/* dict.c -- create a sequence dictionary file. Copyright (C) 2015 Genome Research Ltd. Author: Shane McCarthy Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include "htslib/kseq.h" #include "htslib/hts.h" KSEQ_INIT(gzFile, gzread) typedef struct _args_t { char *output_fname, *fname; char *assembly, *species, *uri; int header; } args_t; static void write_dict(const char *fn, args_t *args) { hts_md5_context *md5; int l, i, k; gzFile fp; kseq_t *seq; unsigned char digest[16]; char hex[33]; fp = strcmp(fn, "-") ? gzopen(fn, "r") : gzdopen(fileno(stdin), "r"); if (fp == 0) { fprintf(stderr, "dict: %s: No such file or directory\n", fn); exit(1); } FILE *out = stdout; if (args->output_fname) { out = fopen(args->output_fname, "w"); if (out == NULL) { fprintf(stderr, "dict: %s: Cannot open file for writing\n", args->output_fname); exit(1); } } if (!(md5 = hts_md5_init())) exit(1); seq = kseq_init(fp); if (args->header) fprintf(out, "@HD\tVN:1.0\tSO:unsorted\n"); while ((l = kseq_read(seq)) >= 0) { for (i = k = 0; i < seq->seq.l; ++i) { if (seq->seq.s[i] >= '!' && seq->seq.s[i] <= '~') seq->seq.s[k++] = toupper(seq->seq.s[i]); } hts_md5_reset(md5); hts_md5_update(md5, (unsigned char*)seq->seq.s, k); hts_md5_final(digest, md5); hts_md5_hex(hex, digest); fprintf(out, "@SQ\tSN:%s\tLN:%d\tM5:%s", seq->name.s, k, hex); if (args->uri) fprintf(out, "\tUR:%s", args->uri); else if (strcmp(fn, "-") != 0) { #ifdef _WIN32 char *real_path = _fullpath(NULL, fn, PATH_MAX); #else char *real_path = realpath(fn, NULL); #endif fprintf(out, "\tUR:file://%s", real_path); free(real_path); } if (args->assembly) fprintf(out, "\tAS:%s", args->assembly); if (args->species) fprintf(out, "\tSP:%s", args->species); fprintf(out, "\n"); } kseq_destroy(seq); hts_md5_destroy(md5); if (args->output_fname) fclose(out); } static int dict_usage(void) { fprintf(stderr, "\n"); fprintf(stderr, "About: Create a sequence dictionary file from a fasta file\n"); fprintf(stderr, "Usage: samtools dict [options] \n\n"); fprintf(stderr, "Options: -a, --assembly STR assembly\n"); fprintf(stderr, " -H, --no-header do not print @HD line\n"); fprintf(stderr, " -o, --output STR file to write out dict file [stdout]\n"); fprintf(stderr, " -s, --species STR species\n"); fprintf(stderr, " -u, --uri STR URI [file:///abs/path/to/file.fa]\n"); fprintf(stderr, "\n"); return 1; } int dict_main(int argc, char *argv[]) { args_t *args = (args_t*) calloc(1,sizeof(args_t)); args->header = 1; static const struct option loptions[] = { {"help", no_argument, NULL, 'h'}, {"no-header", no_argument, NULL, 'H'}, {"assembly", required_argument, NULL, 'a'}, {"species", required_argument, NULL, 's'}, {"uri", required_argument, NULL, 'u'}, {"output", required_argument, NULL, 'o'}, {NULL, 0, NULL, 0} }; int c; while ( (c=getopt_long(argc,argv,"?hHa:s:u:o:",loptions,NULL))>0 ) { switch (c) { case 'a': args->assembly = optarg; break; case 's': args->species = optarg; break; case 'u': args->uri = optarg; break; case 'o': args->output_fname = optarg; break; case 'H': args->header = 0; break; case 'h': return dict_usage(); default: return dict_usage(); } } char *fname = NULL; if ( optind>=argc ) { if ( !isatty(STDIN_FILENO) ) fname = "-"; // reading from stdin else return dict_usage(); } else fname = argv[optind]; write_dict(fname, args); free(args); return 0; }