misassembly (relocation, inconsistency = 1050) between these two alignments Real Alignment 3: 8120 9590 | 1890 3360 | 1471 1471 | 100.0 | gi_49175990_ref_NC_000913.2_ contig2 Analyzing coverage... Writing SNPs into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/contigs_reports/minimap_output/contigs_2.used_snps Analysis is finished! Founded SNPs were written into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/contigs_reports/minimap_output/contigs_2.used_snps Results: Local Misassemblies: 0 Misassemblies: 2 Relocations: 2 Translocations: 0 Inversions: 0 Scaffold gap extensive misassemblies: 0 Scaffold gap local misassemblies: 0 Misassembled Contigs: 1 Misassembled Contig Bases: 3360 Misassemblies Inter-Contig Overlap ("Extra" Aligned Bases): 0 Unaligned Contigs: 0 + 0 part Half Unaligned Contigs with Misassemblies: 0 Unaligned Bases in Fully and Partially Unaligned Contigs: 0 Ambiguously Mapped Contigs: 0 Total Bases in Ambiguously Mapped Contigs: 0 "Extra" Aligned Bases in Ambiguously Mapped Contigs: 0 Note that --allow-ambiguity option was set to "one" and only first alignment per each of these contigs was used (there is no "extra" aligned bases). Covered Bases in Reference: 5460 Raw Aligned Bases in Assembly: 5459 Total Aligned Bases in Assembly (with "Extras"): 5459 SNPs: 0 Insertions: 0 Deletions: 0