Aligning contigs to reference... Aligning contigs to the reference Filtering alignments... Parsing coords... Loading reference... Loaded [meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa] Total Regions: 1 Total Region Length: 59999 Analyzing contigs... CONTIG: NODE_8_length_182_cov_2.81102 (1152bp) Best alignment score: 638.0 (LEN: 638, IDY: 100.00), Total number of alignments: 2 Looking for the best set of alignments (out of 2 total alignments) The best set is below. Score: 796.1, number of alignments: 2, unaligned bases: 336 This contig is misassembled. Real Alignment 1: 3395 3573 | 182 5 | 179 178 | 91.62 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_8_length_182_cov_2.81102 Scaffold gap between these two alignments, gap lengths difference (reference vs assembly) = 1020 (extensive) Real Alignment 2: 1717 2354 | 840 203 | 638 638 | 100.0 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_8_length_182_cov_2.81102 CONTIG: NODE_15_length_55106_cov_26.0999 (55106bp) Best alignment score: 55106.0 (LEN: 55106, IDY: 100.00), Total number of alignments: 1 One align captures most of this contig: 4893 59998 | 1 55106 | 55106 55106 | 100.0 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_15_length_55106_cov_26.0999 CONTIG: NODE_33_length_929_cov_25.7529 (929bp) Best alignment score: 929.0 (LEN: 929, IDY: 100.00), Total number of alignments: 1 One align captures most of this contig: 844 1772 | 1 929 | 929 929 | 100.0 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_33_length_929_cov_25.7529 CONTIG: NODE_35_length_850_cov_25.8629 (850bp) Best alignment score: 836.0 (LEN: 850, IDY: 98.35), Total number of alignments: 1 One align captures most of this contig: 3 852 | 1 850 | 850 850 | 98.35 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_35_length_850_cov_25.8629 CONTIG: NODE_39_length_1141_cov_25.1602 (1141bp) Best alignment score: 1141.0 (LEN: 1141, IDY: 100.00), Total number of alignments: 1 One align captures most of this contig: 2300 3440 | 1 1141 | 1141 1141 | 100.0 | meta_ref_2_Lactobacillus_delbrueckii_bulgaricus_ATCC_BAA-365_32_60k.fa NODE_39_length_1141_cov_25.1602 Analyzing coverage... Writing SNPs into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/runs_per_reference/meta_ref_2/contigs_reports/minimap_output/meta_contigs_1.used_snps Analysis is finished! Founded SNPs were written into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/runs_per_reference/meta_ref_2/contigs_reports/minimap_output/meta_contigs_1.used_snps Results: Local Misassemblies: 0 Misassemblies: 0 Relocations: 0 Translocations: 0 Inversions: 0 Scaffold gap extensive misassemblies: 1 Scaffold gap local misassemblies: 0 Misassembled Contigs: 0 Misassembled Contig Bases: 0 Misassemblies Inter-Contig Overlap ("Extra" Aligned Bases): 0 Unaligned Contigs: 0 + 0 part Half Unaligned Contigs with Misassemblies: 0 Unaligned Bases in Fully and Partially Unaligned Contigs: 0 Ambiguously Mapped Contigs: 0 Total Bases in Ambiguously Mapped Contigs: 0 "Extra" Aligned Bases in Ambiguously Mapped Contigs: 0 Note that --allow-ambiguity option was set to "one" and only first alignment per each of these contigs was used (there is no "extra" aligned bases). Covered Bases in Reference: 58677 Raw Aligned Bases in Assembly: 58842 Total Aligned Bases in Assembly (with "Extras"): 58842 SNPs: 28 Insertions: 0 Deletions: 1