SUMMARISING RUN PARAMETERS
==========================
Input filename: nextera_100K.fastq.gz
Trimming mode: single-end
Trim Galore version: 0.6.4
Cutadapt version: 1.18
Number of cores used for trimming: 1
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Using Nextera adapter for trimming (count: 1960). Second best hit was smallRNA (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length before a sequence gets removed: 20 bp
Output file will be GZIP compressed


This is cutadapt 1.18 with Python 3.5.1
Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA nextera_100K.fastq.gz
Processing reads on 1 core in single-end mode ...
Finished in 3.33 s (33 us/read; 1.80 M reads/minute).

=== Summary ===

Total reads processed:                 100,000
Reads with adapters:                    37,813 (37.8%)
Reads written (passing filters):       100,000 (100.0%)

Total basepairs processed:     5,100,000 bp
Quality-trimmed:                   4,374 bp (0.1%)
Total written (filtered):      4,965,294 bp (97.4%)

=== Adapter 1 ===

Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 37813 times.

No. of allowed errors:
0-9 bp: 0; 10-12 bp: 1

Bases preceding removed adapters:
  A: 20.3%
  C: 30.5%
  G: 22.2%
  T: 26.9%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	19383	25000.0	0	19383
2	6510	6250.0	0	6510
3	2169	1562.5	0	2169
4	997	390.6	0	997
5	871	97.7	0	871
6	943	24.4	0	943
7	1020	6.1	0	1020
8	973	1.5	0	973
9	1120	0.4	0	1118 2
10	1010	0.1	1	994 16
11	717	0.0	1	713 4
12	473	0.0	1	467 6
13	324	0.0	1	318 6
14	276	0.0	1	274 2
15	271	0.0	1	263 8
16	164	0.0	1	162 2
17	89	0.0	1	86 3
18	75	0.0	1	71 4
19	53	0.0	1	46 7
20	63	0.0	1	58 5
21	38	0.0	1	38
22	49	0.0	1	44 5
23	39	0.0	1	35 4
24	38	0.0	1	28 10
25	29	0.0	1	26 3
26	12	0.0	1	12
27	6	0.0	1	3 3
28	4	0.0	1	3 1
29	2	0.0	1	1 1
30	9	0.0	1	8 1
31	15	0.0	1	1 14
32	5	0.0	1	0 5
33	2	0.0	1	0 2
34	4	0.0	1	0 4
37	3	0.0	1	0 3
38	1	0.0	1	0 1
39	5	0.0	1	0 5
40	4	0.0	1	0 4
41	3	0.0	1	0 3
42	2	0.0	1	0 2
43	5	0.0	1	0 5
44	2	0.0	1	0 2
45	9	0.0	1	0 9
46	1	0.0	1	0 1
48	7	0.0	1	0 7
49	5	0.0	1	0 5
50	1	0.0	1	0 1
51	12	0.0	1	10 2


RUN STATISTICS FOR INPUT FILE: nextera_100K.fastq.gz
=============================================
100000 sequences processed in total
Sequences removed because they became shorter than the length cutoff of 20 bp:	77 (0.1%)