/* maq2sam.c -- convert MAQ output to SAM. Copyright (C) 2008, 2009 Genome Research Ltd. Author: Heng Li Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include "version.h" //#define MAQ_LONGREADS #ifdef MAQ_LONGREADS # define MAX_READLEN 128 #else # define MAX_READLEN 64 #endif #define MAX_NAMELEN 36 #define MAQMAP_FORMAT_OLD 0 #define MAQMAP_FORMAT_NEW -1 #define PAIRFLAG_FF 0x01 #define PAIRFLAG_FR 0x02 #define PAIRFLAG_RF 0x04 #define PAIRFLAG_RR 0x08 #define PAIRFLAG_PAIRED 0x10 #define PAIRFLAG_DIFFCHR 0x20 #define PAIRFLAG_NOMATCH 0x40 #define PAIRFLAG_SW 0x80 typedef struct { uint8_t seq[MAX_READLEN]; /* the last base is the single-end mapping quality. */ uint8_t size, map_qual, info1, info2, c[2], flag, alt_qual; uint32_t seqid, pos; int dist; char name[MAX_NAMELEN]; } maqmap1_t; typedef struct { int format, n_ref; char **ref_name; uint64_t n_mapped_reads; maqmap1_t *mapped_reads; } maqmap_t; maqmap_t *maq_new_maqmap(void) { maqmap_t *mm = (maqmap_t*)calloc(1, sizeof(maqmap_t)); mm->format = MAQMAP_FORMAT_NEW; return mm; } void maq_delete_maqmap(maqmap_t *mm) { int i; if (mm == 0) return; for (i = 0; i < mm->n_ref; ++i) free(mm->ref_name[i]); free(mm->ref_name); free(mm->mapped_reads); free(mm); } maqmap_t *maqmap_read_header(gzFile fp) { maqmap_t *mm; int k, len; mm = maq_new_maqmap(); gzread(fp, &mm->format, sizeof(int)); if (mm->format != MAQMAP_FORMAT_NEW) { if (mm->format > 0) { fprintf(stderr, "** Obsolete map format is detected. Please use 'mapass2maq' command to convert the format.\n"); exit(3); } assert(mm->format == MAQMAP_FORMAT_NEW); } if ( gzread(fp, &mm->n_ref, sizeof(int)) < sizeof(int)) { fprintf(stderr, "To few bytes in input file"); return NULL; } if (mm->n_ref < 0) { fprintf(stderr, "Incorrect n_ref read from input file\n"); return NULL; } if ( gzeof(fp) ) { fprintf(stderr, "Unexpected end of file."); return NULL; } mm->ref_name = (char**)calloc(mm->n_ref, sizeof(char*)); for (k = 0; k != mm->n_ref; ++k) { if ( gzeof(fp) ) { fprintf(stderr, "Unexpected end of file."); return NULL; } gzread(fp, &len, sizeof(int)); mm->ref_name[k] = (char*)malloc(len * sizeof(char)); if ( gzeof(fp) ) { fprintf(stderr, "Unexpected end of file."); return NULL; } gzread(fp, mm->ref_name[k], len); } /* read number of mapped reads */ if ( gzeof(fp) ) { fprintf(stderr, "Unexpected end of file."); return NULL; } gzread(fp, &mm->n_mapped_reads, sizeof(uint64_t)); return mm; } void maq2tam_core(gzFile fp, const char *rg) { maqmap_t *mm; maqmap1_t mm1, *m1; int ret; m1 = &mm1; mm = maqmap_read_header(fp); if (!mm) exit(1); while ((ret = gzread(fp, m1, sizeof(maqmap1_t))) == sizeof(maqmap1_t)) { int j, flag = 0, se_mapq = m1->seq[MAX_READLEN-1]; if (m1->flag) flag |= 1; if ((m1->flag&PAIRFLAG_PAIRED) || ((m1->flag&PAIRFLAG_SW) && m1->flag != 192)) flag |= 2; if (m1->flag == 192) flag |= 4; if (m1->flag == 64) flag |= 8; if (m1->pos&1) flag |= 0x10; if ((flag&1) && m1->dist != 0) { int c; if (m1->dist > 0) { if (m1->flag&(PAIRFLAG_FF|PAIRFLAG_RF)) c = 0; else if (m1->flag&(PAIRFLAG_FR|PAIRFLAG_RR)) c = 1; else c = m1->pos&1; } else { if (m1->flag&(PAIRFLAG_FF|PAIRFLAG_FR)) c = 0; else if (m1->flag&(PAIRFLAG_RF|PAIRFLAG_RR)) c = 1; else c = m1->pos&1; } if (c) flag |= 0x20; } if (m1->flag) { int l = strlen(m1->name); if (m1->name[l-2] == '/') { flag |= (m1->name[l-1] == '1')? 0x40 : 0x80; m1->name[l-2] = '\0'; } } printf("%s\t%d\t", m1->name, flag); printf("%s\t%d\t", mm->ref_name[m1->seqid], (m1->pos>>1)+1); if (m1->flag == 130) { int c = (int8_t)m1->seq[MAX_READLEN-1]; printf("%d\t", m1->alt_qual); if (c == 0) printf("%dM\t", m1->size); else { if (c > 0) printf("%dM%dI%dM\t", m1->map_qual, c, m1->size - m1->map_qual - c); else printf("%dM%dD%dM\t", m1->map_qual, -c, m1->size - m1->map_qual); } se_mapq = 0; // zero SE mapQ for reads aligned by SW } else { if (flag&4) printf("0\t*\t"); else printf("%d\t%dM\t", m1->map_qual, m1->size); } printf("*\t0\t%d\t", m1->dist); for (j = 0; j != m1->size; ++j) { if (m1->seq[j] == 0) putchar('N'); else putchar("ACGT"[m1->seq[j]>>6&3]); } putchar('\t'); for (j = 0; j != m1->size; ++j) putchar((m1->seq[j]&0x3f) + 33); putchar('\t'); if (rg) printf("RG:Z:%s\t", rg); if (flag&4) { // unmapped printf("MF:i:%d\n", m1->flag); } else { printf("MF:i:%d\t", m1->flag); if (m1->flag) printf("AM:i:%d\tSM:i:%d\t", m1->alt_qual, se_mapq); printf("NM:i:%d\tUQ:i:%d\tH0:i:%d\tH1:i:%d\n", m1->info1&0xf, m1->info2, m1->c[0], m1->c[1]); } } if (ret > 0) fprintf(stderr, "Truncated! Continue anyway.\n"); maq_delete_maqmap(mm); } int main(int argc, char *argv[]) { gzFile fp; if (argc == 1) { fprintf(stderr, "Version: %s\n", SAMTOOLS_VERSION); fprintf(stderr, "Usage: maq2sam []\n"); return 1; } fp = strcmp(argv[1], "-")? gzopen(argv[1], "r") : gzdopen(fileno(stdin), "r"); if (!fp ) { fprintf(stderr, "Unable to open file %s", argv[1]); fprintf(stderr, "Usage: maq2sam []\n"); return 1; } maq2tam_core(fp, argc > 2? argv[2] : 0); gzclose(fp); return 0; }